MD707Z - Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric - Google Patents
Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric Download PDFInfo
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- MD707Z MD707Z MDS20130005A MDS20130005A MD707Z MD 707 Z MD707 Z MD 707Z MD S20130005 A MDS20130005 A MD S20130005A MD S20130005 A MDS20130005 A MD S20130005A MD 707 Z MD707 Z MD 707Z
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- Moldova
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- temperature
- nos3
- concentration
- dna
- primers
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- 238000000034 method Methods 0.000 title claims abstract description 29
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title abstract description 16
- 230000003511 endothelial effect Effects 0.000 title abstract description 4
- 108090000364 Ligases Proteins 0.000 title abstract description 3
- 101001128156 Homo sapiens Nanos homolog 3 Proteins 0.000 claims abstract description 29
- 101001124309 Homo sapiens Nitric oxide synthase, endothelial Proteins 0.000 claims abstract description 29
- 102100028452 Nitric oxide synthase, endothelial Human genes 0.000 claims abstract description 29
- 230000000692 anti-sense effect Effects 0.000 claims abstract description 12
- 239000011543 agarose gel Substances 0.000 claims abstract description 9
- 239000012634 fragment Substances 0.000 claims abstract description 9
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960005542 ethidium bromide Drugs 0.000 claims abstract description 7
- 238000004925 denaturation Methods 0.000 claims abstract description 6
- 230000036425 denaturation Effects 0.000 claims abstract description 6
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000908 ammonium hydroxide Substances 0.000 claims abstract description 5
- 108010075520 Nitric Oxide Synthase Type III Proteins 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 22
- 230000003321 amplification Effects 0.000 description 14
- 238000003199 nucleic acid amplification method Methods 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 11
- 108700028369 Alleles Proteins 0.000 description 10
- 238000002844 melting Methods 0.000 description 9
- 230000008018 melting Effects 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 238000000926 separation method Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000539 dimer Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 108091093088 Amplicon Proteins 0.000 description 3
- 102000008052 Nitric Oxide Synthase Type III Human genes 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 101150031207 NOS3 gene Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 208000031225 myocardial ischemia Diseases 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 208000004476 Acute Coronary Syndrome Diseases 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010071602 Genetic polymorphism Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108091092919 Minisatellite Proteins 0.000 description 1
- 102000008299 Nitric Oxide Synthase Human genes 0.000 description 1
- 108010021487 Nitric Oxide Synthase Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 206010042772 syncope Diseases 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD707Y MD707Y (en) | 2013-12-31 |
| MD707Z true MD707Z (ro) | 2014-07-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD707Z (mo) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2002110275A (ru) * | 2000-08-22 | 2004-03-27 | Дзе Брихэм Энд Уимен'З Хоспитал, Инк. (Us) | Диагностика и лечение сердечно-сосудистых состояний |
| EA007014B1 (ru) * | 2000-05-05 | 2006-06-30 | Апплайд Резеч Системз Арс Холдинг Н.В. | Применение il-18 в качестве диагностического маркера |
| RU2304775C2 (ru) * | 2005-07-27 | 2007-08-20 | Олег Сергеевич Глотов | Способ диагностики сердечно-сосудистых заболеваний и диагностический набор для его осуществления |
| RU2322193C1 (ru) * | 2006-12-04 | 2008-04-20 | Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации | Способ оценки генетического риска сердечно-сосудистых заболеваний у спортсменов |
| RU2007112316A (ru) * | 2007-04-03 | 2008-10-10 | Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научногоцентра Сибирского отделени Российской академии медицинских наук (RU) | Способ генетической диагностики подверженности к сердечно-сосудистым заболеваниям |
| EA200970781A1 (ru) * | 2007-02-21 | 2010-04-30 | Декоуд Дженетикс Ехф | Варианты генетической предрасположенности, связанные с сердечно-сосудистыми заболеваниями |
| RU2010141594A (ru) * | 2010-10-11 | 2012-04-20 | Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) | Способ генодиагностики сердечно-сосудистых заболеваний |
-
2013
- 2013-01-18 MD MDS20130005A patent/MD707Z/ro not_active IP Right Cessation
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA007014B1 (ru) * | 2000-05-05 | 2006-06-30 | Апплайд Резеч Системз Арс Холдинг Н.В. | Применение il-18 в качестве диагностического маркера |
| RU2002110275A (ru) * | 2000-08-22 | 2004-03-27 | Дзе Брихэм Энд Уимен'З Хоспитал, Инк. (Us) | Диагностика и лечение сердечно-сосудистых состояний |
| RU2304775C2 (ru) * | 2005-07-27 | 2007-08-20 | Олег Сергеевич Глотов | Способ диагностики сердечно-сосудистых заболеваний и диагностический набор для его осуществления |
| RU2322193C1 (ru) * | 2006-12-04 | 2008-04-20 | Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации | Способ оценки генетического риска сердечно-сосудистых заболеваний у спортсменов |
| EA200970781A1 (ru) * | 2007-02-21 | 2010-04-30 | Декоуд Дженетикс Ехф | Варианты генетической предрасположенности, связанные с сердечно-сосудистыми заболеваниями |
| RU2007112316A (ru) * | 2007-04-03 | 2008-10-10 | Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научногоцентра Сибирского отделени Российской академии медицинских наук (RU) | Способ генетической диагностики подверженности к сердечно-сосудистым заболеваниям |
| RU2376372C2 (ru) * | 2007-04-03 | 2009-12-20 | Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научного центра Сибирского отделения Российской академии медицинских наук | Способ генетической диагностики подверженности к сердечно-сосудистым заболеваниям |
| RU2010141594A (ru) * | 2010-10-11 | 2012-04-20 | Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) | Способ генодиагностики сердечно-сосудистых заболеваний |
Non-Patent Citations (1)
| Title |
|---|
| Caproş N., Istrati V., Popescu V., Butovscaia C., Popovici I. Polimorfismul genei sintetazei endoteliale de oxid nitric la pacienţii cu sindrom coronarian acut. Anale Ştiinţifice ale Universităţii de Stat de Medicină şi Farmacie "N.Testemiţanu". Probleme actuale în medicina internă. Zilele Universităţii 21-23 octombrie, ed. a X-a, Chişinău, 2009, vol. III, p. 57-62 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD707Y (en) | 2013-12-31 |
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