MD707Z - Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene - Google Patents

Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene

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Publication number
MD707Z
MD707Z MDS20130005A MDS20130005A MD707Z MD 707 Z MD707 Z MD 707Z MD S20130005 A MDS20130005 A MD S20130005A MD S20130005 A MDS20130005 A MD S20130005A MD 707 Z MD707 Z MD 707Z
Authority
MD
Moldova
Prior art keywords
temperature
polymorphic sequences
identifying
minutes
nitric oxide
Prior art date
Application number
MDS20130005A
Other languages
Romanian (ro)
Russian (ru)
Inventor
Ион ТОДЕРАШ
Александру МОВИЛЭ
Наталия КАПРОШ
Валериу ИСТРАТИ
Лидия ТОДЕРАШ
Original Assignee
Институт Зоологии Академии Наук Молдовы
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Зоологии Академии Наук Молдовы filed Critical Институт Зоологии Академии Наук Молдовы
Priority to MDS20130005A priority Critical patent/MD707Z/en
Publication of MD707Y publication Critical patent/MD707Y/en
Publication of MD707Z publication Critical patent/MD707Z/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to the medical molecular genetics, namely to a method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene.Summary of the method consists in that is carried out the whole blood sampling, is extracted DNA with 0.7 M ammonium hydroxide, is maintained at room temperature for 5 minutes and incubated in a thermostat at the temperature of 100°C for 30 minutes with continuous stirring, then the extracted the DNA is amplified using sense NOS3: 5'-GCCCTATGGTAGTAGTGCCTTT-3' and antisense NOS3 primers: 5'-SCTCTTAGTGCTGTGGTCA-3', at the same time is carried out the initial denaturation at the temperature of 94°C for 3 min, then are performed 30 cycles with denaturation at the temperature of 94°C for 1 min, at the temperature of 54°C for 30 seconds, at the temperature of 72°C for 30 seconds and at the temperature of 72°C for 5 minutes, are separated the amplified DNA fragments in 1.8% agarose gel, are stained with 0.5 µg/mL solution of ethidium bromide and are identified the polymorphic sequences.
MDS20130005A 2013-01-18 2013-01-18 Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene MD707Z (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDS20130005A MD707Z (en) 2013-01-18 2013-01-18 Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDS20130005A MD707Z (en) 2013-01-18 2013-01-18 Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene

Publications (2)

Publication Number Publication Date
MD707Y MD707Y (en) 2013-12-31
MD707Z true MD707Z (en) 2014-07-31

Family

ID=49912590

Family Applications (1)

Application Number Title Priority Date Filing Date
MDS20130005A MD707Z (en) 2013-01-18 2013-01-18 Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene

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MD (1) MD707Z (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2002110275A (en) * 2000-08-22 2004-03-27 Дзе Брихэм Энд Уимен'З Хоспитал, Инк. (Us) DIAGNOSIS AND TREATMENT OF CARDIOVASCULAR CONDITIONS
EA007014B1 (en) * 2000-05-05 2006-06-30 Апплайд Резеч Системз Арс Холдинг Н.В. Use of il-18 inhibitors as diagnostic marker
RU2304775C2 (en) * 2005-07-27 2007-08-20 Олег Сергеевич Глотов Method for predicting cardio-vascular diseases and diagnostic kit for its implementation
RU2322193C1 (en) * 2006-12-04 2008-04-20 Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации Method for evaluating genetic cardiovascular disease risk in sportsmen
RU2007112316A (en) * 2007-04-03 2008-10-10 Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научногоцентра Сибирского отделени Российской академии медицинских наук (RU) METHOD FOR GENETIC DIAGNOSIS OF PROSPECTS TO CARDIOVASCULAR DISEASES
EA200970781A1 (en) * 2007-02-21 2010-04-30 Декоуд Дженетикс Ехф OPTIONS OF GENETIC PREDISPOSITION ASSOCIATED WITH CARDIOVASCULAR DISEASES
RU2010141594A (en) * 2010-10-11 2012-04-20 Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) METHOD FOR GENODIAGNOSIS OF CARDIOVASCULAR DISEASES
  • 2013

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA007014B1 (en) * 2000-05-05 2006-06-30 Апплайд Резеч Системз Арс Холдинг Н.В. Use of il-18 inhibitors as diagnostic marker
RU2002110275A (en) * 2000-08-22 2004-03-27 Дзе Брихэм Энд Уимен'З Хоспитал, Инк. (Us) DIAGNOSIS AND TREATMENT OF CARDIOVASCULAR CONDITIONS
RU2304775C2 (en) * 2005-07-27 2007-08-20 Олег Сергеевич Глотов Method for predicting cardio-vascular diseases and diagnostic kit for its implementation
RU2322193C1 (en) * 2006-12-04 2008-04-20 Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации Method for evaluating genetic cardiovascular disease risk in sportsmen
EA200970781A1 (en) * 2007-02-21 2010-04-30 Декоуд Дженетикс Ехф OPTIONS OF GENETIC PREDISPOSITION ASSOCIATED WITH CARDIOVASCULAR DISEASES
RU2007112316A (en) * 2007-04-03 2008-10-10 Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научногоцентра Сибирского отделени Российской академии медицинских наук (RU) METHOD FOR GENETIC DIAGNOSIS OF PROSPECTS TO CARDIOVASCULAR DISEASES
RU2376372C2 (en) * 2007-04-03 2009-12-20 Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научного центра Сибирского отделения Российской академии медицинских наук Method for genetic diagnostics of susceptibility to cardiovascular diseases
RU2010141594A (en) * 2010-10-11 2012-04-20 Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) METHOD FOR GENODIAGNOSIS OF CARDIOVASCULAR DISEASES

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Caproş N., Istrati V., Popescu V., Butovscaia C., Popovici I. Polimorfismul genei sintetazei endoteliale de oxid nitric la pacienţii cu sindrom coronarian acut. Anale Ştiinţifice ale Universităţii de Stat de Medicină şi Farmacie "N.Testemiţanu". Probleme actuale în medicina internă. Zilele Universităţii 21-23 octombrie, ed. a X-a, Chişinău, 2009, vol. III, p. 57-62 *

Also Published As

Publication number Publication date
MD707Y (en) 2013-12-31

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