MD707Y - Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene - Google Patents
Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene Download PDFInfo
- Publication number
- MD707Y MD707Y MDS20130005A MD707Y MD 707 Y MD707 Y MD 707Y MD S20130005 A MDS20130005 A MD S20130005A MD 707 Y MD707 Y MD 707Y
- Authority
- MD
- Moldova
- Prior art keywords
- temperature
- polymorphic sequences
- identifying
- minutes
- nitric oxide
- Prior art date
Links
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108090000364 Ligases Proteins 0.000 title abstract 2
- 230000003511 endothelial effect Effects 0.000 title abstract 2
- 101001128156 Homo sapiens Nanos homolog 3 Proteins 0.000 abstract 2
- 101001124309 Homo sapiens Nitric oxide synthase, endothelial Proteins 0.000 abstract 2
- 102100028452 Nitric oxide synthase, endothelial Human genes 0.000 abstract 2
- 238000004925 denaturation Methods 0.000 abstract 2
- 230000036425 denaturation Effects 0.000 abstract 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 1
- 239000011543 agarose gel Substances 0.000 abstract 1
- 239000000908 ammonium hydroxide Substances 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 238000010241 blood sampling Methods 0.000 abstract 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 abstract 1
- 229960005542 ethidium bromide Drugs 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the medical molecular genetics, namely to a method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene.Summary of the method consists in that is carried out the whole blood sampling, is extracted DNA with 0.7 M ammonium hydroxide, is maintained at room temperature for 5 minutes and incubated in a thermostat at the temperature of 100°C for 30 minutes with continuous stirring, then the extracted the DNA is amplified using sense NOS3: 5'-GCCCTATGGTAGTAGTGCCTTT-3' and antisense NOS3 primers: 5'-SCTCTTAGTGCTGTGGTCA-3', at the same time is carried out the initial denaturation at the temperature of 94°C for 3 min, then are performed 30 cycles with denaturation at the temperature of 94°C for 1 min, at the temperature of 54°C for 30 seconds, at the temperature of 72°C for 30 seconds and at the temperature of 72°C for 5 minutes, are separated the amplified DNA fragments in 1.8% agarose gel, are stained with 0.5 µg/mL solution of ethidium bromide and are identified the polymorphic sequences.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (en) | 2013-01-18 | 2013-01-18 | Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (en) | 2013-01-18 | 2013-01-18 | Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD707Y true MD707Y (en) | 2013-12-31 |
| MD707Z MD707Z (en) | 2014-07-31 |
Family
ID=49912590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20130005A MD707Z (en) | 2013-01-18 | 2013-01-18 | Method for identifying the polymorphic sequences 4a/4b of the nitric oxide endothelial synthetase gene |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD707Z (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EE05056B1 (en) * | 2000-05-05 | 2008-08-15 | Applied Research Systems Ars Holding N.V. | Use of IL-18 inhibitors in the manufacture of a medicament for the treatment and / or prevention of atherosclerosis |
| EP1311547A2 (en) * | 2000-08-22 | 2003-05-21 | The Brigham And Women's Hospital, Inc. | Diagnosis and treatment of cardiovascular conditions |
| RU2304775C2 (en) * | 2005-07-27 | 2007-08-20 | Олег Сергеевич Глотов | Method for predicting cardio-vascular diseases and diagnostic kit for its implementation |
| RU2322193C1 (en) * | 2006-12-04 | 2008-04-20 | Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации | Method for evaluating genetic cardiovascular disease risk in sportsmen |
| AU2008218542B2 (en) * | 2007-02-21 | 2014-06-26 | Decode Genetics Ehf. | Genetic susceptibility variants associated with cardiovascular disease |
| RU2376372C2 (en) * | 2007-04-03 | 2009-12-20 | Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научного центра Сибирского отделения Российской академии медицинских наук | Method for genetic diagnostics of susceptibility to cardiovascular diseases |
| RU2010141594A (en) * | 2010-10-11 | 2012-04-20 | Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) | METHOD FOR GENODIAGNOSIS OF CARDIOVASCULAR DISEASES |
-
2013
- 2013-01-18 MD MDS20130005A patent/MD707Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD707Z (en) | 2014-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TK4Y | Erratum in official gazette with regard to short term patent |
Free format text: RECTIFICATION IN INID 72 |
|
| FG9Y | Short term patent issued | ||
| MK4Y | Short term patent expired |