MD707Y - Metoda de identificare a secventelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric - Google Patents
Metoda de identificare a secventelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric Download PDFInfo
- Publication number
- MD707Y MD707Y MDS20130005A MD707Y MD 707 Y MD707 Y MD 707Y MD S20130005 A MDS20130005 A MD S20130005A MD 707 Y MD707 Y MD 707Y
- Authority
- MD
- Moldova
- Prior art keywords
- temperature
- polymorphic sequences
- identifying
- minutes
- nitric oxide
- Prior art date
Links
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108090000364 Ligases Proteins 0.000 title abstract 2
- 230000003511 endothelial effect Effects 0.000 title abstract 2
- 101001128156 Homo sapiens Nanos homolog 3 Proteins 0.000 abstract 2
- 101001124309 Homo sapiens Nitric oxide synthase, endothelial Proteins 0.000 abstract 2
- 102100028452 Nitric oxide synthase, endothelial Human genes 0.000 abstract 2
- 238000004925 denaturation Methods 0.000 abstract 2
- 230000036425 denaturation Effects 0.000 abstract 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 1
- 239000011543 agarose gel Substances 0.000 abstract 1
- 239000000908 ammonium hydroxide Substances 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 238000010241 blood sampling Methods 0.000 abstract 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 abstract 1
- 229960005542 ethidium bromide Drugs 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Inventia se refera la genetica medicala moleculara, si anume la o metoda de identificare a secventelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric.Esenta metodei consta în aceea ca se colecteaza proba integrala de sânge, se extrage ADN cu hidroxid de amoniu 0,7 M, se mentine la temperatura camerei timp de 5 min si se incubeaza într-un termostat la temperatura de 100ºC timp de 30 min cu agitare continua, apoi ADN extras se amplifica cu utilizarea primerilor NOS3 sens: 5´-GCCCTATGGTAGTAGTGCCTTT-3´ si NOS3 antisens: 5´- CCTCTTAGTGCTGTGGTCA-3´, totodata se efectueaza denaturarea initiala la temperatura de 94°C timp de 3 min, apoi se efectueaza 30 de cicluri cu denaturarea la temperatura de 94°C timp de 1 min, la temperatura de 54°C timp de 30 s, la temperatura de 72°C timp de 30 s si la temperatura de 72°C timp de 5 min, se separa fragmentele amplificate de ADN în gel de agaroza de 1,8%, se coloreaza cu o solutie de bromura de etidiu de 0,5 µg/mL si se identifica secventele polimorfe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD707Y true MD707Y (ro) | 2013-12-31 |
| MD707Z MD707Z (ro) | 2014-07-31 |
Family
ID=49912590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20130005A MD707Z (ro) | 2013-01-18 | 2013-01-18 | Metodă de identificare a secvenţelor polimorfe 4a/4b ale genei sintetazei endoteliale a oxidului nitric |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD707Z (ro) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040076628A1 (en) * | 2000-05-05 | 2004-04-22 | Yolande Chvatchko | Use of IL-18 inhibitors for the treatment and/or prevention of atherosclerosis |
| BR0107157A (pt) * | 2000-08-22 | 2002-07-16 | Brigham And Womens Hospital In | Diagnose e tratamento de condições cardiovasculares |
| RU2304775C2 (ru) * | 2005-07-27 | 2007-08-20 | Олег Сергеевич Глотов | Способ диагностики сердечно-сосудистых заболеваний и диагностический набор для его осуществления |
| RU2322193C1 (ru) * | 2006-12-04 | 2008-04-20 | Федеральное государственное учреждение "Учебно-научный медицинский центр" Управления делами Президента Российской Федерации | Способ оценки генетического риска сердечно-сосудистых заболеваний у спортсменов |
| WO2008102380A1 (en) * | 2007-02-21 | 2008-08-28 | Decode Genetics Ehf | Genetic susceptibility variants associated with cardiovascular disease |
| RU2376372C2 (ru) * | 2007-04-03 | 2009-12-20 | Государственное учреждение Научно-исследовательский институт медицинской генетики Томского научного центра Сибирского отделения Российской академии медицинских наук | Способ генетической диагностики подверженности к сердечно-сосудистым заболеваниям |
| RU2010141594A (ru) * | 2010-10-11 | 2012-04-20 | Российская Федерация в лице Министерства образования и науки Российской Федерации (RU) | Способ генодиагностики сердечно-сосудистых заболеваний |
-
2013
- 2013-01-18 MD MDS20130005A patent/MD707Z/ro not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD707Z (ro) | 2014-07-31 |
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Free format text: RECTIFICATION IN INID 72 |
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| FG9Y | Short term patent issued | ||
| MK4Y | Short term patent expired |