KR970704872A - 개선된 세척 조성물 - Google Patents

개선된 세척 조성물 Download PDF

Info

Publication number
KR970704872A
KR970704872A KR1019970700864A KR19970700864A KR970704872A KR 970704872 A KR970704872 A KR 970704872A KR 1019970700864 A KR1019970700864 A KR 1019970700864A KR 19970700864 A KR19970700864 A KR 19970700864A KR 970704872 A KR970704872 A KR 970704872A
Authority
KR
South Korea
Prior art keywords
amylase
alpha
mutant
cleaning composition
protease
Prior art date
Application number
KR1019970700864A
Other languages
English (en)
Inventor
크리스토퍼 씨 바네트
콜린 미친슨
스콧 디 파우워
Original Assignee
혼 마가렛 에이
제넨코, 인터내셔널 아이엔씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 혼 마가렛 에이, 제넨코, 인터내셔널 아이엔씨 filed Critical 혼 마가렛 에이
Publication of KR970704872A publication Critical patent/KR970704872A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3942—Inorganic per-compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3947—Liquid compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Abstract

본 발명은 기술된 자연 발생 알파 - 아밀라제 또는 재조합 알파 - 아밀라제의 DNA 서열에서 얻어진 신규한 알파 - 아밀라제 돌연 변이체에 관한다. 일반적으로, 상기 돌연 변이체 알파 - 아밀라제는 자연 발생 알파- 아밀라제 또는 재조합 알파 - 아밀라제를 암호화하는 전구체 DNA 서열을 시험관에서 개질시켜 전구체 알파- 아밀라제의 아미소산 서열내 하나 이상의 산화 가능한 아미노산 잔기를 결실시키거나 또는 치환(대체)시켜 얻을 수 있다. 이러한 돌연 변이체 알파 - 아밀라제는 산화 안정도 및 / 또는 pH 성능 및 / 또는 내열성이 전구체에 비하여 개질되었다. 또한 기술된 것들은 돌연 변이체 아밀라제를 포함하는 세제 및 녹말 액화 조성물일 뿐만아니라 돌연 변이체 아밀라제를 사용하는 방법이다. 더욱 구체적으로 상기 돌연 변이체 알파 - 아밀라제는 MET197 또는 MET15 위치에서 또는 TRP138 잔기 또는 다른 미생물 공급원(바실러서, 아스퍼질러스)에서 얻은 다른 알파 - 아밀라제 잔기들이 개질된 바실러스 라이커니포르미스에서 얻은 바람직한 돌연 변이체 알파-아밀라제이다.

Description

개선된 세척 조성물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 비, 라이커니포르미스(NCIB 8061)에서 얻은 알파 - 아밀라제 유전자의 DNA 서열을 나타내는 것으로서, Gray, G와 그의 동료들에 의하여 (1986) J. Bacter. 166 : 635 - 643 에 기술된 바와같은 서열 제31번 및 추론에 의한 이의 번역 생성물을 나타낸다.
제2도는 비, 라이커니포르미스(NCIB 8061)에서 얻은 성숙한 알파-아밀라제 효소의 아미노산 서열로서, 서열 제32번을 나타낸다.
제3도는 바실러스 알파-아밀라제의 1차 구조의 정렬을 나타낸다. 상기 비, 라이커니포르미스 아밀라제(Am-Lich), 서열 제33번은 G.ray, G 와 그의 동료에 의하여 (1986), J.Bact. 166 : 635 - 643에 기술되었으며; 비, 아밀로라이커니패이션스 아밀라제(Am - Amylo), 서열 제34번은 Takkinen, K. 와 그의 동료에 의하여 (1983). J,. Biol. Chem. 258 : 1007 - 1013 에 기술되었으며 : 비 스테아로서모필러스(Am - Stearo), 서열 제35번은 Ihara, H 와 그의 동료들에 의하여(1985) J.Biochem. 98 : 95 - 103 에 기술되었다.
제4a도는 성숙한 알파 - 아밀라제 변이체 M 197 T의 아미노산 서열로서, 서열 제36번을 나타낸다.
제4b도는 비, 라이커니포르미스 NCIB 8061에서 얻은 알파 - 아밀라제의 A4의 형의 아미노산 서열로서, 서열 제37번을 나타낸다. 번 호 메김은 4개의 추가적 알라닌으로 시작되는 N- 말단에서 시작하였다.
제5도는 pA4BL을 나타내는 것으로서, BLA4, 비. 라이커니포르미스 알파-아밀라제 유전자이며, PstI 에서 SstI 사이인 AmpR는 pBR 322에서 얻은 암피실린 내성 유전자 : 및 CAT는 pC 194에서 얻은 클로르 암페니콜 내성 유전자이다.
제8도는 M197 카세트 돌연 변이체의 생성 방법에 대한 개략도이다.

Claims (7)

  1. 비.라이커니포르미스(B. licheniformis) 내 M+197 위치의 메티오닌 및 비. 라이커니포르미스(B. Bcheniformis)알파- 아밀라제 내의 M+15 또는 W+138 위치의 하나 이상의 메티오닌 또는 트립토판을 치환시킴으로써 전구체 알파 아밀라제에서 얻은, 알파 아밀라제를 암호화하는 돌연 변이된 DNA서열의 발현 생성물인 돌연 변이체 알파 - 아밀라제를 표백제 - 함유 조성물에 가하는 것을 개선점으로 포함하는 개선된 표백제- 함유 세척 조성물.
  2. 제1항에 있어서, 세척 조성물이 접시 보호 세척 조성물(dish care cleaning composition)인 개선된 세척 조성물.
  3. 제l항에 있어서, 돌연 변이체 알파 - 아밀라제가 M15T/ M197T; M15S/ M197T; W138Y / M197T;M15S/W138Y/M197T 및 M15T/ W138Y/ M197T를 포함하는 그룹에서 선택된 개선된 세척 조성물.
  4. 제1항에 있어서, 바실러스 아밀로라이크패이션스(B. amylolichefacience) 프로테아제 M+222 위치의 메티오닌을 치환시킴으로써 전구체 프로테아제에서 얻어진, 프로테아제를 암호화하는 돌연 변이된 DNA 서열의 발현 생성물인 돌연 변이체 프로테아제를 추가로 포함하는 개선된 세척 조성물.
  5. 제4항에 있어서, 돌연 변이체 프로테아제가 알라닌, 시스테인 및 세린을 포함하는 아미노산 그룹에서 선택된 치환체를 포함하는 개선된 세척 조성물.
  6. 제4항에 있어서, M15T/ M197T, M15S/ M197T, W138Y/ M197T 및 M15T/ W138Y/ M197T 및 M15T/W138Y/M197T을 포함하는 그룹에서 선택된 알파-아밀라제 돌연 변이체 및 M222C, M222S 및 M222A를 포함하는 그룹에서 선택된 프로테아제 돌연 변이체를 포함하는 개선된 세척 조성물.
  7. 제6항에 있어서, 압자형 조성물인 개선된 세척 조성물.
KR1019970700864A 1994-08-11 1995-08-09 개선된 세척 조성물 KR970704872A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28935194A 1994-08-11 1994-08-11
US08/289,351 1994-08-11
PCT/US1995/010426 WO1996005295A2 (en) 1994-08-11 1995-08-09 An improved cleaning composition

Publications (1)

Publication Number Publication Date
KR970704872A true KR970704872A (ko) 1997-09-06

Family

ID=23111158

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019970700864A KR970704872A (ko) 1994-08-11 1995-08-09 개선된 세척 조성물

Country Status (15)

Country Link
EP (1) EP0775201A2 (ko)
JP (1) JPH10504197A (ko)
KR (1) KR970704872A (ko)
CN (1) CN1158637A (ko)
AU (1) AU686007B2 (ko)
BR (1) BR9508582A (ko)
CA (1) CA2197203A1 (ko)
CO (1) CO4440440A1 (ko)
FI (1) FI970563A0 (ko)
HU (1) HUT77748A (ko)
MX (1) MX9700776A (ko)
NO (1) NO970609L (ko)
NZ (1) NZ291984A (ko)
PL (1) PL318209A1 (ko)
WO (1) WO1996005295A2 (ko)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0815193A1 (en) * 1995-03-24 1998-01-07 Genencor International Inc. An improved laundry detergent composition comprising amylase
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
WO1999064619A2 (en) 1998-06-10 1999-12-16 Novozymes A/S Novel mannanases
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
JP2010528606A (ja) * 2007-05-30 2010-08-26 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン バチルス・リケニフォルミス（ＢａｃｉｌｌｕｓＬｉｃｈｅｎｉｆｏｒｍｉｓ）アルファ−アミラーゼの改善された変異種
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
ES2692544T3 (es) 2011-06-30 2018-12-04 Novozymes A/S Variantes de alfa-amilasa
EP3004314B1 (en) 2013-05-29 2018-06-20 Danisco US Inc. Novel metalloproteases
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
EP3110833B1 (en) 2013-05-29 2020-01-08 Danisco US Inc. Novel metalloproteases
EP3260538B1 (en) 2013-05-29 2021-04-14 Danisco US Inc. Novel metalloproteases
EP3514230B1 (en) 2013-12-13 2021-09-22 Danisco US Inc. Serine proteases of bacillus species
EP3080263B1 (en) 2013-12-13 2019-07-03 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
MX2016007759A (es) 2013-12-16 2016-08-19 Du Pont Uso de eteres de poli-alfa-1,3-glucano como modificadores de la viscosidad.
US9957334B2 (en) 2013-12-18 2018-05-01 E I Du Pont De Nemours And Company Cationic poly alpha-1,3-glucan ethers
US20150232785A1 (en) 2014-02-14 2015-08-20 E I Du Pont De Nemours And Company Polysaccharides for viscosity modification
JP2017515921A (ja) 2014-03-11 2017-06-15 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 酸化されたポリα−１，３−グルカン
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3919599A1 (en) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3207129B1 (en) 2014-10-17 2019-11-20 Danisco US Inc. Serine proteases of bacillus species
EP3212783A1 (en) 2014-10-27 2017-09-06 Danisco US Inc. Serine proteases
WO2016069552A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
US20180010074A1 (en) 2014-10-27 2018-01-11 Danisco Us Inc. Serine proteases of bacillus species
WO2016069548A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
EP3550017B1 (en) 2014-10-27 2021-07-14 Danisco US Inc. Serine proteases
CN107109450A (zh) 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
FI3307427T3 (fi) 2015-06-09 2023-11-09 Danisco Us Inc Osmoottiset puhkeavat kapselit
EP3310911B1 (en) 2015-06-17 2023-03-15 Danisco US Inc. Bacillus gibsonii-clade serine proteases
EP3371307A1 (en) 2015-11-05 2018-09-12 Danisco US Inc. Paenibacillus and bacillus spp. mannanases
BR112018008946A2 (pt) 2015-11-05 2020-11-03 Danisco Us Inc. mananases de paenibacillus sp.
US10822574B2 (en) 2015-11-13 2020-11-03 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
DK3387124T3 (da) 2015-12-09 2021-08-23 William Cuevas Kombinatoriske alfa-amylasevarianter
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
BR112018072586A2 (pt) 2016-05-05 2019-02-19 Danisco Us Inc variantes de protease e usos das mesmas
BR112018074700A2 (pt) 2016-05-31 2019-10-01 Danisco Us Inc variantes de protease e usos das mesmas
JP7152319B2 (ja) 2016-06-17 2022-10-12 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
CN110312794B (zh) 2016-12-21 2024-04-12 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
EP3559227A1 (en) 2016-12-21 2019-10-30 Danisco US Inc. Protease variants and uses thereof
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
WO2018183662A1 (en) 2017-03-31 2018-10-04 Danisco Us Inc Delayed release enzyme formulations for bleach-containing detergents
CA3067837A1 (en) 2017-06-30 2019-01-03 Danisco Us Inc Low-agglomeration, enzyme-containing particles
WO2019108599A1 (en) 2017-11-29 2019-06-06 Danisco Us Inc Subtilisin variants having improved stability
BR112020012584A2 (pt) 2017-12-21 2020-11-24 Danisco Us Inc. grânulos fundidos a quente contendo enzima que compreendem um dessecante termotolerante
BR112020016068A2 (pt) 2018-02-08 2020-12-08 Danisco Us Inc. Partículas cera termicamente resistente matriz para encapsulamento de enzima
US20210214703A1 (en) 2018-06-19 2021-07-15 Danisco Us Inc Subtilisin variants
US20210363470A1 (en) 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
US20210189295A1 (en) 2018-08-30 2021-06-24 Danisco Us Inc Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
EP3980517A1 (en) 2019-06-06 2022-04-13 Danisco US Inc. Methods and compositions for cleaning
CN116323935A (zh) 2020-08-27 2023-06-23 丹尼斯科美国公司 用于清洁的酶和酶组合物
US11926855B2 (en) * 2020-08-28 2024-03-12 Kerry Group Services International Limited Feed composition supplemented with a protease combination
US20240117275A1 (en) 2021-01-29 2024-04-11 Danisco Us Inc. Compositions for cleaning and methods related thereto
CN117616120A (zh) 2021-06-30 2024-02-27 丹尼斯科美国公司 变体脂肪酶及其用途
CN117916354A (zh) 2021-09-03 2024-04-19 丹尼斯科美国公司 用于清洁的衣物洗涤组合物
WO2023039270A2 (en) 2021-09-13 2023-03-16 Danisco Us Inc. Bioactive-containing granules
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
JP3678309B2 (ja) * 1992-07-23 2005-08-03 ノボザイムス アクティーゼルスカブ 突然変異α−アミラーゼ、洗剤、皿洗い剤及び液化剤
CN1104499C (zh) * 1993-02-11 2003-04-02 吉恩康国际有限公司 氧化作用稳定的α－淀粉酶
BR9407767A (pt) * 1993-10-08 1997-03-18 Novo Nordisk As Variante de enzima &-amilase uso da mesma construção de DNA vetor de express o recombinante célula processos para produzir uma &-amilase hibrida e para preparar uma variante de uma &-amilase aditivo detergente e composições detergentes

Also Published As

Publication number Publication date
FI970563A (fi) 1997-02-10
NZ291984A (en) 1998-04-27
HUT77748A (hu) 1998-07-28
NO970609D0 (no) 1997-02-10
NO970609L (no) 1997-03-24
WO1996005295A2 (en) 1996-02-22
EP0775201A2 (en) 1997-05-28
CO4440440A1 (es) 1997-05-07
FI970563A0 (fi) 1997-02-10
AU3366295A (en) 1996-03-07
AU686007B2 (en) 1998-01-29
JPH10504197A (ja) 1998-04-28
CN1158637A (zh) 1997-09-03
CA2197203A1 (en) 1996-02-22
WO1996005295A3 (en) 1996-03-28
MX9700776A (es) 1997-05-31
PL318209A1 (en) 1997-05-26
BR9508582A (pt) 1998-06-02

Similar Documents

Publication Publication Date Title
KR970704872A (ko) 개선된 세척 조성물
CA2253589C (en) Modified .alpha.-amylases having altered calcium binding properties
CA2452029C (en) A group of .alpha.-amylases and a method for identification and production of .alpha.-amylases
KR960701197A (ko) 산화 안정성 알파-아밀라아제(oxidatively stable alpha-amylase)
Vasantha et al. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein
Aiba et al. Cloning and expression of thermostable α-amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis
EP0850307B1 (en) Alkaliphilic and thermophilic microorganisms and enzymes obtained therefrom
JP4358431B2 (ja) α−アミラーゼ変異体
MX9706823A (es) Una composicion mejorada de detergente para lavar ropa que contiene amilasa.
DE3478469D1 (en) Maltogenic amylase enzyme product, preparation and use thereof
ATE293696T1 (de) Mutierte alpha-amylase enzyme mit erhöhter stabilität
Kwon et al. Extracellular alkaline proteases from alkalophilic Vibrio metschnikovii strain RH530
ATE90965T1 (de) Chimaere enzyme.
Satoh et al. Purification, characterization, and nucleotide sequence of an intracellular maltotriose-producing alpha-amylase from Streptococcus bovis 148
JPH05507283A (ja) プロテアーゼ及びインヒビターを含む洗浄剤並びにそれに利用する新規なるインヒビター
Uozumi et al. A single gene directs synthesis of a precursor protein with beta-and alpha-amylase activities in Bacillus polymyxa
EP0648843A1 (en) DNA encoding a hyperthermostable alpha-amylase
Momma Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae
CN1222938A (zh) 经修饰具有改变了的钙离子吉合性质的α－淀粉酶
WO1996035794A1 (en) Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities
Diderichsen et al. Cloning and expression of an amylase gene from Bacillus stearothermophilus
EP0261009B1 (fr) Sequence d'ADN exercant une fonction se manifestant par une surproduction de protéines exocellulaires par diverses souches de bacillus, et vecteurs contenant cette séquence
Kakudo et al. Secretory expression of a glutamic-acid-specific endopeptidase (SPase) from Staphylococcus aureus ATCC12600 in Bacillus subtilis
INATOMI The subunit structure of nitrite reductase purified from the denitrifier Achromobacter cycloclastes
Maeda et al. Nucleotide sequence of the neutral protease gene (nprL) from Lactobacillus sp. and characterization of the enzyme

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid