KR950700405A - 엔도-1,4-β-D-글루카나제(Endo-1,4-Beta-D-Glucanase) - Google Patents

엔도-1,4-β-D-글루카나제(Endo-1,4-Beta-D-Glucanase)

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KR950700405A
KR950700405A KR1019940703020A KR19940703020A KR950700405A KR 950700405 A KR950700405 A KR 950700405A KR 1019940703020 A KR1019940703020 A KR 1019940703020A KR 19940703020 A KR19940703020 A KR 19940703020A KR 950700405 A KR950700405 A KR 950700405A
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sequence
enzyme
host cell
vector
pct
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KR100271594B1 (ko
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잭클린 드 실바
칼 두들리 자르맨
데이비드 앤드류 애로우스미쓰
존 스펜스 그랜트 리이드
메리 엘리자베쓰 에드워즈
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알.브이.타테
유니레버 엔.브이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01073—Licheninase (3.2.1.73)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S47/00—Plant husbandry
    • Y10S47/01—Methods of plant-breeding and including chromosome multiplication

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  • Life Sciences & Earth Sciences (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
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Abstract

본 발명은 크실로글루칸-특이 엔도-(1,4)-β-D-글루카나제 활성을 갖는 효소를 암호화하는 서열 및 그의 기능적 동등 서열에 관한 것이다. 또한, 상기 서열로 이루어지는 벡터, 상기 서열이 도입된 변종 식물, 식물 특성의 변경방법, 및 재조합 DNA 기법에 의해 상기 정의한 활성을 갖는 효소의 제조방법에 관한 것이다.

Description

엔도-1,4-β-D-글루카나제(Endo-1,4-Beta-D-Glucanase)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1 및 2도는 조(組)(제1A도) 또는 친화 정제된(제1B 또는 2도)항 크실로글루카나제 항체로 탐침화한 면역 블롯의 사진이고, 제 3 도는 항 크실로글루카나제 항체(레인 1 내지 3)를 사용하여 면역 침전시킨 한련종자 RNA의 시험관내 번역 산물(레인 5 및 6)의 SDS-PAGE 분석 사진이고,제 4 도는 한편 종자에서 크실로글루카나제 발현 수준 대 시간의 관계를 나타내고, 제 5 도는 한편 cDNA클론의 제한 효소 분석 결과를 나타낸다.

Claims (13)

  1. 제 9 도(서열 번호1)에 나타낸 서열의 뉴클레오티드 35 내지 919로 이루어지는, 크실로글루칸- 특이 엔도-(1,4)-β-D-글루카나제 활성을 갖는 효소를 암호화 하는 뉴클레오티드 서열 또는 그의 기능적 동등 서열.
  2. 제 1 항에 있어서, 5′-비번역 영역을 더 포함하는 서열.
  3. 제 1 항 또는 제 2 항에 있어서, 3′-비번역 영역을 더 포함하는 서열.
  4. 제 1 항 내지 제 3 항 중 어느 한 항에 있어서, 한련(Topaeolum majus L.)으로부터 얻는 서열을 포함하는 서열.
  5. 제 1 항 내지 제 4 항 중 어느 한항에 서열로 이루어지는 벡터.
  6. 제 1 항 내지 제 4 항 중 어느 한항의 서열리 도입된 식물 전체 또는 그의 일부.
  7. 제 1 항 내지 제 4 항 중 어느 한항의 서열을 도입하는 것으로 이루어지는 식물 전체 또는 그의 일부의 특성의 변경방법.
  8. 제 7 항에 있어서, 상기 변경되는 특성에 크기, 조직 또는 성숙 속도가 포함되는 방법.
  9. 제 7 항 또는 제 8 항에 있어서, 과실 또는 채소의 특성이 변경되는 방법.
  10. 제 7 항 내지 제 9 항 중 어느 한 항에 있어서, 토마토 식물 전체 또는 그의 일부의 특성이 변경되는 방법.
  11. 제 1 항 내지 제 4 항 중 어느 한 항의 서열을 적합한 벡터에 삽입하는 단계; 상기 벡터로 적당한 숙주세포를 형질 전환시키는 단계; 효소가 발현되는 적합한 배양 조건에서 상기 형질 전환 숙주 세포를 성장시키는 단계; 배양 배지 및(또는) 숙주 세포로부터 효소를 얻는 단계로 이루어지는 크시로글루칸-특이 엔도 -(1,4)-β-D-글루카나제 활성을 갖는 효소의 제조방법.
  12. 제11항에 있어서, 숙주 세포가 미생물인 방법.
  13. 제11항 또는 제12항에 있어서, 숙주 세포가 진핵 생물인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703020A 1992-02-28 1993-03-01 엔도-1, 4-베타-d-글루카나제 KR100271594B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92301707.3 1992-02-28
EP92301707 1992-02-28
PCT/GB1993/000424 WO1993017101A1 (en) 1992-02-28 1993-03-01 Endo-1,4-beta-d-glucanase

Publications (2)

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KR950700405A true KR950700405A (ko) 1995-01-16
KR100271594B1 KR100271594B1 (ko) 2000-11-15

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Country Link
US (1) US5767364A (ko)
EP (1) EP0628075B1 (ko)
JP (1) JPH07508159A (ko)
KR (1) KR100271594B1 (ko)
AT (1) ATE209677T1 (ko)
AU (2) AU3572893A (ko)
CA (1) CA2117608A1 (ko)
DE (1) DE69331226T2 (ko)
ES (1) ES2164655T3 (ko)
MX (1) MX9301120A (ko)
WO (1) WO1993017101A1 (ko)
ZA (1) ZA931333B (ko)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0675951T3 (da) 1992-12-23 2003-09-08 Novozymes As Enzym med endoglucanaseaktivitet
DK154492D0 (da) * 1992-12-23 1992-12-23 Novo Nordisk As Enzym
GB9323225D0 (en) * 1993-11-10 1994-01-05 Unilever Plc Novel plant enzyme
AU3395995A (en) * 1994-09-09 1996-03-27 Unilever Plc Xyloglucan-specific beta-galactosidase
CN1105211C (zh) * 1995-12-21 2003-04-09 诺沃奇梅兹有限公司 提供具有改进的强度和/或形状保持性能和/或抗皱性能的纤维质原料的方法及由此得到的纤维质原料
FR2763077B1 (fr) * 1997-05-07 1999-08-13 Agronomique Inst Nat Rech Controle de la croissance cellulaire chez une plante par l'utilisation d'un gene d'endo-1,4-beta-d-glucanase
DE69833197T2 (de) * 1997-07-07 2006-09-14 Novozymes A/S Alkalische xyloglukanase
US6268197B1 (en) 1997-07-07 2001-07-31 Novozymes A/S Xyloglucan-specific alkaline xyloglucanase from bacillus
US6489279B2 (en) 1998-05-05 2002-12-03 The Procter & Gamble Company Laundry and cleaning compositions containing xyloglucanase enzymes
AU4707899A (en) * 1998-06-23 2000-01-10 Pioneer Hi-Bred International, Inc. Alteration of hemicellulose concentration in plants by rgp
US6878860B1 (en) * 1999-07-06 2005-04-12 Senesco, Inc. DNA encoding a plant deoxyhypusine synthase, a plant eukaryotic initiation factor 5A, transgenic plants and a method for controlling senescence programmed and cell death in plants
US7358418B2 (en) * 1999-07-06 2008-04-15 Senesco Technologies, Inc. Isoforms of eIF-5A: senescence-induced eLF5A; wounding-induced eIF-4A; growth eIF-5A; and DHS
US20060294623A1 (en) * 1999-07-06 2006-12-28 Thompson John E Isolated eIF-5A and polynucleotides encoding same
US6538182B1 (en) * 1999-07-06 2003-03-25 Senesco, Inc. DNA encoding a plant deoxyhypusine synthase, a plant eukaryotic initiation factor 5A, transgenic plants and a method for controlling senescence programmed and cell death in plants
WO2001012794A1 (en) * 1999-08-13 2001-02-22 Novozymes A/S Alkaline xyloglucanase from malbranchea
KR100396210B1 (ko) * 2000-10-13 2003-09-17 학교법인고려중앙학원 고추 한별 품종의 글루칸가수분해효소 유전자 및 이를이용한 식물병 저항성 탐색방법
US7632654B2 (en) * 2002-04-29 2009-12-15 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Means and methods for detecting endoglycosidase activity

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340685C (en) * 1988-07-29 1999-07-27 Frederick Meins Dna sequences encoding polypeptides having beta-1,3-glucanase activity
WO1990009436A1 (en) * 1989-02-16 1990-08-23 Carlsberg A/S A THERMOSTABLE (1,3-1,4)-β-GLUCANASE
JPH03180180A (ja) * 1989-12-08 1991-08-06 Ajinomoto Co Inc 植物細胞壁由来エンド―１，４―β―グルカナーゼとその抗体
US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
US5516694A (en) * 1992-03-26 1996-05-14 Takara Shuzo Co., Ltd. Endo-xyloglucan transferase
US5569830A (en) * 1994-05-03 1996-10-29 Regents Of The University Of California Plant inhibitors of fungal polygalacturonases and their use to control fungal disease

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Publication number Publication date
ATE209677T1 (de) 2001-12-15
MX9301120A (es) 1993-09-01
DE69331226T2 (de) 2002-07-11
DE69331226D1 (de) 2002-01-10
EP0628075A1 (en) 1994-12-14
US5767364A (en) 1998-06-16
ZA931333B (en) 1994-08-25
AU3529597A (en) 1997-11-20
WO1993017101A1 (en) 1993-09-02
KR100271594B1 (ko) 2000-11-15
AU3572893A (en) 1993-09-13
ES2164655T3 (es) 2002-03-01
JPH07508159A (ja) 1995-09-14
CA2117608A1 (en) 1993-09-02
EP0628075B1 (en) 2001-11-28

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