KR950023707A - 5'-이노신산(5'-Inosinic Acid)을 생산하는 미생물 - Google Patents

5'-이노신산(5'-Inosinic Acid)을 생산하는 미생물 Download PDF

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KR950023707A
KR950023707A KR1019940000323A KR19940000323A KR950023707A KR 950023707 A KR950023707 A KR 950023707A KR 1019940000323 A KR1019940000323 A KR 1019940000323A KR 19940000323 A KR19940000323 A KR 19940000323A KR 950023707 A KR950023707 A KR 950023707A
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inosinic acid
microorganism
guanine
xanthine
adenine
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KR1019940000323A
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KR970010566B1 (ko
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정성오
한종권
심재익
오윤석
고형곤
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김정순
제일제당 주식회사
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium

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Abstract

본 발명은 5′-이노신산을 생산하는 미생물에 관한 것으로, 좀더 구체적으로는 브레비박테리움 암모니아게네스(ATCC 6872)의 변이주로서 종래 5'-이노신산을 생산하는 아데닌(Adenine) 노출형 변이주[Agr. Bio. Chem., Vol; 47(5), p1035-1041, 1983, (KY 13102, KY 13171, KY 13184등)] 또는 아데닌과 크산틴(Xanthine) 또는 구아닌(Guanine) 동시 요구성 균주와 달리 본 발명에 따른 미생물은 아데닌을 요구하는 반면 크산틴 또는 구아닌을 요구하지 않으나 이들을 첨가함으로써 생육이 촉진되며, 우레아(Urea)를 자화할 수 있는 우레아제(Urease)가 결손되고, 세포내에 생성된 다량의 5′-이노신산을 세포밖으로 잘 분비할 수 있도록 세포벽 분해효소인 라이소자임(Lysozyme)에 감수성이 높으며, 삼투압 내성형질이 부여된 미생물로서, 값싼 당질을 원료로 사용이 가능하며 질소원 제한 영양배지에서 호기적으로 배양하며 5'-이노신산을 고수율, 고농도록 배양액중에 직접 축적시키는 미생물에 관한 것이다.

Description

5′-이노신산(5'-Inosinic Acid)을 생산하는 미생물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (1)

  1. 생육시 아데닌과 비오틴을 요구하며 구아닌 또는 크산틴 누출형 변이주로서 세포벽 분해 효소인 라이소자임에 감수성이 높고, 3, 4-디하이드로 프로린의 농도가 3500㎍/ml 이상에서도 생육이 가능한 삼투압 내성을 갖음을 특징으로 하는 5′-이노신산의 생성능이 있는 브레비박테리움 암모니아게네스 KFCC 10814 미생물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940000323A 1994-01-10 1994-01-10 5'-이노신산(5'-lnosinic Acid)을 생산하는 미생물 KR970010566B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940000323A KR970010566B1 (ko) 1994-01-10 1994-01-10 5'-이노신산(5'-lnosinic Acid)을 생산하는 미생물

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940000323A KR970010566B1 (ko) 1994-01-10 1994-01-10 5'-이노신산(5'-lnosinic Acid)을 생산하는 미생물

Publications (2)

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KR950023707A true KR950023707A (ko) 1995-08-18
KR970010566B1 KR970010566B1 (ko) 1997-06-28

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KR1019940000323A KR970010566B1 (ko) 1994-01-10 1994-01-10 5'-이노신산(5'-lnosinic Acid)을 생산하는 미생물

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