KR940018012A - 체성 배발생 방법 - Google Patents

체성 배발생 방법 Download PDF

Info

Publication number
KR940018012A
KR940018012A KR1019940000563A KR19940000563A KR940018012A KR 940018012 A KR940018012 A KR 940018012A KR 1019940000563 A KR1019940000563 A KR 1019940000563A KR 19940000563 A KR19940000563 A KR 19940000563A KR 940018012 A KR940018012 A KR 940018012A
Authority
KR
South Korea
Prior art keywords
pems
somatic embryos
somatic
iii
suspension culture
Prior art date
Application number
KR1019940000563A
Other languages
English (en)
Other versions
KR100314969B1 (ko
Inventor
잔 반 홀스트 게리트
크레우거 마크
반 데르 메르 비르트
포스트마 에릭
아베스테 롭
Original Assignee
왈터 스몰더스
에스앤드쥐시즈비. 브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27450981&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR940018012(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GB939300706A external-priority patent/GB9300706D0/en
Priority claimed from GB939300705A external-priority patent/GB9300705D0/en
Priority claimed from GB939300707A external-priority patent/GB9300707D0/en
Priority claimed from GB939300729A external-priority patent/GB9300729D0/en
Application filed by 왈터 스몰더스, 에스앤드쥐시즈비. 브이. filed Critical 왈터 스몰더스
Publication of KR940018012A publication Critical patent/KR940018012A/ko
Application granted granted Critical
Publication of KR100314969B1 publication Critical patent/KR100314969B1/ko

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Luminescent Compositions (AREA)
  • External Artificial Organs (AREA)

Abstract

본 발명은 체성 배아 현탁 배양물과 체성 배아를 포함하는 전-배발생물 현탁 배양물(PEMs)에 관한 것으로 PEMs는 거의 유사한 배수도를 갖는 것이며, 이들로부터 식물이 유도된다. 또한 본 발명은 상술한 체성 배아를 수득하는 방법에 관한 것이다.

Description

체성 배발생 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 임펠러 또는 진동 혼합기의 사용과 PEMs(수)/ℓ의 관계를 도시한 것이다.

Claims (20)

  1. 전-배발생물(pro-embryogenic mass;PEM) 형성을 유발하는데 충분한 효과적인 양의 오옥신 또는 오옥신 혼합물을 포함하는 액체 식물조직 배양배지와 비-켈러스 이식(explant) 물질을 접촉시키는 것을 특징으로 하는 상기 이식 물질로부터 생존성을 지닌 체성 배아(somatic embryos)를 산출할 수 있는 PEMs 형성을 촉진시키는 방법.
  2. 하기 단계 ⅰ) 내지 ⅳ)를 포함하는 현탁 배양물에서 거의 같은 배수도의 체성 배아를 수득하는 방법; ⅰ)전-배발생물(PEM) 형성의 유발을 촉진하는데 충분한 효과적인 양의 오옥신 또는 오옥신 혼합물을 포함하는 액체 배지와 접촉된 상태로 이식물질을 배양하는 단계 ; ⅱ)액체 배지를 포함하는 적당한 오옥신과 접촉하여 상기 단계 ⅰ)에서 수득된 PEMs의 수를 중식시키는 단계 ; ⅲ)상기 단계 ⅱ)에서 수득된 PEMs를 수집하고 및 거의 오옥신 부재의 액체 배지에 PEMs를 접촉시키는 단계 ; 및 ⅳ)상기 단계 ⅲ)에서 발생된 PEMs에서 유도된 체성 배아를 수집하는 단계.
  3. 제 2 항에 있어서, 생존성 있는 PEMs가 풍부한 선택된 크기의 PEMs 분획을 상기 단계 ⅲ)에 사용하는 것을 포함하는 방법.
  4. 제 1 항 내지 제 3 항중 어느 한 항에 있어서, 상기 액체 배지가 약 5g/1 내지 90g/1 농도의 탄수화물 에너지 제공원을 추가로 포함하는 것을 특징으로 하는 방법.
  5. 제 4 항에 있어서, 상기 탄수화물 에너지 제공원 농도가 약 20g/1 내지 60g/1 범위에 있는 것을 특징으로 하는 방법.
  6. 제 4 항 또는 제 5 항에 있어서, 상기 탄수화물 에너지 제공원이 수크로오스, 글루코오스 및 라피노스군으로부터 선택되는 것을 특징으로 하는 방법.
  7. 제 1 항 내지 제 6 항중 어느 한 항에 있어서, 상기 오옥신 함유 PEM 현탁물을 진동 혼합기내에서 진동시키는 것을 특징으로 하는 방법.
  8. 제 7 항에 있어서, 상기 진동 혼합기가 거의 수직 평면에서 진동되는 것을 특징으로 하는 방법.
  9. 제 1 항 내지 제 8 항중 어느 한 항에 있어서, 상기 이식물질이 원형질체, 줄기, 잎, 꽃잎, 하배축 단면, 정단 분열 조직, 접합성 배아 자체, 괴경, 유관속, 내초, 자방 또는 꽃밥 필라멘트에서 유도되는 것을 특징으로 하는 방법.
  10. 거의 모두 같은 배수도를 갖는 체성 배아를 포함하는 비-다우커스 식물물질에서 유래한 체성 배아 현탁 배양물.
  11. 제10항에 있어서, 주로 이배체 체성 배아를 포함하는 것을 특징으로 하는 체성 배아 현탁 배양물.
  12. 제10항에 있어서, 주로 사배체 체성 배아를 포함하는 것을 특징으로 하는 체성 배아 현탁 배양물.
  13. 제10항 내지 제12항중 어느 한 항에 있어서, 상기 체성 배아가 쌍자엽 또는 단자엽 이식 물질에서 발생된 PEMs로부터 유도되는 것을 특징으로 하는 현탁 배양물.
  14. 제10항 내지 제13항중 어느 한 항에 있어서, 상기 체성 배아가 시클라멘, 호리병박, 라이코페르시콘, 알리엄, 베고니아, 베타스, 앵초속 식물, 브라시카스, 고추, 시코륨, 게베라, 임페이션, 락투카스, 오리자스, 펠라고늄, 페투니아, 비올라, 및 제아스로 이루어진 군으로부터 선택되는 것을 특징으로 하는 현탁 배양물.
  15. 제14항에 있어서, 쌍자엽 이식물질인 것을 특징으로 하는 현탁 배양물.
  16. 제15항에 있어서, 시클라멘, 호리병박, 베타스, 브라시카스, 비올라, 펠라고늄, 및 고추로 이루어진 군으로부터 선택된 것을 특징으로 하는 현탁 배양물.
  17. 거의 이배체인 세포로 이루어진 PEMs를 포함하는 현탁 배양물.
  18. 거의 모두 같은 배수도를 갖는 비-다우커스 체성 배아.
  19. 제18항에 있어서, 거의 모두 이배체 체성 배아 또는 거의 모두 사배체 체성 배아를 포함하는 것을 특징으로 하는 체성 배아.
  20. 제 2 항 내지 제19항에 의한 체성 배아에서 유래한 식물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940000563A 1993-01-15 1994-01-14 체세포배형성법 KR100314969B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB9300705.2 1993-01-15
GB939300706A GB9300706D0 (en) 1993-01-15 1993-01-15 Improvements in or relating to organic compounds
GB939300705A GB9300705D0 (en) 1993-01-15 1993-01-15 Improvements in or relating to organic compounds
GB9300707.8 1993-01-15
GB9300729.2 1993-01-15
GB939300707A GB9300707D0 (en) 1993-01-15 1993-01-15 Improvements in or relating to organic compounds
GB939300729A GB9300729D0 (en) 1993-01-15 1993-01-15 Improvements in or relating to organic compounds
GB9300706.0 1993-01-15

Publications (2)

Publication Number Publication Date
KR940018012A true KR940018012A (ko) 1994-08-16
KR100314969B1 KR100314969B1 (ko) 2002-02-19

Family

ID=27450981

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940000563A KR100314969B1 (ko) 1993-01-15 1994-01-14 체세포배형성법

Country Status (18)

Country Link
US (1) US5587312A (ko)
EP (2) EP0608716B2 (ko)
JP (1) JP4121160B2 (ko)
KR (1) KR100314969B1 (ko)
CN (1) CN1053468C (ko)
AT (1) ATE187865T1 (ko)
AU (1) AU675094B2 (ko)
CA (1) CA2113374C (ko)
DE (1) DE69422205T3 (ko)
DK (1) DK0608716T4 (ko)
ES (1) ES2140471T5 (ko)
GR (1) GR3032584T3 (ko)
IL (1) IL108330A (ko)
MA (1) MA23089A1 (ko)
PT (1) PT608716E (ko)
TR (1) TR28634A (ko)
TW (1) TW367365B (ko)
ZA (1) ZA94289B (ko)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6164973A (en) * 1995-01-20 2000-12-26 Vincent J. Macri Processing system method to provide users with user controllable image for use in interactive simulated physical movements
GB9703628D0 (en) * 1997-02-21 1997-04-09 Abdelrahman Layla Z Sugar cane production
NZ508752A (en) * 1998-06-12 2003-10-31 Silvagen Inc A process for ex vitro sowing and germination of plant somatic embryos in non-sterile environmentally controlled conditions in a three phase substrate
US6946295B2 (en) 1998-06-12 2005-09-20 Cellfor, Inc. Process for ex vitro sowing and germination of plant somatic embryos
US6265217B1 (en) * 2000-03-01 2001-07-24 Tong Yang Moolsan Company Limited Method for producing microbulbs of garlic {Allium sativum l.} in vitro
US7598073B2 (en) 2002-04-09 2009-10-06 Weyerhaeuser Nr Company Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
US7381562B2 (en) 2002-05-30 2008-06-03 Weyerhaeuser Company Methods for producing cotyledonary pine embryos utilizing a gibberellin
CA2435337C (en) 2002-11-14 2010-03-23 Weyerhaeuser Company Methods for producing conifer somatic embryos
US7521237B2 (en) 2003-06-23 2009-04-21 Weyerhaeuser Nr Company Methods for promoting maturation of conifer somatic embryos
US20040266002A1 (en) * 2003-06-30 2004-12-30 Weyerhaeuser Company Use of abscisic acid in somatic embryogenesis of pine trees
US7732205B2 (en) 2003-07-30 2010-06-08 Weyerhaeuser Nr Company Development and stratification of pine somatic embryos using a liquid system
US7520089B2 (en) 2003-11-25 2009-04-21 Weyerhaeuser Nr Company Method to improve manufactured seed germination
CA2495650C (en) 2004-02-25 2011-05-24 Weyerhaeuser Company Continuous culture of conifer embryogenic tissue
CA2496880C (en) 2004-03-02 2011-01-18 Weyerhaeuser Company Methods for developing conifer somatic embryos
CA2568476C (en) 2005-12-22 2015-03-17 Weyerhaeuser Company Methods for storing conifer somatic embryo germinants
US7858371B2 (en) * 2007-09-26 2010-12-28 Weyerhaeuser Nr Company Method of separating embryo suspension mass
EP2132978A1 (en) * 2008-06-13 2009-12-16 Syngeta Participations AG Method for the production of somatic embryos from Euphorbia pulcherrima plant tissue
CN102763598B (zh) * 2012-08-16 2013-09-11 云南省农业科学院花卉研究所 利用体细胞胚繁育文山兜兰苗的方法
CN105104183B (zh) * 2015-08-26 2017-07-04 北京农业生物技术研究中心 一种菊苣原生质体诱导纯合四倍体植物的方法
US10325215B2 (en) * 2016-04-08 2019-06-18 Pearson Education, Inc. System and method for automatic content aggregation generation
CN107164303B (zh) * 2017-07-19 2020-11-20 泗县微腾知识产权运营有限公司 一种南瓜游离小孢子诱导培养基及诱导培养方法
ES2914629B2 (es) * 2020-12-14 2023-03-06 Instituto Madrileno De Investig Y Desarrollo Rural Agrario Y Alimentario Imidra Procedimiento para la obtencion de biomasa y/o sustancias de valor utilizables como aditivos para piensos a partir de cultivos embriogenicos de encina o alcornoque y producto obtenido.
CN114847159B (zh) * 2022-04-28 2023-04-07 云南龙藏生物科技有限公司 一种川贝母鳞茎组织培养再生方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024944A (en) * 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5030572A (en) * 1987-04-01 1991-07-09 Lubrizol Genetics, Inc. Sunflower regeneration from cotyledons
EP0317512B1 (de) * 1987-11-18 1992-08-05 Ciba-Geigy Ag Eine leistungsfähige Methode, Baumwolle aus kultivierten Zellen zu regenerieren
EP0328424A3 (en) * 1988-02-12 1992-04-29 Kyowa Hakko Kogyo Kabushiki Kaisha Process for the production of somatic embryos
US5236841A (en) * 1989-03-09 1993-08-17 Gupta Pramod K Method for reproducing conifers by somatic embryogenesis using stepwise hormone adjustment
US5482857A (en) * 1989-03-09 1996-01-09 Weyerhaeuser Company Method for reproducing douglas-fir by somatic embryogenesis
US4957866A (en) * 1989-03-09 1990-09-18 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis
ES2084706T3 (es) * 1989-09-30 1996-05-16 Kirin Brewery Metodo de produccion de plantones.
ATE129745T1 (de) * 1990-03-02 1995-11-15 Biocem Regenerierung und genetische transformation der zuckerrübe.
US5334530A (en) * 1992-03-10 1994-08-02 Woods Susan H Method and media for the somatic embryogenesis and regeneration of bamboo
NZ252209A (en) * 1992-05-12 1995-09-26 Moet & Chandon Stabilised culture of proembryogenic cellular aggregates for use in vine regeneration techniques
US5294594A (en) * 1992-12-23 1994-03-15 American Cyanamid Company Water dispersible granular herbicidal compositions comprising dinitroaniline herbicides, montmorillonite carrier and water-swellable polymer
US5491090A (en) * 1994-02-09 1996-02-13 Westvaco Corporation Embryogenic coniferous liquid suspension cultures

Also Published As

Publication number Publication date
TW367365B (en) 1999-08-21
JP4121160B2 (ja) 2008-07-23
JPH06237659A (ja) 1994-08-30
US5587312A (en) 1996-12-24
EP0934691A2 (en) 1999-08-11
IL108330A0 (en) 1994-04-12
DK0608716T4 (da) 2003-11-24
ATE187865T1 (de) 2000-01-15
CN1053468C (zh) 2000-06-14
CN1092107A (zh) 1994-09-14
PT608716E (pt) 2000-05-31
EP0608716A3 (en) 1994-12-07
MA23089A1 (fr) 1994-01-10
DE69422205D1 (de) 2000-01-27
EP0608716B2 (en) 2003-08-06
GR3032584T3 (en) 2000-05-31
AU675094B2 (en) 1997-01-23
DE69422205T3 (de) 2004-04-29
CA2113374C (en) 2008-04-29
CA2113374A1 (en) 1994-07-16
ES2140471T5 (es) 2004-06-01
DE69422205T2 (de) 2000-09-07
ES2140471T3 (es) 2000-03-01
TR28634A (tr) 1996-12-04
DK0608716T3 (da) 2000-06-13
EP0608716A2 (en) 1994-08-03
ZA94289B (en) 1995-07-14
AU5314294A (en) 1994-07-21
EP0608716B1 (en) 1999-12-22
EP0934691A3 (en) 2001-03-28
KR100314969B1 (ko) 2002-02-19
IL108330A (en) 2004-02-19

Similar Documents

Publication Publication Date Title
KR940018012A (ko) 체성 배발생 방법
Muralidharan et al. Plantlet production through high frequency somatic embryogenesis in long term cultures of Eucalyptus citriodora
Ziv et al. Enhanced embryogenesis and plant regeneration from cucumber (Cucumis sativus L.) callus by activated charcoal in solid/liquid double-layer cultures
CA1268631C (en) METHOD FOR THE REGENERATION OF SOYBEAN FROM COTYLEDONAL NODE CULTURES
EP0256751A3 (en) Transformation, somatic embryogenesis and whole plant regeneration method for glycine species
ATE32487T1 (de) Verfahren zur vegetativen vermehrung von pflanzen und analog fuer einen natuerlichen botanischen samen zur ausfuehrung des verfahrens.
Goussard et al. The effectiveness of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines
Krikorian et al. Plantlet production from morphogenetically competent cell suspensions of daylily
Gosch et al. Isolation, culture, and induction of embryogenesis in protoplasts from cell-suspensions of Atropa belladonna
BR8806136A (pt) Metodo para regeneracao de uma planta algodoeira a partir de celulas somaticas
YU48600B (sh) Postupak za genski transfer kod biljaka
Bidney et al. Regeneration of plants from mesophyll protoplasts of Brassica oleracea
Bergervoet et al. Organogenesis versus embryogenesis from long-term suspension cultures of cucumber (Cucumis sativus L.)
Read et al. Accelerating acclimation of in vitro propagated woody ornamentals
Xu et al. Somatic embryogenesis and plant regeneration in cultured immature inflorescences of Setaria italica
Dunstan et al. In vitro studies on organogenesis and growth in Allium cepa tissue cultures
RU2002111666A (ru) Способ получения белковых веществ
Rajoelina et al. Continuous plant regeneration from established embryogenic cell suspension cultures of Italian ryegrass and tall fescue
EP0328424A3 (en) Process for the production of somatic embryos
Dradi et al. In vitro mass propagation of eleven Prunus mahaleb ecotypes
Gould Chromosomal and phenotypic stability during regeneration of whole plants from tissue cultures of Brachycome dichromosomatica (2n= 4)
Dhanalakshmi et al. In vitro somatic embryogenesis and plant regeneration in Clitoria ternatea
Cocking et al. The isolation of protoplasts
Arockiasamy et al. High regenerative nature of Paspalum scrobiculatum L., an important millet crop
JPH07107871A (ja) ラン科植物の培養方法

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20081024

Year of fee payment: 8

LAPS Lapse due to unpaid annual fee