KR930000953A - 생물학적 샘풀중의 단백질 지시제로서의 메로시아닌 및 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인 - Google Patents

생물학적 샘풀중의 단백질 지시제로서의 메로시아닌 및 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인 Download PDF

Info

Publication number
KR930000953A
KR930000953A KR1019920009663A KR920009663A KR930000953A KR 930000953 A KR930000953 A KR 930000953A KR 1019920009663 A KR1019920009663 A KR 1019920009663A KR 920009663 A KR920009663 A KR 920009663A KR 930000953 A KR930000953 A KR 930000953A
Authority
KR
South Korea
Prior art keywords
error indicator
protein
protein error
merocyanine
test strip
Prior art date
Application number
KR1019920009663A
Other languages
English (en)
Other versions
KR100231078B1 (ko
Inventor
에프. 코레이 폴
에이. 마이클즈 안젤라
지. 서머 로날드
Original Assignee
프랭클린 이. 브레켄리지
마일즈 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 프랭클린 이. 브레켄리지, 마일즈 인코포레이티드 filed Critical 프랭클린 이. 브레켄리지
Publication of KR930000953A publication Critical patent/KR930000953A/ko
Application granted granted Critical
Publication of KR100231078B1 publication Critical patent/KR100231078B1/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D263/00—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings
    • C07D263/52—Heterocyclic compounds containing 1,3-oxazole or hydrogenated 1,3-oxazole rings condensed with carbocyclic rings or ring systems
    • C07D263/54—Benzoxazoles; Hydrogenated benzoxazoles
    • C07D263/56—Benzoxazoles; Hydrogenated benzoxazoles with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/12—Radicals substituted by oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D293/00—Heterocyclic compounds containing rings having nitrogen and selenium or nitrogen and tellurium, with or without oxygen or sulfur atoms, as the ring hetero atoms
    • C07D293/10—Heterocyclic compounds containing rings having nitrogen and selenium or nitrogen and tellurium, with or without oxygen or sulfur atoms, as the ring hetero atoms condensed with carbocyclic rings or ring systems
    • C07D293/12—Selenazoles; Hydrogenated selenazoles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D327/00—Heterocyclic compounds containing rings having oxygen and sulfur atoms as the only ring hetero atoms
    • C07D327/02—Heterocyclic compounds containing rings having oxygen and sulfur atoms as the only ring hetero atoms one oxygen atom and one sulfur atom
    • C07D327/04—Five-membered rings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Indole Compounds (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Heterocyclic Compounds Containing Sulfur Atoms (AREA)

Abstract

내용 없음

Description

생물학적 샘플중의 단백질 지시제로서의 메로시아닌 및 니토로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 니트로소 치환된 페놀설폰프탈레인 단백오차 지시제를 합성하기 위한 공정의 도식이고,
제2도는 니트로 치환된 페놀설폰프탈레인 단백오차 지시제를 합성하기 위한 공정의 도식이고,
제3도는 메로시아닌 단백오차 지시제를 합성하기 위한 공정의 도식이다.

Claims (10)

  1. 페놀설폰프탈레인 단백오차 지시제 및 메로시아닌 단백오차 지시제로 함침된 흡수성 지지체를 포함함을 특징으로 하는, 생물학적 샘플중의 단백질 검출용 분석 시험 스트립.
  2. 페놀설폰프탈레인 단백오차 지시제가 하기 일반식(Ⅰ)인 화합물이고 메로시아닌 단백오차 지시제는 하기 일반식(Ⅱ)인 화합물인, 페놀설폰프탈레인 단백오차 지시제 및 메로시아닌 단백오차 지시제로 함침된 흡수성 지지체를 포함함을 특징으로 하는, 생물학적 샘플중의 단백질 검출용 분석 시험 스트립.
    상기식에서, X는 -I, -Br, 또는 -CI이고, Y 는-NO2,또는 -NO이며, Z는 -I, -Br,또는 -CI이고, m은 1내지 6이고, Q는 -Br,-I, 또는 -CI이며, T는 -SO3 e또는 -H이며, R은 S, Se, O 또는 C(Cn2n1)2(여기서, n은 1내지 6이다)
  3. 제2항에 있어서, X가 -Br, 또는 -I이고, Y가 -NO2이며, Z은 -Br이고, m은 3 또는 4이며,Q는 -Br또는-I, 이고 , R이 C(CηHη1)2(여기서, n은 1내지 3이다)이며, T는 -SO3 e인 분석 시험 스트립.
  4. 제2항에 있어서, X가 -Br이고, Y가 -NO2이며, Z은 -Br이고,Q가 -I이며, m은 3이고, R이 (CH3)2분석 시험 스트립.
  5. 제2항에 있어서, 시험 스트립중에 페놀설폰프탈레인 단백오차 지시제와 메로시아닌 단백오차 지시제가 약 10대 1 내지 약 1 대 1의 몰 비율로 함유된 분석 시험 스트립.
  6. 제2항에 있어서, 시험 스트립중에 페놀설폰프탈레인 단백오차 지시제와 메로시아닌 단백오차 지시제가 약 5대 1 내지 약 2대 1의 몰 비율로 함유된 분석 시험 스트립.
  7. 페놀설폰프탈레인 단백오차 지시제가 하기 일반식 (Ⅰ')인 화합물이고 메로시아닌 단백오차 지시제는 하기 일반식(Ⅱ)인 화합물인, 페놀설폰프탈레인 단백오차 지시제 및 메로시아닌 단백오차 지시제로 함침된 흡수성 지지체를 포함함을 특징으로 하는 생물학적 샘플중의 단백질 검출용 분석 시험 스트립.
    상기식에서, X는 -I, -Br, 또는 -CI이고, Y'는 -NO2,또는 -NO이며, Y"는 -I, -Br, 또는 -CI이고, Z는 -I, -Br, 또는 -CI이며, m은 1 내지 6이고, Q는 -Br,-I, 또는 -CI이며, T는 -SO3 e또는 -H이고, R은 S, Se, O 또는 C(CηH2η+1)2(여기서, n은 1내지 6이다)
  8. 제7항에 있어서, 시험 스트립중에 페놀설폰프탈레인 단백오차 지시제와 메로시아닌 단백오차 지시제가 약 10 대 1 내지 약 1 대 1의 몰 비율로 함유된 분석 시트립.
  9. (a) 페놀설폰프탈레인 단백오차 지시제가 하기 일반식(Ⅰ)인 화합물이고 메로시아닌 단백오차 지시제가 하기 일반식(Ⅱ)인 화합물질인, 페놀설폰프탈레인 단백오차 지시제 및 메로시아닌 단백오차 지시제로 함침된 흡수성 지지체를 포함하는 분석 시험 스트립을 생물학적 샘플로 적시고, b) 색 변화가 생물학적 샘플중의 단백질을 나타내는 시험 스트립의 색 변화를 관찰하고 기록하는 단계를 포함함을 특징으로 하여, 생물학적 샘플중의 단백질을 검출하는 방법.
    상기식에서, X는 -I, -Br, 또는 -CI이고, Y 는-NO2,또는 -NO이며, Z는 -I, -Br,또는 -CI이고, m은 1내지 6이고, Q는 -Br,-I, 또는 -CI이며, T는 -SO3 e또는 -H이며, R은 S, Se, O 또는 C(CηH2η+1)2(여기서, n은 1내지 6이다)
  10. a) 페놀설폰프탈레인 단백오차 지시제가 하기 일반식(Ⅰ')인 화합물이고 메로시아닌 단백오차 지시제가 하기 일반식(Ⅱ)인 화합물질인, 페놀설폰프탈레인 단백오차 지시제 및 메로시아닌 단백오차 지시제로 함침된 흡수성 지지체를 포함하는 분석 시험 스트립을 생물학적 샘플로 적시고, b) 색 변화가 생물학적 샘플중의 단백질을 나타내는 시험 스트립의 색 변화를 관찰하고 기록하는 단계를 포함함을 특징으로 하여, 생물학적 샘플중의 단백질을 검출하는 방법.
    상기식에서, X는 -I, -Br, 또는 -CI이고, Y'는 -NO2,또는 -NO이며, Y˝는 -I, -Br, 또는 -CI이고, Z는 -I, -Br, 또는 -CI이며, m은 1 내지 6이고, Q는 -Br,-I, 또는 -CI이며, T는 -SO3 e또는 -H이고, R은 S, Se, O 또는 C(CηH2η+1)2(여기서, n은 1내지 6이다) 이다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임
KR1019920009663A 1991-06-06 1992-06-04 단백질 검출용 분석 시험 스트립 및 이를 사용하는 단백질 검출방법 KR100231078B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US71095391A 1991-06-06 1991-06-06
US07/710,953 1991-06-06
US7/710,953 1991-06-06

Publications (2)

Publication Number Publication Date
KR930000953A true KR930000953A (ko) 1993-01-16
KR100231078B1 KR100231078B1 (ko) 1999-11-15

Family

ID=24856188

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019920009663A KR100231078B1 (ko) 1991-06-06 1992-06-04 단백질 검출용 분석 시험 스트립 및 이를 사용하는 단백질 검출방법

Country Status (12)

Country Link
US (1) US5279790A (ko)
EP (1) EP0517050B1 (ko)
JP (1) JP3224419B2 (ko)
KR (1) KR100231078B1 (ko)
AT (1) ATE143144T1 (ko)
AU (1) AU637017B2 (ko)
CA (1) CA2070040A1 (ko)
DE (1) DE69213826T2 (ko)
DK (1) DK0517050T3 (ko)
ES (1) ES2092595T3 (ko)
GR (1) GR3021150T3 (ko)
TW (1) TW227043B (ko)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5326707A (en) * 1991-11-29 1994-07-05 Miles Inc. Composition and device for urinary protein assay and method of using the same
US5424215A (en) * 1994-02-07 1995-06-13 Miles Inc. Assay for the determination of protein in a biological sample
US5593895A (en) * 1995-04-27 1997-01-14 Bayer Corporation Method for the detection of protein in urine
US5616502A (en) * 1995-05-19 1997-04-01 Molecular Probes, Inc. Non-specific protein staining using merocyanine dyes
US5716851A (en) 1996-01-16 1998-02-10 Bayer Corporation Glass/cellulose as protein reagent
US5753455A (en) * 1996-09-03 1998-05-19 Bayer Corporation Method for the detection of lysozyme using a protein error indicator dye in conjunction with an alkane sulfonic acid
US5908787A (en) * 1997-09-23 1999-06-01 Bayer Corporation Total protein detection method
ES2305255T3 (es) * 2001-06-25 2008-11-01 Siemens Healthcare Diagnostics Inc. Metodos y dispositivos para la deteccion de proteinas totales a ph bajo.
US7459127B2 (en) * 2002-02-26 2008-12-02 Siemens Healthcare Diagnostics Inc. Method and apparatus for precise transfer and manipulation of fluids by centrifugal and/or capillary forces
JP4818586B2 (ja) * 2002-03-05 2011-11-16 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド アミン塩複合体の形成による有機試薬の診断試験装置への吸収
US7125711B2 (en) * 2002-12-19 2006-10-24 Bayer Healthcare Llc Method and apparatus for splitting of specimens into multiple channels of a microfluidic device
US7094354B2 (en) * 2002-12-19 2006-08-22 Bayer Healthcare Llc Method and apparatus for separation of particles in a microfluidic device
US7435381B2 (en) * 2003-05-29 2008-10-14 Siemens Healthcare Diagnostics Inc. Packaging of microfluidic devices
US20040265172A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method and apparatus for entry and storage of specimens into a microfluidic device
US20040265171A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method for uniform application of fluid into a reactive reagent area
US20080257754A1 (en) * 2003-06-27 2008-10-23 Pugia Michael J Method and apparatus for entry of specimens into a microfluidic device
US7347617B2 (en) * 2003-08-19 2008-03-25 Siemens Healthcare Diagnostics Inc. Mixing in microfluidic devices
DE10346863A1 (de) * 2003-10-09 2005-05-04 Roche Diagnostics Gmbh On-Board-Kontrolle für Analyseelemente
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US7399608B2 (en) 2003-12-16 2008-07-15 Kimberly-Clark Worldwide, Inc. Microbial detection and quantification
US7282349B2 (en) 2003-12-16 2007-10-16 Kimberly-Clark Worldwide, Inc. Solvatochromatic bacterial detection
US7300770B2 (en) 2004-12-16 2007-11-27 Kimberly-Clark Worldwide, Inc. Detection of microbe contamination on elastomeric articles
US20060084175A1 (en) * 2004-10-19 2006-04-20 Wayne Comper Colorimetric strip containing coomassie blue for semi-quantitation of albumin
WO2006124816A1 (en) 2005-05-11 2006-11-23 Molecular Probes, Inc. Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use
USRE47452E1 (en) 2007-07-20 2019-06-25 Baxter International Inc. Antimicrobial housing and cover for a medical device
US9125973B2 (en) 2007-07-20 2015-09-08 Baxter International Inc. Antimicrobial housing and cover for a medical device
CN102964863B (zh) * 2010-04-10 2014-04-16 大连理工大学 吲哚类半菁染料的合成与应用
CN101805526A (zh) * 2010-04-10 2010-08-18 大连理工大学 吲哚类半菁染料的合成与应用
US9933359B2 (en) * 2014-06-23 2018-04-03 Xerox Corporation Vendor exclusivity security feature for paper-based diagnostic solution
CN106349146A (zh) * 2016-08-11 2017-01-25 潘忠宁 一种新型光致变色材料的合成方法
CN106957247B (zh) * 2017-03-16 2020-03-20 长春万成生物电子工程有限公司 一种微蛋白指示剂的纯化方法
CN111004166A (zh) * 2019-12-27 2020-04-14 苏州络森生物科技有限公司 一种spdib的制备方法
CN116715977A (zh) * 2023-06-12 2023-09-08 沈阳师范大学 一种半菁染料的制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2510633C3 (de) * 1975-03-12 1978-07-13 Boehringer Mannheim Gmbh, 6800 Mannheim Diagnostisches Mittel zum Nachweis von Eiweiß in Körperflüssigkeiten und dafür geeignete Indikatorfarbstoffe
JPS5767860A (en) * 1980-10-15 1982-04-24 Fuji Photo Film Co Ltd Material for multilayer analysis
US5049358A (en) * 1988-09-30 1991-09-17 Miles Inc. Composition and test device for assaying for proteins
US5122602A (en) * 1989-02-13 1992-06-16 Miles Inc. Chromogenic merocyanine enzyme substrates
US5264589A (en) * 1991-06-06 1993-11-23 Miles Inc. Merocyanine protein error indicators

Also Published As

Publication number Publication date
AU1721092A (en) 1992-12-17
ES2092595T3 (es) 1996-12-01
EP0517050B1 (en) 1996-09-18
TW227043B (ko) 1994-07-21
KR100231078B1 (ko) 1999-11-15
DE69213826T2 (de) 1997-01-30
EP0517050A3 (en) 1993-03-03
JPH05232119A (ja) 1993-09-07
DK0517050T3 (ko) 1997-03-03
US5279790A (en) 1994-01-18
AU637017B2 (en) 1993-05-13
CA2070040A1 (en) 1992-12-07
GR3021150T3 (en) 1996-12-31
EP0517050A2 (en) 1992-12-09
JP3224419B2 (ja) 2001-10-29
ATE143144T1 (de) 1996-10-15
DE69213826D1 (de) 1996-10-24

Similar Documents

Publication Publication Date Title
KR930000953A (ko) 생물학적 샘풀중의 단백질 지시제로서의 메로시아닌 및 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인
DE69320484T2 (de) "up-converting" reporter molekül für biologische und andere testverfahren unter verwendung von laser anregungstecniken
KR930000954A (ko) 메로시아닌 단백오차 지시제
DE68928994T2 (de) Elektrochemilumineszente testverfahren
KR930000498A (ko) 생물 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인
KR890701766A (ko) 균질보호분석법
EP0647849A3 (en) Detection of the presence of analytes in a sample.
DE69013882T2 (de) Zusammensetzung, Vorrichtung und Verfahren zur Untersuchung einer peroxidativ aktiven Substanz.
KR950032639A (ko) 생물학적 샘플중의 단백질을 측정하기 위한 검정법
DE69117588D1 (de) Zusammensetzung und Verfahren zur Bestimmung von Ketonkörpern
DE59309900D1 (de) Verfahren zur Analyse partikelverstärkter Agglutinationsreaktionen auf Zentrifugalanalysatoren durch Bestimmung der Trübungsaufhellung
JPS5849824B2 (ja) アスコルビン酸及びその塩を測定するための組成物および用具
ATE78602T1 (de) Test auf okkultes blut im stuhl.
FI831688A0 (fi) Test foer esterasaktivitet i ett flytande specimen
FI954029A (fi) Kemiluminesoiva reagenssi ja analyysimenetelmä, jossa käytetään substituoitua asetanilidia valon muodostukseen
US4132527A (en) Diagnostic compositions, diagnosing instruments, and methods of manufacturing same
ATE19828T1 (de) Verbessertes fluoreszierendes immunotestsystem.
ES555425A0 (es) Un metodo de medida de la concentracion de un ligando libre en un fluido biologico
DE69231443D1 (de) Reagenzien und verfahren zur quantitativen bestimmung von amitriptylin oder nortriptylin in biologischen flüssigkeiten
DE69430500D1 (de) Chemilumineszierende Derivate mit langer Emissionswellenlänge und ihre Verwendung in Assays
ATE203328T1 (de) Immunchemisches verfahren zum nachweis eines analyten
SE9103427D0 (sv) Foerfarande vid kvantifiering av en substans med hjaelp av fluorescens
GB8811617D0 (en) Assay

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20040823

Year of fee payment: 6

LAPS Lapse due to unpaid annual fee