KR930000498A - 생물 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인 - Google Patents

생물 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인 Download PDF

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KR930000498A
KR930000498A KR1019920009662A KR920009662A KR930000498A KR 930000498 A KR930000498 A KR 930000498A KR 1019920009662 A KR1019920009662 A KR 1019920009662A KR 920009662 A KR920009662 A KR 920009662A KR 930000498 A KR930000498 A KR 930000498A
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compound
biological sample
nitro
formula
test strip
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KR1019920009662A
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KR100232439B1 (ko
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에프 코레이 폴
에이. 마이클즈 안젤라
제이. 프라우드 로이스
살바티 마이클
지. 서머 로날드
더블유. 트리머 로버트
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원본미기재
마일즈 인코포레이티드
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D327/00—Heterocyclic compounds containing rings having oxygen and sulfur atoms as the only ring hetero atoms
    • C07D327/02—Heterocyclic compounds containing rings having oxygen and sulfur atoms as the only ring hetero atoms one oxygen atom and one sulfur atom
    • C07D327/04—Five-membered rings
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Heterocyclic Compounds Containing Sulfur Atoms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Phenolic Resins Or Amino Resins (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

생물 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 니트로소 치환된 단백질 오차 지시제의 합성 방법을 도시한 것이다.
제2도는 니트로 치환된 단백질 오차 지시제의 합성 방법을 도시한 것이다.
제3도는 DIDNTB(5'5'',-디니트로-3'3'',-디요오도-3,4,5,6-테트라브로모페놀설폰프탈레인) 단독(-O-)으로, 및 분자량이 2000인 폴리프로필렌 글리콜과 함께 (-△-) 함침시킨 분석 시험 스트립에 대한 적정 곡선을 도시한 것이다.

Claims (10)

  1. 일반식(I)의 화합물.
    상기식에서, X는 -Cl, Br, 또는 -I이고, Y는 -NO2또는 -NO이며, Z는 -Cl, -Br 또는 -I이다.
  2. 일반식(I-a)의 화합물.
    상기식에서, X는 -I, -Br 또는 -Cl이다.
  3. 구조식(I-b)의 화합물.
  4. 일반식(II)의 화합물.
    상기식에서, X는 -I, -Br 또는 -Cl이고, Y'는 -NO2또는 -NO이며, Y''는 -I, -Br 또는 -Cl이고, Z는 -I, -Br 또는 -Cl이다.
  5. 구조식(II-a)의 화합물.
  6. 일반식(I)의 화합물로 함침시킨 흡수성 담체를 포함하는, 생물 샘플내의 단백질 측정용 분석 시험 스트립.
    상기식에서, X, Y, Z는 제1항에 정의한 바와 같다.
  7. 일반식(II)의 화합물로 함침시킨 흡수성 담체를 포함하는, 생물 샘플내의 단백질 측정용 분석 시험 스트립.
    X, Y', Y", Z는 제4항에 정의한 바와 같다.
  8. (a) 일반식(I)의 화합물로 함침시킨 흡수성 담체를 포함하는 분석 시험 스트립을 생물학적 샘플로 습윤시키고, (b) 생물학적 샘풀내 단백질을 나타내는, 시험 스트립의 모든 색상 변화를 관측 및 기록함을 특징으로 하여, 생물 샘플내의 단백질을 검출하는 방법.
    상기식에서, X, Y 및 Z는 제1항에 정의한 바와 같다.
  9. (a) 일반식(II)의 화합물로 함침시킨 흡수성 담체를 포함하는 분석 시험 스트립을 생물학적 샘플로 습윤시키고, (b) 생물학적 샘풀내 단백질을 나타내는, 시험 스트립의 모든 색상 변화를 관측 및 기록함을 특징으로 하여, 생물 샘플내의 단백질을 검출하는 방법.
    상기식에서, X, Y', Y" 및 Z는 제4항에 정의된 바와 같다.
  10. 제8항 또는 제9항에 있어서, 추가로 시험 스트립을 완충용액 및 색상 강화 중합체로 함침시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920009662A 1991-06-06 1992-06-04 생물학적 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인, 이를 포함하는 시험 스트립 및 이를 사용한 단백질 검출 방법 KR100232439B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/710,952 1991-06-06
US7/710,952 1991-06-06
US07/710,952 US5187104A (en) 1991-06-06 1991-06-06 Nitro or nitroso substituted polyhalogenated phenolsulfonephthaleins as protein indicators in biological samples

Publications (2)

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KR930000498A true KR930000498A (ko) 1993-01-15
KR100232439B1 KR100232439B1 (ko) 1999-12-01

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KR1019920009662A KR100232439B1 (ko) 1991-06-06 1992-06-04 생물학적 샘플내 단백질 지시제로서 니트로 또는 니트로소 치환된 폴리할로겐화 페놀설폰프탈레인, 이를 포함하는 시험 스트립 및 이를 사용한 단백질 검출 방법

Country Status (17)

Country Link
US (1) US5187104A (ko)
EP (1) EP0517054B1 (ko)
JP (1) JP3157607B2 (ko)
KR (1) KR100232439B1 (ko)
AT (1) ATE161838T1 (ko)
AU (1) AU636540B2 (ko)
CA (1) CA2070039A1 (ko)
DE (1) DE69223851T2 (ko)
DK (1) DK0517054T3 (ko)
ES (1) ES2111009T3 (ko)
FI (1) FI922588A (ko)
GR (1) GR3026262T3 (ko)
IE (1) IE921838A1 (ko)
IL (1) IL101859A (ko)
NO (1) NO922071L (ko)
NZ (1) NZ242731A (ko)
ZA (1) ZA923804B (ko)

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US5424215A (en) * 1994-02-07 1995-06-13 Miles Inc. Assay for the determination of protein in a biological sample
US5750405A (en) * 1996-03-01 1998-05-12 Bayer Corporation Method for the detection for protein
KR100352837B1 (ko) * 2001-05-22 2002-09-16 성홍제 호스용 폴리에틸렌 타포린과 그 제조 방법 및 호스용폴리에틸렌 타포린을 사용하여 제조된 호스
US7459127B2 (en) * 2002-02-26 2008-12-02 Siemens Healthcare Diagnostics Inc. Method and apparatus for precise transfer and manipulation of fluids by centrifugal and/or capillary forces
JP3955911B2 (ja) * 2002-08-09 2007-08-08 アークレイ株式会社 尿中のアルブミン測定方法、尿中のアルブミン測定用指示薬、および尿中のアルブミン測定用試験片
CN1322329C (zh) * 2002-08-09 2007-06-20 爱科来株式会社 蛋白质测定用试验片及其制造方法
US7125711B2 (en) * 2002-12-19 2006-10-24 Bayer Healthcare Llc Method and apparatus for splitting of specimens into multiple channels of a microfluidic device
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US20040265172A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method and apparatus for entry and storage of specimens into a microfluidic device
US20040265171A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method for uniform application of fluid into a reactive reagent area
US7347617B2 (en) * 2003-08-19 2008-03-25 Siemens Healthcare Diagnostics Inc. Mixing in microfluidic devices
CN102617410B (zh) * 2012-03-15 2013-11-06 长春万成生物电子工程有限公司 一种3,4,5,6-四卤代酚磺酞的纯化方法
CN106957247B (zh) * 2017-03-16 2020-03-20 长春万成生物电子工程有限公司 一种微蛋白指示剂的纯化方法
KR102268024B1 (ko) 2019-09-30 2021-06-23 주식회사 청도제약 높은 민감도를 가진 소변 내 미세알부민 검출 시험 스트립

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DE2510633C3 (de) * 1975-03-12 1978-07-13 Boehringer Mannheim Gmbh, 6800 Mannheim Diagnostisches Mittel zum Nachweis von Eiweiß in Körperflüssigkeiten und dafür geeignete Indikatorfarbstoffe

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AU636540B2 (en) 1993-04-29
ZA923804B (en) 1993-03-10
KR100232439B1 (ko) 1999-12-01
IL101859A0 (en) 1992-12-30
IE921838A1 (en) 1992-12-16
CA2070039A1 (en) 1992-12-07
NZ242731A (en) 1993-12-23
EP0517054A1 (en) 1992-12-09
IL101859A (en) 1998-09-24
ATE161838T1 (de) 1998-01-15
ES2111009T3 (es) 1998-03-01
EP0517054B1 (en) 1998-01-07
GR3026262T3 (en) 1998-05-29
DE69223851D1 (de) 1998-02-12
NO922071D0 (no) 1992-05-25
FI922588A (fi) 1992-12-07
NO922071L (no) 1992-12-07
JP3157607B2 (ja) 2001-04-16
DE69223851T2 (de) 1998-04-16
US5187104A (en) 1993-02-16
DK0517054T3 (da) 1998-09-07
FI922588A0 (fi) 1992-06-04
JPH05230054A (ja) 1993-09-07
AU1804592A (en) 1992-12-17

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