KR910001042A - 에폭시-활성화된 비이드상에 고정된 폴리뉴클레오티드 포스포릴라제 - Google Patents

에폭시-활성화된 비이드상에 고정된 폴리뉴클레오티드 포스포릴라제 Download PDF

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KR910001042A
KR910001042A KR1019890008296A KR890008296A KR910001042A KR 910001042 A KR910001042 A KR 910001042A KR 1019890008296 A KR1019890008296 A KR 1019890008296A KR 890008296 A KR890008296 A KR 890008296A KR 910001042 A KR910001042 A KR 910001042A
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리차드 모랜 죤
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제임스 제이.플라인
이.아이.듀퐁 드 네모아 앤드 캄파니
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    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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Abstract

내용 없음.

Description

에폭시-활성화된 비이드상에 고정된 폴리뉴클레오티드 포스포릴라제
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 폴리뉴클레오티드 포스포릴라제 (PNP아제) 고정반응에서의 pH의 효과를 나타낸 것이고,
제2도는 폴리뉴클레오티드 생성물의 분자량에 대한 지지체 공극크기 및 반응시간의 효과를 나타낸 것이다.

Claims (19)

  1. 고체의 수불성인, 에폭시-활성화된 유기 중합체의 입자에 공유결합에 의해 고정된 풀리-뉴클레오티드포스포릴라제.
  2. 제1항에 있어서, 중합체가 에폭시-활성화된 다당체, 폴리(메트) 아크릴레이트, 폴리(메트)아크릴 아미드, 폴리비닐아세테이트 또는 폴리비닐알코올인 폴리뉴클레오티드 포스포릴라제.
  3. 제2항에 있어서, 중합체가 옥시란 아크릴성 단일중합체 또는 공중합체이고 입자의 평균직경이 약 1 내지 1,000㎛이며 입자 공극의 평균직경이 약 0.01 내지 1㎛일 폴리뉴클레오티드 포스포릴라제.
  4. 제3항에 있어서, 입자의 평균 직경이 약 10내지 500㎛이고 입자 공극의 평균직경이 약 0.02 내지 0.5㎛인 폴리뉴클레오티드 포스포릴라제.
  5. 제4항에 있어서, 중합체가 (A) 글리시딜 그룹을 지닌 에틸렌성 불포화 단량체, (B) 2개 이상의 라디칼 중합가능한 이중결합을 지닌 공단량체 및 (C) 라디칼-중합가능한 수용성 공단량체의 공중합체인 폴리뉴클레오티드 포스포릴라제.
  6. 제5항에 있어서, 중합체가 (A)글리시딜 메타크릴레이트 및/또는 알릴 글리시딜 에테르, (B) N.N'-메틸렌-비스-메타크릴아미드 및 (C) 메타크릴아미드의 공중합체인 폴리뉴클레오티드 포스포릴라제.
  7. 제6항에 있어서, 입자의 직경이 약 100 내지 300㎛이고 입자 공극의 직경이 약 0.02 내지 0.2㎛인 폴리뉴클레오티드 포스포릴라제.
  8. 제3항에 있어서, 중합체가 에폭시-활성화된 폴리비닐알코올인 폴리뉴클레오티드 포스포릴라제.
  9. 폴리뉴클레오티드 포스포릴라제를 pH 4내지 6범위에서 수용액중에 고체의 수-불용성인, 에폭시-활성화된 유기 중합체와 반응시킴을 특징으로 하여, 폴리뉴클레오티드 포스포릴라제를 고정시키는 방법.
  10. 제9항에 있어서, 반응을 4-모르폴린에탄설폰산 완충액 중에서 수행하는 방법.
  11. 제10항에 있어서, 중합체가 옥시란 아크릴성 또는 에폭시-활성화된 폴리비닐 알코올이며 입자의 평균 직경이 약 100 내지 300㎛이고 입자 공극의 평균 직경이 약 0.02 내지 0.2㎛인 방법.
  12. 고정된 폴리뉴클레오티드 포스포릴라제 존재하에 폴리리보뉴클레오티드를 뉴클레오시드 디포스페이트로 부터 합성하는 방법에 있어서, 고체의 수-불용성인, 에폭시-활성화된 유기중합체의 입자에 공유결합에 의해 고정된 폴리뉴클레오티드 포스포릴라제를 사용함을 특징으로 하는 방법.
  13. 제12항에 있어서, 중합체가 에폭시-활성화된 다당체, 폴리(메트)아크릴레이트, 폴리(메트)아크릴아미드, 폴리비닐아세테이트 또는 폴리비닐 알코올인 방법.
  14. 제13항에 있어서, 중합체가 옥시란 아크릴성 단일중합체 또는 공중합체이고 입자의 평균직경이 약 1 내지 1,000㎛이며 입자 공극의 평균직경이 약 0.01 내지 1㎛인 방법.
  15. 제14항에 있어서, 입자의 평균 직경이 약 10 내지 500㎛이고 입자 공극의 평균직경이 약 0.02 내지 0.5㎛인 방법.
  16. 제15항에 있어서, 중합체가 (A) 글리시딜 그룹을 지닌 에틸렌성 불포화 단량체, (B) 2개 이상의 라디칼 중합가능한 이중결합을 지닌 공단량체 및 (C) 라디칼-중합가능한 수용성 공단량체의 공중합체인 방법.
  17. 제16항에 있어서, 중합체가 (A)글리시딜 메타크릴레이트 및/또는 알릴 글리시딜 에테르, (B) N,N'-메틸렌-비스-메타크릴아미드 및 (C) 메타크릴 아미드의 공중합체인 방법.
  18. 제17항에 있어서, 입자의 직경이 약 100 내지 300㎛이고 입자의 공극 직경이 약 0.02 내지 0.2㎛인 방법.
  19. 고정된 폴리뉴클레오티드 포스포릴라제 존재하에 폴리뉴클레오티드의 탈인산화에 의해 리보뉴클레오시드 디포스페이트를 생성시키는 방법에 있어서, 고체의 수-불용성인, 에폭시-활성화된 유기 중합체의 입자에 공유결합에 의해 고정된 폴리뉴클레오티드 포스포릴라제를 사용함을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890008296A 1988-06-16 1989-06-16 에폭시-활성화된 비이드상에 고정된 폴리뉴클레오티드 포스포릴라제 KR910005628B1 (ko)

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US20819488A 1988-06-16 1988-06-16
US208,194 1988-06-16
US208194 1988-06-16

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EP (1) EP0346865A3 (ko)
JP (1) JPH02109979A (ko)
KR (1) KR910005628B1 (ko)
AU (1) AU3653789A (ko)
DK (1) DK296089A (ko)
FI (1) FI892939A (ko)
HU (1) HUT50865A (ko)
IL (1) IL90600A0 (ko)
NO (1) NO892497L (ko)
PT (1) PT90886A (ko)
ZA (1) ZA894605B (ko)

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Publication number Priority date Publication date Assignee Title
WO1991000346A1 (en) * 1989-07-03 1991-01-10 E.I. Du Pont De Nemours And Company Polynucleotide phosphorylase immobilized on tris(hydroxymethyl)methylacrylamide polymer beads
EP0562373A3 (en) * 1992-03-23 1994-05-25 Siemens Ag Immobilisation of biochemical substances
DE4333674A1 (de) * 1993-10-02 1995-04-06 Merck Patent Gmbh Nukleotidhaltiges Sorbens für die Affinitätschromatographie
DE19653730C2 (de) * 1996-12-11 1999-06-24 Schering Ag Immobilisierte Proteine aus Rohextrakt und deren Verwendung zur Umsetzung von Estern
US5905024A (en) * 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
KR20010101092A (ko) * 1998-12-10 2001-11-14 오미야 히사시 운반체상에 ｄｎａ를 고정화시키는 방법
DE60315790T2 (de) * 2003-01-16 2008-06-05 Adorkem Technology S.P.A., Costa Volpino Immobilisierte Biokatalysatoren für die Herstellung von natürlichen Nukleosiden und modifizierten Analogen durch enzymatische Transglykosylierungsreaktionen
BR112023001947A2 (pt) * 2020-08-05 2023-04-11 Oncovir Inc Produção escalonável de polirribonucleotídeos de tamanho controlado

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US4210723A (en) * 1976-07-23 1980-07-01 The Dow Chemical Company Method of coupling a protein to an epoxylated latex
US4208309A (en) * 1977-05-20 1980-06-17 Rohm Gmbh Pearl polymer containing hollow pearls
DE3136940A1 (de) * 1981-09-17 1983-03-31 Hoechst Ag, 6230 Frankfurt "verfahren zur herstellung von 5'-ribonucleotiden"
US4582860A (en) * 1983-12-15 1986-04-15 Rohm And Haas Company Oxirane resins for enzyme immobilization

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DK296089D0 (da) 1989-06-15
NO892497L (no) 1989-12-18
IL90600A0 (en) 1990-01-18
PT90886A (pt) 1989-12-29
ZA894605B (en) 1991-02-27
JPH02109979A (ja) 1990-04-23
FI892939A (fi) 1989-12-17
FI892939A0 (fi) 1989-06-15
DK296089A (da) 1989-12-17
EP0346865A3 (en) 1990-09-12
KR910005628B1 (ko) 1991-08-01
NO892497D0 (no) 1989-06-15
AU3653789A (en) 1989-12-21
EP0346865A2 (en) 1989-12-20

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