KR900006509A - 인간 조직형 플라스미노겐 액티베이터(plasminogen activator)의 제조방법 - Google Patents

인간 조직형 플라스미노겐 액티베이터(plasminogen activator)의 제조방법 Download PDF

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KR900006509A
KR900006509A KR1019890014230A KR890014230A KR900006509A KR 900006509 A KR900006509 A KR 900006509A KR 1019890014230 A KR1019890014230 A KR 1019890014230A KR 890014230 A KR890014230 A KR 890014230A KR 900006509 A KR900006509 A KR 900006509A
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medium
human tissue
plasminogen activator
tissue plasminogen
acid
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KR1019890014230A
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KR920007398B1 (ko
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쇼이찌로 미야하라
아쓰노리 신도
마끼 스즈끼
노부미 구수하라
노부요시 마끼구찌
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사와무라 하루오
미쓰이 도오아쓰 가가꾸 가부시끼가이샤
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Priority claimed from JP63249081A external-priority patent/JP2648624B2/ja
Priority claimed from JP63316118A external-priority patent/JP2718726B2/ja
Application filed by 사와무라 하루오, 미쓰이 도오아쓰 가가꾸 가부시끼가이샤 filed Critical 사와무라 하루오
Publication of KR900006509A publication Critical patent/KR900006509A/ko
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Publication of KR920007398B1 publication Critical patent/KR920007398B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure

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  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

인간 조직형 플라스미노겐 액티베이터(plasminogen activator)의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (6)

  1. 삼투압이 적어도 350milliosmoles/ℓ까지 비카르보네이트 이온을 사용하여 증가된 세포 배양 배지에서 인간 조직형 플라스마노겐 액티베이터는 세포 성장과 병행하여 생성되는, 세로를 사용하여 인간 조직형 플라스마노겐 액티베이터를 생성하는 방법.
  2. 세포가 배지에서먼저 성장하고 난다음 인간 조직형 플라스미노겐 액티베이터의 생성을 위해 배지에서 배양되고, 비카르보네이트 이온을 사용하여 증가된 인간 조직형 플라스마노겐 액티베이터의 생성을 위해 상술한 배지에서의 삼투압을 적어도 350milliosmoles/ℓ까지 증가시키는, 세포를 사용하여 인간 조직형 플라스미노겐 액티베이터를 생성하는 방법.
  3. 제1 내지 2항에 있어서, 상술된 비카르보네이트 이온이 소디움 비카르보네이트이고 배지중의 그들의 농도가 적어도 3g/ℓ인 방법.
  4. 제1 내지 2항에 있어서, 저분자량 항플라스민제가 또한 상술된 배지에 가해지는 방법.
  5. 제4항에 있어서, 상술된 항 플라스민이 4-아미노 부탄노산, 5-아미노펜타노산, 6-아미노헥사노산, 트랜스-4-(아미노메틸) 시클로헥산카르복실산 및 트랜스-4-(아미노에틸) 시클로헥산카르복실산으로 구성되는 기에서 선택되는 방법.
  6. 제4항에 있어서, 배지의 상술한 항플라스민제의 농도가 10+4내지 10+1M의 범위인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890014230A 1988-10-04 1989-10-04 인간 조직형 플라스미노겐 액티베이터(Plasminogen activator)의 제조방법 KR920007398B1 (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP249081 1988-10-04
JP63249081A JP2648624B2 (ja) 1988-10-04 1988-10-04 ヒト組織型プラスミノーゲン活性化因子の製造方法
JP63-249081 1988-10-04
JP63316118A JP2718726B2 (ja) 1988-12-16 1988-12-16 ヒト組織型プラスミノーゲン活性化因子の製法
JP316118 1988-12-16
JP63-316118 1988-12-16

Publications (2)

Publication Number Publication Date
KR900006509A true KR900006509A (ko) 1990-05-08
KR920007398B1 KR920007398B1 (ko) 1992-08-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890014230A KR920007398B1 (ko) 1988-10-04 1989-10-04 인간 조직형 플라스미노겐 액티베이터(Plasminogen activator)의 제조방법

Country Status (11)

Country Link
US (1) US5183754A (ko)
EP (1) EP0366285B1 (ko)
KR (1) KR920007398B1 (ko)
AT (1) ATE116363T1 (ko)
AU (1) AU614429B2 (ko)
CA (1) CA1335188C (ko)
DE (1) DE68920269T2 (ko)
DK (1) DK488489A (ko)
FI (1) FI96039C (ko)
NO (1) NO175787C (ko)
NZ (1) NZ230856A (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5151359A (en) * 1988-05-19 1992-09-29 Mitsui Toatsu Chemicals Incorporated Method for producing of human tissue type plasminogen activator
CA2829774C (en) 2011-03-14 2019-09-24 National Research Council Of Canada Method of viral production in cells

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3732146A (en) * 1970-12-22 1973-05-08 Behringwerke Ag Process for the isolation of native, highly purified plasminogen
US4766075A (en) * 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
GB8334499D0 (en) * 1983-12-24 1984-02-01 Beecham Group Plc Derivatives
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media
US4661453A (en) * 1984-06-19 1987-04-28 American Biogenetic Sciences, Inc. Production of tissue plasminogen activator factor
JPS624233A (ja) * 1985-07-01 1987-01-10 Toyobo Co Ltd 組織性プラスミノ−ゲン活性化因子の製造法
US4960702A (en) * 1985-09-06 1990-10-02 Codon Methods for recovery of tissue plasminogen activator
CA1312030C (en) * 1987-11-18 1992-12-29 Brian Maiorella Method to increase antibody titer
US5151359A (en) * 1988-05-19 1992-09-29 Mitsui Toatsu Chemicals Incorporated Method for producing of human tissue type plasminogen activator

Also Published As

Publication number Publication date
NO893929L (no) 1990-04-05
ATE116363T1 (de) 1995-01-15
NO893929D0 (no) 1989-10-03
DK488489A (da) 1990-04-05
EP0366285B1 (en) 1994-12-28
NO175787B (no) 1994-08-29
EP0366285A1 (en) 1990-05-02
FI96039B (fi) 1996-01-15
DE68920269T2 (de) 1995-05-18
NZ230856A (en) 1991-10-25
NO175787C (no) 1994-12-07
CA1335188C (en) 1995-04-11
AU4254189A (en) 1990-04-12
FI894657A (fi) 1990-04-05
AU614429B2 (en) 1991-08-29
FI894657A0 (fi) 1989-10-02
DK488489D0 (da) 1989-10-04
KR920007398B1 (ko) 1992-08-31
FI96039C (fi) 1996-04-25
US5183754A (en) 1993-02-02
DE68920269D1 (de) 1995-02-09

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