KR900000380A - 활성적인 형태의 단백질 분리방법 - Google Patents

활성적인 형태의 단백질 분리방법 Download PDF

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KR900000380A
KR900000380A KR1019880007253A KR880007253A KR900000380A KR 900000380 A KR900000380 A KR 900000380A KR 1019880007253 A KR1019880007253 A KR 1019880007253A KR 880007253 A KR880007253 A KR 880007253A KR 900000380 A KR900000380 A KR 900000380A
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protein
cation
cationic
host cell
surfactant
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KR1019880007253A
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KR0126767B1 (ko
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죤 패트로니 죠셉
로이 브랜돈 말콤
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원본미기재
번지(오스트레일리아) 프로프라이어더리 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Chemical & Material Sciences (AREA)
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  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Endocrinology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

알킬 글루코시드의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 단백질 분리방법에 있어서, 합성된 발현된 단백질을 포함하는 숙주 세포의 자원 ; 및 최소한 하나의 양이온성, 음이온성 또는 쌍성이온성 계면활성제의 자원을 제공하고 ; 최소한 하나의 양이온성, 음이온성 또는 쌍성이온성 계면활성제를 원하는 단백질 가용화에 충분한 량으로 상기 숙주 세포에 처리하는 과정을 포함함을 특징으로 하는 단백질 분리방법.
  2. 제1항에 있어서, 합성된 또는 발현된 단백질을 포함하는 숙주 세포를 함유하는 발효액을 제공하고 그로부터 숙주세포를 분리하는 예비 단계를 포함함을 특징으로 하는 단백질 분리방법.
  3. 제2항에 있어서, 전체적인 세포 용해질을 형성시키기 위하여 숙주 세포를 붕괴시키는 동시 형성단계를 포함함을 특징으로 하는 단백질 분리방법.
  4. 제1항에 있어서, 합성된 또는 발현된 단백질은 단량체 또는 다량체의 세포내 단백질을 포함함을 특징으로 하는 분리방법.
  5. 제4항에 있어서, 단량체 또는 다량체의 세포내 단백질은 성장 호르몬, 인터페론, 임뮤노겐, 임파액 또는 그들의 이종 또는 동종 중합체로부터 선택한 봉입체를 포함함을 특징으로 하는 단백질 분리방법.
  6. 제5항에 있어서, 최소한 하나의 양이온성, 음이온성 또는 쌍성이온성 계면활성체는 약 2.5-5.0 W/V%량으로 존재함을 특징으로 하는 단백질 분리방법.
  7. 제6항에 있어서, 상게 계면활성제는 다음 양이온중에서 선택한 양이온을 포함하는 양이온 계면활성제임을 특징으로 하는 단백질 분리방법 ; 세틸 트리메틸암모늄 양이온, 세틸 피리디나움 양이온, 테트라데실 트리메틸암모늄 양이온, 도데실 트리메틸암모늄 양이온, 혼합한 n-알킬 디메틸 벤질 암모늄 양이온,,-디메틸,--[2-[2-[4-(1,1,3,3-테트라메틸부틸)페녹시]에톡시]에틸]벤젠메탄아미니움 양이온, 도데실 디메틸아민 산화물,-라우로알사르코신 나트륨 염,-라우로일--메틸라우린 나트륨, 염,-라우릴-이미노디프로피온산 나트륨 염, 3-(,-디메틸 라우릴암모니오)프로판 술폰산 나트륨 염.
  8. 제7항에 있어서, 양이온성 계면활성제는 세틸 트리 메틸암모늄 브룸화물임을 특징으로 하는 단백질분리방법.
  9. 제8항에 있어서, 최소한 하나의 양이온 계면활성제는 미셀 농도를 초과하는 량으로 존재함을 특징으로 하는 단백질 분리방법.
  10. 제6항에 있어서, 생성된 거친 용액으로부터 가융화된 단백질을 분리시키는 분리단계는 크로마토그래피관, 투석, 한외거르기, 분별결정, 또는 리간드 특수 분리를 통한 가용화된 단백질 분별 용리중에서 선택함을 특징으로 하는 단백질 분리방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880007253A 1987-06-15 1988-06-16 계면활성제를 이용한 단백질 회수방법 KR0126767B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPI247287 1987-06-15
AUPI12472 1987-06-16
AU?PI2472? 1987-06-16

Publications (2)

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KR900000380A true KR900000380A (ko) 1990-01-30
KR0126767B1 KR0126767B1 (ko) 1997-12-29

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Country Status (14)

Country Link
US (1) US4992531A (ko)
JP (1) JPS6447389A (ko)
KR (1) KR0126767B1 (ko)
CN (1) CN1032425C (ko)
AT (1) ATE114156T1 (ko)
AU (1) AU609824B2 (ko)
CA (1) CA1305284C (ko)
DE (1) DE3852116T2 (ko)
DK (1) DK322188A (ko)
ES (1) ES2065911T3 (ko)
IE (1) IE66334B1 (ko)
NO (1) NO882635L (ko)
NZ (1) NZ225032A (ko)
ZA (1) ZA884212B (ko)

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JP3279362B2 (ja) * 1992-10-05 2002-04-30 井上 聰一 糖尿病判定方法及び糖尿病判定装置
JP3319812B2 (ja) * 1993-04-05 2002-09-03 井上 聰一 リウマチ判定方法及びリウマチ判定装置
US20030190307A1 (en) * 1996-12-24 2003-10-09 Biogen, Inc. Stable liquid interferon formulations
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DE69943205D1 (de) 1998-08-06 2011-03-31 Mountain View Pharmaceuticals Peg-uricase Konjugate und Verwendung davon
ATE367398T1 (de) * 2000-05-16 2007-08-15 Bolder Biotechnology Inc Verfahren zur rückfaltung von proteinen mit freien cysteinresten
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CZ2007700A3 (cs) 2005-04-11 2008-02-27 Savient Pharmaceuticals, Inc. Variantní forma urátoxidázy a její použití
WO2006125452A1 (en) * 2005-05-23 2006-11-30 Universite De Geneve Injectable superparamagnetic nanoparticles for treatment by hyperthermia and use for forming an hyperthermic implant
EP2778234B1 (en) 2005-05-31 2017-09-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP2102355B1 (en) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
US8288559B2 (en) * 2008-08-18 2012-10-16 Promega Corporation Luminogenic compounds and methods to detect cytochrome P450 3A enzymes
GB0901434D0 (en) * 2009-01-29 2009-03-11 Univ Strathclyde Ballast water treatment system
KR101861547B1 (ko) 2009-06-25 2018-07-02 크레알타 파마슈티칼스 엘엘씨 페길화된 유리카아제 치료 중에 혈청 요산을 모니터링하여 주입 반응의 위험성 및 반응의 항체­매개 상실을 예측하는 방법 및 키트
CN102905690B (zh) 2010-05-21 2016-03-30 西门子医疗保健诊断公司 两性离子试剂
CN102965317A (zh) * 2012-11-27 2013-03-13 江南大学 一种产牛磺酸菌株的筛选方法及用该菌株发酵法生产牛磺酸
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
CN107075544B (zh) * 2014-07-22 2021-01-29 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液

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KR0126767B1 (ko) 1997-12-29
CN1032425C (zh) 1996-07-31
DK322188A (da) 1988-12-17
US4992531A (en) 1991-02-12
AU1763288A (en) 1988-12-15
DE3852116D1 (de) 1994-12-22
ES2065911T3 (es) 1995-03-01
ATE114156T1 (de) 1994-12-15
DK322188D0 (da) 1988-06-14
IE881815L (en) 1988-12-15
CN88103725A (zh) 1988-12-28
NO882635D0 (no) 1988-06-15
DE3852116T2 (de) 1995-03-30
CA1305284C (en) 1992-07-14
NO882635L (no) 1988-12-19
ZA884212B (en) 1989-03-29
AU609824B2 (en) 1991-05-09
JPS6447389A (en) 1989-02-21
IE66334B1 (en) 1995-12-27
NZ225032A (en) 1991-02-26

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