KR880004090A - 트랜스아미나제 유전자 tyrB의 클로닝 및 용도 - Google Patents

트랜스아미나제 유전자 tyrB의 클로닝 및 용도 Download PDF

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KR880004090A
KR880004090A KR870010362A KR870010362A KR880004090A KR 880004090 A KR880004090 A KR 880004090A KR 870010362 A KR870010362 A KR 870010362A KR 870010362 A KR870010362 A KR 870010362A KR 880004090 A KR880004090 A KR 880004090A
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Prior art keywords
microorganism
coli
extrachromosomal
tyrb
extrachromosomal factor
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KR870010362A
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English (en)
Inventor
마르콰르트 뤼디거
텐 요한
데거 한스-마티아스
뵈너 게르하르트
로빈슨 마틴
도허티 앤드류
Original Assignee
하인리히 벡커, 베른하르트 벡크
훽스트 아크티엔게젤샤프트
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Application filed by 하인리히 벡커, 베른하르트 벡크, 훽스트 아크티엔게젤샤프트 filed Critical 하인리히 벡커, 베른하르트 벡크
Publication of KR880004090A publication Critical patent/KR880004090A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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  • Chemical & Material Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

트랜스아미나제 유전자 tyrB의 클로닝 및 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 이. 콜라이(E.Coli) ATCC 11303으로 부터 분리된 tyrB 유전자를 함유하는 복제가능한 염색체외 인자.
  2. 제1항에 있어서, 다복제 플라스미드인 염색체외 인자.
  3. 제2항에 있어서, 다복제 플라스미드가 pAT 153인 염색체외 인자.
  4. 방향족 트랜스아미나제를 합성하기 위한, 제1항 내지 제3항중 어느 하나에서 청구한 염색체외 인자의 용도.
  5. (a) 염색체외 인자를 미생물 내로 도입시키고, (b) 미생물에서 tyrB를 발현시키고, 활성 방향족 트랜스아미나제를 합성시킨 다음, (c) 페닐피루베이트를 트랜스아미나제에 의해 아미노화 하는 단계로 이루어지는 방법에 의해 미생물내에서 L-페닐알라닌을 과잉생산하기 위한 제1항 내지 제3항중 어느 하나에서 청구한 염색체외 인자의 용도.
  6. 제4항에 있어서, 미생물이 장내세균속인 용도.
  7. 제5항에 있어서, 미생물이 이. 콜라이인 용도.
  8. 제7항에 있어서, 미생물이 이. 콜라이 ATCC 11303 및 이의 변종 및 돌연변이체인 용도.
  9. 제1항 내지 제3항중 어느 하나에서 청구한 염색체외 인자로 형질전환된 이. 콜라이 ATCC 11303 및 이의 변종 돌연변이체.
  10. 이. 콜라이 ATCC 11303으로부터 수득 가능한 tyrB 유전자 및 이의 변종 및 돌연변이체.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR870010362A 1986-09-19 1987-09-18 트랜스아미나제 유전자 tyrB의 클로닝 및 용도 KR880004090A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19863631829 DE3631829A1 (de) 1986-09-19 1986-09-19 Klonierung und verwendung des transaminase gens-tyrb
DEP3631829.9 1986-09-19

Publications (1)

Publication Number Publication Date
KR880004090A true KR880004090A (ko) 1988-06-01

Family

ID=6309879

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Application Number Title Priority Date Filing Date
KR870010362A KR880004090A (ko) 1986-09-19 1987-09-18 트랜스아미나제 유전자 tyrB의 클로닝 및 용도

Country Status (16)

Country Link
US (1) US5091314A (ko)
EP (1) EP0260597B1 (ko)
JP (2) JP2760788B2 (ko)
KR (1) KR880004090A (ko)
CN (1) CN87106923A (ko)
AT (1) ATE92104T1 (ko)
AU (1) AU7866887A (ko)
CA (1) CA1341096C (ko)
DE (2) DE3631829A1 (ko)
DK (1) DK490187A (ko)
FI (1) FI874058A (ko)
IE (1) IE872530L (ko)
IL (1) IL83934A0 (ko)
NO (1) NO873919L (ko)
PT (1) PT85752B (ko)
ZA (1) ZA877031B (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3786707D1 (de) * 1986-06-04 1993-09-02 Hoechst Ag Verfahren zur herstellung von l-phosphinothricin durch transaminierung.
DE3721838A1 (de) * 1987-07-02 1989-01-12 Hoechst Ag Verfahren zur herstellung von l-phenylalanin aus benzylidenhydantoin
US5852030A (en) * 1992-09-29 1998-12-22 Toray Industries, Inc. Indole derivatives, process for producing the same and medicinal uses of the same
CN1043766C (zh) * 1992-09-29 1999-06-23 东丽株式会社 吲哚衍生物及其制法和它们在制备药物中的应用
CN100352928C (zh) * 2005-06-28 2007-12-05 南京工业大学 一种生产l－苯丙氨酸基因工程菌及构建方法和其应用
ES2800341T3 (es) 2012-07-11 2020-12-29 Adisseo France Sas Método para la preparación de 2,4-dihidroxibutirato
ES1254139Y (es) 2020-07-08 2021-01-12 Ocean Kube Env S L Contenedor para la recogida de residuos urbanos
CN111926024B (zh) * 2020-08-18 2022-07-08 南京农业大学 OsDNR1基因的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8301700D0 (en) * 1983-01-21 1983-02-23 Searle & Co Cloning and utilisation of aminotransferase genes
US4600692A (en) * 1983-02-10 1986-07-15 Purification Engineering, Inc. Immobilized cells for preparing phenylalanine
GB8403244D0 (en) * 1984-02-07 1984-03-14 Searle & Co Aminoacids via bioconversion
US4783403A (en) * 1984-02-08 1988-11-08 Kyowa Hakko Kogyo Co., Ltd. Process for producing l-phenylalanine
DE3713755A1 (de) * 1987-04-24 1988-11-10 Hoechst Ag Herstellung von l-phenylalanin mit hilfe rekombinanter bakterien

Also Published As

Publication number Publication date
ATE92104T1 (de) 1993-08-15
JPH09107966A (ja) 1997-04-28
IE872530L (en) 1988-03-19
US5091314A (en) 1992-02-25
EP0260597A3 (en) 1989-07-19
PT85752A (de) 1987-10-01
IL83934A0 (en) 1988-02-29
NO873919D0 (no) 1987-09-18
DE3786728D1 (de) 1993-09-02
DE3631829A1 (de) 1988-07-28
JP2760973B2 (ja) 1998-06-04
AU7866887A (en) 1988-03-24
JP2760788B2 (ja) 1998-06-04
PT85752B (pt) 1990-08-31
FI874058A (fi) 1988-03-20
CA1341096C (en) 2000-09-12
EP0260597B1 (de) 1993-07-28
EP0260597A2 (de) 1988-03-23
ZA877031B (en) 1988-04-27
NO873919L (no) 1988-03-21
DK490187A (da) 1988-03-20
JPS6398390A (ja) 1988-04-28
FI874058A0 (fi) 1987-09-17
DK490187D0 (da) 1987-09-18
CN87106923A (zh) 1988-06-22

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