KR880000591A - 인체 리소자임의 제조방법 - Google Patents

인체 리소자임의 제조방법 Download PDF

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Publication number
KR880000591A
KR880000591A KR1019870006654A KR870006654A KR880000591A KR 880000591 A KR880000591 A KR 880000591A KR 1019870006654 A KR1019870006654 A KR 1019870006654A KR 870006654 A KR870006654 A KR 870006654A KR 880000591 A KR880000591 A KR 880000591A
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KR
South Korea
Prior art keywords
human lysozyme
dna sequence
dna
portion encoding
pgfl735
Prior art date
Application number
KR1019870006654A
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English (en)
Inventor
마사가즈 기구찌
고오지 요시무라
가즈오 나까하마
Original Assignee
우메모또 요시마사
다께다 야꾸힝 고오교 가부시끼가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 우메모또 요시마사, 다께다 야꾸힝 고오교 가부시끼가이샤 filed Critical 우메모또 요시마사
Publication of KR880000591A publication Critical patent/KR880000591A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/94—Saccharomyces
    • Y10S435/942—Saccharomyces cerevisiae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

인체 리소자임의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 올리고뉴클레오티드를 수거 및 결찰시켜 인체리소자임을 합성하는 방법을 나타내는 도표이고
제2도는 인체리소자임 유전자의 Taq I -Xho I 단편의 DNA 서열을 나타내며
제4도는 인체리소자임 분비플라스미드의 구조도식을 나타낸다.

Claims (6)

  1. 하기식으로 나타내어지는 시그날 펩티드를 코오딩하는 부분이 인체리소자임을 코오딩하는 DNA 부분의 5'-말단과 결합되어 있는 DNA 서열.
    M-R-S-F-L-L-L-A-L-C-F-L-P-L-A-A-L-G
  2. 제1항에 있어서, 시그날 펩티드를 코오딩하는 DNA 부분이 제3도에 나타낸 DNA 서열을 갖는 DNA 서열.
  3. 제1항에 있어서, pGFL735인 DNA 서열.
  4. 제1, 3 또는 3항에서 청구한 DNA 부분으로 형질전환시킨 세포.
  5. 제4항에 있어서, 사카로미세스 세레비시아에 AH22R-/pGFL735(FERM BP-1346)인 세포.
  6. 제4항에서 청구한 세포를 배양하고, 배양물중에 인체리소자임을 축적시키고, 이어서 그를 회수함을 특징으로하는 인체리소자임의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870006654A 1986-06-30 1987-06-29 인체 리소자임의 제조방법 KR880000591A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP151809 1983-08-19
JP15180986 1986-06-30
JP19263986 1986-08-20
JP192639 1986-08-20
JP37869 1987-02-23
JP3786987 1987-02-23

Publications (1)

Publication Number Publication Date
KR880000591A true KR880000591A (ko) 1988-03-28

Family

ID=27289624

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1019870006653A KR880000587A (ko) 1986-06-30 1987-06-29 리소자임의 제조방법
KR1019870006654A KR880000591A (ko) 1986-06-30 1987-06-29 인체 리소자임의 제조방법

Family Applications Before (1)

Application Number Title Priority Date Filing Date
KR1019870006653A KR880000587A (ko) 1986-06-30 1987-06-29 리소자임의 제조방법

Country Status (6)

Country Link
US (1) US4945051A (ko)
EP (2) EP0251730A3 (ko)
KR (2) KR880000587A (ko)
CN (2) CN87104541A (ko)
CA (1) CA1305439C (ko)
TW (1) TW205070B (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3540075A1 (de) * 1985-11-12 1987-05-14 Boehringer Ingelheim Int Human-lysozym
US5182195A (en) * 1987-11-13 1993-01-26 Takeda Chemical Industries, Ltd. Method for increasing gene expression using protease deficient yeasts
CN1064082C (zh) * 1994-06-14 2001-04-04 北京市联合大学 生物酶素揭裱液及其揭裱方法
FR2768748B1 (fr) * 1997-09-24 2001-06-08 Rhone Poulenc Agrochimie Recodage de sequences d'adn permettant leur expression dans les levures et levures transformees obtenues
US6787337B1 (en) 1997-09-24 2004-09-07 Aventis Cropscience S.A. Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467577A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex pcr
ITMI20061950A1 (it) * 2006-10-11 2008-04-12 Therapicon Srl Effetto antimetastatico in condizioni patologiche delle cellule umane
WO2008109401A2 (en) * 2007-03-02 2008-09-12 Saint Simeon Lda Composition and method for treating papilloma virus and equine sarcoids
WO2008109397A2 (en) * 2007-03-02 2008-09-12 Saint Simeon Lda Novel ophthalmic compositions containing human recombinant lysozyme and use thereof for treating eye conditions and as contact lens solutions
CN109799335B (zh) * 2019-01-31 2022-04-01 陕西慧康生物科技有限责任公司 重组人溶菌酶中毕赤酵母宿主蛋白残留量的检测方法
CN115386561B (zh) * 2022-05-09 2024-07-16 上海大学 一种人源溶菌酶蛋白基因、其表达载体的构建方法及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4637980A (en) * 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0155189A3 (en) * 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
EP0170266B1 (en) * 1984-07-30 1993-03-24 Wakunaga Seiyaku Kabushiki Kaisha Process for producing protein, and vector, recombinant dna and transformant used therefor
JPS61158793A (ja) * 1984-11-14 1986-07-18 Takeda Chem Ind Ltd ヒト・リゾチ−ムの合成遺伝子
BE901223A (fr) * 1984-12-06 1985-03-29 Labofina Sa Levures transformees produisant du lysozyme plasmides utilises pour cette transformation et leurs utilisations.
JPS626679A (ja) * 1985-06-26 1987-01-13 Agency Of Ind Science & Technol ヒトリゾチ−ムの微生物学的製造法

Also Published As

Publication number Publication date
CN87104540A (zh) 1988-02-24
US4945051A (en) 1990-07-31
TW205070B (ko) 1993-05-01
EP0255233A2 (en) 1988-02-03
KR880000587A (ko) 1988-03-28
EP0251730A2 (en) 1988-01-07
EP0255233A3 (en) 1988-11-02
CA1305439C (en) 1992-07-21
EP0251730A3 (en) 1988-11-02
CN87104541A (zh) 1988-02-10

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