KR870006902A - 백일해 항원의 정제방법 - Google Patents
백일해 항원의 정제방법 Download PDFInfo
- Publication number
- KR870006902A KR870006902A KR870000366A KR870000366A KR870006902A KR 870006902 A KR870006902 A KR 870006902A KR 870000366 A KR870000366 A KR 870000366A KR 870000366 A KR870000366 A KR 870000366A KR 870006902 A KR870006902 A KR 870006902A
- Authority
- KR
- South Korea
- Prior art keywords
- ciliated
- lpf
- separating
- fha
- aggregate
- Prior art date
Links
- 201000005702 Pertussis Diseases 0.000 title 1
- 239000000427 antigen Substances 0.000 title 1
- 102000036639 antigens Human genes 0.000 title 1
- 108091007433 antigens Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 229920002684 Sepharose Polymers 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 8
- 101710154643 Filamentous hemagglutinin Proteins 0.000 claims 7
- 239000006228 supernatant Substances 0.000 claims 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims 2
- 239000003102 growth factor Substances 0.000 claims 2
- 230000000527 lymphocytic effect Effects 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 241000588832 Bordetella pertussis Species 0.000 claims 1
- 102000013361 fetuin Human genes 0.000 claims 1
- 108060002885 fetuin Proteins 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 238000009630 liquid culture Methods 0.000 claims 1
- 229910001629 magnesium chloride Inorganic materials 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000010828 elution Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K2039/10—Brucella; Bordetella, e.g. Bordetella pertussis; Not used, see subgroups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Communicable Diseases (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 pH 6.5의 0.1M인산-0.5M NaCl 완충액에 의한 하이드록실인회석 크로마토그래피로부터 FHA의 용리기록.
제2도 pH 7.2의 0.05M인산-0.5M NaCl 완충액에 의한 세파로스 CL-6B 크로마토그래피로부터 FHA의 용리기록.
Claims (9)
- (a) 세포내의 배양균 및 상등액 분설을 분리하고, (b) 상등액 분설을 농축시키고, (c) 농축된 상등액 분설을 분별하여 LPF 및 FHA를 함유한 분설을 분리시키고, (d) 세포의 분설로부터 최소한 하나의 섬모형 응집원을 분리시키는 것으로 구성되는 Bordetella pertussis의 액체 배양균으로부터 임파구증가 조장 인자(LPF), 사상체의 혈구응집소(FHA) 및 최소한 하나의 섬모형 응집원을 제조하는 방법.
- 제1항에 있어서, 단계(d)에서 분리된 섬모형 응집원이 최소한 하나의 응집원 2,3,4,5 및 6을 포함하는 것인 방법.
- 제1항에 있어서, 단계(d)에서 분리된 섬모형 응집원이 최소한 응집원 2 및 3(Ag2+3)으로 구성되는 것인 방법.
- 제3항에 있어서, 분리된 응집원이 부가적으로 하나 이상의 응집원 4,5 및 6을 포함하는 것인 방법.
- 전술한 항의 어느 한항에 있어서, 분리단계(a)를 pH 7.0이상에서 행하는 방법.
- 제5항에 있어서, 분리단계(a)를 pH 7.5-9.0범위에서 행하는 방법.
- 전술한 항의 어느 한항에 있어서, 단계(b)에서 상등액을 처음 부피의 50%이하로 및 바람직하게는 25%이하로 농축시키는 방법.
- 전술한 항의 어느 한항에 있어서, 단계(c)에서 LPF를 함유한 분설을 페튄세파로스(fetuin sepharose)에 흡착시킨 다음 염화마그네슘 완충액으로 용리하여 정제하는 방법.
- 제1항 내지 제8항의 어느 한항에 따라 제조한 (i) LPF, (ii) FHA 및 (iii) 최소한 하나의 섬모형 응집원이 혼합되어 있고, LPF, FHA 및 최소한 하나의 섬모형 응집원이 혼합하기전 또는 후에 해독된, 임파구증가 조장인자(LPF), 사상체의 혈구응집소(FHA) 및 최소한 하나의 섬모형 응집원으로 구성된 왁진 조성물의 제조방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8601279 | 1986-01-20 | ||
GB868601279A GB8601279D0 (en) | 1986-01-20 | 1986-01-20 | Purification of pertussis antigens |
Publications (2)
Publication Number | Publication Date |
---|---|
KR870006902A true KR870006902A (ko) | 1987-08-13 |
KR950010323B1 KR950010323B1 (ko) | 1995-09-14 |
Family
ID=10591636
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019870000366A KR950010323B1 (ko) | 1986-01-20 | 1987-01-19 | 백일해 항원의 정제 방법 |
Country Status (11)
Country | Link |
---|---|
US (1) | US4784589A (ko) |
EP (1) | EP0231083B1 (ko) |
JP (1) | JP2538224B2 (ko) |
KR (1) | KR950010323B1 (ko) |
CN (1) | CN1028206C (ko) |
AT (1) | ATE92335T1 (ko) |
AU (1) | AU592727B2 (ko) |
CA (1) | CA1280693C (ko) |
DE (1) | DE3786806T2 (ko) |
DK (1) | DK172936B1 (ko) |
GB (1) | GB8601279D0 (ko) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1337859C (en) * | 1987-04-24 | 1996-01-02 | Masashi Chazono | Method for culturing bordetella pertussis, a pertussis toxoid and a pertussis vaccine |
GB8807860D0 (en) * | 1988-04-05 | 1988-05-05 | Connaught Lab | Pertussis vaccine |
CA1341123C (en) * | 1988-10-27 | 2000-10-17 | David A. Relman | Filamentous hemagglutinin of b. pertussis |
JP2706792B2 (ja) * | 1988-11-29 | 1998-01-28 | 財団法人化学及血清療法研究所 | 百日咳毒素のトキソイド化法 |
GB8910570D0 (en) * | 1989-05-08 | 1989-06-21 | Wellcome Found | Acellular vaccine |
US5391715A (en) * | 1989-11-06 | 1995-02-21 | Smithkline Beecham Biologicals | Method for isolating and purifying bordetella pertussis antigenic factors |
DE69025375T2 (de) | 1989-11-06 | 1996-08-01 | Smithkline Beecham Biolog | Verfahren |
JP3035712B2 (ja) * | 1990-02-12 | 2000-04-24 | スミスクライン ビーチャム バイオロジカルズ(エス ア) | 新規なワクチンおよびそのための方法 |
EP0484621A3 (en) * | 1990-07-11 | 1992-08-26 | American Cyanamid Company | Efficacious vaccines against bordetella pertussis comprising a combination of individually purified pertussis antigens |
US5445817A (en) * | 1992-08-21 | 1995-08-29 | The United States Of America As Represented By The Department Of Health And Human Services | Pertussis toxin used as a carrier protein with non-charged saccharides in conjugate vaccines |
WO1995029934A1 (en) * | 1994-04-28 | 1995-11-09 | Takeda Chemical Industries, Ltd. | Method of separating protective components of bordetella pertussis |
EP1234579A1 (en) * | 1995-05-04 | 2002-08-28 | Aventis Pasteur Limited | Acellular Pertussis Vaccines and Methods of Preparation Thereof |
US7091311B2 (en) * | 1996-06-07 | 2006-08-15 | Smithkline Beecham Corporation | Peptides and compounds that bind to a receptor |
MXPA05003057A (es) | 2002-09-18 | 2006-04-18 | Johnson & Johnson | Metodos para aumentar la produccion de celulas madre hematopoyeticas y plaquetas. |
MY172181A (en) | 2013-03-08 | 2019-11-15 | Janssen Vaccines & Prevention Bv | Acellular pertussis vaccine |
CN110358802B (zh) * | 2019-08-16 | 2021-06-18 | 长春百克生物科技股份公司 | 一种去除百日咳组分菌毛蛋白2/3内毒素的方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4247452A (en) * | 1978-03-01 | 1981-01-27 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Purification of pertussis haemagglutinins |
JPS5750925A (en) * | 1980-09-12 | 1982-03-25 | Takeda Chem Ind Ltd | Preparation of pertussis toxoid |
US4474758A (en) * | 1981-11-19 | 1984-10-02 | American Cyanamid Company | Haemophilus influenzae type b and pertussis outer membrane component combined vaccine |
HU188847B (en) * | 1983-02-22 | 1986-05-28 | Human Oltoanyagtermeloe Es Kutato Intezet,Hu | Process for producing liophylized combined vaccines |
CA1213234A (en) * | 1983-03-30 | 1986-10-28 | Akihiro Ginnaga | Method for the production of ha fraction containing protective antigens of bordetella pertussis and pertussis vaccine |
US4551429A (en) * | 1983-09-15 | 1985-11-05 | American Home Products Corporation | Stimulation of antigen production by Bordetella pertussis |
JPS6098988A (ja) * | 1983-11-01 | 1985-06-01 | Chemo Sero Therapeut Res Inst | Lpf−haの精製法 |
AU571078B2 (en) * | 1984-04-14 | 1988-03-31 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Purification of filamentous hemagglutinin |
-
1986
- 1986-01-20 GB GB868601279A patent/GB8601279D0/en active Pending
-
1987
- 1987-01-16 DE DE87300387T patent/DE3786806T2/de not_active Expired - Lifetime
- 1987-01-16 EP EP87300387A patent/EP0231083B1/en not_active Expired - Lifetime
- 1987-01-16 AT AT87300387T patent/ATE92335T1/de not_active IP Right Cessation
- 1987-01-19 KR KR1019870000366A patent/KR950010323B1/ko not_active IP Right Cessation
- 1987-01-19 CA CA000527592A patent/CA1280693C/en not_active Expired - Lifetime
- 1987-01-19 AU AU67664/87A patent/AU592727B2/en not_active Expired
- 1987-01-19 DK DK198700272A patent/DK172936B1/da not_active IP Right Cessation
- 1987-01-20 CN CN87100399A patent/CN1028206C/zh not_active Expired - Lifetime
- 1987-01-20 JP JP62009178A patent/JP2538224B2/ja not_active Expired - Lifetime
- 1987-01-20 US US07/008,880 patent/US4784589A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK27287D0 (da) | 1987-01-19 |
DK27287A (da) | 1987-07-21 |
ATE92335T1 (de) | 1993-08-15 |
US4784589A (en) | 1988-11-15 |
DK172936B1 (da) | 1999-10-11 |
EP0231083A3 (en) | 1988-06-08 |
EP0231083A2 (en) | 1987-08-05 |
JPS62234031A (ja) | 1987-10-14 |
CN1028206C (zh) | 1995-04-19 |
DE3786806D1 (de) | 1993-09-09 |
AU6766487A (en) | 1987-07-23 |
CA1280693C (en) | 1991-02-26 |
GB8601279D0 (en) | 1986-02-26 |
DE3786806T2 (de) | 1993-12-09 |
AU592727B2 (en) | 1990-01-18 |
CN87100399A (zh) | 1987-09-09 |
JP2538224B2 (ja) | 1996-09-25 |
EP0231083B1 (en) | 1993-08-04 |
KR950010323B1 (ko) | 1995-09-14 |
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