KR870005649A - 고상화항체 및 그 제조방법 - Google Patents

고상화항체 및 그 제조방법 Download PDF

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KR870005649A
KR870005649A KR860010368A KR860010368A KR870005649A KR 870005649 A KR870005649 A KR 870005649A KR 860010368 A KR860010368 A KR 860010368A KR 860010368 A KR860010368 A KR 860010368A KR 870005649 A KR870005649 A KR 870005649A
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peptide
immunoglobulin
antibody
solidifying
solid carrier
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KR860010368A
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KR910004141B1 (ko
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데쓰오 쓰지
마사오 고노
이노우에겐
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요시도시 가즈오
시오노기 세이야꾸 가부시끼가이샤
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/62—Insulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음

Description

고상화항체 밑 그 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (13)

  1. 수불용성담체상에 헤테로이관능성 가교제에 의하여서 고상화 시키는데 있어서, 항체자 인푸로인슐린 C-펩타이드에 특이적인 부분 환원혀의 임뮤노글로불린으로 치룰기를 갖는 고상화 항체.
  2. 제1항에 있어서, 수불용성 고형담체가 아미노기를 갖는 것이 특징인 고상화 항체.
  3. 제1항에 있어서, 헤테로이관능성 가교체가 두가지 결합을 형성할 수 있는데, 하나는 임뮤노글로불린의 이촐기와 타하나는 고형 담체의 아미노 그룹과 결합하는 것이 특징인 고상화 항체.
  4. 제1항에 있어서, 고형담체가 아미노기를 갖는 미세과립 폴리아크릴아미드 겔이 특징인 고상화 항체.
  5. 제4항에 있어서, 미세과립 폴리아크리아미드 겔이 부분적으로 가수분해되고 직쇄형 알킬렌디아민과 커플링 반응하여 푸리아미노기를 갖게 하는 것이 특징인 고상화항체.
  6. 제1항에 있어서, 헤테로이관능성 가교제가 m-말레이미도벤조인산N--히드록시호박산이미드 에스테르(MBS)가 특징인 고상화항체.
  7. 임뮤노글로불린과 고형담체와의 사이에 고형담체가 갖고 있는 아미노기와 임뮤노글로불린이 갖고 있는 치올기가 가교제의 아미노기 지향성 관능기와 치올기 지향성 관능기와 각각 가교를 형성하는 것에의하여서, 헤테로이 관능성가교제의 보조하에 수불용성 담체와 인푸로인슐린 C-펩타이드레 특이적인 임뮤노글로불린의 부분환원형의 가교결합을 특징으로 하는 고상화항체의 제조방법.
  8. 제7항에 있어서, 수불용성 고형담체가 아미노기를 함유하는 미세과립 플로아크릴아미드겔인 고상화 항체의 제조방법.
  9. 제7항에 있어서, 수불용성 고형담체가 직쇄형 알킬렌디아민과 커플링반응함으로서 도입된 푸리아미노기를 함유하는 부분적으로 가수분해된 미세과립 폴리아크릴아미드겔인 고상화 항체에 제조방법.
  10. 제7항에 있어서, 헤테로이관능성가교제가 m-말레이미드벤조인산 N-히드록시호박산 에스테르 (MBS)인 고상화항체의 제조방법.
  11. 제7항에 있어서, 부분환원형 임뮤노글로부린이 인푸로인슐린 C-펩타이드 또는 그 푸라그멘트를 리간드로 갖고 있는 면역치화 칼람상에서 정제한 후 부분환원을 행한 인푸로인슐린 C-펩타이드에 특이적인 혈청에서 부터 수득한 임뮤노글로불린인 고상화항체의 제조방법.
  12. 헤테로이관능성가교제의 보조하에 아미노기를 갖는 수불용성 고형담체와 인푸로인슐린 C-펩타이드에 특이적인 부분환원형 임뮤노글로부린과 결합함으로써 얻어지는 고상화 항체를 이용하는 것을 특징으로 하는 단항체법에 근거한 인푸로인슐린 C-펩타이드의 면역측정방법.
  13. 헤테로이관능성가교제, 표준 인푸로인슐린 C-펩타이드용액 및 방사능이 있는 표지를 한 인푸로인슐린 C-펩타이드 유도체와 완충제의 보조하에 아미노기를 갖는 수불용성 고형담체와 인푸로인슐린 C-펩타이드에 특이적인 부분환원형의 임뮤노글로불린을 결합시켜서 얻어지는 고상화 항체를 특징으로 하여, 체액중의 인푸로인슐린 C-펩타이드를 측정하는데 사용하는 방사능 면역측정 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860010368A 1985-12-04 1986-12-04 고상화 c 펩타이드 항체, 그 제조방법 및 동 물질을 이용하는 면역 측정법 KR910004141B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP85-273825 1985-12-04
JP60273825A JPS62132172A (ja) 1985-12-04 1985-12-04 固相化抗体およびその製造方法
JP60-273825 1985-12-04

Publications (2)

Publication Number Publication Date
KR870005649A true KR870005649A (ko) 1987-07-06
KR910004141B1 KR910004141B1 (ko) 1991-06-22

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EP (1) EP0227351B1 (ko)
JP (1) JPS62132172A (ko)
KR (1) KR910004141B1 (ko)
CA (1) CA1283852C (ko)
DE (1) DE3682491D1 (ko)
GB (1) GB2184127B (ko)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3640412A1 (de) * 1986-11-26 1988-06-09 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
JPS6488367A (en) * 1987-09-30 1989-04-03 Japan Chem Res Immobilized antibody
ES2099699T3 (es) * 1989-05-02 1997-06-01 Abbott Lab Fijacion covalente de los miembros de union especificos a las fases solidas.
US5279954A (en) * 1989-06-30 1994-01-18 Board Of Regents Of The University Of Nebraska And Bionebraska Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
JPH0346130A (ja) * 1989-07-14 1991-02-27 Matsushita Electric Ind Co Ltd 光記録再生装置
WO1991004753A1 (en) * 1989-10-02 1991-04-18 Cetus Corporation Conjugates of antisense oligonucleotides and therapeutic uses thereof
GB8927365D0 (en) * 1989-12-04 1990-01-31 Ici Plc Reagent
JP3044569B2 (ja) 1990-11-09 2000-05-22 東ソー株式会社 ヒトｃ―ペプチドの測定方法
US5436147A (en) * 1991-03-01 1995-07-25 Nucleic Assays Corporation Heterobifunctional crosslinked agents for immobilizing molecules on plastic substrates
US5279955A (en) * 1991-03-01 1994-01-18 Pegg Randall K Heterofunctional crosslinking agent for immobilizing reagents on plastic substrates
WO1993000586A1 (en) * 1991-06-26 1993-01-07 Obschestvo S Ogranichennoi Otvetstvennostju 'dies' Immunosorbent for diagnosis of epilepsy and group of risk
CN113049811A (zh) * 2021-03-22 2021-06-29 深圳市亚辉龙生物科技股份有限公司 纳米磁珠包被物及其制备方法、检测试剂和检测试剂盒
CN114130377A (zh) * 2021-12-14 2022-03-04 无锡创谱生物科技有限公司 一种亲和层析填料、其制备方法及用途

Family Cites Families (8)

* Cited by examiner, † Cited by third party
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GB2013688B (en) * 1978-01-26 1982-06-30 Technicon Instr Insolubilised proteins and immunoassays utilising them
JPS55105653A (en) * 1978-10-02 1980-08-13 Shionogi & Co Ltd Tyrosyl c-peptides
JPS56168159A (en) * 1980-05-29 1981-12-24 Sekisui Chem Co Ltd Method for measurement of antigen or antibody
JPS5745454A (en) * 1980-09-02 1982-03-15 Fuji Photo Film Co Ltd Immunochemical measuring method for various minor components
US4429008B1 (en) * 1981-12-10 1995-05-16 Univ California Thiol reactive liposomes
JPS59176675A (ja) * 1983-03-26 1984-10-06 Eiken Kagaku Kk 酵素免疫測定用試薬
EP0134307B1 (de) * 1983-07-29 1989-04-19 Henning Berlin GmbH Chemie und Pharmawerk Voraktivierte Kunststoffoberflächen zur Immobilisierung von organisch-chemischen und biologischen Materialien, Verfahren zur Herstellung und Verwendung derselben
JPH0753759B2 (ja) * 1984-10-29 1995-06-07 オリエンタル酵母工業株式会社 免疫吸着体及びその製造法

Also Published As

Publication number Publication date
GB2184127B (en) 1990-04-25
EP0227351B1 (en) 1991-11-13
EP0227351A2 (en) 1987-07-01
CA1283852C (en) 1991-05-07
EP0227351A3 (en) 1988-09-07
KR910004141B1 (ko) 1991-06-22
GB2184127A (en) 1987-06-17
JPS62132172A (ja) 1987-06-15
DE3682491D1 (de) 1991-12-19
GB8628882D0 (en) 1987-01-07

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