JPS6421367A - Solid phase antibody for immunoassay - Google Patents
Solid phase antibody for immunoassayInfo
- Publication number
- JPS6421367A JPS6421367A JP17597687A JP17597687A JPS6421367A JP S6421367 A JPS6421367 A JP S6421367A JP 17597687 A JP17597687 A JP 17597687A JP 17597687 A JP17597687 A JP 17597687A JP S6421367 A JPS6421367 A JP S6421367A
- Authority
- JP
- Japan
- Prior art keywords
- immunoassay
- solid phase
- antibody
- phase antibody
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE:To enhance the detection sensitivity in immunoassay by increasing the adsorbing amount of an antibody, by using a zirconia ceramic ball as a solid phase. CONSTITUTION:A zirconia ceramic ball having a diameter of 6.5mmphi is immersed in a 0.01mol./l (pH 7.5) phosphate buffer solution containing 0.1mg/ml of anti-AFP-IgG at 4 deg.C overnight and subsequently washed three times and further washed three times with a liquid mixture of a 0.01mol./l (pH 7.0) phosphate buffer solution, 0.1mol./l of NaCl, 0.1% of BSA and 0.1% of NaN3. By this method, the adsorbing amount of an antibody by a solid phase can be increased and the detection sensitivity in immunoassay can be enhanced.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17597687A JPS6421367A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17597687A JPS6421367A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6421367A true JPS6421367A (en) | 1989-01-24 |
Family
ID=16005532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17597687A Pending JPS6421367A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6421367A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5780408A (en) * | 1993-06-30 | 1998-07-14 | Mmz Solutions Pty. Ltd. | Body moulding solution |
-
1987
- 1987-07-16 JP JP17597687A patent/JPS6421367A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5780408A (en) * | 1993-06-30 | 1998-07-14 | Mmz Solutions Pty. Ltd. | Body moulding solution |
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