JPS6421365A - Solid phase antibody for immunoassay - Google Patents
Solid phase antibody for immunoassayInfo
- Publication number
- JPS6421365A JPS6421365A JP17597787A JP17597787A JPS6421365A JP S6421365 A JPS6421365 A JP S6421365A JP 17597787 A JP17597787 A JP 17597787A JP 17597787 A JP17597787 A JP 17597787A JP S6421365 A JPS6421365 A JP S6421365A
- Authority
- JP
- Japan
- Prior art keywords
- immunoassay
- solid phase
- antibody
- phase antibody
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To enhance the detection sensitivity in immunoassay by increasing the adsorbing amount of an antibody, by using a carbon ball as a solid phase. CONSTITUTION:A carbon ball having a diameter of 6.5mmphi is immersed in a 0.1mol./l (pH 7.5) phosphate buffer solution containing 0.1mg/ml of anti- AFT-IgG at 4 deg.C overnight and subsequently washed three times and further washed three times with a liquid mixture of a 0.01mol./l (pH 7.0) phosphate buffer solution, 0.1mol./l of NaCl, 0.1% BSA and 0.1% NaN3. By this method, the adsorbing amount of an antibody by a solid phase can be increased and the detection sensitivity in immunoassay can be enhanced.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17597787A JPS6421365A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17597787A JPS6421365A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6421365A true JPS6421365A (en) | 1989-01-24 |
Family
ID=16005548
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17597787A Pending JPS6421365A (en) | 1987-07-16 | 1987-07-16 | Solid phase antibody for immunoassay |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6421365A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6278086U (en) * | 1985-11-01 | 1987-05-19 | ||
GB2239314B (en) * | 1989-12-18 | 1994-05-18 | Princeton Biomeditech Corp | Carbon black having an immunologically-active compound bound thereto |
-
1987
- 1987-07-16 JP JP17597787A patent/JPS6421365A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6278086U (en) * | 1985-11-01 | 1987-05-19 | ||
GB2239314B (en) * | 1989-12-18 | 1994-05-18 | Princeton Biomeditech Corp | Carbon black having an immunologically-active compound bound thereto |
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