GB2011070A - Assay of vitamin B12 - Google Patents

Assay of vitamin B12

Info

Publication number
GB2011070A
GB2011070A GB7847509A GB7847509A GB2011070A GB 2011070 A GB2011070 A GB 2011070A GB 7847509 A GB7847509 A GB 7847509A GB 7847509 A GB7847509 A GB 7847509A GB 2011070 A GB2011070 A GB 2011070A
Authority
GB
United Kingdom
Prior art keywords
vitamin
assay
solution
chicken serum
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7847509A
Other versions
GB2011070B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Technicon Instruments Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technicon Instruments Corp filed Critical Technicon Instruments Corp
Priority to GB7847509A priority Critical patent/GB2011070B/en
Publication of GB2011070A publication Critical patent/GB2011070A/en
Application granted granted Critical
Publication of GB2011070B publication Critical patent/GB2011070B/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The amount of vitamin B12 in solution in a liquid is determined by mixing a sample of the solution with vitamin B12-binding proteins from chicken serum in a pH of about 12.8 to 13.2, and then analysing a component of the mixture. In preferred procedures, especially useful in assaying human sera, a competitive binding technique is employed utilizing labelled vitamin B12 and a buffer containing cyanide ion. By operating at very high pH and with chicken serum proteins, the assay can be conducted relatively simply and with very accurate results.
GB7847509A 1977-12-14 1978-12-07 Assay of vitamin b12 Expired GB2011070B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB7847509A GB2011070B (en) 1977-12-14 1978-12-07 Assay of vitamin b12

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB5206977 1977-12-14
GB7847509A GB2011070B (en) 1977-12-14 1978-12-07 Assay of vitamin b12

Publications (2)

Publication Number Publication Date
GB2011070A true GB2011070A (en) 1979-07-04
GB2011070B GB2011070B (en) 1982-07-14

Family

ID=26266987

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7847509A Expired GB2011070B (en) 1977-12-14 1978-12-07 Assay of vitamin b12

Country Status (1)

Country Link
GB (1) GB2011070B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3137668A1 (en) 1980-09-22 1982-05-27 Amersham International Ltd., Amersham, Buckinghamshire METHOD FOR TESTING FOR VITAMIN B (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) 2 (DOWN ARROW) AND TEST SET FOR ITS IMPLEMENTATION
US4332786A (en) * 1980-05-15 1982-06-01 Baxter Travenol Laboratories, Inc. Method and composition for vitamin B-12 Assay

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4332786A (en) * 1980-05-15 1982-06-01 Baxter Travenol Laboratories, Inc. Method and composition for vitamin B-12 Assay
DE3137668A1 (en) 1980-09-22 1982-05-27 Amersham International Ltd., Amersham, Buckinghamshire METHOD FOR TESTING FOR VITAMIN B (DOWN ARROW) 1 (DOWN ARROW) (DOWN ARROW) 2 (DOWN ARROW) AND TEST SET FOR ITS IMPLEMENTATION

Also Published As

Publication number Publication date
GB2011070B (en) 1982-07-14

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Legal Events

Date Code Title Description
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19941207