KR20250099140A - Fad형 글루타민산 디히드로게나제 - Google Patents

Fad형 글루타민산 디히드로게나제 Download PDF

Info

Publication number
KR20250099140A
KR20250099140A KR1020257014167A KR20257014167A KR20250099140A KR 20250099140 A KR20250099140 A KR 20250099140A KR 1020257014167 A KR1020257014167 A KR 1020257014167A KR 20257014167 A KR20257014167 A KR 20257014167A KR 20250099140 A KR20250099140 A KR 20250099140A
Authority
KR
South Korea
Prior art keywords
amino acid
sequence number
fad
sequence
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020257014167A
Other languages
English (en)
Korean (ko)
Inventor
케이타로 쿠제
케이타 토다
아츠시 이치야나기
Original Assignee
기꼬만 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 기꼬만 가부시키가이샤 filed Critical 기꼬만 가부시키가이샤
Publication of KR20250099140A publication Critical patent/KR20250099140A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020257014167A 2022-10-27 2023-10-27 Fad형 글루타민산 디히드로게나제 Pending KR20250099140A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2022-172293 2022-10-27
JP2022172293 2022-10-27
PCT/JP2023/038908 WO2024090562A1 (ja) 2022-10-27 2023-10-27 Ｆａｄ型グルタミン酸デヒドロゲナーゼ

Publications (1)

Publication Number Publication Date
KR20250099140A true KR20250099140A (ko) 2025-07-01

Family

ID=90831045

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020257014167A Pending KR20250099140A (ko) 2022-10-27 2023-10-27 Fad형 글루타민산 디히드로게나제

Country Status (4)

Country Link
EP (1) EP4610358A1 (https=)
JP (1) JPWO2024090562A1 (https=)
KR (1) KR20250099140A (https=)
WO (1) WO2024090562A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2023140286A1 (https=) * 2022-01-18 2023-07-27

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019097420A (ja) 2017-11-29 2019-06-24 ヤマサ醤油株式会社 Ｌ−グルタミン酸オキシダーゼ乾燥組成物
JP2021193598A (ja) 2017-06-29 2021-12-23 アマゾン テクノロジーズ インコーポレイテッド セキュリティポリシーアナライザサービス及び充足可能性エンジン

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02163080A (ja) * 1989-04-11 1990-06-22 Toyo Jozo Co Ltd Ｌ―グルタミン酸オキシダーゼ（ｈ↓２ｏ↓２・ジエネレイテイング）およびその製造法
JP2007222055A (ja) 2006-02-22 2007-09-06 Noda Inst For Scient Res 染色体領域欠失株の作製方法
JP2011239681A (ja) 2008-09-19 2011-12-01 Kikkoman Corp 麹菌アルカリプロテアーゼプロモーター
JP2014153243A (ja) * 2013-02-12 2014-08-25 Tanita Corp バイオセンサおよびこれを用いた測定方法
KR102213905B1 (ko) 2014-08-06 2021-02-10 인터디지탈 패튼 홀딩스, 인크 디바이스-대-디바이스(d2d) 선점 및 액세스 제어
CN109266664B (zh) * 2018-10-23 2022-02-08 南京工业大学 一种利用融合截短表达策略提高谷氨酸氧化酶稳定性的方法
EP4130023A4 (en) 2020-03-24 2024-04-24 Ajinomoto Co., Inc. L-GLUTAMIC ACID OXIDAZE MUTANT
JPWO2023140286A1 (https=) * 2022-01-18 2023-07-27

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021193598A (ja) 2017-06-29 2021-12-23 アマゾン テクノロジーズ インコーポレイテッド セキュリティポリシーアナライザサービス及び充足可能性エンジン
JP2019097420A (ja) 2017-11-29 2019-06-24 ヤマサ醤油株式会社 Ｌ−グルタミン酸オキシダーゼ乾燥組成物

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
비특허문헌 1: Kusakabe H et al., Agric. Biol. Chem., 47, 1323-1328(1983)
비특허문헌 2: Arima J et al., J. Biochem., 134, 805-812(2003)

Also Published As

Publication number Publication date
WO2024090562A1 (ja) 2024-05-02
EP4610358A1 (en) 2025-09-03
JPWO2024090562A1 (https=) 2024-05-02

Similar Documents

Publication Publication Date Title
US8828699B2 (en) Eukaryotic amadoriase, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of the eukaryotic amadoriase
CN102292439B (zh) 黄素结合型葡萄糖脱氢酶
JP6176922B2 (ja) 基質特異性が改変されたアマドリアーゼ
EP2003199B1 (en) Glucose dehydrogenase
JP2019103500A (ja) フラビン結合型グルコースデヒドロゲナーゼ、フラビン結合型グルコースデヒドロゲナーゼの製造方法、およびそれを用いたグルコース測定方法
CA2891285C (en) Novel glucose oxidases derived from aspergillus niger
KR20130038914A (ko) 글루코스 탈수소효소
US9506042B2 (en) Glucose dehydrogenase
WO2017094776A1 (ja) シトクロム融合型グルコースデヒドロゲナーゼ及びグルコース測定法
KR20240133718A (ko) 재조합 발현 글루타민산 옥시다아제
JP6282115B2 (ja) 熱安定性が向上したアマドリアーゼ、その遺伝子および組換えｄｎａならびに熱安定性が向上したアマドリアーゼの製造法
KR20250099140A (ko) Fad형 글루타민산 디히드로게나제
WO2016063984A1 (ja) デヒドロゲナーゼ活性の向上したアマドリアーゼ
JPWO2017183717A1 (ja) ＨｂＡ１ｃデヒドロゲナーゼ
JP6398295B2 (ja) 変異型グルコース−６−リン酸脱水素酵素
WO2012043601A1 (ja) アマドリアーゼ改変体
CN108342370B (zh) 一种新型果糖基肽氧化酶及其应用
JP6504390B2 (ja) 改変型ピラノース酸化酵素
JP6764219B2 (ja) グッド緩衝液に対して安定なアマドリアーゼ
WO2025178134A1 (ja) Ｎａｄｈオキシダーゼ、その変異体、凍結乾燥標品、それを含むキット若しくは電極、電極を備えたセンサ、及びｎａｄｈの測定方法
JP2014143942A (ja) ジアホラーゼ組成物
WO2024158050A1 (ja) ルシフェラーゼ変異体
WO2018230304A1 (ja) 改変型グルコース脱水素酵素
JP2014180223A (ja) アルドヘキソースデヒドロゲナーゼ及びその製造方法

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20250428

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application