KR20250025516A - 포유류 세포의 배양 방법 - Google Patents

포유류 세포의 배양 방법 Download PDF

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Publication number
KR20250025516A
KR20250025516A KR1020257004730A KR20257004730A KR20250025516A KR 20250025516 A KR20250025516 A KR 20250025516A KR 1020257004730 A KR1020257004730 A KR 1020257004730A KR 20257004730 A KR20257004730 A KR 20257004730A KR 20250025516 A KR20250025516 A KR 20250025516A
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KR
South Korea
Prior art keywords
cells
concentration
poloxamer
pore
days
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Pending
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KR1020257004730A
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English (en)
Korean (ko)
Inventor
크리스토퍼 황
티모시 존슨
제이슨 왈터
쳉 쳉
조나단 왕
네하 샤
슬-아 배
Original Assignee
젠자임 코포레이션
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Publication of KR20250025516A publication Critical patent/KR20250025516A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020257004730A 2014-12-22 2015-12-21 포유류 세포의 배양 방법 Pending KR20250025516A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462095734P 2014-12-22 2014-12-22
US62/095,734 2014-12-22
KR1020237027804A KR102769649B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법
PCT/US2015/067040 WO2016106192A2 (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020237027804A Division KR102769649B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법

Publications (1)

Publication Number Publication Date
KR20250025516A true KR20250025516A (ko) 2025-02-21

Family

ID=55527619

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020257004730A Pending KR20250025516A (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법
KR1020177020466A Active KR102568951B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법
KR1020237027804A Active KR102769649B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020177020466A Active KR102568951B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법
KR1020237027804A Active KR102769649B1 (ko) 2014-12-22 2015-12-21 포유류 세포의 배양 방법

Country Status (14)

Country Link
US (2) US11306341B2 (https=)
EP (1) EP3237604B1 (https=)
JP (4) JP6843750B2 (https=)
KR (3) KR20250025516A (https=)
CN (1) CN107567495A (https=)
AU (3) AU2015369809B2 (https=)
BR (1) BR112017013423B1 (https=)
CA (2) CA3225136A1 (https=)
IL (2) IL253071B2 (https=)
MX (2) MX383857B (https=)
RU (1) RU2712746C2 (https=)
SG (2) SG11201705103WA (https=)
TW (3) TWI751103B (https=)
WO (1) WO2016106192A2 (https=)

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TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
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Publication number Publication date
AU2015369809B2 (en) 2022-03-17
US20160177361A1 (en) 2016-06-23
IL308064A (en) 2023-12-01
JP6843750B2 (ja) 2021-03-17
KR102568951B1 (ko) 2023-08-22
AU2025201416A1 (en) 2025-03-20
JP2017538435A (ja) 2017-12-28
JP2024050754A (ja) 2024-04-10
RU2017126045A (ru) 2019-01-24
IL253071B1 (en) 2023-12-01
JP2021019624A (ja) 2021-02-18
IL253071A0 (en) 2017-08-31
MX2017008338A (es) 2017-10-19
JP2022107000A (ja) 2022-07-20
CN107567495A (zh) 2018-01-09
MX2021007433A (es) 2021-08-05
WO2016106192A2 (en) 2016-06-30
TW202200785A (zh) 2022-01-01
TWI741851B (zh) 2021-10-01
TW202106706A (zh) 2021-02-16
IL253071B2 (en) 2024-04-01
MX383857B (es) 2025-03-14
KR102769649B1 (ko) 2025-02-19
AU2022204152A1 (en) 2022-06-30
TWI843012B (zh) 2024-05-21
BR112017013423B1 (pt) 2023-04-11
SG11201705103WA (en) 2017-07-28
CA3225136A1 (en) 2016-06-30
TWI751103B (zh) 2022-01-01
US12486524B2 (en) 2025-12-02
WO2016106192A3 (en) 2016-11-03
KR20230125102A (ko) 2023-08-28
CA2971861A1 (en) 2016-06-30
TW201639881A (zh) 2016-11-16
BR112017013423A2 (pt) 2018-01-09
RU2712746C2 (ru) 2020-01-30
AU2015369809A1 (en) 2017-08-10
US20220145348A1 (en) 2022-05-12
CA2971861C (en) 2024-02-13
US11306341B2 (en) 2022-04-19
JP7075463B2 (ja) 2022-05-25
EP3237604A2 (en) 2017-11-01
EP3237604B1 (en) 2026-02-11
RU2017126045A3 (https=) 2019-06-24
AU2022204152B2 (en) 2024-11-28
HK1246345A1 (zh) 2018-09-07
RU2020101112A (ru) 2020-03-13
SG10202001034PA (en) 2020-04-29
KR20170090508A (ko) 2017-08-07

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Comment text: Divisional Application for International Patent

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Patent event date: 20250213

Application number text: 1020237027804

Filing date: 20230816

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