KR20230116864A - Lca 및 3-kca를 udca 및 3-kudca로 전환하기 위한 효소적방법 - Google Patents
Lca 및 3-kca를 udca 및 3-kudca로 전환하기 위한 효소적방법 Download PDFInfo
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- KR20230116864A KR20230116864A KR1020237021971A KR20237021971A KR20230116864A KR 20230116864 A KR20230116864 A KR 20230116864A KR 1020237021971 A KR1020237021971 A KR 1020237021971A KR 20237021971 A KR20237021971 A KR 20237021971A KR 20230116864 A KR20230116864 A KR 20230116864A
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- leu
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/06—Hydroxylating
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
- C12Y114/14001—Unspecific monooxygenase (1.14.14.1)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063119188P | 2020-11-30 | 2020-11-30 | |
| US63/119,188 | 2020-11-30 | ||
| PCT/US2021/061025 WO2022115710A1 (en) | 2020-11-30 | 2021-11-29 | Enzymatic methods for converting lca and 3-kca to udca and 3-kudca |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20230116864A true KR20230116864A (ko) | 2023-08-04 |
Family
ID=81754944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020237021971A Pending KR20230116864A (ko) | 2020-11-30 | 2021-11-29 | Lca 및 3-kca를 udca 및 3-kudca로 전환하기 위한 효소적방법 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230416800A1 (https=) |
| EP (1) | EP4251169A4 (https=) |
| JP (1) | JP2023552528A (https=) |
| KR (1) | KR20230116864A (https=) |
| CN (1) | CN116670147A (https=) |
| AU (1) | AU2021385425A1 (https=) |
| CA (1) | CA3201311A1 (https=) |
| WO (1) | WO2022115710A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4665352A2 (en) * | 2023-02-14 | 2025-12-24 | Epimeron USA, Inc. | Recombinant polypeptides for biosynthesis of ursodeoxycholic acid (udca) |
| CN117025709A (zh) * | 2023-07-31 | 2023-11-10 | 华南理工大学 | 一种细胞色素p450酶联合细胞色素p450还原酶在合成熊去氧胆酸中的应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58155098A (ja) * | 1982-03-09 | 1983-09-14 | Yakult Honsha Co Ltd | 微生物によるウルソデオキシコ−ル酸の製造法 |
| US5989855A (en) * | 1996-03-29 | 1999-11-23 | Tokyo Tanabe Company Limited | Bile acid converting microorganism and process for preparing bile acid |
| WO2013086499A2 (en) * | 2011-12-09 | 2013-06-13 | Ceres, Inc. | Transgenic plants having altered biomass composition |
| EP3444340B1 (de) * | 2014-07-29 | 2020-04-15 | Pharmazell GmbH | 7beta-hydroxysteroid dehydrogenase-mutanten und verfahren zur herstellung von ursodesoxycholsäure |
| IT201900017411A1 (it) * | 2019-09-27 | 2021-03-27 | Ice S P A | Processo di preparazione di acido ursodesossicolico |
-
2021
- 2021-11-29 EP EP21899186.7A patent/EP4251169A4/en active Pending
- 2021-11-29 US US18/038,203 patent/US20230416800A1/en active Pending
- 2021-11-29 WO PCT/US2021/061025 patent/WO2022115710A1/en not_active Ceased
- 2021-11-29 KR KR1020237021971A patent/KR20230116864A/ko active Pending
- 2021-11-29 CA CA3201311A patent/CA3201311A1/en active Pending
- 2021-11-29 JP JP2023532459A patent/JP2023552528A/ja active Pending
- 2021-11-29 CN CN202180080103.1A patent/CN116670147A/zh active Pending
- 2021-11-29 AU AU2021385425A patent/AU2021385425A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA3201311A1 (en) | 2022-06-02 |
| CN116670147A (zh) | 2023-08-29 |
| US20230416800A1 (en) | 2023-12-28 |
| JP2023552528A (ja) | 2023-12-18 |
| AU2021385425A9 (en) | 2025-01-09 |
| WO2022115710A1 (en) | 2022-06-02 |
| EP4251169A1 (en) | 2023-10-04 |
| AU2021385425A1 (en) | 2023-06-15 |
| EP4251169A4 (en) | 2025-07-09 |
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| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
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| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
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| A201 | Request for examination | ||
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
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| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
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| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
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| D21 | Rejection of application intended |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE) |
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| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |