JP2023552528A - Ｌｃａおよび３－ｋｃａをｕｄｃａおよび３－ｋｕｄｃａに変換するための酵素的方法 - Google Patents

Ｌｃａおよび３－ｋｃａをｕｄｃａおよび３－ｋｕｄｃａに変換するための酵素的方法 Download PDF

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JP2023552528A
JP2023552528A JP2023532459A JP2023532459A JP2023552528A JP 2023552528 A JP2023552528 A JP 2023552528A JP 2023532459 A JP2023532459 A JP 2023532459A JP 2023532459 A JP2023532459 A JP 2023532459A JP 2023552528 A JP2023552528 A JP 2023552528A
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seq
nucleic acid
acid sequence
enzyme
cyp
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JP2023552528A5 (https=
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リード，ジェイ．，グレゴリー
レッディ，ジャヤチャンドラ，ピー．
ポール，バ－ナード，ジェイ．
シェル，ウルスラ
グレゴリー，マット
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サンドヒル ワン，エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001—Unspecific monooxygenase (1.14.14.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2023532459A 2020-11-30 2021-11-29 Ｌｃａおよび３－ｋｃａをｕｄｃａおよび３－ｋｕｄｃａに変換するための酵素的方法 Pending JP2023552528A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063119188P 2020-11-30 2020-11-30
US63/119,188 2020-11-30
PCT/US2021/061025 WO2022115710A1 (en) 2020-11-30 2021-11-29 Enzymatic methods for converting lca and 3-kca to udca and 3-kudca

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JP2023552528A true JP2023552528A (ja) 2023-12-18
JP2023552528A5 JP2023552528A5 (https=) 2024-12-06

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JP2023532459A Pending JP2023552528A (ja) 2020-11-30 2021-11-29 Ｌｃａおよび３－ｋｃａをｕｄｃａおよび３－ｋｕｄｃａに変換するための酵素的方法

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US (1) US20230416800A1 (https=)
EP (1) EP4251169A4 (https=)
JP (1) JP2023552528A (https=)
KR (1) KR20230116864A (https=)
CN (1) CN116670147A (https=)
AU (1) AU2021385425A1 (https=)
CA (1) CA3201311A1 (https=)
WO (1) WO2022115710A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4665352A2 (en) * 2023-02-14 2025-12-24 Epimeron USA, Inc. Recombinant polypeptides for biosynthesis of ursodeoxycholic acid (udca)
CN117025709A (zh) * 2023-07-31 2023-11-10 华南理工大学 一种细胞色素p450酶联合细胞色素p450还原酶在合成熊去氧胆酸中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58155098A (ja) * 1982-03-09 1983-09-14 Yakult Honsha Co Ltd 微生物によるウルソデオキシコ−ル酸の製造法
WO1997036997A1 (en) * 1996-03-29 1997-10-09 Tokyo Tanabe Company Limited Novel bile acid converting microorganism and process for preparing bile acid

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013086499A2 (en) * 2011-12-09 2013-06-13 Ceres, Inc. Transgenic plants having altered biomass composition
EP3444340B1 (de) * 2014-07-29 2020-04-15 Pharmazell GmbH 7beta-hydroxysteroid dehydrogenase-mutanten und verfahren zur herstellung von ursodesoxycholsäure
IT201900017411A1 (it) * 2019-09-27 2021-03-27 Ice S P A Processo di preparazione di acido ursodesossicolico

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58155098A (ja) * 1982-03-09 1983-09-14 Yakult Honsha Co Ltd 微生物によるウルソデオキシコ−ル酸の製造法
WO1997036997A1 (en) * 1996-03-29 1997-10-09 Tokyo Tanabe Company Limited Novel bile acid converting microorganism and process for preparing bile acid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SASCHA GROBE ET AL.: ""Engineering Regioselectivity of a P450 Monooxygenase Enables the Synthesis of Ursodeoxycholic Acid", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 60, no. 2, JPN6025045240, 12 November 2020 (2020-11-12), pages 753 - 757, ISSN: 0005723030 *
SHANHUI XU ET AL.: ""Yeast as a promising heterologous host for steroid bioproduction"", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. Vol. 47, No. 9-10, JPN6025045239, 1 October 2020 (2020-10-01), pages 829 - 843, ISSN: 0005723031 *
WEI LU ET AL.: ""Distinct Regioselectivity of Fungal P450 Enzymes for Steroidal Hydroxylation"", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 85, no. 18, JPN6025045238, 15 September 2019 (2019-09-15), ISSN: 0005723032 *

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Publication number Publication date
CA3201311A1 (en) 2022-06-02
CN116670147A (zh) 2023-08-29
US20230416800A1 (en) 2023-12-28
AU2021385425A9 (en) 2025-01-09
WO2022115710A1 (en) 2022-06-02
KR20230116864A (ko) 2023-08-04
EP4251169A1 (en) 2023-10-04
AU2021385425A1 (en) 2023-06-15
EP4251169A4 (en) 2025-07-09

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