CN115243701A - 用于无佐剂诱导免疫应答的IgG变体 - Google Patents

用于无佐剂诱导免疫应答的IgG变体 Download PDF

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CN115243701A
CN115243701A CN202180015476.0A CN202180015476A CN115243701A CN 115243701 A CN115243701 A CN 115243701A CN 202180015476 A CN202180015476 A CN 202180015476A CN 115243701 A CN115243701 A CN 115243701A
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H·梅森
A·黛盟斯
M·帕德赫
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Abstract

本公开涉及可用于设计针对多种病原体的疫苗接种的免疫球蛋白变体及其生产和使用方法。在一些方面，免疫球蛋白变体是IgG(例如6D8抗体)的变体。

Description

用于无佐剂诱导免疫应答的IgG变体
相关申请
本申请要求于2020年2月21日提交的题为“用于无佐剂诱导中和免疫应答的IgG变体”的美国临时专利申请62/980,012的权益，其全部公开内容通过引用并入本文。
以电子方式提交的材料通过引用并入
通过引用将其全部并入本文中的是与本文同时提交的计算机可读核苷酸/氨基酸序列表，其标识如下：一个名为“SeqList”的416,572字节ASCII(文本)文件，创建于2021年2月21日。
技术领域
本公开涉及无佐剂诱导有效免疫应答的免疫球蛋白变体和用于在植物中产生此类变体的载体。
背景技术
由重组蛋白抗原组成的亚单位疫苗由于其安全性、易于生产和引发针对期望表位的靶向免疫应答的能力而非常有前景。然而当递送它们自身时，这些抗原通常无法生成强大而持久的免疫应答，使得需要采取增强其免疫原性的策略。因此，重组蛋白抗原已成为治疗用途的候选物。与免疫球蛋白Fc结构域的蛋白质融合已显示出作为治疗候选物的巨大潜力。感兴趣的蛋白质与Fc融合可以增强融合伴侣的溶解度和稳定性，同时还允许通过蛋白质A/G亲和层析进行简单且经济高效的纯化。此外，通过与体内的新生儿Fc受体(FcRn)相互作用，Fc融合体可以避免溶酶体降解，从而延长Fc融合体的血清半衰期。
Fc融合体的大部分工作集中在提高其治疗潜力上，而很少有研究探讨Fc融合体作为增强抗原免疫原性的策略。含有Fcγ受体和补体受体C1q的抗原呈递细胞可以摄取和处理结合IgG的抗原。然而，这些相互作用需要高亲和力结合以激活，因此单价Fc-抗原融合体不能有效地利用这些途径。另一方面，具有多价Fc结构域的较大抗原-抗体免疫复合物可以交联Fc受体并有效结合C1q，导致大大改善通过树突状细胞摄取和呈递、以及改善T细胞的活化。通过将抗体与抗原混合而生成的免疫复合物通常产生不一致的结果：免疫应答可能偏向有利的抗原位点，但整体免疫原性可能没有显著提高。
相比之下，重组免疫复合物(RIC)已用于生产针对破伤风梭菌(Clostridiumtetani)、埃博拉病毒、结核分枝杆菌(Mycobacterium tuberculosis)、登革热病毒和人乳头瘤病毒的候选疫苗。RIC是一种与其同源抗原基因融合的IgG，它允许形成较大的高免疫原性抗原-抗体簇，模拟天然感染期间发现的那些抗原-抗体簇。
发明内容
本公开涉及作为免疫球蛋白变体的重组蛋白，例如免疫球蛋白G(IgG)变体，及其生产和使用方法。在一些方面，IgG变体基于6D8抗体。在重组蛋白中的抗体部分是6D8抗体的某些实施方案中，包含抗体的CH2结构域和CH3结构域的Fc融合体包含置换突变E345R、E430G和S440Y。
在一些实施方案中，重组蛋白包含抗原单元、包含IgG的CH2结构域和CH3结构域的Fc融合体单元、IgG的可变重链(VH)结构域单元、IgG的CH1结构域单元；和IgG的轻链可变(VL)结构域单元。抗原单元不是IgG的表位，它在N-末端与IgG的VH结构域单元连接或在C-末端与IgG的CH3结构域连接。IgG的CH1结构域单元与IgG的CH2结构域连接，而IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域融合。在一些实施方案方案中，重组蛋白进一步包含表位标签单元，其中表位标签是IgG的表位。
在一些方面，重组蛋白包含两个抗原单元、两个Fc融合体单元、两个IgG的CH1结构域单元、两个IgG的VH结构域单元和两个IgG的VL结构域单元。在IgG的CH2结构域和CH1结构域的连接处形成的二硫键连接两个Fc融合体单元。在一些方面，每个IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域连接。在特定实施方案中，重组蛋白不包含IgG的任何轻链恒定(CL)结构域。在重组蛋白的一些实施方案中，重组蛋白包含两个抗原单元、两个Fc融合体单元、两个IgG的CH1结构域单元、两个IgG的VH结构域单元和两个IgG的轻链可变(VL)结构域单元，重组蛋白进一步包含两个表位标签单元，其中表位标签是IgG的表位。在一些实施方案中，两个表位标签单元在IgG的CH3结构域的C-末端与两个Fc融合体单元连接，而两个抗原单元与两个IgG的VH结构域单元连接。在其他实施方案中，两个表位标签单元与两个抗原单元连接，而两个抗原单元与IgG的CH3结构域的C-末端处的两个Fc融合体单元连接。在具体的实施方案中，抗体是6D8抗体，表位标签包含肽序列YKLDIS(SEQ IDNO.1)。
在其他实施方案中，重组蛋白包含抗原单元、包含IgG的CH2结构域和CH3结构域的Fc融合体单元、IgG的VH结构域单元、IgG的CH1结构域单元、IgG的VL结构域单元；和IgG的CL结构域单元。在此类实施方案中，IgG是6D8抗体，Fc融合体包含置换突变E345R、E430G和S440Y。抗原单元不是6D8抗体的表位，它在C-末端与IgG的CH3结构域连接。IgG的VL结构域单元与IgG的CL结构域单元连接，IgG的CL结构域单元与IgG的CH1结构域单元连接。然后将IgG的CH1结构域单元与IgG的CH2结构域连接。在一些方面，重组蛋白进一步包含用于6D8抗体的表位标签，例如包含肽序列YKLDIS(SEQ ID NO.1)。
在一些方面，重组蛋白中的表位标签包含序列VYKLDISEA(SEQ ID NO.2)。在其他方面，表位标签由序列YKLDIS(SEQ ID NO.1)组成。
在重组蛋白的另外其它实施方案中，IgG变体包含两个抗原单元、两个Fc融合体单元、两个IgG的VH结构域单元和两个IgG的CH1结构域单元。抗原不是IgG的表位，两个抗原单元在N-末端与两个IgG的VH结构域单元连接。两个IgG的CH1结构域单元与两个Fc融合体单元在IgG的CH2结构域处连接。在一些方面，重组蛋白不包含IgG的CL结构域并且不包含IgG的VL结构域。
在某些实施方案中，重组蛋白的抗原来自寨卡病毒，例如，抗原单元包含登录号AMC13911.1的K301-T406。在其他实施方案中，重组蛋白的抗原来自诺如病毒，例如，抗原单元包含来自诺如病毒主要衣壳蛋白的至少一部分和/或来自诺如病毒非结构蛋白1(NS1)的至少一部分。在一些实施方案中，抗原单元包含来自诺如病毒主要衣壳蛋白的突出结构域(protruding domain)、诺如病毒主要衣壳蛋白的壳结构域(shell domain)或来自诺如病毒NS1的至少一个5至500个残基长的部分。在一些方面，重组蛋白的抗原可以包含多种抗原肽。例如，在一些实施方案中，抗原单元包含来自多个诺如病毒毒株或物种(例如来自人诺如病毒GI.3、人诺如病毒GII.4和鼠诺如病毒(MNV))的主要衣壳蛋白的至少一部分或NS1的至少一部分。在一些实施方案中，抗原单元包含至少一个在SEQ ID NO.34-72中列出的序列。
本文所述的生产方法包括在植物中表达重组蛋白。换言之，重组蛋白是在转基因植物中产生的。在某些实施方案中，重组蛋白在木糖基转移酶和岩藻糖基转移酶被沉默的转基因植物中表达。在一些方面，生产本文所述的重组蛋白的方法包括将选自以下的载体引入农杆菌：pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、pBYKEMd-HZE3、pBYKEMd-ZE3H、pBYKEMd-ZE3Hx、pBYKEMd-HVLZe、pBYKEMd2-HVL-Hx、pBYKEMd2-HVLZnt、pBYKEMd2-ZHVLnt、pBYKEMd2-ZHVLe、pBYKEMd2-ZHVLhx、pBYKEAM-N12MHd、pBYKEAM-NPHd、pBYKEAM-NSHd、pBYKEAM-NTHd、pBYKEHM-CPHd、pBYKEHM-CSHd、和pBYKEHM-CTHd，并用含有所述载体的农杆菌渗入植物部分以产生转化的植物部分。然后从转化的植物中提取粗蛋白，然后纯化得到重组蛋白。对于其中引入农杆菌的载体选自pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、和pBYKEMd-HZE3的生产方法，所述方法进一步包括用含有pBYKEMd-6D8K的农杆菌与含有所述载体的农杆菌共渗入。
本文所述的使用方法包括在受试者中诱导针对重组蛋白中的抗原的免疫应答的方法。所述方法包括将所述重组蛋白施用于受试者。在某些实施方案中，所述方法在受试者中诱导针对寨卡病毒的免疫应答。在此类实施方案中，重组蛋白包含具有包含登录号AMC13911.1的K301-T406的氨基酸序列的抗原。在其他实施方案中，所述方法在受试者中诱导针对诺如病毒(包括人诺如病毒和MNV)的免疫应答。在此类实施方案中，重组蛋白包含来自诺如病毒VP1的抗原，例如来自其突出结构域或壳结构域、或来自诺如病毒NS1的抗原。
在一些实施方案中，向受试者施用组合物，所述组合物包含具有来自多种诺如病毒的VP1抗原的重组蛋白和具有来自多种诺如病毒的NS1抗原的重组蛋白。在另外其他实施方案中，重组蛋白在转基因植物中产生。在一些方面，转基因植物的木糖基转移酶和岩藻糖基转移酶被沉默。
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图1A-图1B根据某些实施方案描绘了IgG融合构建体和其各自结合Clq的能力比较。图1A是本文公开的某些IgG融合构建体的示意图。如该图中所用，ZE3是指包含氨基酸K301至T406的寨卡病毒包膜结构域III；e是指具有由VYKLDISEA(SEQ ID NO.2)组成的肽序列的表位标签，该序列是重组免疫复合物(RIC)形成的6D8结合基序；带有闪电的e是指具有由YKLDIS(SEQ ID NO.1)组成的肽序列的表位标签，以减少RIC形成；VH是指可变重结构域；VL是指可变轻结构域；H是指重链恒定CH1结构域；L是指轻链恒定结构域；Fc是指重链恒定区的一部分，其由CH2和CH3结构域组成；带有闪电的Fc是指与Fc相同但具有E345R、E430G和S440Y突变以诱导六聚体形成。图1B描绘了纯化的IgG融合构建体的C1q结合ELISA，其中IgG融合构建体构建于mAb 6D8人IgG1主链上。用10μg/ml人C1q包被ELISA板，并用10μg/ml的每种融合体孵育，使用非融合体的6D8抗体用作阴性对照。使用多克隆山羊抗人IgG-HRP检测构建体。三个样品的平均OD450值显示为±标准误差，如通过单因素方差分析(one-wayANOVA)在指示的组之间进行比较所测量的，一个星号(*)表示p<0.05，三个星号(***)表示p<0.001。
图2A-图2B根据某些实施方案描绘了构建于6D8抗体主链上的IgG融合体的表达。图2A描绘了IgG融合构建体表达的ELISA和凝胶定量。通过ELISA或SDS-PAGE然后通过凝胶定量，分析了来自叶斑的澄清蛋白质提取物，所述叶斑已用农杆菌渗入每种IgG融合构建体。对于ZE3 ELISA，板用多克隆小鼠抗ZE3包被，用每个IgG融合体提取物的连续稀释液孵育，使用纯化的HLZ作为标准品，并用山羊抗人IgG-HRP探测。对于IgG ELISA，用提取物的连续稀释液或人IgG标准品包被板，并用山羊抗人IgG-HRP探测。对于凝胶定量，ImageJ软件用于比较在无染色聚丙烯酰胺凝胶上可视化的IgG融合体条带强度，纯化的6D8用作标准品。柱代表来自三个独立渗入的叶样品的平均值±标准误差。图2B描绘了通过还原SDS-PAGE分离的澄清叶提取物的代表性凝胶图像。对应于各个重链/ZE3融合体的条带位置表示为“ZH/HZ”。HLZe和HLZd大小的微小变化是由于表位标签的存在。显示了Rubisco的大亚基“RbcL”以及ZFc和Fc条带。
图3根据某些实施方案描绘了构建于6D8抗体主链上的IgG融合体的纯化。对农杆菌渗入的叶材料进行匀浆、澄清和通过蛋白G亲和层析纯化，每个构建体的所述叶材料来自1-3株植物。合并峰洗脱液并使用无染色聚丙烯酰胺凝胶在非还原和还原SDS-PAGE上分离。显示了每个构建体的代表性泳道。
图4A-图4B根据某些实施方案描绘了构建于6D8抗体主链上的IgG融合体的蔗糖梯度密度离心。通过蔗糖梯度离心，使用5/10/15/20/25％不连续蔗糖层分离纯化的IgG融合体。通过SDS-PAGE分析梯度级分，并显示了代表性结果。使用ImageJ软件定量相对条带强度，并任意指定峰条带的值为1。
图5A-图5B根据某些实施方案描绘了纯化的构建于6D8抗体主链上的IgG融合体的稳定性和C1q结合。纯化的IgG融合体样品在纯化后冷冻和解冻一次(初始)，或另外进行5次冷冻/解冻循环、或在4℃孵育2周、或在室温孵育2周。图5A显示了每种处理的完全组装产品的相对比例(使用ImageJ软件分析)。处理后，在还原和非还原SDS-PAGE凝胶上分离样品。图5B显示了每种处理后IgG构建体的Clq结合ELISA结果。柱代表来自三个样品的平均OD450值±标准误差。
图6A-图6B根据某些实施方案描绘了用IgG融合体免疫小鼠的结果和血清滴度。皮下免疫BALB/c小鼠(每组6只)两次，间隔两周，给药剂量为每种IgG融合体将递送8μg ZE3，或以PBS作为对照。在最终给药后两周收集小鼠血清样品。对于图6A，通过ELISA分析连续稀释的小鼠血清的总IgG产生。终点取为给出的OD450读数至少为背景两倍的最大稀释度的倒数。通过方差分析将指示的列与ZE3比较，三个星号(***)表示p<0.01。对于图6B，将小鼠血清样品1:100稀释并通过ELISA分析IgG2a的产生。通过方差分析将指示的列与HLZ比较，(**)表示p<0.05，(***)表示p<0.01。
图7根据某些实施方案描绘了单链抗体(HVL)和HVL相关构建体。所示单链抗体含有与可变轻链区连接的6D8抗体重链可变区，所述可变轻链区直接融合到6D8抗体重链上。如该图中所用，ZE3是指含有氨基酸K301至T406的寨卡病毒包膜结构域III；e是指具有由VYKLDISEA(SEQ ID NO.2)组成的肽序列的表位标签，该序列是用于RIC形成的6D8结合基序；VH是指来自6D8抗体的可变重结构域；VL是指来自6D8抗体的可变轻结构域；H是指来自6D8抗体的重链恒定区CH1结构域；Fc是指由来自6D8抗体的CH2和CH3结构域组成的重链恒定区；带有闪电的Fc是指与Fc相同但具有E345R、E430G和S440Y突变以诱导六聚体形成。
图8根据某些实施方案描绘了HVL和HVL-融合构建体的纯化和蛋白质印迹分析。在蛋白G纯化HVL、HVLZe和ZHVLHx构建体后，在4-15％聚丙烯酰胺无染色凝胶上分离来自峰洗脱的样品。通过蛋白质印迹进行HVLHX和HVLZnt构建体的分析，所述蛋白质印迹含有已通过离心澄清的小规模叶样品。将样品在4-15％聚丙烯酰胺凝胶上分离，转移到PVDF膜上，并用HRP标记的山羊抗人IgG抗体检测。
图9根据某些实施方案描绘了野生型和糖工程化(glycoengineered)植物产生的IgG融合构建体的C1q结合比较。用10μg/ml人C1q包被ELISA板，并用5μg/ml的每种纯化构建体孵育。使用多克隆山羊抗人IgG-HRP检测构建体。三个重复的平均OD450值显示为±标准误差，如通过单因素方差分析在指示的组之间进行比较所测量的，两个星号表示p<0.01。如该图中所用，wt是指在野生型本氏烟草(Nicotiana benthamiana)植物中制造的构建体，而GnGn是指在木糖基转移酶和岩藻糖基转移酶被沉默的糖工程化植物中制造的构建体。
图10根据某些实施方案描绘了构建于6D8抗体主链上的IgG融合体之间的Clq结合比较。用10μg/ml人C1q包被ELISA板，并用从50μg/ml开始的每种纯化构建体的10倍连续稀释液孵育。使用多克隆山羊抗人IgG-HRP检测构建体。显示了三次重复的平均OD450值。如该图中所用，wt是指在野生型本氏烟草植物中制造的构建体；GnGn是指在木糖基转移酶和岩藻糖基转移酶被沉默的糖工程化植物中制造的构建体；Ag是指用6D8表位标签化的登革热共有登革热E蛋白结构域III(cE)；ZHL是指与HL相同但ZE3与6D8重链的N-末端融合。
图11根据某些实施方案描绘了将构建于6D8抗体主链(HVL)上的单链抗体的表位结合与野生型6D8抗体的表位结合进行比较。用900ng纯化的表位标签化蛋白包被ELISA板，用连续稀释的HVL(可变重链与融合到6D8重链的可变轻链区连接的单链抗体)或全长6D8抗体孵育板。
图12根据某些实施方案描绘了显示RIC不溶性的代表性SDS-PAGE。从用指定构建体农杆菌渗入的本氏烟草的叶中提取蛋白质，并在非还原条件下通过SDS-PAGE分离然后进行蛋白质印迹。使用山羊抗人IgG-HRP检测构建体。可溶(S)是指经澄清的叶粗提物，而不溶(IS)是指澄清后的沉淀物，其重悬浮于SDS样品缓冲液中。
图13A-图13C根据某些实施方案描绘了对6D8表位标签突变体的溶解度和结合的研究。对于图13A-图13B，从用指定构建体农杆菌浸润的本氏烟草的叶子中提取蛋白质，并在非还原条件下通过SDS-PAGE分离，然后使用山羊抗人IgG-HRP作为探针进行蛋白质印迹。可溶是指经澄清的叶粗提物，而不溶是指澄清后的沉淀物，其重悬浮于SDS样品缓冲液中。命名为“a”的表位突变体具有由VYKLDISEA(SEQ ID NO.2)组成的肽序列。命名为“b”的表位突变体具有由VYKLDISE(SEQ ID NO.3)组成的肽序列。命名为“c”的表位突变体具有由YKLDISE(SEQ ID NO.4)组成的肽序列。命名为“d”的表位突变体具有由YKLDIS(SEQ IDNO.1)组成的肽序列。对于图13C，用全尺寸6D8随后用山羊抗人κ-HRP探测用纯化重链的连续稀释液包被的ELISA板，所述连续稀释液含有指定的表位标签突变体。平均OD450值显示为三次重复±标准误差。
图14A和14B分别根据某些实施方案描绘了靶向诺如病毒(NoV)的重组免疫复合物(RIC)构建体的示意图和纯化的RIC构建体的SDS-PAGE。在图14B中，靶向VP1突出结构域的保守表位的RIC构建体标记为P-RIC(抗原单元包含串联连接的序列SEQ ID NO.34-42)；VP1壳结构域，标记为S-RIC(抗原单元包含串联连接的序列SEQ ID NO.45-53)；T细胞表位，标记为T-RIC(抗原单元包含串联连接的序列SEQ ID NO.58-72)；并且含有抗原单元，其包含来自人诺如病毒GI.3、人诺如病毒GII.4和鼠诺如病毒(MNV)的非结构蛋白1(NS1)的表位，标记为N-RIC(抗原单元包含串联连接的序列SEQ ID NO.55-57)。将RIC纯化，在还原条件下通过SDS-PAGE分离，并在UV下通过无染色2,2,2-三氯乙醇(TCE)成像可视化。“+抑制剂”是指加入植物蛋白酶抑制剂混合物(cocktail)以减少N-RIC的降解。
图15根据某些实施方案描绘了靶向诺如病毒的纯化RIC构建体的SDS-PAGE(左)和蛋白质印迹(右)。纯化的RIC在非还原(NR)或还原(R)条件下通过SDS-PAGE分离，并在UV下通过无染色2,2,2-三氯乙醇(TCE)成像可视化(左)。用抗鼠诺如病毒1(MNV)NS1/2抗体或抗MNV衣壳抗体进行蛋白质印迹。P，P-RIC；S，S-RIC；T，T-RIC；N-RIC。
图16根据某些实施方案描绘了与鼠诺如病毒结合的血清。用3剂通过RIC构建体递送的2μg总抗原、或人诺如病毒GI.I(诺瓦克病毒)/人诺如病毒GII.4(Minerva病毒)混合的VLP(标记为“VLP”)免疫小鼠，通过ELISA测试来自所述小鼠的血清与鼠诺如病毒1(MNV-1)病毒的结合。聚苯乙烯96孔板用兔抗MNV衣壳包被，用5％PBSTM封闭，然后用感染MNV-1的RAW267.4细胞的裂解物孵育。孵育后，将小鼠血清的连续稀释液加入板，然后用山羊抗小鼠IgG-HRP偶联物检测。显示了含有两个技术重复的合并小鼠血清的平均OD450值±标准误差。缩写：P-RIC：用P-RIC免疫的小鼠的血清；S-RIC：用S-RIC免疫的小鼠的血清；T-RIC：用T-RIC免疫的小鼠的血清；N-RIC：用N-RIC免疫的小鼠的血清；PSTN-RIC：用P-RIC、S-RIC、T-RIC和N-RIC(但剂量为单个实验所用剂量的四分之一)免疫的小鼠的血清。
图17根据某些实施方案描述了血清与GI.I VLP结合。用3剂通过RIC构建体递送的2μg总抗原免疫小鼠，通过ELISA测试所述小鼠的血清与GI.I诺如病毒VLP的结合。聚苯乙烯96孔板用植物制造的诺瓦克病毒VLP孵育，封闭，然后用小鼠血清的系列稀释液孵育。用山羊抗小鼠IgG-HRP偶联物检测结合的抗体。显示了含有两个技术重复的六个样品的平均OD450值±标准误差。缩写：P-RIC：用P-RIC免疫的小鼠的血清；S-RIC：用S-RIC免疫的小鼠的血清；T-RIC：用T-RIC免疫的小鼠的血清；N-RIC：用N-RIC免疫的小鼠的血清；PSTN-RIC：用P-RIC、S-RIC、T-RIC和N-RIC(但剂量为单个实验所用剂量的四分之一)免疫的小鼠的血清。
图18根据某些实施方案描绘了与人诺如病毒GII.2VLP结合的血清。用3剂通过RIC构建体递送的2μg总抗原免疫小鼠，通过ELISA测试所述小鼠的血清与人诺如病毒GII.2(雪山病毒)VLP的结合。聚苯乙烯96孔板用植物制造的GII.2VLP孵育，封闭，然后用小鼠血清的系列稀释液孵育。用山羊抗小鼠IgG-HRP偶联物检测结合的抗体。显示了含有两个技术重复的合并血清样品的平均OD450值±标准误差。缩写：P-RIC：用P-RIC免疫的小鼠的血清；S-RIC：用S-RIC免疫的小鼠的血清；T-RIC：用T-RIC免疫的小鼠的血清；N-RIC：用N-RIC免疫的小鼠的血清；PSTN-RIC：用P-RIC、S-RIC、T-RIC和N-RIC(但剂量为单个实验所用剂量的四分之一)免疫的小鼠的血清。
图19根据某些实施方案描绘了用靶向诺如病毒的VLP或RIC构建体免疫的小鼠产生IFN-γ。从免疫的小鼠中收获脾细胞并用其相应抗原刺激72小时。收集来自受刺激和未受刺激的脾细胞的上清液，通过ELISA测定IFN-γ的产生。聚苯乙烯96孔板用连续稀释的脾细胞上清液或重组小鼠IFN-γ标准品包被，用5％PBSTM封闭，然后用大鼠抗小鼠IFN-γ和山羊抗大鼠IgG辣根过氧化物酶偶联物进行探测。OD450值用于构建标准曲线，并显示了六个样品的平均IFN-γ产生±标准误差，每个样品含有两个技术重复。缩写：P-RIC：用P-RIC免疫的小鼠的血清；S-RIC：用S-RIC免疫的小鼠的血清；T-RIC：用T-RIC免疫的小鼠的血清；N-RIC：用N-RIC免疫的小鼠的血清；PSTN-RIC：用P-RIC、S-RIC、T-RIC和N-RIC(但剂量为单个实验所用剂量的四分之一)免疫的小鼠的血清。
图20-图39描绘了pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、pBYKEMd-HZE3、pBYKEMd-ZE3H、pBYKEMd-ZE3Hx、pBYKEMd-HVLZe、pBYKEMd2-HVL-Hx、pBYKEMd2-HVLZnt、pBYKEMd2-ZHVLnt、pBYKEMd2-ZHVLe、pBYKEMd2-ZHVLhx、pBYKEMd-6D8K、pBYKEAM-N12MHd、pBYKEAM-NPHd、pBYKEAM-NSHd、pBYKEAM-NTHd、pBYKEHM-CPHd、pBYKEHM-CSHd、和pBYKEHM-CTHd的图谱。
具体实施方式
本公开的详细方面和应用在以下附图和具体实施方案中描述。除非特别指出，否则旨在对说明书和权利要求书中的词语和短语赋予对于适用领域的普通技术人员而言简单、普通和习惯的含义。
在以下描述中，并且出于解释的目的，阐述了许多具体细节以便提供对本公开的各个方面的透彻理解。然而，相关领域的技术人员将理解，可以在没有这些具体细节的情况下实践本文公开的技术的实施方案。应当注意的是，有许多不同的和可选的配置、设备和技术可以应用所公开的技术。本文公开的技术的全部范围不限于以下描述的实施例。
单数形式“一”、“一个”和“所述……”包括复数所指对象，除非上下文另有明确规定。因此，例如，提及“一个步骤”包括提及一个或多个此类步骤。
如本文所用，术语“6D8抗体”是指针对埃博拉病毒GP1蛋白的单克隆抗体(描述于Wilson等人，2000中；植物优化序列描述于Huang等人，2010中)。在一些实施方案中，6D8抗体的CH1结构域是指包含TKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVS
WNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC(SEQID NO.5)的肽序列，或具有至少80％、至少85％、至少90％、至少95％、至少98％、或至少99％序列相似性的肽序列。在一些实施方案中，6D8抗体的CH2结构域是指包含DKTHTCPPCPAPELLGGPSVFLFPPKP
KDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEHNAKTKPREEQYNSTYRVVSVLTVLHQD
WLNGKEYKCKVSNKALAPIEKTISKAKG(SEQ ID NO.6)的肽序列，或具有至少80％、至少85％、至少90％、至少95％、至少98％、或至少99％序列相似性的肽序列。在一些实施方案中，6D8抗体的CH3结构域是指包含
QPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF
LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK(SEQ ID NO.7)的肽序列。在一些实施方案中，6D8抗体的可变重链结构域是指包含GenBank登录号AEB96146的肽序列，或具有至少80％、至少85％、至少90％、至少95％、至少98％、或至少99％序列相似性的肽序列。在一些实施方案中，6D8抗体的可变轻链结构域是指包含GenBank登录号AEB96146的肽序列，或具有至少80％、至少85％、至少90％、至少95％、至少98％、或至少99％序列相似性的肽序列。在一些方面，编码轻链变体结构域的核酸在GenBank登录号HQ407546中列出。在一些方面，编码重链变体结构域的核酸在GenBank登录号AEB96148中列出。
如本文所用，术语“连接”当用于描述蛋白质结构或蛋白质结构域的构型时是指两个结构域或结构部分之间的连接，优选其中除了接头之外没有其他在中间的结构域或结构部分。在一些方面，如本文所用的术语“接头”是指蛋白质结构域或结构部分之间的氨基酸序列，其中接头的唯一功能是连接所述蛋白质结构域或结构部分。例如，接头是由甘氨酸、缬氨酸和/或苏氨酸残基组成的序列。在一些实施方案中，接头是由甘氨酸、缬氨酸、苏氨酸、丙氨酸和/或丝氨酸残基组成的序列。在一些方面，接头由长度为1至50个的氨基酸组成，例如由长度为3、4、6、10、12、14或16个的氨基酸组成。在某些实施方案中，接头是柔性接头。
如本文所用，术语“单元”是指作为更大的多组分蛋白质的功能片段的单个肽。单个肽可以是抗原性片段或蛋白质的结构域，例如免疫球蛋白的结构域。因此，如本文所用，对包含两个肽单元的重组蛋白的描述是指具有两个此类功能片段的重组蛋白(例如肽的二聚体)，这两个功能片段可以连接在一起或可以不连接在一起。
如本文所用，术语“免疫复合物”是指包含与其同源抗原结合的免疫球蛋白分子或其片段的复合物。如本文所用，术语“重组免疫复合物”或“RIC”是指不由自然产生免疫复合物中的免疫球蛋白分子的原始物种产生的免疫复合物。例如，示例性重组免疫复合物包含在植物中合成的人免疫球蛋白。
本公开涉及免疫球蛋白变体。在特别优选的实施方案中，本公开涉及作为免疫球蛋白G变体的重组蛋白，在本文中也称为IgG融合体。尽管与免疫球蛋白的天然结构有所不同，但所描述的重组蛋白确实具有免疫原性潜力。事实上，重组蛋白诱导的免疫应答与预期的重组蛋白中的抗原的免疫应答是同一类。本文所述的重组蛋白适用于产生针对多种病原体(例如寨卡病毒和诺如病毒)的疫苗。
尽管免疫球蛋白结构有变化，但Fc的糖基化状态强力地控制其功能。通过调节Fc的稳定性、构象和聚集，糖基化可以增强或抑制与Fcγ受体、FcRn和C1q的结合。这些改变导致抗体效应子功能的重要差异，所述抗体效应子功能包括抗体依赖性细胞毒性(ADCC)、抗体依赖性细胞介导吞噬作用(ADCP)、补体依赖性细胞毒性(CDC)和抗体依赖性病毒感染增强(ADE)。糖工程的进步已允许在各种重组表达系统中针对性地优化Fc糖基化状态。使用缺乏核心木糖和岩藻糖N-聚糖的糖工程化植物，产生了一种与FcγRI、FcγRIIIa和C1q的结合得到改善的抗CD20抗体。与哺乳动物细胞中产生的商业抗CD20抗体相比，该抗体具有增强的ADCC和CDC。类似地，已证明在糖工程化植物中产生的抗DENV抗体放弃了其ADE活性，因此与哺乳动物细胞产生的对应物相比，它们具有更好的功效和安全性数据。在糖工程化植物中制造的抗体治疗剂已用于治疗患有埃博拉病毒、HIV和基孔肯雅(Chikungunya)病毒病的恒河猴和人。
已鉴定了Fc区中赋予抗体期望的特性的突变。M252Y、S254T和T256E突变的引入通过改善低pH条件下与FcRn的相互作用，在人中将抗呼吸道合胞病毒抗体的血清半衰期从20天增加到60天。H237Y突变减少了铰链区的有害切割，同时提高了FcγRIII结合和ADCC活性。已报道了设计额外的二硫键以防止Fc融合体的去折叠和聚集。S239D和I332E突变改善了CD37抗体的FcγRIII结合和ADCC活性。用来自IgG3或骆驼抗体的较长铰链区替换IgG1铰链区增加了表皮生长因子受体抗体的ADCC。具有E345R、E430G和S440Y突变的IgG1形成了大大增强C1q结合和补体激活的六聚体。突变T437R和K248E也促进了OX40受体抗体的多聚化并改善了效应子功能。
可以使用人样(human-like)糖基化适当地组装本文所述的IgG变体，并在植物中以非常高的水平表达。这些构建体可以在植物中有效地制造，适当地组装，并通过蛋白G柱层析纯化。如实施例所示，纯化的重组蛋白具有很强的免疫原性。
如本文所述的实施例所示，优化抗体设计的各个方面(例如复合物大小)以产生所述免疫球蛋白变体，导致植物中优化的表达水平和改善的稳定性、溶解度和免疫受体结合。在将免疫球蛋白变体设计为以寨卡病毒包膜蛋白结构域III的一部分作为抗原的某些实施方案中，通过施用免疫球蛋白变体而产生的总IgG滴度是通过施用单独的抗原而产生的总IgG滴度的150倍。事实上，仅施用两剂免疫球蛋白变体而没有任何佐剂，终点滴度>1:500,000。可以以超过抗体治疗剂商业可行性的估计阈值的水平产生重组蛋白。
重组蛋白
本文所述的重组蛋白是变体免疫球蛋白结构，其包含抗原单元、Fc融合体单元、IgG的可变重链(VH)结构域单元和IgG的CH1结构域单元。Fc融合体包含IgG的CH2结构域和CH3结构域。与免疫球蛋白Fc结构域的蛋白质融合体是非常成功的治疗剂。它们可以提高融合伴侣的溶解度和稳定性，同时还提供一种简单且经济高效的纯化方法。此外，Fc融合体可以通过与体内的新生儿Fc受体(FcRn)相互作用来避免溶酶体降解，从而延长Fc融合体的血清半衰期。与单独的抗原相比，重组蛋白诱导针对附着在重组蛋白上的抗原的更强的免疫应答。因此，重组蛋白增加了抗原的免疫原性。
抗原单元不是IgG的表位。在某些实施方案中，抗原单元来自与IgG的表位不同的生物体。抗原单元的氨基酸序列长度介于5和500个残基之间。在某些实施方案中，抗原单元的长度介于5和400个残基之间。例如，抗原单元的长度介于5和300个残基之间、介于5和250个残基之间、介于5和200个残基之间、介于5和100个之间、介于9和300个残基之间、介于9和250个残基之间、介于9和200个残基之间、介于9和100个残基之间、介于10和300个残基之间、介于10和250个残基之间、介于10和200个残基之间、介于10和100个残基之间、介于20和300个残基之间、介于20和250个残基之间、介于20和200个残基之间、介于20和100个残基之间、介于30和300个残基之间、介于30和250个残基之间、介于30和200个残基之间、介于30和100个残基之间、介于50和300个残基之间、介于50和250个残基之间、介于50和200个残基之间、或介于50和100个残基之间。在一些方面，抗原单元包含串联连接的表位。
在一些方面，串联连接的表位包含不同的表位，其可能来自或可能不来自相同或相似的抗原蛋白。例如，靶向多种诺如病毒的重组蛋白可以包含来自多种诺如病毒的相同靶标抗原上的表位(见图15-图19中研究的重组免疫复合物)以解决诺如病毒多样性基因型的问题。因此，串联连接的表位可以包含来自相同抗原蛋白的表位。在其他方面，串联连接的表位是单个表位的重复。
抗原单元在N-末端与IgG的VH结构域单元连接或在C-末端与IgG的CH3结构域连接。IgG的CH1结构域单元与IgG的CH2结构域连接。在一些方面，抗原单元、Fc融合体单元、IgG的VH结构域单元、和IgG的CH1结构域单元形成重组蛋白的一半。例如，重组蛋白在产生时自组装，其中在CH2结构域和CH1结构域的连接处形成二硫键以连接两个Fc融合体单元(见图1A和图7)。因此在一些实施方案中，重组蛋白包含两个抗原单元、两个Fc融合体单元、两个IgG的VH结构域单元和两个IgG的CH1结构域单元。在某些实施方案中，抗体是6D8抗体。
在某些实施方案中，重组蛋白包含抗原单元、包含IgG的CH2结构域和CH3结构域的Fc融合体单元、IgG的VH结构域单元、IgG的CH1结构域单元；和IgG的轻链可变(VL)结构域单元。抗原单元不是IgG的表位，它在N-末端与IgG的VH结构域单元连接或在C-末端与IgG的CH3结构域连接。IgG的CH1结构域单元与IgG的CH2结构域连接，而IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域融合。
在一些方面，重组蛋白在产生时自组装，在IgG的CH2结构域和CH1结构域的连接处形成的二硫键连接两个Fc融合体单元。因而，重组蛋白包含两个抗原单元、两个Fc融合体单元、两个IgG的CH1结构域单元、两个IgG的VH结构域单元和两个IgG的VL结构域单元。在一些方面，每个IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域连接。在特定实施方案中，重组蛋白不包含IgG的任何轻链恒定(CL)结构域。此类实施方案的重组蛋白是单链抗体变体(见例如图7)。如图8所示，单链抗体变体可以在植物中产生并有效纯化。IgG为6D8抗体的单链抗体变体也保留了其结合6D8表位的能力(图11)。
在重组蛋白的一些实施方案中，重组蛋白包含两个抗原单元、两个Fc融合体单元、两个IgG的CH1结构域单元、两个IgG的VH结构域单元和两个IgG的VL结构域单元，重组蛋白进一步包含两个表位标签单元，其中表位标签是IgG的表位。在一些实施方案中，两个表位标签单元在IgG的CH3结构域的C-末端与两个Fc融合体单元连接，两个抗原单元与两个IgG的VH结构域单元连接。在其他实施方案中，两个表位标签单元与两个抗原单元连接，两个抗原单元在IgG的CH3结构域的C-末端处与两个Fc融合体单元连接。其中抗体是6D8抗体，表位标签包含肽序列YKLDIS(SEQ ID NO.1)。在一些方面，表位标签包含序列VYKLDISEA(SEQ IDNO.2)。在其他方面，表位标签由序列YKLDIS(SEQ ID NO.1)组成。
在其他实施方案中，重组蛋白包含抗原单元、包含IgG的CH2结构域和CH3结构域的Fc融合体单元、IgG的VH结构域单元、IgG的CH1结构域单元、IgG的VL结构域单元；和IgG的CL结构域单元。在此类实施方案中，IgG是6D8抗体，Fc融合体包含选自以下的至少一个置换突变：E345R、E430G、和S440Y。在某些实施方案中，Fc融合体包含置换突变E345R、E430G和S440Y。抗原单元不是6D8抗体的表位，它在C-末端与IgG的CH3结构域连接。IgG的VL结构域单元与IgG的CL结构域单元连接，IgG的CL结构域单元与IgG的CH1结构域单元连接。然后将IgG的CH1结构域单元与IgG的CH2结构域连接。在一些方面，重组蛋白进一步包含用于6D8抗体的表位标签，例如包含肽序列YKLDIS(SEQ ID NO.1)。在一些方面，重组蛋白中的表位标签包含序列VYKLDISEA(SEQ ID NO.2)。在其他方面，表位标签由序列YKLDIS(SEQ ID NO.1)组成。
在重组蛋白的另外其它实施方案中，IgG变体包含两个抗原单元、两个Fc融合体单元、两个IgG的VH结构域单元和两个IgG的CH1结构域单元。抗原不是IgG的表位，两个抗原单元在N-末端与两个IgG的VH结构域单元连接。两个IgG的CH1结构域单元与两个Fc融合体单元在IgG的CH2结构域处连接。在一些方面，重组蛋白不包含IgG的CL结构域并且不包含IgG的VL结构域。
寨卡病毒
寨卡病毒(ZIKV)是严重的全球健康威胁，缺乏安全、负担得起和有效的疫苗。除了其广泛的治疗价值外，IgG融合体由于其安全性和自佐剂(self-adjuvating)性质而是有前景的候选疫苗。
在示例性实施方案中，将一些IgG变体设计为生成针对ZIKV的免疫应答。因此，重组蛋白中的抗原是靶向ZIKV的抗原。在一些方面，抗原靶向寨卡病毒包膜结构域III(ZE3)。这些IgG变体在植物中高度稳定且高度表达。因此，本文所述的重组蛋白是候选疫苗，例如针对ZIKV。
在一些方面，靶向ZE3的抗原包含登录号AMC13911.1的K301-T406。在其他方面，靶向ZE3的抗原包含与登录号AMC13911.1的K301-T406具有至少80％、至少85％、至少90％、至少95％、至少97％、至少98％或至少99％序列相似性的肽序列。在另外其他方面，靶向ZE3的抗原包含这样的序列，其是来自其他寨卡病毒毒株的GenBank登录号AMC13911的相应区域的功能等效形式，例如在以下GenBank登录号中的ZE3的相应序列：AY632535、KU321639、KJ776791、KF383115、KF383116、KF383117、KF383118、KF383119、KF268948、KF268949、KF268950、EU545988、KF993678、JN860885、HQ234499、KU501215、KU501216、KU501217。
本文描述的靶向ZIKV的重组蛋白在没有佐剂且仅两剂的情况下引发针对ZIKV的强烈免疫应答(图6A、图6B)。许多重组蛋白在植物中以高水平产生(0.5-1.5mg/g LFW)(图2)，并且在反复冻融和储存后稳定(图5)。与单独的ZE3抗原相比，最好的IgG融合体组产生高出100倍以上的抗体应答。这与先前的研究一致，所述先前研究表明ZE3或ZIKV E本身具有弱免疫原性，需要佐剂和3-4剂。
预期形成聚合物结构的构建体对降解的抗性最强，并且具有最高的总产率。通过优化RIC的分子间结合，生成了更小的复合物，其溶解度大大提高，产生1.5毫克IgG融合体每克叶鲜重(mg/g LFW)，并具有等效或改善的免疫原性特性。当用于免疫小鼠时，使用无佐剂仅两剂IgG融合体即可引发高滴度的寨卡病毒特异性抗体，超过单独的ZE3产生的滴度20倍至150倍，并有效中和寨卡病毒。与未融合的ZE3抗原相比，还发现IgG融合体以与C1q结合相关的方式强烈增强IgG2a的产生。这些发现证明了IgG融合体作为可以在植物中有效制备的寨卡病毒自佐剂亚单位疫苗的优异潜力。
ZE3与IgG N-末端的融合增强了靶向ZIKV的IgG融合体的C1q结合。去除IgG轻链(HVL和HVL相关构建体)或Fab区也增强了C1q结合。在IgG Fc区添加诱导六聚体的突变也增强了C1q结合。也通过添加自结合表位标签增强了C1q结合，以创建RIC或在缺乏植物特异性β1,2-连接木糖和α1,3-连接岩藻糖N-聚糖的植物中产生IgG融合体(例如，通过沉默木糖基转移酶和岩藻糖基转移酶，见图9和图10)。与未复合的IgG相比，RIC构建体(HLZe)和基于单链抗体变体的修饰免疫复合物(HVLZe)均显著改善了C1q结合(图1、图9和图10)。免疫后的结果表明，HVLZe构建体产生的抗体滴度与包含完整重链和轻链的传统RIC产生的抗体滴度相似(图6A和图6B)。
在一些方面，RIC构建体不是优选的，因为大的复合物尺寸使得在提取时其溶解性差(图2A和图10)，并且高浓度可能在储存过程中沉淀。非常大的RIC也可能太大而无法从注射部位有效地引流到淋巴结，颗粒<200nm有利于该过程。已发现六聚体大小的IgG是有效结合C1q的最佳底物。使6D8表位标签突变(构建体HLZd)也可以缓解RIC构建体的问题。值得注意地，与RIC相比，HLZd的可溶性显著更高(图13B)，具有中等密度(图4A)，显示出更高的C1q结合(图1B)，达到更高的表达水平(1.5mg/g LFW)(图2A)，并引发更高的IgG2a滴度(图6A和图6B)。这些对RIC候选疫苗设计的改进适用于其他抗原。
HVLZe融合体具有比传统RIC稍高的C1q结合(图1B)，并且在测试条件下具有高稳定性(图5A)。由于与完整6D8抗体相比，这种单链构型减少了与表位标签的结合，因此HVLZe形成更小的复合物(图4A中，与HLZe相比，蔗糖梯度数据显示密度降低)。然而，一些非常致密的物质仍然与构建体HLZd不同。可以通过减少HVLZe的表位结合来提高溶解度。
表1总结了重组蛋白(靶向ZIKV的抗原)的特征。对于表达，仅显示了完全组装产物的产量(mg/g LFW)。更多数量的“+”符号表示该特性的平均值在统计学上显著增加(对于C1q、IgG和IgG2a)，或可重复观察到差异(对于密度)。对于ZHx(*)，观察到低密度和高密度物质的峰。
表1.
构建体 表达 溶解度 密度 稳定性 C1q IgG IgG2a
ZE3 N.D. N.D. N.D. N.D. N.D. + +
HLZ 0.17mg/g 高 + 中 + +++ ++
HLZe 0.22mg/g 低 ++++ 高 ++ +++ ++
HLZd 1.50mg/g 高 ++ 高 +++ +++ +++
ZH 0.83mg/g 高 + 中 ++ +++ +++
ZHx 0.21mg/g 低 +++* 高 +++ +++ +++
VHLZe <0.1mg/g 低 ++++ 高 +++ +++ +++
ZFc 0.57mg/g 高 + 低 +++ +++ +++
诺如病毒
人诺如病毒(HNoV)感染的广泛发生造成了巨大的经济损失和疾病负担，然而没有可用的获得批准的疫苗或特定的治疗方法。HNoV的巨大遗传多样性阻碍了控制这些病毒的努力，这些病毒不断进化以逃避天然免疫。大量已发表的抗体图谱和T细胞数据表明存在广泛保守的HNoV B细胞和T细胞表位，至关重要地，人免疫系统可靶向这些表位。因此，将有可能设计重组蛋白，用于引发针对广泛保守的HNoV抗原靶标的免疫应答的目的。
在示例性实施方案中，设计IgG变体以生成针对诺如病毒(NoV)的免疫应答。在一些方面，重组蛋白的抗原包含来自多种诺如病毒的表位，例如来自人诺如病毒GI.3、人诺如病毒GII.4、和/或鼠诺如病毒(MNV)。靶向诺如病毒的重组蛋白中的抗原包含至少一个来自NoV的主要衣壳蛋白(VP1)或NoV的非结构蛋白的表位。在一些方面，抗原靶向VP1的突出结构域或壳结构域。此类重组蛋白是针对NoV的候选疫苗。
在某些实施方案中，可以组合使用靶向NoV的重组蛋白，用作疫苗组合物。如实施例和图16-图19所示，这些抗原已成功用于生产能够广泛结合、阻断和中和来自不同基因组和基因型的一组不同NoV的免疫球蛋白变体。
在一些方面，靶向VP1的抗原包含VP1和/或NS1的5至500个残基长的部分。在一些实施方案中，抗原单元包含在SEQ ID NO.34-72中列出的至少一个序列、至少三个序列、至少六个序列、至少九个序列、至少十二个序列、或至少十五个序列。例如，靶向VPl的重组蛋白中的抗原单元包含在SEQ ID NO.34-54中列出的至少一个序列、至少两个序列、至少三个序列、至少四个序列、至少五个序列、至少六个序列、至少七个序列、至少八个序列、或至少九个序列。在具体的实施方案中，靶向VPl的突出结构域的抗原单元包含选自SEQ IDNO.34-44的至少一个序列、至少两个序列、至少三个序列、至少四个序列、至少五个序列、至少六个序列、至少七个序列、至少八个序列、或至少九个序列。在某些实施方案中，靶向诺如病毒的重组蛋白的抗原单元包含SEQ ID NO.34-42中列出的序列。在另一个具体的实施方案中，靶向VP1壳结构域的重组蛋白的抗原单元包含选自SEQ ID NO.45-54的至少一个序列、至少两个序列、至少三个序列、至少四个序列、至少五个序列、至少六个序列、至少七个序列、至少八个序列、或至少九个序列。在一些方面，靶向诺如病毒的重组蛋白的抗原单元包含SEQ ID NO.45-53中列出的序列。在又一个具体的实施方案中，靶向NS1的重组蛋白的抗原单元包含选自SEQ ID NO.55-57的至少一个序列或至少两个序列。在一些方面，靶向诺如病毒的重组蛋白的抗原单元包含SEQ ID NO.55-57中列出的序列。在另一个具体的实施方案中，设计用于生成T细胞介导的免疫应答的重组蛋白的抗原单元包含选自SEQ IDNO.58-72的至少一个序列、至少两个序列、至少三个序列、至少四个序列、至少五个序列、至少六个序列、至少七个序列、至少八个序列、或至少九个序列，例如，抗原单元的肽序列包含SEQ ID NO.58-72的序列。
生产方法
与哺乳动物细胞系统相比，植物重组表达系统具有固有的安全性、高可扩展性和低生产成本，使其特别适合制造IgG融合体疫苗。虽然先前的工作已表明一些IgG融合体疫苗可以增强抗原免疫原性，但尚未直接比较已开发的许多融合策略，使得难以确定创建最佳候选疫苗所涉及的关键特性。
本文所述的生产方法包括在转基因植物中表达重组蛋白。在某些实施方案中，重组蛋白在木糖基转移酶和岩藻糖基转移酶被沉默的转基因植物中表达。在一些方面，例如重组蛋白是RIC，优选使用木糖基转移酶和岩藻糖基转移酶被沉默的植物来产生蛋白质。所述方法包括将双元载体引入农杆菌，所述双元载体表达抗原单元、Fc融合体单元、IgG的VH结构域单元；和IgG的CH1结构域单元。因此，该双元载体编码IgG的重链结构域。接着，用含有双元载体的农杆菌渗入植物部分以产生转化的植物部分。提取植物部分的粗蛋白，然后纯化得到重组蛋白。在一些方面，使用本领域公认的方法(例如蛋白G亲和层析或金属亲和层析)纯化重组蛋白。
当重组蛋白包含IgG的VL结构域单元和IgG的CL结构域单元时，该方法进一步包括将不同的双元载体引入农杆菌中，所述不同的双元载体编码IgG的轻链结构域，即IgG的VL结构域单元和IgG的CL结构域单元。植物部分的转化包括用含有编码IgG重链结构域的双元载体的农杆菌和含有编码IgG轻链结构域的双元载体的农杆菌共渗入植物部分。
在具体的实施方案中，生产本文所述的重组蛋白的方法包括将选自以下的载体引入农杆菌：pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、pBYKEMd-HZE3、pBYKEMd-ZE3H、pBYKEMd-ZE3Hx、pBYKEMd-HVLZe、pBYKEMd2-HVL-Hx、pBYKEMd2-HVLZnt、pBYKEMd2-ZHVLnt、pBYKEMd2-ZHVLe、pBYKEMd2-ZHVLhx、pBYKEAM-N12MHd、pBYKEAM-NPHd、pBYKEAM-NSHd、pBYKEAM-NTHd、pBYKEHM-CPHd、pBYKEHM-CSHd、和pBYKEHM-CTHd，并用含有所述载体的农杆菌渗入植物部分以产生转化的植物部分。然后从转化的植物中提取粗蛋白，然后纯化重组蛋白。对于其中引入农杆菌的载体选自pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、和pBYKEMd-HZE3的生产方法，所述方法进一步包括用含有pBYKEMd-6D8K的农杆菌与含有所述载体的农杆菌共渗入。
根据某些实施方案的说明性、非限制性实施例
通过以下实施例进一步说明本公开，所述实施例不应被解释为限制性的。本申请全文中引用的所有参考文献、专利和公开的专利申请的内容以及附图，出于所有目的通过引用整体并入本文。
A.具有ZIKV包膜结构域III的IgG融合体的设计和C1q结合
ZE3是有前景的候选亚单位疫苗，但它本身没有强烈的免疫原性，使得需要有佐剂的高抗原剂量和重复免疫。基于与ZE3融合的人源化埃博拉病毒单克隆抗体6D8设计了一组人IgG1变体(图1A)。通过柔性接头将ZE3与6D8重链的C-末端融合，创建了RIC构建体。用6D8表位结合位点标签化ZE3以允许形成免疫复合物。该构建体称为“HLZe”，因为它含有重链、轻链、C-末端ZE3融合体和表位标签。作为免疫复合物形成的对照，创建了一个没有表位标签、其他均相同的构建体(构建体“HLZ”)，因此预期不会形成免疫复合物。首先，研究了IgG变体的C1q结合。为了改善免疫受体结合，在糖工程化的本氏烟草中表达所有构建体。与在野生型植物中表达的构建体相比，在糖工程化植物中制造的6D8抗体或RIC显示出高度改善的C1q结合(图9)。由于密集簇集的抗原-抗体复合物而认为RIC以高亲合力与C1q结合，并且正如预期的那样，与HLZ相比，HLZe显示出大大改善的C1q结合(p<0.001)(图1B)。与HL相比，也注意到HLZ的C1q结合小幅增加，这可能表明构建体的低水平聚集(p<0.05)(图1B)。
据报道，除非同源抗原与抗体结合，否则抗体Fab臂可以通过抑制C1q结合在补体激活中发挥调节作用。与这些数据一致，加入携带6D8表位标签的可溶性抗原改善了C1q结合(图10，比较HL与HL+Ag)，并且通过去除6D8轻链或有趣地通过抗原与6D8重链N-末端融合，大大增强了C1q结合(图10，比较HL、H和ZHL)。因此，在没有轻链的情况下，创建了ZE3与6D8的N-末端融合的构建体(构建体“ZH”)，并发现其有效结合C1q(图1B)。为了进一步改善与C1q的相互作用，创建了除了将E345R、E430G和S440Y突变引入6D8 Fc区之外与ZH相同的构建体，其有利于形成六聚体。与ZH相比，该构建体(ZHx)进一步改善了C1q结合(p<0.001)(图1B)。有趣地，虽然已显示IgG1-Fc融合体具有可变的C1q结合，但与6D8 Fc融合的ZE3(构建体“ZFc”)显示出非常强烈的C1q结合(图1B)。为了构建也缺乏轻链恒定区的简化RIC，首先制造构建体，使得6D8的可变轻链(VL)结构域插入6D8的可变重链(VH)结构域和CH1结构域之间，产生构建体HVL。发现该构建体与6D8表位标签化的抗原结合，尽管与未改变的6D8相比水平有所降低(图11)。将该构型插入HLZe以创建单链RIC HVLZe，其显示出有效C1q结合(图1B)。
注意到有趣的是，理论上为单体的构建体HLZ(由与6D8的C-末端融合的ZE3组成，没有任何表位标签)在C1q结合方面产生了小的但可重复的改善(图1B)。这可以表明由于ZE3融合导致一些低水平的聚集(图4A，比较HL和HLZ)。此外，据报道，Fab介导的事件影响C1q结合，因为IgG Fab臂的缺失诱导形成寡聚体并通过未知机制进一步增强C1q结合。HLZ通常低表达，表明C-末端ZE3融合体可能干扰折叠或以其他方式导致不稳定(图2A)。在纯化后通过SDS-PAGE可见许多降解产物(图3)，有趣的是，通过在RT孵育2周或重复冻融循环进一步降解时，HLZ的C1q结合继续增加(图5B)。这些数据表明降解产物可能比原始构建体具有更高的免疫原性，这一发现可以解释HLZ产生的相对较高的总IgG滴度(图6A)。然而，与其他构建体相比，HLZ IgG2a滴度降低(图6B)。由于IgG2a是Th1应答的一般指标，HLZ可能仍具有导致T细胞活化的受损效应子功能，如减少的补体激活。值得注意地，与未融合的ZE3相比，所有IgG融合体强烈地增强IgG2a的产生(图6B)。虽然最近显示与小鼠抗体相比，人抗体对小鼠Fc受体具有相似的结合亲和力，但人IgG1与低亲和力小鼠FcγRIII/CD16的结合降低。还显示与同一IgG融合体的单体形式相比，聚合登革热IgG融合体疫苗在人腺体扁桃体组织中显示出增强的免疫原性，然而两者在表达人FcγRI/CD64的小鼠中显示出等同的免疫原性。因此，需要在其他动物模型中进行进一步测试，以更全面地评估这些构建体的免疫原性。
抗原结合可以引起IgG的构象变化，这可能改善C1q结合。将6D8与含有6D8表位的抗原混合仅产生小幅增加的C1q结合(图10)，并且与RIC构建体相比，发现其免疫原性较差(数据将在别处显示)。然而，N-末端抗原融合体对C1q结合具有更显著的影响(图1B)，可能是通过强烈诱导类似于抗原结合的构象变化。一般地，与6D8 N-末端的抗原融合体大大增强对各种大和小抗原的C1q结合。此外，6D8轻链的缺失(构建体ZH)也显著增强了C1q结合(图1B)。这些发现与抗体的Fab部分在C1q结合中起调节作用的假设一致。此外，去除轻链也可能影响糖基化，从而影响C1q结合。构建体ZHx将去除轻链和N-末端ZE3融合体的效果与诱导六聚体的突变协同组合，导致更有效地增强C1q结合(图1B)和高的总IgG和IgG2a滴度(图6B)。尽管ZHx具有强烈的免疫原性，但与ZH相比，ZHx的产量降低(图2A)。此外，ZHx表现出在蔗糖梯度的最底部发现了一些在构建体ZH中未发现的非常大的物质(图4B)，表明六聚体突变可能有助于聚集。需要进一步的工作来优化这些构建体的溶解度。
N-末端ZE3与6D8 CH1结构域的融合体(构建体ZFc)大大改善了C1q结合(图1B)，引发了非常高的抗体滴度(图6A)。Fc融合体的许多特性(包括C1q结合)看来基于个体融合伴侣而不同，因此必须对每个融合体根据经验确定。有趣地，已显示一些Fc融合构建体形成六聚体，这可以解释此处观察到的ZFc的强烈免疫原性。尽管ZFc具有强烈的免疫原性，但ZFc非常不稳定：即使在SDS样品缓冲液中直接提取时，也有50％的ZE3在提取之前或提取过程中被切割下来(图2A、图2B)，并且在反复冻融或储存后，少于25％的全尺寸ZFc分子保持完整(图5A)。与HLZ不同，这种降解与C1q结合的丧失有关(图5B)，表明Fc受体结合结构域受损。ZFc的二硫键少于整个IgG，一般地，Fc融合体经常遭受不稳定性或不期望的聚集的问题。虽然ZFc引发了高滴度，但要递送8μg ZE3(扣除IgG融合伴侣的重量)，用于免疫的纯化样品的总量必须几乎是三倍，以对ZE3融合体的高度降解和导致的损失负责。尚不清楚未融合的Fc以及其他可能的降解产物的这种增加的递送是否导致了高免疫原性。相比之下，更大且更高度寡聚的构建体HLZe、HLZd、ZHx和HVLZe(图4A和4B)在提取之前和提取过程中以及储存后(图5A)都更稳定。与其在ELISA中不可与抗体探针接触一致(图2A)，这些构建体的分子内和分子间结合可以保护它们免受蛋白水解或其他降解过程的影响。
B.HVL融合构建体的表达和表征
已知RIC具有免疫原性；然而，它们需要共表达重链和轻链以形成完全组装的产物。可以通过创建单链抗体融合体来简化该过程。单链抗体融合体的益处是可以在单个编码序列中产生整个抗体-抗原融合体，从而消除了共表达重链和轻链的需要。此外，新构建体仍将保留重链和轻链的可变区。核心单链抗体含有与可变轻链区连接的可变重链区，所述可变轻链区直接与抗体重链融合(图7)。虽然先前已表征和发表了在本氏烟草植物中表达的类似构建体，但很少有工作探索其中核心单链抗体与感兴趣的抗原融合的单链抗体融合体的潜力。创建并测试了数个单链抗体融合体(图7)。纯化构建体的结果可以在植物中表达，并在非还原条件下在SDS-PAGE凝胶上跑胶时显示为正确的大小。
C.IgG融合构建体的表达
RIC遭受可溶性产物产率低的问题。在6D8的C-末端添加6D8表位标签使得抗体几乎不溶；然而可以通过去除轻链来防止这种情况，这表明不溶性是由与表位标签结合的大抗体复合物引起的(图12)。为了提高RIC溶解度，突变了6D8表位标签以减少抗体结合。将HLZe上的表位标签减少到6D8的最小报道结合区(Wilson等人，2000)(构建体“HLa”，表位序列VYKLDISEA，SEQ ID NO.2)显示溶解度没有改善，从3'端去除单个氨基酸(构建体“HLb”，VYKLDISE，SEQ ID NO.3)也显示没有改善(图13A)。然而，从5'端进一步去除单个氨基酸(构建体“HLc”，YKLDISE，SEQ ID NO.4)和构建体“HLd”中额外的截断(YKLDIS，SEQ ID NO.1)导致溶解度大大提高(图13B)。将该突变引入HLZe(构建体HLZd)并进行表征。尽管通过ELISA测定表位结合降低了大约25倍(图13C)，但HLZd仍然保持非常强烈的C1q结合(图1B)，表明保持了有效的复合物形成活性。
为了测量完全组装的IgG融合体的产量，采用了ELISA测定，首先捕获ZE3，然后检测人IgG。为了检测ZE3的切割，还使用仅测量总IgG的ELISA作为比较。当探测ZE3和IgG时，高度可溶的单体构建体ZH产生每克叶鲜重0.83mg完全形成的产物(mg/g LFW)，类似的ZFc产生0.58mg/g LFW(图2A)。然而当测量总IgG含量时，ZH的产量大约高20％，ZFc则高2.5倍，这表明ZH尤其是ZFc易受蛋白水解切割，可能是切割ZE3接头(图2A)。通过SDS-PAGE可视化ZFc切割产物而证实了这一发现(图2B)。HLZ仅累积0.17mg/g LFW完全组装产物，大约40％的ZE3因切割而丢失，表明构建体的总体不稳定性(图2A、图2B)。通过ELISA测定，RIC构建体的性能看来比单体构建体差：HLZe和HLZd分别仅累积0.04mg/g LFW和0.30mg/g LFW，六聚体ZHx仅累积0.08mg/g LFW(图2A)。然而，当在还原SDS-PAGE条件下可视化时，HLZd是最高表达的构建体，累积估计为1.5mg/g LFW(图2A、图2B)。这种差异可能是由于复合HLZd无法通过ELISA与抗体探针接触。类似地，当通过凝胶定量测量时，HLZe和ZHx的总产率也更高(图2A、图2B)。尽管其溶解度低，但HLZe的产率与HLZ相似，HLZd大大超过HLZ的产率。这一发现表明，形成复合物可以在细胞内或在提取过程中保护HLZe和HLZd，尽管不能排除短的表位标签在增强HLZe和HLZd稳定性方面发挥一些其他作用的可能性。通过ELISA测定HVLZe仅产生0.02mg/g LFW可溶产物，而通过SDS-PAGE无法检测到(图2A)；然而当用7.5M尿素提取时，它积累了非常高的水平，这表明不溶性导致了低产率。
D.IgG融合体的纯化、聚集和稳定性
通过蛋白G亲和层析使用简单的一步纯化，将IgG融合体纯化至>95％的同质性。与表达数据一致，更高度寡聚的构建体显示出比其他构建体更少的降解，而ZFc融合体具有特别高的降解水平(图3)。为了研究每个构建体的聚集特征，通过蔗糖梯度离心分析了纯化的IgG融合体。与形成大的免疫复合物一致，发现HLZe和HVLZe主要位于梯度的底部，而HLZ、ZH和ZFc主要位于梯度的顶部(图4A和图4B)。ZHx溶液含有低密度物质以及一些非常高密度的物质(图4B)。与HLZ和HLZe相比，HLZd溶液显示出中等密度(图4A)，当结合表达数据和C1q结合时，该结果与HLZd比HLZe形成更小、更可溶的免疫复合物相一致。
通过在不同温度条件下处理后在SDS-PAGE上比较完全形成的产物和降解产物，分析了每个构建体的稳定性。在五个冻融循环或在4℃两周后，观察到所有构建体均有少量降解(图5A)。在4℃数天后，发现高浓度(>1mg/ml)的HLZe和HVLZe发生沉淀。在室温，大多数构建体在两周后降解了10-15％(图5A)。总体上，寡聚构建体保持最高稳定性，而单体构建体尤其是Fc融合体降解更快(图5A)。大多数构建体在储存后保持强烈的C1q结合，然而在室温储存或重复冻融循环而不是4℃储存后，构建体HLZ显示出增加的C1q结合(图5B)。这可能是由于Fab区的降解或轻链的丢失，因为降解产物在SDS-PAGE上可见(图3)，或是由于聚集。相反地，构建体ZFc随着其降解程度升高而失去了C1q结合能力，这可能是由于C1q结合区的降解(图5B)。
E.小鼠免疫、滴度、IgG2a、细胞因子
为了研究IgG融合体的免疫原性，用两剂每种IgG变体无佐剂皮下免疫BALB/c小鼠(n＝6)，使得递送的ZE3总剂量为8μg。作为对照，还用8μg未融合的植物表达的ZE3免疫小鼠。所有IgG融合体都非常强烈地增强了ZE3特异性抗体的产生，产生的总IgG滴度比单独的ZE3高20倍至150倍(图6A，与ZE3相比p<0.01)。与单独的ZE3相比，所有IgG融合体都显著增强了IgG2a的产生(图6B，与ZE3相比p<0.01)。虽然在IgG融合体之间未观察到总滴度的产生的显著差异，但与除HLZe之外的所有其他融合体相比，构建体HLZ的IgG2a的产生显著降低(图6B，与HLZ相比p<0.05)。构建体ZHx和ZFc产生最高水平的总IgG和IgG2a(图6A、图6B)。
F.诺如病毒表位的IgG融合体的设计、表达和纯化
诺如病毒(NoV)衣壳蛋白VP1由内壳结构域(S)和突出结构域(P)组成，所述内壳结构域形成围绕病毒基因组的核心，所述突出结构域进一步细分为P1和P2子结构域。传统的人诺如病毒(HNoV)疫苗方法以及天然感染引起的免疫反应主要靶向病毒衣壳上保守性差的免疫显性表位，导致针对新型毒株和针对不同基因型的相关病毒的保护有限。几乎所有HNoV候选疫苗都专注于由VP1衣壳蛋白制造的病毒样颗粒(VLP)。基于主要衣壳蛋白VP1的序列差异，已鉴定出十个遗传组(genogroups,GI-GX)，其含有超过四十种NoV基因型。在这十个遗传组中，GI和GII引起人的疾病最多。然而，即使是生成保守VLP的最佳尝试也仅在人临床试验中适度降低了疾病严重程度，并且通常难以诱导广泛的保护性免疫。
一种广泛反应性抗体定位(map)到在以下P1碱基处的高度保守表位：LPQEWVQYFYQEAAPA(SEQ ID NO.34)。至关重要地，该抗体结合P1中的线性表位，因此天然VP1构象对于引发功能性抗体不是必需的。靶向该表位的抗体以高亲和力结合来自8个GI、13个GII和1个GIV基因型的VLP。针对第二广泛反应性线性表位(ALLRFVNPDTGRVLFE,SEQ IDNO.37)的抗体结合来自至少3个GI和7个GII遗传组的VLP。在P1结构域碱基处的第三广泛保守的线性表位是DSWVNQFYTLAP(SEQ ID NO.41)。S结构域是VP1中最高度保守的。已鉴定了靶向强烈保守的线性表位QNVIDPWIRNNF(SEQ ID NO.45)、QAPGGEFTVSPRNAPGE(SEQ IDNO.46)和KVIFAAVPP(SEQ ID NO.47)的抗体对NoV遗传组具有广泛的交叉反应性。在人13中鉴定了保守的HNoV T细胞表位，即VP1 S结构域中的表位TMFPHIIVDV(SEQ ID NO.54)引发了针对GI和GII HNoV的广泛T细胞激活。此外，在所有NoV遗传组(包括HNoV)之间高度保守的P1 C-末端结构域中的显性T细胞表位(SWVSRFYQL,SEQ ID NO.64)对于感染NoV的小鼠的病毒清除至关重要。该区在衣壳组装中起着至关重要的作用，因此结构限制限制了突变逃逸的可能性。从NS1-2蛋白水解切割的非结构蛋白NS1由鼠NoV(GV)感染或用HuNoV NS1-2转染的细胞分泌。分泌的NS1抑制小鼠肠道中IFN-λ的产生。重要地，单独的NS1疫苗接种比P结构域疫苗接种更好地保护小鼠，突出显示了NS1作为疫苗靶标。NS1-2疫苗接种产生针对NS1的抗体。
为了开发抗原以制造靶向诺如病毒的免疫球蛋白变体，已鉴定了来自人诺如病毒GI.3、人诺如病毒GII.4和鼠诺如病毒(MNV)VP1突出结构域的表位(SEQ ID NO.34-44)。还鉴定了来自GI.3、GII.4和MNV的VP1壳结构域的表位(SEQ ID NO.45-54)。MNV的NS1表位在SEQ ID NO.55中列出。GI.3的NS1表位在SEQ ID NO.56中列出。GII.4的NS1表位在SEQ IDNO.57中列出。T细胞应答表位在SEQ ID NO.58-72中列出。
因此，设计和生产了RIC构建体，其靶向NoV突出结构域(P-RIC)、壳结构域(S-RIC)、T细胞表位(T-RIC)的保守表位，或含有串联连接的来自鼠诺如病毒的非结构蛋白1(NS1)(N-RIC)。P-RIC的抗原单元由九个串联连接的来自GI.3、GII.4和MNV的VP1突出结构域的表位形成。P-RIC的抗原单元由九个串联连接的GI.3、GII.4和MNV的VP1突出结构域的表位形成。S-RIC的抗原单元由九个串联连接的GI.3、GII.4和MNV的VP1壳结构域的表位形成。T-RIC的抗原单元由十五个串联连接的表位形成，已发现这些表位引起针对GI.3、GII.4和MNV的T细胞介导的免疫应答。N-RIC的抗原单元由串联连接的来自GI.3、GII.4和MNV的NS1的表位形成。用于产生这些RIC构建体的植物表达载体是pBYKEAM-N12MHd、pBYKEAM-NPHd、pBYKEAM-NSHd、pBYKEAM-NTHd、pBYKEHM-CPHd、pBYKEHM-CSHd、和pBYKEHM-CTHd。
对于每个表位，可以在植物中成功表达在N-末端或C-末端连接表位的RIC构建体(图14A)。可以纯化所有这些RIC构建体(图14B和图15)。来自用这些RIC构建体免疫的小鼠的血清可以结合小鼠诺如病毒(图16)和模拟GI.3和GII.2诺如病毒的VLP(图17和图18)。用RIC免疫的小鼠的脾细胞IFN-γ的产生增加(图19)，表明RIC成功诱导了免疫诺如病毒。
G.方法
1.载体构建
Diamos等人，2019中描述了ZE3的BeYDV植物表达载体的构建，以及其与6D8 C-末端的融合体(pBYR11eM-h6D8ZE3，此处称为构建体“HLZe”)或与N-末端的融合体并具有表位标签(pBYR11eMa-BAZE3-Hgp371)或没有表位标签(pBYR11eMa-BAZE3-H)。Diamos等人，2019中还描述了载体pBYKEMd2-6D8，其表达未融合ZE3的完整6D8 mAb(构建体“HL”)。为了创建仅表达6D8轻链的载体，用XhoI消化pBYKEMd2-6D8，载体自连接产生pBYKEMd-6D8K。通过用SacI消化pBYKEMd2-6D8并使载体自连接来创建仅表达6D8重链的载体(构建体“H”)，以产生pBYKEMd-6D8H。通过用BsaI-SacI消化pBYR11eMa-BAZE3-Hgp371并将含有标签的片段插入用BsaI-SacI消化的pBYKEMd-6D8H中，将6D8表位结合标签添加到pBYKEMd-6D8H，产生pBYKEMd-6D8Hgp371(当与轻链共表达时为构建体“HLe”)。为了从HLZe中去除表位标签，用BamHI-SacI消化pBYR11eM-h6D8ZE3并与含有ZE3的片段连接，所述含有ZE3的片段通过用引物ZE3-Bam-F(5’-gcgggatccaagggcgtgtcatactcc,SEQ ID NO.8)和ZE3-Sac-R(5’-acagagctcttaagtgctaccactcctgtg,SEQ ID NO.9)扩增并随后用BamHI-SacI消化获得。将所得载体pBYKEMd-HZE3与pBYKEMd-6D8K共渗入以产生构建体“HLZ”。为了产生与没有轻链的6D8 N-末端融合的ZE3，用SacI消化pBYR11eMa-BAZE3-H并使载体自连接，产生pBYKEMd-ZE3H(构建体“ZH”)。为了引入六聚体突变，合成了包含E345R、E430G和S440Y突变的6D8重链恒定区的区域(Integrated DNA Technologies,Iowa,USA)，然后用BsaI-SacI消化并用于替换pBYKEMd-ZE3H中6D8的BsaI-SacI区，产生pBYKEMd-ZE3Hx(构建体“ZHx”)。通过退火寡核苷酸6D89-F(5'-ctagtgtttacaagctggacatatctgaggcataagagct,SEQ ID NO.10)和6D89-R(5’-cttatgcctcagatatgtccagcttgtaaaca,SEQ ID NO.11)并将其连接到用SpeI-SacI消化的pBYR11eM-h6D8ZE3中，来生成RIC表位标签突变体“a”；通过首先用引物gpDISE-Sac-R：(5'-tttgagctcttactcagatatgtccagcttgtaaac,SEQ ID NO.12)和35S-F(5’aatcccactatccttcgc,SEQ ID NO.13)扩增突变体“a”，然后用SpeI-SacI消化产物并将其连接到用SpeI-SacI消化的pBYR11eM-h6D8ZE3中，来生成突变体“b”。与突变体“a”类似地创建突变体“c”和“d”，使用重叠寡核苷酸6D87-F(5'-ctagttacaagctggacatatctgagtaagagct,SEQ ID NO.14)和6D87-R(5’-cttactcagatatgtccagcttgtaa,SEQ ID NO.15)用于“c”，和6D86-F(5’-ctagttacaagctggacatatcttaagagct,SEQ ID NO.16)和6D86-R(5’-cttaagatatgtccagcttgtaa,SEQ ID NO.17)。
为了制造其中可变重链(VH)结构域与可变轻链(VL)结构域连接、所述可变轻链结构域又直接与6D8抗体的恒定区融合的构建体，首先通过pBYR11eM-h6D8ZE3片段的PCR扩增和末端剪裁(end-tailoring)获得可变区。对于VH结构域，使用引物LIR-H3A(5’-aagcttgttgttgtgactccgag,SEQ ID NO.18)和6D8VH-Spe-R(5’-cggactagtagctgaagacactgtgac,SEQ ID NO.19)。通过使用引物35S-F(5’-aatcccactatccttcgc,SEQ ID NO.13)和6D8VK-Nhe-R(5’-cgtgctagccttgatctccactttggtc,SEQ ID NO.20)PCR扩增pBYR11eM-h6D8ZE3来获得VL区。为了将VL区与人IgG抗体的恒定区融合，通过用XhoI-NheI消化PCR片段并将其插入含有6D8重链的载体pKS-HH-gp371中，创建了亚克隆。该亚克隆命名为pKS-VL。接着，用SbfI-SacI消化pBYKEM-6D8K，用SbfI-SpeI消化扩增了可变重链片段的PCR产物，并用SpeI-SacI消化可变轻链亚克隆。组装这些片段以创建pBYKEMd2-VHLVK(HVL)。最后，该构建体用于通过两片段连接来创建pBYKEMd2-HVLZe。用BsaI和SacI消化pBYKEMd2-VHLVK构建体以获得载体片段以及重链和轻链的可变区。为了获得ZE3抗原片段和表位标签，也用BsaI-SacI消化pBYR11eM-h6D8ZE3。所得构建体命名为pBYKEMd-HVLZe，用于生产HVLZe。
构建体的HVL小组如下创建。通过两片段连接创建HVL-Hx，其是含有单链抗体的构建体，所述单链抗体在Fc区中具有三个点突变以促进形成单链六聚体。主链源自pBYKEMd2-VHLVK的BsaI-SacI消化片段，而含有用于六聚体形成的突变的插入片段源自pBYKEMd-ZE3Hx的BsaI-SacI消化片段。最终构建体命名为pBYKEMd2-HVL-Hx。
HVLZnt是在C-末端具有ZE3抗原但没有表位标签的单链融合体，它是通过用BsaI-SacI消化来自pBYKEMd2-VHLVK的主链并与源自pBYKEMd-HZE3的插入片段连接而创建的。该构建体命名为pBYKEMd2-HVLZnt。
ZHVLnt是单链RIC，其ZE3抗原与抗体N-末端连接，没有表位标签，通过以下连接创建。使用35S-F和VH-BsaS-R引物，由pBYR11eMa-BAZE3-Hgp371扩增含有ZE3抗原和VH片段的插入片段。在5'端对VH-BsaS-R引物进行末端剪裁以包括一个BsaI位点，其在用BsaI消化时导致SpeI突出末端。用XhoI-BsaI消化该PCR片段，并与由pBYKEMd2-VHLVK获得的主链XhoI-SpeI片段连接。最终构建体命名为pBYKEMd2-ZHVLnt。
ZHVLe是具有N-末端融合的ZE3抗原和表位标签的单链抗体RIC，通过两片段连接创建。用BsaI-SacI消化pBYKEMd2-ZHVLnt以产生含有N-末端ZE3抗原和HVL构建体的载体片段。含有6D8表位标签的插入片段源自pBYR11eMa-BAZE3-Hgp371的BsaI-SacI消化。最终构建体命名为pBYKEMd2-ZHVLe。
ZHVLhx是具有N-末端融合的ZE3抗原和促进六聚体形成的点突变的单链抗体，通过两片段连接获得。通过pBYKEMd2-ZHVLnt的BsaI-SacI消化获得载体片段，插入片段源自pBYKEMd2-HVL-Hx的BsaI-SacI消化。最终构建体命名为pBYKEMd2-ZHVLhx。
下面列出了本文所述的具体构建体：
·pBYR11em-h6D8ZE3(SEQ ID NO.21中列出的序列)
·pBYR11eMa-BAZE3-Hgp371(SEQ ID NO.22中列出的序列)
·pBYR11eMa-BAZE3-H(SEQ ID NO.23中列出的序列)
·pBYKEMd-HZE3(SEQ ID NO.24中列出的序列)
·pBYKEMd-ZE3H(SEQ ID NO.25中列出的序列)
·pBYKEMd-ZE3Hx(SEQ ID NO.26中列出的序列)
·pBYKEMd-HVLZe(SEQ ID NO.27中列出的序列)
·pBYKEMd2-HVL-Hx(SEQ ID NO.28中列出的序列)
·pBYKEMd2-HVLZnt(SEQ ID NO.29中列出的序列)
·pBYKEMd2-ZHVLnt(SEQ ID NO.30中列出的序列)
·pBYKEMd2-ZHVLe(SEQ ID NO.31中列出的序列)
·pBYKEMd2-ZHVLhx(SEQ ID NO.32中列出的序列)
·pBYKEMd-6D8K(SEQ ID NO.33中列出的序列)
2.本氏烟草叶的农杆菌渗入
通过电穿孔将双元载体分别引入根癌农杆菌(Agrobacterium tumefaciens)EHA105中。所得菌株通过限制性消化或PCR验证，在30℃生长过夜，并用于渗入保持在23-25℃的5至6周龄本氏烟草叶。简要地，通过以5000g离心5min沉淀细菌，然后将细菌重悬于渗入缓冲液(10mM 2-(N-吗啉代)乙磺酸(MES)，pH 5.5和10mM MgSO4)中至OD600＝0.2，除非另有描述。使用不带针头的注射器通过小孔将所得细菌悬浮液注射到叶中(Huang和Mason，2004)。为了评估糖基化的影响，采用了木糖基转移酶和岩藻糖基转移酶被沉默的转基因植物。以5DPI收获植物组织。
3.蛋白质提取和纯化
根据制造商的说明，使用Bullet Blender机器(Next Advance,Averill Park,NY)通过用1:5(w:v)冰冷提取缓冲液(25mM Tris-HCl,pH 8.0,125mM NaCl,3mM EDTA,0.1％Triton X-100,10mg/mL抗坏血酸钠,0.3mg/mL苯甲基磺酰氟)匀浆农杆菌渗入的叶样品来提取粗蛋白。在室温或4℃旋转匀浆化组织30min。通过在4℃以13000g离心15min来澄清植物粗提物，并通过SDS-PAGE或ELISA分析上清液。可选地，为了评估原始匀浆中蛋白质的溶解度，将沉淀物指定为不溶部分，在100℃用SDS样品缓冲液处理10min，然后上样到SDS-PAGE上。
通过蛋白G亲和层析纯化IgG变体，包括HVLZe。将农杆菌渗入的叶与1:3(w:v)冰冷提取缓冲液(25mM Tris-HCl,pH 8.0,125mM NaCl,3mM EDTA,0.1％Triton X-100,10mg/mL抗坏血酸钠,0.3mg/mL苯基甲基磺酰氟)混合，在4℃搅拌30min，并过滤通过神奇滤布(miracloth)。为了沉淀内源性植物蛋白，边搅拌边用1M磷酸将pH值降至4.5，持续5min，然后用2M tris碱将pH值升至7.6。以16,000g离心20min后，按照制造商的说明将澄清的提取物上样到Pierce蛋白G柱(Thermo Fisher Scientific,Waltham,MA,USA)上。用100mM甘氨酸(pH 2.5)将纯化的蛋白质直接洗脱到含有1M Tris-HCl pH 8.0的收集管中，以中和洗脱缓冲液。以类似方式纯化HVL和ZHVLhx构建体；然而，跳过酸沉淀步骤。
通过金属亲和层析纯化从pBYe3R2K2Mc-BAZE3表达的ZE3-His。如上所述提取蛋白质，但没有酸沉淀。根据制造商的说明，将经澄清的提取物加载到含有TALON金属亲和树脂(Metal Affinity Resin,BD Clontech,Mountain View,CA)的柱上。用PBS洗涤柱并用洗脱缓冲液(PBS,150mM咪唑,pH 7.4)洗脱。将峰ZE3洗脱液合并，用PBS透析，并通过SDS-PAGE和蛋白质印迹分析。
4.SDS-PAGE和蛋白质印迹
将植物蛋白提取物或纯化蛋白样品与SDS样品缓冲液(50mM Tris-HCl,pH6.8,2％SDS,10％甘油,0.02％溴酚蓝)混合，并在4-15％无染色聚丙烯酰胺凝胶(Bio-Rad,Hercules,CA,USA)上分离。对于还原条件，加入0.5M DTT，在上样前煮沸样品10min。在紫外光下对聚丙烯酰胺凝胶进行可视化和成像，然后转移到PVDF膜上。对于IgG检测，将蛋白质转移膜在4℃用PBST(含0.05％tween-20的PBS)中的5％奶粉封闭过夜，并用山羊抗人IgG-HRP(Sigma-Aldrich,St.Louis,MO,USA，在1％PBSTM中1:5000稀释)探测。用ECL试剂(Amersham,Little Chalfont,United Kingdom)检测结合的抗体。
5.C1q结合
在37℃用PBS中的15μg/ml人补体C1q包被96孔高结合聚苯乙烯板(Corning Inc,Corning,NY,USA)2h。将板用PBST洗涤3次，然后用PBST中的5％奶粉封闭15分钟。用PBST洗涤3次后，以0.1mg/ml的10倍连续稀释液加入纯化的人IgG(Southern Biotech,Birmingham,AL,USA)和纯化的IgG-ZE3融合体，并在37℃孵育1.5h。用PBST洗涤3次后，通过在37℃与1:1000多克隆山羊抗人IgG-HRP(Southern Biotech,Birmingham,AL,USA)孵育1小时来检测结合的IgG。将板用PBST洗涤4次，用TMB底物(Thermo Fisher Scientific,Waltham,MA,USA)显色，用1M HCl停止，在450nm处读取吸光度。
6.6D8表位结合
为了测试HVL与6D8表位标签结合的能力，将900ng纯化的6D8表位标签化的登革热共有包膜结构域III与96孔高结合聚苯乙烯板(Corning Inc,Corning,NY,USA)结合。在37℃孵育1小时后，将板用PBST洗涤三次，并用PBST中的5％奶粉封闭30分钟。然后用PBST洗涤板三次，并将纯化的HLV或全长6D8抗体的各种稀释液加入板中。将板在37℃孵育1小时，用PBST洗涤三次，并用1:2000稀释度的偶联HRP的小鼠抗人IgG(仅Fc)(Southern Biotech,Birmingham,AL,USA)抗体检测。然后，将板用PBST彻底清洗并用TMB底物(Thermo FisherScientific,Waltham,MA,USA)显色。在450nm处读取吸光度。
7.蔗糖梯度密度离心
将每个IgG融合体的纯化样品(100μl)上样到不连续的蔗糖梯度上，所述梯度由2.0ml微量离心管中350μl PBS中的5、10、15、20和25％蔗糖的层组成，并在4℃以21000g离心16h。将级分收集并通过SDS-PAGE分析，然后在无染色凝胶(Bio-Rad,Hercules,CA,USA)上进行可视化。使用ImageJ软件确定每个级分的相对条带强度，任意指定峰条带的值为1。
8.小鼠的免疫和样品采集
所有动物均按照动物福利法和亚利桑那州立大学IACUC进行处理。用纯化的IgG融合变体皮下免疫6-8周龄的雌性BALB/C小鼠。在所有治疗组中，抗原的总重量设定为递送等效的8μg ZE3。在第0天和第14天给药。如Santi等人，2008中所述，通过第0、14和28天颌下采血进行血清收集。
9.抗体测量
通过ELISA测量小鼠抗体滴度。植物表达的6-His标签化的ZE3以50ng/孔与96孔高结合聚苯乙烯板(Corning Inc,Corning,NY,USA)结合，并用PBST中的5％脱脂奶粉封闭板。用PBST(含0.05％Tween 20的PBS)洗涤孔后，用1％PBSTM(含1％脱脂奶粉的PBST)稀释小鼠血清并孵育。通过与多克隆山羊抗小鼠IgG-辣根过氧化物酶偶联物(Sigma-Aldrich,St.Louis,MO,USA)孵育来检测小鼠抗体。该板用TMB底物(Thermo Fisher Scientific,Waltham,MA,USA)显色，用1M HCl停止，在450nm处读取吸光度。终点滴度取为最低稀释度的倒数，所述最低稀释度产生的OD450读数是PBS用作样品产生的背景的两倍。从在1％PBSTM中1:100稀释的血清测量IgG2a抗体，并用IgG2a辣根过氧化物酶偶联物(Santa CruzBiotechnology,Dallas,TX,USA)检测。
通过引用方式引用和并入的参考文献
Diamos,A.G.,Larios,D.,Brown,L.,Kilbourne,J.,Kim,H.S.,Saxena,D.等人(2019)Vaccine synergy with virus-like particle and immune complex platformsfor delivery of human papillomavirus L2 antigen.37,137-144.
Huang Z,Phoolcharoen W,Lai H,Piensook K,Cardineau G,Zeitlin L,WhaleyKJ,Arntzen CJ,Mason HS,Chen Q.(2010)High-level rapid production of full-sizemonoclonal antibodies in plants by a single-vector DNA repliconsystem.Biotechnol Bioeng.106(1):9-17.
Santi,L.,Batchelor,L.,Huang,Z.,Hjelm,B.,Kilbourne,J.,Arntzen,C.J.等人(2008)An efficient plant viral expression system generating orallyimmunogenic Norwalk virus-like particles.Vaccine,26,1846-1854.
Wilson,J.A.,Hevey,M.,Bakken,R.,Guest,S.,Bray,M.,Schmaljohn,A.L.和Hart,M.K.(2000)Epitopes involved in antibody-mediated protection from Ebolavirus.Science,287,1664-6.
序列表
<110> 亚利桑那州立大学董事会
<120> 用于无佐剂诱导免疫应答的IgG变体
<130> 11157.054PCT
<150> 62/980,012
<151> 2020-02-21
<160> 79
<170> PatentIn version 3.5
<210> 1
<211> 6
<212> PRT
<213> 人工序列
<220>
<223> 表位
<400> 1
Tyr Lys Leu Asp Ile Ser
1 5
<210> 2
<211> 9
<212> PRT
<213> 人工序列
<220>
<223> 表位
<400> 2
Val Tyr Lys Leu Asp Ile Ser Glu Ala
1 5
<210> 3
<211> 9
<212> PRT
<213> 人工序列
<220>
<223> 表位
<400> 3
Val Tyr Lys Leu Asp Ile Ser Glu Ala
1 5
<210> 4
<211> 7
<212> PRT
<213> 人工序列
<220>
<223> 表位
<400> 4
Tyr Lys Leu Asp Ile Ser Glu
1 5
<210> 5
<211> 101
<212> PRT
<213> 人工序列
<220>
<223> CH1结构域
<400> 5
Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr
1 5 10 15
Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro
20 25 30
Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val
35 40 45
His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser
50 55 60
Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile
65 70 75 80
Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val
85 90 95
Glu Pro Lys Ser Cys
100
<210> 6
<211> 120
<212> PRT
<213> 人工序列
<220>
<223> CH2结构域
<400> 6
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly
1 5 10 15
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
20 25 30
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His
35 40 45
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
50 55 60
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
65 70 75 80
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly
85 90 95
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Ala Pro Ile Glu
100 105 110
Lys Thr Ile Ser Lys Ala Lys Gly
115 120
<210> 7
<211> 106
<212> PRT
<213> 人工序列
<220>
<223> CH3结构域
<400> 7
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
1 5 10 15
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
20 25 30
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
35 40 45
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
50 55 60
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
65 70 75 80
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
85 90 95
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
100 105
<210> 8
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 8
gcgggatcca agggcgtgtc atactcc 27
<210> 9
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<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 9
acagagctct taagtgctac cactcctgtg 30
<210> 10
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 10
ctagtgttta caagctggac atatctgagg cataagagct 40
<210> 11
<211> 32
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 11
cttatgcctc agatatgtcc agcttgtaaa ca 32
<210> 12
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 12
tttgagctct tactcagata tgtccagctt gtaaac 36
<210> 13
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 13
aatcccacta tccttcgc 18
<210> 14
<211> 34
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 14
ctagttacaa gctggacata tctgagtaag agct 34
<210> 15
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 15
cttactcaga tatgtccagc ttgtaa 26
<210> 16
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 16
ctagttacaa gctggacata tcttaagagc t 31
<210> 17
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 17
cttaagatat gtccagcttg taa 23
<210> 18
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 18
aagcttgttg ttgtgactcc gag 23
<210> 19
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 19
cggactagta gctgaagaca ctgtgac 27
<210> 20
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 20
cgtgctagcc ttgatctcca ctttggtc 28
<210> 21
<211> 17977
<212> DNA
<213> 人工序列
<220>
<223> pBYR11eM-h6D8ZE3
<400> 21
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctagca ccaaaggtcc atcggtcttt ccactggcac 3840
cttcttccaa gagtacttct ggaggcacag ctgcactggg ttgtcttgtc aaggactact 3900
ttccagaacc tgttacggtt tcgtggaact caggtgctct gaccagtgga gtgcacacct 3960
ttccagctgt tcttcagtcc tcaggattgt attctcttag cagtgttgtg actgttccat 4020
cctcaagctt gggcactcag acctacatct gcaatgtgaa tcacaaaccc agcaacacca 4080
aggttgacaa gaaagttgag cccaagtctt gtgacaagac tcatacgtgt ccaccgtgcc 4140
cagcacctga acttcttgga ggaccgtcag tcttcttgtt tcctccaaag cctaaggata 4200
ccttgatgat ctccaggact cctgaagtca catgtgtagt tgtggatgtg agccatgaag 4260
atcctgaggt gaagttcaac tggtatgtgg atggtgtgga agtgcacaat gccaagacaa 4320
agccgagaga ggaacagtac aacagcacgt acagggttgt ctcagttctc actgttctcc 4380
atcaagattg gttgaatggc aaagagtaca agtgcaaggt ctccaacaaa gccctcccag 4440
cccccattga gaagaccatt tccaaagcga aagggcaacc ccgtgaacca caagtgtaca 4500
cacttcctcc atctcgcgat gaactgacca agaaccaggt cagcttgact tgcctggtga 4560
aaggcttcta tccctctgac atagctgtag agtgggagag caatgggcaa ccggagaaca 4620
actacaagac tacacctccc gttctcgatt ctgacggctc cttcttcctc tacagcaagc 4680
tcacagtgga caagagcagg tggcaacaag ggaatgtctt ctcatgctcc gtgatgcatg 4740
aggctcttca caatcactac acacagaaga gtctctcctt gtctccgggt aaaggaggtg 4800
gcggatcagg tggaggcggt tcaggcggag gtggatccaa gggcgtgtca tactccttgt 4860
gtaccgctgc cttcacattc accaagatcc cggctgaaac actccacgga accgttaccg 4920
tggaggtcca atacgccggt acagatggac cttgcaaggt tccagctcag atggcggtgg 4980
acatgcaaac tcttacccca gttggaaggt tgattaccgc taaccccgtt atcactgaaa 5040
gcactgagaa ctctaagatg atgttggaac ttgatccacc attcggtgac tcttacattg 5100
tcattggtgt gggagagaag aagatcaccc accactggca caggagtggt agcactagtc 5160
ataacactcc tgtttacaag ctggacatat ctgaggcaac tcaataagag ctcaaagcag 5220
aatgctgagc taaaagaaag gctttttcca ttttcgagag acaatgagaa aagaagaaga 5280
agaagaagaa gaagaagaag aagaaaagag taaataataa agccccacag gaggcgaagt 5340
tcttgtagct ccatgttatc taagttattg atattgtttg ccctatattt tatttctgtc 5400
attgtgtatg ttttgttcag tttcgagaat tctcgattaa aaatcccaat tatatttggt 5460
ctaatttagt ttggtattga gtaaaacaaa ttcgaaccaa accaaaatat aaatatatag 5520
tttttatata tatgccttta agacttttta tagaattttc tttaaaaaat atctagaaat 5580
atttgcgact cttctggcat gtaatatttc gttaaatatg aagtgctcca tttttattaa 5640
ctttaaataa ttggttgtac gatcactttc ttatcaagtg ttactaaaat gcgtcaatct 5700
ctttgttctt ccatattcat atgtcaaaat ctatcaaaat tcttatatat ctttttcgaa 5760
tttgaagtga aatttcgata atttaaaatt aaatagaaca tatcattatt taggtatcat 5820
attgattttt atacttaatt actaaatttg gttaactttg aaagtgtaca tcaacgaaaa 5880
attagtcaaa cgactaaaat aaataaatat catgtgttat taagaaaatt ctcctataag 5940
aatattttaa tagatcatat gtttgtaaaa aaaattaatt tttactaaca catatattta 6000
cttatcaaaa atttgacaaa gtaagattaa aataatattc atctaacaaa aaaaaaacca 6060
gaaaatgctg aaaacccggc aaaaccgaac caatccaaac cgatatagtt ggtttggttt 6120
gattttgata taaaccgaac caactcggtc catttgcacc cctaatcata atagctttaa 6180
tatttcaaga tattattaag ttaacgttgt caatatcctg gaaattttgc aaaatgaatc 6240
aagcctatat ggctgtaata tgaatttaaa agcagctcga tgtggtggta atatgtaatt 6300
tacttgattc taaaaaaata tcccaagtat taataatttc tgctaggaag aaggttagct 6360
acgatttaca gcaaagccag aatacaaaga accataaagt gattgaagct cgaaatatac 6420
gaaggaacaa atatttttaa aaaaatacgc aatgacttgg aacaaaagaa agtgatatat 6480
tttttgttct taaacaagca tcccctctaa agaatggcag ttttcctttg catgtaacta 6540
ttatgctccc ttcgttacaa aaattttgga ctactattgg gaacttcttc tgaaaatagt 6600
ggtaccgagt gtacttcaag tcagttggaa atcaataaaa tgattatttt atgaatatat 6660
ttcattgtgc aagtagatag aaattacata tgttacataa cacacgaaat aaacaaaaaa 6720
acacaatcca aaacaaacac cccaaacaaa ataacactat atatatcctc gtatgaggag 6780
aggcacgttc agtgactcga cgattcccga gcaaaaaaag tctccccgtc acacatatag 6840
tgggtgacgc aattatcttc aaagtaatcc ttctgttgac ttgtcattga taacatccag 6900
tcttcgtcag gattccaaag aattatagaa gggatcggtc aacatggtgg agcacgacac 6960
acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac 7020
ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca 7080
ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa 7140
aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc 7200
cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg 7260
atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt 7320
ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct 7380
cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg 7440
ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga 7500
cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc 7560
aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 7620
acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 7680
gaggacctcg agtattttta caacaattac caacaacaac aaacaacaaa caacattaca 7740
attactattt acaatctaga acaatgggat ggtcttgcat cattctcttc ttggtagcca 7800
cagctacagg tgtccactcc gatgttttga tgactcaaag ccctctctca cttcctgtga 7860
ctcttggaca gcccgcatcc atatcttgca gatctagtca gagtattgtt catagtaacg 7920
gcaacaccta cttggaatgg tatctgcaga aaccaggcca gtctccaaag cttctgatct 7980
acaaggcttc caatcgtttc tctggtgtcc cagacaggtt tagtggcagt ggatcaggga 8040
ctgacttcac attgaagatc agcagagttg aggctgaaga tgcgggagtg tactattgtc 8100
ttcaaggttc acatgttccg tcaacgtttg gaggtgggac caaagtggag atcaagactg 8160
ttgcggcgcc atctgtcttc atctttcctc catctgatga acaactcaag tctggaactg 8220
cttctgttgt gtgccttctg aacaacttct atcctagaga agccaaagta cagtggaagg 8280
ttgacaatgc tcttcaatca ggtaactccc aggagagtgt cacagagcaa gattccaagg 8340
attccaccta cagcctctca agtaccttga cgttgagcaa ggcagactat gagaaacaca 8400
aagtgtacgc atgcgaagtc actcatcagg gcctgtcatc acccgtgaca aagagcttca 8460
acaggggaga gtgttaggta ccgagctcga agtgacatca caaagttgaa ggtaataaag 8520
ccaaattaat taagacattt tcataatgat gtcaagaatg caaagcaaat tgcataactg 8580
cctttatgca aaacattaat ataatataaa ttataaagaa ctgcgctctc tgcttcttat 8640
tttcttagct tcatttatta gtcactagct gttcagaatt ttcagtatct tttgatatta 8700
ctaagaacct aatcacacaa tgtatattct tatgcaggaa aagcagaatg ctgagctaaa 8760
agaaaggctt tttccatttt cgagagacaa tgagaaaaga agaagaagaa gaagaagaag 8820
aagaagaaga aaagagtaaa taataaagcc ccacaggagg cgaagttctt gtagctccat 8880
gttatctaag ttattgatat tgtttgccct atattttatt tctgtcattg tgtatgtttt 8940
gttcagtttc gatctccttg caaaatgcag agattatgag atgaataaac taagttatat 9000
tattatacgt gttaatattc tcctcctctc tctagctagc cttttgtttt ctctttttct 9060
tatttgattt tctttaaatc aatccatttt aggagagggc cagggagtga tccagcaaaa 9120
catgaagatt agaagaaact tccctctttt ttttcctgaa aacaatttaa cgtcgagatt 9180
tatctctttt tgtaatggaa tcatttctac agttatgacg aattctcgat taaaaatccc 9240
aattatattt ggtctaattt agtttggtat tgagtaaaac aaattcgaac caaaccaaaa 9300
tataaatata tagtttttat atatatgcct ttaagacttt ttatagaatt ttctttaaaa 9360
aatatctaga aatatttgcg actcttctgg catgtaatat ttcgttaaat atgaagtgct 9420
ccatttttat taactttaaa taattggttg tacgatcact ttcttatcaa gtgttactaa 9480
aatgcgtcaa tctctttgtt cttccatatt catatgtcaa aatctatcaa aattcttata 9540
tatctttttc gaatttgaag tgaaatttcg ataatttaaa attaaataga acatatcatt 9600
atttaggtat catattgatt tttatactta attactaaat ttggttaact ttgaaagtgt 9660
acatcaacga aaaattagtc aaacgactaa aataaataaa tatcatgtgt tattaagaaa 9720
attctcctat aagaatattt taatagatca tatgtttgta aaaaaaatta atttttacta 9780
acacatatat ttacttatca aaaatttgac aaagtaagat taaaataata ttcatctaac 9840
aaaaaaaaaa ccagaaaatg ctgaaaaccc ggcaaaaccg aaccaatcca aaccgatata 9900
gttggtttgg tttgattttg atataaaccg aaccaactcg gtccatttgc acccctaatc 9960
ataatagctt taatatttca agatattatt aagttaacgt tgtcaatatc ctggaaattt 10020
tgcaaaatga atcaagccta tatggctgta atatgaattt aaaagcagct cgatgtggtg 10080
gtaatatgta atttacttga ttctaaaaaa atatcccaag tattaataat ttctgctagg 10140
aagaaggtta gctacgattt acagcaaagc cagaatacaa agaaccataa agtgattgaa 10200
gctcgaaata tacgaaggaa caaatatttt taaaaaaata cgcaatgact tggaacaaaa 10260
gaaagtgata tattttttgt tcttaaacaa gcatcccctc taaagaatgg cagttttcct 10320
ttgcatgtaa ctattatgct cccttcgtta caaaaatttt ggactactat tgggaacttc 10380
ttctgaaaat agtggtaccg agtgtacttc aagtcagttg gaaatcaata aaatgattat 10440
tttatgaata tatttcattg tgcaagtaga tagaaattac atatgttaca taacacacga 10500
aataaacaaa aaaacacaat ccaaaacaaa caccccaaac aaaataacac tatatatatc 10560
ctcgtatgag gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa aagtctcccc 10620
gtcacacata tagtgggtga cgcaattatc ttcaaagtaa tccttctgtt gacttgtcat 10680
tgataacatc cagtcttcgt caggattgca aagaattata gaagggatcc caccttttat 10740
tttcttcttt tttccatatt tagggttgac agtgaaatca gactggcaac ctattaattg 10800
cttccacaat gggacgaact tgaaggggat gtcgtcgatg atattatagg tggcgtgttc 10860
atcgtagttg gtgaagtcga tggtcccgtt ccagtagttg tgtcgcccga gacttctagc 10920
ccaggtggtc tttccggtac gagttggtcc gcagatgtag aggctggggt gtctgacccc 10980
agtccttccc tcatcctggt tagatcggcc atccactcaa ggtcagattg tgcttgatcg 11040
taggagacag gatgtatgaa agtgtaggca tcgatgctta catgatatag gtgcgtctct 11100
ctccagttgt gcagatcttc gtggcagcgg agatctgatt ctgtgaaggg cgacacgtac 11160
tgctcaggtt gtggaggaaa taatttgttg gctgaatatt ccagccattg aagctttgtt 11220
gcccattcat gagggaactc ttctttgatc atgtcaagat actcctcctt agacgttgca 11280
gtctggataa tagttcgcca tcgtgcgtca gatttgcgag gagacacctt atgatctcgg 11340
aaatctcctc tggttttaat atctccgtcc tttgatatgt aatcaaggac ttgtttagag 11400
tttctagctg gctggatatt agggtgattt ccttcaaaat cgaaaaaaga aggatcccta 11460
atacaaggtt ttttatcaag ctggataaga gcatgatagt gggtagtgcc atcttgatga 11520
agctcagaag caacaccaag gaagaaaata agaaaaggtg tgagtttctc ccagagaaac 11580
tggaataaat catctctttg agatgagcac ttggggtagg taaggaaaac atatttagat 11640
tggagtctga agttcttgct agcagaaggc atgttgttgt gactccgagg ggttgcctca 11700
aactctatct tataaccggc gtggaggcat ggaggcaagg gcattttggt aatttaagta 11760
gttagtggaa aatgacgtca tttacttaaa gacgaagtct tgcgacaagg ggggcccacg 11820
ccgaatttta atattaccgg cgtggcccca ccttatcgcg agtgctttag cacgagcggt 11880
ccagatttaa agtagaaaag ttcccgccca ctagggttaa aggtgttcac actataaaag 11940
catatacgat gtgatggtat ttgatggagc gtatattgta tcaggtattt ccgtcggata 12000
cgaattattc gtacggccgg accggtcccc taggccggcc aattcgagat cggccgcggc 12060
tgagtggctc cttcaatcgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg 12120
cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cagattgtcg 12180
tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 12240
gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg 12300
ttcgtccatt tgtatgtgca tgccaaccac agggttcccc agatctggcg ccggccagcg 12360
agacgagcaa gattggccgc cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc 12420
aggcaaattg caccaacgca tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt 12480
tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg ccgatgccga 12540
cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tatgttgggt ttcacgtctg 12600
gcctccggag actgtcatac gcgtaaaaag gccgcgttgc tggcgttttt ccataggctc 12660
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 12720
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 12780
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 12840
catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 12900
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 12960
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 13020
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 13080
actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 13140
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 13200
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 13260
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 13320
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 13380
atatatgagt aaacttggtc tgcagttgcc atgttttacg gcagtgagag cagagatagc 13440
gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg 13500
gacagctgat agacacagaa gccactggag cacctcaaaa acaccatcat acactaaatc 13560
agtaagttgg cagcatcacc cataattgtg gtttcaaaat cggctccgtc gatactatgt 13620
tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga 13680
atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta 13740
aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt 13800
gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa 13860
ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta 13920
taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa 13980
gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat 14040
gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa 14100
gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg 14160
tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga 14220
tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga 14280
gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga 14340
cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg 14400
gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga 14460
ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga 14520
ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc 14580
gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct 14640
ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca 14700
agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg 14760
ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc 14820
acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg 14880
accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa 14940
ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga 15000
tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc 15060
tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt 15120
tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg 15180
ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca 15240
cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca 15300
cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga 15360
aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga 15420
tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt 15480
tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt 15540
gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt 15600
cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg 15660
tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg 15720
tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat 15780
gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc 15840
accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca 15900
cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg 15960
gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg 16020
tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag 16080
cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta 16140
cgaactgccg ataaacagag gattaaaatt gacaattcaa tggcaaggac tgccagcgct 16200
gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg 16260
cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg 16320
tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc 16380
aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga 16440
ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact 16500
ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg 16560
tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta 16620
aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg 16680
agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc 16740
cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc 16800
gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc 16860
cgccagccca gcggcgaggg caaccagccc ggtgagcgtc gcaaaggcgc tcggtcttgc 16920
cttgctcgtc gagatctggg gtcgatcagc cggggatgca tcaggccgac agtcggaact 16980
tcgggtcccc gacctgtacc attcggtgag caatggatag gggagttgat atcgtcaacg 17040
ttcacttcta aagaaatagc gccactcagc ttcctcagcg gctttatcca gcgatttcct 17100
attatgtcgg catagttctc aagatcgaca gcctgtcacg gttaagcgag aaatgaataa 17160
gaaggctgat aattcggatc tctgcgaggg agatgatatt tgatcacagg cagcaacgct 17220
ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc 17280
agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca 17340
acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt 17400
gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta 17460
aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt 17520
ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg 17580
agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat 17640
ggtggttccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg 17700
agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 17760
gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt 17820
tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 17880
agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 17940
gcgggcgcca ttcaggctgc gcaactgttg ggaaggg 17977
<210> 22
<211> 17686
<212> DNA
<213> 人工序列
<220>
<223> pBYR11eMa-BAZE3-Hgp371
<400> 22
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttcttga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctagcacc aaaggtccat 4200
cggtctttcc actggcacct tcttccaaga gtacttctgg aggcacagct gcactgggtt 4260
gtcttgtcaa ggactacttt ccagaacctg ttacggtttc gtggaactca ggtgctctga 4320
ccagtggagt gcacaccttt ccagctgttc ttcagtcctc aggattgtat tctcttagca 4380
gtgttgtgac tgttccatcc tcaagcttgg gcactcagac ctacatctgc aatgtgaatc 4440
acaaacccag caacaccaag gttgacaaga aagttgagcc caagtcttgt gacaagactc 4500
atacgtgtcc accgtgccca gcacctgaac ttcttggagg accgtcagtc ttcttgtttc 4560
ctccaaagcc taaggatacc ttgatgatct ccaggactcc tgaagtcaca tgtgtagttg 4620
tggatgtgag ccatgaagat cctgaggtga agttcaactg gtatgtggat ggtgtggaag 4680
tgcacaatgc caagacaaag ccgagagagg aacagtacaa cagcacgtac agggttgtct 4740
cagttctcac tgttctccat caagattggt tgaatggcaa agagtacaag tgcaaggtct 4800
ccaacaaagc cctcccagcc cccattgaga agaccatttc caaagcgaaa gggcaacccc 4860
gtgaaccaca agtgtacaca cttcctccat ctcgcgatga actgaccaag aaccaggtca 4920
gcttgacttg cctggtgaaa ggcttctatc cctctgacat agctgtagag tgggagagca 4980
atgggcaacc ggagaacaac tacaagacta cacctcccgt tctcgattct gacggctcct 5040
tcttcctcta cagcaagctc acagtggaca agagcaggtg gcaacaaggg aatgtcttct 5100
catgctccgt gatgcatgag gctcttcaca atcactacac acagaagagt ctctccttgt 5160
ctccgggtaa aggaggtggc ggatcaggtg gaggcggttc aggcggaggt ggatcccata 5220
acactcctgt ttacaagctg gacatatctg aggcaactca ataagagctc gaagtgacat 5280
cacaaagttg aaggtaataa agccaaatta attaagacat tttcataatg atgtcaagaa 5340
tgcaaagcaa attgcataac tgcctttatg caaaacatta atataatata aattataaag 5400
aactgcgctc tctgcttctt attttcttag cttcatttat tagtcactag ctgttcagaa 5460
ttttcagtat cttttgatat tactaagaac ctaatcacac aatgtatatt cttatgcagg 5520
aaaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac aatgagaaaa 5580
gaagaagaag aagaagaaga agaagaagaa gaaaagagta aataataaag ccccacagga 5640
ggcgaagttc ttgtagctcc atgttatcta agttattgat attgtttgcc ctatatttta 5700
tttctgtcat tgtgtatgtt ttgttcagtt tcgatctcct tgcaaaatgc agagattatg 5760
agatgaataa actaagttat attattatac gtgttaatat tctcctcctc tctctagcta 5820
gccttttgtt ttctcttttt cttatttgat tttctttaaa tcaatccatt ttaggagagg 5880
gccagggagt gatccagcaa aacatgaaga ttagaagaaa cttccctctt ttttttcctg 5940
aaaacaattt aacgtcgaga tttatctctt tttgtaatgg aatcatttct acagttatga 6000
cgaattgtac atcaacgaaa aattagtcaa acgactaaaa taaataaata tcatgtgtta 6060
ttaagaaaat tctcctataa gaatatttta atagatcata tgtttgtaaa aaaaattaat 6120
ttttactaac acatatattt acttatcaaa aatttgacaa agtaagatta aaataatatt 6180
catctaacaa aaaaaaaacc agaaaatgct gaaaacccgg caaaaccgaa ccaatccaaa 6240
ccgatatagt tggtttggtt tgattttgat ataaaccgaa ccaactcggt ccatttgcac 6300
ccctaatcat aatagcttta atatttcaag atattattaa gttaacgttg tcaatatcct 6360
ggaaattttg caaaatgaat caagcctata tggctgtaat atgaatttaa aagcagctcg 6420
atgtggtggt aatatgtaat ttacttgatt ctaaaaaaat atcccaagta ttaataattt 6480
ctgctaggaa gaaggttagc tacgatttac agcaaagcca gaatacaaag aaccataaag 6540
tgattgaagc tcgaaatata cgaaggaaca aatattttta aaaaaatacg caatgacttg 6600
gaacaaaaga aagtgatata ttttttgttc ttaaacaagc atcccctcta aagaatggca 6660
gttttccttt gcatgtaact attatgctcc cttcgttaca aaaattttgg actactattg 6720
ggaacttctt ctgaaaatag tggtaccgag tgtacttcaa gtcagttgga aatcaataaa 6780
atgattattt tatgaatata tttcattgtg caagtagata gaaattacat atgttacata 6840
acacacgaaa taaacaaaaa aacacaatcc aaaacaaaca ccccaaacaa aataacacta 6900
tatatatcct cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa 6960
gtctccccgt cacacatata gtgggtgacg caattatctt caaagtaatc cttctgttga 7020
cttgtcattg ataacatcca gtcttcgtca ggattccaaa gaattataga agggatcggt 7080
caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga 7140
agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt 7200
ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta 7260
caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg 7320
tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac 7380
gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc 7440
caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag 7500
ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa 7560
gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat 7620
cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 7680
cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc 7740
cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata 7800
aggaagttca tttcatttgg agaggacctc gagaaacaaa caaaatcaac aaatatagaa 7860
aataacgcat ttccaattct ttgaaatttc tgcaacatct agaacaatgg gatggtcttg 7920
catcattctc ttcttggtag ccacagctac aggtgtccac tccgatgttt tgatgactca 7980
aagccctctc tcacttcctg tgactcttgg acagcccgca tccatatctt gcagatctag 8040
tcagagtatt gttcatagta acggcaacac ctacttggaa tggtatctgc agaaaccagg 8100
ccagtctcca aagcttctga tctacaaggc ttccaatcgt ttctctggtg tcccagacag 8160
gtttagtggc agtggatcag ggactgactt cacattgaag atcagcagag ttgaggctga 8220
agatgcggga gtgtactatt gtcttcaagg ttcacatgtt ccgtcaacgt ttggaggtgg 8280
gaccaaagtg gagatcaaga ctgttgcggc gccatctgtc ttcatctttc ctccatctga 8340
tgaacaactc aagtctggaa ctgcttctgt tgtgtgcctt ctgaacaact tctatcctag 8400
agaagccaaa gtacagtgga aggttgacaa tgctcttcaa tcaggtaact cccaggagag 8460
tgtcacagag caagattcca aggattccac ctacagcctc tcaagtacct tgacgttgag 8520
caaggcagac tatgagaaac acaaagtgta cgcatgcgaa gtcactcatc agggcctgtc 8580
atcacccgtg acaaagagct tcaacagggg agagtgttag gtaccgagct cgaagtgaca 8640
tcacaaagtt gaaggtaata aagccaaatt aattaagaca ttttcataat gatgtcaaga 8700
atgcaaagca aattgcataa ctgcctttat gcaaaacatt aatataatat aaattataaa 8760
gaactgcgct ctctgcttct tattttctta gcttcattta ttagtcacta gctgttcaga 8820
attttcagta tcttttgata ttactaagaa cctaatcaca caatgtatat tcttatgcag 8880
gaaaagcaga atgctgagct aaaagaaagg ctttttccat tttcgagaga caatgagaaa 8940
agaagaagaa gaagaagaag aagaagaaga agaaaagagt aaataataaa gccccacagg 9000
aggcgaagtt cttgtagctc catgttatct aagttattga tattgtttgc cctatatttt 9060
atttctgtca ttgtgtatgt tttgttcagt ttcgatctcc ttgcaaaatg cagagattat 9120
gagatgaata aactaagtta tattattata cgtgttaata ttctcctcct ctctctagct 9180
agccttttgt tttctctttt tcttatttga ttttctttaa atcaatccat tttaggagag 9240
ggccagggag tgatccagca aaacatgaag attagaagaa acttccctct tttttttcct 9300
gaaaacaatt taacgtcgag atttatctct ttttgtaatg gaatcatttc tacagttatg 9360
acgaattgta catcaacgaa aaattagtca aacgactaaa ataaataaat atcatgtgtt 9420
attaagaaaa ttctcctata agaatatttt aatagatcat atgtttgtaa aaaaaattaa 9480
tttttactaa cacatatatt tacttatcaa aaatttgaca aagtaagatt aaaataatat 9540
tcatctaaca aaaaaaaaac cagaaaatgc tgaaaacccg gcaaaaccga accaatccaa 9600
accgatatag ttggtttggt ttgattttga tataaaccga accaactcgg tccatttgca 9660
cccctaatca taatagcttt aatatttcaa gatattatta agttaacgtt gtcaatatcc 9720
tggaaatttt gcaaaatgaa tcaagcctat atggctgtaa tatgaattta aaagcagctc 9780
gatgtggtgg taatatgtaa tttacttgat tctaaaaaaa tatcccaagt attaataatt 9840
tctgctagga agaaggttag ctacgattta cagcaaagcc agaatacaaa gaaccataaa 9900
gtgattgaag ctcgaaatat acgaaggaac aaatattttt aaaaaaatac gcaatgactt 9960
ggaacaaaag aaagtgatat attttttgtt cttaaacaag catcccctct aaagaatggc 10020
agttttcctt tgcatgtaac tattatgctc ccttcgttac aaaaattttg gactactatt 10080
gggaacttct tctgaaaata gtggtaccga gtgtacttca agtcagttgg aaatcaataa 10140
aatgattatt ttatgaatat atttcattgt gcaagtagat agaaattaca tatgttacat 10200
aacacacgaa ataaacaaaa aaacacaatc caaaacaaac accccaaaca aaataacact 10260
atatatatcc tcgtatgagg agaggcacgt tcagtgactc gacgattccc gagcaaaaaa 10320
agtctccccg tcacacatat agtgggtgac gcaattatct tcaaagtaat ccttctgttg 10380
acttgtcatt gataacatcc agtcttcgtc aggattgcaa agaattatag aagggatccc 10440
accttttatt ttcttctttt ttccatattt agggttgaca gtgaaatcag actggcaacc 10500
tattaattgc ttccacaatg ggacgaactt gaaggggatg tcgtcgatga tattataggt 10560
ggcgtgttca tcgtagttgg tgaagtcgat ggtcccgttc cagtagttgt gtcgcccgag 10620
acttctagcc caggtggtct ttccggtacg agttggtccg cagatgtaga ggctggggtg 10680
tctgacccca gtccttccct catcctggtt agatcggcca tccactcaag gtcagattgt 10740
gcttgatcgt aggagacagg atgtatgaaa gtgtaggcat cgatgcttac atgatatagg 10800
tgcgtctctc tccagttgtg cagatcttcg tggcagcgga gatctgattc tgtgaagggc 10860
gacacgtact gctcaggttg tggaggaaat aatttgttgg ctgaatattc cagccattga 10920
agctttgttg cccattcatg agggaactct tctttgatca tgtcaagata ctcctcctta 10980
gacgttgcag tctggataat agttcgccat cgtgcgtcag atttgcgagg agacacctta 11040
tgatctcgga aatctcctct ggttttaata tctccgtcct ttgatatgta atcaaggact 11100
tgtttagagt ttctagctgg ctggatatta gggtgatttc cttcaaaatc gaaaaaagaa 11160
ggatccctaa tacaaggttt tttatcaagc tggataagag catgatagtg ggtagtgcca 11220
tcttgatgaa gctcagaagc aacaccaagg aagaaaataa gaaaaggtgt gagtttctcc 11280
cagagaaact ggaataaatc atctctttga gatgagcact tggggtaggt aaggaaaaca 11340
tatttagatt ggagtctgaa gttcttgcta gcagaaggca tgttgttgtg actccgaggg 11400
gttgcctcaa actctatctt ataaccggcg tggaggcatg gaggcaaggg cattttggta 11460
atttaagtag ttagtggaaa atgacgtcat ttacttaaag acgaagtctt gcgacaaggg 11520
gggcccacgc cgaattttaa tattaccggc gtggccccac cttatcgcga gtgctttagc 11580
acgagcggtc cagatttaaa gtagaaaagt tcccgcccac tagggttaaa ggtgttcaca 11640
ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat caggtatttc 11700
cgtcggatac gaattattcg tacggccgga ccggtcccct aggccggcca attcgagatc 11760
ggccgcggct gagtggctcc ttcaatcgtt gcggttctgt cagttccaaa cgtaaaacgg 11820
cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc 11880
agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg 11940
taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag 12000
gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccca gatctggcgc 12060
cggccagcga gacgagcaag attggccgcc gcccgaaacg atccgacagc gcgcccagca 12120
caggtgcgca ggcaaattgc accaacgcat acagcgccag cagaatgcca tagtgggcgg 12180
tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc ataatcaggc 12240
cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt atgttgggtt 12300
tcacgtctgg cctccggaga ctgtcatacg cgtaaaaagg ccgcgttgct ggcgtttttc 12360
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 12420
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 12480
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 12540
gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 12600
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 12660
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 12720
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 12780
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 12840
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 12900
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 12960
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 13020
agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 13080
atctaaagta tatatgagta aacttggtct gcagttgcca tgttttacgg cagtgagagc 13140
agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg 13200
aacaggaggg acagctgata gacacagaag ccactggagc acctcaaaaa caccatcata 13260
cactaaatca gtaagttggc agcatcaccc ataattgtgg tttcaaaatc ggctccgtcg 13320
atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa 13380
ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg 13440
gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc 13500
accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc 13560
tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga 13620
cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct 13680
ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa 13740
tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag 13800
ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat 13860
atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact 13920
gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga 13980
tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc 14040
ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat 14100
tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc 14160
gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat 14220
caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct 14280
agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt 14340
gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg 14400
tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg 14460
acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg 14520
aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc 14580
ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg 14640
atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg 14700
tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc 14760
cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg 14820
cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc 14880
gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc 14940
tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt 15000
ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc 15060
gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg 15120
acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc 15180
agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct 15240
acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg 15300
aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc 15360
tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc 15420
gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga 15480
aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt 15540
tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat 15600
cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc 15660
gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct 15720
agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcttta ctggcatttc 15780
aggaacaagc gggcactgct cgacgcactt gcttcgctca gtatcgctcg ggacgcacgg 15840
cgcgctctac gaactgccga taaacagagg attaaaattg acaattcaat ggcaaggact 15900
gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg 15960
gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg 16020
cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg 16080
tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca 16140
aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct 16200
gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc 16260
cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa 16320
actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg 16380
ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt 16440
gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg 16500
ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc 16560
atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg caaaggcgct 16620
cggtcttgcc ttgctcgtcg agatctgggg tcgatcagcc ggggatgcat caggccgaca 16680
gtcggaactt cgggtccccg acctgtacca ttcggtgagc aatggatagg ggagttgata 16740
tcgtcaacgt tcacttctaa agaaatagcg ccactcagct tcctcagcgg ctttatccag 16800
cgatttccta ttatgtcggc atagttctca agatcgacag cctgtcacgg ttaagcgaga 16860
aatgaataag aaggctgata attcggatct ctgcgaggga gatgatattt gatcacaggc 16920
agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc 16980
aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc 17040
cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt 17100
ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat 17160
tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg 17220
tactggggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc 17280
ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc 17340
ctgtttgatg gtggttccga aatcggcaaa atcccttata aatcaaaaga atagcccgag 17400
atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 17460
aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 17520
aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 17580
ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 17640
gcgaaaggag cgggcgccat tcaggctgcg caactgttgg gaaggg 17686
<210> 23
<211> 17602
<212> DNA
<213> 人工序列
<220>
<223> pBYR11eMa-BAZE3-H
<400> 23
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttcttga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctagcacc aaaggtccat 4200
cggtctttcc actggcacct tcttccaaga gtacttctgg aggcacagct gcactgggtt 4260
gtcttgtcaa ggactacttt ccagaacctg ttacggtttc gtggaactca ggtgctctga 4320
ccagtggagt gcacaccttt ccagctgttc ttcagtcctc aggattgtat tctcttagca 4380
gtgttgtgac tgttccatcc tcaagcttgg gcactcagac ctacatctgc aatgtgaatc 4440
acaaacccag caacaccaag gttgacaaga aagttgagcc caagtcttgt gacaagactc 4500
atacgtgtcc accgtgccca gcacctgaac ttcttggagg accgtcagtc ttcttgtttc 4560
ctccaaagcc taaggatacc ttgatgatct ccaggactcc tgaagtcaca tgtgtagttg 4620
tggatgtgag ccatgaagat cctgaggtga agttcaactg gtatgtggat ggtgtggaag 4680
tgcacaatgc caagacaaag ccgagagagg aacagtacaa cagcacgtac agggttgtct 4740
cagttctcac tgttctccat caagattggt tgaatggcaa agagtacaag tgcaaggtct 4800
ccaacaaagc cctcccagcc cccattgaga agaccatttc caaagcgaaa gggcaacccc 4860
gtgaaccaca agtgtacaca cttcctccat ctcgcgatga actgaccaag aaccaggtca 4920
gcttgacttg cctggtgaaa ggcttctatc cctctgacat agctgtagag tgggagagca 4980
atgggcaacc ggagaacaac tacaagacta cacctcccgt tctcgattct gacggctcct 5040
tcttcctcta cagcaagctc acagtggaca agagcaggtg gcaacaaggg aatgtcttct 5100
catgctccgt gatgcatgag gctcttcaca atcactacac acagaagagt ctctccttgt 5160
ctccgggtaa atgaggtacc gagctcgaag tgacatcaca aagttgaagg taataaagcc 5220
aaattaatta agacattttc ataatgatgt caagaatgca aagcaaattg cataactgcc 5280
tttatgcaaa acattaatat aatataaatt ataaagaact gcgctctctg cttcttattt 5340
tcttagcttc atttattagt cactagctgt tcagaatttt cagtatcttt tgatattact 5400
aagaacctaa tcacacaatg tatattctta tgcaggaaaa gcagaatgct gagctaaaag 5460
aaaggctttt tccattttcg agagacaatg agaaaagaag aagaagaaga agaagaagaa 5520
gaagaagaaa agagtaaata ataaagcccc acaggaggcg aagttcttgt agctccatgt 5580
tatctaagtt attgatattg tttgccctat attttatttc tgtcattgtg tatgttttgt 5640
tcagtttcga tctccttgca aaatgcagag attatgagat gaataaacta agttatatta 5700
ttatacgtgt taatattctc ctcctctctc tagctagcct tttgttttct ctttttctta 5760
tttgattttc tttaaatcaa tccattttag gagagggcca gggagtgatc cagcaaaaca 5820
tgaagattag aagaaacttc cctctttttt ttcctgaaaa caatttaacg tcgagattta 5880
tctctttttg taatggaatc atttctacag ttatgacgaa ttgtacatca acgaaaaatt 5940
agtcaaacga ctaaaataaa taaatatcat gtgttattaa gaaaattctc ctataagaat 6000
attttaatag atcatatgtt tgtaaaaaaa attaattttt actaacacat atatttactt 6060
atcaaaaatt tgacaaagta agattaaaat aatattcatc taacaaaaaa aaaaccagaa 6120
aatgctgaaa acccggcaaa accgaaccaa tccaaaccga tatagttggt ttggtttgat 6180
tttgatataa accgaaccaa ctcggtccat ttgcacccct aatcataata gctttaatat 6240
ttcaagatat tattaagtta acgttgtcaa tatcctggaa attttgcaaa atgaatcaag 6300
cctatatggc tgtaatatga atttaaaagc agctcgatgt ggtggtaata tgtaatttac 6360
ttgattctaa aaaaatatcc caagtattaa taatttctgc taggaagaag gttagctacg 6420
atttacagca aagccagaat acaaagaacc ataaagtgat tgaagctcga aatatacgaa 6480
ggaacaaata tttttaaaaa aatacgcaat gacttggaac aaaagaaagt gatatatttt 6540
ttgttcttaa acaagcatcc cctctaaaga atggcagttt tcctttgcat gtaactatta 6600
tgctcccttc gttacaaaaa ttttggacta ctattgggaa cttcttctga aaatagtggt 6660
accgagtgta cttcaagtca gttggaaatc aataaaatga ttattttatg aatatatttc 6720
attgtgcaag tagatagaaa ttacatatgt tacataacac acgaaataaa caaaaaaaca 6780
caatccaaaa caaacacccc aaacaaaata acactatata tatcctcgta tgaggagagg 6840
cacgttcagt gactcgacga ttcccgagca aaaaaagtct ccccgtcaca catatagtgg 6900
gtgacgcaat tatcttcaaa gtaatccttc tgttgacttg tcattgataa catccagtct 6960
tcgtcaggat tccaaagaat tatagaaggg atcggtcaac atggtggagc acgacacact 7020
tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt 7080
tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt 7140
tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 7200
aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac 7260
gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 7320
tgataacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc 7380
agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg 7440
attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc 7500
ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag 7560
tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac 7620
cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca 7680
atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag 7740
gacctcgaga aacaaacaaa atcaacaaat atagaaaata acgcatttcc aattctttga 7800
aatttctgca acatctagaa caatgggatg gtcttgcatc attctcttct tggtagccac 7860
agctacaggt gtccactccg atgttttgat gactcaaagc cctctctcac ttcctgtgac 7920
tcttggacag cccgcatcca tatcttgcag atctagtcag agtattgttc atagtaacgg 7980
caacacctac ttggaatggt atctgcagaa accaggccag tctccaaagc ttctgatcta 8040
caaggcttcc aatcgtttct ctggtgtccc agacaggttt agtggcagtg gatcagggac 8100
tgacttcaca ttgaagatca gcagagttga ggctgaagat gcgggagtgt actattgtct 8160
tcaaggttca catgttccgt caacgtttgg aggtgggacc aaagtggaga tcaagactgt 8220
tgcggcgcca tctgtcttca tctttcctcc atctgatgaa caactcaagt ctggaactgc 8280
ttctgttgtg tgccttctga acaacttcta tcctagagaa gccaaagtac agtggaaggt 8340
tgacaatgct cttcaatcag gtaactccca ggagagtgtc acagagcaag attccaagga 8400
ttccacctac agcctctcaa gtaccttgac gttgagcaag gcagactatg agaaacacaa 8460
agtgtacgca tgcgaagtca ctcatcaggg cctgtcatca cccgtgacaa agagcttcaa 8520
caggggagag tgttaggtac cgagctcgaa gtgacatcac aaagttgaag gtaataaagc 8580
caaattaatt aagacatttt cataatgatg tcaagaatgc aaagcaaatt gcataactgc 8640
ctttatgcaa aacattaata taatataaat tataaagaac tgcgctctct gcttcttatt 8700
ttcttagctt catttattag tcactagctg ttcagaattt tcagtatctt ttgatattac 8760
taagaaccta atcacacaat gtatattctt atgcaggaaa agcagaatgc tgagctaaaa 8820
gaaaggcttt ttccattttc gagagacaat gagaaaagaa gaagaagaag aagaagaaga 8880
agaagaagaa aagagtaaat aataaagccc cacaggaggc gaagttcttg tagctccatg 8940
ttatctaagt tattgatatt gtttgcccta tattttattt ctgtcattgt gtatgttttg 9000
ttcagtttcg atctccttgc aaaatgcaga gattatgaga tgaataaact aagttatatt 9060
attatacgtg ttaatattct cctcctctct ctagctagcc ttttgttttc tctttttctt 9120
atttgatttt ctttaaatca atccatttta ggagagggcc agggagtgat ccagcaaaac 9180
atgaagatta gaagaaactt ccctcttttt tttcctgaaa acaatttaac gtcgagattt 9240
atctcttttt gtaatggaat catttctaca gttatgacga attgtacatc aacgaaaaat 9300
tagtcaaacg actaaaataa ataaatatca tgtgttatta agaaaattct cctataagaa 9360
tattttaata gatcatatgt ttgtaaaaaa aattaatttt tactaacaca tatatttact 9420
tatcaaaaat ttgacaaagt aagattaaaa taatattcat ctaacaaaaa aaaaaccaga 9480
aaatgctgaa aacccggcaa aaccgaacca atccaaaccg atatagttgg tttggtttga 9540
ttttgatata aaccgaacca actcggtcca tttgcacccc taatcataat agctttaata 9600
tttcaagata ttattaagtt aacgttgtca atatcctgga aattttgcaa aatgaatcaa 9660
gcctatatgg ctgtaatatg aatttaaaag cagctcgatg tggtggtaat atgtaattta 9720
cttgattcta aaaaaatatc ccaagtatta ataatttctg ctaggaagaa ggttagctac 9780
gatttacagc aaagccagaa tacaaagaac cataaagtga ttgaagctcg aaatatacga 9840
aggaacaaat atttttaaaa aaatacgcaa tgacttggaa caaaagaaag tgatatattt 9900
tttgttctta aacaagcatc ccctctaaag aatggcagtt ttcctttgca tgtaactatt 9960
atgctccctt cgttacaaaa attttggact actattggga acttcttctg aaaatagtgg 10020
taccgagtgt acttcaagtc agttggaaat caataaaatg attattttat gaatatattt 10080
cattgtgcaa gtagatagaa attacatatg ttacataaca cacgaaataa acaaaaaaac 10140
acaatccaaa acaaacaccc caaacaaaat aacactatat atatcctcgt atgaggagag 10200
gcacgttcag tgactcgacg attcccgagc aaaaaaagtc tccccgtcac acatatagtg 10260
ggtgacgcaa ttatcttcaa agtaatcctt ctgttgactt gtcattgata acatccagtc 10320
ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct tttattttct tcttttttcc 10380
atatttaggg ttgacagtga aatcagactg gcaacctatt aattgcttcc acaatgggac 10440
gaacttgaag gggatgtcgt cgatgatatt ataggtggcg tgttcatcgt agttggtgaa 10500
gtcgatggtc ccgttccagt agttgtgtcg cccgagactt ctagcccagg tggtctttcc 10560
ggtacgagtt ggtccgcaga tgtagaggct ggggtgtctg accccagtcc ttccctcatc 10620
ctggttagat cggccatcca ctcaaggtca gattgtgctt gatcgtagga gacaggatgt 10680
atgaaagtgt aggcatcgat gcttacatga tataggtgcg tctctctcca gttgtgcaga 10740
tcttcgtggc agcggagatc tgattctgtg aagggcgaca cgtactgctc aggttgtgga 10800
ggaaataatt tgttggctga atattccagc cattgaagct ttgttgccca ttcatgaggg 10860
aactcttctt tgatcatgtc aagatactcc tccttagacg ttgcagtctg gataatagtt 10920
cgccatcgtg cgtcagattt gcgaggagac accttatgat ctcggaaatc tcctctggtt 10980
ttaatatctc cgtcctttga tatgtaatca aggacttgtt tagagtttct agctggctgg 11040
atattagggt gatttccttc aaaatcgaaa aaagaaggat ccctaataca aggtttttta 11100
tcaagctgga taagagcatg atagtgggta gtgccatctt gatgaagctc agaagcaaca 11160
ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga gaaactggaa taaatcatct 11220
ctttgagatg agcacttggg gtaggtaagg aaaacatatt tagattggag tctgaagttc 11280
ttgctagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc tatcttataa 11340
ccggcgtgga ggcatggagg caagggcatt ttggtaattt aagtagttag tggaaaatga 11400
cgtcatttac ttaaagacga agtcttgcga caaggggggc ccacgccgaa ttttaatatt 11460
accggcgtgg ccccacctta tcgcgagtgc tttagcacga gcggtccaga tttaaagtag 11520
aaaagttccc gcccactagg gttaaaggtg ttcacactat aaaagcatat acgatgtgat 11580
ggtatttgat ggagcgtata ttgtatcagg tatttccgtc ggatacgaat tattcgtacg 11640
gccggaccgg tcccctaggc cggccaattc gagatcggcc gcggctgagt ggctccttca 11700
atcgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg 11760
tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt 11820
ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 11880
attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 11940
gtgcatgcca accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg 12000
gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca 12060
acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat 12120
cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga 12180
cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggagactgt 12240
catacgcgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 12300
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 12360
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 12420
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 12480
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 12540
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 12600
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 12660
ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 12720
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 12780
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 12840
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 12900
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 12960
tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 13020
tggtctgcag ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcgg 13080
tgcttttgcc gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaca 13140
cagaagccac tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca 13200
tcacccataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg 13260
aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg 13320
aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag 13380
aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga 13440
aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt 13500
gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta 13560
tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa 13620
ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg 13680
cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta 13740
tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag 13800
cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga 13860
ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt 13920
aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa 13980
catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc 14040
ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga 14100
acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa 14160
atattatatt ttactggatg aattgtttta gtacctagat gtggcgcaac gatgccggcg 14220
acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc 14280
acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca 14340
agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt 14400
atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt 14460
gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca 14520
ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca 14580
ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta 14640
cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg 14700
ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga 14760
ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg 14820
caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca 14880
aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc 14940
caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg 15000
cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg 15060
tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc 15120
gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct 15180
ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga 15240
cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc 15300
gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg 15360
aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca 15420
agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc 15480
cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt 15540
ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg 15600
cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg 15660
ctgggggttc agcagccagc gctttactgg catttcagga acaagcgggc actgctcgac 15720
gcacttgctt cgctcagtat cgctcgggac gcacggcgcg ctctacgaac tgccgataaa 15780
cagaggatta aaattgacaa ttcaatggca aggactgcca gcgctgccat ttttggggtg 15840
aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc 15900
cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc 15960
gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg 16020
ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga 16080
cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt 16140
caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc 16200
acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc 16260
gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc 16320
tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc 16380
agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca 16440
cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc 16500
gagggcaacc agcccggtga gcgtcgcaaa ggcgctcggt cttgccttgc tcgtcgagat 16560
ctggggtcga tcagccgggg atgcatcagg ccgacagtcg gaacttcggg tccccgacct 16620
gtaccattcg gtgagcaatg gataggggag ttgatatcgt caacgttcac ttctaaagaa 16680
atagcgccac tcagcttcct cagcggcttt atccagcgat ttcctattat gtcggcatag 16740
ttctcaagat cgacagcctg tcacggttaa gcgagaaatg aataagaagg ctgataattc 16800
ggatctctgc gagggagatg atatttgatc acaggcagca acgctctgtc atcgttacaa 16860
tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt 16920
cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg 16980
acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg 17040
tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt 17100
agacaactta ataacacatt gcggacgttt ttaatgtact ggggtggttt ttcttttcac 17160
cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa 17220
gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttccgaaatc 17280
ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt 17340
tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 17400
tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg 17460
tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 17520
aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgccattcag 17580
gctgcgcaac tgttgggaag gg 17602
<210> 24
<211> 14220
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd-HZE3
<400> 24
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctagca ccaaaggtcc atcggtcttt ccactggcac 3840
cttcttccaa gagtacttct ggaggcacag ctgcactggg ttgtcttgtc aaggactact 3900
ttccagaacc tgttacggtt tcgtggaact caggtgctct gaccagtgga gtgcacacct 3960
ttccagctgt tcttcagtcc tcaggattgt attctcttag cagtgttgtg actgttccat 4020
cctcaagctt gggcactcag acctacatct gcaatgtgaa tcacaaaccc agcaacacca 4080
aggttgacaa gaaagttgag cccaagtctt gtgacaagac tcatacgtgt ccaccgtgcc 4140
cagcacctga acttcttgga ggaccgtcag tcttcttgtt tcctccaaag cctaaggata 4200
ccttgatgat ctccaggact cctgaagtca catgtgtagt tgtggatgtg agccatgaag 4260
atcctgaggt gaagttcaac tggtatgtgg atggtgtgga agtgcacaat gccaagacaa 4320
agccgagaga ggaacagtac aacagcacgt acagggttgt ctcagttctc actgttctcc 4380
atcaagattg gttgaatggc aaagagtaca agtgcaaggt ctccaacaaa gccctcccag 4440
cccccattga gaagaccatt tccaaagcga aagggcaacc ccgtgaacca caagtgtaca 4500
cacttcctcc atctcgcgat gaactgacca agaaccaggt cagcttgact tgcctggtga 4560
aaggcttcta tccctctgac atagctgtag agtgggagag caatgggcaa ccggagaaca 4620
actacaagac tacacctccc gttctcgatt ctgacggctc cttcttcctc tacagcaagc 4680
tcacagtgga caagagcagg tggcaacaag ggaatgtctt ctcatgctcc gtgatgcatg 4740
aggctcttca caatcactac acacagaaga gtctctcctt gtctccgggt aaaggaggtg 4800
gcggatcagg tggaggcggt tcaggcggag gtggatccaa gggcgtgtca tactccttgt 4860
gtaccgctgc cttcacattc accaagatcc cggctgaaac actccacgga accgttaccg 4920
tggaggtcca atacgccggt acagatggac cttgcaaggt tccagctcag atggcggtgg 4980
acatgcaaac tcttacccca gttggaaggt tgattaccgc taaccccgtt atcactgaaa 5040
gcactgagaa ctctaagatg atgttggaac ttgatccacc attcggtgac tcttacattg 5100
tcattggtgt gggagagaag aagatcaccc accactggca caggagtggt agcacttaag 5160
agctcgaagt gacatcacaa agttgaaggt aataaagcca aattaattaa gacattttca 5220
taatgatgtc aagaatgcaa agcaaattgc ataactgcct ttatgcaaaa cattaatata 5280
atataaatta taaagaactg cgctctctgc ttcttatttt cttagcttca tttattagtc 5340
actagctgtt cagaattttc agtatctttt gatattacta agaacctaat cacacaatgt 5400
atattcttat gcaggaaaag cagaatgctg agctaaaaga aaggcttttt ccattttcga 5460
gagacaatga gaaaagaaga agaagaagaa gaagaagaag aagaagaaaa gagtaaataa 5520
taaagcccca caggaggcga agttcttgta gctccatgtt atctaagtta ttgatattgt 5580
ttgccctata ttttatttct gtcattgtgt atgttttgtt cagtttcgat ctccttgcaa 5640
aatgcagaga ttatgagatg aataaactaa gttatattat tatacgtgtt aatattctcc 5700
tcctctctct agctagcctt ttgttttctc tttttcttat ttgattttct ttaaatcaat 5760
ccattttagg agagggccag ggagtgatcc agcaaaacat gaagattaga agaaacttcc 5820
ctcttttttt tcctgaaaac aatttaacgt cgagatttat ctctttttgt aatggaatca 5880
tttctacagt tatgacgaat tgtacatcaa cgaaaaatta gtcaaacgac taaaataaat 5940
aaatatcatg tgttattaag aaaattctcc tataagaata ttttaataga tcatatgttt 6000
gtaaaaaaaa ttaattttta ctaacacata tatttactta tcaaaaattt gacaaagtaa 6060
gattaaaata atattcatct aacaaaaaaa aaaccagaaa atgctgaaaa cccggcaaaa 6120
ccgaaccaat ccaaaccgat atagttggtt tggtttgatt ttgatataaa ccgaaccaac 6180
tcggtccatt tgcaccccta atcataatag ctttaatatt tcaagatatt attaagttaa 6240
cgttgtcaat atcctggaaa ttttgcaaaa tgaatcaagc ctatatggct gtaatatgaa 6300
tttaaaagca gctcgatgtg gtggtaatat gtaatttact tgattctaaa aaaatatccc 6360
aagtattaat aatttctgct aggaagaagg ttagctacga tttacagcaa agccagaata 6420
caaagaacca taaagtgatt gaagctcgaa atatacgaag gaacaaatat ttttaaaaaa 6480
atacgcaatg acttggaaca aaagaaagtg atatattttt tgttcttaaa caagcatccc 6540
ctctaaagaa tggcagtttt cctttgcatg taactattat gctcccttcg ttacaaaaat 6600
tttggactac tattgggaac ttcttctgaa aatagtggta ccgagtgtac ttcaagtcag 6660
ttggaaatca ataaaatgat tattttatga atatatttca ttgtgcaagt agatagaaat 6720
tacatatgtt acataacaca cgaaataaac aaaaaaacac aatccaaaac aaacacccca 6780
aacaaaataa cactatatat atcctcgtat gaggagaggc acgttcagtg actcgacgat 6840
tcccgagcaa aaaaagtctc cccgtcacac atatagtggg tgacgcaatt atcttcaaag 6900
taatccttct gttgacttgt cattgataac atccagtctt cgtcaggatt gcaaagaatt 6960
atagaaggga tcccaccttt tattttcttc ttttttccat atttagggtt gacagtgaaa 7020
tcagactggc aacctattaa ttgcttccac aatgggacga acttgaaggg gatgtcgtcg 7080
atgatattat aggtggcgtg ttcatcgtag ttggtgaagt cgatggtccc gttccagtag 7140
ttgtgtcgcc cgagacttct agcccaggtg gtctttccgg tacgagttgg tccgcagatg 7200
tagaggctgg ggtgtctgac cccagtcctt ccctcatcct ggttagatcg gccatccact 7260
caaggtcaga ttgtgcttga tcgtaggaga caggatgtat gaaagtgtag gcatcgatgc 7320
ttacatgata taggtgcgtc tctctccagt tgtgcagatc ttcgtggcag cggagatctg 7380
attctgtgaa gggcgacacg tactgctcag gttgtggagg aaataatttg ttggctgaat 7440
attccagcca ttgaagcttt gttgcccatt catgagggaa ctcttctttg atcatgtcaa 7500
gatactcctc cttagacgtt gcagtctgga taatagttcg ccatcgtgcg tcagatttgc 7560
gaggagacac cttatgatct cggaaatctc ctctggtttt aatatctccg tcctttgata 7620
tgtaatcaag gacttgttta gagtttctag ctggctggat attagggtga tttccttcaa 7680
aatcgaaaaa agaaggatcc ctaatacaag gttttttatc aagctggata agagcatgat 7740
agtgggtagt gccatcttga tgaagctcag aagcaacacc aaggaagaaa ataagaaaag 7800
gtgtgagttt ctcccagaga aactggaata aatcatctct ttgagatgag cacttggggt 7860
aggtaaggaa aacatattta gattggagtc tgaagttctt gctagcagaa ggcatgttgt 7920
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 7980
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 8040
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 8100
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 8160
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 8220
gtatcaggta tttccgtcgg atacgaatta ttcgtacggc cggaccggtc ccctaggccg 8280
gccaattcga gatcggccgc ggctgagtgg ctccttcaat cgttgcggtt ctgtcagttc 8340
caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt 8400
ctccgctcat gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag 8460
gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa 8520
aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 8580
cccagatctg gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga 8640
cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat 8700
gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac 8760
cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag 8820
gggtatgttg ggtttcacgt ctggcctccg gagactgtca tacgcgtaaa aaggccgcgt 8880
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 8940
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 9000
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 9060
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 9120
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 9180
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 9240
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 9300
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 9360
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 9420
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 9480
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 9540
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 9600
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgcagtt gccatgtttt 9660
acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac 9720
cccgtcagta gctgaacagg agggacagct gatagacaca gaagccactg gagcacctca 9780
aaaacaccat catacactaa atcagtaagt tggcagcatc acccataatt gtggtttcaa 9840
aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt 9900
tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata 9960
attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct 10020
aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat 10080
acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat 10140
ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac 10200
atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat 10260
gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat 10320
gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt 10380
cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa 10440
ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac 10500
actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag 10560
gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa 10620
gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc 10680
ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt 10740
gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa 10800
ttgttttagt acctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt 10860
cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg 10920
gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac 10980
ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg 11040
cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg 11100
agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg 11160
gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga 11220
aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag 11280
cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg 11340
tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat 11400
gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa 11460
gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt 11520
cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc 11580
cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt 11640
ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga 11700
cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat 11760
caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta 11820
cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga 11880
ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg 11940
caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg 12000
cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg 12060
gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg 12120
cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc 12180
ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt 12240
gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc 12300
tttactggca tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg 12360
ctcgggacgc acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt 12420
caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc 12480
gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg 12540
gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg 12600
tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg 12660
cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg 12720
tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg 12780
tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac 12840
atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag 12900
ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag 12960
tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg 13020
aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc 13080
gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc 13140
gtcgcaaagg cgctcggtct tgccttgctc gtcgagatct ggggtcgatc agccggggat 13200
gcatcaggcc gacagtcgga acttcgggtc cccgacctgt accattcggt gagcaatgga 13260
taggggagtt gatatcgtca acgttcactt ctaaagaaat agcgccactc agcttcctca 13320
gcggctttat ccagcgattt cctattatgt cggcatagtt ctcaagatcg acagcctgtc 13380
acggttaagc gagaaatgaa taagaaggct gataattcgg atctctgcga gggagatgat 13440
atttgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag 13500
atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca 13560
tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc 13620
tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg 13680
gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc 13740
ggacgttttt aatgtactgg ggtggttttt cttttcacca gtgagacggg caacagctga 13800
ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 13860
agcaggcgaa aatcctgttt gatggtggtt ccgaaatcgg caaaatccct tataaatcaa 13920
aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 13980
agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 14040
gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 14100
accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 14160
aggaagggaa gaaagcgaaa ggagcgggcg ccattcaggc tgcgcaactg ttgggaaggg 14220
<210> 25
<211> 14232
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd-ZE3H
<400> 25
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttctcga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctagcacc aaaggtccat 4200
cggtctttcc actggcacct tcttccaaga gtacttctgg aggcacagct gcactgggtt 4260
gtcttgtcaa ggactacttt ccagaacctg ttacggtttc gtggaactca ggtgctctga 4320
ccagtggagt gcacaccttt ccagctgttc ttcagtcctc aggattgtat tctcttagca 4380
gtgttgtgac tgttccatcc tcaagcttgg gcactcagac ctacatctgc aatgtgaatc 4440
acaaacccag caacaccaag gttgacaaga aagttgagcc caagtcttgt gacaagactc 4500
atacgtgtcc accgtgccca gcacctgaac ttcttggagg accgtcagtc ttcttgtttc 4560
ctccaaagcc taaggatacc ttgatgatct ccaggactcc tgaagtcaca tgtgtagttg 4620
tggatgtgag ccatgaagat cctgaggtga agttcaactg gtatgtggat ggtgtggaag 4680
tgcacaatgc caagacaaag ccgagagagg aacagtacaa cagcacgtac agggttgtct 4740
cagttctcac tgttctccat caagattggt tgaatggcaa agagtacaag tgcaaggtct 4800
ccaacaaagc cctcccagcc cccattgaga agaccatttc caaagcgaaa gggcaacccc 4860
gtgaaccaca agtgtacaca cttcctccat ctcgcgatga actgaccaag aaccaggtca 4920
gcttgacttg cctggtgaaa ggcttctatc cctctgacat agctgtagag tgggagagca 4980
atgggcaacc ggagaacaac tacaagacta cacctcccgt tctcgattct gacggctcct 5040
tcttcctcta cagcaagctc acagtggaca agagcaggtg gcaacaaggg aatgtcttct 5100
catgctccgt gatgcatgag gctcttcaca atcactacac acagaagagt ctctccttgt 5160
ctccgggtaa agagctcgaa gtgacatcac aaagttgaag gtaataaagc caaattaatt 5220
aagacatttt cataatgatg tcaagaatgc aaagcaaatt gcataactgc ctttatgcaa 5280
aacattaata taatataaat tataaagaac tgcgctctct gcttcttatt ttcttagctt 5340
catttattag tcactagctg ttcagaattt tcagtatctt ttgatattac taagaaccta 5400
atcacacaat gtatattctt atgcaggaaa agcagaatgc tgagctaaaa gaaaggcttt 5460
ttccattttc gagagacaat gagaaaagaa gaagaagaag aagaagaaga agaagaagaa 5520
aagagtaaat aataaagccc cacaggaggc gaagttcttg tagctccatg ttatctaagt 5580
tattgatatt gtttgcccta tattttattt ctgtcattgt gtatgttttg ttcagtttcg 5640
atctccttgc aaaatgcaga gattatgaga tgaataaact aagttatatt attatacgtg 5700
ttaatattct cctcctctct ctagctagcc ttttgttttc tctttttctt atttgatttt 5760
ctttaaatca atccatttta ggagagggcc agggagtgat ccagcaaaac atgaagatta 5820
gaagaaactt ccctcttttt tttcctgaaa acaatttaac gtcgagattt atctcttttt 5880
gtaatggaat catttctaca gttatgacga attgtacatc aacgaaaaat tagtcaaacg 5940
actaaaataa ataaatatca tgtgttatta agaaaattct cctataagaa tattttaata 6000
gatcatatgt ttgtaaaaaa aattaatttt tactaacaca tatatttact tatcaaaaat 6060
ttgacaaagt aagattaaaa taatattcat ctaacaaaaa aaaaaccaga aaatgctgaa 6120
aacccggcaa aaccgaacca atccaaaccg atatagttgg tttggtttga ttttgatata 6180
aaccgaacca actcggtcca tttgcacccc taatcataat agctttaata tttcaagata 6240
ttattaagtt aacgttgtca atatcctgga aattttgcaa aatgaatcaa gcctatatgg 6300
ctgtaatatg aatttaaaag cagctcgatg tggtggtaat atgtaattta cttgattcta 6360
aaaaaatatc ccaagtatta ataatttctg ctaggaagaa ggttagctac gatttacagc 6420
aaagccagaa tacaaagaac cataaagtga ttgaagctcg aaatatacga aggaacaaat 6480
atttttaaaa aaatacgcaa tgacttggaa caaaagaaag tgatatattt tttgttctta 6540
aacaagcatc ccctctaaag aatggcagtt ttcctttgca tgtaactatt atgctccctt 6600
cgttacaaaa attttggact actattggga acttcttctg aaaatagtgg taccgagtgt 6660
acttcaagtc agttggaaat caataaaatg attattttat gaatatattt cattgtgcaa 6720
gtagatagaa attacatatg ttacataaca cacgaaataa acaaaaaaac acaatccaaa 6780
acaaacaccc caaacaaaat aacactatat atatcctcgt atgaggagag gcacgttcag 6840
tgactcgacg attcccgagc aaaaaaagtc tccccgtcac acatatagtg ggtgacgcaa 6900
ttatcttcaa agtaatcctt ctgttgactt gtcattgata acatccagtc ttcgtcagga 6960
ttgcaaagaa ttatagaagg gatcccacct tttattttct tcttttttcc atatttaggg 7020
ttgacagtga aatcagactg gcaacctatt aattgcttcc acaatgggac gaacttgaag 7080
gggatgtcgt cgatgatatt ataggtggcg tgttcatcgt agttggtgaa gtcgatggtc 7140
ccgttccagt agttgtgtcg cccgagactt ctagcccagg tggtctttcc ggtacgagtt 7200
ggtccgcaga tgtagaggct ggggtgtctg accccagtcc ttccctcatc ctggttagat 7260
cggccatcca ctcaaggtca gattgtgctt gatcgtagga gacaggatgt atgaaagtgt 7320
aggcatcgat gcttacatga tataggtgcg tctctctcca gttgtgcaga tcttcgtggc 7380
agcggagatc tgattctgtg aagggcgaca cgtactgctc aggttgtgga ggaaataatt 7440
tgttggctga atattccagc cattgaagct ttgttgccca ttcatgaggg aactcttctt 7500
tgatcatgtc aagatactcc tccttagacg ttgcagtctg gataatagtt cgccatcgtg 7560
cgtcagattt gcgaggagac accttatgat ctcggaaatc tcctctggtt ttaatatctc 7620
cgtcctttga tatgtaatca aggacttgtt tagagtttct agctggctgg atattagggt 7680
gatttccttc aaaatcgaaa aaagaaggat ccctaataca aggtttttta tcaagctgga 7740
taagagcatg atagtgggta gtgccatctt gatgaagctc agaagcaaca ccaaggaaga 7800
aaataagaaa aggtgtgagt ttctcccaga gaaactggaa taaatcatct ctttgagatg 7860
agcacttggg gtaggtaagg aaaacatatt tagattggag tctgaagttc ttgctagcag 7920
aaggcatgtt gttgtgactc cgaggggttg cctcaaactc tatcttataa ccggcgtgga 7980
ggcatggagg caagggcatt ttggtaattt aagtagttag tggaaaatga cgtcatttac 8040
ttaaagacga agtcttgcga caaggggggc ccacgccgaa ttttaatatt accggcgtgg 8100
ccccacctta tcgcgagtgc tttagcacga gcggtccaga tttaaagtag aaaagttccc 8160
gcccactagg gttaaaggtg ttcacactat aaaagcatat acgatgtgat ggtatttgat 8220
ggagcgtata ttgtatcagg tatttccgtc ggatacgaat tattcgtacg gccggaccgg 8280
tcccctaggc cggccaattc gagatcggcc gcggctgagt ggctccttca atcgttgcgg 8340
ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg 8400
actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc 8460
agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 8520
tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 8580
accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc 8640
gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag 8700
cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag 8760
gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag 8820
aattacgatc aggggtatgt tgggtttcac gtctggcctc cggagactgt catacgcgta 8880
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8940
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 9000
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 9060
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 9120
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 9180
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 9240
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 9300
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 9360
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9420
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9480
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9540
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9600
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgcag 9660
ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcgg tgcttttgcc 9720
gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaca cagaagccac 9780
tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccataa 9840
ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt 9900
tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt 9960
cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa 10020
taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc 10080
tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat 10140
gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa 10200
cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac 10260
tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc 10320
tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc 10380
atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc 10440
cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac 10500
tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa 10560
aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg 10620
aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg 10680
tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc 10740
gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt 10800
ttactggatg aattgtttta gtacctagat gtggcgcaac gatgccggcg acaagcagga 10860
gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta 10920
tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa 10980
ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac 11040
caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc 11100
aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact 11160
gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg 11220
tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat 11280
cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga 11340
gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac 11400
gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt 11460
cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca 11520
gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac 11580
ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa 11640
caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg 11700
ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat 11760
cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa 11820
tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg 11880
cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt 11940
cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt 12000
gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc 12060
gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct 12120
ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca 12180
ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa 12240
tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc 12300
agcagccagc gctttactgg catttcagga acaagcgggc actgctcgac gcacttgctt 12360
cgctcagtat cgctcgggac gcacggcgcg ctctacgaac tgccgataaa cagaggatta 12420
aaattgacaa ttcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg 12480
cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 12540
cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 12600
ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 12660
ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 12720
aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 12780
gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 12840
aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 12900
gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 12960
gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 13020
aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 13080
ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 13140
agcccggtga gcgtcgcaaa ggcgctcggt cttgccttgc tcgtcgagat ctggggtcga 13200
tcagccgggg atgcatcagg ccgacagtcg gaacttcggg tccccgacct gtaccattcg 13260
gtgagcaatg gataggggag ttgatatcgt caacgttcac ttctaaagaa atagcgccac 13320
tcagcttcct cagcggcttt atccagcgat ttcctattat gtcggcatag ttctcaagat 13380
cgacagcctg tcacggttaa gcgagaaatg aataagaagg ctgataattc ggatctctgc 13440
gagggagatg atatttgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct 13500
accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata 13560
gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc 13620
ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct 13680
gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta 13740
ataacacatt gcggacgttt ttaatgtact ggggtggttt ttcttttcac cagtgagacg 13800
ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg 13860
ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttccgaaatc ggcaaaatcc 13920
cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga 13980
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 14040
atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag 14100
cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 14160
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgccattcag gctgcgcaac 14220
tgttgggaag gg 14232
<210> 26
<211> 14235
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd-ZE3Hx
<400> 26
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttcttga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctagcacc aaaggtccat 4200
cggtctttcc actggcacct tcttccaaga gtacttctgg aggcacagct gcactgggtt 4260
gtcttgtcaa ggactacttt ccagaacctg ttacggtttc gtggaactca ggtgctctga 4320
ccagtggagt gcacaccttt ccagctgttc ttcagtcctc aggattgtat tctcttagca 4380
gtgttgtgac tgttccatcc tcaagcttgg gcactcagac ctacatctgc aatgtgaatc 4440
acaaacccag caacaccaag gttgacaaga aagttgagcc caagtcttgt gacaagactc 4500
atacgtgtcc accgtgccca gcacctgaac ttcttggagg accgtcagtc ttcttgtttc 4560
ctccaaagcc taaggatacc ttgatgatct ccaggactcc tgaagtcaca tgtgtagttg 4620
tggatgtgag ccatgaagat cctgaggtga agttcaactg gtatgtggat ggtgtggaag 4680
tgcacaatgc caagacaaag ccgagagagg aacagtacaa cagcacgtac agggttgtct 4740
cagttctcac tgttctccat caagattggt tgaatggcaa agagtacaag tgcaaggtct 4800
ccaacaaagc cctcccagct cctattgaga agaccatctc taaagccaag ggtcaaccgc 4860
gtcgtccaca agtgtacaca cttcctccat ctcgcgacga actcaccaag aatcaagtca 4920
gccttacttg tctcgttaag ggcttctatc cctccgacat cgctgttgag tgggagtcaa 4980
acggacaacc ggagaacaat tacaagacta cacctccagt tttggattcc gatggctcct 5040
tcttcctcta cagcaagctc acagtggaca aatctaggtg gcaacaaggc aacgtcttct 5100
catgctccgt gatgcatgga gcccttcaca atcactacac acagaagtac ctctccttgt 5160
ctccgggaaa gtgagagctc gaagtgacat cacaaagttg aaggtaataa agccaaatta 5220
attaagacat tttcataatg atgtcaagaa tgcaaagcaa attgcataac tgcctttatg 5280
caaaacatta atataatata aattataaag aactgcgctc tctgcttctt attttcttag 5340
cttcatttat tagtcactag ctgttcagaa ttttcagtat cttttgatat tactaagaac 5400
ctaatcacac aatgtatatt cttatgcagg aaaagcagaa tgctgagcta aaagaaaggc 5460
tttttccatt ttcgagagac aatgagaaaa gaagaagaag aagaagaaga agaagaagaa 5520
gaaaagagta aataataaag ccccacagga ggcgaagttc ttgtagctcc atgttatcta 5580
agttattgat attgtttgcc ctatatttta tttctgtcat tgtgtatgtt ttgttcagtt 5640
tcgatctcct tgcaaaatgc agagattatg agatgaataa actaagttat attattatac 5700
gtgttaatat tctcctcctc tctctagcta gccttttgtt ttctcttttt cttatttgat 5760
tttctttaaa tcaatccatt ttaggagagg gccagggagt gatccagcaa aacatgaaga 5820
ttagaagaaa cttccctctt ttttttcctg aaaacaattt aacgtcgaga tttatctctt 5880
tttgtaatgg aatcatttct acagttatga cgaattgtac atcaacgaaa aattagtcaa 5940
acgactaaaa taaataaata tcatgtgtta ttaagaaaat tctcctataa gaatatttta 6000
atagatcata tgtttgtaaa aaaaattaat ttttactaac acatatattt acttatcaaa 6060
aatttgacaa agtaagatta aaataatatt catctaacaa aaaaaaaacc agaaaatgct 6120
gaaaacccgg caaaaccgaa ccaatccaaa ccgatatagt tggtttggtt tgattttgat 6180
ataaaccgaa ccaactcggt ccatttgcac ccctaatcat aatagcttta atatttcaag 6240
atattattaa gttaacgttg tcaatatcct ggaaattttg caaaatgaat caagcctata 6300
tggctgtaat atgaatttaa aagcagctcg atgtggtggt aatatgtaat ttacttgatt 6360
ctaaaaaaat atcccaagta ttaataattt ctgctaggaa gaaggttagc tacgatttac 6420
agcaaagcca gaatacaaag aaccataaag tgattgaagc tcgaaatata cgaaggaaca 6480
aatattttta aaaaaatacg caatgacttg gaacaaaaga aagtgatata ttttttgttc 6540
ttaaacaagc atcccctcta aagaatggca gttttccttt gcatgtaact attatgctcc 6600
cttcgttaca aaaattttgg actactattg ggaacttctt ctgaaaatag tggtaccgag 6660
tgtacttcaa gtcagttgga aatcaataaa atgattattt tatgaatata tttcattgtg 6720
caagtagata gaaattacat atgttacata acacacgaaa taaacaaaaa aacacaatcc 6780
aaaacaaaca ccccaaacaa aataacacta tatatatcct cgtatgagga gaggcacgtt 6840
cagtgactcg acgattcccg agcaaaaaaa gtctccccgt cacacatata gtgggtgacg 6900
caattatctt caaagtaatc cttctgttga cttgtcattg ataacatcca gtcttcgtca 6960
ggattgcaaa gaattataga agggatccca ccttttattt tcttcttttt tccatattta 7020
gggttgacag tgaaatcaga ctggcaacct attaattgct tccacaatgg gacgaacttg 7080
aaggggatgt cgtcgatgat attataggtg gcgtgttcat cgtagttggt gaagtcgatg 7140
gtcccgttcc agtagttgtg tcgcccgaga cttctagccc aggtggtctt tccggtacga 7200
gttggtccgc agatgtagag gctggggtgt ctgaccccag tccttccctc atcctggtta 7260
gatcggccat ccactcaagg tcagattgtg cttgatcgta ggagacagga tgtatgaaag 7320
tgtaggcatc gatgcttaca tgatataggt gcgtctctct ccagttgtgc agatcttcgt 7380
ggcagcggag atctgattct gtgaagggcg acacgtactg ctcaggttgt ggaggaaata 7440
atttgttggc tgaatattcc agccattgaa gctttgttgc ccattcatga gggaactctt 7500
ctttgatcat gtcaagatac tcctccttag acgttgcagt ctggataata gttcgccatc 7560
gtgcgtcaga tttgcgagga gacaccttat gatctcggaa atctcctctg gttttaatat 7620
ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt tctagctggc tggatattag 7680
ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat acaaggtttt ttatcaagct 7740
ggataagagc atgatagtgg gtagtgccat cttgatgaag ctcagaagca acaccaagga 7800
agaaaataag aaaaggtgtg agtttctccc agagaaactg gaataaatca tctctttgag 7860
atgagcactt ggggtaggta aggaaaacat atttagattg gagtctgaag ttcttgctag 7920
cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt 7980
ggaggcatgg aggcaagggc attttggtaa tttaagtagt tagtggaaaa tgacgtcatt 8040
tacttaaaga cgaagtcttg cgacaagggg ggcccacgcc gaattttaat attaccggcg 8100
tggccccacc ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaagtt 8160
cccgcccact agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt 8220
gatggagcgt atattgtatc aggtatttcc gtcggatacg aattattcgt acggccggac 8280
cggtccccta ggccggccaa ttcgagatcg gccgcggctg agtggctcct tcaatcgttg 8340
cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac 8400
gtgactccct taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact 8460
atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 8520
taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg 8580
ccaaccacag ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg 8640
cccgaaacga tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata 8700
cagcgccagc agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag 8760
gaggcccggc agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct 8820
cagaattacg atcaggggta tgttgggttt cacgtctggc ctccggagac tgtcatacgc 8880
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8940
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 9000
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 9060
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 9120
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 9180
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 9240
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 9300
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 9360
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9420
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9480
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9540
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 9600
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 9660
cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt 9720
gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag acacagaagc 9780
cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccca 9840
taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa 9900
ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga 9960
gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg 10020
aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac 10080
cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa 10140
aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg 10200
gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg 10260
cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt 10320
tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag 10380
tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac 10440
agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa 10500
aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg 10560
gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt 10620
gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag 10680
tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat 10740
gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat 10800
attttactgg atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca 10860
ggagcgcacc gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa 10920
gtatttgggc aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga 10980
gaaggacggc cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga 11040
caccaaggca ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg 11100
ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga 11160
actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat 11220
gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa 11280
gatcgagcgc gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt 11340
ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga 11400
cacgcgagga actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca 11460
ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat 11520
gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga 11580
cacggcccgc tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca 11640
aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct 11700
gcgggccgac gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc 11760
tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat 11820
caatggccgg tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat 11880
gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg 11940
cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt 12000
cgtgctgttt gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc 12060
gccgacggcc cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa 12120
gctggaaacc ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga 12180
gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt 12240
caatgatgac ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg 12300
ttcagcagcc agcgctttac tggcatttca ggaacaagcg ggcactgctc gacgcacttg 12360
cttcgctcag tatcgctcgg gacgcacggc gcgctctacg aactgccgat aaacagagga 12420
ttaaaattga caattcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt 12480
tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg 12540
gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc 12600
tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg 12660
tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc 12720
tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat 12780
cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc 12840
cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt 12900
tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac 12960
gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg 13020
gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc 13080
gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca 13140
accagcccgg tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcga gatctggggt 13200
cgatcagccg gggatgcatc aggccgacag tcggaacttc gggtccccga cctgtaccat 13260
tcggtgagca atggataggg gagttgatat cgtcaacgtt cacttctaaa gaaatagcgc 13320
cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca tagttctcaa 13380
gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa ttcggatctc 13440
tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta caatcaacat 13500
gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga 13560
atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt 13620
cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga 13680
gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac 13740
ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag 13800
acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc 13860
acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa atcggcaaaa 13920
tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca 13980
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 14040
gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta 14100
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 14160
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt caggctgcgc 14220
aactgttggg aaggg 14235
<210> 27
<211> 14655
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd-HVLZe
<400> 27
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctacta gtggtggatc aggaggttct ggtggttctg 3840
gaggttcagg atccgatgtt ttgatgactc aaagccctct ctcacttcct gtgactcttg 3900
gacagcccgc atccatatct tgcagatcta gtcagagtat tgttcatagt aacggcaaca 3960
cctacttgga atggtatctg cagaaaccag gccagtctcc aaagcttctg atctacaagg 4020
cttccaatcg tttctctggt gtcccagaca ggtttagtgg cagtggatca gggactgact 4080
tcacattgaa gatcagcaga gttgaggctg aagatgcggg agtgtactat tgtcttcaag 4140
gttcacatgt tccgtcaacg tttggaggtg ggaccaaagt ggagatcaag gctagcacca 4200
aaggtccatc ggtctttcca ctggcacctt cttccaagag tacttctgga ggcacagctg 4260
cactgggttg tcttgtcaag gactactttc cagaacctgt tacggtttcg tggaactcag 4320
gtgctctgac cagtggagtg cacacctttc cagctgttct tcagtcctca ggattgtatt 4380
ctcttagcag tgttgtgact gttccatcct caagcttggg cactcagacc tacatctgca 4440
atgtgaatca caaacccagc aacaccaagg ttgacaagaa agttgagccc aagtcttgtg 4500
acaagactca tacgtgtcca ccgtgcccag cacctgaact tcttggagga ccgtcagtct 4560
tcttgtttcc tccaaagcct aaggatacct tgatgatctc caggactcct gaagtcacat 4620
gtgtagttgt ggatgtgagc catgaagatc ctgaggtgaa gttcaactgg tatgtggatg 4680
gtgtggaagt gcacaatgcc aagacaaagc cgagagagga acagtacaac agcacgtaca 4740
gggttgtctc agttctcact gttctccatc aagattggtt gaatggcaaa gagtacaagt 4800
gcaaggtctc caacaaagcc ctcccagccc ccattgagaa gaccatttcc aaagcgaaag 4860
ggcaaccccg tgaaccacaa gtgtacacac ttcctccatc tcgcgatgaa ctgaccaaga 4920
accaggtcag cttgacttgc ctggtgaaag gcttctatcc ctctgacata gctgtagagt 4980
gggagagcaa tgggcaaccg gagaacaact acaagactac acctcccgtt ctcgattctg 5040
acggctcctt cttcctctac agcaagctca cagtggacaa gagcaggtgg caacaaggga 5100
atgtcttctc atgctccgtg atgcatgagg ctcttcacaa tcactacaca cagaagagtc 5160
tctccttgtc tccgggtaaa ggaggtggcg gatcaggtgg aggcggttca ggcggaggtg 5220
gatccaaggg cgtgtcatac tccttgtgta ccgctgcctt cacattcacc aagatcccgg 5280
ctgaaacact ccacggaacc gttaccgtgg aggtccaata cgccggtaca gatggacctt 5340
gcaaggttcc agctcagatg gcggtggaca tgcaaactct taccccagtt ggaaggttga 5400
ttaccgctaa ccccgttatc actgaaagca ctgagaactc taagatgatg ttggaacttg 5460
atccaccatt cggtgactct tacattgtca ttggtgtggg agagaagaag atcacccacc 5520
actggcacag gagtggtagc actagtcata acactcctgt ttacaagctg gacatatctg 5580
aggcaactca ataagagctc gaagtgacat cacaaagttg aaggtaataa agccaaatta 5640
attaagacat tttcataatg atgtcaagaa tgcaaagcaa attgcataac tgcctttatg 5700
caaaacatta atataatata aattataaag aactgcgctc tctgcttctt attttcttag 5760
cttcatttat tagtcactag ctgttcagaa ttttcagtat cttttgatat tactaagaac 5820
ctaatcacac aatgtatatt cttatgcagg aaaagcagaa tgctgagcta aaagaaaggc 5880
tttttccatt ttcgagagac aatgagaaaa gaagaagaag aagaagaaga agaagaagaa 5940
gaaaagagta aataataaag ccccacagga ggcgaagttc ttgtagctcc atgttatcta 6000
agttattgat attgtttgcc ctatatttta tttctgtcat tgtgtatgtt ttgttcagtt 6060
tcgatctcct tgcaaaatgc agagattatg agatgaataa actaagttat attattatac 6120
gtgttaatat tctcctcctc tctctagcta gccttttgtt ttctcttttt cttatttgat 6180
tttctttaaa tcaatccatt ttaggagagg gccagggagt gatccagcaa aacatgaaga 6240
ttagaagaaa cttccctctt ttttttcctg aaaacaattt aacgtcgaga tttatctctt 6300
tttgtaatgg aatcatttct acagttatga cgaattgtac atcaacgaaa aattagtcaa 6360
acgactaaaa taaataaata tcatgtgtta ttaagaaaat tctcctataa gaatatttta 6420
atagatcata tgtttgtaaa aaaaattaat ttttactaac acatatattt acttatcaaa 6480
aatttgacaa agtaagatta aaataatatt catctaacaa aaaaaaaacc agaaaatgct 6540
gaaaacccgg caaaaccgaa ccaatccaaa ccgatatagt tggtttggtt tgattttgat 6600
ataaaccgaa ccaactcggt ccatttgcac ccctaatcat aatagcttta atatttcaag 6660
atattattaa gttaacgttg tcaatatcct ggaaattttg caaaatgaat caagcctata 6720
tggctgtaat atgaatttaa aagcagctcg atgtggtggt aatatgtaat ttacttgatt 6780
ctaaaaaaat atcccaagta ttaataattt ctgctaggaa gaaggttagc tacgatttac 6840
agcaaagcca gaatacaaag aaccataaag tgattgaagc tcgaaatata cgaaggaaca 6900
aatattttta aaaaaatacg caatgacttg gaacaaaaga aagtgatata ttttttgttc 6960
ttaaacaagc atcccctcta aagaatggca gttttccttt gcatgtaact attatgctcc 7020
cttcgttaca aaaattttgg actactattg ggaacttctt ctgaaaatag tggtaccgag 7080
tgtacttcaa gtcagttgga aatcaataaa atgattattt tatgaatata tttcattgtg 7140
caagtagata gaaattacat atgttacata acacacgaaa taaacaaaaa aacacaatcc 7200
aaaacaaaca ccccaaacaa aataacacta tatatatcct cgtatgagga gaggcacgtt 7260
cagtgactcg acgattcccg agcaaaaaaa gtctccccgt cacacatata gtgggtgacg 7320
caattatctt caaagtaatc cttctgttga cttgtcattg ataacatcca gtcttcgtca 7380
ggattgcaaa gaattataga agggatccca ccttttattt tcttcttttt tccatattta 7440
gggttgacag tgaaatcaga ctggcaacct attaattgct tccacaatgg gacgaacttg 7500
aaggggatgt cgtcgatgat attataggtg gcgtgttcat cgtagttggt gaagtcgatg 7560
gtcccgttcc agtagttgtg tcgcccgaga cttctagccc aggtggtctt tccggtacga 7620
gttggtccgc agatgtagag gctggggtgt ctgaccccag tccttccctc atcctggtta 7680
gatcggccat ccactcaagg tcagattgtg cttgatcgta ggagacagga tgtatgaaag 7740
tgtaggcatc gatgcttaca tgatataggt gcgtctctct ccagttgtgc agatcttcgt 7800
ggcagcggag atctgattct gtgaagggcg acacgtactg ctcaggttgt ggaggaaata 7860
atttgttggc tgaatattcc agccattgaa gctttgttgc ccattcatga gggaactctt 7920
ctttgatcat gtcaagatac tcctccttag acgttgcagt ctggataata gttcgccatc 7980
gtgcgtcaga tttgcgagga gacaccttat gatctcggaa atctcctctg gttttaatat 8040
ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt tctagctggc tggatattag 8100
ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat acaaggtttt ttatcaagct 8160
ggataagagc atgatagtgg gtagtgccat cttgatgaag ctcagaagca acaccaagga 8220
agaaaataag aaaaggtgtg agtttctccc agagaaactg gaataaatca tctctttgag 8280
atgagcactt ggggtaggta aggaaaacat atttagattg gagtctgaag ttcttgctag 8340
cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt 8400
ggaggcatgg aggcaagggc attttggtaa tttaagtagt tagtggaaaa tgacgtcatt 8460
tacttaaaga cgaagtcttg cgacaagggg ggcccacgcc gaattttaat attaccggcg 8520
tggccccacc ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaagtt 8580
cccgcccact agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt 8640
gatggagcgt atattgtatc aggtatttcc gtcggatacg aattattcgt acggccggac 8700
cggtccccta ggccggccaa ttcgagatcg gccgcggctg agtggctcct tcaatcgttg 8760
cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac 8820
gtgactccct taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact 8880
atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 8940
taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg 9000
ccaaccacag ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg 9060
cccgaaacga tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata 9120
cagcgccagc agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag 9180
gaggcccggc agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct 9240
cagaattacg atcaggggta tgttgggttt cacgtctggc ctccggagac tgtcatacgc 9300
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 9360
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 9420
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 9480
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 9540
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 9600
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 9660
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 9720
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 9780
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9840
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9900
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9960
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 10020
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 10080
cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt 10140
gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag acacagaagc 10200
cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccca 10260
taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa 10320
ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga 10380
gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg 10440
aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac 10500
cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa 10560
aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg 10620
gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg 10680
cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt 10740
tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag 10800
tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac 10860
agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa 10920
aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg 10980
gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt 11040
gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag 11100
tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat 11160
gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat 11220
attttactgg atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca 11280
ggagcgcacc gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa 11340
gtatttgggc aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga 11400
gaaggacggc cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga 11460
caccaaggca ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg 11520
ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga 11580
actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat 11640
gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa 11700
gatcgagcgc gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt 11760
ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga 11820
cacgcgagga actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca 11880
ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat 11940
gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga 12000
cacggcccgc tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca 12060
aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct 12120
gcgggccgac gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc 12180
tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat 12240
caatggccgg tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat 12300
gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg 12360
cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt 12420
cgtgctgttt gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc 12480
gccgacggcc cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa 12540
gctggaaacc ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga 12600
gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt 12660
caatgatgac ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg 12720
ttcagcagcc agcgctttac tggcatttca ggaacaagcg ggcactgctc gacgcacttg 12780
cttcgctcag tatcgctcgg gacgcacggc gcgctctacg aactgccgat aaacagagga 12840
ttaaaattga caattcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt 12900
tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg 12960
gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc 13020
tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg 13080
tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc 13140
tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat 13200
cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc 13260
cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt 13320
tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac 13380
gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg 13440
gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc 13500
gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca 13560
accagcccgg tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcga gatctggggt 13620
cgatcagccg gggatgcatc aggccgacag tcggaacttc gggtccccga cctgtaccat 13680
tcggtgagca atggataggg gagttgatat cgtcaacgtt cacttctaaa gaaatagcgc 13740
cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca tagttctcaa 13800
gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa ttcggatctc 13860
tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta caatcaacat 13920
gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga 13980
atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt 14040
cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga 14100
gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac 14160
ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag 14220
acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc 14280
acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa atcggcaaaa 14340
tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca 14400
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 14460
gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta 14520
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 14580
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt caggctgcgc 14640
aactgttggg aaggg 14655
<210> 28
<211> 14244
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd2-HVL-Hx
<400> 28
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctacta gtggtggatc aggaggttct ggtggttctg 3840
gaggttcagg atccgatgtt ttgatgactc aaagccctct ctcacttcct gtgactcttg 3900
gacagcccgc atccatatct tgcagatcta gtcagagtat tgttcatagt aacggcaaca 3960
cctacttgga atggtatctg cagaaaccag gccagtctcc aaagcttctg atctacaagg 4020
cttccaatcg tttctctggt gtcccagaca ggtttagtgg cagtggatca gggactgact 4080
tcacattgaa gatcagcaga gttgaggctg aagatgcggg agtgtactat tgtcttcaag 4140
gttcacatgt tccgtcaacg tttggaggtg ggaccaaagt ggagatcaag gctagcacca 4200
aaggtccatc ggtctttcca ctggcacctt cttccaagag tacttctgga ggcacagctg 4260
cactgggttg tcttgtcaag gactactttc cagaacctgt tacggtttcg tggaactcag 4320
gtgctctgac cagtggagtg cacacctttc cagctgttct tcagtcctca ggattgtatt 4380
ctcttagcag tgttgtgact gttccatcct caagcttggg cactcagacc tacatctgca 4440
atgtgaatca caaacccagc aacaccaagg ttgacaagaa agttgagccc aagtcttgtg 4500
acaagactca tacgtgtcca ccgtgcccag cacctgaact tcttggagga ccgtcagtct 4560
tcttgtttcc tccaaagcct aaggatacct tgatgatctc caggactcct gaagtcacat 4620
gtgtagttgt ggatgtgagc catgaagatc ctgaggtgaa gttcaactgg tatgtggatg 4680
gtgtggaagt gcacaatgcc aagacaaagc cgagagagga acagtacaac agcacgtaca 4740
gggttgtctc agttctcact gttctccatc aagattggtt gaatggcaaa gagtacaagt 4800
gcaaggtctc caacaaagcc ctcccagctc ctattgagaa gaccatctct aaagccaagg 4860
gtcaaccgcg tcgtccacaa gtgtacacac ttcctccatc tcgcgacgaa ctcaccaaga 4920
atcaagtcag ccttacttgt ctcgttaagg gcttctatcc ctccgacatc gctgttgagt 4980
gggagtcaaa cggacaaccg gagaacaatt acaagactac acctccagtt ttggattccg 5040
atggctcctt cttcctctac agcaagctca cagtggacaa atctaggtgg caacaaggca 5100
acgtcttctc atgctccgtg atgcatggag cccttcacaa tcactacaca cagaagtacc 5160
tctccttgtc tccgggaaag tgagagctcg aagtgacatc acaaagttga aggtaataaa 5220
gccaaattaa ttaagacatt ttcataatga tgtcaagaat gcaaagcaaa ttgcataact 5280
gcctttatgc aaaacattaa tataatataa attataaaga actgcgctct ctgcttctta 5340
ttttcttagc ttcatttatt agtcactagc tgttcagaat tttcagtatc ttttgatatt 5400
actaagaacc taatcacaca atgtatattc ttatgcagga aaagcagaat gctgagctaa 5460
aagaaaggct ttttccattt tcgagagaca atgagaaaag aagaagaaga agaagaagaa 5520
gaagaagaag aaaagagtaa ataataaagc cccacaggag gcgaagttct tgtagctcca 5580
tgttatctaa gttattgata ttgtttgccc tatattttat ttctgtcatt gtgtatgttt 5640
tgttcagttt cgatctcctt gcaaaatgca gagattatga gatgaataaa ctaagttata 5700
ttattatacg tgttaatatt ctcctcctct ctctagctag ccttttgttt tctctttttc 5760
ttatttgatt ttctttaaat caatccattt taggagaggg ccagggagtg atccagcaaa 5820
acatgaagat tagaagaaac ttccctcttt tttttcctga aaacaattta acgtcgagat 5880
ttatctcttt ttgtaatgga atcatttcta cagttatgac gaattgtaca tcaacgaaaa 5940
attagtcaaa cgactaaaat aaataaatat catgtgttat taagaaaatt ctcctataag 6000
aatattttaa tagatcatat gtttgtaaaa aaaattaatt tttactaaca catatattta 6060
cttatcaaaa atttgacaaa gtaagattaa aataatattc atctaacaaa aaaaaaacca 6120
gaaaatgctg aaaacccggc aaaaccgaac caatccaaac cgatatagtt ggtttggttt 6180
gattttgata taaaccgaac caactcggtc catttgcacc cctaatcata atagctttaa 6240
tatttcaaga tattattaag ttaacgttgt caatatcctg gaaattttgc aaaatgaatc 6300
aagcctatat ggctgtaata tgaatttaaa agcagctcga tgtggtggta atatgtaatt 6360
tacttgattc taaaaaaata tcccaagtat taataatttc tgctaggaag aaggttagct 6420
acgatttaca gcaaagccag aatacaaaga accataaagt gattgaagct cgaaatatac 6480
gaaggaacaa atatttttaa aaaaatacgc aatgacttgg aacaaaagaa agtgatatat 6540
tttttgttct taaacaagca tcccctctaa agaatggcag ttttcctttg catgtaacta 6600
ttatgctccc ttcgttacaa aaattttgga ctactattgg gaacttcttc tgaaaatagt 6660
ggtaccgagt gtacttcaag tcagttggaa atcaataaaa tgattatttt atgaatatat 6720
ttcattgtgc aagtagatag aaattacata tgttacataa cacacgaaat aaacaaaaaa 6780
acacaatcca aaacaaacac cccaaacaaa ataacactat atatatcctc gtatgaggag 6840
aggcacgttc agtgactcga cgattcccga gcaaaaaaag tctccccgtc acacatatag 6900
tgggtgacgc aattatcttc aaagtaatcc ttctgttgac ttgtcattga taacatccag 6960
tcttcgtcag gattgcaaag aattatagaa gggatcccac cttttatttt cttctttttt 7020
ccatatttag ggttgacagt gaaatcagac tggcaaccta ttaattgctt ccacaatggg 7080
acgaacttga aggggatgtc gtcgatgata ttataggtgg cgtgttcatc gtagttggtg 7140
aagtcgatgg tcccgttcca gtagttgtgt cgcccgagac ttctagccca ggtggtcttt 7200
ccggtacgag ttggtccgca gatgtagagg ctggggtgtc tgaccccagt ccttccctca 7260
tcctggttag atcggccatc cactcaaggt cagattgtgc ttgatcgtag gagacaggat 7320
gtatgaaagt gtaggcatcg atgcttacat gatataggtg cgtctctctc cagttgtgca 7380
gatcttcgtg gcagcggaga tctgattctg tgaagggcga cacgtactgc tcaggttgtg 7440
gaggaaataa tttgttggct gaatattcca gccattgaag ctttgttgcc cattcatgag 7500
ggaactcttc tttgatcatg tcaagatact cctccttaga cgttgcagtc tggataatag 7560
ttcgccatcg tgcgtcagat ttgcgaggag acaccttatg atctcggaaa tctcctctgg 7620
ttttaatatc tccgtccttt gatatgtaat caaggacttg tttagagttt ctagctggct 7680
ggatattagg gtgatttcct tcaaaatcga aaaaagaagg atccctaata caaggttttt 7740
tatcaagctg gataagagca tgatagtggg tagtgccatc ttgatgaagc tcagaagcaa 7800
caccaaggaa gaaaataaga aaaggtgtga gtttctccca gagaaactgg aataaatcat 7860
ctctttgaga tgagcacttg gggtaggtaa ggaaaacata tttagattgg agtctgaagt 7920
tcttgctagc agaaggcatg ttgttgtgac tccgaggggt tgcctcaaac tctatcttat 7980
aaccggcgtg gaggcatgga ggcaagggca ttttggtaat ttaagtagtt agtggaaaat 8040
gacgtcattt acttaaagac gaagtcttgc gacaaggggg gcccacgccg aattttaata 8100
ttaccggcgt ggccccacct tatcgcgagt gctttagcac gagcggtcca gatttaaagt 8160
agaaaagttc ccgcccacta gggttaaagg tgttcacact ataaaagcat atacgatgtg 8220
atggtatttg atggagcgta tattgtatca ggtatttccg tcggatacga attattcgta 8280
cggccggacc ggtcccctag gccggccaat tcgagatcgg ccgcggctga gtggctcctt 8340
caatcgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg 8400
ggtcataacg tgactccctt aattctccgc tcatgatcag attgtcgttt cccgccttca 8460
gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 8520
ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt 8580
atgtgcatgc caaccacagg gttccccaga tctggcgccg gccagcgaga cgagcaagat 8640
tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac 8700
caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag 8760
atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc 8820
gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc tccggagact 8880
gtcatacgcg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 8940
agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 9000
accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 9060
ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 9120
gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 9180
ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 9240
gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 9300
taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 9360
tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 9420
gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 9480
cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 9540
agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 9600
cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 9660
cttggtctgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc 9720
ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga 9780
cacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag 9840
catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt 9900
tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag 9960
tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa 10020
agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc 10080
gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg 10140
gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc 10200
tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca 10260
aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat 10320
ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg 10380
tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat 10440
agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg 10500
gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt 10560
ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc 10620
aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg 10680
gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa 10740
gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata 10800
aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg 10860
cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc 10920
ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac 10980
caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga 11040
ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc 11100
gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata 11160
caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg 11220
caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc 11280
tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc 11340
ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat 11400
gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct 11460
ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat 11520
caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat 11580
gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga 11640
ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg 11700
cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa 11760
gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg 11820
ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc 11880
gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca 11940
ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga 12000
ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg 12060
caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta 12120
cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa 12180
gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca 12240
cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc 12300
ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg 12360
acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata 12420
aacagaggat taaaattgac aattcaatgg caaggactgc cagcgctgcc atttttgggg 12480
tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg 12540
gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg 12600
gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca 12660
ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg 12720
gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt 12780
gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg 12840
gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt 12900
tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca 12960
tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg 13020
tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca 13080
cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg 13140
gcgagggcaa ccagcccggt gagcgtcgca aaggcgctcg gtcttgcctt gctcgtcgag 13200
atctggggtc gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac 13260
ctgtaccatt cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag 13320
aaatagcgcc actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat 13380
agttctcaag atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat 13440
tcggatctct gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac 13500
aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 13560
ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 13620
tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 13680
ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 13740
ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc 13800
accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc 13860
aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa 13920
tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag 13980
tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 14040
tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga 14100
ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 14160
gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc 14220
aggctgcgca actgttggga aggg 14244
<210> 29
<211> 14607
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd2-HVLZnt
<400> 29
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctacta gtggtggatc aggaggttct ggtggttctg 3840
gaggttcagg atccgatgtt ttgatgactc aaagccctct ctcacttcct gtgactcttg 3900
gacagcccgc atccatatct tgcagatcta gtcagagtat tgttcatagt aacggcaaca 3960
cctacttgga atggtatctg cagaaaccag gccagtctcc aaagcttctg atctacaagg 4020
cttccaatcg tttctctggt gtcccagaca ggtttagtgg cagtggatca gggactgact 4080
tcacattgaa gatcagcaga gttgaggctg aagatgcggg agtgtactat tgtcttcaag 4140
gttcacatgt tccgtcaacg tttggaggtg ggaccaaagt ggagatcaag gctagcacca 4200
aaggtccatc ggtctttcca ctggcacctt cttccaagag tacttctgga ggcacagctg 4260
cactgggttg tcttgtcaag gactactttc cagaacctgt tacggtttcg tggaactcag 4320
gtgctctgac cagtggagtg cacacctttc cagctgttct tcagtcctca ggattgtatt 4380
ctcttagcag tgttgtgact gttccatcct caagcttggg cactcagacc tacatctgca 4440
atgtgaatca caaacccagc aacaccaagg ttgacaagaa agttgagccc aagtcttgtg 4500
acaagactca tacgtgtcca ccgtgcccag cacctgaact tcttggagga ccgtcagtct 4560
tcttgtttcc tccaaagcct aaggatacct tgatgatctc caggactcct gaagtcacat 4620
gtgtagttgt ggatgtgagc catgaagatc ctgaggtgaa gttcaactgg tatgtggatg 4680
gtgtggaagt gcacaatgcc aagacaaagc cgagagagga acagtacaac agcacgtaca 4740
gggttgtctc agttctcact gttctccatc aagattggtt gaatggcaaa gagtacaagt 4800
gcaaggtctc caacaaagcc ctcccagccc ccattgagaa gaccatttcc aaagcgaaag 4860
ggcaaccccg tgaaccacaa gtgtacacac ttcctccatc tcgcgatgaa ctgaccaaga 4920
accaggtcag cttgacttgc ctggtgaaag gcttctatcc ctctgacata gctgtagagt 4980
gggagagcaa tgggcaaccg gagaacaact acaagactac acctcccgtt ctcgattctg 5040
acggctcctt cttcctctac agcaagctca cagtggacaa gagcaggtgg caacaaggga 5100
atgtcttctc atgctccgtg atgcatgagg ctcttcacaa tcactacaca cagaagagtc 5160
tctccttgtc tccgggtaaa ggaggtggcg gatcaggtgg aggcggttca ggcggaggtg 5220
gatccaaggg cgtgtcatac tccttgtgta ccgctgcctt cacattcacc aagatcccgg 5280
ctgaaacact ccacggaacc gttaccgtgg aggtccaata cgccggtaca gatggacctt 5340
gcaaggttcc agctcagatg gcggtggaca tgcaaactct taccccagtt ggaaggttga 5400
ttaccgctaa ccccgttatc actgaaagca ctgagaactc taagatgatg ttggaacttg 5460
atccaccatt cggtgactct tacattgtca ttggtgtggg agagaagaag atcacccacc 5520
actggcacag gagtggtagc acttaagagc tcgaagtgac atcacaaagt tgaaggtaat 5580
aaagccaaat taattaagac attttcataa tgatgtcaag aatgcaaagc aaattgcata 5640
actgccttta tgcaaaacat taatataata taaattataa agaactgcgc tctctgcttc 5700
ttattttctt agcttcattt attagtcact agctgttcag aattttcagt atcttttgat 5760
attactaaga acctaatcac acaatgtata ttcttatgca ggaaaagcag aatgctgagc 5820
taaaagaaag gctttttcca ttttcgagag acaatgagaa aagaagaaga agaagaagaa 5880
gaagaagaag aagaaaagag taaataataa agccccacag gaggcgaagt tcttgtagct 5940
ccatgttatc taagttattg atattgtttg ccctatattt tatttctgtc attgtgtatg 6000
ttttgttcag tttcgatctc cttgcaaaat gcagagatta tgagatgaat aaactaagtt 6060
atattattat acgtgttaat attctcctcc tctctctagc tagccttttg ttttctcttt 6120
ttcttatttg attttcttta aatcaatcca ttttaggaga gggccaggga gtgatccagc 6180
aaaacatgaa gattagaaga aacttccctc ttttttttcc tgaaaacaat ttaacgtcga 6240
gatttatctc tttttgtaat ggaatcattt ctacagttat gacgaattgt acatcaacga 6300
aaaattagtc aaacgactaa aataaataaa tatcatgtgt tattaagaaa attctcctat 6360
aagaatattt taatagatca tatgtttgta aaaaaaatta atttttacta acacatatat 6420
ttacttatca aaaatttgac aaagtaagat taaaataata ttcatctaac aaaaaaaaaa 6480
ccagaaaatg ctgaaaaccc ggcaaaaccg aaccaatcca aaccgatata gttggtttgg 6540
tttgattttg atataaaccg aaccaactcg gtccatttgc acccctaatc ataatagctt 6600
taatatttca agatattatt aagttaacgt tgtcaatatc ctggaaattt tgcaaaatga 6660
atcaagccta tatggctgta atatgaattt aaaagcagct cgatgtggtg gtaatatgta 6720
atttacttga ttctaaaaaa atatcccaag tattaataat ttctgctagg aagaaggtta 6780
gctacgattt acagcaaagc cagaatacaa agaaccataa agtgattgaa gctcgaaata 6840
tacgaaggaa caaatatttt taaaaaaata cgcaatgact tggaacaaaa gaaagtgata 6900
tattttttgt tcttaaacaa gcatcccctc taaagaatgg cagttttcct ttgcatgtaa 6960
ctattatgct cccttcgtta caaaaatttt ggactactat tgggaacttc ttctgaaaat 7020
agtggtaccg agtgtacttc aagtcagttg gaaatcaata aaatgattat tttatgaata 7080
tatttcattg tgcaagtaga tagaaattac atatgttaca taacacacga aataaacaaa 7140
aaaacacaat ccaaaacaaa caccccaaac aaaataacac tatatatatc ctcgtatgag 7200
gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa aagtctcccc gtcacacata 7260
tagtgggtga cgcaattatc ttcaaagtaa tccttctgtt gacttgtcat tgataacatc 7320
cagtcttcgt caggattgca aagaattata gaagggatcc caccttttat tttcttcttt 7380
tttccatatt tagggttgac agtgaaatca gactggcaac ctattaattg cttccacaat 7440
gggacgaact tgaaggggat gtcgtcgatg atattatagg tggcgtgttc atcgtagttg 7500
gtgaagtcga tggtcccgtt ccagtagttg tgtcgcccga gacttctagc ccaggtggtc 7560
tttccggtac gagttggtcc gcagatgtag aggctggggt gtctgacccc agtccttccc 7620
tcatcctggt tagatcggcc atccactcaa ggtcagattg tgcttgatcg taggagacag 7680
gatgtatgaa agtgtaggca tcgatgctta catgatatag gtgcgtctct ctccagttgt 7740
gcagatcttc gtggcagcgg agatctgatt ctgtgaaggg cgacacgtac tgctcaggtt 7800
gtggaggaaa taatttgttg gctgaatatt ccagccattg aagctttgtt gcccattcat 7860
gagggaactc ttctttgatc atgtcaagat actcctcctt agacgttgca gtctggataa 7920
tagttcgcca tcgtgcgtca gatttgcgag gagacacctt atgatctcgg aaatctcctc 7980
tggttttaat atctccgtcc tttgatatgt aatcaaggac ttgtttagag tttctagctg 8040
gctggatatt agggtgattt ccttcaaaat cgaaaaaaga aggatcccta atacaaggtt 8100
ttttatcaag ctggataaga gcatgatagt gggtagtgcc atcttgatga agctcagaag 8160
caacaccaag gaagaaaata agaaaaggtg tgagtttctc ccagagaaac tggaataaat 8220
catctctttg agatgagcac ttggggtagg taaggaaaac atatttagat tggagtctga 8280
agttcttgct agcagaaggc atgttgttgt gactccgagg ggttgcctca aactctatct 8340
tataaccggc gtggaggcat ggaggcaagg gcattttggt aatttaagta gttagtggaa 8400
aatgacgtca tttacttaaa gacgaagtct tgcgacaagg ggggcccacg ccgaatttta 8460
atattaccgg cgtggcccca ccttatcgcg agtgctttag cacgagcggt ccagatttaa 8520
agtagaaaag ttcccgccca ctagggttaa aggtgttcac actataaaag catatacgat 8580
gtgatggtat ttgatggagc gtatattgta tcaggtattt ccgtcggata cgaattattc 8640
gtacggccgg accggtcccc taggccggcc aattcgagat cggccgcggc tgagtggctc 8700
cttcaatcgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc 8760
gggggtcata acgtgactcc cttaattctc cgctcatgat cagattgtcg tttcccgcct 8820
tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag 8880
cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt 8940
tgtatgtgca tgccaaccac agggttcccc agatctggcg ccggccagcg agacgagcaa 9000
gattggccgc cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc aggcaaattg 9060
caccaacgca tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac 9120
cagatcgcgc aggaggcccg gcagcaccgg cataatcagg ccgatgccga cagcgtcgag 9180
cgcgacagtg ctcagaatta cgatcagggg tatgttgggt ttcacgtctg gcctccggag 9240
actgtcatac gcgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 9300
acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 9360
gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 9420
ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 9480
gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 9540
cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 9600
taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 9660
atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 9720
cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 9780
cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 9840
ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 9900
ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 9960
tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 10020
aaacttggtc tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc 10080
ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat 10140
agacacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg 10200
cagcatcacc cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa 10260
ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa 10320
cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag 10380
aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg 10440
atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag 10500
ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc 10560
acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt 10620
ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc 10680
gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag 10740
ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg 10800
aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat 10860
gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat 10920
tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac 10980
agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc 11040
agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg 11100
gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa 11160
ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc 11220
cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga 11280
ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa 11340
taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt 11400
ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc 11460
ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc 11520
atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag 11580
ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca 11640
agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc 11700
atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc 11760
gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga 11820
cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca 11880
gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc 11940
gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg 12000
cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac 12060
cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc 12120
gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct 12180
gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca 12240
ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct 12300
gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga 12360
cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta 12420
ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc 12480
gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa 12540
gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga 12600
acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt 12660
tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc 12720
tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg 12780
ataaacagag gattaaaatt gacaattcaa tggcaaggac tgccagcgct gccatttttg 12840
gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag 12900
cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac 12960
agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag 13020
acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct 13080
gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca 13140
agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc 13200
agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg 13260
ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc 13320
tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat 13380
ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc 13440
gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca 13500
gcggcgaggg caaccagccc ggtgagcgtc gcaaaggcgc tcggtcttgc cttgctcgtc 13560
gagatctggg gtcgatcagc cggggatgca tcaggccgac agtcggaact tcgggtcccc 13620
gacctgtacc attcggtgag caatggatag gggagttgat atcgtcaacg ttcacttcta 13680
aagaaatagc gccactcagc ttcctcagcg gctttatcca gcgatttcct attatgtcgg 13740
catagttctc aagatcgaca gcctgtcacg gttaagcgag aaatgaataa gaaggctgat 13800
aattcggatc tctgcgaggg agatgatatt tgatcacagg cagcaacgct ctgtcatcgt 13860
tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg 13920
ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc 13980
cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct 14040
gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga 14100
cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt ggtttttctt 14160
ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc 14220
agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttccg 14280
aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc 14340
cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 14400
ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt 14460
cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 14520
ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca 14580
ttcaggctgc gcaactgttg ggaaggg 14607
<210> 30
<211> 14622
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd2-ZHVLnt
<400> 30
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttctcga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctactagt ggtggatcag 4200
gaggttctgg tggttctgga ggttcaggat ccgatgtttt gatgactcaa agccctctct 4260
cacttcctgt gactcttgga cagcccgcat ccatatcttg cagatctagt cagagtattg 4320
ttcatagtaa cggcaacacc tacttggaat ggtatctgca gaaaccaggc cagtctccaa 4380
agcttctgat ctacaaggct tccaatcgtt tctctggtgt cccagacagg tttagtggca 4440
gtggatcagg gactgacttc acattgaaga tcagcagagt tgaggctgaa gatgcgggag 4500
tgtactattg tcttcaaggt tcacatgttc cgtcaacgtt tggaggtggg accaaagtgg 4560
agatcaaggc tagcaccaaa ggtccatcgg tctttccact ggcaccttct tccaagagta 4620
cttctggagg cacagctgca ctgggttgtc ttgtcaagga ctactttcca gaacctgtta 4680
cggtttcgtg gaactcaggt gctctgacca gtggagtgca cacctttcca gctgttcttc 4740
agtcctcagg attgtattct cttagcagtg ttgtgactgt tccatcctca agcttgggca 4800
ctcagaccta catctgcaat gtgaatcaca aacccagcaa caccaaggtt gacaagaaag 4860
ttgagcccaa gtcttgtgac aagactcata cgtgtccacc gtgcccagca cctgaacttc 4920
ttggaggacc gtcagtcttc ttgtttcctc caaagcctaa ggataccttg atgatctcca 4980
ggactcctga agtcacatgt gtagttgtgg atgtgagcca tgaagatcct gaggtgaagt 5040
tcaactggta tgtggatggt gtggaagtgc acaatgccaa gacaaagccg agagaggaac 5100
agtacaacag cacgtacagg gttgtctcag ttctcactgt tctccatcaa gattggttga 5160
atggcaaaga gtacaagtgc aaggtctcca acaaagccct cccagccccc attgagaaga 5220
ccatttccaa agcgaaaggg caaccccgtg aaccacaagt gtacacactt cctccatctc 5280
gcgatgaact gaccaagaac caggtcagct tgacttgcct ggtgaaaggc ttctatccct 5340
ctgacatagc tgtagagtgg gagagcaatg ggcaaccgga gaacaactac aagactacac 5400
ctcccgttct cgattctgac ggctccttct tcctctacag caagctcaca gtggacaaga 5460
gcaggtggca acaagggaat gtcttctcat gctccgtgat gcatgaggct cttcacaatc 5520
actacacaca gaagagtctc tccttgtctc cgggtaaata agagctcgaa gtgacatcac 5580
aaagttgaag gtaataaagc caaattaatt aagacatttt cataatgatg tcaagaatgc 5640
aaagcaaatt gcataactgc ctttatgcaa aacattaata taatataaat tataaagaac 5700
tgcgctctct gcttcttatt ttcttagctt catttattag tcactagctg ttcagaattt 5760
tcagtatctt ttgatattac taagaaccta atcacacaat gtatattctt atgcaggaaa 5820
agcagaatgc tgagctaaaa gaaaggcttt ttccattttc gagagacaat gagaaaagaa 5880
gaagaagaag aagaagaaga agaagaagaa aagagtaaat aataaagccc cacaggaggc 5940
gaagttcttg tagctccatg ttatctaagt tattgatatt gtttgcccta tattttattt 6000
ctgtcattgt gtatgttttg ttcagtttcg atctccttgc aaaatgcaga gattatgaga 6060
tgaataaact aagttatatt attatacgtg ttaatattct cctcctctct ctagctagcc 6120
ttttgttttc tctttttctt atttgatttt ctttaaatca atccatttta ggagagggcc 6180
agggagtgat ccagcaaaac atgaagatta gaagaaactt ccctcttttt tttcctgaaa 6240
acaatttaac gtcgagattt atctcttttt gtaatggaat catttctaca gttatgacga 6300
attgtacatc aacgaaaaat tagtcaaacg actaaaataa ataaatatca tgtgttatta 6360
agaaaattct cctataagaa tattttaata gatcatatgt ttgtaaaaaa aattaatttt 6420
tactaacaca tatatttact tatcaaaaat ttgacaaagt aagattaaaa taatattcat 6480
ctaacaaaaa aaaaaccaga aaatgctgaa aacccggcaa aaccgaacca atccaaaccg 6540
atatagttgg tttggtttga ttttgatata aaccgaacca actcggtcca tttgcacccc 6600
taatcataat agctttaata tttcaagata ttattaagtt aacgttgtca atatcctgga 6660
aattttgcaa aatgaatcaa gcctatatgg ctgtaatatg aatttaaaag cagctcgatg 6720
tggtggtaat atgtaattta cttgattcta aaaaaatatc ccaagtatta ataatttctg 6780
ctaggaagaa ggttagctac gatttacagc aaagccagaa tacaaagaac cataaagtga 6840
ttgaagctcg aaatatacga aggaacaaat atttttaaaa aaatacgcaa tgacttggaa 6900
caaaagaaag tgatatattt tttgttctta aacaagcatc ccctctaaag aatggcagtt 6960
ttcctttgca tgtaactatt atgctccctt cgttacaaaa attttggact actattggga 7020
acttcttctg aaaatagtgg taccgagtgt acttcaagtc agttggaaat caataaaatg 7080
attattttat gaatatattt cattgtgcaa gtagatagaa attacatatg ttacataaca 7140
cacgaaataa acaaaaaaac acaatccaaa acaaacaccc caaacaaaat aacactatat 7200
atatcctcgt atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc 7260
tccccgtcac acatatagtg ggtgacgcaa ttatcttcaa agtaatcctt ctgttgactt 7320
gtcattgata acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct 7380
tttattttct tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt 7440
aattgcttcc acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg 7500
tgttcatcgt agttggtgaa gtcgatggtc ccgttccagt agttgtgtcg cccgagactt 7560
ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtctg 7620
accccagtcc ttccctcatc ctggttagat cggccatcca ctcaaggtca gattgtgctt 7680
gatcgtagga gacaggatgt atgaaagtgt aggcatcgat gcttacatga tataggtgcg 7740
tctctctcca gttgtgcaga tcttcgtggc agcggagatc tgattctgtg aagggcgaca 7800
cgtactgctc aggttgtgga ggaaataatt tgttggctga atattccagc cattgaagct 7860
ttgttgccca ttcatgaggg aactcttctt tgatcatgtc aagatactcc tccttagacg 7920
ttgcagtctg gataatagtt cgccatcgtg cgtcagattt gcgaggagac accttatgat 7980
ctcggaaatc tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt 8040
tagagtttct agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat 8100
ccctaataca aggtttttta tcaagctgga taagagcatg atagtgggta gtgccatctt 8160
gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga 8220
gaaactggaa taaatcatct ctttgagatg agcacttggg gtaggtaagg aaaacatatt 8280
tagattggag tctgaagttc ttgctagcag aaggcatgtt gttgtgactc cgaggggttg 8340
cctcaaactc tatcttataa ccggcgtgga ggcatggagg caagggcatt ttggtaattt 8400
aagtagttag tggaaaatga cgtcatttac ttaaagacga agtcttgcga caaggggggc 8460
ccacgccgaa ttttaatatt accggcgtgg ccccacctta tcgcgagtgc tttagcacga 8520
gcggtccaga tttaaagtag aaaagttccc gcccactagg gttaaaggtg ttcacactat 8580
aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtc 8640
ggatacgaat tattcgtacg gccggaccgg tcccctaggc cggccaattc gagatcggcc 8700
gcggctgagt ggctccttca atcgttgcgg ttctgtcagt tccaaacgta aaacggcttg 8760
tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat 8820
tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa 8880
cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt 8940
atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc tggcgccggc 9000
cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg 9060
tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt gggcggtgac 9120
gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat 9180
gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt tgggtttcac 9240
gtctggcctc cggagactgt catacgcgta aaaaggccgc gttgctggcg tttttccata 9300
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 9360
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 9420
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9480
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9540
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9600
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9660
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9720
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9780
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9840
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 9900
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 9960
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 10020
aaagtatata tgagtaaact tggtctgcag ttgccatgtt ttacggcagt gagagcagag 10080
atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca 10140
ggagggacag ctgatagaca cagaagccac tggagcacct caaaaacacc atcatacact 10200
aaatcagtaa gttggcagca tcacccataa ttgtggtttc aaaatcggct ccgtcgatac 10260
tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt 10320
ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat 10380
ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg 10440
gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct 10500
gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc 10560
cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa 10620
ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg 10680
ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct 10740
gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg 10800
gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat 10860
aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg 10920
cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac 10980
ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat 11040
cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc 11100
agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag 11160
cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat 11220
gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca tcaagtgttt 11280
tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg tattcgtgca 11340
gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt 11400
cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata 11460
agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac 11520
gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc 11580
cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg 11640
ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc 11700
tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc 11760
aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa 11820
aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa 11880
acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc 11940
ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa 12000
caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt 12060
gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca 12120
ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga 12180
gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg 12240
cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga 12300
atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg 12360
ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt 12420
catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag 12480
ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga 12540
ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg 12600
cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg 12660
ccttgtgggg tcagttccgg ctgggggttc agcagccagc gctttactgg catttcagga 12720
acaagcgggc actgctcgac gcacttgctt cgctcagtat cgctcgggac gcacggcgcg 12780
ctctacgaac tgccgataaa cagaggatta aaattgacaa ttcaatggca aggactgcca 12840
gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg 12900
ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg 12960
cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta 13020
aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg 13080
ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca 13140
gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc 13200
aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc 13260
gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga 13320
aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca 13380
acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg 13440
cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag 13500
acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcgcaaa ggcgctcggt 13560
cttgccttgc tcgtcgagat ctggggtcga tcagccgggg atgcatcagg ccgacagtcg 13620
gaacttcggg tccccgacct gtaccattcg gtgagcaatg gataggggag ttgatatcgt 13680
caacgttcac ttctaaagaa atagcgccac tcagcttcct cagcggcttt atccagcgat 13740
ttcctattat gtcggcatag ttctcaagat cgacagcctg tcacggttaa gcgagaaatg 13800
aataagaagg ctgataattc ggatctctgc gagggagatg atatttgatc acaggcagca 13860
acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac 13920
ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc 13980
ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg 14040
attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 14100
gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 14160
ggggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 14220
ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 14280
ttgatggtgg ttccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag 14340
ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 14400
tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat 14460
caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 14520
gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 14580
aaggagcggg cgccattcag gctgcgcaac tgttgggaag gg 14622
<210> 31
<211> 14712
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd2-ZHVLe
<400> 31
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttctcga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctactagt ggtggatcag 4200
gaggttctgg tggttctgga ggttcaggat ccgatgtttt gatgactcaa agccctctct 4260
cacttcctgt gactcttgga cagcccgcat ccatatcttg cagatctagt cagagtattg 4320
ttcatagtaa cggcaacacc tacttggaat ggtatctgca gaaaccaggc cagtctccaa 4380
agcttctgat ctacaaggct tccaatcgtt tctctggtgt cccagacagg tttagtggca 4440
gtggatcagg gactgacttc acattgaaga tcagcagagt tgaggctgaa gatgcgggag 4500
tgtactattg tcttcaaggt tcacatgttc cgtcaacgtt tggaggtggg accaaagtgg 4560
agatcaaggc tagcaccaaa ggtccatcgg tctttccact ggcaccttct tccaagagta 4620
cttctggagg cacagctgca ctgggttgtc ttgtcaagga ctactttcca gaacctgtta 4680
cggtttcgtg gaactcaggt gctctgacca gtggagtgca cacctttcca gctgttcttc 4740
agtcctcagg attgtattct cttagcagtg ttgtgactgt tccatcctca agcttgggca 4800
ctcagaccta catctgcaat gtgaatcaca aacccagcaa caccaaggtt gacaagaaag 4860
ttgagcccaa gtcttgtgac aagactcata cgtgtccacc gtgcccagca cctgaacttc 4920
ttggaggacc gtcagtcttc ttgtttcctc caaagcctaa ggataccttg atgatctcca 4980
ggactcctga agtcacatgt gtagttgtgg atgtgagcca tgaagatcct gaggtgaagt 5040
tcaactggta tgtggatggt gtggaagtgc acaatgccaa gacaaagccg agagaggaac 5100
agtacaacag cacgtacagg gttgtctcag ttctcactgt tctccatcaa gattggttga 5160
atggcaaaga gtacaagtgc aaggtctcca acaaagccct cccagccccc attgagaaga 5220
ccatttccaa agcgaaaggg caaccccgtg aaccacaagt gtacacactt cctccatctc 5280
gcgatgaact gaccaagaac caggtcagct tgacttgcct ggtgaaaggc ttctatccct 5340
ctgacatagc tgtagagtgg gagagcaatg ggcaaccgga gaacaactac aagactacac 5400
ctcccgttct cgattctgac ggctccttct tcctctacag caagctcaca gtggacaaga 5460
gcaggtggca acaagggaat gtcttctcat gctccgtgat gcatgaggct cttcacaatc 5520
actacacaca gaagagtctc tccttgtctc cgggtaaagg aggtggcgga tcaggtggag 5580
gcggttcagg cggaggtgga tcccataaca ctcctgttta caagctggac atatctgagg 5640
caactcaata agagctcgaa gtgacatcac aaagttgaag gtaataaagc caaattaatt 5700
aagacatttt cataatgatg tcaagaatgc aaagcaaatt gcataactgc ctttatgcaa 5760
aacattaata taatataaat tataaagaac tgcgctctct gcttcttatt ttcttagctt 5820
catttattag tcactagctg ttcagaattt tcagtatctt ttgatattac taagaaccta 5880
atcacacaat gtatattctt atgcaggaaa agcagaatgc tgagctaaaa gaaaggcttt 5940
ttccattttc gagagacaat gagaaaagaa gaagaagaag aagaagaaga agaagaagaa 6000
aagagtaaat aataaagccc cacaggaggc gaagttcttg tagctccatg ttatctaagt 6060
tattgatatt gtttgcccta tattttattt ctgtcattgt gtatgttttg ttcagtttcg 6120
atctccttgc aaaatgcaga gattatgaga tgaataaact aagttatatt attatacgtg 6180
ttaatattct cctcctctct ctagctagcc ttttgttttc tctttttctt atttgatttt 6240
ctttaaatca atccatttta ggagagggcc agggagtgat ccagcaaaac atgaagatta 6300
gaagaaactt ccctcttttt tttcctgaaa acaatttaac gtcgagattt atctcttttt 6360
gtaatggaat catttctaca gttatgacga attgtacatc aacgaaaaat tagtcaaacg 6420
actaaaataa ataaatatca tgtgttatta agaaaattct cctataagaa tattttaata 6480
gatcatatgt ttgtaaaaaa aattaatttt tactaacaca tatatttact tatcaaaaat 6540
ttgacaaagt aagattaaaa taatattcat ctaacaaaaa aaaaaccaga aaatgctgaa 6600
aacccggcaa aaccgaacca atccaaaccg atatagttgg tttggtttga ttttgatata 6660
aaccgaacca actcggtcca tttgcacccc taatcataat agctttaata tttcaagata 6720
ttattaagtt aacgttgtca atatcctgga aattttgcaa aatgaatcaa gcctatatgg 6780
ctgtaatatg aatttaaaag cagctcgatg tggtggtaat atgtaattta cttgattcta 6840
aaaaaatatc ccaagtatta ataatttctg ctaggaagaa ggttagctac gatttacagc 6900
aaagccagaa tacaaagaac cataaagtga ttgaagctcg aaatatacga aggaacaaat 6960
atttttaaaa aaatacgcaa tgacttggaa caaaagaaag tgatatattt tttgttctta 7020
aacaagcatc ccctctaaag aatggcagtt ttcctttgca tgtaactatt atgctccctt 7080
cgttacaaaa attttggact actattggga acttcttctg aaaatagtgg taccgagtgt 7140
acttcaagtc agttggaaat caataaaatg attattttat gaatatattt cattgtgcaa 7200
gtagatagaa attacatatg ttacataaca cacgaaataa acaaaaaaac acaatccaaa 7260
acaaacaccc caaacaaaat aacactatat atatcctcgt atgaggagag gcacgttcag 7320
tgactcgacg attcccgagc aaaaaaagtc tccccgtcac acatatagtg ggtgacgcaa 7380
ttatcttcaa agtaatcctt ctgttgactt gtcattgata acatccagtc ttcgtcagga 7440
ttgcaaagaa ttatagaagg gatcccacct tttattttct tcttttttcc atatttaggg 7500
ttgacagtga aatcagactg gcaacctatt aattgcttcc acaatgggac gaacttgaag 7560
gggatgtcgt cgatgatatt ataggtggcg tgttcatcgt agttggtgaa gtcgatggtc 7620
ccgttccagt agttgtgtcg cccgagactt ctagcccagg tggtctttcc ggtacgagtt 7680
ggtccgcaga tgtagaggct ggggtgtctg accccagtcc ttccctcatc ctggttagat 7740
cggccatcca ctcaaggtca gattgtgctt gatcgtagga gacaggatgt atgaaagtgt 7800
aggcatcgat gcttacatga tataggtgcg tctctctcca gttgtgcaga tcttcgtggc 7860
agcggagatc tgattctgtg aagggcgaca cgtactgctc aggttgtgga ggaaataatt 7920
tgttggctga atattccagc cattgaagct ttgttgccca ttcatgaggg aactcttctt 7980
tgatcatgtc aagatactcc tccttagacg ttgcagtctg gataatagtt cgccatcgtg 8040
cgtcagattt gcgaggagac accttatgat ctcggaaatc tcctctggtt ttaatatctc 8100
cgtcctttga tatgtaatca aggacttgtt tagagtttct agctggctgg atattagggt 8160
gatttccttc aaaatcgaaa aaagaaggat ccctaataca aggtttttta tcaagctgga 8220
taagagcatg atagtgggta gtgccatctt gatgaagctc agaagcaaca ccaaggaaga 8280
aaataagaaa aggtgtgagt ttctcccaga gaaactggaa taaatcatct ctttgagatg 8340
agcacttggg gtaggtaagg aaaacatatt tagattggag tctgaagttc ttgctagcag 8400
aaggcatgtt gttgtgactc cgaggggttg cctcaaactc tatcttataa ccggcgtgga 8460
ggcatggagg caagggcatt ttggtaattt aagtagttag tggaaaatga cgtcatttac 8520
ttaaagacga agtcttgcga caaggggggc ccacgccgaa ttttaatatt accggcgtgg 8580
ccccacctta tcgcgagtgc tttagcacga gcggtccaga tttaaagtag aaaagttccc 8640
gcccactagg gttaaaggtg ttcacactat aaaagcatat acgatgtgat ggtatttgat 8700
ggagcgtata ttgtatcagg tatttccgtc ggatacgaat tattcgtacg gccggaccgg 8760
tcccctaggc cggccaattc gagatcggcc gcggctgagt ggctccttca atcgttgcgg 8820
ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg 8880
actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc 8940
agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 9000
tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 9060
accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc 9120
gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag 9180
cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag 9240
gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag 9300
aattacgatc aggggtatgt tgggtttcac gtctggcctc cggagactgt catacgcgta 9360
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 9420
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 9480
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 9540
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 9600
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 9660
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 9720
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 9780
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 9840
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9900
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9960
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 10020
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 10080
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgcag 10140
ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcgg tgcttttgcc 10200
gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaca cagaagccac 10260
tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccataa 10320
ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt 10380
tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt 10440
cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa 10500
taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc 10560
tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat 10620
gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa 10680
cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac 10740
tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc 10800
tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc 10860
atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc 10920
cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac 10980
tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa 11040
aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg 11100
aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg 11160
tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc 11220
gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt 11280
ttactggatg aattgtttta gtacctagat gtggcgcaac gatgccggcg acaagcagga 11340
gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta 11400
tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa 11460
ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac 11520
caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc 11580
aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact 11640
gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg 11700
tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat 11760
cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga 11820
gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac 11880
gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt 11940
cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca 12000
gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac 12060
ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa 12120
caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg 12180
ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat 12240
cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa 12300
tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg 12360
cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt 12420
cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt 12480
gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc 12540
gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct 12600
ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca 12660
ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa 12720
tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc 12780
agcagccagc gctttactgg catttcagga acaagcgggc actgctcgac gcacttgctt 12840
cgctcagtat cgctcgggac gcacggcgcg ctctacgaac tgccgataaa cagaggatta 12900
aaattgacaa ttcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg 12960
cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 13020
cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 13080
ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 13140
ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 13200
aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 13260
gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 13320
aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 13380
gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 13440
gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 13500
aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 13560
ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 13620
agcccggtga gcgtcgcaaa ggcgctcggt cttgccttgc tcgtcgagat ctggggtcga 13680
tcagccgggg atgcatcagg ccgacagtcg gaacttcggg tccccgacct gtaccattcg 13740
gtgagcaatg gataggggag ttgatatcgt caacgttcac ttctaaagaa atagcgccac 13800
tcagcttcct cagcggcttt atccagcgat ttcctattat gtcggcatag ttctcaagat 13860
cgacagcctg tcacggttaa gcgagaaatg aataagaagg ctgataattc ggatctctgc 13920
gagggagatg atatttgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct 13980
accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata 14040
gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc 14100
ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct 14160
gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta 14220
ataacacatt gcggacgttt ttaatgtact ggggtggttt ttcttttcac cagtgagacg 14280
ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg 14340
ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttccgaaatc ggcaaaatcc 14400
cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga 14460
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 14520
atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag 14580
cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 14640
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgccattcag gctgcgcaac 14700
tgttgggaag gg 14712
<210> 32
<211> 14622
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd2- ZHVLhx
<400> 32
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt aagggcgtgt 3480
catactcctt gtgtaccgct gccttcacat tcaccaagat cccggctgaa acactccacg 3540
gaaccgttac cgtggaggtc caatacgccg gtacagatgg accttgcaag gttccagctc 3600
agatggcggt ggacatgcaa actcttaccc cagttggaag gttgattacc gctaaccccg 3660
ttatcactga aagcactgag aactctaaga tgatgttgga acttgatcca ccattcggtg 3720
actcttacat tgtcattggt gtgggagaga agaagatcac ccaccactgg cacaggagtg 3780
gtagcactag tggtggatca ggaggttctg gtggttctgg aggttcagga tccgatgttc 3840
agcttctcga gtctggaggt ggtcttgtgc aacctggagg ttccttgaga ctctcctgtg 3900
cagcttcagg gtttgacttc agtaggtact ggatgagttg ggttcgtcaa gctcctggga 3960
aaggactaga atggattgga gagatcaatc cagattcaag taccatcaac tatactccat 4020
ctctgaagga tcgcttcacc atttccagag acaatgccaa gaacacgttg tatcttcaga 4080
tgaacagctt gaggactgaa gacacagcct tgtactactg cacaagacag ggctatggct 4140
acaactactg gggtcaaggc accactgtca cagtgtcttc agctactagt ggtggatcag 4200
gaggttctgg tggttctgga ggttcaggat ccgatgtttt gatgactcaa agccctctct 4260
cacttcctgt gactcttgga cagcccgcat ccatatcttg cagatctagt cagagtattg 4320
ttcatagtaa cggcaacacc tacttggaat ggtatctgca gaaaccaggc cagtctccaa 4380
agcttctgat ctacaaggct tccaatcgtt tctctggtgt cccagacagg tttagtggca 4440
gtggatcagg gactgacttc acattgaaga tcagcagagt tgaggctgaa gatgcgggag 4500
tgtactattg tcttcaaggt tcacatgttc cgtcaacgtt tggaggtggg accaaagtgg 4560
agatcaaggc tagcaccaaa ggtccatcgg tctttccact ggcaccttct tccaagagta 4620
cttctggagg cacagctgca ctgggttgtc ttgtcaagga ctactttcca gaacctgtta 4680
cggtttcgtg gaactcaggt gctctgacca gtggagtgca cacctttcca gctgttcttc 4740
agtcctcagg attgtattct cttagcagtg ttgtgactgt tccatcctca agcttgggca 4800
ctcagaccta catctgcaat gtgaatcaca aacccagcaa caccaaggtt gacaagaaag 4860
ttgagcccaa gtcttgtgac aagactcata cgtgtccacc gtgcccagca cctgaacttc 4920
ttggaggacc gtcagtcttc ttgtttcctc caaagcctaa ggataccttg atgatctcca 4980
ggactcctga agtcacatgt gtagttgtgg atgtgagcca tgaagatcct gaggtgaagt 5040
tcaactggta tgtggatggt gtggaagtgc acaatgccaa gacaaagccg agagaggaac 5100
agtacaacag cacgtacagg gttgtctcag ttctcactgt tctccatcaa gattggttga 5160
atggcaaaga gtacaagtgc aaggtctcca acaaagccct cccagctcct attgagaaga 5220
ccatctctaa agccaagggt caaccgcgtc gtccacaagt gtacacactt cctccatctc 5280
gcgacgaact caccaagaat caagtcagcc ttacttgtct cgttaagggc ttctatccct 5340
ccgacatcgc tgttgagtgg gagtcaaacg gacaaccgga gaacaattac aagactacac 5400
ctccagtttt ggattccgat ggctccttct tcctctacag caagctcaca gtggacaaat 5460
ctaggtggca acaaggcaac gtcttctcat gctccgtgat gcatggagcc cttcacaatc 5520
actacacaca gaagtacctc tccttgtctc cgggaaagtg agagctcgaa gtgacatcac 5580
aaagttgaag gtaataaagc caaattaatt aagacatttt cataatgatg tcaagaatgc 5640
aaagcaaatt gcataactgc ctttatgcaa aacattaata taatataaat tataaagaac 5700
tgcgctctct gcttcttatt ttcttagctt catttattag tcactagctg ttcagaattt 5760
tcagtatctt ttgatattac taagaaccta atcacacaat gtatattctt atgcaggaaa 5820
agcagaatgc tgagctaaaa gaaaggcttt ttccattttc gagagacaat gagaaaagaa 5880
gaagaagaag aagaagaaga agaagaagaa aagagtaaat aataaagccc cacaggaggc 5940
gaagttcttg tagctccatg ttatctaagt tattgatatt gtttgcccta tattttattt 6000
ctgtcattgt gtatgttttg ttcagtttcg atctccttgc aaaatgcaga gattatgaga 6060
tgaataaact aagttatatt attatacgtg ttaatattct cctcctctct ctagctagcc 6120
ttttgttttc tctttttctt atttgatttt ctttaaatca atccatttta ggagagggcc 6180
agggagtgat ccagcaaaac atgaagatta gaagaaactt ccctcttttt tttcctgaaa 6240
acaatttaac gtcgagattt atctcttttt gtaatggaat catttctaca gttatgacga 6300
attgtacatc aacgaaaaat tagtcaaacg actaaaataa ataaatatca tgtgttatta 6360
agaaaattct cctataagaa tattttaata gatcatatgt ttgtaaaaaa aattaatttt 6420
tactaacaca tatatttact tatcaaaaat ttgacaaagt aagattaaaa taatattcat 6480
ctaacaaaaa aaaaaccaga aaatgctgaa aacccggcaa aaccgaacca atccaaaccg 6540
atatagttgg tttggtttga ttttgatata aaccgaacca actcggtcca tttgcacccc 6600
taatcataat agctttaata tttcaagata ttattaagtt aacgttgtca atatcctgga 6660
aattttgcaa aatgaatcaa gcctatatgg ctgtaatatg aatttaaaag cagctcgatg 6720
tggtggtaat atgtaattta cttgattcta aaaaaatatc ccaagtatta ataatttctg 6780
ctaggaagaa ggttagctac gatttacagc aaagccagaa tacaaagaac cataaagtga 6840
ttgaagctcg aaatatacga aggaacaaat atttttaaaa aaatacgcaa tgacttggaa 6900
caaaagaaag tgatatattt tttgttctta aacaagcatc ccctctaaag aatggcagtt 6960
ttcctttgca tgtaactatt atgctccctt cgttacaaaa attttggact actattggga 7020
acttcttctg aaaatagtgg taccgagtgt acttcaagtc agttggaaat caataaaatg 7080
attattttat gaatatattt cattgtgcaa gtagatagaa attacatatg ttacataaca 7140
cacgaaataa acaaaaaaac acaatccaaa acaaacaccc caaacaaaat aacactatat 7200
atatcctcgt atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc 7260
tccccgtcac acatatagtg ggtgacgcaa ttatcttcaa agtaatcctt ctgttgactt 7320
gtcattgata acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct 7380
tttattttct tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt 7440
aattgcttcc acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg 7500
tgttcatcgt agttggtgaa gtcgatggtc ccgttccagt agttgtgtcg cccgagactt 7560
ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtctg 7620
accccagtcc ttccctcatc ctggttagat cggccatcca ctcaaggtca gattgtgctt 7680
gatcgtagga gacaggatgt atgaaagtgt aggcatcgat gcttacatga tataggtgcg 7740
tctctctcca gttgtgcaga tcttcgtggc agcggagatc tgattctgtg aagggcgaca 7800
cgtactgctc aggttgtgga ggaaataatt tgttggctga atattccagc cattgaagct 7860
ttgttgccca ttcatgaggg aactcttctt tgatcatgtc aagatactcc tccttagacg 7920
ttgcagtctg gataatagtt cgccatcgtg cgtcagattt gcgaggagac accttatgat 7980
ctcggaaatc tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt 8040
tagagtttct agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat 8100
ccctaataca aggtttttta tcaagctgga taagagcatg atagtgggta gtgccatctt 8160
gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga 8220
gaaactggaa taaatcatct ctttgagatg agcacttggg gtaggtaagg aaaacatatt 8280
tagattggag tctgaagttc ttgctagcag aaggcatgtt gttgtgactc cgaggggttg 8340
cctcaaactc tatcttataa ccggcgtgga ggcatggagg caagggcatt ttggtaattt 8400
aagtagttag tggaaaatga cgtcatttac ttaaagacga agtcttgcga caaggggggc 8460
ccacgccgaa ttttaatatt accggcgtgg ccccacctta tcgcgagtgc tttagcacga 8520
gcggtccaga tttaaagtag aaaagttccc gcccactagg gttaaaggtg ttcacactat 8580
aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtc 8640
ggatacgaat tattcgtacg gccggaccgg tcccctaggc cggccaattc gagatcggcc 8700
gcggctgagt ggctccttca atcgttgcgg ttctgtcagt tccaaacgta aaacggcttg 8760
tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat 8820
tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa 8880
cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt 8940
atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc tggcgccggc 9000
cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg 9060
tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt gggcggtgac 9120
gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat 9180
gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt tgggtttcac 9240
gtctggcctc cggagactgt catacgcgta aaaaggccgc gttgctggcg tttttccata 9300
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 9360
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 9420
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9480
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9540
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9600
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9660
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9720
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9780
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9840
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 9900
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 9960
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 10020
aaagtatata tgagtaaact tggtctgcag ttgccatgtt ttacggcagt gagagcagag 10080
atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca 10140
ggagggacag ctgatagaca cagaagccac tggagcacct caaaaacacc atcatacact 10200
aaatcagtaa gttggcagca tcacccataa ttgtggtttc aaaatcggct ccgtcgatac 10260
tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt 10320
ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat 10380
ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg 10440
gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct 10500
gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc 10560
cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa 10620
ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg 10680
ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct 10740
gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg 10800
gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat 10860
aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg 10920
cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac 10980
ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat 11040
cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc 11100
agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag 11160
cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat 11220
gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca tcaagtgttt 11280
tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg tattcgtgca 11340
gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt 11400
cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata 11460
agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac 11520
gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc 11580
cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg 11640
ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc 11700
tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc 11760
aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa 11820
aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa 11880
acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc 11940
ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa 12000
caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt 12060
gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca 12120
ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga 12180
gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg 12240
cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga 12300
atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg 12360
ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt 12420
catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag 12480
ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga 12540
ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg 12600
cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg 12660
ccttgtgggg tcagttccgg ctgggggttc agcagccagc gctttactgg catttcagga 12720
acaagcgggc actgctcgac gcacttgctt cgctcagtat cgctcgggac gcacggcgcg 12780
ctctacgaac tgccgataaa cagaggatta aaattgacaa ttcaatggca aggactgcca 12840
gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg 12900
ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg 12960
cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta 13020
aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg 13080
ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca 13140
gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc 13200
aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc 13260
gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga 13320
aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca 13380
acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg 13440
cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag 13500
acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcgcaaa ggcgctcggt 13560
cttgccttgc tcgtcgagat ctggggtcga tcagccgggg atgcatcagg ccgacagtcg 13620
gaacttcggg tccccgacct gtaccattcg gtgagcaatg gataggggag ttgatatcgt 13680
caacgttcac ttctaaagaa atagcgccac tcagcttcct cagcggcttt atccagcgat 13740
ttcctattat gtcggcatag ttctcaagat cgacagcctg tcacggttaa gcgagaaatg 13800
aataagaagg ctgataattc ggatctctgc gagggagatg atatttgatc acaggcagca 13860
acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac 13920
ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc 13980
ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg 14040
attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg 14100
gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact 14160
ggggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 14220
ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 14280
ttgatggtgg ttccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag 14340
ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 14400
tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat 14460
caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 14520
gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 14580
aaggagcggg cgccattcag gctgcgcaac tgttgggaag gg 14622
<210> 33
<211> 13178
<212> DNA
<213> 人工序列
<220>
<223> pBYKEMd-6D8K
<400> 33
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgataa agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcattc 3420
tcttcttggt agccacagct acaggtgtcc actccgatgt tttgatgact caaagccctc 3480
tctcacttcc tgtgactctt ggacagcccg catccatatc ttgcagatct agtcagagta 3540
ttgttcatag taacggcaac acctacttgg aatggtatct gcagaaacca ggccagtctc 3600
caaagcttct gatctacaag gcttccaatc gtttctctgg tgtcccagac aggtttagtg 3660
gcagtggatc agggactgac ttcacattga agatcagcag agttgaggct gaagatgcgg 3720
gagtgtacta ttgtcttcaa ggttcacatg ttccgtcaac gtttggaggt gggaccaaag 3780
tggagatcaa gactgttgcg gcgccatctg tcttcatctt tcctccatct gatgaacaac 3840
tcaagtctgg aactgcttct gttgtgtgcc ttctgaacaa cttctatcct agagaagcca 3900
aagtacagtg gaaggttgac aatgctcttc aatcaggtaa ctcccaggag agtgtcacag 3960
agcaagattc caaggattcc acctacagcc tctcaagtac cttgacgttg agcaaggcag 4020
actatgagaa acacaaagtg tacgcatgcg aagtcactca tcagggcctg tcatcacccg 4080
tgacaaagag cttcaacagg ggagagtgtt aggtaccgag ctcgaagtga catcacaaag 4140
ttgaaggtaa taaagccaaa ttaattaaga cattttcata atgatgtcaa gaatgcaaag 4200
caaattgcat aactgccttt atgcaaaaca ttaatataat ataaattata aagaactgcg 4260
ctctctgctt cttattttct tagcttcatt tattagtcac tagctgttca gaattttcag 4320
tatcttttga tattactaag aacctaatca cacaatgtat attcttatgc aggaaaagca 4380
gaatgctgag ctaaaagaaa ggctttttcc attttcgaga gacaatgaga aaagaagaag 4440
aagaagaaga agaagaagaa gaagaaaaga gtaaataata aagccccaca ggaggcgaag 4500
ttcttgtagc tccatgttat ctaagttatt gatattgttt gccctatatt ttatttctgt 4560
cattgtgtat gttttgttca gtttcgatct ccttgcaaaa tgcagagatt atgagatgaa 4620
taaactaagt tatattatta tacgtgttaa tattctcctc ctctctctag ctagcctttt 4680
gttttctctt tttcttattt gattttcttt aaatcaatcc attttaggag agggccaggg 4740
agtgatccag caaaacatga agattagaag aaacttccct cttttttttc ctgaaaacaa 4800
tttaacgtcg agatttatct ctttttgtaa tggaatcatt tctacagtta tgacgaattg 4860
tacatcaacg aaaaattagt caaacgacta aaataaataa atatcatgtg ttattaagaa 4920
aattctccta taagaatatt ttaatagatc atatgtttgt aaaaaaaatt aatttttact 4980
aacacatata tttacttatc aaaaatttga caaagtaaga ttaaaataat attcatctaa 5040
caaaaaaaaa accagaaaat gctgaaaacc cggcaaaacc gaaccaatcc aaaccgatat 5100
agttggtttg gtttgatttt gatataaacc gaaccaactc ggtccatttg cacccctaat 5160
cataatagct ttaatatttc aagatattat taagttaacg ttgtcaatat cctggaaatt 5220
ttgcaaaatg aatcaagcct atatggctgt aatatgaatt taaaagcagc tcgatgtggt 5280
ggtaatatgt aatttacttg attctaaaaa aatatcccaa gtattaataa tttctgctag 5340
gaagaaggtt agctacgatt tacagcaaag ccagaataca aagaaccata aagtgattga 5400
agctcgaaat atacgaagga acaaatattt ttaaaaaaat acgcaatgac ttggaacaaa 5460
agaaagtgat atattttttg ttcttaaaca agcatcccct ctaaagaatg gcagttttcc 5520
tttgcatgta actattatgc tcccttcgtt acaaaaattt tggactacta ttgggaactt 5580
cttctgaaaa tagtggtacc gagtgtactt caagtcagtt ggaaatcaat aaaatgatta 5640
ttttatgaat atatttcatt gtgcaagtag atagaaatta catatgttac ataacacacg 5700
aaataaacaa aaaaacacaa tccaaaacaa acaccccaaa caaaataaca ctatatatat 5760
cctcgtatga ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc 5820
cgtcacacat atagtgggtg acgcaattat cttcaaagta atccttctgt tgacttgtca 5880
ttgataacat ccagtcttcg tcaggattgc aaagaattat agaagggatc ccacctttta 5940
ttttcttctt ttttccatat ttagggttga cagtgaaatc agactggcaa cctattaatt 6000
gcttccacaa tgggacgaac ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt 6060
catcgtagtt ggtgaagtcg atggtcccgt tccagtagtt gtgtcgcccg agacttctag 6120
cccaggtggt ctttccggta cgagttggtc cgcagatgta gaggctgggg tgtctgaccc 6180
cagtccttcc ctcatcctgg ttagatcggc catccactca aggtcagatt gtgcttgatc 6240
gtaggagaca ggatgtatga aagtgtaggc atcgatgctt acatgatata ggtgcgtctc 6300
tctccagttg tgcagatctt cgtggcagcg gagatctgat tctgtgaagg gcgacacgta 6360
ctgctcaggt tgtggaggaa ataatttgtt ggctgaatat tccagccatt gaagctttgt 6420
tgcccattca tgagggaact cttctttgat catgtcaaga tactcctcct tagacgttgc 6480
agtctggata atagttcgcc atcgtgcgtc agatttgcga ggagacacct tatgatctcg 6540
gaaatctcct ctggttttaa tatctccgtc ctttgatatg taatcaagga cttgtttaga 6600
gtttctagct ggctggatat tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct 6660
aatacaaggt tttttatcaa gctggataag agcatgatag tgggtagtgc catcttgatg 6720
aagctcagaa gcaacaccaa ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa 6780
ctggaataaa tcatctcttt gagatgagca cttggggtag gtaaggaaaa catatttaga 6840
ttggagtctg aagttcttgc tagcagaagg catgttgttg tgactccgag gggttgcctc 6900
aaactctatc ttataaccgg cgtggaggca tggaggcaag ggcattttgg taatttaagt 6960
agttagtgga aaatgacgtc atttacttaa agacgaagtc ttgcgacaag gggggcccac 7020
gccgaatttt aatattaccg gcgtggcccc accttatcgc gagtgcttta gcacgagcgg 7080
tccagattta aagtagaaaa gttcccgccc actagggtta aaggtgttca cactataaaa 7140
gcatatacga tgtgatggta tttgatggag cgtatattgt atcaggtatt tccgtcggat 7200
acgaattatt cgtacggccg gaccggtccc ctaggccggc caattcgaga tcggccgcgg 7260
ctgagtggct ccttcaatcg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc 7320
gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc 7380
gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 7440
agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc 7500
gttcgtccat ttgtatgtgc atgccaacca cagggttccc cagatctggc gccggccagc 7560
gagacgagca agattggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg 7620
caggcaaatt gcaccaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg 7680
ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg 7740
acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtatgttggg tttcacgtct 7800
ggcctccgga gactgtcata cgcgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7860
ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7920
aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7980
gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8040
tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8100
tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8160
gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8220
cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8280
cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8340
agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8400
caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8460
ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8520
aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8580
tatatatgag taaacttggt ctgcagttgc catgttttac ggcagtgaga gcagagatag 8640
cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag 8700
ggacagctga tagacacaga agccactgga gcacctcaaa aacaccatca tacactaaat 8760
cagtaagttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt cgatactatg 8820
ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag 8880
aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt 8940
aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat 9000
tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta 9060
aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt 9120
ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa 9180
agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca 9240
tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa 9300
agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt 9360
gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg 9420
atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg 9480
agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg 9540
acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg 9600
ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg 9660
aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg 9720
attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatgtgg 9780
cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc 9840
tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc 9900
aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt 9960
gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg 10020
cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt 10080
gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa 10140
accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg 10200
atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc 10260
ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt 10320
ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc 10380
gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac 10440
acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac 10500
acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag 10560
aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag 10620
atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg 10680
ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt 10740
tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg 10800
tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg 10860
gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag 10920
gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata 10980
tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg 11040
caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc 11100
acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc 11160
ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt 11220
gtggggtcag ttccggctgg gggttcagca gccagcgctt tactggcatt tcaggaacaa 11280
gcgggcactg ctcgacgcac ttgcttcgct cagtatcgct cgggacgcac ggcgcgctct 11340
acgaactgcc gataaacaga ggattaaaat tgacaattca atggcaagga ctgccagcgc 11400
tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag 11460
gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg 11520
gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag 11580
caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg 11640
attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac 11700
tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt 11760
gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt 11820
aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc 11880
gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt 11940
ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc 12000
cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg 12060
ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cgcaaaggcg ctcggtcttg 12120
ccttgctcgt cgagatctgg ggtcgatcag ccggggatgc atcaggccga cagtcggaac 12180
ttcgggtccc cgacctgtac cattcggtga gcaatggata ggggagttga tatcgtcaac 12240
gttcacttct aaagaaatag cgccactcag cttcctcagc ggctttatcc agcgatttcc 12300
tattatgtcg gcatagttct caagatcgac agcctgtcac ggttaagcga gaaatgaata 12360
agaaggctga taattcggat ctctgcgagg gagatgatat ttgatcacag gcagcaacgc 12420
tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg 12480
cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac 12540
aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt 12600
tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt 12660
aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg 12720
tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct 12780
gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga 12840
tggtggttcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt 12900
gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 12960
agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag 13020
ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 13080
tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 13140
agcgggcgcc attcaggctg cgcaactgtt gggaaggg 13178
<210> 34
<211> 16
<212> PRT
<213> 诺如病毒
<400> 34
Leu Pro Gln Glu Trp Val Gln Tyr Phe Tyr Gln Glu Ala Ala Pro Ala
1 5 10 15
<210> 35
<211> 20
<212> PRT
<213> 诺如病毒
<400> 35
Leu Pro Gln Glu Trp Val Gln Tyr Phe Tyr Gln Glu Ala Ala Pro Ala
1 5 10 15
Gln Ser Val Ala
20
<210> 36
<211> 20
<212> PRT
<213> 诺如病毒
<400> 36
Leu Pro Gln Glu Phe Val Ser Trp Phe Ala Ser Asn Ala Phe Thr Val
1 5 10 15
Gln Ser Glu Ala
20
<210> 37
<211> 16
<212> PRT
<213> 诺如病毒
<400> 37
Ala Leu Leu Arg Phe Val Asn Pro Asp Thr Gly Arg Val Leu Phe Glu
1 5 10 15
<210> 38
<211> 16
<212> PRT
<213> 诺如病毒
<400> 38
Ala Leu Leu His Tyr Val Asp Pro Asp Thr Asn Arg Asn Leu Gly Glu
1 5 10 15
<210> 39
<211> 16
<212> PRT
<213> 诺如病毒
<400> 39
Leu Leu Leu Arg Tyr Arg Asn Thr Leu Thr Gly Gln Leu Leu Phe Glu
1 5 10 15
<210> 40
<211> 12
<212> PRT
<213> 诺如病毒
<400> 40
Val Ser Trp Val Ser Arg Phe Tyr Gln Leu Lys Pro
1 5 10
<210> 41
<211> 12
<212> PRT
<213> 诺如病毒
<400> 41
Asp Ser Trp Val Asn Gln Phe Tyr Thr Leu Ala Pro
1 5 10
<210> 42
<211> 12
<212> PRT
<213> 诺如病毒
<400> 42
Val Ser Trp Val Pro Arg Leu Tyr Gln Leu Ala Ser
1 5 10
<210> 43
<211> 20
<212> PRT
<213> 诺如病毒
<400> 43
Leu Pro Gln Glu Tyr Ile Thr His Phe Val Ser Glu Gln Ala Pro Thr
1 5 10 15
Val Gly Glu Ala
20
<210> 44
<211> 9
<212> PRT
<213> 诺如病毒
<400> 44
Ser Trp Val Ser Arg Phe Tyr Gln Leu
1 5
<210> 45
<211> 12
<212> PRT
<213> 诺如病毒
<400> 45
Gln Asn Val Ile Asp Pro Trp Ile Arg Asn Asn Phe
1 5 10
<210> 46
<211> 17
<212> PRT
<213> 诺如病毒
<400> 46
Gln Ala Pro Gly Gly Glu Phe Thr Val Ser Pro Arg Asn Ala Pro Gly
1 5 10 15
Glu
<210> 47
<211> 9
<212> PRT
<213> 诺如病毒
<400> 47
Lys Val Ile Phe Ala Ala Val Pro Pro
1 5
<210> 48
<211> 9
<212> PRT
<213> 诺如病毒
<400> 48
Lys Val Val Val Ala Leu Val Pro Pro
1 5
<210> 49
<211> 9
<212> PRT
<213> 诺如病毒
<400> 49
Lys Ile Ile Val Cys Cys Val Pro Pro
1 5
<210> 50
<211> 12
<212> PRT
<213> 诺如病毒
<400> 50
Val Asn Met Ile Asp Pro Trp Ile Val Asn Asn Phe
1 5 10
<210> 51
<211> 12
<212> PRT
<213> 诺如病毒
<400> 51
Ile Asn Gln Ile Asp Pro Trp Ile Phe Gln Asn Phe
1 5 10
<210> 52
<211> 17
<212> PRT
<213> 诺如病毒
<400> 52
Gln Ser Pro Gln Gly Glu Phe Thr Ile Ser Pro Asn Asn Thr Pro Gly
1 5 10 15
Asp
<210> 53
<211> 17
<212> PRT
<213> 诺如病毒
<400> 53
Gln Cys Pro Leu Gly Glu Phe Ser Ile Ser Pro Arg Asn Thr Pro Gly
1 5 10 15
Glu
<210> 54
<211> 10
<212> PRT
<213> 诺如病毒
<400> 54
Thr Met Phe Pro His Ile Ile Val Asp Val
1 5 10
<210> 55
<211> 131
<212> PRT
<213> 诺如病毒
<400> 55
Met Arg Met Ala Thr Pro Ser Ser Ala Pro Ser Val Arg Asn Thr Glu
1 5 10 15
Lys Arg Lys Asn Lys Lys Ala Ser Ser Lys Ala Ser Val Ser Phe Gly
20 25 30
Ala Pro Ser Pro Leu Ser Ser Glu Ser Glu Asp Glu Ile Asn Tyr Met
35 40 45
Thr Pro Pro Glu Gln Glu Ala Gln Pro Gly Ala Leu Ala Ala Leu His
50 55 60
Ala Glu Gly Pro Leu Ala Gly Leu Pro Val Thr Arg Ser Asp Ala Arg
65 70 75 80
Val Leu Ile Phe Asn Glu Trp Glu Glu Arg Lys Lys Ser Asp Pro Trp
85 90 95
Leu Arg Leu Asp Met Ser Asp Lys Ala Ile Phe Arg Arg Tyr Pro His
100 105 110
Leu Arg Pro Lys Glu Asp Arg Pro Asp Ala Pro Ser His Ala Glu Asp
115 120 125
Ala Met Asp
130
<210> 56
<211> 134
<212> PRT
<213> 诺如病毒
<400> 56
Met Lys Met Ala Ser Asn Asp Ala Ser Ala Ala Ala Val Ala Asn Ser
1 5 10 15
Asn Asn Asp Ile Ala Lys Ser Ser Ser Asp Gly Val Leu Ser Asn Met
20 25 30
Ala Val Thr Phe Lys Arg Ala Leu Gly Ala Arg Pro Lys Gln Pro Pro
35 40 45
Pro Lys Glu Ile Pro Pro Arg Pro Pro Arg Pro Pro Thr Pro Glu Leu
50 55 60
Val Lys Lys Ile Pro Pro Pro Pro Pro Asn Gly Glu Asp Glu Leu Val
65 70 75 80
Val Ser Tyr Ser Ala Lys Asp Gly Val Ser Gly Leu Pro Glu Leu Thr
85 90 95
Thr Val Arg Gln Pro Glu Glu Thr Asn Thr Ala Phe Ser Val Pro Pro
100 105 110
Leu Asn Gln Arg Glu Ser Arg Asp Ala Lys Glu Pro Leu Thr Gly Thr
115 120 125
Ile Ile Glu Met Trp Asp
130
<210> 57
<211> 169
<212> PRT
<213> 诺如病毒
<400> 57
Met Met Met Ala Ser Asn Asp Ala Ile Ala Val Ala Asp Val Ser Asn
1 5 10 15
Asn Asn Ala Asn Thr Asn Asn Asp Asn Ile Gly Ser Arg Leu Met Ala
20 25 30
Arg Ile Arg Gly Arg Ile Gly Pro Gln Arg Gly Glu Thr Thr Thr Lys
35 40 45
Ile Lys Asp Ala Asn Met Ala Leu Asp Leu Leu Arg Arg Ser Gln Thr
50 55 60
Pro Ser Pro Ser Arg Gln Glu Ser Pro Pro Lys Ser Gln Arg Asp Arg
65 70 75 80
Pro Pro Arg Thr Ala Ser Glu Val Lys Lys Val Met Gly Trp Asp Ala
85 90 95
Glu Pro Glu His Gln Glu Ser Thr Ala Lys Ala Trp Ser Asp Leu Ala
100 105 110
Gln Glu Glu Lys Glu Glu Ile Met Arg Asn Asn Glu Lys Leu Phe Asp
115 120 125
Ala Gly Gly Ile Thr Pro Ser Thr Leu Pro Ser Thr Phe Glu Arg Ala
130 135 140
Asp Pro Ile Asp Ser Pro Ser Glu Gln Gln Pro Val Ile Trp Ser Ala
145 150 155 160
Ser Gly Gly Val Asp Ile Gly Val Asn
165
<210> 58
<211> 10
<212> PRT
<213> 诺如病毒
<400> 58
Thr Leu Phe Pro His Val Ile Ala Asp Val
1 5 10
<210> 59
<211> 10
<212> PRT
<213> 诺如病毒
<400> 59
Thr Met Phe Pro His Ile Ile Val Asp Val
1 5 10
<210> 60
<211> 10
<212> PRT
<213> 诺如病毒
<400> 60
Thr Cys Phe Pro His Val Met Cys Asp Val
1 5 10
<210> 61
<211> 12
<212> PRT
<213> 诺如病毒
<400> 61
Leu Gln Leu Gly Pro His Leu Asn Pro Phe Leu Ser
1 5 10
<210> 62
<211> 12
<212> PRT
<213> 诺如病毒
<400> 62
Ala Pro Leu Gly Pro Asp Leu Asn Pro Tyr Leu Ser
1 5 10
<210> 63
<211> 12
<212> PRT
<213> 诺如病毒
<400> 63
Leu Ala Leu Gly Pro Gly Leu Asn Pro Tyr Leu Ala
1 5 10
<210> 64
<211> 9
<212> PRT
<213> 诺如病毒
<400> 64
Ser Trp Val Ser Arg Phe Tyr Gln Leu
1 5
<210> 65
<211> 9
<212> PRT
<213> 诺如病毒
<400> 65
Ser Trp Val Asn Gln Phe Tyr Thr Leu
1 5
<210> 66
<211> 9
<212> PRT
<213> 诺如病毒
<400> 66
Ser Trp Val Pro Arg Leu Tyr Gln Leu
1 5
<210> 67
<211> 14
<212> PRT
<213> 诺如病毒
<400> 67
Phe Val Ser Glu Gln Ala Pro Thr Met Gly Asp Ala Ala Leu
1 5 10
<210> 68
<211> 14
<212> PRT
<213> 诺如病毒
<400> 68
Phe Tyr Gln Glu Ala Ala Pro Ala Gln Ser Asp Val Ala Leu
1 5 10
<210> 69
<211> 14
<212> PRT
<213> 诺如病毒
<400> 69
Phe Ala Ser Asn Ala Phe Thr Val Gln Ser Glu Ala Leu Leu
1 5 10
<210> 70
<211> 10
<212> PRT
<213> 诺如病毒
<400> 70
Ser Pro Ala Pro Val Gly Phe Pro Asp Phe
1 5 10
<210> 71
<211> 10
<212> PRT
<213> 诺如病毒
<400> 71
Ile Pro Ala Pro Leu Gly Thr Pro Asp Phe
1 5 10
<210> 72
<211> 10
<212> PRT
<213> 诺如病毒
<400> 72
Arg Ala Ala Pro Leu Gly Tyr Pro Asp Phe
1 5 10
<210> 73
<211> 16323
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 73
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt atgaggatgg 3480
ctacaccttc ttccgctccc tcagttagaa acactgagaa gaggaagaac aagaaagcgt 3540
catccaaggc ttctgtttct ttcggggcac cttccccact ttcttctgag tctgaggacg 3600
agatcaacta catgactcct ccagaacaag aggcgcagcc tggtgccttg gctgctttgc 3660
acgccgaggg accattggcc ggacttccag ttactaggtc agatgccaga gtacttatct 3720
tcaacgagtg ggaagaaaga aagaagtccg atccctggct cagattagac atgtccgaca 3780
aagccatctt cagacgttat cctcaccttc gtcctaagga ggacagacca gacgcaccat 3840
cacacgccga ggacgccatg gacggtggat ctggagggtc tggaggagga ggatctggag 3900
gctctggagg atctatgaag atggcctcca atgatgcttc tgctgctgct gtcgcgaatt 3960
ccaacaatga catcgcaaaa tcttcctccg acggcgtttt gtccaacatg gctgttactt 4020
tcaagcgtgc tttgggagct agaccaaaac agccaccacc taaggagatc ccaccaagac 4080
ctcctcgtcc gcccactcca gagttggtta agaaaatccc acctccacca ccgaacggag 4140
aggatgagct tgtcgtttct tactctgcta aggacggagt ttctggactt ccagagctta 4200
ctacagttcg tcagccagaa gagaccaaca ctgccttctc tgttccacca ttgaaccaga 4260
gggagtccag agacgccaag gagcctctta ctggaactat catcgagatg tgggacggag 4320
gctctggtgg atctggcgga ggcggctctg gaggatctgg tggatctatg atgatggctt 4380
ccaacgacgc gatcgcagta gcggacgttt ccaacaataa cgcaaacacc aacaatgata 4440
acatcggttc tcgtctcatg gctagaatca gagggagaat cggaccacag agaggagaga 4500
ctaccactaa gatcaaggac gcaaacatgg ccttggatct cttgcgtaga agccaaacac 4560
catccccatc ccgtcaagag tccccaccca agtctcaaag agacagacct cccagaactg 4620
cctctgaagt taagaaggtt atgggttggg acgctgagcc ggaacatcaa gaatctactg 4680
ccaaggcttg gtccgatctt gcacaagaag agaaggagga gatcatgaga aacaacgaga 4740
aacttttcga cgccggagga attactccat ctactcttcc atctactttt gaaagagccg 4800
atcctatcga ctcaccatct gagcaacagc ctgttatttg gtctgcctcc ggaggtgtgg 4860
acatcggagt caacactagt ggtggatcag gaggttctgg tggttctgga ggttcaggat 4920
ccgatgttca gcttcttgag tctggaggtg gtcttgtgca acctggaggt tccttgagac 4980
tctcctgtgc agcttcaggg tttgacttca gtaggtactg gatgagttgg gttcgtcaag 5040
ctcctgggaa aggactagaa tggattggag agatcaatcc agattcaagt accatcaact 5100
atactccatc tctgaaggat cgcttcacca tttccagaga caatgccaag aacacgttgt 5160
atcttcagat gaacagcttg aggactgaag acacagcctt gtactactgc acaagacagg 5220
gctatggcta caactactgg ggtcaaggca ccactgtcac agtgtcttca gctagcacca 5280
aaggtccatc ggtctttcca ctggcacctt cttccaagag tacttctgga ggcacagctg 5340
cactgggttg tcttgtcaag gactactttc cagaacctgt tacggtttcg tggaactcag 5400
gtgctctgac cagtggagtg cacacctttc cagctgttct tcagtcctca ggattgtatt 5460
ctcttagcag tgttgtgact gttccatcct caagcttggg cactcagacc tacatctgca 5520
atgtgaatca caaacccagc aacaccaagg ttgacaagaa agttgagccc aagtcttgtg 5580
acaagactca tacgtgtcca ccgtgcccag cacctgaact tcttggagga ccgtcagtct 5640
tcttgtttcc tccaaagcct aaggatacct tgatgatctc caggactcct gaagtcacat 5700
gtgtagttgt ggatgtgagc catgaagatc ctgaggtgaa gttcaactgg tatgtggatg 5760
gtgtggaagt gcacaatgcc aagacaaagc cgagagagga acagtacaac agcacgtaca 5820
gggttgtctc agttctcact gttctccatc aagattggtt gaatggcaaa gagtacaagt 5880
gcaaggtttc caacaaggcc ctcccagccc ccattgagaa gaccatttcc aaagcgaaag 5940
ggcaaccccg tgaaccacaa gtgtacacac ttcctccatc tcgcgatgaa ctgaccaaga 6000
accaggtcag cttgacttgc ctggtgaaag gcttctatcc ctctgacata gctgtagagt 6060
gggagagcaa tgggcaaccg gagaacaact acaagactac acctcccgtt ctcgattctg 6120
acggctcctt cttcctctac agcaagctca cagtggacaa gagcaggtgg caacaaggga 6180
atgtcttctc atgctccgtg atgcatgagg ctcttcacaa tcactacaca cagaagagtc 6240
tctccttgtc tccgggtaaa ggaggtggcg gatcaggtgg aggcggttca ggcggaggtg 6300
gatcttacaa gctggacata tcttaagagc tcaaagcaga atgctgagct aaaagaaagg 6360
ctttttccat tttcgagaga caatgagaaa agaagaagaa gaagaagaag aagaagaaga 6420
agaaaagagt aaataataaa gccccacagg aggcgaagtt cttgtagctc catgttatct 6480
aagttattga tattgtttgc cctatatttt atttctgtca ttgtgtatgt tttgttcagt 6540
ttcgatctcc ttgcaaaatg cagagattat gagatgaata aactaagtta tattattata 6600
cgtgttaata ttctcctcct ctctctagct agccttttgt tttctctttt tcttatttga 6660
ttttctttaa atcaatccat tttaggagag ggccagggag tgatccagca aaacatgaag 6720
attagaagaa acttccctct tttttttcct gaaaacaatt taacgtcgag atttatctct 6780
ttttgtaatg gaatcatttc tacagttatg acgctcatac agcattccca gaaagagaaa 6840
cagaagaaat atacaaactt tcattttgag agcagcacct cgtctattga ttgcagataa 6900
tatgcttctc atttgtattt ccttttgatt atttttgttt ctatcccttt gtttgagtca 6960
atctcaaata ttcggtcatt gttggtatga aaaatcaagc agttcatgtt aagagtcaat 7020
ttaaaattaa tatttttata tagagttgta tgtgaaatga tgttgtgatt tggtatatat 7080
ggataaagag cttgtcagtt cattttggtc tgattttttt ggtatccaaa taagaaacac 7140
aaaagggata tgtccctcta ctatcaaata ttagttataa gtattcatgt tatactattc 7200
gatattttct accccaatcg ttacctattt aaaagtattt acccctccat ctatcaaacc 7260
cctggaccca gctttcctat tacatgtggc ttcatcttaa gcccccaaac ctttttctta 7320
tttttgattt ttaaaggctc atcttaaaat ttattactca aattaatacc tcttaataac 7380
ccacctcaag gacccagtaa ttaaatatcc aattagctcc agtaattggg gttcatatta 7440
gctccagtct taaattttaa aggcgatgat cgtattcctc cacttggttc atttatactc 7500
aaagaatact caatgtcttt agtgtttaga taactttttg taaatcatat agattgtttt 7560
aacaaaaaac aattcaatag tagattttca catgaaagtt acataaaaat tctttaaaat 7620
tactttctca aaaaattgtt ccaaacatat tatcccacaa ttaaactcaa tctgtttttc 7680
gaaacctaaa tcaaaaccaa tccaactacc ttatataata tataatcaat acattgtaaa 7740
gaactgcatg ttcttttaaa ttttgggggc aaagttattc cgtacgttca cacatgtact 7800
aataggaggt aataaatgat atgtgaaaca atcgaggtgt aaacaagcta gcatgaattc 7860
tcgattaaaa atcccaatta tatttggtct aatttagttt ggtattgagt aaaacaaatt 7920
cgaaccaaac caaaatataa atatatagtt tttatatata tgcctttaag actttttata 7980
gaattttctt taaaaaatat ctaggtacat caacgaaaaa ttagtcaaac gactaaaata 8040
aataaatatc atgtgttatt aagaaaattc tcctataaga atattttaat agatcatatg 8100
tttgtaaaaa aaattaattt ttactaacac atatatttac ttatcaaaaa tttgacaaag 8160
taagattaaa ataatattca tctaacaaaa aaaaaaccag aaaatgctga aaacccggca 8220
aaaccgaacc aatccaaacc gatatagttg gtttggtttg attttgatat aaaccgaacc 8280
aactcggtcc atttgcaccc ctaatcataa tagctttaat atttcaagat attattaagt 8340
taacgttgtc aatatcctgg aaattttgca aaatgaatca agcctatatg gctgtaatat 8400
gaatttaaaa gcagctcgat gtggtggtaa tatgtaattt acttgattct aaaaaaatat 8460
cccaagtatt aataatttct gctaggaaga aggttagcta cgatttacag caaagccaga 8520
atacaaagaa ccataaagtg attgaagctc gaaatatacg aaggaacaaa tatttttaaa 8580
aaaatacgca atgacttgga acaaaagaaa gtgatatatt ttttgttctt aaacaagcat 8640
cccctctaaa gaatggcagt tttcctttgc atgtaactat tatgctccct tcgttacaaa 8700
aattttggac tactattggg aacttcttct gaaaatagtg gtaccgagtg tacttcaagt 8760
cagttggaaa tcaataaaat gattatttta tgaatatatt tcattgtgca agtagataga 8820
aattacatat gttacataac acacgaaata aacaaaaaaa cacaatccaa aacaaacacc 8880
ccaaacaaaa taacactata tatatcctcg tatgaggaga ggcacgttca gtgactcgac 8940
gattcccgag caaaaaaagt ctccccgtca cacatatagt gggtgacgca attatcttca 9000
aagtaatcct tctgttgact tgtcattgat aacatccagt cttcgtcagg attgcaaaga 9060
attatagaag ggattccacc ttttattttc ttcttttttc catatttagg gttgacagtg 9120
aaatcagact ggcaacctat taattgcttc cacaatggga cgaacttgaa ggggatgtcg 9180
tcgatgatat tataggtggc gtgttcatcg tagttggtga agtcgatggt cccgttccag 9240
tagttgtgtc gcccgagact tctagcccag gtggtctttc cggtacgagt tggtccgcag 9300
atgtagaggc tggggtgtct gaccccagtc cttccctcat cctggttaga tcggccatcc 9360
actcaaggtc agattgtgct tgatcgtagg agacaggatg tatgaaagtg taggcatcga 9420
tgcttacatg atataggtgc gtctctctcc agttgtgcag atcttcgtgg cagcggagat 9480
ctgattctgt gaagggcgac acgtactgct caggttgtgg aggaaataat ttgttggctg 9540
aatattccag ccattgaagc tttgttgccc attcatgagg gaactcttct ttgatcatgt 9600
caagatactc ctccttagac gttgcagtct ggataatagt tcgccatcgt gcgtcagatt 9660
tgcgaggaga caccttatga tctcggaaat ctcctctggt tttaatatct ccgtcctttg 9720
atatgtaatc aaggacttgt ttagagtttc tagctggctg gatattaggg tgatttcctt 9780
caaaatcgaa aaaagaagga tctctaatac aaggtttttt atcaagctgg ataagagcat 9840
gatagtgggt agtgccatct tgatgaagct cagaagcaac accaaggaag aaaataagaa 9900
aaggtgtgag tttctcccag agaaactgga ataaatcatc tctttgagat gagcacttgg 9960
ggtaggtaag gaaaacatat ttagattgga gtctgaagtt cttgctagca gaaggcatgt 10020
tgttgtgact ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag 10080
gcaagggcat tttggtaatt taagtagtta gtggaaaatg acgtcattta cttaaagacg 10140
aagtcttgcg acaagggggg cccacgccga attttaatat taccggcgtg gccccacctt 10200
atcgcgagtg ctttagcacg agcggtccag atttaaagta gaaaagttcc cgcccactag 10260
ggttaaaggt gttcacacta taaaagcata tacgatgtga tggtatttga tggagcgtat 10320
attgtatcag gtatttccgt cggatacgaa ttattcgtac ggccggaccg gtcccctagg 10380
ccggccaatt cgagatcggc cgcggctgag tggctccttc aatcgttgcg gttctgtcag 10440
ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta 10500
attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga 10560
caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt 10620
taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg 10680
ttccccagat ctggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc 10740
cgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag 10800
aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag 10860
caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat 10920
caggggtatg ttgggtttca cgtctggcct ccggagactg tcatacgcgt aaaaaggccg 10980
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 11040
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 11100
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 11160
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 11220
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 11280
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 11340
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 11400
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 11460
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 11520
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 11580
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 11640
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 11700
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgca gttgccatgt 11760
tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac 11820
caccccgtca gtagctgaac aggagggaca gctgatagac acagaagcca ctggagcacc 11880
tcaaaaacac catcatacac taaatcagta agttggcagc atcacccata attgtggttt 11940
caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc 12000
tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt 12060
ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg 12120
gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa 12180
gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta 12240
tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag 12300
gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg 12360
catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag 12420
tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc 12480
tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc 12540
gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa 12600
gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa 12660
gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc 12720
aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt 12780
gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt 12840
tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat 12900
gaattgtttt agtacctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga 12960
cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa 13020
ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca 13080
gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc 13140
aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca 13200
aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc 13260
ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg 13320
cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga 13380
cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg 13440
tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac 13500
tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc 13560
caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt 13620
gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc 13680
tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat 13740
tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga 13800
tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc 13860
gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta 13920
ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc 13980
cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg 14040
tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc 14100
tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg 14160
acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt 14220
ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga 14280
agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct 14340
ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagccag 14400
cgctttactg gcatttcagg aacaagcggg cactgctcga cgcacttgct tcgctcagta 14460
tcgctcggga cgcacggcgc gctctacgaa ctgccgataa acagaggatt aaaattgaca 14520
attcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg 14580
ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg 14640
ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca 14700
aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 14760
gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 14820
aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat 14880
ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 14940
cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 15000
cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 15060
gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 15120
gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct 15180
ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg 15240
agcgtcgcaa aggcgctcgg tcttgccttg ctcgtcgaga tctggggtcg atcagccggg 15300
gatgcatcag gccgacagtc ggaacttcgg gtccccgacc tgtaccattc ggtgagcaat 15360
ggatagggga gttgatatcg tcaacgttca cttctaaaga aatagcgcca ctcagcttcc 15420
tcagcggctt tatccagcga tttcctatta tgtcggcata gttctcaaga tcgacagcct 15480
gtcacggtta agcgagaaat gaataagaag gctgataatt cggatctctg cgagggagat 15540
gatatttgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc 15600
gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta 15660
acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg 15720
ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg 15780
ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat 15840
tgcggacgtt tttaatgtac tggggtggtt tttcttttca ccagtgagac gggcaacagc 15900
tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 15960
cccagcaggc gaaaatcctg tttgatggtg gttccgaaat cggcaaaatc ccttataaat 16020
caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 16080
taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 16140
tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 16200
ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 16260
gaaaggaagg gaagaaagcg aaaggagcgg gcgccattca ggctgcgcaa ctgttgggaa 16320
ggg 16323
<210> 74
<211> 15495
<212> DNA
<213> 人工序列
<220>
<223> 编码免疫球蛋白G变体的双元载体序列
<400> 74
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt ttgcctcaag 3480
agtatattac tcattttgtt tctgaacaag cccctaccgt tggtgaagct ggcggttccg 3540
gtggaagcct tccacaagaa tgggttcagt acttttacca agaggctgcc ccagctcaat 3600
cagtggccgg gggatctggt ggcagccttc ctcaagagtt cgtttcttgg tttgcttcca 3660
atgccttcac agtgcagtct gaggctggag gctccggtgg atcagcattg ttgcactacg 3720
tagacccaga caccaatcgt aaccttggag aaggtggatc tggaggttct gcgttgttgc 3780
gttttgtgaa tccagacact ggaagagtgc ttttcgaggg agggtctgga ggttcccttt 3840
tgttgagata cagaaacact cttactggac aattattgtt tgagggagga tctggaggtt 3900
cagtgtcttg ggtttctagg ttttatcagt tgaagcctgg cggctctggt ggatcagact 3960
cctgggtgaa tcagttttac actcttgcac ctggaggctc tggtggctct gtctcttggg 4020
ttccaagatt gtaccaatta gcttcaacta gtggtggatc aggaggttct ggtggttctg 4080
gaggttcagg atccgatgtt cagcttcttg agtctggagg tggtcttgtg caacctggag 4140
gttccttgag actctcctgt gcagcttcag ggtttgactt cagtaggtac tggatgagtt 4200
gggttcgtca agctcctggg aaaggactag aatggattgg agagatcaat ccagattcaa 4260
gtaccatcaa ctatactcca tctctgaagg atcgcttcac catttccaga gacaatgcca 4320
agaacacgtt gtatcttcag atgaacagct tgaggactga agacacagcc ttgtactact 4380
gcacaagaca gggctatggc tacaactact ggggtcaagg caccactgtc acagtgtctt 4440
cagctagcac caaaggtcca tcggtctttc cactggcacc ttcttccaag agtacttctg 4500
gaggcacagc tgcactgggt tgtcttgtca aggactactt tccagaacct gttacggttt 4560
cgtggaactc aggtgctctg accagtggag tgcacacctt tccagctgtt cttcagtcct 4620
caggattgta ttctcttagc agtgttgtga ctgttccatc ctcaagcttg ggcactcaga 4680
cctacatctg caatgtgaat cacaaaccca gcaacaccaa ggttgacaag aaagttgagc 4740
ccaagtcttg tgacaagact catacgtgtc caccgtgccc agcacctgaa cttcttggag 4800
gaccgtcagt cttcttgttt cctccaaagc ctaaggatac cttgatgatc tccaggactc 4860
ctgaagtcac atgtgtagtt gtggatgtga gccatgaaga tcctgaggtg aagttcaact 4920
ggtatgtgga tggtgtggaa gtgcacaatg ccaagacaaa gccgagagag gaacagtaca 4980
acagcacgta cagggttgtc tcagttctca ctgttctcca tcaagattgg ttgaatggca 5040
aagagtacaa gtgcaaggtt tccaacaagg ccctcccagc ccccattgag aagaccattt 5100
ccaaagcgaa agggcaaccc cgtgaaccac aagtgtacac acttcctcca tctcgcgatg 5160
aactgaccaa gaaccaggtc agcttgactt gcctggtgaa aggcttctat ccctctgaca 5220
tagctgtaga gtgggagagc aatgggcaac cggagaacaa ctacaagact acacctcccg 5280
ttctcgattc tgacggctcc ttcttcctct acagcaagct cacagtggac aagagcaggt 5340
ggcaacaagg gaatgtcttc tcatgctccg tgatgcatga ggctcttcac aatcactaca 5400
cacagaagag tctctccttg tctccgggta aaggaggtgg cggatcaggt ggaggcggtt 5460
caggcggagg tggatcatac aagctggaca tatcttaaga gctcaaagca gaatgctgag 5520
ctaaaagaaa ggctttttcc attttcgaga gacaatgaga aaagaagaag aagaagaaga 5580
agaagaagaa gaagaaaaga gtaaataata aagccccaca ggaggcgaag ttcttgtagc 5640
tccatgttat ctaagttatt gatattgttt gccctatatt ttatttctgt cattgtgtat 5700
gttttgttca gtttcgatct ccttgcaaaa tgcagagatt atgagatgaa taaactaagt 5760
tatattatta tacgtgttaa tattctcctc ctctctctag ctagcctttt gttttctctt 5820
tttcttattt gattttcttt aaatcaatcc attttaggag agggccaggg agtgatccag 5880
caaaacatga agattagaag aaacttccct cttttttttc ctgaaaacaa tttaacgtcg 5940
agatttatct ctttttgtaa tggaatcatt tctacagtta tgacgctcat acagcattcc 6000
cagaaagaga aacagaagaa atatacaaac tttcattttg agagcagcac ctcgtctatt 6060
gattgcagat aatatgcttc tcatttgtat ttccttttga ttatttttgt ttctatccct 6120
ttgtttgagt caatctcaaa tattcggtca ttgttggtat gaaaaatcaa gcagttcatg 6180
ttaagagtca atttaaaatt aatattttta tatagagttg tatgtgaaat gatgttgtga 6240
tttggtatat atggataaag agcttgtcag ttcattttgg tctgattttt ttggtatcca 6300
aataagaaac acaaaaggga tatgtccctc tactatcaaa tattagttat aagtattcat 6360
gttatactat tcgatatttt ctaccccaat cgttacctat ttaaaagtat ttacccctcc 6420
atctatcaaa cccctggacc cagctttcct attacatgtg gcttcatctt aagcccccaa 6480
acctttttct tatttttgat ttttaaaggc tcatcttaaa atttattact caaattaata 6540
cctcttaata acccacctca aggacccagt aattaaatat ccaattagct ccagtaattg 6600
gggttcatat tagctccagt cttaaatttt aaaggcgatg atcgtattcc tccacttggt 6660
tcatttatac tcaaagaata ctcaatgtct ttagtgttta gataactttt tgtaaatcat 6720
atagattgtt ttaacaaaaa acaattcaat agtagatttt cacatgaaag ttacataaaa 6780
attctttaaa attactttct caaaaaattg ttccaaacat attatcccac aattaaactc 6840
aatctgtttt tcgaaaccta aatcaaaacc aatccaacta ccttatataa tatataatca 6900
atacattgta aagaactgca tgttctttta aattttgggg gcaaagttat tccgtacgtt 6960
cacacatgta ctaataggag gtaataaatg atatgtgaaa caatcgaggt gtaaacaagc 7020
tagcatgaat tctcgattaa aaatcccaat tatatttggt ctaatttagt ttggtattga 7080
gtaaaacaaa ttcgaaccaa accaaaatat aaatatatag tttttatata tatgccttta 7140
agacttttta tagaattttc tttaaaaaat atctaggtac atcaacgaaa aattagtcaa 7200
acgactaaaa taaataaata tcatgtgtta ttaagaaaat tctcctataa gaatatttta 7260
atagatcata tgtttgtaaa aaaaattaat ttttactaac acatatattt acttatcaaa 7320
aatttgacaa agtaagatta aaataatatt catctaacaa aaaaaaaacc agaaaatgct 7380
gaaaacccgg caaaaccgaa ccaatccaaa ccgatatagt tggtttggtt tgattttgat 7440
ataaaccgaa ccaactcggt ccatttgcac ccctaatcat aatagcttta atatttcaag 7500
atattattaa gttaacgttg tcaatatcct ggaaattttg caaaatgaat caagcctata 7560
tggctgtaat atgaatttaa aagcagctcg atgtggtggt aatatgtaat ttacttgatt 7620
ctaaaaaaat atcccaagta ttaataattt ctgctaggaa gaaggttagc tacgatttac 7680
agcaaagcca gaatacaaag aaccataaag tgattgaagc tcgaaatata cgaaggaaca 7740
aatattttta aaaaaatacg caatgacttg gaacaaaaga aagtgatata ttttttgttc 7800
ttaaacaagc atcccctcta aagaatggca gttttccttt gcatgtaact attatgctcc 7860
cttcgttaca aaaattttgg actactattg ggaacttctt ctgaaaatag tggtaccgag 7920
tgtacttcaa gtcagttgga aatcaataaa atgattattt tatgaatata tttcattgtg 7980
caagtagata gaaattacat atgttacata acacacgaaa taaacaaaaa aacacaatcc 8040
aaaacaaaca ccccaaacaa aataacacta tatatatcct cgtatgagga gaggcacgtt 8100
cagtgactcg acgattcccg agcaaaaaaa gtctccccgt cacacatata gtgggtgacg 8160
caattatctt caaagtaatc cttctgttga cttgtcattg ataacatcca gtcttcgtca 8220
ggattgcaaa gaattataga agggattcca ccttttattt tcttcttttt tccatattta 8280
gggttgacag tgaaatcaga ctggcaacct attaattgct tccacaatgg gacgaacttg 8340
aaggggatgt cgtcgatgat attataggtg gcgtgttcat cgtagttggt gaagtcgatg 8400
gtcccgttcc agtagttgtg tcgcccgaga cttctagccc aggtggtctt tccggtacga 8460
gttggtccgc agatgtagag gctggggtgt ctgaccccag tccttccctc atcctggtta 8520
gatcggccat ccactcaagg tcagattgtg cttgatcgta ggagacagga tgtatgaaag 8580
tgtaggcatc gatgcttaca tgatataggt gcgtctctct ccagttgtgc agatcttcgt 8640
ggcagcggag atctgattct gtgaagggcg acacgtactg ctcaggttgt ggaggaaata 8700
atttgttggc tgaatattcc agccattgaa gctttgttgc ccattcatga gggaactctt 8760
ctttgatcat gtcaagatac tcctccttag acgttgcagt ctggataata gttcgccatc 8820
gtgcgtcaga tttgcgagga gacaccttat gatctcggaa atctcctctg gttttaatat 8880
ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt tctagctggc tggatattag 8940
ggtgatttcc ttcaaaatcg aaaaaagaag gatctctaat acaaggtttt ttatcaagct 9000
ggataagagc atgatagtgg gtagtgccat cttgatgaag ctcagaagca acaccaagga 9060
agaaaataag aaaaggtgtg agtttctccc agagaaactg gaataaatca tctctttgag 9120
atgagcactt ggggtaggta aggaaaacat atttagattg gagtctgaag ttcttgctag 9180
cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt 9240
ggaggcatgg aggcaagggc attttggtaa tttaagtagt tagtggaaaa tgacgtcatt 9300
tacttaaaga cgaagtcttg cgacaagggg ggcccacgcc gaattttaat attaccggcg 9360
tggccccacc ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaagtt 9420
cccgcccact agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt 9480
gatggagcgt atattgtatc aggtatttcc gtcggatacg aattattcgt acggccggac 9540
cggtccccta ggccggccaa ttcgagatcg gccgcggctg agtggctcct tcaatcgttg 9600
cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac 9660
gtgactccct taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact 9720
atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 9780
taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg 9840
ccaaccacag ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg 9900
cccgaaacga tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata 9960
cagcgccagc agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag 10020
gaggcccggc agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct 10080
cagaattacg atcaggggta tgttgggttt cacgtctggc ctccggagac tgtcatacgc 10140
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 10200
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 10260
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 10320
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 10380
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 10440
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 10500
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 10560
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 10620
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 10680
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 10740
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 10800
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 10860
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 10920
cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt 10980
gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag acacagaagc 11040
cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccca 11100
taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa 11160
ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga 11220
gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg 11280
aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac 11340
cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa 11400
aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg 11460
gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg 11520
cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt 11580
tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag 11640
tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac 11700
agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa 11760
aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg 11820
gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt 11880
gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag 11940
tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat 12000
gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat 12060
attttactgg atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca 12120
ggagcgcacc gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa 12180
gtatttgggc aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga 12240
gaaggacggc cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga 12300
caccaaggca ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg 12360
ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga 12420
actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat 12480
gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa 12540
gatcgagcgc gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt 12600
ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga 12660
cacgcgagga actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca 12720
ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat 12780
gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga 12840
cacggcccgc tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca 12900
aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct 12960
gcgggccgac gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc 13020
tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat 13080
caatggccgg tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat 13140
gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg 13200
cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt 13260
cgtgctgttt gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc 13320
gccgacggcc cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa 13380
gctggaaacc ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga 13440
gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt 13500
caatgatgac ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg 13560
ttcagcagcc agcgctttac tggcatttca ggaacaagcg ggcactgctc gacgcacttg 13620
cttcgctcag tatcgctcgg gacgcacggc gcgctctacg aactgccgat aaacagagga 13680
ttaaaattga caattcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt 13740
tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg 13800
gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc 13860
tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg 13920
tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc 13980
tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat 14040
cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc 14100
cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt 14160
tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac 14220
gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg 14280
gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc 14340
gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca 14400
accagcccgg tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcga gatctggggt 14460
cgatcagccg gggatgcatc aggccgacag tcggaacttc gggtccccga cctgtaccat 14520
tcggtgagca atggataggg gagttgatat cgtcaacgtt cacttctaaa gaaatagcgc 14580
cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca tagttctcaa 14640
gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa ttcggatctc 14700
tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta caatcaacat 14760
gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga 14820
atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt 14880
cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga 14940
gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac 15000
ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag 15060
acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc 15120
acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa atcggcaaaa 15180
tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca 15240
agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 15300
gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta 15360
aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 15420
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt caggctgcgc 15480
aactgttggg aaggg 15495
<210> 75
<211> 15404
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 75
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt gtaaacatga 3480
tagacccgtg gattgtgaac aacttcggag gctctggtgg aagccagaac gtcatcgacc 3540
cttggataag aaacaatttt ggaggatctg gtggcagcat caaccagatc gacccgtgga 3600
tatttcagaa ttttgggggt tctggtggat cacagtctcc acaaggggaa ttcaccatct 3660
caccaaacaa cactcctgga gatggtggct ccggaggttc ccaagcccct ggtggagagt 3720
tcactgtttc tccaagaaac gctcctggtg agggcggatc tggtgggagt cagtgcccct 3780
tgggtgaatt ttctatctct ccaagaaata ctcctggaga aggagggagt ggtggatcaa 3840
agatcatcgt ttgttgtgtg ccacctggag ggtccggtgg ctctaaggtt atctttgctg 3900
ctgttccacc cggaggctcc ggtggatcta aggttgtcgt agctttggtt cctccaacta 3960
gtggtggatc aggaggttct ggtggttctg gaggttcagg atccgatgtt cagcttcttg 4020
agtctggagg tggtcttgtg caacctggag gttccttgag actctcctgt gcagcttcag 4080
ggtttgactt cagtaggtac tggatgagtt gggttcgtca agctcctggg aaaggactag 4140
aatggattgg agagatcaat ccagattcaa gtaccatcaa ctatactcca tctctgaagg 4200
atcgcttcac catttccaga gacaatgcca agaacacgtt gtatcttcag atgaacagct 4260
tgaggactga agacacagcc ttgtactact gcacaagaca gggctatggc tacaactact 4320
ggggtcaagg caccactgtc acagtgtctt cagctagcac caaaggtcca tcggtctttc 4380
cactggcacc ttcttccaag agtacttctg gaggcacagc tgcactgggt tgtcttgtca 4440
aggactactt tccagaacct gttacggttt cgtggaactc aggtgctctg accagtggag 4500
tgcacacctt tccagctgtt cttcagtcct caggattgta ttctcttagc agtgttgtga 4560
ctgttccatc ctcaagcttg ggcactcaga cctacatctg caatgtgaat cacaaaccca 4620
gcaacaccaa ggttgacaag aaagttgagc ccaagtcttg tgacaagact catacgtgtc 4680
caccgtgccc agcacctgaa cttcttggag gaccgtcagt cttcttgttt cctccaaagc 4740
ctaaggatac cttgatgatc tccaggactc ctgaagtcac atgtgtagtt gtggatgtga 4800
gccatgaaga tcctgaggtg aagttcaact ggtatgtgga tggtgtggaa gtgcacaatg 4860
ccaagacaaa gccgagagag gaacagtaca acagcacgta cagggttgtc tcagttctca 4920
ctgttctcca tcaagattgg ttgaatggca aagagtacaa gtgcaaggtt tccaacaagg 4980
ccctcccagc ccccattgag aagaccattt ccaaagcgaa agggcaaccc cgtgaaccac 5040
aagtgtacac acttcctcca tctcgcgatg aactgaccaa gaaccaggtc agcttgactt 5100
gcctggtgaa aggcttctat ccctctgaca tagctgtaga gtgggagagc aatgggcaac 5160
cggagaacaa ctacaagact acacctcccg ttctcgattc tgacggctcc ttcttcctct 5220
acagcaagct cacagtggac aagagcaggt ggcaacaagg gaatgtcttc tcatgctccg 5280
tgatgcatga ggctcttcac aatcactaca cacagaagag tctctccttg tctccgggta 5340
aaggaggtgg cggatcaggt ggaggcggtt caggcggagg tggatctaca agctggacat 5400
atcttaagag ctcaaagcag aatgctgagc taaaagaaag gctttttcca ttttcgagag 5460
acaatgagaa aagaagaaga agaagaagaa gaagaagaag aagaaaagag taaataataa 5520
agccccacag gaggcgaagt tcttgtagct ccatgttatc taagttattg atattgtttg 5580
ccctatattt tatttctgtc attgtgtatg ttttgttcag tttcgatctc cttgcaaaat 5640
gcagagatta tgagatgaat aaactaagtt atattattat acgtgttaat attctcctcc 5700
tctctctagc tagccttttg ttttctcttt ttcttatttg attttcttta aatcaatcca 5760
ttttaggaga gggccaggga gtgatccagc aaaacatgaa gattagaaga aacttccctc 5820
ttttttttcc tgaaaacaat ttaacgtcga gatttatctc tttttgtaat ggaatcattt 5880
ctacagttat gacgctcata cagcattccc agaaagagaa acagaagaaa tatacaaact 5940
ttcattttga gagcagcacc tcgtctattg attgcagata atatgcttct catttgtatt 6000
tccttttgat tatttttgtt tctatccctt tgtttgagtc aatctcaaat attcggtcat 6060
tgttggtatg aaaaatcaag cagttcatgt taagagtcaa tttaaaatta atatttttat 6120
atagagttgt atgtgaaatg atgttgtgat ttggtatata tggataaaga gcttgtcagt 6180
tcattttggt ctgatttttt tggtatccaa ataagaaaca caaaagggat atgtccctct 6240
actatcaaat attagttata agtattcatg ttatactatt cgatattttc taccccaatc 6300
gttacctatt taaaagtatt tacccctcca tctatcaaac ccctggaccc agctttccta 6360
ttacatgtgg cttcatctta agcccccaaa cctttttctt atttttgatt tttaaaggct 6420
catcttaaaa tttattactc aaattaatac ctcttaataa cccacctcaa ggacccagta 6480
attaaatatc caattagctc cagtaattgg ggttcatatt agctccagtc ttaaatttta 6540
aaggcgatga tcgtattcct ccacttggtt catttatact caaagaatac tcaatgtctt 6600
tagtgtttag ataacttttt gtaaatcata tagattgttt taacaaaaaa caattcaata 6660
gtagattttc acatgaaagt tacataaaaa ttctttaaaa ttactttctc aaaaaattgt 6720
tccaaacata ttatcccaca attaaactca atctgttttt cgaaacctaa atcaaaacca 6780
atccaactac cttatataat atataatcaa tacattgtaa agaactgcat gttcttttaa 6840
attttggggg caaagttatt ccgtacgttc acacatgtac taataggagg taataaatga 6900
tatgtgaaac aatcgaggtg taaacaagct agcatgaatt ctcgattaaa aatcccaatt 6960
atatttggtc taatttagtt tggtattgag taaaacaaat tcgaaccaaa ccaaaatata 7020
aatatatagt ttttatatat atgcctttaa gactttttat agaattttct ttaaaaaata 7080
tctaggtaca tcaacgaaaa attagtcaaa cgactaaaat aaataaatat catgtgttat 7140
taagaaaatt ctcctataag aatattttaa tagatcatat gtttgtaaaa aaaattaatt 7200
tttactaaca catatattta cttatcaaaa atttgacaaa gtaagattaa aataatattc 7260
atctaacaaa aaaaaaacca gaaaatgctg aaaacccggc aaaaccgaac caatccaaac 7320
cgatatagtt ggtttggttt gattttgata taaaccgaac caactcggtc catttgcacc 7380
cctaatcata atagctttaa tatttcaaga tattattaag ttaacgttgt caatatcctg 7440
gaaattttgc aaaatgaatc aagcctatat ggctgtaata tgaatttaaa agcagctcga 7500
tgtggtggta atatgtaatt tacttgattc taaaaaaata tcccaagtat taataatttc 7560
tgctaggaag aaggttagct acgatttaca gcaaagccag aatacaaaga accataaagt 7620
gattgaagct cgaaatatac gaaggaacaa atatttttaa aaaaatacgc aatgacttgg 7680
aacaaaagaa agtgatatat tttttgttct taaacaagca tcccctctaa agaatggcag 7740
ttttcctttg catgtaacta ttatgctccc ttcgttacaa aaattttgga ctactattgg 7800
gaacttcttc tgaaaatagt ggtaccgagt gtacttcaag tcagttggaa atcaataaaa 7860
tgattatttt atgaatatat ttcattgtgc aagtagatag aaattacata tgttacataa 7920
cacacgaaat aaacaaaaaa acacaatcca aaacaaacac cccaaacaaa ataacactat 7980
atatatcctc gtatgaggag aggcacgttc agtgactcga cgattcccga gcaaaaaaag 8040
tctccccgtc acacatatag tgggtgacgc aattatcttc aaagtaatcc ttctgttgac 8100
ttgtcattga taacatccag tcttcgtcag gattgcaaag aattatagaa gggattccac 8160
cttttatttt cttctttttt ccatatttag ggttgacagt gaaatcagac tggcaaccta 8220
ttaattgctt ccacaatggg acgaacttga aggggatgtc gtcgatgata ttataggtgg 8280
cgtgttcatc gtagttggtg aagtcgatgg tcccgttcca gtagttgtgt cgcccgagac 8340
ttctagccca ggtggtcttt ccggtacgag ttggtccgca gatgtagagg ctggggtgtc 8400
tgaccccagt ccttccctca tcctggttag atcggccatc cactcaaggt cagattgtgc 8460
ttgatcgtag gagacaggat gtatgaaagt gtaggcatcg atgcttacat gatataggtg 8520
cgtctctctc cagttgtgca gatcttcgtg gcagcggaga tctgattctg tgaagggcga 8580
cacgtactgc tcaggttgtg gaggaaataa tttgttggct gaatattcca gccattgaag 8640
ctttgttgcc cattcatgag ggaactcttc tttgatcatg tcaagatact cctccttaga 8700
cgttgcagtc tggataatag ttcgccatcg tgcgtcagat ttgcgaggag acaccttatg 8760
atctcggaaa tctcctctgg ttttaatatc tccgtccttt gatatgtaat caaggacttg 8820
tttagagttt ctagctggct ggatattagg gtgatttcct tcaaaatcga aaaaagaagg 8880
atctctaata caaggttttt tatcaagctg gataagagca tgatagtggg tagtgccatc 8940
ttgatgaagc tcagaagcaa caccaaggaa gaaaataaga aaaggtgtga gtttctccca 9000
gagaaactgg aataaatcat ctctttgaga tgagcacttg gggtaggtaa ggaaaacata 9060
tttagattgg agtctgaagt tcttgctagc agaaggcatg ttgttgtgac tccgaggggt 9120
tgcctcaaac tctatcttat aaccggcgtg gaggcatgga ggcaagggca ttttggtaat 9180
ttaagtagtt agtggaaaat gacgtcattt acttaaagac gaagtcttgc gacaaggggg 9240
gcccacgccg aattttaata ttaccggcgt ggccccacct tatcgcgagt gctttagcac 9300
gagcggtcca gatttaaagt agaaaagttc ccgcccacta gggttaaagg tgttcacact 9360
ataaaagcat atacgatgtg atggtatttg atggagcgta tattgtatca ggtatttccg 9420
tcggatacga attattcgta cggccggacc ggtcccctag gccggccaat tcgagatcgg 9480
ccgcggctga gtggctcctt caatcgttgc ggttctgtca gttccaaacg taaaacggct 9540
tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag 9600
attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta 9660
aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt 9720
ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga tctggcgccg 9780
gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca 9840
ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg 9900
acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg 9960
atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc 10020
acgtctggcc tccggagact gtcatacgcg taaaaaggcc gcgttgctgg cgtttttcca 10080
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 10140
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 10200
tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 10260
gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 10320
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 10380
tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 10440
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 10500
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 10560
aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 10620
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 10680
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 10740
attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 10800
ctaaagtata tatgagtaaa cttggtctgc agttgccatg ttttacggca gtgagagcag 10860
agatagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa 10920
caggagggac agctgataga cacagaagcc actggagcac ctcaaaaaca ccatcataca 10980
ctaaatcagt aagttggcag catcacccat aattgtggtt tcaaaatcgg ctccgtcgat 11040
actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg 11100
ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt 11160
atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac 11220
cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc 11280
ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca 11340
gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg 11400
aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc 11460
tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc 11520
ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat 11580
cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga 11640
ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc 11700
cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc 11760
acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg 11820
atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga 11880
tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca 11940
agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag 12000
atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt 12060
tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg 12120
cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac 12180
ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa 12240
taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg 12300
acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat 12360
gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc 12420
ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc 12480
cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg 12540
gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga 12600
aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg 12660
aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg 12720
ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc 12780
aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac 12840
gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag 12900
caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac 12960
gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa 13020
tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg 13080
gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt 13140
tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa 13200
ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc 13260
agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg 13320
gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga 13380
ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag 13440
ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact ggcatttcag 13500
gaacaagcgg gcactgctcg acgcacttgc ttcgctcagt atcgctcggg acgcacggcg 13560
cgctctacga actgccgata aacagaggat taaaattgac aattcaatgg caaggactgc 13620
cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga 13680
tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg 13740
tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt 13800
taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa 13860
tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt 13920
cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc 13980
tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac 14040
tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc 14100
gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt 14160
caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc 14220
ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat 14280
agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgca aaggcgctcg 14340
gtcttgcctt gctcgtcgag atctggggtc gatcagccgg ggatgcatca ggccgacagt 14400
cggaacttcg ggtccccgac ctgtaccatt cggtgagcaa tggatagggg agttgatatc 14460
gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct ttatccagcg 14520
atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt aagcgagaaa 14580
tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga tcacaggcag 14640
caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa 14700
acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg 14760
ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg 14820
tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg 14880
tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta 14940
ctggggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct 15000
ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct 15060
gtttgatggt ggttccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat 15120
agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 15180
cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa 15240
atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 15300
ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 15360
gaaaggagcg ggcgccattc aggctgcgca actgttggga aggg 15404
<210> 76
<211> 15665
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 76
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat ggctaacaag cacctctcat 3420
tgtctctctt ccttgtgctc cttggtcttt ctgcttctct tgcttctggt actttgttcc 3480
cgcatgttat cgccgacgtg ggtggaagtg gagggtcaac aatgttccca catatcatcg 3540
tagacgttgg cggatctggt ggatctactt gttttccaca cgttatgtgc gatgttggag 3600
ggtctggcgg atctttgcag cttgggccac acttgaatcc cttccttagt gggggatctg 3660
gaggttctgc gccgcttgga ccagacctca atccgtactt gtctggaggt tccggtggat 3720
ctttggcttt gggacctggc ttaaatcctt acttagccgg aggttctgga ggttcttctt 3780
gggtttcaag gttctatcag ctcggaggat ctggaggctc ctcttgggtg aaccagtttt 3840
acacccttgg agggtctgga ggttcttcat gggttccaag attgtaccaa ctcggtggat 3900
ctggaggatc ttttgtatcc gagcaagccc ctactatggg tgatgccgct cttggcggtt 3960
ctggcggatc tttctaccaa gaggctgctc ctgctcagtc agatgttgct ttgggtggct 4020
ctggagggtc ttttgcttcc aacgctttca ctgttcaatc tgaggctctt cttggaggat 4080
ctggagggtc ttctccagcg cccgttggat ttccagattt cggaggctct ggagggtcca 4140
tcccagcccc acttggaact ccagacttcg gagggtctgg tggttcccgt gcggctcctc 4200
tcggataccc ggacttcact agtggtggat caggaggttc tggtggttct ggaggttcag 4260
gatccgatgt tcagcttctt gagtctggag gtggtcttgt gcaacctgga ggttccttga 4320
gactctcctg tgcagcttca gggtttgact tcagtaggta ctggatgagt tgggttcgtc 4380
aagctcctgg gaaaggacta gaatggattg gagagatcaa tccagattca agtaccatca 4440
actatactcc atctctgaag gatcgcttca ccatttccag agacaatgcc aagaacacgt 4500
tgtatcttca gatgaacagc ttgaggactg aagacacagc cttgtactac tgcacaagac 4560
agggctatgg ctacaactac tggggtcaag gcaccactgt cacagtgtct tcagctagca 4620
ccaaaggtcc atcggtcttt ccactggcac cttcttccaa gagtacttct ggaggcacag 4680
ctgcactggg ttgtcttgtc aaggactact ttccagaacc tgttacggtt tcgtggaact 4740
caggtgctct gaccagtgga gtgcacacct ttccagctgt tcttcagtcc tcaggattgt 4800
attctcttag cagtgttgtg actgttccat cctcaagctt gggcactcag acctacatct 4860
gcaatgtgaa tcacaaaccc agcaacacca aggttgacaa gaaagttgag cccaagtctt 4920
gtgacaagac tcatacgtgt ccaccgtgcc cagcacctga acttcttgga ggaccgtcag 4980
tcttcttgtt tcctccaaag cctaaggata ccttgatgat ctccaggact cctgaagtca 5040
catgtgtagt tgtggatgtg agccatgaag atcctgaggt gaagttcaac tggtatgtgg 5100
atggtgtgga agtgcacaat gccaagacaa agccgagaga ggaacagtac aacagcacgt 5160
acagggttgt ctcagttctc actgttctcc atcaagattg gttgaatggc aaagagtaca 5220
agtgcaaggt ttccaacaag gccctcccag cccccattga gaagaccatt tccaaagcga 5280
aagggcaacc ccgtgaacca caagtgtaca cacttcctcc atctcgcgat gaactgacca 5340
agaaccaggt cagcttgact tgcctggtga aaggcttcta tccctctgac atagctgtag 5400
agtgggagag caatgggcaa ccggagaaca actacaagac tacacctccc gttctcgatt 5460
ctgacggctc cttcttcctc tacagcaagc tcacagtgga caagagcagg tggcaacaag 5520
ggaatgtctt ctcatgctcc gtgatgcatg aggctcttca caatcactac acacagaaga 5580
gtctctcctt gtctccgggt aaaggaggtg gcggatcagg tggaggcggt tcaggcggag 5640
gtggatctac aagctggaca tatcttaaga gctcaaagca gaatgctgag ctaaaagaaa 5700
ggctttttcc attttcgaga gacaatgaga aaagaagaag aagaagaaga agaagaagaa 5760
gaagaaaaga gtaaataata aagccccaca ggaggcgaag ttcttgtagc tccatgttat 5820
ctaagttatt gatattgttt gccctatatt ttatttctgt cattgtgtat gttttgttca 5880
gtttcgatct ccttgcaaaa tgcagagatt atgagatgaa taaactaagt tatattatta 5940
tacgtgttaa tattctcctc ctctctctag ctagcctttt gttttctctt tttcttattt 6000
gattttcttt aaatcaatcc attttaggag agggccaggg agtgatccag caaaacatga 6060
agattagaag aaacttccct cttttttttc ctgaaaacaa tttaacgtcg agatttatct 6120
ctttttgtaa tggaatcatt tctacagtta tgacgctcat acagcattcc cagaaagaga 6180
aacagaagaa atatacaaac tttcattttg agagcagcac ctcgtctatt gattgcagat 6240
aatatgcttc tcatttgtat ttccttttga ttatttttgt ttctatccct ttgtttgagt 6300
caatctcaaa tattcggtca ttgttggtat gaaaaatcaa gcagttcatg ttaagagtca 6360
atttaaaatt aatattttta tatagagttg tatgtgaaat gatgttgtga tttggtatat 6420
atggataaag agcttgtcag ttcattttgg tctgattttt ttggtatcca aataagaaac 6480
acaaaaggga tatgtccctc tactatcaaa tattagttat aagtattcat gttatactat 6540
tcgatatttt ctaccccaat cgttacctat ttaaaagtat ttacccctcc atctatcaaa 6600
cccctggacc cagctttcct attacatgtg gcttcatctt aagcccccaa acctttttct 6660
tatttttgat ttttaaaggc tcatcttaaa atttattact caaattaata cctcttaata 6720
acccacctca aggacccagt aattaaatat ccaattagct ccagtaattg gggttcatat 6780
tagctccagt cttaaatttt aaaggcgatg atcgtattcc tccacttggt tcatttatac 6840
tcaaagaata ctcaatgtct ttagtgttta gataactttt tgtaaatcat atagattgtt 6900
ttaacaaaaa acaattcaat agtagatttt cacatgaaag ttacataaaa attctttaaa 6960
attactttct caaaaaattg ttccaaacat attatcccac aattaaactc aatctgtttt 7020
tcgaaaccta aatcaaaacc aatccaacta ccttatataa tatataatca atacattgta 7080
aagaactgca tgttctttta aattttgggg gcaaagttat tccgtacgtt cacacatgta 7140
ctaataggag gtaataaatg atatgtgaaa caatcgaggt gtaaacaagc tagcatgaat 7200
tctcgattaa aaatcccaat tatatttggt ctaatttagt ttggtattga gtaaaacaaa 7260
ttcgaaccaa accaaaatat aaatatatag tttttatata tatgccttta agacttttta 7320
tagaattttc tttaaaaaat atctaggtac atcaacgaaa aattagtcaa acgactaaaa 7380
taaataaata tcatgtgtta ttaagaaaat tctcctataa gaatatttta atagatcata 7440
tgtttgtaaa aaaaattaat ttttactaac acatatattt acttatcaaa aatttgacaa 7500
agtaagatta aaataatatt catctaacaa aaaaaaaacc agaaaatgct gaaaacccgg 7560
caaaaccgaa ccaatccaaa ccgatatagt tggtttggtt tgattttgat ataaaccgaa 7620
ccaactcggt ccatttgcac ccctaatcat aatagcttta atatttcaag atattattaa 7680
gttaacgttg tcaatatcct ggaaattttg caaaatgaat caagcctata tggctgtaat 7740
atgaatttaa aagcagctcg atgtggtggt aatatgtaat ttacttgatt ctaaaaaaat 7800
atcccaagta ttaataattt ctgctaggaa gaaggttagc tacgatttac agcaaagcca 7860
gaatacaaag aaccataaag tgattgaagc tcgaaatata cgaaggaaca aatattttta 7920
aaaaaatacg caatgacttg gaacaaaaga aagtgatata ttttttgttc ttaaacaagc 7980
atcccctcta aagaatggca gttttccttt gcatgtaact attatgctcc cttcgttaca 8040
aaaattttgg actactattg ggaacttctt ctgaaaatag tggtaccgag tgtacttcaa 8100
gtcagttgga aatcaataaa atgattattt tatgaatata tttcattgtg caagtagata 8160
gaaattacat atgttacata acacacgaaa taaacaaaaa aacacaatcc aaaacaaaca 8220
ccccaaacaa aataacacta tatatatcct cgtatgagga gaggcacgtt cagtgactcg 8280
acgattcccg agcaaaaaaa gtctccccgt cacacatata gtgggtgacg caattatctt 8340
caaagtaatc cttctgttga cttgtcattg ataacatcca gtcttcgtca ggattgcaaa 8400
gaattataga agggattcca ccttttattt tcttcttttt tccatattta gggttgacag 8460
tgaaatcaga ctggcaacct attaattgct tccacaatgg gacgaacttg aaggggatgt 8520
cgtcgatgat attataggtg gcgtgttcat cgtagttggt gaagtcgatg gtcccgttcc 8580
agtagttgtg tcgcccgaga cttctagccc aggtggtctt tccggtacga gttggtccgc 8640
agatgtagag gctggggtgt ctgaccccag tccttccctc atcctggtta gatcggccat 8700
ccactcaagg tcagattgtg cttgatcgta ggagacagga tgtatgaaag tgtaggcatc 8760
gatgcttaca tgatataggt gcgtctctct ccagttgtgc agatcttcgt ggcagcggag 8820
atctgattct gtgaagggcg acacgtactg ctcaggttgt ggaggaaata atttgttggc 8880
tgaatattcc agccattgaa gctttgttgc ccattcatga gggaactctt ctttgatcat 8940
gtcaagatac tcctccttag acgttgcagt ctggataata gttcgccatc gtgcgtcaga 9000
tttgcgagga gacaccttat gatctcggaa atctcctctg gttttaatat ctccgtcctt 9060
tgatatgtaa tcaaggactt gtttagagtt tctagctggc tggatattag ggtgatttcc 9120
ttcaaaatcg aaaaaagaag gatctctaat acaaggtttt ttatcaagct ggataagagc 9180
atgatagtgg gtagtgccat cttgatgaag ctcagaagca acaccaagga agaaaataag 9240
aaaaggtgtg agtttctccc agagaaactg gaataaatca tctctttgag atgagcactt 9300
ggggtaggta aggaaaacat atttagattg gagtctgaag ttcttgctag cagaaggcat 9360
gttgttgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt ggaggcatgg 9420
aggcaagggc attttggtaa tttaagtagt tagtggaaaa tgacgtcatt tacttaaaga 9480
cgaagtcttg cgacaagggg ggcccacgcc gaattttaat attaccggcg tggccccacc 9540
ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaagtt cccgcccact 9600
agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt 9660
atattgtatc aggtatttcc gtcggatacg aattattcgt acggccggac cggtccccta 9720
ggccggccaa ttcgagatcg gccgcggctg agtggctcct tcaatcgttg cggttctgtc 9780
agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct 9840
taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt 9900
gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 9960
tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 10020
ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga 10080
tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc 10140
agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc 10200
agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg 10260
atcaggggta tgttgggttt cacgtctggc ctccggagac tgtcatacgc gtaaaaaggc 10320
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 10380
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 10440
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 10500
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 10560
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 10620
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 10680
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 10740
cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 10800
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 10860
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 10920
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 10980
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 11040
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg cagttgccat 11100
gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt gccgttacgc 11160
accaccccgt cagtagctga acaggaggga cagctgatag acacagaagc cactggagca 11220
cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccca taattgtggt 11280
ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa 11340
gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg 11400
ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa 11460
tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa 11520
aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc 11580
tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa 11640
aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac 11700
ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag 11760
agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc 11820
tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag 11880
ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag 11940
aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg 12000
aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg 12060
gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca 12120
ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat 12180
tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg 12240
atgaattgtt ttagtaccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc 12300
gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc 12360
aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc 12420
cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca 12480
ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg 12540
caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac 12600
gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc 12660
cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc 12720
gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg 12780
cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga 12840
actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag 12900
gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc 12960
ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc 13020
tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc 13080
attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac 13140
gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag 13200
ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg 13260
tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg 13320
tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac 13380
cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt 13440
gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc 13500
cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc 13560
ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc 13620
gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac 13680
ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc 13740
agcgctttac tggcatttca ggaacaagcg ggcactgctc gacgcacttg cttcgctcag 13800
tatcgctcgg gacgcacggc gcgctctacg aactgccgat aaacagagga ttaaaattga 13860
caattcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt tcgcggccga 13920
ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag 13980
ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa 14040
caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa 14100
acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca 14160
ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc 14220
atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg 14280
tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg 14340
gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg 14400
gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt 14460
ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt 14520
ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg 14580
tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcga gatctggggt cgatcagccg 14640
gggatgcatc aggccgacag tcggaacttc gggtccccga cctgtaccat tcggtgagca 14700
atggataggg gagttgatat cgtcaacgtt cacttctaaa gaaatagcgc cactcagctt 14760
cctcagcggc tttatccagc gatttcctat tatgtcggca tagttctcaa gatcgacagc 14820
ctgtcacggt taagcgagaa atgaataaga aggctgataa ttcggatctc tgcgagggag 14880
atgatatttg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc 14940
gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg 15000
taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga 15060
tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct 15120
ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac 15180
attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag acgggcaaca 15240
gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 15300
gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa atcggcaaaa tcccttataa 15360
atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 15420
attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 15480
actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa 15540
tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 15600
gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt caggctgcgc aactgttggg 15660
aaggg 15665
<210> 77
<211> 15131
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 77
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctagca ccaaaggtcc atcggtcttt ccactggcac 3840
cttcttccaa gagtacttct ggaggcacag ctgcactggg ttgtcttgtc aaggactact 3900
ttccagaacc tgttacggtt tcgtggaact caggtgctct gaccagtgga gtgcacacct 3960
ttccagctgt tcttcagtcc tcaggattgt attctcttag cagtgttgtg actgttccat 4020
cctcaagctt gggcactcag acctacatct gcaatgtgaa tcacaaaccc agcaacacca 4080
aggttgacaa gaaagttgag cccaagtctt gtgacaagac tcatacgtgt ccaccgtgcc 4140
cagcacctga acttcttgga ggaccgtcag tcttcttgtt tcctccaaag cctaaggata 4200
ccttgatgat ctccaggact cctgaagtca catgtgtagt tgtggatgtg agccatgaag 4260
atcctgaggt gaagttcaac tggtatgtgg atggtgtgga agtgcacaat gccaagacaa 4320
agccgagaga ggaacagtac aacagcacgt acagggttgt ctcagttctc actgttctcc 4380
atcaagattg gttgaatggc aaagagtaca agtgcaaggt ctccaacaaa gccctcccag 4440
cccccattga gaagaccatt tccaaagcga aagggcaacc ccgtgaacca caagtgtaca 4500
cacttcctcc atctcgcgat gaactgacca agaaccaggt cagcttgact tgcctggtga 4560
aaggcttcta tccctctgac atagctgtag agtgggagag caatgggcaa ccggagaaca 4620
actacaagac tacacctccc gttctcgatt ctgacggctc cttcttcctc tacagcaagc 4680
tcacagtgga caagagcagg tggcaacaag ggaatgtctt ctcatgctcc gtgatgcatg 4740
aggctcttca caatcactac acacagaaga gtctctcctt gtctccgggt aaaggaggtg 4800
gcggatcagg tggaggcggt tcaggcggag gtggatcctt gcctcaagag tatattactc 4860
attttgtttc tgaacaagcc cctaccgttg gtgaagctgg cggttccggt ggaagccttc 4920
cacaagaatg ggttcagtac ttttaccaag aggctgcccc agctcaatca gtggccgggg 4980
gatctggtgg cagccttcct caagagttcg tttcttggtt tgcttccaat gccttcacag 5040
tgcagtctga ggctggaggc tccggtggat cagcattgtt gcactacgta gacccagaca 5100
ccaatcgtaa ccttggagaa ggtggatctg gaggttctgc gttgttgcgt tttgtgaatc 5160
cagacactgg aagagtgctt ttcgagggag ggtctggagg ttcccttttg ttgagataca 5220
gaaacactct tactggacaa ttattgtttg agggaggatc tggaggttca gtgtcttggg 5280
tttctaggtt ttatcagttg aagcctggcg gctctggtgg atcagactcc tgggtgaatc 5340
agttttacac tcttgcacct ggaggctctg gtggctctgt ctcttgggtt ccaagattgt 5400
accaattagc ttcaactagt tacaagctgg acatatctta agagctcaaa gcagaatgct 5460
gagctaaaag aaaggctttt tccattttcg agagacaatg agaaaagaag aagaagaaga 5520
agaagaagaa gaagaagaaa agagtaaata ataaagcccc acaggaggcg aagttcttgt 5580
agctccatgt tatctaagtt attgatattg tttgccctat attttatttc tgtcattgtg 5640
tatgttttgt tcagtttcga tctccttgca aaatgcagag attatgagat gaataaacta 5700
agttatatta ttatacgtgt taatattctc ctcctctctc tagctagcct tttgttttct 5760
ctttttctta tttgattttc tttaaatcaa tccattttag gagagggcca gggagtgatc 5820
cagcaaaaca tgaagattag aagaaacttc cctctttttt ttcctgaaaa caatttaacg 5880
tcgagattta tctctttttg taatggaatc atttctacag ttatgacggc tcactgagga 5940
aatatataga caaattaagt ttggttctat gagttctaat ttggacttaa gagttgtttg 6000
aaattctatt ttatagtgat gcttataatg tatttggact gttttctgct gtgtgtaaga 6060
ccttttggtc tgtgaactgg aaacatacat gaataaattt ctttgaattt actggaattt 6120
ttgcatcaac aaaagaaaaa ttgaagttac taacttgtaa atggaacaat tgtaatgtta 6180
aaggatataa atatcttaat atagtgcgat acgaatcaca cgaatgcaag actttctctc 6240
tctgctcccg ctcatgctct cggtgcatgt tagctaaata tacatcggtg catccatggc 6300
aggagcatga ggacggggat gaggaaggga gtgaggaggg ccaaaagaag tacacatagt 6360
ttcctttggg agcgaattct cgattaaaaa tcccaattat atttggtcta atttagtttg 6420
gtattgagta aaacaaattc gaaccaaacc aaaatataaa tatatagttt ttatatatat 6480
gcctttaaga ctttttatag aattttcttt aaaaaatatc tagaaatatt tgcgactctt 6540
ctggcatgta atatttcgtt aaatatgaag tgctccattt ttattaactt taaataattg 6600
gttgtacgat cactttctta tcaagtgtta ctaaaatgcg tcaatctctt tgttcttcca 6660
tattcatatg tcaaaatcta tcaaaattct tatatatctt tttcgaattt gaagtgaaat 6720
ttcgataatt taaaattaaa tagaacatat cattatttag gtatcatatt gatttttata 6780
cttaattact aaatttggtt aactttgaaa gtgtacatca acgaaaaatt agtcaaacga 6840
ctaaaataaa taaatatcat gtgttattaa gaaaattctc ctataagaat attttaatag 6900
atcatatgtt tgtaaaaaaa attaattttt actaacacat atatttactt atcaaaaatt 6960
tgacaaagta agattaaaat aatattcatc taacaaaaaa aaaaccagaa aatgctgaaa 7020
acccggcaaa accgaaccaa tccaaaccga tatagttggt ttggtttgat tttgatataa 7080
accgaaccaa ctcggtccat ttgcacccct aatcataata gctttaatat ttcaagatat 7140
tattaagtta acgttgtcaa tatcctggaa attttgcaaa atgaatcaag cctatatggc 7200
tgtaatatga atttaaaagc agctcgatgt ggtggtaata tgtaatttac ttgattctaa 7260
aaaaatatcc caagtattaa taatttctgc taggaagaag gttagctacg atttacagca 7320
aagccagaat acaaagaacc ataaagtgat tgaagctcga aatatacgaa ggaacaaata 7380
tttttaaaaa aatacgcaat gacttggaac aaaagaaagt gatatatttt ttgttcttaa 7440
acaagcatcc cctctaaaga atggcagttt tcctttgcat gtaactatta tgctcccttc 7500
gttacaaaaa ttttggacta ctattgggaa cttcttctga aaatagtggt accgagtgta 7560
cttcaagtca gttggaaatc aataaaatga ttattttatg aatatatttc attgtgcaag 7620
tagatagaaa ttacatatgt tacataacac acgaaataaa caaaaaaaca caatccaaaa 7680
caaacacccc aaacaaaata acactatata tatcctcgta tgaggagagg cacgttcagt 7740
gactcgacga ttcccgagca aaaaaagtct ccccgtcaca catatagtgg gtgacgcaat 7800
tatcttcaaa gtaatccttc tgttgacttg tcattgataa catccagtct tcgtcaggat 7860
tgcaaagaat tatagaaggg attccacctt ttattttctt cttttttcca tatttagggt 7920
tgacagtgaa atcagactgg caacctatta attgcttcca caatgggacg aacttgaagg 7980
ggatgtcgtc gatgatatta taggtggcgt gttcatcgta gttggtgaag tcgatggtcc 8040
cgttccagta gttgtgtcgc ccgagacttc tagcccaggt ggtctttccg gtacgagttg 8100
gtccgcagat gtagaggctg gggtgtctga ccccagtcct tccctcatcc tggttagatc 8160
ggccatccac tcaaggtcag attgtgcttg atcgtaggag acaggatgta tgaaagtgta 8220
ggcatcgatg cttacatgat ataggtgcgt ctctctccag ttgtgcagat cttcgtggca 8280
gcggagatct gattctgtga agggcgacac gtactgctca ggttgtggag gaaataattt 8340
gttggctgaa tattccagcc attgaagctt tgttgcccat tcatgaggga actcttcttt 8400
gatcatgtca agatactcct ccttagacgt tgcagtctgg ataatagttc gccatcgtgc 8460
gtcagatttg cgaggagaca ccttatgatc tcggaaatct cctctggttt taatatctcc 8520
gtcctttgat atgtaatcaa ggacttgttt agagtttcta gctggctgga tattagggtg 8580
atttccttca aaatcgaaaa aagaaggatc tctaatacaa ggttttttat caagctggat 8640
aagagcatga tagtgggtag tgccatcttg atgaagctca gaagcaacac caaggaagaa 8700
aataagaaaa ggtgtgagtt tctcccagag aaactggaat aaatcatctc tttgagatga 8760
gcacttgggg taggtaagga aaacatattt agattggagt ctgaagttct tgctagcaga 8820
aggcatgttg ttgtgactcc gaggggttgc ctcaaactct atcttataac cggcgtggag 8880
gcatggaggc aagggcattt tggtaattta agtagttagt ggaaaatgac gtcatttact 8940
taaagacgaa gtcttgcgac aaggggggcc cacgccgaat tttaatatta ccggcgtggc 9000
cccaccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga aaagttcccg 9060
cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg gtatttgatg 9120
gagcgtatat tgtatcaggt atttccgtcg gatacgaatt attcgtacgg ccggaccggt 9180
cccctaggcc ggccaattcg agatcggccg cggctgagtg gctccttcaa tcgttgcggt 9240
tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga 9300
ctcccttaat tctccgctca tgatcagatt gtcgtttccc gccttcagtt taaactatca 9360
gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat 9420
cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 9480
ccacagggtt ccccagatct ggcgccggcc agcgagacga gcaagattgg ccgccgcccg 9540
aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc 9600
gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg 9660
cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga 9720
attacgatca ggggtatgtt gggtttcacg tctggcctcc ggagactgtc atacgcgtaa 9780
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 9840
tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 9900
ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 9960
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 10020
ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 10080
ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 10140
gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 10200
agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 10260
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 10320
aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 10380
aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 10440
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 10500
aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgcagt 10560
tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg 10620
ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact 10680
ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat 10740
tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt 10800
gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc 10860
gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat 10920
aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct 10980
gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg 11040
aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac 11100
gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact 11160
ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct 11220
cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca 11280
tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc 11340
gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact 11400
gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa 11460
agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga 11520
aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt 11580
atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg 11640
agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt 11700
tactggatga attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag 11760
cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat 11820
ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag 11880
gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc 11940
aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca 12000
atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg 12060
atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt 12120
gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc 12180
gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag 12240
cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg 12300
cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc 12360
agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag 12420
ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg 12480
gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac 12540
aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg 12600
gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc 12660
ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat 12720
ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc 12780
ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc 12840
ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg 12900
ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg 12960
acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg 13020
gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag 13080
gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat 13140
gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca 13200
gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc 13260
gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa 13320
aattgacaat tcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc 13380
ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc 13440
gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt 13500
taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc 13560
cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa 13620
atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg 13680
cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca 13740
ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg 13800
aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg 13860
cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca 13920
agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg 13980
gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca 14040
gcccggtgag cgtcgcaaag gcgctcggtc ttgccttgct cgtcgagatc tggggtcgat 14100
cagccgggga tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg taccattcgg 14160
tgagcaatgg ataggggagt tgatatcgtc aacgttcact tctaaagaaa tagcgccact 14220
cagcttcctc agcggcttta tccagcgatt tcctattatg tcggcatagt tctcaagatc 14280
gacagcctgt cacggttaag cgagaaatga ataagaaggc tgataattcg gatctctgcg 14340
agggagatga tatttgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 14400
ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 14460
catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 14520
gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 14580
ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa 14640
taacacattg cggacgtttt taatgtactg gggtggtttt tcttttcacc agtgagacgg 14700
gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc 14760
tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc 14820
ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag 14880
tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 14940
tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc 15000
actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 15060
cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg ctgcgcaact 15120
gttgggaagg g 15131
<210> 78
<211> 15041
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 78
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctagca ccaaaggtcc atcggtcttt ccactggcac 3840
cttcttccaa gagtacttct ggaggcacag ctgcactggg ttgtcttgtc aaggactact 3900
ttccagaacc tgttacggtt tcgtggaact caggtgctct gaccagtgga gtgcacacct 3960
ttccagctgt tcttcagtcc tcaggattgt attctcttag cagtgttgtg actgttccat 4020
cctcaagctt gggcactcag acctacatct gcaatgtgaa tcacaaaccc agcaacacca 4080
aggttgacaa gaaagttgag cccaagtctt gtgacaagac tcatacgtgt ccaccgtgcc 4140
cagcacctga acttcttgga ggaccgtcag tcttcttgtt tcctccaaag cctaaggata 4200
ccttgatgat ctccaggact cctgaagtca catgtgtagt tgtggatgtg agccatgaag 4260
atcctgaggt gaagttcaac tggtatgtgg atggtgtgga agtgcacaat gccaagacaa 4320
agccgagaga ggaacagtac aacagcacgt acagggttgt ctcagttctc actgttctcc 4380
atcaagattg gttgaatggc aaagagtaca agtgcaaggt ctccaacaaa gccctcccag 4440
cccccattga gaagaccatt tccaaagcga aagggcaacc ccgtgaacca caagtgtaca 4500
cacttcctcc atctcgcgat gaactgacca agaaccaggt cagcttgact tgcctggtga 4560
aaggcttcta tccctctgac atagctgtag agtgggagag caatgggcaa ccggagaaca 4620
actacaagac tacacctccc gttctcgatt ctgacggctc cttcttcctc tacagcaagc 4680
tcacagtgga caagagcagg tggcaacaag ggaatgtctt ctcatgctcc gtgatgcatg 4740
aggctcttca caatcactac acacagaaga gtctctcctt gtctccgggt aaaggaggtg 4800
gcggatcagg tggaggcggt tcaggcggag gtggatccgt aaacatgata gacccgtgga 4860
ttgtgaacaa cttcggaggc tctggtggaa gccagaacgt catcgaccct tggataagaa 4920
acaattttgg aggatctggt ggcagcatca accagatcga cccgtggata tttcagaatt 4980
ttgggggttc tggtggatca cagtctccac aaggggaatt caccatctca ccaaacaaca 5040
ctcctggaga tggtggctcc ggaggttccc aagcccctgg tggagagttc actgtttctc 5100
caagaaacgc tcctggtgag ggcggatctg gtgggagtca gtgccccttg ggtgaatttt 5160
ctatctctcc aagaaatact cctggagaag gagggagtgg tggatcaaag atcatcgttt 5220
gttgtgtgcc acctggaggg tccggtggct ctaaggttat ctttgctgct gttccacccg 5280
gaggctccgg tggatctaag gttgtcgtag ctttggttcc tccaactagt tacaagctgg 5340
acatatctta agagctcaaa gcagaatgct gagctaaaag aaaggctttt tccattttcg 5400
agagacaatg agaaaagaag aagaagaaga agaagaagaa gaagaagaaa agagtaaata 5460
ataaagcccc acaggaggcg aagttcttgt agctccatgt tatctaagtt attgatattg 5520
tttgccctat attttatttc tgtcattgtg tatgttttgt tcagtttcga tctccttgca 5580
aaatgcagag attatgagat gaataaacta agttatatta ttatacgtgt taatattctc 5640
ctcctctctc tagctagcct tttgttttct ctttttctta tttgattttc tttaaatcaa 5700
tccattttag gagagggcca gggagtgatc cagcaaaaca tgaagattag aagaaacttc 5760
cctctttttt ttcctgaaaa caatttaacg tcgagattta tctctttttg taatggaatc 5820
atttctacag ttatgacggc tcactgagga aatatataga caaattaagt ttggttctat 5880
gagttctaat ttggacttaa gagttgtttg aaattctatt ttatagtgat gcttataatg 5940
tatttggact gttttctgct gtgtgtaaga ccttttggtc tgtgaactgg aaacatacat 6000
gaataaattt ctttgaattt actggaattt ttgcatcaac aaaagaaaaa ttgaagttac 6060
taacttgtaa atggaacaat tgtaatgtta aaggatataa atatcttaat atagtgcgat 6120
acgaatcaca cgaatgcaag actttctctc tctgctcccg ctcatgctct cggtgcatgt 6180
tagctaaata tacatcggtg catccatggc aggagcatga ggacggggat gaggaaggga 6240
gtgaggaggg ccaaaagaag tacacatagt ttcctttggg agcgaattct cgattaaaaa 6300
tcccaattat atttggtcta atttagtttg gtattgagta aaacaaattc gaaccaaacc 6360
aaaatataaa tatatagttt ttatatatat gcctttaaga ctttttatag aattttcttt 6420
aaaaaatatc tagaaatatt tgcgactctt ctggcatgta atatttcgtt aaatatgaag 6480
tgctccattt ttattaactt taaataattg gttgtacgat cactttctta tcaagtgtta 6540
ctaaaatgcg tcaatctctt tgttcttcca tattcatatg tcaaaatcta tcaaaattct 6600
tatatatctt tttcgaattt gaagtgaaat ttcgataatt taaaattaaa tagaacatat 6660
cattatttag gtatcatatt gatttttata cttaattact aaatttggtt aactttgaaa 6720
gtgtacatca acgaaaaatt agtcaaacga ctaaaataaa taaatatcat gtgttattaa 6780
gaaaattctc ctataagaat attttaatag atcatatgtt tgtaaaaaaa attaattttt 6840
actaacacat atatttactt atcaaaaatt tgacaaagta agattaaaat aatattcatc 6900
taacaaaaaa aaaaccagaa aatgctgaaa acccggcaaa accgaaccaa tccaaaccga 6960
tatagttggt ttggtttgat tttgatataa accgaaccaa ctcggtccat ttgcacccct 7020
aatcataata gctttaatat ttcaagatat tattaagtta acgttgtcaa tatcctggaa 7080
attttgcaaa atgaatcaag cctatatggc tgtaatatga atttaaaagc agctcgatgt 7140
ggtggtaata tgtaatttac ttgattctaa aaaaatatcc caagtattaa taatttctgc 7200
taggaagaag gttagctacg atttacagca aagccagaat acaaagaacc ataaagtgat 7260
tgaagctcga aatatacgaa ggaacaaata tttttaaaaa aatacgcaat gacttggaac 7320
aaaagaaagt gatatatttt ttgttcttaa acaagcatcc cctctaaaga atggcagttt 7380
tcctttgcat gtaactatta tgctcccttc gttacaaaaa ttttggacta ctattgggaa 7440
cttcttctga aaatagtggt accgagtgta cttcaagtca gttggaaatc aataaaatga 7500
ttattttatg aatatatttc attgtgcaag tagatagaaa ttacatatgt tacataacac 7560
acgaaataaa caaaaaaaca caatccaaaa caaacacccc aaacaaaata acactatata 7620
tatcctcgta tgaggagagg cacgttcagt gactcgacga ttcccgagca aaaaaagtct 7680
ccccgtcaca catatagtgg gtgacgcaat tatcttcaaa gtaatccttc tgttgacttg 7740
tcattgataa catccagtct tcgtcaggat tgcaaagaat tatagaaggg attccacctt 7800
ttattttctt cttttttcca tatttagggt tgacagtgaa atcagactgg caacctatta 7860
attgcttcca caatgggacg aacttgaagg ggatgtcgtc gatgatatta taggtggcgt 7920
gttcatcgta gttggtgaag tcgatggtcc cgttccagta gttgtgtcgc ccgagacttc 7980
tagcccaggt ggtctttccg gtacgagttg gtccgcagat gtagaggctg gggtgtctga 8040
ccccagtcct tccctcatcc tggttagatc ggccatccac tcaaggtcag attgtgcttg 8100
atcgtaggag acaggatgta tgaaagtgta ggcatcgatg cttacatgat ataggtgcgt 8160
ctctctccag ttgtgcagat cttcgtggca gcggagatct gattctgtga agggcgacac 8220
gtactgctca ggttgtggag gaaataattt gttggctgaa tattccagcc attgaagctt 8280
tgttgcccat tcatgaggga actcttcttt gatcatgtca agatactcct ccttagacgt 8340
tgcagtctgg ataatagttc gccatcgtgc gtcagatttg cgaggagaca ccttatgatc 8400
tcggaaatct cctctggttt taatatctcc gtcctttgat atgtaatcaa ggacttgttt 8460
agagtttcta gctggctgga tattagggtg atttccttca aaatcgaaaa aagaaggatc 8520
tctaatacaa ggttttttat caagctggat aagagcatga tagtgggtag tgccatcttg 8580
atgaagctca gaagcaacac caaggaagaa aataagaaaa ggtgtgagtt tctcccagag 8640
aaactggaat aaatcatctc tttgagatga gcacttgggg taggtaagga aaacatattt 8700
agattggagt ctgaagttct tgctagcaga aggcatgttg ttgtgactcc gaggggttgc 8760
ctcaaactct atcttataac cggcgtggag gcatggaggc aagggcattt tggtaattta 8820
agtagttagt ggaaaatgac gtcatttact taaagacgaa gtcttgcgac aaggggggcc 8880
cacgccgaat tttaatatta ccggcgtggc cccaccttat cgcgagtgct ttagcacgag 8940
cggtccagat ttaaagtaga aaagttcccg cccactaggg ttaaaggtgt tcacactata 9000
aaagcatata cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgtcg 9060
gatacgaatt attcgtacgg ccggaccggt cccctaggcc ggccaattcg agatcggccg 9120
cggctgagtg gctccttcaa tcgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt 9180
cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcagatt 9240
gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac 9300
ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta 9360
tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatct ggcgccggcc 9420
agcgagacga gcaagattgg ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt 9480
gcgcaggcaa attgcaccaa cgcatacagc gccagcagaa tgccatagtg ggcggtgacg 9540
tcgttcgagt gaaccagatc gcgcaggagg cccggcagca ccggcataat caggccgatg 9600
ccgacagcgt cgagcgcgac agtgctcaga attacgatca ggggtatgtt gggtttcacg 9660
tctggcctcc ggagactgtc atacgcgtaa aaaggccgcg ttgctggcgt ttttccatag 9720
gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 9780
gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 9840
tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 9900
ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 9960
ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 10020
tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 10080
tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 10140
ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 10200
aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 10260
ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 10320
tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 10380
atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 10440
aagtatatat gagtaaactt ggtctgcagt tgccatgttt tacggcagtg agagcagaga 10500
tagcgctgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag 10560
gagggacagc tgatagacac agaagccact ggagcacctc aaaaacacca tcatacacta 10620
aatcagtaag ttggcagcat cacccataat tgtggtttca aaatcggctc cgtcgatact 10680
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 10740
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 10800
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 10860
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 10920
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 10980
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 11040
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 11100
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 11160
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 11220
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 11280
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 11340
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 11400
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 11460
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 11520
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 11580
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagatg 11640
tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt 11700
ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag 11760
ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc 11820
attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa 11880
gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg 11940
tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc 12000
gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc 12060
tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct 12120
gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca 12180
ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa 12240
accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa 12300
cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg 12360
gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac 12420
aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg 12480
aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag 12540
gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag 12600
ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc 12660
ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa 12720
tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc 12780
caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc 12840
atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc 12900
tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat 12960
tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc 13020
agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc 13080
cttgtggggt cagttccggc tgggggttca gcagccagcg ctttactggc atttcaggaa 13140
caagcgggca ctgctcgacg cacttgcttc gctcagtatc gctcgggacg cacggcgcgc 13200
tctacgaact gccgataaac agaggattaa aattgacaat tcaatggcaa ggactgccag 13260
cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct ggggggatgg 13320
gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc 13380
gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa 13440
aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc 13500
tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag 13560
cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca 13620
agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg 13680
cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa 13740
atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa 13800
cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc 13860
ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga 13920
cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcgcaaag gcgctcggtc 13980
ttgccttgct cgtcgagatc tggggtcgat cagccgggga tgcatcaggc cgacagtcgg 14040
aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt tgatatcgtc 14100
aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta tccagcgatt 14160
tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag cgagaaatga 14220
ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca caggcagcaa 14280
cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 14340
cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 14400
tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 14460
ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 14520
tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 14580
gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc 14640
cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt 14700
tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc ccgagatagg 14760
gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 14820
caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc 14880
aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 14940
atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 15000
aggagcgggc gccattcagg ctgcgcaact gttgggaagg g 15041
<210> 79
<211> 15302
<212> DNA
<213> 人工序列
<220>
<223> 编码重组免疫复合物的载体序列
<400> 79
cgatcggtcg attcatagaa gattagattt ttcatagtat ttttttaaag taaaccttta 60
actacggtta ggacactttt aagttaaatt taatttgaac ccttaaatta atttttaaaa 120
tagataaata tcaatcatcc tgatatgctt ttgaaaaaat gaatgagaaa gatgattcaa 180
ttaaggccac attttaatca tgactaaaat aatatacagt ataatttcat atatatttgc 240
tttaaaaaaa aattgacaat ccattcgttt ctagcaataa atttcttcaa ccacaaatat 300
attaaagata actacggcat agaaacaaaa atctatgaag aatttttgta tacttcatat 360
gaaattaaaa aaaacttcat tgaacatcaa aataataata ataatcataa actcctcaat 420
atttatattc ctagcttctt gaattaaatt gtttacatat tcaacgatgt aaaaaattat 480
ttctctatct attttcctta tatcatgcat ggtttcacat atatcaaagg ataaaagcaa 540
tctatgtaaa ttatctcact ttattaagtt ttctatctga attattgaga acgtagattt 600
ctttttgcac tatcccccaa taattagcaa aacacaccta gactagattt gttttgctaa 660
cccaattgat attaattata tatgattaat atttatatgt atatggaatt ggttaataaa 720
atgcatctgg ttcatcaaag aattataaag acacgtgaca ttcatttagg ataagaaata 780
tggatgatct ctttctctta ttcagataat tagtaattac acataacaca caactttgat 840
gcccacatta tagtgattag catgtcacta tgtgtgcatc cttttatttc atacattaat 900
taacttggcc aatccagaag atggacaagt ctagggtcac attgcagggt actctagctt 960
actcgccttc tttttcgaag gtttgagtac cttcagggca tcctcttgat acattacttt 1020
ccacttcgat tggggcaagc tgtagcagtt cttgcttaga ccgaattgcc atctcacaga 1080
gatgctgaag agttcgcgac cctccagaaa cggtgatact aactcctcga aaccgaatac 1140
tataggtaca tccgatctgg tcgaaaccga aaaatcgaga tgctgcatag ttaaccgaat 1200
ctcccgtcca agatccaagg actctgtgca gtgaagcttc cgtcctgtcg tatctgagat 1260
atctcttaaa tacaactttc ccgaaacccc agctttcctt gaaaccaagg ggattatctt 1320
gattcgaatt cgtctcatcg ttatgtagcc gccactcagt ccaactcgga ctttcgtcag 1380
gaagtttgaa gggagaagtt gtacctcctg atcctccatc ccaacgttca ctgttagctt 1440
gttccctagc gtcgtttcct tgtatagctc gttccatgga ttgtaaatag taattgtaat 1500
gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacgctctcc aaatgaaatg 1560
aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta 1620
cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 1680
tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 1740
cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt 1800
ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg 1860
atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga 1920
tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt ctggcaattc cggttcgctt 1980
gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 2040
tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 2100
ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg 2160
cgccctgagt gcttgcggca gcgtgaagct ggcgcgccgc tctagcagaa ggcatgttgt 2220
tgtgactccg aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca 2280
agggcatttt ggtaatttaa gtagttagtg gaaaatgacg tcatttactt aaagacgaag 2340
tcttgcgaca aggggggccc acgccgaatt ttaatattac cggcgtggcc ccaccttatc 2400
gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aagttcccgc ccactagggt 2460
taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg agcgtatatt 2520
gtatcaggta tttccgtcgg atacgaatta ttcgtacgac cctcctgcag gtcaacatgg 2580
tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 2640
gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 2700
cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 2760
atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 2820
atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 2880
agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata 2940
tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat 3000
ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg 3060
aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag 3120
atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa 3180
aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg 3240
taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt 3300
catttcattt ggagaggacc tcgagaaaca aacaaaatca acaaatatag aaaataacgc 3360
atttccaatt ctttgaaatt tctgcaacat ctagaacaat gggatggtct tgcatcatac 3420
tctttcttgt tgcaactgct acaggtgtcc actctgatgt tcagcttctc gagtctggag 3480
gtggtcttgt gcaacctgga ggttccttga gactctcctg tgcagcttca gggtttgact 3540
tcagtaggta ctggatgagt tgggttcgtc aagctcctgg gaaaggacta gaatggattg 3600
gagagatcaa tccagattca agtaccatca actatactcc atctctgaag gatcgcttca 3660
ccatttccag agacaatgcc aagaacacgt tgtatcttca gatgaacagc ttgaggactg 3720
aagacacagc cttgtactac tgcacaagac agggctatgg ctacaactac tggggtcaag 3780
gcaccactgt cacagtgtct tcagctagca ccaaaggtcc atcggtcttt ccactggcac 3840
cttcttccaa gagtacttct ggaggcacag ctgcactggg ttgtcttgtc aaggactact 3900
ttccagaacc tgttacggtt tcgtggaact caggtgctct gaccagtgga gtgcacacct 3960
ttccagctgt tcttcagtcc tcaggattgt attctcttag cagtgttgtg actgttccat 4020
cctcaagctt gggcactcag acctacatct gcaatgtgaa tcacaaaccc agcaacacca 4080
aggttgacaa gaaagttgag cccaagtctt gtgacaagac tcatacgtgt ccaccgtgcc 4140
cagcacctga acttcttgga ggaccgtcag tcttcttgtt tcctccaaag cctaaggata 4200
ccttgatgat ctccaggact cctgaagtca catgtgtagt tgtggatgtg agccatgaag 4260
atcctgaggt gaagttcaac tggtatgtgg atggtgtgga agtgcacaat gccaagacaa 4320
agccgagaga ggaacagtac aacagcacgt acagggttgt ctcagttctc actgttctcc 4380
atcaagattg gttgaatggc aaagagtaca agtgcaaggt ctccaacaaa gccctcccag 4440
cccccattga gaagaccatt tccaaagcga aagggcaacc ccgtgaacca caagtgtaca 4500
cacttcctcc atctcgcgat gaactgacca agaaccaggt cagcttgact tgcctggtga 4560
aaggcttcta tccctctgac atagctgtag agtgggagag caatgggcaa ccggagaaca 4620
actacaagac tacacctccc gttctcgatt ctgacggctc cttcttcctc tacagcaagc 4680
tcacagtgga caagagcagg tggcaacaag ggaatgtctt ctcatgctcc gtgatgcatg 4740
aggctcttca caatcactac acacagaaga gtctctcctt gtctccgggt aaaggaggtg 4800
gcggatcagg tggaggcggt tcaggcggag gtggatccac tttgttcccg catgttatcg 4860
ccgacgtggg tggaagtgga gggtcaacaa tgttcccaca tatcatcgta gacgttggcg 4920
gatctggtgg atctacttgt tttccacacg ttatgtgcga tgttggaggg tctggcggat 4980
ctttgcagct tgggccacac ttgaatccct tccttagtgg gggatctgga ggttctgcgc 5040
cgcttggacc agacctcaat ccgtacttgt ctggaggttc cggtggatct ttggctttgg 5100
gacctggctt aaatccttac ttagccggag gttctggagg ttcttcttgg gtttcaaggt 5160
tctatcagct cggaggatct ggaggctcct cttgggtgaa ccagttttac acccttggag 5220
ggtctggagg ttcttcatgg gttccaagat tgtaccaact cggtggatct ggaggatctt 5280
ttgtatccga gcaagcccct actatgggtg atgccgctct tggcggttct ggcggatctt 5340
tctaccaaga ggctgctcct gctcagtcag atgttgcttt gggtggctct ggagggtctt 5400
ttgcttccaa cgctttcact gttcaatctg aggctcttct tggaggatct ggagggtctt 5460
ctccagcgcc cgttggattt ccagatttcg gaggctctgg agggtccatc ccagccccac 5520
ttggaactcc agacttcgga gggtctggtg gttcccgtgc ggctcctctc ggatacccgg 5580
acttcactag ttacaagctg gacatatctt aagagctcaa agcagaatgc tgagctaaaa 5640
gaaaggcttt ttccattttc gagagacaat gagaaaagaa gaagaagaag aagaagaaga 5700
agaagaagaa aagagtaaat aataaagccc cacaggaggc gaagttcttg tagctccatg 5760
ttatctaagt tattgatatt gtttgcccta tattttattt ctgtcattgt gtatgttttg 5820
ttcagtttcg atctccttgc aaaatgcaga gattatgaga tgaataaact aagttatatt 5880
attatacgtg ttaatattct cctcctctct ctagctagcc ttttgttttc tctttttctt 5940
atttgatttt ctttaaatca atccatttta ggagagggcc agggagtgat ccagcaaaac 6000
atgaagatta gaagaaactt ccctcttttt tttcctgaaa acaatttaac gtcgagattt 6060
atctcttttt gtaatggaat catttctaca gttatgacgg ctcactgagg aaatatatag 6120
acaaattaag tttggttcta tgagttctaa tttggactta agagttgttt gaaattctat 6180
tttatagtga tgcttataat gtatttggac tgttttctgc tgtgtgtaag accttttggt 6240
ctgtgaactg gaaacataca tgaataaatt tctttgaatt tactggaatt tttgcatcaa 6300
caaaagaaaa attgaagtta ctaacttgta aatggaacaa ttgtaatgtt aaaggatata 6360
aatatcttaa tatagtgcga tacgaatcac acgaatgcaa gactttctct ctctgctccc 6420
gctcatgctc tcggtgcatg ttagctaaat atacatcggt gcatccatgg caggagcatg 6480
aggacgggga tgaggaaggg agtgaggagg gccaaaagaa gtacacatag tttcctttgg 6540
gagcgaattc tcgattaaaa atcccaatta tatttggtct aatttagttt ggtattgagt 6600
aaaacaaatt cgaaccaaac caaaatataa atatatagtt tttatatata tgcctttaag 6660
actttttata gaattttctt taaaaaatat ctagaaatat ttgcgactct tctggcatgt 6720
aatatttcgt taaatatgaa gtgctccatt tttattaact ttaaataatt ggttgtacga 6780
tcactttctt atcaagtgtt actaaaatgc gtcaatctct ttgttcttcc atattcatat 6840
gtcaaaatct atcaaaattc ttatatatct ttttcgaatt tgaagtgaaa tttcgataat 6900
ttaaaattaa atagaacata tcattattta ggtatcatat tgatttttat acttaattac 6960
taaatttggt taactttgaa agtgtacatc aacgaaaaat tagtcaaacg actaaaataa 7020
ataaatatca tgtgttatta agaaaattct cctataagaa tattttaata gatcatatgt 7080
ttgtaaaaaa aattaatttt tactaacaca tatatttact tatcaaaaat ttgacaaagt 7140
aagattaaaa taatattcat ctaacaaaaa aaaaaccaga aaatgctgaa aacccggcaa 7200
aaccgaacca atccaaaccg atatagttgg tttggtttga ttttgatata aaccgaacca 7260
actcggtcca tttgcacccc taatcataat agctttaata tttcaagata ttattaagtt 7320
aacgttgtca atatcctgga aattttgcaa aatgaatcaa gcctatatgg ctgtaatatg 7380
aatttaaaag cagctcgatg tggtggtaat atgtaattta cttgattcta aaaaaatatc 7440
ccaagtatta ataatttctg ctaggaagaa ggttagctac gatttacagc aaagccagaa 7500
tacaaagaac cataaagtga ttgaagctcg aaatatacga aggaacaaat atttttaaaa 7560
aaatacgcaa tgacttggaa caaaagaaag tgatatattt tttgttctta aacaagcatc 7620
ccctctaaag aatggcagtt ttcctttgca tgtaactatt atgctccctt cgttacaaaa 7680
attttggact actattggga acttcttctg aaaatagtgg taccgagtgt acttcaagtc 7740
agttggaaat caataaaatg attattttat gaatatattt cattgtgcaa gtagatagaa 7800
attacatatg ttacataaca cacgaaataa acaaaaaaac acaatccaaa acaaacaccc 7860
caaacaaaat aacactatat atatcctcgt atgaggagag gcacgttcag tgactcgacg 7920
attcccgagc aaaaaaagtc tccccgtcac acatatagtg ggtgacgcaa ttatcttcaa 7980
agtaatcctt ctgttgactt gtcattgata acatccagtc ttcgtcagga ttgcaaagaa 8040
ttatagaagg gattccacct tttattttct tcttttttcc atatttaggg ttgacagtga 8100
aatcagactg gcaacctatt aattgcttcc acaatgggac gaacttgaag gggatgtcgt 8160
cgatgatatt ataggtggcg tgttcatcgt agttggtgaa gtcgatggtc ccgttccagt 8220
agttgtgtcg cccgagactt ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga 8280
tgtagaggct ggggtgtctg accccagtcc ttccctcatc ctggttagat cggccatcca 8340
ctcaaggtca gattgtgctt gatcgtagga gacaggatgt atgaaagtgt aggcatcgat 8400
gcttacatga tataggtgcg tctctctcca gttgtgcaga tcttcgtggc agcggagatc 8460
tgattctgtg aagggcgaca cgtactgctc aggttgtgga ggaaataatt tgttggctga 8520
atattccagc cattgaagct ttgttgccca ttcatgaggg aactcttctt tgatcatgtc 8580
aagatactcc tccttagacg ttgcagtctg gataatagtt cgccatcgtg cgtcagattt 8640
gcgaggagac accttatgat ctcggaaatc tcctctggtt ttaatatctc cgtcctttga 8700
tatgtaatca aggacttgtt tagagtttct agctggctgg atattagggt gatttccttc 8760
aaaatcgaaa aaagaaggat ctctaataca aggtttttta tcaagctgga taagagcatg 8820
atagtgggta gtgccatctt gatgaagctc agaagcaaca ccaaggaaga aaataagaaa 8880
aggtgtgagt ttctcccaga gaaactggaa taaatcatct ctttgagatg agcacttggg 8940
gtaggtaagg aaaacatatt tagattggag tctgaagttc ttgctagcag aaggcatgtt 9000
gttgtgactc cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg 9060
caagggcatt ttggtaattt aagtagttag tggaaaatga cgtcatttac ttaaagacga 9120
agtcttgcga caaggggggc ccacgccgaa ttttaatatt accggcgtgg ccccacctta 9180
tcgcgagtgc tttagcacga gcggtccaga tttaaagtag aaaagttccc gcccactagg 9240
gttaaaggtg ttcacactat aaaagcatat acgatgtgat ggtatttgat ggagcgtata 9300
ttgtatcagg tatttccgtc ggatacgaat tattcgtacg gccggaccgg tcccctaggc 9360
cggccaattc gagatcggcc gcggctgagt ggctccttca atcgttgcgg ttctgtcagt 9420
tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa 9480
ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac 9540
aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt 9600
aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 9660
tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc gaaacgatcc 9720
gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag cgccagcaga 9780
atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc 9840
accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc 9900
aggggtatgt tgggtttcac gtctggcctc cggagactgt catacgcgta aaaaggccgc 9960
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 10020
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 10080
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 10140
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 10200
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 10260
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 10320
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 10380
gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 10440
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 10500
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 10560
agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10620
agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10680
atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgcag ttgccatgtt 10740
ttacggcagt gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc 10800
accccgtcag tagctgaaca ggagggacag ctgatagaca cagaagccac tggagcacct 10860
caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccataa ttgtggtttc 10920
aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct 10980
gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta 11040
taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg 11100
ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag 11160
atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat 11220
atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg 11280
acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc 11340
atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt 11400
atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct 11460
ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg 11520
aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag 11580
acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag 11640
aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca 11700
aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg 11760
ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt 11820
ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg 11880
aattgtttta gtacctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac 11940
ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag 12000
gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag 12060
acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca 12120
ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa 12180
ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg 12240
gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc 12300
gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac 12360
agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt 12420
cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact 12480
atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc 12540
aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg 12600
ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct 12660
gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt 12720
ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat 12780
gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg 12840
atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat 12900
tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc 12960
gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt 13020
ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct 13080
ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga 13140
cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc 13200
cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa 13260
gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg 13320
gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccagc 13380
gctttactgg catttcagga acaagcgggc actgctcgac gcacttgctt cgctcagtat 13440
cgctcgggac gcacggcgcg ctctacgaac tgccgataaa cagaggatta aaattgacaa 13500
ttcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg 13560
gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg 13620
gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa 13680
ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg 13740
ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata 13800
ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc 13860
tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc 13920
acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc 13980
agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg 14040
agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg 14100
cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg 14160
gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga 14220
gcgtcgcaaa ggcgctcggt cttgccttgc tcgtcgagat ctggggtcga tcagccgggg 14280
atgcatcagg ccgacagtcg gaacttcggg tccccgacct gtaccattcg gtgagcaatg 14340
gataggggag ttgatatcgt caacgttcac ttctaaagaa atagcgccac tcagcttcct 14400
cagcggcttt atccagcgat ttcctattat gtcggcatag ttctcaagat cgacagcctg 14460
tcacggttaa gcgagaaatg aataagaagg ctgataattc ggatctctgc gagggagatg 14520
atatttgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg 14580
agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa 14640
catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg 14700
gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc 14760
tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt 14820
gcggacgttt ttaatgtact ggggtggttt ttcttttcac cagtgagacg ggcaacagct 14880
gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc 14940
ccagcaggcg aaaatcctgt ttgatggtgg ttccgaaatc ggcaaaatcc cttataaatc 15000
aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 15060
aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 15120
acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 15180
gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 15240
aaaggaaggg aagaaagcga aaggagcggg cgccattcag gctgcgcaac tgttgggaag 15300
gg 15302

Claims (28)

1.一种重组蛋白，其包含：
抗原单元；
包含IgG的CH2结构域和CH3结构域的Fc融合体单元；
IgG的可变重链(VH)结构域单元；
IgG的CH1结构域单元；和
IgG的轻链可变(VL)结构域单元，
其中：
抗原单元不是IgG的表位；
抗原单元在N-末端与IgG的VH结构域单元连接或在C-末端与IgG的CH3结构域连接；
IgG的CH1结构域单元与IgG的CH2结构域连接；并且
IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域连接。
2.根据权利要求1所述的重组蛋白，其中所述IgG是6D8抗体。
3.根据权利要求2所述的重组蛋白，其中所述Fc融合体包含置换突变E345R、E430G和S440Y。
4.一种重组蛋白，其包含：
抗原单元；
包含IgG的CH2结构域和CH3结构域的Fc融合体单元；
IgG的可变重链(VH)结构域单元；
IgG的CH1结构域单元；
IgG的轻链可变(VL)结构域单元；和
IgG的轻链恒定(CL)结构域单元，
其中：
IgG是6D8抗体；
Fc融合体包含置换突变E345R、E430G和S440Y；
抗原单元不是IgG的表位；
IgG的CH1结构域单元与IgG的CH2结构域连接；
IgG的VL结构域单元与IgG的CL结构域单元连接；
IgG的CL结构域单元与IgG的CH1结构域连接；并且
抗原单元与IgG的CH3结构域连接。
5.根据权利要求1-4中任一项所述的重组蛋白，其进一步包含表位标签单元，其中所述表位标签是IgG的表位。
6.根据权利要求5所述的重组蛋白，其中所述表位标签包含序列YKLDIS(SEQ IDNO.1)，并且表位标签单元与抗原单元连接。
7.根据权利要求6所述的重组蛋白，其中所述表位标签包含序列VYKLDISEA(SEQ IDNO.2)。
8.根据权利要求6所述的重组蛋白，其中所述表位标签由序列YKLDIS(SEQ ID NO.1)组成。
9.根据权利要求2所述的重组蛋白，其包含：
两个抗原单元；
两个Fc融合体单元；
两个IgG的CH1结构域单元；
两个IgG的VH结构域单元；和
两个IgG的轻链可变(VL)结构域单元，
其中在IgG的CH2结构域和CH1结构域的连接处形成的二硫键连接两个Fc融合体单元。
10.根据权利要求9所述的重组蛋白，其中每个IgG的VL结构域单元与IgG的VH结构域单元和IgG的CH1结构域连接。
11.根据权利要求10所述的重组蛋白，其中所述重组蛋白不包含IgG的任何轻链恒定(CL)结构域。
12.根据权利要求9-11中任一项所述的重组蛋白，其进一步包含两个表位标签单元，其中：
每个表位标签单元包含序列YKLDIS(SEQ ID NO.1)，
两个表位标签单元与两个Fc融合体单元在IgG的CH3结构域的C-末端处连接，并且
两个抗原单元与两个IgG的VH结构域单元连接。
13.根据权利要求9-11中任一项所述的重组蛋白，其进一步包含两个表位标签单元，其中：
每个表位标签单元包含序列YKLDIS(SEQ ID NO.1)，
两个表位标签单元与两个抗原单元连接，并且
两个抗原单元与两个Fc融合体单元在IgG的CH3结构域的C-末端处连接。
14.根据权利要求12或13所述的重组蛋白，其中所述表位标签包含序列VYKLDISEA(SEQID NO.2)。
15.根据权利要求12或13所述的重组蛋白，其中所述表位标签由序列YKLDIS(SEQ IDNO.1)组成。
16.一种重组蛋白，其包含：
两个抗原单元；
两个Fc融合体单元；
两个IgG的VH结构域单元；和
两个IgG的CH1结构域单元，
其中：
所述抗原不是IgG的表位；
两个抗原单元在N-末端与两个IgG的VH结构域单元连接；并且
两个IgG的CH1结构域单元与两个Fc融合体单元在IgG的CH2结构域处连接。
17.根据权利要求16所述的重组蛋白，其中所述重组蛋白不包含IgG的轻链恒定(CL)结构域，并且不包含IgG的轻链可变(VL)结构域。
18.根据权利要求1-17中任一项所述的重组蛋白，其中所述抗原包含登录号AMC13911.1的K301-T406。
19.根据权利要求1-17中任一项所述的重组蛋白，其中所述抗原包含至少一个选自SEQID NO.34-72的序列。
20.根据权利要求19所述的重组蛋白，其中所述抗原包含至少三个、至少六个、或至少九个选自SEQ ID NO.34-72的序列。
21.根据权利要求1-20中任一项所述的重组蛋白，其中所述重组蛋白在转基因植物中产生。
22.根据权利要求21所述的重组蛋白，其中所述转基因植物的木糖基转移酶和岩藻糖基转移酶被沉默。
23.一种免疫组合物，其包含：
至少一种根据权利要求1-22中任一项所述的重组蛋白，或
至少一种根据权利要求1和3-22中任一项所述的重组蛋白，其中所述抗体是6D8抗体。
24.一种生产根据权利要求1-22中任一项所述的重组蛋白的方法，所述方法包括：
将选自以下的双元载体引入第一农杆菌中：pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、pBYKEMd-HZE3、pBYKEMd-ZE3H、pBYKEMd-ZE3Hx、pBYKEMd-HVLZe、pBYKEMd2-HVL-Hx、pBYKEMd2-HVLZnt、pBYKEMd2-ZHVLnt、pBYKEMd2-ZHVLe、pBYKEMd2-ZHVLhx、pBYKEAM-N12MHd、pBYKEAM-NPHd、pBYKEAM-NSHd、pBYKEAM-NTHd、pBYKEHM-CPHd、pBYKEHM-CSHd、和pBYKEHM-CTHd；
用含有双元载体的第一农杆菌渗入植物部分以产生转化的植物部分；和
从所述转化的植物部分提取粗蛋白；和
纯化提取的粗蛋白。
25.根据权利要求24所述的方法，其中引入农杆菌中的双元载体选自：pBYR11eM-h6D8ZE3、pBYR11eMa-BAZE3-Hgp371、pBYR11eMa-BAZE3-H、和pBYKEMd-HZE3，所述方法进一步包括：
将pBYKEMd-6D8K引入第二农杆菌中；和
用含有pBYKEMd-6D8K的第二农杆菌渗入植物部分以产生转化的植物部分，
其中用含有pBYKEMd-6D8K的第二农杆菌和含有双元载体的第一农杆菌共渗入所述植物部分。
26.一种在受试者中诱导针对抗原的免疫应答的方法，所述方法包括向受试者施用至少一种选自根据权利要求1-22中任一项所述的重组蛋白的重组蛋白或根据权利要求23所述的免疫组合物。
27.根据权利要求26所述的方法，其中所述抗原来自寨卡病毒，所述方法包括向受试者施用根据权利要求18、21和22中任一项所述的重组蛋白或根据权利要求23所述的免疫组合物。
28.根据权利要求27所述的方法，其中所述抗原来自诺如病毒，所述方法包括向受试者施用根据权利要求19-22中任一项所述的重组蛋白或根据权利要求23所述的免疫组合物。
CN202180015476.0A 2020-02-21 2021-02-22 用于无佐剂诱导免疫应答的IgG变体 Pending CN115243701A (zh)

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