KR20230085124A - A functional cosmetic composition comprising fermentation product of melia toosendan and corydalis turtschaninovii - Google Patents

Abstract

The present invention relates to a functional cosmetic composition comprising a mixed fermented product of Melia toosendan and Corydalis turtschaninovii by Phellinus baumii Mycelium, and more specifically, It relates to a functional cosmetic composition for antioxidation, antibacterial, anti-aging, and improvement of skin elasticity, comprising a fermented product obtained by inoculating and culturing a mycelium culture solution of Phellinus linteus in a mixed dried powder of golden chinensis and corydalis. The mixed fermented product of Geumryeongja and Corydalis fermented with Phellinus Phellinus mycelium of the present invention is a natural product-derived composition without cytotoxicity, and has excellent antioxidant, antibacterial, skin aging prevention and skin elasticity improvement activities, so it is applied as a raw material composition for functional cosmetic compositions Therefore, it is very useful in the cosmetics industry.

Description

Functional cosmetic composition containing a mixed fermented product of gold and corydalis

The present invention is a mushroom mycelium ( Phellinus It relates to a functional cosmetic composition comprising a mixed fermented product of baumii Mycelium ( Melia toosendan ) and corydalis ( Corydalis turtschaninovii ). It relates to a functional cosmetic composition for anti-oxidation, anti-bacterial, anti-aging and improvement of skin elasticity comprising a fermented product obtained by inoculating and culturing a mixed dry powder of.

The skin is a part of the body that is directly exposed to the external environment. In daily life, it is exposed to ultraviolet rays from exposure to sunlight, air pollutants such as yellow dust or car exhaust, and various bacteria, which cause skin trouble and irritation, which causes normal skin aging. will accelerate Such skin aging causes skin pigmentation by reducing the elasticity of the skin in terms of appearance, forming wrinkles, or forming melasma and senile spots.

In general, various theories have been proposed for skin aging, such as somatic cell mutation, excessive generation of free radicals, autoimmune reaction, accumulation of toxic substances, errors generated during gene duplication, and transformation of dermal constituent proteins. As the theory that the cause of various diseases such as adult diseases is due to reactive oxygen species has been recognized, research to develop natural antioxidants that can control reactive oxygen species derived from oxygen or nitrogen is being actively conducted. there is. Reactive oxygen species, commonly known as harmful oxygen, are lipid peroxides such as peroxide anion radicals, hydrogen peroxide, hydroxy radicals, lipid peroxides, and free radicals generated from these, as triplet oxygen, which is the most stable form, is reduced. Although it occurs in normal conditions, antioxidant enzymes such as superoxide dismutase (SOD), catalase, and glutathion peroxidase (GPx) exist to suppress excessive generation of reactive oxygen species. However, when these reactive oxygen species are abnormally excessively generated by stress and external environmental factors and remain in the body without being eliminated, oxidative stress due to free radicals is applied to the living body, resulting in DNA, RNA, protein, It is speculated that it causes cell membrane and cellular structure damage, cancer, tissue damage, and aging.

In order to suppress the acceleration of various diseases and aging, which are rapidly increasing in modern times, research on antioxidants capable of controlling or removing these reactive oxygen species is being actively conducted. Representatively, studies on the efficacy of natural antioxidants such as tocopherol, ascorbic acid, carotenoids, flavonoids, and glutathione and their stabilization technology, as well as synthetic antioxidants such as butylated hydroxy toluene (BHT) and butylated hydroxy anisole (BHA) and other antioxidants are known, and research on many other antioxidants is continuously being conducted. Among these antioxidants, tocopherol has a relatively low antioxidant effect, and synthetic antioxidants such as BHA and BHT, which have excellent effects, are used in the pharmaceutical and food fields. With the development of secured derivatives and formulations in progress, there is an urgent need to develop a more stable and effective natural antioxidant material.

In order to solve this problem, many studies have been conducted on cosmetic compositions using extracts that are relatively low in toxicity and safe among phytochemicals that have recently been in the limelight and have effective effects on the skin, but obtained by a general extraction method. The components that have been used have a marginal point of insignificant effect, so the development of materials with maximized efficacy is required.

On the other hand, in the case of natural extracts through fermentation, sugar components present in natural products are used as carbon sources in the metabolism of microorganisms and converted into alcohols without using organic solvents harmful to the human body. Polyphenols, catechins, etc. are saccharified and exist as stable compounds, so the active ingredient content extraction rate is increased through fermentation, and excellent efficacy is expected by active ingredients activated by excluding sugar.

Recently, consumers' awareness of mushrooms has expanded not only to edible but also to medicinal and taste foods, and as it has been revealed that fruiting bodies and mycelial extracts or mycelial cultures of mushrooms are effective in preventing and treating various diseases, mushrooms are emerging as a new bio-industrial material. It has emerged as an important biological resource for the development of functional foods and medicines. About 20,000 species of mushrooms are known worldwide, and among them, about 2,000 species can be developed for edible use. About 992 species of mushrooms distributed in Korea have been recorded, among which edible mushrooms are about 100 species, poisonous mushrooms are about 50 species, and especially poisonous mushrooms are about 20 species. More than 20 types of mushrooms can be cultivated in Korea, and anti-cancer, cholesterol lowering, and blood sugar lowering have been proven.

KR 10-2010-0011449 A KR 10-2011-0096102 A KR 10-2011-0108687 A

The present invention was made to solve the problems of the prior art as described above, and the problem to be solved in the present invention is to obtain a fermented product obtained by fermenting a mixed dry powder of golden rice and corydalis as a mycelium of Phellinus linteus, derived from a non-cytotoxic natural product. It is intended to provide a functional cosmetic composition comprising

The present invention has been made to solve the above problems, and the present invention provides a functional cosmetic composition containing a mixed fermented product of Geumnyeongja (金鈴子) and Corydalis (玄胡索) by inoculation of Phellinus Phellinus mycelium.

The mixed fermented product of Geumnyeongja and Corydalis by inoculation of the Phellinus Phellinus mycelium is (1) drying and powdering the Geumnyeongja and Corydalis; (2) inoculating the mycelium culture solution of Phellinus Phellinus in a culture flask containing powdered Geumnyeongja and Corydalis; (3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) extracting with hot water and pulverizing.

The functional cosmetic composition preferably further includes a mixed fermented product of gold and corydalis by inoculation of cordyceps mycelium.

The mixed fermented product of the cordyceps sinensis mycelium inoculated with goldenrod and corydalis is: (1) drying and powdering the goldenrod and corydalis; (2) inoculating a culture solution of Cordyceps sinensis mycelium in a culture flask containing powdered geumnyeongja and corydalis; (3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) extracting with hot water and pulverizing.

The functionality is preferably selected from the group consisting of antioxidant, antibacterial, skin aging prevention and skin elasticity improvement.

The cosmetic composition is flexible lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, powder foundation, emulsion foundation, wax foundation, cleansing cream, cleansing foam, cleansing water, pack, spray, powder, pact, lip gloss , It is preferably a formulation selected from the group consisting of lipstick, shadow, shampoo and rinse.

The mixed fermented product of Geumryeongja and Corydalis fermented with Phellinus Phellinus mycelium of the present invention is a natural product-derived composition without cytotoxicity, and has excellent antioxidant, antibacterial, skin aging prevention and skin elasticity improvement activities, so it is applied as a raw material composition for functional cosmetic compositions Therefore, it is very useful in the cosmetics industry.

Hereinafter, the present invention will be described in detail.

As a result of repeated research to develop materials for functional cosmetic compositions using natural products, the inventors of the present invention inoculated the mycelium of Phellinus linteus in dry powders of golden brown and corydalis, and produced them according to the solid culture method. It was confirmed that the mixed fermented product of Geumnyeongja and Corydalis by the mycelium of Sanghwang mushroom obtained by culturing had excellent antioxidant, antibacterial, anti-aging and skin elasticity improvement effects, and the Phellinus linteus of the present invention It is proposed that a mixed fermented product of golden bell and corydalis by mycelium can be used as a raw material composition for a functional cosmetic composition.

Therefore, the present invention provides a functional cosmetic composition comprising a mixed fermented product of Geumnyeongja (金鈴子) and Corydalis (玄胡索) by inoculation of Phellinus Phellinus mycelium.

The mixed fermented product of Geumnyeongja and Corydalis by inoculation of the Phellinus Phellinus mycelium is (1) drying and powdering the Geumnyeongja and Corydalis; (2) inoculating the mycelium culture solution of Phellinus Phellinus in a culture flask containing powdered Geumnyeongja and Corydalis; (3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) extracting with hot water and pulverizing.

The situation mushroom is also called woody mud mushroom, and the cap has various shapes such as a diameter of 6 to 12 cm, a thickness of 2 to 10 cm, a semicircular shape, a flat shape, a rounded mountain shape, and a horseshoe shape. On the surface, short and dense dark brown hairs grow, disappear as they grow, and become cuticles. In the early stage of growth, it looks like a lump of mud, but after it grows up, it looks like a tongue sticking out from a tree stump, so it is also called tree tongue. Since it was known to have anticancer effects by Kiekawa et al. in Japan in 1968, many studies have been conducted. In addition to anticancer effects, it exhibits various pharmacological effects such as immunoregulatory effects, antioxidant effects, liver function improvement actions, hyperlipidemia improvement actions, and whitening effects. If it is decocted for medicinal use, it is characterized by being yellow or light yellow and clear, and having no taste or scent. It grows wild in Korea, Japan, Australia and North America.

The Geumnyeongja (金鈴子) refers to the fruit of the mulberry tree of the mulberry family, and the fruit is shaped like a small bell and becomes yellow when ripe, so it got the name Geumryeong (金鈴). As an oriental medicine, it has a peculiar smell, tastes bitter, is cold, and has a little poison. As for the efficacy, it is used for sore throat, abdominal pain, testicular pain, etc., as the liver qi gathers and does not disperse, and is also used to remove parasites and treat psoriasis dermatitis.

The corydalis (玄胡索) grows in a damp place at the foot of the mountain, the tuber is about 1cm in diameter, and the stem from here grows about 20cm. One bract-like leaf hangs at the bottom, and the branches diverge there. The leaves are alternate, the petioles are long, and they are divided into 3 pieces once or twice. The split piece is obovate, the upper part is deeply split or notched, and the edge has saw teeth, and the back side is pinkish white. Flowers bloom in April, light reddish purple, and 5 to 10 flowers run in raceme. The corolla is about 2.5 cm long, the back part is a honey bag, and the front part is widely spread. In oriental medicine, the tuber is used as a blood sedative, antispasmodic and analgesic, and is distributed through Korea and Northeast China to Siberia.

After washing with water, dehydrated and dried, powdered, and then sterilized by various known sterilization methods. As a preferred embodiment, the powder is 0.1 to 5 mm in diameter, and sterilization may be performed by high-pressure sterilization at 121 ° C. for 30 minutes. Corydalis and corydalis may be 50 to 150 parts by weight of corydalis based on 100 parts by weight of the golden age in dry powder form.

Phellinus Phellinus mycelium culture solution of the present invention can be prepared by species-cultivating Phellinus Phellinus spawn. The situation mushroom spawn can be maintained by subculture periodically until use. As a preferred embodiment, after inoculating the situation mushroom mycelium in a PDA (Potato Dextrose Agar) medium, passage at 3 to 4 week intervals at a temperature of 20 to 25 ° C. can be cultivated and maintained. The seed culture is a process of culturing the mycelium spawn of Phellinus Phellinus in storage prior to the main culture. As a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating spawn, that is, mycelium, into a medium for mycelium of Phellinus Phellinus, and then culturing it with rotational shaking.

The method of inoculating the Phellinus Phellinus mycelium of the present invention into sterilized golden rice and corydalis dry powder is aseptically inoculating a certain amount of the liquid culture obtained by the above-described Phellinus Phellinus culturing into sterilized golden rice and corydalis dry powder will be. For example, 30 to 70 parts by weight of a mycelium culture solution of Phellinus Phellinus may be inoculated with respect to 100 parts by weight of mixed dry powder of golden brown and corydalis in a flask container 5 to 10 times the volume.

After inoculating the Phellinus linteus mycelium, it undergoes a culture process. The culture conditions are preferably a temperature of 20 to 35 ° C, a relative humidity of 70 to 90%, and an incubation period of 7 to 45 days. As a preferred embodiment, 24 ° C. It can be cultured for 20 days in an incubator at a temperature of 85% relative humidity.

The fermented Phellinus and Corydalis mixture of Phellinus Phellinus mycelium, which has been fermented, has a shape in which the mycelium of Phellinus Phellinus is entirely covered on the surface of the mixed powder of Phellinus and Corydalis. An extract of the fermentation product can be prepared.

The solvent used for extraction may be water or lower ethanol, but hot water extraction is preferred. As a preferred embodiment, about 5 to 150 times the volume of hot water is added to the mixed fermented product of dried and pulverized Phellinus linteus mycelium and corydalis, shaken for 1 to 5 hours, and filtered or centrifuged to obtain an extract. . The above process may be performed 1 to 5 times, and preferably, the extraction may be repeated 3 times. The obtained extract is sterilized in the same manner as the sterilization process described above.

It is preferable that the functional cosmetic composition of the present invention further includes a mixed fermented product of golden rice and corydalis fermented by inoculation with mycelium of Cordyceps sinensis in addition to the above-described mixed fermented product of golden rice and corydalis fermented with mycelium of Phellinus Phellinus.

The mixed fermented product of the cordyceps sinensis mycelium inoculated with goldenrod and corydalis is: (1) drying and powdering the goldenrod and corydalis; (2) inoculating a culture solution of Cordyceps sinensis mycelium in a culture flask containing powdered geumnyeongja and corydalis; (3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) extracting with hot water and pulverizing.

The cordyceps is a fungus belonging to the ascomycete ergotomycete Cordyceps, specifically, Cordyceps spp., Paecilomyces spp., Isaria spp., Phytocordyceps genus ( Phytocordyceps spp.), etc. are classified into about 100 genera, and about 750 species are known worldwide.

In the present invention, all of these cordyceps can be used, and preferably, Cordyceps sinensis ( Cordyceps sinensis ), Cordyceps Milataris ( Cordyceps militaris ), Cordyceps scarabecola ( Cordyceps scarabaecola ), Cordyceps nutans ( Cordyceps nutans ), Cordyceps sphecocephala, Cordyceps tricentri , Paecilomyceps tenuipes , and Paecilomyces japonica . , More preferably, it is Paecilomyces japonica , also named snow cordyceps.

The Cordyceps sinensis mycelium culture solution of the present invention can be prepared by culturing the Cordyceps sinensis spawn. The Cordyceps sinensis spawn can be maintained by subculture periodically until use. As a preferred embodiment, the Cordyceps sinensis mycelium is inoculated into PDA (Potato Dextrose Agar) medium and then subcultured at 20 to 25 ° C. at intervals of 3 to 4 weeks can keep The seed culture is a process of culturing Cordyceps sinensis mycelium spawn prior to main culture. As a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating spawn, that is, mycelium, in a medium for Cordyceps sinensis mycelium, and then culturing it with rotational shaking.

The method of inoculating the mycelium of Cordyceps sinensis of the present invention into a mixed dried powder of sterilized goldenrod and corydalis is aseptically inoculating a certain amount of the liquid culture obtained by the above-described seed culture of Cordyceps sinensis into a sterilized mixed dry powder of goldenrod and corydalis is to do For example, 30 to 70 parts by weight of Cordyceps sinensis mycelium culture solution may be inoculated with respect to 100 parts by weight of mixed dried powder of gold and corydalis in a flask container of 5 to 10 times the volume.

After inoculating the mycelium of Cordyceps sinensis, it undergoes a culture process. The culture conditions are preferably a temperature of 20 to 35 ° C, a relative humidity of 70 to 90%, and a culture period of 7 to 45 days. As a preferred embodiment, a temperature of 24 ° C. It can be cultured for 20 days in an incubator with a relative humidity of 85%.

The mixed fermented product of the fermented cordyceps mycelium of golden brown and corydalis is a shape in which the surface of the mixed dry powder of the mixed dried powder of cordyceps mycelium is completely covered with the mycelium of cordyceps sinensis. An extract of the fermentation product can be prepared.

The solvent used for extraction may be water or lower ethanol, but hot water extraction is preferred. As a preferred embodiment, about 5 to 150 times the volume of hot water is added to the mixed fermented product of dried and pulverized cordyceps mycelium and corydalis, shaken for 1 to 5 hours, and filtered or centrifuged to obtain an extract. The above process may be performed 1 to 5 times, and preferably, the extraction may be repeated 3 times. The obtained extract is sterilized in the same manner as the sterilization process described above.

The functional cosmetic composition of the present invention is preferably selected from the group consisting of antioxidant, antibacterial, skin aging prevention and skin elasticity improvement.

The functional cosmetic composition is flexible lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, powder foundation, emulsion foundation, wax foundation, cleansing cream, cleansing foam, cleansing water, pack, spray, powder, pact, lip gloss It is preferably a formulation selected from the group consisting of rose, lipstick, shadow, shampoo and rinse.

As a preferred embodiment, the functional cosmetic composition of the present invention may be prepared to include 0.001 to 50% by weight of a mixed fermented product of Geumnyeongja and Corydalis by inoculation of Phellinus Phellinus mycelium.

As another preferred embodiment, the functional cosmetic composition of the present invention is prepared to contain a mixture of a mixed fermented product of Geumnyeongja and Corydalis by inoculation of Phellinus Phellinus mycelium and a mixed fermented product of Geumnyeongja and Corydalis by inoculation with Mycelium of Cordyceps sinensis at 0.001 to 50% by weight It may be, and the mixing ratio of the mixture may be in the range of 1:99 to 99:1.

Appropriate formulation stability can be secured within this content range, and desired functionality can be expected.

The functional cosmetic composition of the present invention, in addition to the mixed fermented product of golden brown and corydalis by inoculation of mycelium of Phellinus linteus of the present invention and the mixed fermented product of golden brown and corydalis by inoculation with mycelium of Cordyceps sinensis, antioxidants within the range that does not impair the effective activity of the present invention , For the effect of preventing skin aging and improving skin elasticity, known natural extracts or other ingredients may be additionally included and used.

In addition, the composition may include ingredients commonly added to cosmetic compositions, for example, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, Cosmetics such as fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives; It can be molded into a specific dosage form including adjuvants or carriers commonly used in the field of dermatology.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, pack, soap, surfactant- It may be formulated as containing cleansing, oil and spray, etc., but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydrocarbon, propellants such as propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

Hereinafter, the present invention will be described in more detail through preferred embodiments. The following examples describe a preferred embodiment of the present invention, and the scope of the present invention is not limited and interpreted by the following examples.

[Example]

1. Mixed feet of Geumnyeongja and Corydalis by Sanghwang mushroom mycelium filial hydrothermal extract manufacturing

Longevity Sanghwang Mushroom ( Phellinus baumii ) was used by receiving a strain under passage from Pharmbios Co., Ltd. It was cultured using PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25 ° C., subcultured at 4-week intervals, and stored at 4 ° C. In a 500ml flask, after inoculating the mycelium of the longevity Phellinus Phellinus in a 100ml volume of mushroom mycelium solid medium (1 cm × 1 cm, 3 pieces), in a rotary shaking incubator (Daihan Labtech Co.Korea, 25±1℃, 130 rpm) It was cultured for 7 days and used as a liquid spawn.

Geumnyeongja and Corydalis were purchased from the Daegu herbal medicine market, washed with water to remove impurities before use, dehydrated, and then powdered. 50 g of golden rice powder and 50 g of corydalis powder were put in a 3L culture vessel, autoclaved at 121 ° C. for 30 minutes, cooled at room temperature, and 60 ml of the prepared liquid spawn was aseptically inoculated into 100 g of the sterilized mixed powder. Fermentation was carried out for 20 days in an incubator at 24 ° C and 85% relative humidity. After fermentation, 10 times the volume of hot water was added, shaken for 2 hours, immersed for 72 hours, centrifuged at 10,000 rpm for 10 minutes, and the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure to prepare a powder. used

2. Preparation of Hot Water Extract of Mixed Fermentation of Geumnyeongja and Corydalis by Cordyceps Sinensis Mycelium

Snow cordyceps ( Paecilomyces japonica ) was used by receiving strains under passage from Pharmbios Co., Ltd. It was cultured using PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25 ° C., subcultured at 4-week intervals, and stored at 4 ° C. In a 500ml flask, after inoculating the mycelium of 100ml of mushroom mycelium solid medium (1 cm × 1 cm, 3 pieces), the mycelium of Passage Cordyceps sinensis was inoculated, and then 7 It was cultured for a day and used as a liquid spawn.

Geumnyeongja and Corydalis were purchased from the Daegu herbal medicine market, washed with water to remove impurities before use, dehydrated, and then powdered. 50 g of golden rice powder and 50 g of corydalis powder were put in a 3L culture vessel, autoclaved at 121 ° C. for 30 minutes, cooled at room temperature, and 60 ml of the prepared liquid spawn was aseptically inoculated into 100 g of the sterilized mixed powder. Fermentation was carried out for 20 days in an incubator at 24 ° C and 85% relative humidity. After fermentation, 10 times the volume of hot water was added, shaken for 2 hours, immersed for 72 hours, centrifuged at 10,000 rpm for 10 minutes, and the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure to prepare a powder. used

3. Manufacture of extracts of gold rhizome and corydalis in hot water

Geumnyeongja and Corydalis were purchased from the Daegu herbal medicine market, washed with water to remove impurities before use, dehydrated, and then powdered. After adding 10 times the volume of hot water to each of the golden bell pepper powder and corydalis powder, shaking for 2 hours, soaking for 72 hours, and centrifuging at 10,000 rpm for 10 minutes, the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure. Therefore, it was used after preparing a powder of hot-water extract of Geumnyeongja and a powder of hot-water extract of Corydalis.

4. Preparation of samples

Samples according to the components shown in Table 1 below were prepared and used for the activity test. The control group means the sample untreated group.

[Table 1] Components and contents of samples

5. Antioxidant activity

Each sample was evaluated by measuring DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging ability and FRAP activity. In the case of DPPH scavenging ability, 380 μl of a 2x10 ^-4 M DPPH solution dissolved in 99.5% ethanol was added to 20 μl of the sample, mixed, and reacted at 37 ° C for 30 minutes, followed by a microplate reader (Asys Hitech, Expert96, Asys Co., Austria) at 516 nm. ) was used to measure absorbance. FRAP activity was performed on the prepared samples according to a known method. DPPH free radical scavenging ability and FRAP activity were expressed as percentages as shown in Table 2 below.

[Table 2] DPPH radical scavenging activity and FRAP activity

6. Evaluation of dandruff inhibitory activity

Since dandruff appears when Pityrosporum yeast, which is often present on the scalp, overgrows due to causes such as stress, its inhibitory activity was evaluated. A Pityrosporum ovale strain purchased from ATCC (ATCC12078) and preserved was used. The seed culture of the strain was used by culturing in Luria-Bertani broth for 7 days. Luria-Bertani agar was autoclaved, solidified in a petri dish, and seed cultured strains were plated. An 8 mm paper disc was placed in the center of the Petri dish, and 20 μl of the sample was added and cultured at 35 ° C for 3 days. The degree of inhibition was evaluated by measuring the clear zone according to the inhibition of the Pityrosporum strain of the sample with a caliper, and the results are shown in Table 3.

[Table 3] Evaluation of antibacterial activity

7. Prevents skin aging and improves skin elasticity

In order to evaluate the effect of preventing skin aging and improving skin elasticity, the results of evaluating the elastase inhibitory activity (%) of the prepared samples are shown in Table 4 below.

[Table 4] Elastase inhibitory assay

Claims (6)

A functional cosmetic composition comprising a mixed fermented product of Geumnyeongja (金鈴子) and Corydalis (玄胡索) by inoculation of Phellinus Phellinus mycelium. The functional cosmetic composition according to claim 1, wherein the mixed fermented product of Geumnyeongja and Corydalis by inoculation of the Phellinus Phellinus mycelium is obtained by including the following steps:
(1) drying and pulverizing Geumnyeongja and Corydalis;
(2) inoculating the mycelium culture solution of Phellinus Phellinus in a culture flask containing powdered Geumnyeongja and Corydalis;
(3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and
(4) Powdering by hot water extraction. The functional cosmetic composition according to claim 1, further comprising a mixed fermented product of golden bellflower and corydalis by inoculation of Cordyceps sinensis mycelium. [Claim 4] The functional cosmetic composition according to claim 3, characterized in that the mixed fermented product of golden bellflower and corydalis by inoculation of the mycelium of Cordyceps sinensis is obtained by including the following steps:
(1) drying and pulverizing Geumnyeongja and Corydalis;
(2) inoculating a culture solution of Cordyceps sinensis mycelium in a culture flask containing powdered geumnyeongja and corydalis;
(3) culturing under conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and
(4) Powdering by hot water extraction. The functional cosmetic composition according to any one of claims 1 to 4, wherein the functionality is selected from the group consisting of anti-oxidation, anti-bacterial, anti-aging, and improvement of skin elasticity. The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is softening lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, powder foundation, emulsion foundation, wax foundation, cleansing cream, cleansing foam , Functional cosmetic composition characterized in that the formulation is selected from the group consisting of cleansing water, pack, spray, powder, pact, lip gloss, lipstick, shadow, shampoo and rinse.