KR20230010471A - Cosmetic composition comprising champsocephalus gunnari extract or notothenia rossii extract as an active ingredient for improving wrinkles - Google Patents

Abstract

The present invention relates to a cosmetic composition for reducing skin wrinkles, containing, as an active ingredient, a Champsocephalus gunnari extract or a Notothenia rossii extract. The Champsocephalus gunnari extract or Notothenia rossii extract of the present invention exhibits a collagen biosynthesis enhancement effect and MMP-1 suppression activity at a very low concentration and has an effect of protecting cells from UV rays, and thus a composition containing the same as an active ingredient can be usefully used as a functional cosmetic composition for reducing wrinkles. Especially, the extract of the present invention is a natural material derived from Antarctic fish, and the cosmetic composition of the present invention, containing the same, has a safety advantage even in a long-term use.

Description

Cosmetic composition comprising champsocephalus gunnari extract or notothenia rossii extract as an active ingredient for improving wrinkles}

The present invention relates to a cosmetic composition for improving skin wrinkles comprising an extract of Antarctic Meckel's smelt ( Champsocephalus gunnari ) or an extract of Antarctic marbled female fish ( Notothenia rossii ) as an active ingredient.

The skin has the function of protecting against temperature, humidity, antigens and ultraviolet rays while being in direct contact with the external environment. However, due to physical and chemical stimuli such as external pollutants, ultraviolet rays, and stress, skin function is deteriorated, and cell proliferation and immune activity are weakened with age, so damage to skin cells cannot be quickly restored and the overall deterioration of skin components This causes wrinkles, loss of elasticity, and keratinization of the skin.

Skin aging is largely divided into intrinsic aging and extrinsic aging. Intrinsic aging is a naturally occurring aging phenomenon, clinically characterized by a decrease in elasticity, rough skin texture, deep wrinkles, and pigmentation. Extrinsic aging is an aging phenomenon caused by external environmental factors such as ultraviolet rays, reactive oxygen species, and stress. In recent years, the amount of UV has increased due to rapid industrialization, air pollution, and destruction of the ozone layer. By generating strong oxidizing substances such as oxygen species, it induces destruction of connective tissue formation such as skin collagen, inhibition of cell membrane function, acceleration of DNA mutation, modification of protein action, and modification of intracellular signaling substances, resulting in wrinkle formation and pigmentation in humans. etc., causing skin aging. In order to prevent such skin damage, cosmetics or skin protection agents containing various skin protection ingredients such as retinol, retinoids, vitamin C, flavonoids, tocopherol, and coenzyme Q10 have been developed.

Particularly, ultraviolet rays (UV) of sunlight are one of the well-known causes of aging, and in the skin exposed to ultraviolet rays for a long time, the stratum corneum thickens, and collagen and elastin, which are the main components of the skin, are denatured and lose elasticity of the skin. Collagen and elastin are regulated by various factors. Expression of matrix metallo proteases such as collagenase and elastase degrade collagen and elastin, resulting in A decrease in collagen content occurs. Various substances have been developed and used for the purpose of suppressing the reduction of collagen and elastin, which are the cause of such a decrease in elasticity. It is also applied to show the effect of increasing elasticity. However, these retinoids have the disadvantage of being sensitive to skin irritation and allergies even when only a small amount is applied to the skin.

Recently, after exploring physiologically active substances such as whitening, anti-aging, anti-wrinkle, anti-oxidation, and anti-inflammation through extraction process and fermentation technology using natural substances to develop cosmetics that are relatively less toxic and nature-friendly, they are used as cosmetic materials. are using

On the other hand, Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ) are fish that live only in Antarctica as marine living resources.

Accordingly, the inventors of the present invention, while searching for a natural substance with excellent skin wrinkle improvement effect without side effects in the body, paid attention to Antarctic fish and experimented on its pharmacological effect. Notothenia rossii ) has excellent collagen biosynthesis enhancement effect and MMP-1 inhibitory activity at very low concentrations, and also has the effect of protecting cells from ultraviolet (UVB), so it has functionality for improving skin wrinkles The present invention was completed by confirming that there is sufficient value as a cosmetic material.

Korean Patent Publication No. 10-2020-0104467 Korean Registered Patent No. 10-2018-0008557

Accordingly, an object of the present invention is to provide a cosmetic composition capable of effectively improving skin wrinkles.

In order to achieve the object of the present invention as described above,

The present invention provides a cosmetic composition for improving skin wrinkles comprising an extract of Antarctic Meckel's smelt (Champsocephalus gunnari ) or an extract of Antarctic marble-patterned female fish ( Notothenia rossii ) as an active ingredient.

In one embodiment of the present invention, the extract may be extracted with one or more solvents selected from the group consisting of lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, water and hexane.

In one embodiment of the present invention, the extract may have a collagen biosynthesis enhancing effect and MMP-1 inhibitory activity.

In one embodiment of the present invention, the extract may have an effect of protecting cells from ultraviolet rays.

In one embodiment of the present invention, the cosmetic composition is flexible lotion, gel, water-soluble liquid, milk lotion, nourishing cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, face-type anhydrous product, solid anhydrous product , Oil dispersion in aqueous phase using spherules, ionic lipid vesicles, nonionic lipid vesicles, ointments, cleansing foams, cleansing water, packs, body oils, oil-in-water makeup bases, water-in-oil makeup bases, foundations, toners It may be one type of formulation selected from the group consisting of cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

The Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or the Antarctic marbled female fish ( Notothenia rossii ) extract of the present invention is excellent in collagen biosynthesis increasing effect and MMP-1 inhibitory activity even at very low concentrations, and protects cells from ultraviolet (UV) In the case of a composition containing this as an active ingredient, it can be usefully used as a functional cosmetic composition for wrinkle improvement. In particular, the extract of the present invention is a natural material derived from Antarctic fish, and the cosmetic composition of the present invention containing it has the advantage of being safe for long-term use.

1 is a graph showing the MMP-1 inhibitory activity of the Antarctic fish extract (25 μg/ml) of the present invention (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
2 is a graph showing the effect of increasing collagen biosynthesis of the Antarctic fish extract (25 μg/ml) of the present invention (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6 : NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
3 is a result of measuring cell viability according to treatment with the Antarctic fish extract (25 μg/ml) of the present invention after UVB (125 mJ/cm ² ) irradiation (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
4 is a result of measuring cell viability according to treatment with the Antarctic fish extract (25 μg/ml) of the present invention after UVB (144 mJ/cm ² ) irradiation (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).

The present invention is characterized in that it provides a cosmetic composition for improving skin wrinkles containing an extract of Antarctic Meckel smelt ( Champsocephalus gunnari ) or an extract of Antarctic marble-patterned female fish ( Notothenia rossii ) as an active ingredient.

The "Antarctic Meckel smelt extract or Antarctic marbled smelt extract" of the present invention is directly extracted and separated from Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled smelt ( Notothenia rossii ) using an extraction and separation method known in the art. The 'extract' defined in the present invention can be obtained from various parts such as skin, brain, liver, stomach, etc. of the Antarctic fish using an appropriate solvent. extracts, and includes, for example, crude extracts of various parts (skin, brain, liver, stomach), polar solvent-soluble extracts, or non-polar solvent-soluble extracts of the Antarctic fish.

Any pharmaceutically acceptable organic solvent may be used as a suitable solvent for extracting the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention, and water or an organic solvent may be used, but is not limited thereto. , For example, alcohol having 1 to 4 carbon atoms including purified water (water), methanol, ethanol, propanol, isopropanol, butanol, etc., acetone, Various solvents such as ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane can be used alone or in combination. can Preferably, it is good to use hexane.

As an extraction method, any one of methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the preparation method of the extract of the present invention, and any known method may be used.

For example, the Antarctic Meckel smelt extract or Antarctic marbled female fish extract of the present invention is obtained by converting the primary extract extracted by the hot water extraction or solvent extraction method into a powder state by an additional process such as vacuum distillation and freeze drying or spray drying. can be manufactured In addition, a fraction further purified from the primary extract using various chromatography methods such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography you can also get

Therefore, in the present invention, the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract is a concept that includes all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and dilutions, concentrates, or dried products thereof.

In one embodiment of the present invention, the extract is obtained by freezing and drying the skin, brain, liver and stomach tissues of Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ), and then pulverizing and then adding hexane to extract. there is.

The present inventors found that the Antarctic Meckel smelt extract or the Antarctic marble-patterned mackerel extract had collagen biosynthesis increasing effect and MMP-1 inhibitory activity (see Experimental Example 1), and cell protection effect according to UVB irradiation (see Experimental Example 2). proved through

The composition of the present invention may include ingredients commonly used in cosmetic compositions, as well as Antarctic Meckel smelt extract or Antarctic marbled smelt extract, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. A phosphorus adjuvant, and a carrier may be included.

In addition, the composition of the present invention, in addition to the above-mentioned active ingredients (Antarctic Meckel smelt extract or Antarctic marble-patterned female fish extract), may be used by mixing a skin protectant that has been used conventionally to the extent that it does not damage the skin protection effect by reacting with the active ingredient. there is.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, foundations, etc., as well as cosmetics such as cleansing, face wash, soap, treatment, and beauty essence. etc.

Specific formulations of the cosmetic composition of the present invention include flexible lotion, gel, water-soluble liquid, milk lotion, nourishing cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, solid anhydrous product, spherules Oil dispersions in the aqueous phase used, ionic lipid vesicles, nonionic lipid vesicles, ointments, cleansing foams, cleansing water, packs, body oils, oil-in-water makeup bases, water-in-oil makeup bases, foundations, skin covers, lipsticks, It includes one formulation selected from the group consisting of lip gloss, face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

According to a preferred embodiment of the present invention, the content of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled female fish extract) of the present invention is 0.0001-20% by weight, preferably 0.5-20%, based on the total weight of the composition, More preferably, it is 1.0-20 weight%. If the content of the active ingredient (Antarctic Meckel smelt extract or Antarctic marble-patterned female fish extract) is less than 0.0001% by weight, the wrinkle improvement effect is greatly reduced, and if it exceeds 20% by weight, it may cause skin irritation, and problems in formulation this can happen

On the other hand, the cosmetic composition according to the present invention may be formulated by stabilizing the active ingredient (Antarctic Meckel smelt extract or Antarctic marble-patterned mackerel extract) inside the nanoliposome. When the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled smelt extract) is contained inside the nanoliposome, the components of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled smelt extract) are stabilized, resulting in formation of sediment, discoloration, Problems such as off-odor can be solved, and the solubility and transdermal absorption rate of components can be increased, so that the efficacy expected from the extract can be maximized.

In the present invention, the nanoliposome has the form of a conventional liposome, and means a liposome having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposome is 50 to 300 nm. When the average particle diameter of the nanoliposome exceeds 300 nm, improvement of skin penetration and improvement of formulation stability among the technical effects to be achieved in the present invention are very weak.

According to the present invention, the nanoliposome used to stabilize the extract may be prepared by a mixture containing a polyol, an oily component, a surfactant, a phospholipid, a fatty acid, and water.

Polyols used in the nanoliposomes of the present invention are not particularly limited, and are preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isopropylene glycol, pentylene glycol, erythritol, and xylitol. , sorbitol, and at least one selected from the group consisting of mixtures thereof. The amount used is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of the nanoliposome.

Various oils known in the art may be used as the oil component used in the preparation of the nanoliposomes of the present invention, preferably hydrocarbon-based oils such as hexadecane and paraffin oil, ester-based synthetic oils, Silicone oils such as dimethicone and cyclomethicone, animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils , phytosphingosine, sphingonoid lipids such as sphingosine and sphinganine, cerebroside cholesterol, sitosterol cholesteryl sulfate, sitosteryl sulfate, C10-40 fatty alcohols and mixtures thereof. The amount used may be 1.0 to 30.0% by weight, preferably 3.0 to 20.0% by weight, based on the total weight of the nanoliposome.

Any surfactant known in the art may be used as the surfactant used in the preparation of the nanoliposome of the present invention. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkylacylglutamates, alkylphosphates, alkylactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount used may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.

Phospholipids, which are another component used in the preparation of the nanoliposomes of the present invention, are amphiphilic lipids, natural phospholipids (e.g., egg yolk lecithin or soybean lecithin, sphingomyelin) and synthetic phospholipids (e.g., dipalmitoylphosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated or saturated lecithin of natural origin extracted from soybean or egg yolk is preferred. Typically, naturally-derived lecithin has an amount of phosphatidylcholine of 23 to 95% and an amount of phosphatidylethanolamine of 20% or less. In the preparation of the nanoliposome of the present invention, the amount of phospholipid used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight, based on the total weight of the nanoliposome.

The fatty acids used in the preparation of nanoliposomes of the present invention are higher fatty acids, preferably C12-22 alkyl chain saturated or unsaturated fatty acids, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. include The amount used may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of the nanoliposome.

Water used in the preparation of the nanoliposome of the present invention is generally deionized distilled water, and the amount used may be 5.0 to 40% by weight based on the total weight of the nanoliposome.

Nanoliposomes may be prepared through various methods known in the art, but most preferably, they are prepared by applying a mixture containing the above components to a high-pressure homogenizer. The preparation of nanoliposomes by a high-pressure homogenizer can be carried out under various conditions (e.g., pressure, number of times, etc.) depending on the desired particle size, preferably 1 to 5 times under a pressure of 600 to 1200 bar. Nanoliposomes can be prepared by passing through.

The cosmetic composition for improving skin wrinkles according to the present invention may contain 0.1 to 20.0% by weight of the active ingredient (Antarctic Merkel smelt extract or Antarctic marble-patterned female fish extract) based on the total weight of nanoliposomes.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited to these examples.

<Example 1>

Preparation of Antarctic fish extract of the present invention

Frozen Antarctic fish Notothenia rossii (hereinafter simply abbreviated as 'NR') and Champsocephalus gunnari (hereinafter simply abbreviated as 'CG') were each thawed. In the case of NR, it was directly collected from the coast of King Sejong Station in Antarctica, and in the case of CG, by-caught fish was delivered from a deep-sea fishing company that fishes krill. Each individual was dissected to obtain tissue samples (skin, brain, liver, stomach), washed with distilled water, and freeze-dried. From the dried tissue samples, NR: skin 13.20 g, brain 0.35 g, liver 2.74 g, stomach 3.12 g, CG: skin 8.50 g, brain 0.22 g, liver 8.36 g, stomach 2.78 g, respectively. After each sample was crushed using a mortar and pestle, each was transferred to a 200 mL glass bottle, and then 200 mL of 95% hexane was added and extracted twice a day at room temperature (24 hr X 2). Each of the extracts obtained through the above process was transferred to a round flask and the solvent was completely removed using a vacuum concentrator. As a concentrated extract from which the solvent was removed, NR: skin 0.18 g, brain 0.13 g, liver 0.24 g, stomach 0.21 g, CG: skin 0.41 g, brain 0.08 g, liver 1.78 g, stomach 0.52 g were finally obtained, which Experiments were conducted using

Antarctic fish extract of the present invention extract sample extraction solvent Pisces group One CG skin hexane 2 brain hexane 3 liver hexane 4 stomach hexane 5 NR skin hexane 6 brain hexane 7 liver hexane 8 stomach hexane

<Experimental Example 1>

Anti-wrinkle effect of the Antarctic fish extract of the present invention

In this experiment, the skin wrinkle improvement effect of the Antarctic fish extract of the present invention prepared through <Example 1> was examined.

<1-1> Measurement of MMP-1 inhibitory activity

The skin wrinkle improvement effect was evaluated by the inhibitory effect on matrix metalloproteinase-1 (hereinafter simply abbreviated as 'MMP-1'), one of the collagenase enzymes that decompose and destroy collagen.

Specifically, a total of 8 types of Antarctic fish prepared through <Example 1> after treating Normal Human Dermal Fibroblasts (hereinafter simply abbreviated as 'NHDF') with UVB of 125 mJ/cm ² . The extract was treated at a concentration of 25μg/ml and cultured for a total of 72 hours, then the cell medium was collected from each well, and the medium conditioned using a commercially available ELISA kit (Human Total MMP-1 kit) was used to obtain MMP-1 The concentration of was analyzed three times for each sample.

As a result, as shown in FIG. 1 and Table 2, MMP-1 was remarkably increased when treated with UVB, and MMP-1 increased by UVB treatment was significantly increased according to the treatment of the 8 types of Antarctic fish extracts of the present invention. confirmed to decrease.

Concentration of MMP-1 according to treatment of Antarctic fish extract of the present invention (pg/ml) NCtr
(UVB-) Ctr
(UVB+) CG skin CG brain CG liver Above CG NR skin NR brain NR liver Above NR 3474.21 4746.48 2925.85 3261.02 3295.71 3216.54 2995.63 3091.67 2727.64 3310.56

<1-2> Measurement of degree of collagen biosynthesis

Collagen is synthesized into procollagen within the cell, then secreted out of the cell and polymerized into collagen fibers. Such collagen is a structural protein constituting the skin, and wrinkles of the skin are improved when the content of collagen in the skin is high and the activity of collagenase is low. In this experiment, in order to examine the skin wrinkle improvement effect, the degree of collagen biosynthesis was confirmed by measuring the Procollagen type-IC peptide (hereinafter simply abbreviated as 'PIP').

In detail, NHDF was treated with UVB of 144 mJ/cm ² and then treated with a total of 8 types of Antarctic fish extracts prepared in <Example 1> at a concentration of 25 μg/ml and cultured for a total of 72 hours, then each well The cell medium was collected from, and the amount of PIP was analyzed three times for each sample using a medium conditioned using a commercially available ELISA kit (R&D Systems Inc.; Procollagen).

As a result, as shown in FIG. 2 and Table 3, it was confirmed that the amount of PIP was remarkably reduced when UVB was treated, and when the Antarctic fish extract of the present invention was treated after UV irradiation, extract 5 (NR skin) and extract 7 (NR liver) and extract 8 (NR top) showed a significantly increased amount of PIP in the experimental group.

Amount of PIP (ng/ml) according to Antarctic fish extract treatment of the present invention NCtr
(UVB-) Ctr
(UVB+) CG skin CG brain CG liver Above CG NR skin NR brain NR liver Above NR 454.33 297.67 240.83 255.50 193.83 246.25 485.58 271.33 533.10 505.83

<Experimental Example 2>

Cytoprotective effect of the Antarctic fish extract of the present invention

In this experiment, in order to examine the cell protection effect according to UVB irradiation of a total of 8 types of Antarctic fish extracts prepared in <Example 1>, cell viability was investigated through MTT assay.

NHDF (Normal Human Dermal Fibroblasts) cells, a human skin fibroblast cell line, were treated with UVB 125 mJ/cm ² or After treatment with 144 mJ/cm ² , a total of 8 types of Antarctic fish extracts prepared in <Example 1> were treated at a concentration of 25 μg/ml and cultured for a total of 72 hours. Then, the medium was removed, and 200 μL of 1 mg/mL MTT solution was added to each well, followed by reaction at 37° C. for 2 hours in the presence of 5% CO ₂ . Thereafter, the substrate-containing medium was removed and 200 μL of DMSO was added to each well to dissolve the formazan crystals. The plate was shaken on an orbital shaker for 10 minutes at room temperature. The absorbance of 100 μL aliquots of formazan dissolved in DMSO was quantified by measuring the optical density (OD) at 570 nm using an ELISA reader (Molecular Devices E09090, San Francisco, CA, USA).

As a result, as shown in FIG. 3, it was confirmed that the cell viability (%) was reduced by about 25% by UVB (125 mJ/cm ² ) irradiation, and when the Antarctic fish extract of the present invention was treated after UV irradiation, the extract Excluding 1 (CG Skin) and extract 6 (NR Brain), it was confirmed that the cell viability (%) increased compared to UVB irradiation alone. In particular, the experimental group treated with extract 2 (CG brain), extract 4 (CG stomach), and extract 8 (NR stomach) showed significantly increased cell viability (%), and through this, among the Antarctic fish extracts of the present invention, In the case of extract 2 (CG brain), extract 4 (CG top), and extract 8 (NR top), it was judged to have an effect of protecting cells from ultraviolet rays at a concentration of 25 μg / ml.

In addition, as shown in FIG. 4, it was confirmed that the cell viability (%) was reduced by about 25% by UVB (144 mJ/cm ² ) irradiation, and when the Antarctic fish extract of the present invention was treated after UV irradiation, all It was confirmed that the cell viability (%) increased in the experimental group compared to UVB irradiation alone.

Through the above results, in the case of the Antarctic fish extract of the present invention, it was confirmed that there was a cytoprotective effect according to UVB irradiation and no cytotoxicity.

<Experimental Example 3>

Fatty Acid Analysis of Antarctic Fish Extracts of the Present Invention

For fatty acid analysis, lipids were extracted according to the method of Folch et al. (J. Folch, M. Lees, G. H. S. Stanley. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 497-509 .).

Briefly, methyl esterification was performed with Boron trifluoride-methanol solution (BF3- MeOH, 14%) and analyzed by gas chromatography (Varian (3400) / Shimadzu (GC-17A)), and the identification of fatty acids was performed under the same conditions. The peak area ratio was calculated by comparing the retention time of the standard product. Analysis was performed using Omegawax 250 (0.25 mm × 30 m), column temperature 180 ℃, inlet temperature 250 ℃, detector temperature 250 ℃, heating conditions 180 ℃ (8min) → 3 ℃ / min → 225 ℃ (15min), A condition of split 100:1 was used.

The results are shown in detail in Table 4 below.

Fatty Acid Names and Symbols Number of carbons: number of double bonds local mountain name English name (official name) Young name (family name) 12:00 lauric acid Lauric acid Dodecanoic acid 14:00 myristic acid Myristic acid Tetradecanoic acid 16:00 palmitic acid Palmitic acid Hexadecanoic acid 18:00 stearic acid Stearic acid Octadecanoic acid 20:00 arachidic acid Arachidic acid Eicosanoic acid 14:01 myristoleic acid Myristoleic acid Tetradecenoic acid 16:01 palmitoleic acid Palmitoleic acid Hexadecenoic acid 18:01 oleic acid Oleic acid Octadecenoic acid 20:01 eicosenoic acid Eicosenoic acid Eicosenoic acid 22:01 Erucic acid (docosenic acid) Erucic acid Docosenoic acid 18:2n-6 linoleic acid Linoleic acid 9,12-Octadecadienoic acid 18:3n-3 alpha linolenic acid α-Linolenic acid 9,12,15-Octadecatrienoic acid 18:4n-3 stearidonic acid Stearidonic acid 6,9,12,15-Octadecatetraenoic acid 20:4n-6 arachidonic acid Arachidonic acid 5,8,11,14-Eicosatetraenoic acid 20:5n-3 eicosapentaenoic acid Eicosapentaenoic acid 5,8,11,14,17-Eicosapentaenoic acid 22:5n-3 Docosapentaenoic acid Docosapentaenoic acid 7,10,13,16,19-Docosapentaenoic acid 22:6n-3 Docosahexaenoic acid Docosahexaenoic acid 4,7,10,13,16,19-Docosahexaenoic acid

Formulation Example 1: Soft lotion (skin lotion)

As shown in the table below, softening lotion was prepared according to a conventional method.

Formulation example of softening lotion of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 1,3-butylene glycol 6.0 glycerin 4.0 DL-panthenol 0.3 allantoin 0.1 polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 fragrance, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrition lotion (milk lotion)

As shown in the table below, nutrient lotion was prepared according to a conventional method.

Nutritional longevity formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitastearate 0.5 hydrogenated vegetable oil 4.0 mineral oil 5.0 trioctanoin 2.0 dimethicone 0.8 Tocopherol Acetate 0.5 carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 sodium hyaluronate 5.0 triethanolamine 0.12 Uniside-U 13 0.02 incense a very small amount Distilled water to 100

Formulation Example 3: Nutrition Cream

As shown in the table below, a nutritious cream was prepared according to a conventional method.

Nutritional cream formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 Pro-type glycerin monostearate 1.5 Cetearyl Alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitastearate 0.6 isostearyl isosterate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 Uniside-U13 0.02 incense a very small amount Distilled water to 100

Formulation Example 4: Massage Cream

As shown in the table below, a massage cream was prepared according to a conventional method.

Massage cream formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 propylene glycol 6 glycerin 4.0 triethanolamine 0.5 beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan Sesquioleate 2.5 Cetearyl Alcohol 2.0 liquid paraffin 30.0 carboxyvinyl polymer 0.5 incense a very small amount Distilled water to 100

Formulation Example 5: Pack

As shown in the table below, packs were prepared according to conventional methods.

Pack formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 glycerin 10.0 betaine 5.0 REG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 80. carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 Uniside-U13 0.01 incense a very small amount Distilled water to 100

So far, the present invention has been looked at with respect to its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (5)

A cosmetic composition for improving skin wrinkles comprising an extract of Antarctic Meckel smelt ( Champsocephalus gunnari ) or an extract of Antarctic marbled female fish ( Notothenia rossii ) as an active ingredient. According to claim 1,
The extract is a cosmetic composition for improving skin wrinkles, characterized in that extracted with one or more solvents selected from the group consisting of lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, water and hexane. According to claim 1,
The extract is a cosmetic composition for improving skin wrinkles, characterized in that it has a collagen biosynthesis enhancing effect and MMP-1 inhibitory activity. According to claim 1,
The extract is a cosmetic composition for improving skin wrinkles, characterized in that it has an effect of protecting cells from ultraviolet rays. According to any one of claims 1 to 4,
The cosmetic composition is a flexible lotion, gel, water-soluble liquid, milk lotion, nourishing cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, solid anhydrous product, oil in aqueous phase using spherules Dispersion, ionic lipid vesicles, nonionic lipid vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water makeup base, water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder , A cosmetic composition for improving skin wrinkles, characterized in that it is one type of formulation selected from the group consisting of two-way cake, eye shadow, mascara, cheek color and eyebrow pencils.

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