JP5710197B2 - Fish extract, rough skin improving agent, ceramide production promoter, skin external preparation containing these, cosmetics - Google Patents

Description

The present invention relates to a novel fish extract to be applied to the skin, and a rough skin improving agent, a ceramide production promoter, a skin external preparation and a cosmetic using the same.

Fish is used in various ways as an important food resource in Japan. For example, an extract obtained by extracting bonito and dried fish with water is widely used as a soup stock in Japanese cuisine. In recent years, when bonito is ingested orally, there is a possibility of recovery from fatigue, the possibility of improving dry skin and rough skin, and the functionality when ingested as a food has been shown (Non-patent Document 1). In addition, a nourishing tonic food and a hair-restoring food characterized by containing a raw material obtained by drying anchovy dried and then powdered are also known (Patent Document 1).

On the other hand, fish have been known for a long time to use scales as glitter powder and to blend oil into cosmetics. However, these have a fish-specific odor, and technology for removing them has been developed (Patent Document 2), but they have not been completely solved yet, and when extracted with an organic solvent, a water-soluble moisturizing component is included. There was a problem that I could not. Recently, it has become possible to extract collagen / proteoglycan from fish skin, cartilage, etc. (Patent Documents 3 and 4) Applying an extract extracted with an aqueous solvent to the skin after drying the fish, The effect of this extract on the skin is not yet known.

Ceramide is a sphingolipid that exists between cells in the stratum corneum, and it is known that the stratum corneum can exhibit a moisturizing function and a barrier function by forming a lamella structure between the corneocytes. Decrease in the production amount of ceramide leads to skin dryness, which harms skin health as well as cosmetics. Attempts have also been made to supply reduced ceramide from the outside, but there are problems such as long-term effects not being observed and low stability. Conventionally, various ceramide production promoters have been studied, and blending into an external preparation for skin has been proposed. (Patent Documents 5 and 6) However, the effect was not satisfactory.

JP 2008-1000097 A JP 2009-91316 A JP 2006-152108 A JP 2003-299497 A JP 2002-370998 A JP 2000-169359 A

Health & Nutrition Food Research (VOL.9 NO.1 2006)

It is an object of the present invention to provide a novel fish extract to be applied to the skin as an effective utilization of natural resources, and a rough skin improving agent, a ceramide production promoter, a skin external preparation and a cosmetic using the same. .

As a result of intensive studies by the present inventors to solve the above-mentioned problems, it has been found that an aqueous solvent extract of dried fish has a high ceramide production promoting action, a moisturizing and lustrous effect on the skin, and an action to improve rough skin. The present invention has been completed.
That is, in order to solve the above-mentioned problems, the present invention provides an extract for application to the skin, which is characterized in that dried fish is extracted with an aqueous solvent.
Moreover, this invention provides the ceramide production promoter and skin roughening agent which use the said extract as an active ingredient, and provides the skin external preparation and cosmetics containing the said extract.

ADVANTAGE OF THE INVENTION According to this invention, when applied to skin, it becomes possible to give a high ceramide production promoting effect, moisturize and gloss the skin, improve rough skin, and provide an external preparation for skin and cosmetics containing these. be able to.

Hereinafter, the present invention will be described in detail. In the present specification, a numerical range represented by using “to” means a range including numerical values described before and after “to” as a lower limit value and an upper limit value.

The present invention relates to an extract obtained by extracting dried fish with an aqueous solvent. The purpose of drying is to remove water from the fish, and the method is not particularly limited. For example, it can be exposed to the sun or dried using natural or artificial cold or warm air. Before drying the fish, it may be compressed, boiled, steamed, etc., or dried using smoke generated when wood is heated. Further, the water may be removed by using a so-called freeze-drying method in which water is removed by placing the fish under reduced pressure, or by wrapping in a resin film having a specific hole diameter for removing only water. Although the site | part used when extracting is not specifically limited, The fish meat part of a fish trunk part is preferable. The fish may be cut to an appropriate size for efficient drying, and the head, internal organs, skin, bones, etc. other than the fish portion may be removed. The moisture content after drying varies depending on the type of fish, but is preferably 45% by mass or less, and more preferably 30% by mass or less. (Hereinafter simply indicated as “%”)

As long as it is a general fish, any fish can be used, but Perciformes are preferable, and it is more preferable to select from Persian or Saba. Among them, it is most preferable to use a fish of the mackerel subfamily Sabaidae. Scombridae belongs to the genus Scomberomorus, Scomber genus Scomber, Soda genus Auxis, Suma genus Euthynnus, Skipjack genus Katsuwonus, Tuna genus Thunnus, etc., and use one or more of these fish in any combination It is possible.

Fish that is commercially available in a dry state may be used from the viewpoints of safety and availability. As an example of a commercial item, various dried foods, bran, kabushi, boiled dried fish, compressed dried dried fish, etc. are meant. Above all, with regard to bran and dried sardines, stable quality can be obtained by using products that conform to Japanese agricultural and forestry standards. According to Japanese Agricultural Standard Shavings, fish, bonito, mackerel, tuna and other fish are removed from their heads, internal organs, etc., solidified by ripening and then cooled to a moisture content of 26%. It is the one that has been dried so that it becomes the following. Kabushi means a mold with a mold (more than 2 molds). According to the regulations of Japanese Agricultural Standard Niboshi Fish, Niboshi means that fish such as sardines, horse mackerel, etc. are coagulated and dried to solidify the protein. Compressed-boiled dried food refers to a product obtained by solidifying a protein by boiling and then pressing the fish to remove fish oil and drying.

As the aqueous solvent used for the extraction of the present invention, water or a mixture of water and a water-soluble organic solvent is used. As the water-soluble organic solvent, one or more solvents that can be usually used for extraction can be freely selected and used. Examples thereof include alcohols, glycols, ketones, esters, ethers, and halogenated carbons. Examples of alcohols include ethanol, methanol, and propanol. Examples of glycols include ethylene glycol, diethylene glycol, butylene glycol, and propylene glycol. Examples of ketones include acetone and methyl ethyl ketone. Examples of the esters include ethyl acetate, propyl acetate, and ethyl formate. The water content in the mixture of water and the water-soluble organic solvent is 10% or more, preferably 50 to 90%. The pH of the aqueous solvent is preferably in the range of 1 to 12, preferably 4 to 10, and inorganic acids such as sulfuric acid, nitric acid, phosphoric acid and their salts, acetic acid, citric acid, malic acid, lactic acid, succinic acid The pH can be adjusted with an organic acid such as the above and a salt thereof, a hydroxide such as alkali metal or alkaline earth metal, or a basic substance such as ammonia or amine. Further, it may contain salts such as sodium chloride and magnesium chloride, and sugars such as sugar and glucose.

The extraction method is not particularly limited, and usually, an extraction method used for extracting soup stock or an extraction method used for extracting plant extract can be freely selected and used. For example, a method of extracting and filtering a dried fish after immersing the dried fish in the aqueous solvent, a method of pulverizing the dried fish and extracting it with a solvent in a high temperature and high pressure state, and the like can be mentioned.

The extract of the present invention can be used as a skin external preparation and a cosmetic ingredient. The dry solid content concentration of the dried fish extract according to the present invention can be freely set according to the type of extraction solvent used, the extraction method, etc., as long as the effects of the present invention are not impaired. In particular, 0.0001 to 5% is preferable, and 0.001 to 3% is more preferable. If it is less than 0.0001%, the effect of promoting ceramide production and the effect of moisturizing the skin may be weak. On the other hand, if it exceeds 5%, an unfavorable color, smell or precipitation may occur. Because.

The external preparation for skin and cosmetics containing the extract of the present invention has a ceramide production effect and an effect of moisturizing the extract from dried fish, so that the production amount of ceramide is maintained at a normal amount, and a high moisturizing effect and Has a barrier effect. The content of the extract in the external preparation for skin is preferably 0.00001 to 0.1%, more preferably 0.0001 to 0.001% as a solid content. If it exists in this range, it can mix | blend stably and can show the effect which smoothes the high skin roughness and the skin.

The cosmetics and external preparations for skin using the extract according to the present invention described above are highly likely to be administered to patients suffering from various diseases because their active ingredients are naturally derived ingredients. . Moreover, there is little need to worry about side effects even if administered continuously for a long period of time.

Ceramide is an N-acyl derivative of sphingosine or a similar base, and the chain length of the acyl group (in ceramide existing in the human body, the distribution of acyl chain length is said to be about C _{16 to} C ₂₄ ). There are different types. The ceramide production promoter of the present invention has an effect on all, regardless of the acyl chain length, and has a high promoting effect on a ceramide having an N-acyl group having a C _{16 to} C ₂₄ chain length, Particularly for ceramide having an N-acyl group having a chain length of C _{22 to} C _24, a high promoting effect can be obtained even at a low concentration.

The dried fish extract is formulated with a combination of one or more other medicinal agents, further enhancing the ceramide production promoting effect, or exhibiting other medicinal effects together with the ceramide production promoting effect and Cosmetics can be prepared. Examples of other medicinal agents include vitamins, antibacterial agents, anti-inflammatory agents, anti-acne agents, whitening agents, UV protection agents, hair restorers, cell activators, active oxygen scavengers, and moisturizers. However, it is not limited to these.

Examples of vitamins include vitamin B ₁ (thiamine), vitamin B ₂ (riboflavin), vitamin B ₃ (niacin), vitamin B ₅ (pantothenic acid), vitamin B ₆ (pyridoxal, pixidoxamine, pyridoxine), vitamin B ₇ (biotin) ), Vitamin B ₉ (folic acid), vitamin B ₁₂ (cyanocobalamin, hydroxocobalamin) and the like, and their derivatives, vitamin C (ascorbic acid) and its derivatives, vitamin A (retinol, retinal, retinoic acid, β-carotene, etc. Carotenoids) and derivatives thereof, vitamin D (ergocalciferol, cholecalciferol) and derivatives thereof, vitamin E (tocopherol, tocotrienol) and derivatives thereof, vitamin K (phylloquinone, menaquinone) And its derivatives, ergadenyl acid (adenylic acid), orotic acid, lipoic acid, linoleic acid, inositol, choline, carnitine, adenylthiomethylpentose, carnitine, quercetin, hesperidin, rutin, ubiquinone, nicotinamide adenine dinucleotide, methyl chloride Examples include vitamin-like substances such as methionine sulfonium.

Antibacterial agents include bactericides, antifungal agents, antiviral agents, and various antibiotics, benzoic acid and its salts, chlorhexidine gluconate, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

Anti-inflammatory agents are used for the purpose of suppressing inflammation such as hot flashes and erythema on the skin after sunburn, and for the treatment of dermatitis. Steroid hormones and derivatives thereof, indomethacin and derivatives thereof, azulene and derivatives thereof, sulfur and Its derivatives, Glycyrrhizic acid and its derivatives, Glycyrrhetinic acid and its derivatives, Aloe extract, Artea extract, Ashitaba extract, Arnica extract, Ginseng extract, Nettle extract, Oat extract, Hypericum extract, Chamomile extract , Goldfish extract, watercress extract, comfrey extract, salvia extract, sicon extract, perilla extract, birch extract, gentian extract, etc.

Anti-acne agents include various antibiotics, sulfur, salicylic acid, resorcin, vitamin A and its derivatives, benzoyl peroxide, and the like.

The whitening agent is used for the purpose of preventing the phenomenon of darkening of the skin caused by sunburn or the like, the stain caused by pigmentation, freckles, etc., kojic acid, arbutin, hydroquinone, ellagic acid, linoleic acid, vitamin C and its derivatives, AMP , Vitamin E and its derivatives, 4-methoxysalicylic acid potassium salt, glycyrrhizic acid and its derivatives, tranexamic acid, 4-n-butylresorcinol, biphenyl such as 5,5′-dioldipropyl-biphenyl-2,2′-diol Derivatives, placenta extract, chamomile extract, licorice extract, ages extract, oxon extract, seaweed extract, cucumber extract, caquette extract, gokahi extract, rice bran extract, wheat germ extract, saicin extract , Hawthorn extract, Sunpens extract, Shirayuri extract, Peonies Extracts, Sempukuka extract, soybean extract, tea extract, molasses extract, sandalwood extract, grape extract, hop extract, squid extract, mokka extract, yukinoshita extract, Yokuinin extract, etc. .

As UV protection agents, paramethoxycinnamic acid-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 sodium sulfate, 4-butyl-4'-methoxydibenzoylmethane 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, zinc oxide and the like.

Examples of the hair restorer include fennel extract, swellthianin, minoxidil, T-flavanone, 6-benzylaminopurine, pentadecanoic acid glyceride, ginger tincture, assembly extract, carrot extract, carpronium chloride, adenosine, finasteride and the like.

Cell activators are used for the purpose of improving rough skin, etc., such as caffeine, chicken crown extract, shell extract, shell extract, royal jelly, silk protein and its degradation product or derivatives thereof, lactoferrin or its degradation product, Mucopolysaccharides such as chondroitin sulfate and hyaluronic acid, or salts thereof, collagen, yeast extract, lactic acid bacteria extract, bifidobacteria extract, fermentation metabolic extract, ginkgo biloba extract, barley extract, assembly extract, tiso extract , Carrot extracts such as carrot extract, rosemary extract, carnitine and levocarnitine chloride, organic acids such as glycolic acid, citric acid, lactic acid, malic acid, tartaric acid and succinic acid, and derivatives thereof.

Active oxygen scavenger is used for the purpose of suppressing oxidative damage such as lipid peroxide production suppression, superoxide dismutase, mannitol, quercetin, catechin and its derivatives, rutin and its derivatives, button pi extract, yashajitsu extract, melissa extract , Extract of rahan fruit, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivative, riboflavin and its derivative, pyridoxine and its derivative, vitamin B such as nicotinic acid and its derivative, tocopherol and its derivative, etc. Vitamin E, dibutylhydroxytoluene, butylhydroxyanisole, etc. are mentioned.

As the humectant, proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, amino acids such as glycine, serine, aspartic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, trehalose, Inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipids, ceramide, auren extract, agate extract, agar extract, a senkyu extract, Examples of this include mallow mushroom extract, periwinkle extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

There are no particular limitations on the formulation of the external preparation for skin and cosmetics of the present invention. For example, emulsions, creams, lotions, cosmetics, packs, cleaning agents, makeup cosmetics, lotions, ointments, suspensions. It may be any form of cosmetics such as emulsions, aerosols, patches, and external medicines.

In the external preparation for skin of the present invention, components that are usually used in preparations such as cosmetics, quasi-drugs, and external medicines, that is, water, higher alcohols, are used as long as the effects of the present invention are not impaired. , Lower alcohols, polyhydric alcohols, waxes, fatty acids, oils, surfactants, metal soaps, gelling agents, powders, water-soluble polymers, film forming agents, resins, clathrate compounds, preservatives , Fragrances, deodorants, salts, pH adjusters, fresheners, animal / microbe-derived extracts, plant extracts, blood circulation promoters, astringents, chelating agents, keratolytic agents, enzymes, hormones, etc. it can.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.
[Extraction Example 1: Manufacture of cutlet extract]
A cutlet shaving (Japanese Agricultural Standards compliant product) was used. 40 g of katsuobushi shavings were mixed with 400 mL of water and heated at 95 ° C. for 1 hour. Then, it coarse-filtered with Saran filter cloth (Asahi Kasei company make), and concentrated with the centrifugal evaporator. Ethanol and water were added so that the final concentration of ethanol was 30% to obtain 24 mL of cutlet extract. Using this bonito extract as a stock solution, 1/3 and 1/10 dilution series with 30% ethanol were prepared.

[Extraction Example 2: Production of Anchovy Extract]
Niboshi (Japanese Agricultural Standards compliant product) made from anchovy was used. After crushing 20 g of boiled and dried, 200 mL of water was added and immersed overnight at room temperature. Furthermore, 200 ml of ethanol was added, extracted at room temperature for 1 hour, and coarsely filtered with Saran filter cloth (Asahi Kasei Co., Ltd.) to obtain an anchovy extract.

[Extraction Example 3: Production of horse mackerel extract]
84.9 g of Amaji Open Dried was used. After finely cutting with scissors, 200 mL of water and 200 mL of ethanol were added and extracted at room temperature for 1 hour. The filtrate which was roughly filtered with Saran filter cloth (manufactured by Asahi Kasei Co., Ltd.) was allowed to stand, and the floating oily insoluble matter was removed to obtain an aji extract.

[Experimental example 1: Ceramide production promotion test of bonito extract]
Normal human epidermal keratinocytes were seeded in a 10 cm petri dish containing Kurabo Industries' medium (HuMedia-KG2Ca 0.03 mM), and the dilution series of Extraction Example 1 was added at 23 μL / mL medium after 5 days of culture. After further culturing for 3 days, the bonito extract was again added at 23 μL / mL medium, and cultured for 4 days until it became overconfluent. After culturing, the cells were washed twice with PBS, and the cells were collected using a scraper.
The collected cells were dissolved in a lysis buffer (20 mM HEPES pH 7.4, 10 μg / ml protease inhibitor, 1 mM dithiothreitol, 1 mM EDTA, 1 mM sodium orthovanadate, 15 mM sodium fluoride, and 0.5 mM 4-deoxypyridoxine), and C17-S1P was used as an internal standard. In addition, lipids were extracted by the Bligh & Dyer method. Lipids were dissolved in 20% acetonitrile containing 0.1% TFA, and ceramide was quantified by LC-MS / MS for each acyl chain length. The results are shown in the following table. The ceramide production promotion rate in the following table is a value calculated by the following formula based on the production amount of ceramide II.
Ceramide production promotion rate (%) = (ceramide amount with addition of bonito extract) / (ceramide amount with addition of 30% ethanol) × 100

上記表に示す結果から、カツオブシ抽出液は、高いセラミド産生促進作用を示すことが理解できる。

From the results shown in the above table, it can be understood that the cutlet extract extracts show a high ceramide production promoting action.

[Experimental Example 2: Skin roughness improvement test for external preparation for skin]
An external preparation for skin having the composition shown in Table 2 was prepared and tested for use. Table 2 shows the results of evaluating the rough skin improvement effect in single use and continuous use.

(Production method)
A. Components (1) to (5) are mixed and dissolved.
B. Components (6) to (11) are mixed and dissolved.
C. A and B were mixed and uniformed to obtain a skin external preparation.

(Evaluation method of rough skin improvement effect after single use)
The results of five levels of evaluation for each evaluation item were averaged and evaluated based on evaluation criteria by 10 female professional panels in their 30s.
〔Evaluation item〕
Immediately after the moist sensation, maintaining a smooth and moist feeling on the skin [5-level evaluation]
Very good 5 points Somewhat good 4 points Good 3 points Normal 2 points Bad 1 point [Evaluation criteria]
◎: 4.0 points or more
○: 2.5 points or more and less than 4.0 points Δ: 1.5 points or more and less than 3.0 points × less than 1.5 points

(Evaluation method of rough skin improvement effect in continuous use)
70 men and women suffering from dry skin in their 20s and 60s during the winter were randomly assigned to be 10 people in each sample, and they were used twice a day in the morning and evening for 2 consecutive weeks, and each evaluation item was rated on a 5-point scale. The results were averaged and evaluated based on the evaluation criteria.
〔Evaluation item〕
Rough texture of dry feeling [5-level evaluation]
Obviously improved 3 points slightly improved 2 points slightly improved 1 point unchanged 0 points deteriorated -1 point [evaluation criteria]
◎: 2.5 points or more ○: 1 point or more and less than 2.5 points △: 0 points or more and less than 1 point ×: Less than 0 points

As shown in Table 2, Examples 1 to 6 were excellent in moist feeling and moist feeling, and had an effect of improving rough skin. In contrast, the comparative example was inferior to the example.

[Example 7: lotion]
A lotion having the following composition was prepared by the following production method.
(Composition) (%)
(1) Retinol palmitate 0.005
(2) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
(3) Ethyl alcohol 15.0
(4) Preservative 0.1
(5) Perfume appropriate amount (6) Cutlet extract * 1 2.0
(7) D-panthenol 0.1
(8) 1,3-butylene glycol 4.0
(9) Citric acid 0.1
(10) Sodium citrate 0.25
(11) Purified water remaining amount * 1 Produced in Extraction Example 1 (Production method)
A. Components (1) to (6) are mixed and dissolved.
B. Components (7) to (11) are mixed and dissolved.
C. A and B were mixed and uniformed to obtain a skin lotion.

[Example 8: Cosmetic liquid]
A cosmetic liquid having the following composition was prepared by the following production method.
(Composition) (%)
(1) Polyoxyethylene (20E.O.) sorbitan 1.2
Monolaurate (2) Ethyl alcohol 7.0
(3) Horse mackerel extract * 2 0.5
(4) Yokuinin extract 0.2
(5) Ascorbic acid glucoside * 3 0.2
(6) Glycerin 5.0
(7) 1,3-butylene glycol 7.5
(8) Preservative 0.1
(9) Sodium alginate 1.0
(10) Fragrance Appropriate amount (11) Purified water Remaining amount * 2 Produced in Extraction Example 3
* 3 Hayashibara Biochemical Research Institute (manufacturing method)
A. Components (1) to (4) are mixed and dissolved.
B. Components (5) to (11) are mixed and dissolved.
C. A and B were mixed and uniformed to obtain a serum.

[Example 9: Latex]
An emulsion having the following composition was prepared by the following production method.
(Composition) (%)
(1) Polyoxyethylene (10E.O.) sorbitan 1.0
Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5
Tetraoleate (3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 8.0
(7) Astaxanthin * 4 0.01
(8) Vitamin E linoleate 0.05
(9) Carboxyvinyl polymer 0.1
(10) Sodium hydroxide 0.05
(11) Ethyl alcohol 5.0
(12) Cutlet extract * 1 0.00035
(13) Preservative 0.1
(14) Perfume appropriate amount (15) Purified water remaining * 4 Sigma (manufacturing method)
A. Ingredients (1) to (8) and (13) are heated and mixed and maintained at 70 ° C.
B. Ingredients (9) to (12) and (15) are heated and mixed and maintained at 70 ° C.
C. A is added to B, mixed and uniformly emulsified.
D. After cooling C, (14) was added and mixed uniformly to obtain an emulsion.

[Example 10: Cream]
A cream having the following composition was prepared by the following production method.
(Composition) (%)
(1) Polyoxyethylene (40E.O.) monostearate 2.0
(2) Glycerin monostearate (self-emulsifying type) 5.0
(3) Stearic acid 5.0
(4) Behenyl alcohol 0.5
(5) Squalane 15.0
(6) Cetyl isooctanoate 5.0
(7) Tocopherol acetate 1.5
(8) Anchovy extract * 5 0.0003
(9) Stearyl glycyrrhetinate 0.2
(10) 1,3-butylene glycol 5.0
(11) Vaseline 3.5
(12) Perfume appropriate amount (13) Preservative 0.1
(14) Purified water remaining amount * 5 Produced in Extraction Example 2 (Production method)
A. Components (1) to (7) and (9), (11) and (13) are heated and dissolved at 70 ° C.
B. Ingredients (8), (10), (14) are heated to 70 ° C.
C. Add A to B and allow to cool.
D. Ingredient (12) was added to C to obtain a cream.

[Example 11: Sunscreen]
A sunscreen having the following composition was prepared by the following production method.
(Composition) (%)
(1) Polyoxyethylene (10E.O.) sorbitan 1.0
Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5
Tetraoleate (3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 3.0
(7) 2-Methoxycinnamic acid-2-ethylhexyl 5.0
(8) 4-t-Butyl-4'-methoxy-dibenzoylmethane 7.5
(9) Horse mackerel extract * 2 0.0003
(10) Dipotassium glycyrrhizinate 0.1
(11) L-ascorbic acid magnesium phosphate 2.0
(12) Carboxyvinyl polymer 0.1
(13) Sodium hydroxide 0.05
(14) Ethyl alcohol 5.0
(15) Preservative 0.1
(16) Perfume appropriate amount (17) Purified water remaining amount (manufacturing method)
A. Ingredients (1) to (8) and (15) are heated and mixed and maintained at 70 ° C.
B. Ingredients (9) to (14) and (17) are heated and mixed and maintained at 70 ° C.
C. A is added to B, mixed and uniformly emulsified.
D. After cooling C, (16) was added and mixed uniformly to obtain a sunscreen.

[Example 12: Pack]
A pack having the following composition was prepared by the following production method.
(Composition) (%)
(1) Polyvinyl alcohol 20.0
(2) Ethyl alcohol 20.0
(3) Glycerin 5.0
(4) Kaolin 6.0
(5) Cutlet extract * 1 0.05
(6) Pyridoxine hydrochloride 0.05
(7) Perilla extract 1.0
(8) Preservative 0.2
(9) Fragrance 0.1
(10) Purified water remaining amount (manufacturing method)
A. Ingredients (1), (3) and (10) are mixed, heated to 70 ° C. and stirred.
B. Components (2), (4), (5) and (8) are mixed.
C. The above B was added to the above A, mixed and then cooled to uniformly disperse (6), (7) and (9) to obtain a pack.

[Example 13: Liquid foundation]
A liquid foundation having the following composition was prepared by the following production method.
(Composition) (%)
(1) Lanolin 7.0
(2) Liquid paraffin 5.0
(3) Stearic acid 2.0
(4) Cetanol 1.0
(5) Glycerin 5.0
(6) Triethanolamine 1.0
(7) Carboxymethylcellulose 0.7
(8) Purified water remaining amount (9) Mica 15.0
(10) Talc 6.0
(11) Titanium oxide 3.0
(12) Color pigment 6.0
(13) Anchovy extract * 5 0.0003
(14) Yukinoshita extract 0.05
(15) Perfume appropriate amount (production method)
A. Ingredients (1) to (5) are mixed and heated to keep at 70 ° C.
B. Add components (9) to (12) to A and mix uniformly.
C. Ingredients (6) to (8) are uniformly dissolved and maintained at 70 ° C.
D. C is added to B and emulsified uniformly.
E. After cooling D, components (13) to (15) were added to obtain a liquid foundation.

  All of these were applied to the skin, so that the amount of ceramide produced was maintained at a normal level, and beautiful skin having a high moisturizing effect and barrier effect was obtained.

  ADVANTAGE OF THE INVENTION According to this invention, it has the novel rough skin improvement effect and the ceramide production promotion effect, and can provide the skin external preparation and cosmetics with favorable safety | security and stability.

Claims (4)

An agent for improving rough skin, characterized by comprising, as an active ingredient, an extract obtained by extracting a fish portion of a dried periwinkle fish with an aqueous solvent, to be added to a skin external preparation or cosmetic . A ceramide production promoter comprising as an active ingredient an extract obtained by extracting a fish portion of a dried periwinkle fish with an aqueous solvent for addition to a skin external preparation or cosmetic . A skin external preparation containing the agent according to claim 1 or 2. Cosmetics containing the agent of Claim 1 or 2.