KR102577747B1 - Cosmetic composition comprising champsocephalus gunnari extract or notothenia rossii extract as an active ingredient for improving wrinkles - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles containing an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled blackfish ( Notothenia rossii ) extract as an active ingredient. The Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or the Antarctic marbled blackfish ( Notothenia rossii ) extract of the present invention not only has excellent collagen biosynthesis enhancement and MMP-1 inhibitory activity even at very low concentrations, but also protects cells from ultraviolet (UV) rays. Since it has the following effect, a composition containing it as an active ingredient can be usefully used as a functional cosmetic composition for wrinkle improvement. In particular, the extract of the present invention is a natural material derived from Antarctic fish, and the cosmetic composition of the present invention containing it has the advantage of being safe even for long-term use.

Description

Cosmetic composition for improving skin wrinkles comprising Antarctic Meckel smelt extract or Antarctic marbled blackfish extract as an active ingredient {Cosmetic composition comprising champsocephalus gunnari extract or notothenia rossii extract as an active ingredient for improving wrinkles}

The present invention relates to a cosmetic composition for improving skin wrinkles containing an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled blackfish ( Notothenia rossii ) extract as an active ingredient.

The skin is in direct contact with the external environment and has the function of protecting it from temperature, humidity, antigens, and ultraviolet rays. However, as skin function deteriorates due to physical and chemical stimulation such as external pollutants, ultraviolet rays, and stress, cell proliferation and immune activity weaken as we age, preventing rapid repair of skin cell damage and overall deterioration of skin components. This causes skin wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into intrinsic aging and extrinsic aging. Intrinsic aging is a naturally occurring aging phenomenon that is clinically characterized by decreased elasticity, rough skin texture, deep wrinkles, and pigmentation. Extrinsic aging is an aging phenomenon caused by external environmental factors such as ultraviolet rays, reactive oxygen species, and stress. Recently, the amount of UV has increased due to rapid industrialization and the resulting air pollution and destruction of the ozone layer, which leads to active aging. It generates powerful oxidizing substances such as oxygen species, causing destruction of connective tissue formation such as skin collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein function, and modification of intracellular signaling substances, resulting in the creation of skin wrinkles and pigmentation in humans. This causes skin aging. To prevent such skin damage, cosmetics or skin protectants containing various skin protection ingredients such as retinol, retinoid, vitamin C, flavonoid and tocopherol, and coenzyme Q10 are being developed.

In particular, ultraviolet rays (UV) from sunlight are one of the well-known causes of aging. Skin exposed to UV rays for long periods of time has a thickened stratum corneum and collagen and elastin, the main components of the skin, are denatured, causing the skin to lose its elasticity. Collagen and elastin are regulated by various factors. Due to the expression of matrix metallo proteases such as collagenase and elastase, the collagen and elastin produced are decomposed, resulting in A decrease in collagen content occurs. Various substances have been developed and used for the purpose of suppressing the decrease in collagen and elastin, which are the causes of this decrease in elasticity. Among them, retinol and retinoic acid show an elasticity improvement effect, and protein fraction obtained from Leguminosae Seeds. It is also used to show the effect of increasing elasticity. However, these retinoids have the disadvantage of being sensitive to skin irritation and allergies even if only a small amount is applied to the skin.

Recently, in order to develop relatively low-toxic and nature-friendly cosmetics, biologically active substances such as whitening, anti-aging, anti-wrinkle, antioxidant, and anti-inflammatory properties have been explored through extraction and fermentation technology using natural products, and then used as cosmetic materials. I am using it.

Meanwhile, Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled smelt ( Notothenia rossii ) is a marine biological resource that lives only in Antarctica.

Accordingly, while the present inventors were seeking to find a natural substance that has no side effects on the living body and is excellent in improving skin wrinkles, they focused on Antarctic fish and tested its pharmacological effects. As a result, they found Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled blackfish ( In the case of the extract extracted from (Notothenia rossii ), it not only has an excellent effect of increasing collagen biosynthesis and MMP-1 inhibitory activity at very low concentrations, but also has an effect of protecting cells from ultraviolet rays (UVB), making it functional for improving skin wrinkles. The present invention was completed by confirming that it has sufficient value as a cosmetic material.

Korean Patent Publication No. 10-2020-0104467 Korean Patent No. 10-2018-0008557

Therefore, the purpose of the present invention is to provide a cosmetic composition that can effectively improve skin wrinkles.

In order to achieve the purpose of the present invention as described above,

The present invention provides a cosmetic composition for improving skin wrinkles containing Antarctic Meckel smelt (Champsocephalus gunnari ) extract or Antarctic marbled blackfish ( Notothenia rossii ) extract as an active ingredient.

In one embodiment of the present invention, the extract may be extracted with one or more solvents selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, water, and hexane.

In one embodiment of the present invention, the extract may have an effect of increasing collagen biosynthesis and MMP-1 inhibitory activity.

In one embodiment of the present invention, the extract may have the effect of protecting cells from ultraviolet rays.

In one embodiment of the present invention, the cosmetic composition includes soft lotion, gel, water-soluble liquid, milk lotion, nutritional cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, and solid anhydrous product. , oil dispersion in aqueous phase using globules, ionic lipid vesicles, non-ionic lipid vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water type makeup base, water-in-oil type makeup base, foundation, skin It may be one type of formulation selected from the group consisting of cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

The Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or the Antarctic marbled blackfish ( Notothenia rossii ) extract of the present invention not only has excellent collagen biosynthesis enhancement and MMP-1 inhibitory activity even at very low concentrations, but also protects cells from ultraviolet (UV) rays. Since it has the following effect, a composition containing it as an active ingredient can be usefully used as a functional cosmetic composition for wrinkle improvement. In particular, the extract of the present invention is a natural material derived from Antarctic fish, and the cosmetic composition of the present invention containing it has the advantage of being safe even for long-term use.

Figure 1 is a graph showing the MMP-1 inhibitory activity of the Antarctic fish extract (25 μg/ml) of the present invention (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
Figure 2 is a graph showing the effect of the Antarctic fish extract (25 μg/ml) of the present invention to increase collagen biosynthesis (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6 : NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
Figure 3 shows the results of measuring cell viability according to treatment with Antarctic fish extract (25 μg/ml) of the present invention after UVB (125 mJ/cm ² ) irradiation (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).
Figure 4 shows the results of measuring cell viability according to treatment with Antarctic fish extract (25 μg/ml) of the present invention after UVB (144 mJ/cm ² ) irradiation (1: CG skin, 2: CG brain, 3: CG liver, 4: CG stomach, 5: NR skin, 6: NR brain, 7: NR liver, 8: NR stomach, CG: Champsocephalus gunnari , NR: Notothenia rossii ).

The present invention is characterized by providing a cosmetic composition for improving skin wrinkles containing an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled blackfish ( Notothenia rossii ) extract as an active ingredient.

The “Antarctic Mekel smelt extract or Antarctic marbled grayfish extract” of the present invention is directly extracted and isolated from Antarctic Mekel smelt ( Champsocephalus gunnari ) or Antarctic marbled blackfish ( Notothenia rossii ) using extraction and separation methods known in the art. The obtained product can be used, and the 'extract' defined in the present invention is extracted from various parts such as the skin, brain, liver, and stomach of the Antarctic fish using an appropriate solvent. It is extracted, and includes, for example, crude extracts, polar solvent-soluble extracts, or non-polar solvent-soluble extracts of various parts of the Antarctic fish (skin, brain, liver, stomach).

The appropriate solvent for extracting the Antarctic Meckel smelt extract or the Antarctic marbled blackfish extract of the present invention may be any pharmaceutically acceptable organic solvent, and water or an organic solvent may be used, but is not limited thereto. , for example, alcohols having 1 to 4 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, butanol, etc., acetone, Various solvents such as ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane can be used individually or in combination. You can. Preferably, hexane is used.

As an extraction method, any one of the following methods can be selected and used: hot water extraction, cold needle extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation to the method for producing the extract of the present invention, and any known method can be used.

For example, the Antarctic Meckel smelt extract or the Antarctic marbled blackfish extract of the present invention is obtained by converting the primary extract extracted by the above-mentioned hot water extraction or solvent extraction method into a powder state by additional processes such as reduced pressure distillation and freeze-drying or spray drying. It can be manufactured. In addition, the primary extract was further purified into fractions using various chromatographies such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. You can also get it.

Therefore, in the present invention, Antarctic Meckel smelt extract or Antarctic marbled blackfish extract is a concept that includes all extracts, fractions, and purifications obtained at each stage of extraction, fractionation, or purification, as well as their dilutions, concentrates, or dried products.

In one embodiment of the present invention, the extract can be extracted by freezing and drying the skin, brain, liver, and stomach tissues of Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled blackfish ( Notothenia rossii ), then grinding them, and adding hexane. there is.

The present inventors tested that Antarctic Meckel smelt extract or Antarctic marbled blackfish extract has an effect of increasing collagen biosynthesis and MMP-1 inhibitory activity (see Experimental Example 1), and has a cytoprotective effect according to UVB irradiation (see Experimental Example 2). It was proven through .

The composition of the present invention may include Antarctic Meckel smelt extract or Antarctic marbled blackfish extract, as well as ingredients commonly used in cosmetic compositions, such as conventional antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. It may contain phosphorus adjuvants and carriers.

In addition, the composition of the present invention, in addition to the above-described active ingredients (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract), can also be used by mixing conventionally used skin protectants as long as the skin protection effect is not impaired by reaction with the active ingredients. there is.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nourishing lotion, various creams, essences, packs, foundations, cleansing products, face washes, soaps, treatments, and serums. etc.

Specific formulations of the cosmetic composition of the present invention include soft lotion, gel, water-soluble liquid, milk lotion, nutritional cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, solid anhydrous product, and spherules. Oil dispersion in aqueous phase, ionic lipid vesicles, non-ionic lipid vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water type makeup base, water-in-oil type makeup base, foundation, skin cover, lipstick, It includes one type of formulation selected from the group consisting of lip gloss, face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

According to a preferred embodiment of the present invention, the content of the active ingredient of the present invention (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) is 0.0001-20% by weight, preferably 0.5-20%, based on the total weight of the composition, More preferably, it is 1.0-20% by weight. If the content of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) is less than 0.0001% by weight, the wrinkle improvement effect is greatly reduced, and if it exceeds 20% by weight, it may cause skin irritation, and problems with formulation. This can happen.

Meanwhile, the cosmetic composition according to the present invention may be formulated by stabilizing the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) contained within nanoliposomes. When the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) is contained inside the nanoliposome, the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) is stabilized, causing precipitation formation, discoloration, and discoloration during formulation. Problems such as off-odor can be solved, and the solubility and transdermal absorption of ingredients can be increased, thereby maximizing the efficacy expected from the extract.

In the present invention, nanoliposome refers to a liposome having the form of a typical liposome and having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of nanoliposomes is 50 to 300 nm. When the average particle diameter of nanoliposomes exceeds 300 nm, improvement in skin penetration and formulation stability among the technical effects sought to be achieved in the present invention are very weak.

Nanoliposomes used to stabilize the extract according to the present invention can be prepared by a mixture containing polyol, oily component, surfactant, phospholipid, fatty acid and water.

The polyol used in the nanoliposome of the present invention is not particularly limited, and is preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, pentylene glycol, erythritol, and xylitol. , sorbitol, and mixtures thereof. The amount used is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of nanoliposomes.

The oil component used in the production of nanoliposomes of the present invention can be various oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil, ester-based synthetic oil, Silicone oils such as dimethicone and cyclomethicone, animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oil, and propoxylated alkyl ether oil. , phytosphingosine, sphingonoid lipids such as sphingosine and sphinganine, cerebroside cholesterol, sitosterol cholesteryl sulfate, cytosteryl sulfate, C10-40 fatty alcohols, and mixtures thereof. The amount used may be 1.0 to 30.0% by weight, preferably 3.0 to 20.0% by weight, based on the total weight of nanoliposomes.

The surfactant used in the production of nanoliposomes of the present invention can be any one known in the art. For example, anionic surfactants, cationic surfactants, amphoteric surfactants, and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkylacylglutamates, alkylphosphates, alkyllactylates, dialkylphosphates, and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters, and sugar esters. Particularly preferred surfactants are polysorbates, which belong to the class of nonionic surfactants. The amount used may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of nanoliposomes.

Phospholipids, another component used in the production of nanoliposomes of the present invention, are amphiphilic lipids, including natural phospholipids (e.g., egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (e.g., Dipalmitoylphosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, natural unsaturated or saturated lecithin extracted from soybeans or egg yolk is preferred. Typically, naturally derived lecithin contains 23-95% phosphatidylcholine and 20% or less phosphatidylethanolamine. In the production of nanoliposomes of the present invention, the amount of phospholipids used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight, based on the total weight of nanoliposomes.

The fatty acid used in preparing nanoliposomes of the present invention is a higher fatty acid, preferably a saturated or unsaturated fatty acid of a C12-22 alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, and linoleic acid. Includes. The amount used may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of nanoliposomes.

The water used in the production of nanoliposomes of the present invention is generally deionized distilled water, and the amount used may be 5.0 to 40% by weight based on the total weight of nanoliposomes.

Nanoliposomes can be produced through various methods known in the art, but are most preferably produced by applying a mixture containing the above components to a high pressure homogenizer. The production of nanoliposomes using a high-pressure homogenizer can be performed under various conditions (e.g., pressure, number of times, etc.) depending on the desired particle size, and is preferably performed 1 to 5 times under a pressure of 600 to 1200 bar. Nanoliposomes can be produced by passing through .

The cosmetic composition for improving skin wrinkles according to the present invention may contain 0.1 to 20.0% by weight of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled blackfish extract) based on the weight of nanoliposomes.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.

<Example 1>

Preparation of Antarctic fish extract of the present invention

Five frozen Antarctic fish Notothenia rossii (hereinafter abbreviated as 'NR') and Champsocephalus gunnari (hereinafter abbreviated as 'CG') each were thawed. In the case of NR, the fish were collected directly from the coast of the Sejong Science Station in Antarctica, and in the case of CG, by-caught fish were received from a deep-sea fishing company that fishes krill. Each individual was dissected to obtain tissue samples (skin, brain, liver, and stomach), and each area was washed with distilled water and freeze-dried. From the dried tissue samples, NR: 13.20 g of skin, 0.35 g of brain, 2.74 g of liver, 3.12 g of stomach, and CG: 8.50 g of skin, 0.22 g of brain, 8.36 g of liver, and 2.78 g of stomach were obtained. Each sample was crushed into small pieces using a mortar and pestle, transferred to each 200 mL glass bottle, then 200 mL of 95% hexane was added, and extraction was repeated twice a day at room temperature (24 hr Each extract obtained through the above process was transferred to a round flask and the solvent was completely removed using a reduced pressure concentrator. As a concentrated extract with the solvent removed, NR: 0.18 g of skin, 0.13 g of brain, 0.24 g of liver, 0.21 g of stomach, CG: 0.41 g of skin, 0.08 g of brain, 1.78 g of liver, 0.52 g of stomach were finally obtained. An experiment was conducted using

Antarctic fish extract of the present invention extract sample Extraction solvent Pisces group One CG skin hexane 2 brain hexane 3 liver hexane 4 stomach hexane 5 NR skin hexane 6 brain hexane 7 liver hexane 8 stomach hexane

<Experimental Example 1>

Wrinkle improvement effect of Antarctic fish extract of the present invention

In this experiment, the skin wrinkle improvement effect of the Antarctic fish extract of the present invention prepared through <Example 1> was examined.

<1-1> Measurement of MMP-1 inhibitory activity

The skin wrinkle improvement effect was evaluated by the inhibitory effect on matrix metalloproteinase-1 (hereinafter abbreviated as ‘MMP-1’), one of the collagenase enzymes that decomposes and destroys collagen.

In detail, normal human dermal fibroblasts (hereinafter simply abbreviated as 'NHDF') were treated with UVB 125 mJ/cm ² and then a total of 8 types of Antarctic fish prepared through <Example 1> above. The extract was treated at a concentration of 25 μg/ml and cultured for a total of 72 hours, then cell medium was collected from each well, and MMP-1 was assayed using conditioned medium using a commercially available ELISA kit (Human Total MMP-1 kit). The concentration was analyzed three times for each sample.

As a result, as shown in Figure 1 and Table 2, MMP-1 was significantly increased when treated with UVB, and MMP-1 increased by UVB treatment was significantly increased by treatment with the eight types of Antarctic fish extract of the present invention. It was confirmed that it decreased.

Concentration of MMP-1 (pg/ml) according to Antarctic fish extract treatment of the present invention NCtr
(UVB-) Ctr
(UVB+) CG skin CG brain CG Liver CG above NR skin NR brain NR liver Above NR 3474.21 4746.48 2925.85 3261.02 3295.71 3216.54 2995.63 3091.67 2727.64 3310.56

<1-2> Measurement of collagen biosynthesis level

Collagen is synthesized into procollagen within cells, and is then secreted to the outside of the cell and polymerized into collagen fibers. Collagen is a structural protein that makes up the skin, and when the collagen content in the skin is high and the activity of collagen decomposing enzyme is low, wrinkles on the skin are improved. In this experiment, in order to examine the effect of improving skin wrinkles, the degree of collagen biosynthesis was confirmed by measuring Procollagen type-IC peptide (hereinafter abbreviated as ‘PIP’).

In detail, NHDF was treated with UVB 144 mJ/cm ² and then treated with a total of 8 types of Antarctic fish extracts prepared through <Example 1> at a concentration of 25 μg/ml, cultured for a total of 72 hours, and then cultured in each well. Cell medium was collected, and the amount of PIP was analyzed three times for each sample using conditioned medium using a commercially available ELISA kit (R&D Systems Inc.; Procollagen).

As a result, as shown in Figure 2 and Table 3, it was confirmed that the amount of PIP was significantly reduced when treated with UVB, and when the Antarctic fish extract of the present invention was treated after UV irradiation, extract 5 (NR skin), extract 7 The experimental group treated with extract 8 (NR liver) and extract 8 (NR stomach) showed a significantly increased amount of PIP.

Amount of PIP (ng/ml) according to Antarctic fish extract treatment of the present invention NCtr
(UVB-) Ctr
(UVB+) CG skin CG brain CG Liver CG above NR skin NR brain NR liver Above NR 454.33 297.67 240.83 255.50 193.83 246.25 485.58 271.33 533.10 505.83

<Experimental Example 2>

Cell protection effect of the Antarctic fish extract of the present invention

In this experiment, in order to determine the cell protection effect of a total of eight types of Antarctic fish extract prepared in <Example 1> according to UVB irradiation, the cell viability was examined through MTT analysis.

NHDF (Normal Human Dermal Fibroblasts) cells, a human skin fibroblast cell line, are exposed to UVB 125 mJ/cm ² or After treatment at 144 mJ/cm ² , a total of 8 types of Antarctic fish extracts prepared in <Example 1> were treated at a concentration of 25 μg/ml and cultured for a total of 72 hours. Afterwards, the medium was removed and 200 μL of 1 mg/mL MTT solution was added to each well, followed by reaction at 37°C for 2 hours in the presence of 5% CO ₂ . Afterwards, the substrate-containing medium was removed and 200 μL of DMSO was added to each well to dissolve the formazan crystals. The plate was shaken on an orbital shaker for 10 minutes at room temperature. The absorbance of a 100 μL aliquot of formazan dissolved in DMSO was quantified by measuring the optical density (OD) at 570 nm using an ELISA reader (Molecular Devices E09090, San Francisco, CA, USA).

As a result, as shown in Figure 3, it was confirmed that the cell survival rate (%) decreased by about 25% due to UVB (125 mJ/cm ² ) irradiation, and when the Antarctic fish extract of the present invention was treated after UV irradiation, the extract Except for extract 1 (CG Skin) and extract 6 (NR Brain), it was confirmed that cell viability (%) increased compared to UVB irradiation alone. In particular, the experimental group treated with extract 2 (CG brain), extract 4 (CG above), and extract 8 (NR above) showed significantly increased cell viability (%), which showed that among the Antarctic fish extracts of the present invention, In the case of extract 2 (CG brain), extract 4 (CG above), and extract 8 (NR above), it was determined that the concentration of 25 μg/ml would be effective in protecting cells from ultraviolet rays.

In addition, as shown in Figure 4, it was confirmed that the cell survival rate (%) decreased by about 25% due to UVB (144 mJ/cm ² ) irradiation, and when the Antarctic fish extract of the present invention was treated after UV irradiation, all It was confirmed that cell survival rate (%) increased in the experimental group compared to UVB irradiation alone.

Through the above results, it was confirmed that the Antarctic fish extract of the present invention has a cytoprotective effect due to UVB irradiation and is not cytotoxic.

<Experimental Example 3>

Fatty acid analysis of Antarctic fish extract of the present invention

For fatty acid analysis, lipids were extracted according to the method of Folch et al. (J. Folch, M. Lees, G. H. S. Stanley. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 497-509 .).

Briefly, it was methyl esterified with boron trifluoride-methanol solution (BF3-MeOH, 14%) and analyzed by gas chromatography (Varian (3400) / Shimadzu (GC-17A)), and the identification of fatty acids was performed under the same conditions. The peak area ratio was calculated by comparing with the retention time of the standard product. Analysis was performed using Omegawax 250 (0.25 mm The condition of split 100:1 was used.

The results are shown in detail in Table 4 below.

Fatty acid names and symbols Carbon number: Double bond number Fatty acid name Yeongmyeong (official name) English name (family name) 12:00 Lauric acid Lauric acid Dodecanoic acid 14:00 Myristic acid Myristic acid Tetradecanoic acid 16:00 palmitic acid Palmitic acid Hexadecanoic acid 18:00 stearic acid Stearic acid Octadecanoic acid 20:00 Arachidic acid Arachidic acid Eicosanoic acid 14:01 Myristoleic acid Myristoleic acid Tetradecenoic acid 16:01 palmitoleic acid Palmitoleic acid Hexadecenoic acid 18:01 oleic acid Oleic acid Octadecenoic acid 20:01 Eicosenoic acid Eicosenoic acid Eicosenoic acid 22:01 Erucic acid (docosenic acid) Erucic acid Docosenoic acid 18:2n-6 linoleic acid Linoleic acid 9,12-Octadecadienoic acid 18:3n-3 Alpha-linolenic acid α-Linolenic acid 9,12,15-Octadecatrienoic acid 18:4n-3 stearidonic acid Stearidonic acid 6,9,12,15-Octadecatetraenoic acid 20:4n-6 Arachidonic acid Arachidonic acid 5,8,11,14-Eicosatetraenoic acid 20:5n-3 Eicosapentaenoic acid Eicosapentaenoic acid 5,8,11,14,17-Eicosapentaenoic acid 22:5n-3 Docosapentaenoic acid Docosapentaenoic acid 7,10,13,16,19-Docosapentaenoic acid 22:6n-3 Docosahexaenoic acid Docosahexaenoic acid 4,7,10,13,16,19-Docosahexaenoic acid

Formulation Example 1: Soft lotion (skin lotion)

As shown in the table below, softening lotion was prepared according to a conventional method.

Formulation example of soft lotion of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1~5 1.3-Butylene glycol 6.0 glycerin 4.0 DL-panthenol 0.3 allantoin 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Fragrance, preservative a very small amount Purified water to 100

Formulation example 2: Nutritional lotion (milk lotion)

As shown in the table below, nutritional lotion was prepared according to a conventional method.

Formulation example of nutritional lotion of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1~5 Glyceryl Stearate SE 1.5 Cetearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Solvitastearate 0.5 hydrogenated vegetable oil 4.0 mineral oil 5.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopherol acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium Hyaluronate 5.0 Triethanolamine 0.12 Uniside-U 13 0.02 incense a very small amount Distilled water to 100

Formulation Example 3: Nutritional Cream

As shown in the table below, nutritional cream was prepared according to a conventional method.

Formulation example of nutritional cream of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1~5 Lipophilic glycerin monostearate 1.5 Cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Solvitastearate 0.6 Isostearyl Isostearate 5.0 squalane 5.0 mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Uniside-U13 0.02 incense a very small amount Distilled water to 100

Formulation Example 4: Massage Cream

As shown in the table below, massage cream was prepared according to a conventional method.

Formulation example of massage cream of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1~5 propylene glycol 6 glycerin 4.0 Triethanolamine 0.5 beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl alcohol 2.0 liquid paraffin 30.0 Carboxy vinyl polymer 0.5 incense a very small amount Distilled water to 100

Formulation Example 5: Pack

As shown in the table below, the pack was prepared according to a conventional method.

Pack formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1~5 glycerin 10.0 Betaine 5.0 REG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate 80. Carboxy vinyl polymer 0.2 Triethanolamine 0.18 octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 Uniside-U13 0.01 incense a very small amount Distilled water to 100

So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.

Claims (5)

A cosmetic composition for improving skin wrinkles comprising Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or Antarctic marbled blackfish ( Notothenia rossii ) extract as an active ingredient. According to paragraph 1,
A cosmetic composition for improving skin wrinkles, characterized in that the extract is extracted with one or more solvents selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, water, and hexane. According to paragraph 1,
The extract is a cosmetic composition for improving skin wrinkles, characterized in that it has a collagen biosynthesis enhancing effect and MMP-1 inhibitory activity. According to paragraph 1,
A cosmetic composition for improving skin wrinkles, characterized in that the extract has the effect of protecting cells from ultraviolet rays. According to any one of claims 1 to 4,
The cosmetic composition includes soft lotion, gel, water-soluble liquid, milk lotion, nourishing cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, solid anhydrous product, oil in aqueous phase using spherules. Dispersion, ionic lipid vesicles, non-ionic lipid vesicles, ointment, cleansing foam, cleansing water, pack, body oil, oil-in-water type makeup base, water-in-oil type makeup base, foundation, skin cover, lipstick, lip gloss, face powder A cosmetic composition for improving skin wrinkles, characterized in that it is a type of formulation selected from the group consisting of two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.