JP2003171256A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation comprising collagen extracted from mucopolysaccharide and fishes. <P>SOLUTION: This skin care preparation is excellent in action for improving chapped skin and fine wrinkles and applicable to cosmetics, medicines, quasi- drugs, etc. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin, and more particularly to an external preparation for skin such as cosmetics, pharmaceuticals, quasi-drugs, etc., which has an excellent effect of improving rough skin and fine wrinkles.

[0002]

2. Description of the Related Art Conventionally, external skin preparations for improving rough skin have been known, and the medicinal components to be added to them are allantoin, aloe extract, ginseng extract, placenta extract, and bovine blood removal. Proteins, fermentation metabolites, etc. are known.

It is also known that deterioration of proteins such as collagen and a decrease in production function are caused in the body due to aging, ultraviolet rays, constipation due to irregular life, nutritional deficiency and the like. Therefore, due to its excellent applicability to the human body, collagen extracted from cows and pigs has been applied to artificial skin, artificial blood vessels and the like, and has been added to cosmetics for the purpose of improving fine wrinkles.

[0004]

However, the above-mentioned external preparation for skin containing a medicinal component for the purpose of improving rough skin does not exhibit a sufficient effect in practice, and has a superior effect for improving rough skin. Development was desired.

Further, it is difficult for a skin external preparation containing collagen extracted from cattle or pigs for the purpose of improving fine wrinkles to exert its effect sufficiently with collagen alone.
In addition, it was necessary to add a large amount to achieve the purpose.

[0006] Furthermore, products containing collagen extracted from cows have some consumers who are uneasy due to bovine spongiform encephalopathy (BSE) and cannot be used due to religious reasons. As for products containing collagen extracted from the above, there are consumers who cannot use it due to religious reasons, and thus collagen that can be replaced with cattle or pigs is desired.

[0007]

Under these circumstances, the inventors of the present invention have conducted extensive studies and as a result, as a result, the compositions containing the specific components described below are combined, and the composition containing them has an excellent effect of improving rough skin and fine wrinkles. The present invention has been completed by discovering that it can be used as an external preparation for skin.

That is, the present invention provides a skin external preparation characterized by containing the following components (A) and (B); (A) mucopolysaccharide (B) collagen extracted from fish.

The mucopolysaccharide of component (A) used in the present invention is not particularly limited as long as it is a mucopolysaccharide, but a polysaccharide constituting hexosamine, such as chondroitin sulfate,
Examples thereof include dermatan sulfate, heparan sulfate, keratan sulfate and chitin, and salts thereof such as sodium chondroitin sulfate, sodium dermatan sulfate, sodium heparan sulfate and sodium keratan sulfate, and preferably sodium chondroitin sulfate.

As the component (A), one kind or two or more kinds of them are blended in the external preparation for skin of the present invention, and the blending amount is
Although not particularly limited, preferably 0.0001% by weight (hereinafter, simply referred to as “%”) or more, particularly 0.001 to 0.5%, relative to the total amount of the external preparation for skin of the present invention, excellent skin roughness and A wrinkle-improving effect is obtained, and storage stability and usability are excellent, which is preferable. If it is less than 0.0001%, it may be difficult to expect a sufficient effect, and if it exceeds 0.5%, it is uneconomical because the enhancement of the effect is hardly recognized. Regarding the method of addition, it may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

As the collagen extracted from fish as the component (B) used in the present invention, the fish skin, the floating bag and the like are washed, degreased, acid filtered, enzyme treated, salted out, dialyzed, heat treated, etc. Collagen obtained by extraction and purification, as the type of fish, salmon, cod, trout, flatfish, sea bream, flounder and the like can be mentioned as preferred, but it is not particularly limited and may be appropriately selected as needed. Good.

The collagen extracted from fish as the component (B) used in the present invention may be used as it is as an extracted solution. If necessary, it may be concentrated, diluted, filtered, hydrolyzed, or treated with pepsin. It may be used after processing such as conversion. Further, it may be used as a dried product after being subjected to treatments such as concentration dryness, spray drying and freeze drying.

The blending amount of the component (B) used in the present invention is 0.0001% or more, especially 0.001 to 0.8% in terms of solid content, based on the total amount of the external preparation for skin of the present invention. This is preferable because it provides an excellent effect of improving rough skin and wrinkles, and is excellent in storage stability and usability. If it is less than 0.0001%, it may be difficult to expect a sufficient effect.
When it is blended in excess of 8%, it is difficult to recognize the enhancement of the effect and it is uneconomical. Regarding the method of addition, it may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

The component (B) used in the present invention may be a commercially available one, for example, Neptigen atelotype (solid content 1%) sold by Ichimaru Falcos Co., Ltd. , Neptigen natural type (solid content 1%), Neptigen natural type (PF)
(Solid content 1%) and Alfomaline-CL (solid content 0.3%) sold by Technoble Co., Ltd., and acid-soluble atheroinated marine collagen solution (solid content sold by Ihara Suisan Co., Ltd.) 1%) and the like.

In the external preparation for skin of the present invention, if necessary, in addition to the above-mentioned essential components, various optional components usually used in cosmetics, pharmaceuticals, quasi-drugs, etc., within the range that does not impair the effects of the present invention. Can be appropriately mixed. Examples of such optional components include purified water, fats and oils, waxes,
Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, UV absorbers, thickeners, pigments, antioxidants,
Whitening agents, chelating agents and the like can be mentioned.

The external preparation for skin of the present invention comprises cosmetics, pharmaceuticals,
It can be used in any of quasi-drugs, and its dosage forms include, for example, lotion, cream, emulsion, gel, aerosol, ointment, poultice, essence, pack, detergent, bath agent. , Foundation, dusting powder, lipstick, etc. can be applied to the skin.

[0017]

EXAMPLES The present invention will now be described in detail with reference to examples, but the present invention is not limited thereto. The parts of the compounding amounts shown in the examples are parts by weight.

Example 1 Cream 1 Formulation Compounding amount 1. Neptigen Atelotype (Ichimaru Falcos Co., Ltd., solid content 1%) 5.0 parts 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Perfume 0.1 11. Chondroitin sulfate sodium 0.2 12.1, 3-butylene glycol 7.5 13. Ethyl paraoxybenzoate 0.05 14. Methyl paraoxybenzoate 0.2 15. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 9 are dissolved by heating and mixed, and the mixture is heated to 70 ° C.
Keep it in the oil phase. Ingredients 1 and 11 to 15 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. Add the water phase to the oil phase to emulsify, cool with stirring and mix at 10
Is added, and the product is further cooled to 30 ° C.

Example 2 Cream 2 Cream 2 was prepared by replacing the Neptigen atelotype in Example 1 with a Neptigen natural type (manufactured by Ichimaru Falcos Co., Ltd., solid content 1%).

Example 3 Cream 3 In Example 1, the neptigen atelotype was replaced with an acid-soluble atelo-ized marine collagen solution (Ihara Suisan Co., Ltd.,
The cream 3 was replaced with the solid content 1%).

Comparative Example 1 Conventional Cream 1 In Example 1, collagen extracted from cattle with Neptigen atelotype (Collagen Q manufactured by Sansho Pharmaceutical Co., Ltd.)
-A, solid content 1%) was replaced with the conventional cream 1 containing no chondroitin sulfate sodium.

Comparative Example 2 Conventional Cream 2 In Example 1, the conventional cream 2 was prepared by replacing the neptigen atelotype with purified water.

Example 4 Lotion 1 Formulation Amount 1. Acid-soluble atherogenic marine collagen solution (Ihara Suisan Co., Ltd., solid content 1%) 80.0 parts 2.1,3-butylene glycol 3.0 3. Glycerin 1.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.1 7. Chondroitin sodium sulfate 0.5 8. Ethanol 1.0 9. Methyl paraoxybenzoate 0.1 10. Polyoxyethylene hydrogenated castor oil (40 EO) 0.1 11. Perfume proper amount 12. [Production method] The total amount is 100 with purified water Components 1 to 7 and 12 and components 8 to 11 are uniformly dissolved, and both are mixed and filtered to obtain a product.

Example 5 Lotion 2 In Example 4, the compounding amount of the acid-soluble atheroinated marine collagen solution (Ihara Suisan Co., Ltd., solid content 1%) was 1
Toner lotion 2 was replaced with 0.0 part and the amount of sodium chondroitin sulfate added was replaced with 0.1 part.

Example 6 Lotion 3 In Example 4, the amount of the acid-soluble atherosclerotic marine collagen solution (Ihara Suisan Co., Ltd., solid content 1%) was replaced with 1.0 part, and the amount of sodium chondroitin sulfate was added. Toner lotion 3 was prepared by replacing 0.01 parts with 0.01 parts.

Example 7 Lotion 4 In Example 4, the amount of the acid-soluble atelolated marine collagen solution (Ihara Suisan Co., Ltd., solid content 1%) was replaced with 0.1 part, and the amount of sodium chondroitin sulfate was added. Was replaced with 0.001 part to give lotion 4.

Example 8 Emulsion formulation Compounding amount 1. Alphomarin-CL (Technoble Co., Ltd., solid content 0.3%) 80.0 parts 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetanol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene cetyl ether (20EO) 3.0 7. Polyoxyethylene sorbitan monooleate (20EO) 2.0 8. Perfume 0.1 9. Propylene glycol 1.0 10. Glycerin 2.0 11. Dermatan sodium sulfate 0.01 12. Methyl paraoxybenzoate 0.2 13. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 7 are dissolved by heating and mixed, and the temperature is 70 ° C.
Keep it in the oil phase. Ingredients 1 and 9 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, the mixture is cooled with stirring, component 8 is added at 45 ° C, and the mixture is further cooled to 30 ° C to obtain a product.

Example 9 Gel formulation Formulation amount 1. Neptigen Atelotype (Ichimaru Falcos Co., Ltd., solid content 1%) 40.0 parts 2. Ethanol 5.0 3. Methyl paraoxybenzoate 0.1 4. Polyoxyethylene hydrogenated castor oil (60 EO) 0.1 5. Fragrance suitable amount 6.1,3-butylene glycol 5.0 7. Glycerin 5.0 8. Xanthan gum 0.1 9. Carboxyvinyl polymer 0.2 10. Heparan sodium sulfate 0.015 11. Potassium hydroxide 0.2 12. [Production method] Components 2 to 5 and components 1 and 6 to 12 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed to obtain a product.

Example 10 Ointment Formulation Amount 1. Alphomarin-CL (Technoble Co., Ltd., solid content 0.3%) 1.5 parts 2. Polyoxyethylene cetyl ether (30EO) 2.0 3. Glycerin monostearate 10.0 4. Liquid paraffin 5.0 5. Cetanol 6.0 6. Methyl paraoxybenzoate 0.1 7. Propylene glycol 10.0 8. Keratan sodium sulfate 0.05 9. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 6 are dissolved by heating and mixed, and the temperature is 70 ° C.
Keep it in the oil phase. Ingredients 1 and 6 to 9 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring.

[0030]

Next, experimental examples will be given in order to explain the effects of the present invention in detail.

Experimental Example 1 Hyaluronidase Inhibitory Action Samples (1) to (4) below: (1) Evaporated dry matter of Neptigen natural type (manufactured by Ichimaru Falcos Co., Ltd., solid content 1%) and sodium chondroitin sulfate, etc. Amount mixture (2) Evaporated dry solid of acid-soluble atelolated marine collagen solution (manufactured by Ihara Suisan Co., Ltd., solid content 1%) and an equal amount mixture of sodium chondroitin sulfate (3) Alphomarin CL (manufactured by Technoble Co., Ltd.,
Evaporated dry matter of solid content 0.3%) and an equal amount mixture of sodium chondroitin sulfate (4) Evaporated dry matter of collagen QA (manufactured by Sansho Pharmaceutical Co., solid content 1%) and sodium chondroitin sulfate etc. The amount of hyaluronidase inhibitory effect of Morgan-
Method applying the Elson method [Food Hygiene Journal, 31,
3 (1990)]. That is, 0.1 mol / L acetate buffer (pH 4.0) 175 was added to the sample solution.
Add mL to increase the enzymatic activity of hyaluronidase to 400
U / mL, hyaluronic acid concentration 0.4 mg / mL, activator compound 48/80 0.06 mg / mL
Then, the total amount was adjusted to 500 mL so that the hyaluronidase reaction was carried out at 37 ° C. for 40 minutes. After the reaction, a p-dimethylaminobenzaldehyde reagent was added to develop color, and the absorbance at 585 nm was measured. The inhibitory action of each sample was calculated by the inhibition rate calculated from the following formula. As a control, purified water was used instead of the sample, and purified water was used instead of hyaluronidase as a blank. Inhibition rate (%) = (1− (C−D) / (A−B)) × 10
0 A: Absorbance of control at 585 nm (OD 58)
5) B: O.V. D. 585 C: O.D. D. 585 D: O. D. 585

The results of these experiments are shown in Table 1. As a result, it was shown that Samples (1) to (3) had a stronger hyaluronidase inhibitory action than Sample (4).

[0033]

[Table 1]

Experimental Example 2 Usage Test Using Cream 1 of Example 1, Cream 2 of Example 2, Cream 3 of Example 3, Conventional Cream 1 of Comparative Example 1 and Conventional Cream 2 of Comparative Example 2, Woman worried about fine lines 35
A one-month use test was conducted on humans (21 to 48 years old). After use, the effect of improving fine wrinkles was evaluated by a questionnaire. The results are shown in Table 2. As a result, cream 1
The external preparations for skin of Nos. 3 to 3 showed an excellent effect of improving fine wrinkles.
During the test period, there was no skin trouble and there was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0035]

[Table 2]

Using the emulsion of Example 8, the gel of Example 9 and the ointment of Example 10 in the same manner as above, the use of the emulsion was found to be excellent.

Experimental Example 3 Usage Test Using lotion 1 of Example 4, lotion 2 of Example 5, lotion 3 of Example 6, lotion 4 of Example 7, 30 women suffering from fine wrinkles ( A use test for one month was conducted for 38 to 59 years old). After use, the effect of improving fine wrinkles was evaluated by a questionnaire. The results are shown in Table 3. As a result, a lotion containing a large amount of the acid-soluble atelolated marine collagen solution (made by Ihara Suisan Co., Ltd., solid content: 1%) and sodium chondroitin sulfate showed an excellent effect of improving fine wrinkles. There was no skin trouble during the test period,
There was no problem in safety. In addition, there was no problem with the deterioration of the prescription ingredients.

[0038]

[Table 3]

As shown above, the external preparation for skin of the present invention exhibited excellent effects of improving rough skin and wrinkles.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 31/737 A61K 31/737 38/17 A61P 17/16 A61P 17/16 A61K 37/12 (72) Invention Miki Aoki 2-7 Torimicho, Nishi-ku, Nagoya-shi Japan Menard Cosmetics Co., Ltd. Research Institute (72) Inventor Izumi Izumi 2-7 Torimicho, Nishi-ku, Nagoya-shi Japan Menard Cosmetics Co., Ltd. Research Institute (72) Inventor Satoru Nakata Nagoya 2-7 Torimicho, Nishi-ku, Japan F-Term (Reference) at Research Institute of Japan Menard Cosmetics Co., Ltd. 4C083 AA082 AA122 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC342 AC422 AC432 AC442 AC482 AD092 AD311 AD321 AD341 AD342 AD352 AD431 AD432 CC02 CC04 CC05 DD27 DD33 DD41 EE12 4C084 AA03 BA44 CA45 DA40 MA02 MA17 MA28 MA63 ZA891 ZA892 4C086 AA01 AA02 EA23 EA2 6 MA02 MA04 MA63 ZA89

Claims (9)

[Claims] 1. The following components (A) and (B): (A) Mucopolysaccharide (B) Collagen extracted from fish An external preparation for skin, which comprises: 2. The external preparation for skin according to claim 1, wherein
A skin external preparation characterized in that collagen extracted from fish as component (B) is water-soluble. 3. The external preparation for skin according to claim 1, wherein collagen extracted from the fish of component (B) is atherolated by pepsin treatment. 4. The external skin preparation according to any one of claims 1 to 3, wherein the collagen extracted from the component (B) fish is one selected from salmon, cod, trout, flounder, quince, and flounder, or A skin external preparation characterized by being collagen extracted from two or more kinds. 5. The external preparation for skin according to any one of claims 1 to 4, wherein the content of collagen extracted from the component (B) fish is 0 in terms of solid content based on the total amount of the external preparation for skin. 0.001 to 0.8% by weight of an external preparation for skin. 6. The external preparation for skin according to claim 1, wherein
A skin external preparation characterized in that the component (A) is one or more components selected from chondroitin sulfate, dermatan sulfate, heparan sulfate, keratan sulfate and chitin, and salts thereof. 7. The external skin preparation according to claim 1, wherein the component (A) is chondroitin sulfate and / or its salt. 8. The external preparation for skin according to claim 1, wherein the compounding amount of the component (A) is from 0.001 to 0.001.
A skin external preparation characterized by being 0.5% by weight. 9. The external preparation for skin according to claim 1, wherein the dosage form is a cosmetic, a drug, or a quasi drug.