KR20230020834A - An antibacterial composition comprising champsocephalus gunnari extract or notothenia rossii extract - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising an antarctic meckel smelt (Champsocephalus gunnari) extract or antarctic marbled blackfish (Notothenia rossii) extract as an active ingredient. Since the antarctic meckel smelt (Champsocephalus gunnari) extract or antarctic marbled blackfish (Notothenia rossii) extract of the present invention has excellent antibacterial activity against Staphylococcus aureus, a composition containing it as an active ingredient has an antibacterial activity and can be usefully used in as pharmaceuticals (external skin preparations), quasi-drugs, food, all-purpose cleanser (skin cleanser, hair cleanser, vaginal wash, mouthwash, bathroom cleaner, kitchen detergent, etc.), preservatives (cosmetic preservatives, food preservatives, pharmaceutical preservatives), antiseptic, additive (food additive, cosmetic additive, feed additive) material as well as cosmetics. In particular, the Champsocephalus gunnari or Notothenia rossii is edible as natural substances, so the antibacterial composition of the present invention containing an extract derived therefrom has the advantage of remaining safe even for long-term use.

Description

An antibacterial composition comprising champsocephalus gunnari extract or notothenia rossii extract}

The present invention relates to an antibacterial composition comprising an extract of Antarctic Meckel's smelt ( Champsocephalus gunnari ) or an extract of Antarctic marbled female fish ( Notothenia rossii ) as an active ingredient.

Microorganisms exist all around us. In particular, telephones, mobile phones, flooring materials, refrigerators, air conditioners, humidifiers, toys, wallpaper, and various gas kits that we can easily access in our daily lives are prone to propagation of various germs and molds, causing odors, and even cause human body odor. Harmful bacteria proliferate and cause various diseases, and serious social problems such as food poisoning by pathogenic Escherichia coli O-157 and legionella bacteria that cause pneumonia in public baths occur. Therefore, a highly functional antibacterial material is required to prevent this, and many studies on these antibacterial materials have been conducted to date.

Direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental, and medical problems. There are many problems throughout society, such as losses due to spoilage during food distribution in the food industry, harm to the human body and environmental pollution due to the use of excessive chemical pesticides on crops in the agricultural industry, and the emergence of antibiotic-resistant strains due to misuse and abuse of antibiotics. are causing them

In addition, the awareness of the importance of the microbial contamination problem of cosmetics is increasing due to the hygienic aspect of the skin area as well as the deterioration of the product value due to the deterioration of the product itself. Cosmetics use a lot of nutrients derived from natural products and raw materials that are easily decomposed by microorganisms, so they are easily contaminated by microorganisms such as mold, yeast, and bacteria during distribution or use by consumers. In order to suppress, preservatives are added, and the minimum amount should be used to show the maximum antibacterial activity. Therefore, it is necessary to consider the combination of nutrients in cosmetic products, pH, emulsified form, mixed use of preservatives, and their stability, as well as antibacterial activity and skin irritation of all raw ingredients in the product beforehand to determine each product Selecting an appropriate preservative system for each type is very important. And because of the various side effects of synthetic preservatives, the development of natural preservatives has emerged as a major concern in the cosmetic industry.

On the other hand, Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ) are fish that live only in Antarctica as marine living resources.

Therefore, the inventors of the present invention are natural materials having excellent antibacterial properties against various pathogens, and to find environmentally friendly materials that can be applied to cosmetics as well as pharmaceuticals, quasi-drugs, foods, detergents, cleaners, preservatives, preservatives, food additives and feed additives, etc. During the search, attention was paid to Antarctic fish and their pharmacological effects were tested. As a result, in the case of extracts from Antarctic Mekkel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ), Staphylococcus aureus, a Gram-positive facultative anaerobic bacterium, was found. The present invention was completed by confirming that the antimicrobial activity against cocci ( Staphylococcus aureus ) was excellent.

Korean Patent Registration No. 10-2141495

Accordingly, an object of the present invention is to provide a composition that is safe to the human body and has excellent antibacterial activity even for long-term use by using a natural material extract derived from Antarctic fish.

Another object of the present invention is to provide a cosmetic composition that is safe to the human body and has excellent antibacterial activity even for long-term use by using a natural material extract derived from Antarctic fish.

Another object of the present invention is to provide a composition for external application for skin using a natural material extract derived from Antarctic fish that is safe for the human body and has excellent antibacterial activity even after long-term use.

Another object of the present invention is to provide a food composition that is safe to the human body and has excellent antibacterial activity even for long-term use by using a natural material extract derived from Antarctic fish.

Another object of the present invention is to provide a detergent composition that is safe to the human body and has excellent antibacterial activity even for long-term use by using a natural material extract derived from Antarctic fish.

In order to achieve the object of the present invention as described above,

The present invention provides an antibacterial composition comprising an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled female fish ( Notothenia rossii ) extract as an active ingredient.

In one embodiment of the present invention, the extract may be extracted from a portion selected from the group consisting of skin, brain, liver, stomach, and muscle of Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ).

In one embodiment of the present invention, the extract may be extracted with one or more solvents selected from the group consisting of lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, water and hexane.

In one embodiment of the present invention, the extract may have antibacterial activity against Gram-positive bacteria.

In one embodiment of the present invention, the Gram-positive bacteria may be Staphylococcus aureus .

In one embodiment of the present invention, the antibacterial composition may be used in products selected from the group consisting of cosmetics, pharmaceuticals, quasi-drugs, foods, detergents, cleaners, preservatives, preservatives, food additives and feed additives.

In addition, the present invention provides an antibacterial cosmetic composition comprising an extract of Antarctic Meckel smelt ( Champsocephalus gunnari ) or an extract of Antarctic marbled female tooth ( Notothenia rossii ) as an active ingredient.

In addition, the present invention provides an antibacterial composition for external application for skin comprising an extract of Antarctic Meckel smelt ( Champsocephalus gunnari ) or an extract of Antarctic marble-patterned female tooth ( Notothenia rossii ) as an active ingredient.

In addition, the present invention provides an antibacterial food composition comprising an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled female fish ( Notothenia rossii ) extract as an active ingredient.

In addition, the present invention provides an antibacterial detergent composition comprising an Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or an Antarctic marbled female tooth ( Notothenia rossii ) extract as an active ingredient.

Since the Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or the Antarctic marbled female tooth ( Notothenia rossii ) extract of the present invention has excellent antibacterial activity against Staphylococcus aureus , a composition containing it as an active ingredient is a composition having antibacterial activity. In addition to cosmetics, pharmaceuticals (external skin products), quasi-drugs, foods, multi-purpose cleaners (skin cleanser, hair cleanser, vaginal cleanser, mouthwash, bathroom cleanser, kitchen cleanser, etc.), preservatives (cosmetic preservatives, food preservatives, pharmaceutical preservatives) , preservatives, additives (food additives, cosmetic additives, feed additives) can be usefully used. In particular, since Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ) are edible as natural substances, the antibacterial composition of the present invention containing an extract derived from them has the advantage of being safe even for long-term use.

1a to 1c are photographs visually confirming the growth degree of Staphylococcus aureus after each concentration of the Antarctic fish extract (S1 to S10) of the present invention was treated in a solid culture medium.
2a to 2c are photographs visually confirming the growth degree of Escherichia coli after each concentration of the Antarctic fish extract (S1 to S10) of the present invention was treated in a solid culture medium.
3a to 3d are graphs showing the growth inhibitory activity against Staphylococcus aureus according to treatment at each concentration of the Antarctic fish extract (S1, S3, S4, and S9) of the present invention (3a: S1 , 3b: S3, 3c: S4, 3d: S9).
4a to 4c are photographs visually confirming the growth degree of Candida albicans after treating each of the Antarctic fish extracts (S1 to S10) of the present invention by concentration in a solid culture medium.

The present invention is characterized by providing an antibacterial composition comprising an extract of Antarctic Meckel's smelt ( Champsocephalus gunnari ) or an extract of Antarctic marble-patterned female tooth ( Notothenia rossii ) as an active ingredient.

The "Antarctic Meckel smelt extract or Antarctic marbled smelt extract" of the present invention is directly extracted and separated from Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled smelt ( Notothenia rossii ) using an extraction and separation method known in the art. The 'extract' defined in the present invention can be obtained by extracting the skin, brain, liver, stomach, and muscle of the Antarctic fish using an appropriate solvent. etc., and includes, for example, crude extracts from various parts of the Antarctic fish (skin, brain, liver, stomach, muscle), polar solvent-soluble extracts, or non-polar solvent-soluble extracts.

Any pharmaceutically acceptable organic solvent may be used as a suitable solvent for extracting the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention, and water or an organic solvent may be used, but is not limited thereto. , For example, alcohol having 1 to 4 carbon atoms including purified water (water), methanol, ethanol, propanol, isopropanol, butanol, etc., acetone, Various solvents such as ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane can be used alone or in combination. can Preferably, it is good to use hexane.

As an extraction method, any one of methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the preparation method of the extract of the present invention, and any known method may be used.

For example, the Antarctic Meckel smelt extract or Antarctic marbled female fish extract of the present invention is obtained by converting the primary extract extracted by the hot water extraction or solvent extraction method into a powder state by an additional process such as vacuum distillation and freeze drying or spray drying. can be manufactured In addition, a fraction further purified from the primary extract using various chromatography methods such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography you can also get

Therefore, in the present invention, the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract is a concept that includes all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and dilutions, concentrates, or dried products thereof.

In one embodiment of the present invention, the extract is obtained by freezing and drying the skin, brain, liver, stomach and muscle tissue of Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female fish ( Notothenia rossii ), respectively, and then pulverizing and adding hexane to can be extracted.

The present inventors have demonstrated through experiments that the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract has an antibacterial effect against Gram-positive bacteria, Staphylococcus aureus (see Experimental Example 1).

Therefore, the composition of the present invention containing the Antarctic Meckel's smelt extract or the Antarctic marble-patterned female chum extract as an active ingredient can be usefully used as an antibacterial material.

In the present invention, 'antibacterial composition' may mean the same as antibiotics, which is a generic term for antimicrobial agents, and may mean the same as antibacterial agents, bactericides, preservatives, preservatives, or disinfectants, and may cause food poisoning or purulent It refers to a substance capable of inhibiting or suppressing the growth and life function of pathogenic microorganisms such as Staphylococcus aureus , which is the cause of infection.

' Staphylococcus aureus ' is a gram-positive facultative anaerobic bacterium, which is a pathogen commonly present on the skin and nasal surfaces of healthy people or livestock, and causes food poisoning by producing heat-resistant exotoxins. It is also known to secrete phagocyte-killing toxins (leucocidin), hemolysin, coagulation enzymes, etc., to break free from the resistance of infected host cells and cause pyogenic infections. Staphylococcus aureus is commonly found on the skin and nasal passages of more than 50% of healthy people and animals, and can cause a variety of diseases ranging from mild skin infections such as acne and impetigo to life-threatening diseases such as pneumonia, meningitis, and sepsis. . It is also one of the five most common causes of nosocomial infections, often leading to postoperative wound infections. About 500,000 patients in US hospitals are known to contract staph infections each year. Staphylococcus aureus can secrete various toxins depending on the strain, which are classified into three types: pyrogenic toxin superantigens, exfoliative toxins, and other toxins. The strains that produce the 7 enterotoxins that cause food poisoning belong to superantigens of febrile toxins. Enterotoxin produced by Staphylococcus aureus causes gastroenteritis, which causes symptoms such as nausea, vomiting, diarrhea, and abdominal pain, and the symptoms are relieved and healed naturally within 8-24 hours. Food poisoning symptoms are caused by enterotoxins, not by the bacteria themselves, and because they are heat resistant, they are not destroyed even when heated or cooked, so it is best not to consume foods contaminated with Staphylococcus aureus.

The antibacterial composition of the present invention can be used for various purposes and uses requiring antibacterial activity, and specifically, it can be used as cosmetics, pharmaceuticals, quasi-drugs, foods, detergents, cleaners, preservatives, preservatives, food additives and feed additives.

First, the composition of the present invention may be an antibacterial cosmetic composition.

The antibacterial cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, as well as Antarctic Meckel smelt extract or Antarctic marbled female fish extract. It may include conventional adjuvants such as, and carriers.

In addition, the antibacterial cosmetic composition of the present invention, in addition to the above-mentioned active ingredients (Antarctic Meckel smelt extract or Antarctic marble-patterned female fish extract), reacts with active ingredients to the extent that the skin protection effect is not impaired. Mixing may also be used.

Products to which the antibacterial cosmetic composition of the present invention can be added include, for example, astringent lotion, softening lotion, nutrient lotion, cosmetics such as various creams, essences, packs, foundations, and the like, as well as cleansing, facial cleansers, soaps, and treatments. , serum, etc.

Specific formulations of the antibacterial cosmetic composition of the present invention include flexible lotion, gel, water-soluble liquid, milk lotion, nourishing cream, massage cream, essence, oil-in-water emulsion, water-in-oil emulsion, facial anhydrous product, solid anhydrous product, small Oil dispersion in aqueous phase using spheres, ionic lipid vesicles, nonionic lipid vesicles, ointments, cleansing foams, cleansing water, packs, body oils, oil-in-water makeup bases, water-in-oil makeup bases, foundations, skin covers, It includes one formulation selected from the group consisting of lipstick, lip gloss, face powder, two-way cake, eye shadow, mascara, cheek color, and eyebrow pencil.

According to a preferred embodiment of the present invention, the content of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled female fish extract) of the present invention is 0.0001-20% by weight, preferably 0.5-20%, based on the total weight of the composition, More preferably, it is 1.0-20 weight%. If the content of the active ingredient (Antarctic Meckel smelt extract or Antarctic marble-patterned female fish extract) is less than 0.0001% by weight, the antibacterial effect is greatly reduced, and if it exceeds 20% by weight, skin irritation may be caused, and problems in formulation can happen

On the other hand, the antibacterial cosmetic composition according to the present invention may be formulated by stabilizing the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled female fish extract) by containing it inside the nanoliposome. When the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled smelt extract) is contained inside the nanoliposome, the components of the active ingredient (Antarctic Meckel smelt extract or Antarctic marbled smelt extract) are stabilized, resulting in formation of sediment, discoloration, Problems such as off-odor can be solved, and the solubility and transdermal absorption rate of components can be increased, so that the efficacy expected from the extract can be maximized.

In the present invention, the nanoliposome has the form of a conventional liposome, and means a liposome having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposome is 50 to 300 nm. When the average particle diameter of the nanoliposome exceeds 300 nm, improvement of skin penetration and improvement of formulation stability among the technical effects to be achieved in the present invention are very weak.

According to the present invention, the nanoliposome used to stabilize the extract may be prepared by a mixture containing a polyol, an oily component, a surfactant, a phospholipid, a fatty acid, and water.

Polyols used in the nanoliposomes of the present invention are not particularly limited, and are preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isopropylene glycol, pentylene glycol, erythritol, and xylitol. , sorbitol, and at least one selected from the group consisting of mixtures thereof. The amount used is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of the nanoliposome.

Various oils known in the art may be used as the oil component used in the preparation of the nanoliposomes of the present invention, preferably hydrocarbon-based oils such as hexadecane and paraffin oil, ester-based synthetic oils, Silicone oils such as dimethicone and cyclomethicone, animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils , phytosphingosine, sphingonoid lipids such as sphingosine and sphinganine, cerebroside cholesterol, sitosterol cholesteryl sulfate, sitosteryl sulfate, C10-40 fatty alcohols and mixtures thereof. The amount used may be 1.0 to 30.0% by weight, preferably 3.0 to 20.0% by weight, based on the total weight of the nanoliposome.

Any surfactant known in the art may be used as the surfactant used in the preparation of the nanoliposome of the present invention. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkylacylglutamates, alkylphosphates, alkylactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount used may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.

Phospholipids, which are another component used in the preparation of the nanoliposomes of the present invention, are amphiphilic lipids, natural phospholipids (e.g., egg yolk lecithin or soybean lecithin, sphingomyelin) and synthetic phospholipids (e.g., dipalmitoylphosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated or saturated lecithin of natural origin extracted from soybean or egg yolk is preferred. Typically, naturally-derived lecithin has an amount of phosphatidylcholine of 23 to 95% and an amount of phosphatidylethanolamine of 20% or less. In the preparation of the nanoliposome of the present invention, the amount of phospholipid used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight, based on the total weight of the nanoliposome.

The fatty acids used in the preparation of nanoliposomes of the present invention are higher fatty acids, preferably C12-22 alkyl chain saturated or unsaturated fatty acids, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. include The amount used may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of the nanoliposome.

Water used in the preparation of the nanoliposome of the present invention is generally deionized distilled water, and the amount used may be 5.0 to 40% by weight based on the total weight of the nanoliposome.

Nanoliposomes may be prepared through various methods known in the art, but most preferably, they are prepared by applying a mixture containing the above components to a high-pressure homogenizer. The preparation of nanoliposomes by a high-pressure homogenizer can be carried out under various conditions (e.g., pressure, number of times, etc.) depending on the desired particle size, preferably 1 to 5 times under a pressure of 600 to 1200 bar. Nanoliposomes can be prepared by passing through.

The cosmetic composition for improving skin wrinkles according to the present invention may contain 0.1 to 20.0% by weight of the active ingredient (Antarctic Merkel smelt extract or Antarctic marble-patterned female fish extract) based on the total weight of nanoliposomes.

In addition, the composition of the present invention may be an antibacterial pharmaceutical composition.

The pharmaceutical composition of the present invention can be prepared using pharmaceutically suitable and physiologically acceptable adjuvants in addition to the above active ingredients (Antarctic Meckel smelt extract or Antarctic marble-patterned amphibian extract), and the adjuvants include excipients and disintegrants , sweeteners, binders, coating agents, expanding agents, lubricants, lubricants, or flavoring agents may be used.

For administration, the pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients (Antarctic Meckel smelt extract or Antarctic marbled smelt extract). .

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops, or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like. In compositions formulated as liquid solutions, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added if necessary. In addition, a diluent, a dispersing agent, a surfactant, a binder, and a lubricant may be additionally added to formulate an injection formulation such as an aqueous solution, suspension, or emulsion, pill, capsule, granule, or tablet. Furthermore, using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field, it can be preferably formulated according to each disease or component.

In one embodiment of the present invention, the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention may be included in an amount of 0.0001 to 50% by weight based on the total weight of the composition.

In another embodiment of the present invention, the pharmaceutical composition of the present invention may be an external composition for skin.

The external skin pharmaceutical composition of the present invention is a cream , gel, patch, spray, ointment, warning agent, lotion, liniment agent, pasta agent, or It can be prepared and used as a pharmaceutical composition in the form of a skin external preparation of cataplasma agent, but is not limited thereto.

In addition, the composition of the present invention may be an antibacterial food composition.

The antibacterial food composition may contain various flavoring agents or natural carbohydrates as additional ingredients, as in conventional food compositions, in addition to containing the active ingredient, Antarctic Meckel smelt extract or Antarctic marbled female fish extract.

Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agents described above, natural flavoring agents (thaumatin), stevia extracts (eg rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can advantageously be used.

The antibacterial food composition of the present invention is formulated in the same way as the pharmaceutical composition and used as a functional food, or may be added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverages, vitamin complexes and health supplements, etc. there is

In addition, the antibacterial food composition is an active ingredient (Antarctic Meckel smelt extract or Antarctic marbled female fish extract) in addition to various nutrients, vitamins, minerals (electrolyte), flavors such as synthetic flavors and natural flavors, colorants and enhancers ( cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages.

The active ingredient of the present invention (Antarctic Meckel smelt extract or Antarctic marbled mackerel extract) is a natural material extracted from edible Antarctic fish and has little side effects such as chemicals, so Gram-positive bacteria are disease related to Staphylococcus aureus For the purpose of providing improved functionality, it can be safely used even when taking for a long time.

In addition, when the antibacterial composition of the present invention is used as a detergent material, the article to which the antimicrobial composition can be applied is not particularly limited, specifically hand wash, body wash, shampoo, rinse, cleansing foam, cleansing gel , Soap, gargle, toothpaste, bathroom cleaner, kitchen cleaner and the like can be exemplified. The Antarctic Meckel smelt extract or Antarctic marbled female fish extract of the present invention may be included in an amount of 0.00001 to 50.0% by weight based on the total weight of the detergent composition, and may be included in the composition at a concentration of 1 μg / ml to 1000 μg / ml. In addition, the cleaning agent may further contain other known antibacterial substances having antibacterial activity, in addition to the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention.

In addition, when the antibacterial composition of the present invention is used as a preservative or preservative material, it is not particularly limited in the article to which the antimicrobial composition can be applied, specifically cosmetics, food, pharmaceuticals and the like can be exemplified. The Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention may be included in an amount of 0.00001 to 50.0% by weight based on the total weight of the preservative or preservative composition, and may be included in the composition at a concentration of 1 μg / ml to 1000 μg / ml. In addition, the preservative or preservative may further include other known antibacterial substances having antibacterial activity or antiseptic activity, in addition to the Antarctic Meckel smelt extract or the Antarctic marble-patterned female fish extract of the present invention.

In addition, when the antibacterial composition of the present invention is used as an additive material, the article to which the antimicrobial composition can be applied is not particularly limited, and specifically, food and animal feed can be exemplified. The Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention may be included in an amount of 0.00001 to 50.0% by weight based on the total weight of the additive composition, and may be included in the composition at a concentration of 1 μg / ml to 1000 μg / ml. In addition, the food additive or feed additive may further include other known antibacterial substances having antibacterial activity or substances capable of imparting other functions, in addition to the Antarctic Meckel smelt extract or the Antarctic marbled female fish extract of the present invention.

Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited to these examples.

<Example 1>

Preparation of Antarctic fish extract of the present invention

Frozen Antarctic fishes, Notothenia rossii (hereinafter simply abbreviated as 'NR') and Champsocephalus gunnari (hereinafter simply abbreviated as 'CG') were each thawed. In the case of NR, the samples were directly collected from the coast of King Sejong Station in Antarctica, and in the case of CG, bycaught fish were delivered from a deep-sea fishing company that is engaged in krill fishing. Each individual was dissected to obtain tissue samples (skin, brain, liver, stomach, muscle), washed with distilled water, and freeze-dried. From the dried tissue samples, NR: skin 13.20 g, brain 0.35 g, liver 2.74 g, stomach 3.12 g, CG: skin 8.50 g, brain 0.22 g, liver 8.36 g, stomach 2.78 g, muscle 1.78 g were obtained. After each sample was crushed using a mortar and pestle, each was transferred to a 200 mL glass bottle, and then 200 mL of 95% hexane was added and extracted twice a day at room temperature (24 hr X 2). Each of the extracts obtained through the above process was transferred to a round flask and the solvent was completely removed using a vacuum concentrator. As a concentrated extract from which the solvent was removed, NR: skin 0.18 g, brain 0.13 g, liver 0.24 g, stomach 0.21 g, CG: skin 0.41 g, brain 0.08 g, liver 1.78 g, stomach 0.52 g, muscle 1.82 g were finally obtained. was obtained, and the experiment was conducted using this.

Antarctic fish extract of the present invention extract sample extraction solvent Pisces group S1 CG skin hexane S2 brain hexane S3 liver hexane S4 stomach hexane S5 muscle hexane S6 NR skin hexane S7 brain hexane S8 liver hexane S9 stomach hexane S10 muscle hexane

<Experimental Example 1>

Antibacterial and antifungal effect of the Antarctic fish extract of the present invention

In this experiment, in order to examine the antibacterial and antifungal function of the Antarctic fish extract of the present invention prepared in <Example 1>, Staphylococcus aureus , a gram-positive bacterium, and Escherichia coli , a gram-negative bacterium, were tested. Antibacterial activity and antifungal activity against Candida albicans were confirmed.

<1-1> Antibacterial activity

First, in order to confirm the antibacterial activity, as a qualitative analysis, the inhibition of microbial growth was determined by dropping the Antarctic fish extract of the present invention on a solid culture medium. In detail, 10 ⁸ cells of the two bacterial species were applied to LB solid medium, and then 10 ul of each of the Antarctic fish extracts of the present invention prepared in <Example 1> were treated for each concentration. The experiment was repeated twice for the same concentration. The concentration of the treated extract was 0.5, 1, 2, and 5 ug/ul, so that the amount of the extract finally used was 5, 10, 20, and 50 ug. The extract was dissolved in 50% DMSO, and 0.1 and 0.2 ug/ul of Kanamycin were used as positive controls. Each solid medium was incubated at 37 ° C. for 16 to 24 hours, and the degree of growth of the applied microorganisms was visually confirmed.

As a result, as shown in FIGS. 1a to 1c, it was confirmed that the Antarctic fish extracts S1, S3, S4 and S9 of the present invention had antibacterial activity against Staphylococcus aureus , a Gram-positive bacterium. On the other hand, no antibacterial activity was observed against Gram-negative Escherichia coli ( Escherichia coli ) (see FIGS. 2a to 2c).

In addition, in order to quantitatively determine the antibacterial activity of the four Antarctic fish extracts (S1, S3, S4, and S9) of the present invention, which showed growth inhibitory activity against Staphylococcus aureus, which is a Gram-positive bacterium, liquid culture of bacteria IC ₅₀ values were calculated. In detail, after diluting the Staphylococcus aureus culture medium with 0.5 McFarland Standard, 95ul each was dispensed into a 96-well plate, and each extract was treated by concentration (10, 20, 50, 100, 200, 500 ug/ml). After culturing at 37 ° C for 24 hours, the OD ₆₀₀ absorbance was measured with a plate reader to obtain the concentration when the cell decreased by 50%.

As a result, it was confirmed that S9, the Antarctic fish extract of the present invention, showed antibacterial activity depending on the treatment concentration (see FIG. 3d), and the IC ₅₀ value was measured as 188.16±26.63 ug/ml, confirming that it can be sufficiently used as an antibiotic. (see Table 2). On the other hand, the cell growth of the Antarctic fish extracts S1, S3 and S4 of the present invention was not reduced by 50% even at the highest treatment concentration of 500 ug/ml.

IC ₅₀ values for Staphylococcus aureus of Antarctic fish extracts (S1, S3, S4, S9) of the present invention Antarctic Fish Extract IC ₅₀ value S1 >500 ^* S3 >500 S4 >500 S9 188.16±26.63 Positive control (Kanamycin) 4.60±1.09

>500 ^* : Cell growth did not decrease by 50% even at the highest treatment concentration of 500 ug/ml

<1-2> Antifungal activity

In order to confirm the antifungal activity, as a qualitative analysis, the inhibition of microbial growth was determined by dropping the Antarctic fish extract of the present invention on a solid culture medium. Specifically, for the fungus Candida albicans, 10 ⁸ cells were applied to a GPY solid medium, and then 10 ul of each of the Antarctic fish extracts of the present invention was treated for each concentration. As in the bacterial experiment, the extract dissolved in 50% DMSO was treated at 0.5, 1, 2, and 5 ug/ul by 10 ul. As a positive control group, 0.05 ug/ul of Nystatin was used.

As a result, as shown in FIGS. 4a to 4c, it was confirmed that the Antarctic fish extracts (S1 to S10) of the present invention did not exhibit antifungal activity against Candida albicans .

Formulation Example 1: Soft lotion (skin lotion)

As shown in the table below, softening lotion was prepared according to a conventional method.

Formulation example of softening lotion of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 1,3-butylene glycol 6.0 glycerin 4.0 DL-panthenol 0.3 allantoin 0.1 polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 fragrance, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrition lotion (milk lotion)

As shown in the table below, nutrient lotion was prepared according to a conventional method.

Nutritional longevity formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitastearate 0.5 hydrogenated vegetable oil 4.0 mineral oil 5.0 trioctanoin 2.0 dimethicone 0.8 Tocopherol Acetate 0.5 carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 sodium hyaluronate 5.0 triethanolamine 0.12 Uniside-U 13 0.02 incense a very small amount Distilled water to 100

Formulation Example 3: Nutrition Cream

As shown in the table below, a nutritious cream was prepared according to a conventional method.

Nutritional cream formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 Pro-type glycerin monostearate 1.5 Cetearyl Alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitastearate 0.6 isostearyl isosterate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 Uniside-U13 0.02 incense a very small amount Distilled water to 100

Formulation Example 4: Massage Cream

As shown in the table below, a massage cream was prepared according to a conventional method.

Massage cream formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 propylene glycol 6 glycerin 4.0 triethanolamine 0.5 beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan Sesquioleate 2.5 Cetearyl Alcohol 2.0 liquid paraffin 30.0 carboxyvinyl polymer 0.5 incense a very small amount Distilled water to 100

Formulation Example 5: Pack

As shown in the table below, packs were prepared according to conventional methods.

Pack formulation example of the present invention Ingredients Content (% by weight) Antarctic fish extract of the present invention 1 to 5 glycerin 10.0 betaine 5.0 REG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 80. carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 Uniside-U13 0.01 incense a very small amount Distilled water to 100

So far, the present invention has been looked at with respect to its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (10)

Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or Antarctic marbled female fish ( Notothenia rossii ) An antibacterial composition comprising an extract as an active ingredient. According to claim 1,
The extract is an antibacterial composition, characterized in that extracted from a portion selected from the group consisting of skin, brain, liver, stomach and muscle of Antarctic Meckel smelt ( Champsocephalus gunnari ) or Antarctic marbled female tooth ( Notothenia rossii ). According to claim 1,
The extract is an antibacterial composition, characterized in that extracted with one or more solvents selected from the group consisting of lower alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, water and hexane. According to claim 1,
The extract is an antibacterial composition, characterized in that it has an antibacterial activity against Gram-positive bacteria. According to claim 4,
The Gram-positive bacteria is Staphylococcus aureus ( Staphylococcus aureus ) Antimicrobial composition, characterized in that. According to any one of claims 1 to 5,
The antimicrobial composition is an antimicrobial composition, characterized in that used for products selected from the group consisting of cosmetics, pharmaceuticals, quasi-drugs, food, detergents, cleaners, preservatives, preservatives, food additives and feed additives. Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or Antarctic marbled female fish ( Notothenia rossii ) An antibacterial cosmetic composition comprising an extract as an active ingredient. An antibacterial composition for external application for skin comprising an extract of Antarctic Merkel smelt ( Champsocephalus gunnari ) or an extract of Antarctic marbled female fish ( Notothenia rossii ) as an active ingredient. Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or Antarctic marbled female fish ( Notothenia rossii ) An antibacterial food composition comprising an extract as an active ingredient. Antarctic Meckel smelt ( Champsocephalus gunnari ) extract or Antarctic marbled female fish ( Notothenia rossii ) An antibacterial detergent composition comprising an extract as an active ingredient.

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