KR101989681B1 - Anti acne cosmetic composition and preparation method of the same comprising the mixed extract of Sophorolipid, Withania Somnifera Root, Coriandrum Sativum and Propolis - Google Patents

Abstract

The present invention relates to a soporolipid, a winter cherry root, a coriander and a propolis mixed extract of the present invention which inhibits the growth of acne bacterium. More particularly, the present invention relates to the growth of an acne bacterium, Propionibacterium acnes , The present invention provides an excellent cosmetic composition having excellent anti-acne functional effect and a method for producing the same.

Description

The present invention relates to an anti-acne functional cosmetic composition comprising as an active ingredient a sophorolipid, a winter cherry root, a mixture of coriander and propolis, and a method for preparing the same. , Coriandrum Sativum and Propolis}

The present invention relates to a sophorolipid, a winter cherry root, a mixture of coriander and propolis, and a method for producing the same, and more particularly, to a method for inhibiting the growth of an acne bacterium, Propionibacterium acnes , Which comprises an antioxidant active ingredient, which comprises as an active ingredient an extract of Sophorolipid, Winter cherry root, coriander and propolis.

Sophorolipid is a sophorolipid which is a 100% natural origin of glycolipid produced by the fermentation of lipidic of wheat glucidic and rapeseed oil with Candida bombicola. It is composed of Propionibacterium acnes , an acne bacterium, It is known that inhibition effect on Corynebacterium Xerosis, which is one of them, and inhibition of sebum production, are inhibited at 0.05% and 0.2% concentration, respectively. In addition, according to recent research results, when a gel containing 2% of sophorolipid was applied to a panel having 22 oily skin twice daily for 28 days, the amount of sebum was reduced by 12% and the rate of sebum production was also significantly reduced But also a significant reduction in pustules and pus.

Withania Somnifera Root is a small shrub that grows throughout India and the Middle East. It is the best Indian tonic called Winter Cherry, Indian Ginseng, Eshu and Ganda. It is a medicinal plant and has various effects, among which are the effects of tanning, stress relief, and immunity enhancement. Winter cherry roots have been used to relieve fever and inflammation from ancient times, and the main ingredient is a flavonoid, withanolide. It has been reported that it plays a role in antioxidant, antioxidant, immune function and cancer prevention. Recent studies have shown that applying cream containing winter cherry root extract to human body has anti-acne effect.

Coriandrum Sativum is the first spice used by mankind and is a medicinal plant used by doctors as medicines and remedies since ancient times. Origin is the eastern side of the Mediterranean Sea, but is now widely cultivated in China, India, Australia, the Soviet Union, and the Americas and is cultivated in warm and dry conditions. It is known that the Chinese eat most of the spices, and it is said to supplement the shortcomings of rich Chinese food. Spices have been used in everyday life of human beings from seeds, fruits, rootstocks, foliage, thorns, flowers and buds. It has the function of controlling the physiological activity of the human body as well as preserving and enhancing the quality of the product due to the direction action, antimicrobial and antioxidant action and the side effect of the product. It has anti-inflammatory and sedative properties, It has efficacy and is used medically. According to recent research results, high-purity water extract showed excellent antioxidant ability by phenolic compound, and anti-acne effect is also known.

Propolis is a substance made by mixing bees and enzymes in a resin-like substance extracted from various plants for survival and propagation. It contains organic substances, minerals, vitamins, amino acids, fats, organic acids and flavonoids. , Which play an important role in cell metabolism. The other ingredient, terpenes, is known to have anticancer effects, and propolis has been reported to have anti-inflammatory, antioxidant, and immunostimulatory effects and over 100 flavonoids. The antioxidant effect of propolis is due to the ability to inhibit prostaglandin-producing enzymes. The antioxidant effect is due to the active oxygen elimination effect of the major ingredient, flavonoid, and the anticancer effect is due to quercetin, which blocks the replication of cancer gene genes. have. Propolis is also called natural penicillin and is used as a raw material for antibacterial, antiviral, disinfectant, antifungal and antibiotic substances. In particular, it is known that antibiotics are effective against various bacteria such as Bacillus, Staphylococcus, Pseudomonas, Escherichia coli, Trichomonas, Salmonella, and it is known that propolis inhibits bacterial protein synthesis and plays an antibacterial function lost.

Prior art relating to the present invention relates to an antimicrobial composition comprising a natural raw material extract, a complex natural preservative, and a process for preparing the same, which discloses an antimicrobial composition comprising a water-extract, The present invention relates to an antimicrobial composition comprising an extract obtained by enzymatic treatment or fermentation treatment with various kinds of natural raw materials. The antimicrobial composition of the present invention is characterized in that the antimicrobial composition of the mixed extract composed of the sophorolipid, the winter cherry root, The constitution of the invention is different. In addition, Patent Document 10-2004-0033376 disclosing a cosmetic composition comprising a sophorolipid and Patent Document 10-2015-0050980 disclosing a pharmaceutical composition containing a winter cherry extract as an active ingredient, Each of the sophorolipids, winter cherries is a sole extract, and the antimicrobial function is not described so that the composition and purpose of the invention are different. Patent No. 10-0817781 on propolis extract discloses a cosmetic composition containing an extract of propolis alone as an active ingredient using tocopherol but the composition using a single extract using tocopherol is different from the present invention . Accordingly, an anti-acne functional cosmetic composition containing the mixed extract of the present invention as an active ingredient and a preparation method thereof are not disclosed or suggested in any of the above prior arts.

Accordingly, an object of the present invention is to provide an anti-acne functional cosmetic composition containing a soporolipid, a winter cherry root, a mixture of coriander and propolis as an active ingredient, and a process for producing the same.

The above object of the present invention can be attained by a method for the production of a medicament for the prevention and treatment of hepatocellular carcinoma, Gt; 60 C < / RTI > for 1 to 4 days;

The content of the mixed extract obtained through the step of obtaining a mixed extract obtained by mixing each of the soporolipid, winter cherry root, coriander, propolis extract and sophorolipid extract obtained in the above step in the same amount (w / w) By weight, based on the total weight of the cosmetic composition, of 1.67 to 100% by weight. The mixed extracts obtained above were achieved by evaluating the antimicrobial activity of growth inhibition of Propionibacterium acnes and the minimum inhibitory concentration by using a test material.

The present invention has an excellent effect of providing an anti-acne functional cosmetic composition containing a soporolipid, a winter cherry root, a coriander extract and a mixed extract containing an equal amount (w / w) of an extract of propolis as an active ingredient, and a process for producing the same .

Brief Description of the Drawings Fig. 1 is a photographic view showing a growth suspension of Propofolibacterium acnes of a soporolipid, a winter cherry root, a coriander and a propolis mixed extract according to the present invention.

The present invention provides an anti-acne functional cosmetic composition comprising a soporolipid, a winter cherry root, a mixture of coriander and propolis as an active ingredient, and a process for producing the same.

According to the present invention, the cosmetic composition has an excellent effect of inhibiting the growth of Propionibacterium acnes , an acne bacterium.

The cosmetic composition containing the extract prepared according to the present invention as an active ingredient can be prepared into any formulation conventionally produced in the art and can be formulated into, for example, lotion, lotion, cream and essence.

The present invention will be described in more detail with reference to the following specific examples, experimental examples and preparation examples. However, the following examples, experimental examples and preparation examples are for illustrative purposes only and are not intended to limit the scope of the present invention. It does not.

Example 1. Production of soporolipid, winter cherry root, coriander and propolis mixed extract of the present invention

In order to prepare a soporolipid, a winter cherry root, a coriander and a propolis mixed extract according to the present invention, the sophorolipid, the winter cherry root, the coriander and the propolis are dissolved in water, ethanol, methanol, butylene glycol, propanediol, At a ratio of 1: 10 to 1: 100 to the mixed solvent at 60 DEG C for 1 to 4 days;

Each of the winter cherry roots, coriander, propolis extract and sophorolipid extract obtained in the above step was blended in the same amount (w / w) to obtain a mixed extract.

Experimental Example 1 Growth Stability Measurements of Sophorolipid, Winter Cherry Root, High Water and Propolis Mixture Extracts by Concentration of the Extracts

Propionibacterium acnes was cultivated in a culture medium and cultivated at the Korean Microorganism Conservation Center for the measurement of growth stability by the concentration of the soporolipid, winter cherry root, coriander and propolis mixed extract of the present invention. The culture medium activated in the above step was dispensed into each of Patri dishes in an amount of 100 L each, and then placed on a paper disc (ADVANTEC 8 mm paper disc) to dissolve the samples. Then, the soporolipid, winter cherry root, The propolis mixed extract was diluted at a concentration ratio of 100% by weight, 50% by weight, 25% by weight and 12.5% by weight, and the mixture was divided into 25 uL portions. Ampicillin (50 ug / mL) was used as a positive control and BG40 was used as a negative control. Experiments were repeated three times.

As a result of the experiment, as shown in the following Tables 1 and 2, in the culture medium of Propionibacterium acnes, the same amount (w / w) of the present perilla lipid, winter cherry root, coriander and propolis extract When the mixed extract was dispensed at a concentration of 100% by weight, it was confirmed that growth of 18 ± 1 mm was stopped (FIG. 1).

Sample Propionibacterium acnes
(clear zone: mm) Experimental group (S) Anti acne natural formula (100%) 18 ± 1 Negative control (C) BG40 (solvent) - Positive control (Amp) Ampicillin 50 ug / mL 17 ± 1

Results by experimental group concentration 100 wt% 50 wt% 25 wt% 12.5 wt% Ampicillin 50 ug / mL P.acnes
(clear zone: mm) 18 ± 1 16 ± 1 16 ± 1 11 ± 1 17

Experimental Example 2 Minimum Inhibitory Concentration (MIC) measurement of Sophorolipid, Winter cherry root, coriander and propolis mixture extract of the present invention

The minimum inhibitory concentration (MIC) was measured to determine the antimicrobial activity against Propionibacterium acnes , a causative agent of acne. To this end, the culture broth obtained by adding the soporolipid, winter cherry root, mixed water and propolis mixed extract of the present invention obtained in Example 1 and cultivating Propionibacterium acnes was added to the growth medium to 1% After incubation, the cells were anaerobically cultured in a 10% CO ₂ incubator at 37 ° C for 48 hours, and then 100 μL was added to the solid medium. The growth of Propionibacterium acnes was confirmed. The experiment was repeated three times.

As a result of the experiment, as shown in the following Table 3, the mixed extract containing sophorolipid, winter cherry root, coriander and propolis extract of the present invention in the same amount (w / w) inhibited the growth of Propionibacterium acnes It was confirmed that the minimum concentration was 1.67% by weight.

MIC concentration (% by weight) The first experiment 1.5 Second experiment 2.0 Third experiment 1.5 Average 1.67

From the above experimental results, it was confirmed that the anticarcinogenic effect inhibiting the growth of Propionibacterium acnes, the acne bacterium of the present invention soporolipid, winter cherry root, high water and propolis mixed extract.

Hereinafter, various cosmetic formulations preferable for use of the present invention are disclosed, but it goes without saying that the scope of the present invention is not limited to the disclosed formulations.

Production Example 1 toilet water

The cosmetic lotion prepared by adding 10 wt% of the mixed extract containing the same amount (w / w) of the soporolipid, winter cherry root, high water and propolis mixed extract of the present invention obtained in Example 2 was prepared as shown in Table 4 .

Raw material Raw material Content (% by weight) One The present invention soporolipid, winter cherry root, coriander and propolis mixed extract 10.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Up to 100% by weight balance added

Production Example 2 Lotion

Lotions with 10% by weight of the mixed extracts containing the same amount (w / w) of the liposome of the present invention obtained in Example 1, winter cherry root, high water and mixed propolis extract were prepared as shown in Table 5 .

Raw material Raw material Content (% by weight) One The present invention soporolipid, winter cherry root, coriander and propolis mixed extract 10.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbidestalate 1.0 7 Chin type mono-stearic acid glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Up to 100% by weight balance added

Production Example 3 cream

10% by weight of a mixed extract of the present invention obtained in Example 1 containing the same amount (w / w) of lipofuscin, winter cherry root, coriander and propolis extract was prepared as shown in Table 6 below.

content Raw material Content (% by weight) One The present invention soporolipid, winter cherry root, coriander and propolis mixed extract 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Up to 100% by weight balance added

Production Example 4 Essence manufacturing

Essences containing 10% by weight of mixed extracts containing the same amount (w / w) of liposome, winter cherry root, coriander and propolis extract of the present invention obtained in Example 1 were prepared as shown in Table 7 below.

content Raw material Content (% by weight) One The present invention soporolipid, winter cherry root, coriander and propolis mixed extract 10.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Up to 100% by weight balance added

As described above, the anti-acne functional effect which suppresses the growth of Propionibacterium acnes which is an acne bacterium of the soporolipid, winter cherry root, coriander and propolis mixed extract of the present invention is excellent, This is a very useful invention.

Claims (4)

delete Ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof at a ratio of 1:10 to 1: 100 at room temperature for 1 to 4 days Gt; 60 C < / RTI >
(W / w) of each of the soporolipid, winter cherry root, coriander and propolis extract obtained in the above step, to obtain a cosmetic composition Wherein the composition is contained in an amount of 1.67 to 100% by weight based on the total weight of the cosmetic composition.
delete [Claim 3] The anti-acne functional cosmetic composition according to claim 2, wherein the cosmetic composition is any one selected from the group consisting of lotion, lotion, cream and essence.

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