KR20040033376A - Cosmetics composition comprising sophorolipids - Google Patents

Abstract

PURPOSE: Provided is a cosmetics composition comprising, as an active ingredient, sophorolipids which are produced from Candida bombiocola(ATCC 22214). The cosmetic composition has excellent sterilization effect on Propionibacterium acne, Staphylococcus, Micrococcus, Corynebacterium, Propionibacterium and the like, as well as moisturization and softening effects on the skin. CONSTITUTION: A cosmetics composition is characterized by comprising, as a biosurfactant, 0.01-10 wt.% of sophorolipids which are produced from Candida bombiocola(ATCC 22214). The composition is formulated into face lotion, nutritive emulsion, face cream and the like.

Description

Cosmetic composition containing phospholipids {COSMETICS COMPOSITION COMPRISING SOPHOROLIPIDS}

[Industrial use]

The present invention relates to a cosmetic composition comprising vesicle lipids, and more particularly, to a cosmetic composition comprising vesicle lipids, which are biosurfactants produced from microorganisms, as an active ingredient.

[Prior art]

Biosurfactant is a term encompassing all surfactants derived from living organisms, but often refers to surfactants produced by microorganisms. As the problems such as skin and human stability and biodegradation of synthetic surfactants and various environmental pollution problems caused by the increased use thereof, research and application of biosurfactants are increasing. This is because biosurfactants have high biodegradability and stability, unlike conventional synthetic surfactants, and may exhibit new surface chemical function and performance due to structural features not found in synthetic surfactants.

As an example of the biosurfactant in practical use, Japanese Kao Corp. (KAO Corp.) produces glycolipid surfactants from Torulopsis sp. Yeast to produce cosmetics as bioemulsifiers and skin moisturizers. In practical use, it is reported that a microbial polymer produced by a bacterium called Bacillus polymyxa is also used as a useful ingredient in cosmetics and shampoos.

On the other hand, acne refers to chronic inflammatory diseases of the hair follicle sebaceous glands characterized by the formation of comedones, papules, pustules, cysts, or nodules. The causes of acne are still not clearly identified, except that genetic and environmental factors may be directly involved in acne outbreaks. have. The first is sebum that is excessively secreted. The formation of sebum is caused by the action of male hormones, and the abnormal increase of this testosterone or various mechanisms associated with it causes sebaceous glands to become hyperfunctional, resulting in excess sebum. . Second is the closure of pores due to hyperkeratosis. The produced sebum should be discharged smoothly out of the skin, but the pores are blocked due to the exaggeration of the hair follicles, thereby suppressing the smooth discharge of sebum, and as a result, sebum is stagnated in the hair follicles to form microsurfaces. Third, due to the presence of propionibacterium acnes , this is explained as an important cause of acne lesion deterioration. As acne bacteria proliferate in the sebaceous glands, triglycerides that make up sebum are converted into free fatty acids and act as irritants or acne-producing substances. To promote acne production. It also progresses further and involves inflammatory reactions. Therefore, until now, evaluation for the purpose of alleviating acne has been carried out using a method of evaluating sebum secretion control, hyperkeratosis, and bactericidal activity against acne bacteria using the above-described mechanism of action.

In order to treat such acne in the past, attempts have been made to have a therapeutic effect by smoothing the secretion of sebum through exfoliation of pores using salicylic acid or vitamin A derivative retinoic acid preparations, or by using benzoyl peroxide preparations. Attempts have been made to cure through exfoliation and strong antimicrobial activity. However, the salicylic acid preparations may cause skin redness, swelling or skin respiratory disease as well as insignificant therapeutic effects. In the case of retinoic acid preparations, the effect of hyperkeratosis is suppressed, but contact dermatitis, erythema, dry skin, and exfoliation may occur. This can happen, and many side effects have also been reported for benzoyl peroxide preparations such as allergic contact dermatitis and scarring and severe erythema. In addition, the relatively recently introduced azelaci acid preparations have reduced drug side effects, but excellent clinical effects are not reported compared to conventional therapeutic products.

In addition, the body odor is generated by microorganisms decomposing eccrine sweat (apoccrine sweat), apocrine sweat (apocrine sweat), sebum, contaminants secreted by the human body. That is, the first secreted eccrine sweat or apocrine sweat causes odors such as odors, foot odors, and head odors caused by the action of various microorganisms, especially those associated with body odor, which are odorless or skin epidermis, hair, and pores. There are numerous floras on the skin, including Staphylococcus , Staphylococcus, Micrococcus , Corynebacterium , and Propionibacterium . Corynebacterium is known to be found in many strong odor holders due to nasal irritability. In addition, body odor is closely related to the distribution of microorganisms, and in the case of armpits, feet, and hair, there are more microorganisms than other parts. It turns out that it is because it is formed.

Various studies have been conducted as a method for suppressing the body odor by the antibacterial action among the methods that can cope with the human body odor, and synthetic materials such as triclosan and triclocarban are known as antibacterial substances. However, these synthetic antimicrobial agents are known to have excellent antibacterial effects, but have problems such as skin side effects.

In order to solve the problems of the prior art as described above, an object of the present invention is to provide a cosmetic composition excellent in antibacterial effect against acne bacteria.

Another object of the present invention is to provide a cosmetic composition excellent in antimicrobial effect against liquid odor bacteria.

Another object of the present invention is to provide a cosmetic composition excellent in skin moisturizing effect, feeling of use and skin softening effect.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a sopolori lipid produced from Candida bombiocola (ATCC 22214) as an active ingredient.

Hereinafter, the present invention will be described in detail.

In order to solve the problems of the prior art, the present inventors use skin bio-surfactant, a biosurfactant produced from Candida Bombbio Cola, which is not a synthetic chemical, as an active ingredient of a cosmetic composition. And excellent in the softening effect, confirming the antibacterial effect against acne bacteria and antibacterial effect against liquid odor bacteria was completed the present invention.

The biosurfactant producing microorganism used in the present invention uses Candida bombiocola , a kind of yeast. It is known that the candida bombio cola produces a lipophilic surfactant, a glycolipid-based surfactant, but other effects are still insignificant. Therefore, in the present invention, in addition to the role of the surfactant of the vesicle lipids, as well as the skin moisturizing effect and the bactericidal effect against acne and odor bacteria newly confirmed.

Sophorolipide used as an active ingredient in the cosmetic composition of the present invention can be obtained by inoculating and incubating passaged spawn seed in a high-pressure sterilized fermentor, followed by centrifugation and extraction.

Such phospholipids preferably have a structure represented by the following general formula (1) or (2).

[Formula 1]

[Formula 2]

Wherein R ₁ , R ₂ , and R ₃ are each independently or simultaneously hydrogen, or —COCH ₃ , and R ₄ is a straight or branched alkyl group having 1 to 20 carbon atoms.

In the present invention, it is preferable to use the content of the sorocoretides as an active ingredient in an amount of 0.01 to 10% by weight. When the content of the vesicle lipid is less than 0.01% by weight, the skin moisturizing effect, the feeling of use and the skin softening effect, the antibacterial effect of the acne bacteria and the liquid odor bacteria, which are the effect of the sorborelipid, are rarely exceeded and exceeded 10% by weight. This has no synergistic effect and tends to degrade the feel and feel of the skin.

The cosmetic composition comprising the vesicle lipid of the present invention as an active ingredient may be used by appropriately blending any ingredients blended into general skin cosmetics as needed. The optional components include oils, purified water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, sunscreens, antioxidants, dyes, fragrances and the like. For example, the cosmetic composition of the present invention is preferably formulated in the form of a lotion, nutrient, cream or pack, but is not limited thereto, and may be applied to various other formulations.

Hereinafter, the present invention will be described with reference to the following Examples and Comparative Examples. However, these examples are only for illustrating the present invention, and the present invention is not limited thereto.

EXAMPLE

Preparation Example 1

(Production of phospholipid)

Candida bombiocola ( Candyda bombiocola ) ATCC 22214 was used, the composition of the medium was as follows.

Medium composition: 3.0 g yeast extract, 10.0 g beef extract, 10.0 g peptone, 1.0 g soluble starch, 5.0 g glucose, 0.5 g L-cysteine hydrochloride, 5.0 g sodium chloride, 3.0 g sodium acetate, 0.75 g agar, pH 6.8

The seedlings passaged in a 100 ml flask for 48 hours were inoculated in a autoclaved 2.5 L fermenter and incubated for 7 days. After the incubation, the phospholipids were separated by centrifugation and extraction with ethyl acetate.

(Antibacterial activity evaluation experiment on acne bacteria)

Antimicrobial activity test against acne bacteria of Sopholori lipid obtained by the above method was inoculated to 10 ⁸ cell / ml concentration of Propionibacterium acnes pre-incubated in 10 ml BHI liquid medium. After culturing for 48 hours in an anaerobic incubator with a concentration of phophorolipid, the minimum inhibitory concentration was determined by measuring the number of P. acnes. As a result, the minimum inhibitory concentration range for Sophylori lipids against P. acnes was evaluated to be 150 to 200 ppm.

(Antibacterial activity evaluation experiment against liquid odor bacteria)

Corynebacterium xerosis ATCC 7711 was used as the liquid odor bacteria. Antimicrobial activity of Corynebacterium zerosis of Sophorolipid was pre-incubated in 10 ml of BHI liquid medium, followed by inoculation of Corynebacterium zerosis to a concentration of 10 ⁶ cells / ml After culturing for 48 hours in an aerobic incubator with a concentration of Sophorolipid, the minimum inhibitory concentration was determined by measuring the number of Corynebacterium zeroes. As a result, the minimum inhibitory concentration range of Sophorolipid against Corynebacterium zerosis was evaluated at 500 ppm.

Examples 1-2 and Comparative Example 1

Toner was prepared in the composition of the conventional lotion as shown in Table 1 and the composition to which the biosurfactant phophorolipid prepared in Preparation Example 1 was added. Glycerin, propylene glycol, a sunscreen and phospholipid were added to the purified water and dissolved at room temperature. On the other hand, oleyl alcohol, polyoxyethylene sorbitan monolauric acid ester, polyoxyethylene monolauryl ether, a preservative, and a fragrance | flavor were added to ethanol, and it melt | dissolved at room temperature, and it was solubilized by adding to the above-mentioned purified water. Thereafter, the mixture was colorized and filtered to produce a product.

Composition of lotion (unit: weight%) Raw material name Example 1 Example 2 Comparative Example 1 glycerin 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 Oleyl alcohol 0.1 0.1 0.1 Polyoxyethylene sorbitan monolauric acid ester 0.5 - 1.5 Polyoxyethylene Monolauryl Ether 0.5 0.5 0.5 Sopolori lipid 0.2 0.5 - ethanol 10.0 10.0 10.0 Spices 0.1 0.1 0.1 dyes Quantity Quantity Quantity Preservative, sunscreen Quantity Quantity Quantity Purified water Up to 100% by weight Up to 100% by weight Up to 100% by weight

Examples 3 to 4 and Comparative Example 2

A nutritive emulsion was prepared by adding a conventional nutritional fluid composition as shown in Table 2 below, and adding phospholipid, which is a biosurfactant prepared in Preparation Example 1. Propylene glycol and phospholipid were added to purified water, and the mixture was heated and mixed. Then, ethanol was added thereto and the temperature was raised to 70 ° C. The previously prepared aqueous solution of carboxyvinyl polymer and alkali were mixed and heated with the other lipophilic components to 70 ° C. Thereafter, the oil phase part was heated and uniformly mixed, neutralized by addition of alkali, uniformly emulsified by a homogenizer, and cooled to 30 ° C. by a heat exchanger.

Composition of nutritional milk (unit: weight%) Raw material name Example 3 Example 4 Comparative Example 2 Squalene 5.0 5.0 5.0 Waselin 2.0 2.0 2.0 Beeswax 0.5 0.5 0.5 Solbitan sesquioleic acid ester 0.8 0.8 0.8 Polyoxyethylene oleyl ether 0.5 - 1.2 Sopolori lipid 0.2 2.0 - Spices Quantity Quantity Quantity Antioxidant Quantity Quantity Quantity Propylene glycol 5.0 5.0 5.0 ethanol 5.0 5.0 5.0 Carboxy Vinyl Polymer 20.0 20.0 20.0 Potassium hydroxide 0.1 0.1 0.1 Purified water Up to 100% by weight Up to 100% by weight Up to 100% by weight

Examples 5-6 and Comparative Example 3

To prepare a cream with a composition of a conventional cream as shown in Table 3 and the composition added to the biosurfactant phophorolipid prepared in Preparation Example 1. Propylene glycol and phospholipid were added to the purified water, mixed, and then heated to 70 ° C. The remaining ingredients were mixed and dissolved by heating to 70 ° C., preliminarily emulsified in the aqueous phase, homogenized with a homogenizer and cooled to room temperature by a heat exchanger.

Composition of Cream (Unit: Weight%) Raw material name Example 5 Example 6 Comparative Example 3 Stearic acid 2.0 2.0 2.0 Stearyl alcohol 7.0 7.0 7.0 Reduced lanolin 2.0 2.0 2.0 Squalene 5.0 5.0 5.0 Octyldodecanol 6.0 6.0 6.0 Polyoxyethylene cetyl ether - 1.0 3.0 Lipophilic Monostearic Acid Glycerin 2.0 2.0 2.0 Sopolori lipid 2.0 0.2 - Spices Quantity Quantity Quantity Preservatives, Antioxidants Quantity Quantity Quantity Propylene glycol 5.0 5.0 5.0 Purified water Up to 100% by weight Up to 100% by weight Up to 100% by weight

Examples 7 to 8 and Comparative Example 4

To prepare a pack with the composition of the conventional pack as shown in Table 4 and the composition added to the biosurfactant phophorolipid prepared in Preparation Example 1. Solbite and sorbolipid were added to the purified water, mixed with titanium oxide, kaolin and an emulsion of vinyl oxide resin, and polyvinyl alcohol moistened with some ethanol was added thereto, followed by heating and dissolving at 70 ° C. Flavor and preservatives were added to the remaining alcohol and mixed, and finally, olive oil was added to cool the product.

Composition of the pack (unit: wt%) Raw material name Example 7 Example 8 Comparative Example 4 Vinyl acetate resin emulsion 15.0 15.0 15.0 Polyvinyl alcohol 10.0 10.0 10.0 olive oil 3.0 3.0 3.0 Solbit 3.0 0.5 5.0 Sopolori lipid 0.2 2.0 - Titanium oxide 8.0 8.0 8.0 kaoline 7.0 7.0 7.0 ethanol 5.0 5.0 5.0 Spices Quantity Quantity Quantity antiseptic Quantity Quantity Quantity Purified water Up to 100% by weight Up to 100% by weight Up to 100% by weight

Experimental Example 1

(Moisture retention test)

In order to determine the water retention ability of the vesicle lipids, the following experiment was conducted twice a day for 20 months on the male and female adults with dry hands. First, the skin moisture content measuring device (skin skin hygrometer), model name: SKICON-200, Japan IBS Product) and the results are shown in Table 5 below. The unit of measure used here is ㎲.

Moisture holding capacity measurement result division Average of measured values before application Average of measured values after application toilet water Example 1 2.5 19.0 Example 2 2.4 22.0 Comparative Example 1 2.6 18.0 Nutrition Example 3 2.5 18.2 Example 4 2.5 23.0 Comparative Example 2 2.6 13.4 cream Example 5 2.4 24.4 Example 6 2.4 26.5 Comparative Example 3 2.5 17.7 pack Example 7 2.6 8.3 Example 8 2.4 8.5 Comparative Example 4 2.5 7.0

As can be seen in Table 5, after applying the cosmetic of Examples 1 to 8 of the present invention on dry skin, the water retention capacity was increased. In addition, in the case of Examples 1 to 8 it can be seen that the moisture retention ability is further increased compared to the cosmetics of Comparative Examples 1 to 4 it is excellent in the skin moisturizing and softening effect.

Experimental Example 2

(Sterilization test on P. acnes)

In order to evaluate the antimicrobial activity of P. acnes of the cosmetic compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4, P. acne was added to a test tube containing 10 ml of sterile BHI liquid medium. Inoculated and incubated in an anaerobic incubator for 2 days, and the colony number (N1) of P. acnes was measured, and 1 g of 10% cosmetic solution of Examples and Comparative Examples was added to the test tube and then incubated in an anaerobic incubator for 1 day. Colony number N2 was measured. The bactericidal power against P. acnes was evaluated using the colony number obtained above. The bactericidal rate of P. acnes should be more than 90% in order to be effective. The bactericidal test results for P. acnes are shown in Table 6 below.

division First incubated blood, acnes number (N1) Blood after 3 days of applying cosmetics.Number of acnes (N2) Sterilization Rate (%) toilet water Example 1 1.50 × 10 ⁸ 1.50 × 10 ⁴ 99.99 Example 2 1.50 × 10 ⁸ 7.5 × 10 ⁴ 99.95 Comparative Example 1 1.50 × 10 ⁸ 1.8 × 10 ⁷ 88 Nutrition Example 3 1.30 × 10 ⁸ 6.5 × 10 ⁶ 95 Example 4 1.30 × 10 ⁸ 9.1 × 10 ⁶ 93 Comparative Example 2 1.30 × 10 ⁸ 3.25 × 10 ⁶ 75 cream Example 5 1.00 × 10 ⁸ 9.0 × 10 ⁶ 91 Example 6 1.00 × 10 ⁸ 9.5 × 10 ⁶ 90.5 Comparative Example 3 1.00 × 10 ⁸ 3.5 × 10 ⁷ 65 pack Example 7 1.70 × 10 ⁸ 1.36 × 10 ⁷ 92 Example 8 1.70 × 10 ⁸ 1.19 × 10 ⁷ 93 Comparative Example 4 1.70 × 10 ⁸ 4.93 × 10 ⁷ 71

In Table 6, it can be seen that in Examples 1 to 8, the sterilization rate for P. acnes was much better than that of Comparative Examples 1 to 4. In particular, in Examples 1 and 2, the sterilization rates for P. acres were 99.99 and 99.95%.

Experimental Example 3

(Sterilization test for Corynebacterium zerosis)

In order to evaluate the antimicrobial activity against Corynebacterium zerosis of the cosmetic compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4, sterilized BH liquid medium containing 0.1% of Tween-80 was added. Corynebacterium zerosis was inoculated into a test tube containing 10 ml, and cultured in an aerobic incubator for 2 days, and the colony count (N1) of Corynebacterium zerosis was measured, and 1 g of the cosmetics of Examples and Comparative Examples were added to the test tube. Colonies (N2) were measured after 1 day of incubation in an anaerobic incubator. The bactericidal activity against Corynebacterium zerosis was evaluated using the colony numbers obtained above. The sterilization rate of Corynebacterium zerosis should be more than 90% in order to be effective. Results of bactericidal power for Corynebacterium zerosis are shown in Table 7 below.

division Number of First-Generated Corynebacterium Zerosis (N1) Number of Corynebacterium zerosis 3 days after applying cosmetics (N2) Sterilization Rate (%) toilet water Example 1 2.0 × 10 ⁶ 2.0 × 10 ⁴ 99 Example 2 2.0 × 10 ⁶ 4.0 × 10 ⁴ 98 Comparative Example 1 2.0 × 10 ⁶ 3.0 × 10 ⁵ 85 Nutrition Example 3 4.0 × 10 ⁶ 2.7 × 10 ⁵ 93 Example 4 4.0 × 10 ⁶ 2.4 × 10 ⁵ 94 Comparative Example 2 4.0 × 10 ⁶ 9.2 × 10 ⁵ 77 cream Example 5 3.2 × 10 ⁶ 3.2 × 10 ⁵ 90 Example 6 3.2 × 10 ⁶ 2.88 × 10 ⁵ 91 Comparative Example 3 3.2 × 10 ⁶ 1.06 × 10 ⁶ 67 pack Example 7 5.6 × 10 ⁶ 4.48 × 10 ⁵ 92 Example 8 5.6 × 10 ⁶ 3.92 × 10 ⁵ 93 Comparative Example 4 5.6 × 10 ⁶ 1.68 × 10 ⁶ 70

In Table 7, it can be seen that in Examples 1 to 8, compared to Comparative Examples 1 to 4, the sterilization rate for Corynebacterium zerosis is excellent. In particular, in Example 1, the sterilization rate against Corynebacterium zerosis was 99, which was very good.

As described above, the present invention includes a microorganism produced by culturing natural microorganisms as a biosurfactant, the bactericidal effect, acne moisturizing effect on the acne and liquid odor bacteria, and the feeling of use and skin softness is very excellent Cosmetic compositions can be provided.

Claims (3)

Candida bombiocola ( Candida bombiocola ) (ATCC 22214) comprising a cosmetic composition comprising a vesicle lipids as an active ingredient. The method of claim 1, Cosmetic composition, characterized in that the content of the phosphorelipid is 0.01 to 10% by weight. The method of claim 1, Cosmetic composition, characterized in that the formulation of the cosmetic composition is a lotion, nutrient fluid, cream or lotion.