WO2024115213A1

WO2024115213A1 - Detergent compartment pouch comprising biosurfactants

Info

Publication number: WO2024115213A1
Application number: PCT/EP2023/082639
Authority: WO
Inventors: Konrad GRYGIEL; Alexandra Trambitas; Kurt Seidel; Jakob Müller; Jochen Kleinen
Original assignee: Evonik Operations Gmbh
Priority date: 2022-11-30
Filing date: 2023-11-22
Publication date: 2024-06-06

Abstract

The invention relates to detergent compartment pouches comprising biosurfactants and a water-soluble film comprising polyvinyl alcohol.

Description

Detergent compartment pouch comprising biosurfactants

Field of the invention

The invention relates to detergent compartment pouches comprising biosurfactants and a water- soluble film comprising polyvinyl alcohol.

Prior art

Detergent products in unit dose form have become one of the preferred forms for the user due to the easiness of use, in particular water-soluble pouches which present the added advantage of no need to unwrap.

Usually it is desired, that the water-soluble pouches are filled with liquid detergent solutions, which limits the variety of suited detergent compositions to be filled into the pouches.

Quite frequently some of the liquid might “sweat” through the water-soluble film, which yields in a sticky surface.

Also, some liquids may diffuse through the water-soluble film into a second compartment filled with powder compositions, which consequently agglutinate and thus do not dissolve fast and easily in the suds while washing.

Therefore, the water content of the contained liquid detergent usually is reduced to a minimum and/or non-aqueous solvents are used as liquefiers limiting the amount of suited detergent compositions even further.

Also, the thickness of the water-soluble film often is increased in order to have a more stable pouch, which, however, consumes more resources, which do not contribute to the washing efficiency of the product.

Furthermore, for safety reasons, it is desirable to formulate unit dose detergents, that do not dissolve in water too fast, to avoid swallowing of the detergent content when small kids are tempted to put the pouch into their mouths by the often-colourful product.

Thus, there is a need to provide detergent compartment pouches with a water-soluble film being quite resistant to the contained liquid detergent solution.

The object of the present invention is to design a detergent product which obviates the above challenges. Description of the invention

It was found that, surprisingly, that detergent compartment pouches with water-soluble films comprising polyvinyl alcohol (PVOH) and containing biosurfactants have outstanding properties regarding their stickiness during long time storage.

The present invention therefore provides a detergent compartment pouch having at least one water-soluble film forming at least one compartment, wherein the at least one compartment contains a liquid composition, said liquid composition comprises at least one biosurfactant, characterized in that said water-soluble film comprises polyvinyl alcohol.

The invention further provides the use of a pouch according to the instant invention for cleaning a surface of an article,

One advantage of the present invention is that the detergent compartment pouch of the instant invention exhibits improved integrity and resistance to water.

An advantage of the present invention is a better storage stability of the detergent compartment pouch of the instant invention.

A further advantage of the detergent compartment pouch of the instant invention is that the pouches can make use of a thinner water-soluble film, thereby saving packaging material.

Another advantage of the detergent compartment pouch of the instant invention is, that the contained liquid composition may be formulated without addition of ethanolamine while still preserving its pourable liquid character.

A further advantage of the instant invention is, that dyes comprised in the detergent compartment pouch according to the instant invention exhibit an enhanced colour stability.

Another advantage of the detergent compartment pouch of the instant invention is, that dyes comprised in the detergent compartment pouch according to the instant invention do not transfer to textiles during washing processes.

A further advantage of the instant invention is, that dyes comprised in the detergent compartment pouch according to the instant invention prevent children to taste the detergent pouch.

A further advantage of the instant invention is, that less dye can be incorporated in the detergent compartment pouch according to the instant invention without significant loss of brilliance of the dye.

The present invention therefore provides a detergent compartment pouch having at least one water-soluble film forming at least one compartment, wherein the at least one compartment contains a liquid composition, said liquid composition comprises at least one biosurfactant, characterized in that said water-soluble film comprises polyvinyl alcohol. Within the context of the present invention, “biosurfactants” are understood as meaning all glycolipids produced by fermentation. The term “biosurfactant” also covers glycolipids that are chemically or enzymatically modified after fermentation, as long as structurally a glycolipid remains. Raw materials for producing the biosurfactants that can be used are carbohydrates, in particular sugars such as e.g. glucose and/or lipophilic carbon sources such as fats, oils, partial glycerides, fatty acids, fatty alcohols, long-chain saturated or unsaturated hydrocarbons.

In the context of the present invention, the terms “surfactant” is understood to mean organic substances having interface-active properties that have the ability to reduce the surface tension of water at 20°C and at a concentration of 0.5% by weight based on the overall composition to below 45 mN/m. Surface tension is determined by the Du Noliy ring method at 20°C.

In the context of the present invention, the terms “liquid” is understood to be liquid at a temperature of 25°C and a pressure of 1013 mbar.

Where average values are stated hereinbelow, then, unless stated otherwise, these are number- averaged average values.

Unless stated otherwise, percentages are data in per cent by weight.

Wherever measurement values are stated hereinbelow, then, unless stated otherwise, these have been determined at a temperature of 25°C and a pressure of 1013 mbar.

The pouch according to the instant invention preferably is characterized in that said biosurfactant is selected from rhamnolipids, sophorolipids, glucolipids, cellulose lipids, mannosylerythritol lipids and trehalose lipids, preferably rhamnolipids, sophorolipids and glucolipids, most preferably rhamnolipids.

Mixtures of different biosurfactants can be comprised in the liquid composition, of course.

The biosurfactants can be produced e.g. as in EP 0 499 434, US 7,985,722, WO 03/006146, JP 60 183032, DE 19648439, DE 19600743, JP 01 304034, CN 1337439, JP 2006 274233,

KR 2004033376, JP 2006 083238, JP 2006 070231 , WO 03/002700, FR 2740779, DE 2939519, US 7.556,654, FR 2855752, EP 1445302, JP 2008 062179 and JP 2007 181789 or the documents cited therein. Suitable biosurfactants can be acquired e.g. from Soliance, France.

Preferably, the pouch according to the instant invention has as biosurfactant at least one selected from rhamnolipids, in particular mono-, di- or polyrhamnolipids, glucolipids, in particular mono-, di- or polyglucolipids, and sophorolipids, in particular mono-, di- or polysophorolipids, preferably selected from rhamnolipids and glucolipids, most preferably rhamnolipids.

The term "rhamnolipids" in the context of the present invention preferably is understood to mean particularly compounds of the general formula (I) and salts thereof,

where mRL = 2, 1 or 0, preferably 1 or 0, nRL = 1 or 0,

R^1RL and R^2RL = mutually independently, identical or different, organic residues having 2 to 24, preferably 5 to 13 carbon atoms, in particular optionally branched, optionally substituted, particularly hydroxy-substituted, optionally unsaturated, in particular optionally mono-, bi- or triunsaturated alkyl residues, preferably those selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH2)o-CH3 where o = 1 to 23, preferably 4 to 12. If nRL = 1 , the glycosidic bond between the two rhamnose units is preferably in the a-configuration. The optically active carbon atoms of the fatty acids are preferably present as R-enantiomers (e.g. (R)-3-{(R)-3-[2-0-(a-L-rhamnopyranosyl)-a-L-rhamnopyranosyl]oxydecanoyl}oxydecanoate).

The term "di-rhamnolipid" in the context of the present invention is understood to mean compounds of the general formula (I) or salts thereof, where nRL = 1 .

The term "mono-rhamnolipid" in the context of the present invention is understood to mean compounds of the general formula (I) or salts thereof, where nRL = 0.

Distinct rhamnolipids are abbreviated according to the following nomenclature: "diRL-CXCY" are understood to mean di-rhamnolipids of the general formula (I), in which one of the residues R^1RL and R^2RL = (CH2)o-CH3 where o = X-4 and the remaining residue R¹ or R² = (CH₂)O-CH₃ where o = Y-4.

"monoRL-CXCY" are understood to mean mono-rhamnolipids of the general formula (I), in which one of the residues R^1RL and R^2RL = (CH2)o-CH3 where o = X-4 and the remaining residue R^1RL or R^2RL = (CH₂)O-CH₃ where o = Y-4.

The nomenclature used therefore does not distinguish between "CXCY" and "CYCX". For rhamnolipids where mRL=0, monoRL-CX or diRL-CX is used accordingly. If one of the abovementioned indices X and/or Y is provided with ":Z", this signifies that the respective residue R^1RL and/or R^2RL is equal to an unbranched, unsubstituted hydrocarbon residue having X-3 or Y-3 carbon atoms having Z double bonds.

Methods for preparing the relevant rhamnolipids are disclosed, for example, in EP2786743 and EP2787065.

Rhamnolipids applicable in the context of the instant invention can also be produced by fermentation of Pseudomonas, especially Pseudomonas aeruginosa, which are preferably non genetically modified cells, a technology already disclosed in the eighties, as documented e.g. in EP0282942 and DE4127908. Rhamnolipids produced in Pseudomonas aeruginosa cells which have been improved for higher rhamnolipid titres by genetical modification can also be used in the context of the instant invention; such cells have for example been disclosed by Lei et al. in Biotechnol Lett. 2020 Jun;42(6):997-1002.

Rhamnolipids produced by Pseudomonas aeruginosa are commercially available from Jeneil Biotech Inc., e.g. under the tradename Zonix.from Logos Technologies (technology acquired by Stepan), e.g. under the tradename NatSurFact, from Biotensidion GmbH, e.g. under the tradename Rhapynal, from AGAE technologies, e.g. under the name R90, R95, R95Md, R95Dd, from Locus Bio-Energy Solutions and from Shanghai Yusheng Industry Co. Ltd., e.g. under the tradename Bio- 201 Glycolipids.

The present invention provides a pouch preferably comprising in said liquid composition as said biosurfactant rhamnolipids, characterized in that the rhamnolipids comprise

51 % by weight to 100% by weight, preferably 60% by weight to 95% by weight, particularly preferably 80% by weight to 90% by weight, of mono-rhamnolipids, especially those of formula (I) with nRL=0, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

The present invention provides a pouch alternatively preferably comprising in said liquid composition as said biosurfactant rhamnolipids, characterized in that the rhamnolipids comprise 71 % by weight to 100% by weight, preferably 75% by weight to 95% by weight, particularly preferably 80% by weight to 90% by weight, of di-rhamnolipids, especially those of formula (I) with nRL=1 , where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

The present invention further provides a pouch preferably comprising in said liquid composition as said biosurfactant rhamnolipids, characterized in that the rhamnolipids comprise

56% by weight to 95% by weight, preferably 60% by weight to 80% by weight, particularly preferably 66% by weight to 70% by weight, of diRL-C10C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

A preferred pouch according to the invention is characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of 0.5% by weight to 15% by weight, preferably 3% by weight to 12% by weight, particularly preferably 5% by weight to 10% by weight, of diRL-C10C12:1 , where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

A further preferred pouch according to the invention is characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of 0.5 to 25% by weight, preferably 3% by weight to 15% by weight, particularly preferably 5% by weight to 12% by weight, of diRL-C10C12, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

A preferred pouch according to the invention is characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of 0.1 % by weight to 25% by weight, preferably 2% by weight to 10% by weight, particularly preferably 4% by weight to 8% by weight, of diRL-C8C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

An even further preferred pouch according to the invention is characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of

0.1 % by weight to 5% by weight, preferably 0.5% by weight to 3% by weight, particularly preferably 0.5% by weight to 2% by weight, of monoRL-C8C10 and/or, preferably and

0.1 % by weight to 5% by weight, preferably 0.5% by weight to 3% by weight, particularly preferably 0.5% by weight to 2% by weight, of monoRL-C10C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

The present invention provides a pouch alternatively preferably comprising in said liquid composition as said biosurfactant rhamnolipids, characterized in that the rhamnolipids comprise

10% by weight to 30% by weight, preferably 20% by weight to 30% by weight, particularly preferably 25% by weight to 30% by weight, of monoRL-C10C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

The alternatively preferred pouch according to the invention is preferably characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of 10 % by weight to 30 % by weight, preferably 12% by weight to 25 % by weight, particularly preferably 15% by weight to 20% by weight, of diRL-C10C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

The alternatively preferred pouch according to the invention is preferably characterized in that the pouch comprises in said liquid composition as said biosurfactant rhamnolipids as described above with a content of

10 % by weight to 30 % by weight, preferably 12% by weight to 25 % by weight, particularly preferably 15% by weight to 20% by weight, of monoRL-C8C10, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

3% by weight to 25% by weight, preferably 5% by weight to 20% by weight, particularly preferably 10% by weight to 15% by weight, of monoRL-C10C12:1 , where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

1 % by weight to 15% by weight, preferably 2% by weight to 10% by weight, particularly preferably 3% by weight to 8% by weight, of diRL-C10C12, where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

Rhamnolipids produced by Pseudomonas aeruginosa are commercially available from Jeneil Biotech Inc., e.g. under the tradename Zonix.from Logos Technologies (technology acquired by Stepan), e.g. under the tradename NatSurFact, from Biotensidion GmbH, e.g. under the tradename Rhapynal, from AGAE technologies, e.g. under the name R90, R95, R95Md, R95Dd, from Locus Bio-Energy Solutions and from Shanghai Yusheng Industry Co. Ltd., e.g. under the tradename Bio- 201 Glycolipids. In the context of the present invention, the term “sophorolipids” preferably is understood as meaning compounds of the general formulae (Ila) and (lib) and salts thereof

where R^1SL = H or CO-CH₃,

R^2SL = H or CO-CH₃, R₃SI_ _{= a} di_va|_ent organic moiety which comprises 6 to 32 carbon atoms and which is unsubstituted or substituted by hydroxyl functions, is unbranched and optionally comprises one to three double or triple bonds,

R4SI_ ₌ |_| c|_|_{3 or a} monovalent organic radical which comprises 2 to 10 carbon atoms and which is unsubstituted or substituted by hydroxyl functions, which is unbranched and which optionally comprises one to three double or triple bonds, and nSL = 1 or 0.

Sophorolipids may be used in accordance with the invention in their acid form or their lactone form. Preferred compositions according to the instant invention comprise a sophorolipid in which the ratio by weight of lactone form to acid form is in the range of 20:80 to 80:20, especially preferably in the ranges of 30:70 to 40:60.

To determine the content of sophorolipids in the acid or lactone form in a formulation, refer to EP1411111 B1 , page 8, paragraph [0053],

In connection with the present invention, the term “glucolipids” preferably is understood as meaning compounds of the general formula (III) and salts thereof,

where mGL = 3, 2, 1 or 0, preferably 1 or 0,

R^1GL and R^2GL = independently of one another identical or different organic radical having 2 to 24 carbon atoms, in particular optionally branched, optionally substituted, in particular hydroxysubstituted, optionally unsaturated, in particular optionally mono-, di- or triunsaturated, alkyl radical, preferably one selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH2)o-CH₃ where o = 1 to 23, preferably 4 to 12.

Distinct glucolipids are abbreviated according to the following nomenclature:

“GL-CXCY” is understood as meaning glucolipids of the general formula (III) in which one of the radicals R^1GL and R^2GL = (CH2)o-CH₃ where o = X-4 and the remaining radical R^1GL or R^2GL = (CH2)o- CH₃ where o = Y-4.

The nomenclature used thus does not differentiate between “CXCY” and “CYCX”. If one of the aforementioned indices X and/or Y is provided with “:Z”, then this means that the respective radical R^1GL and/or R^2GL = an unbranched, unsubstituted hydrocarbon radical with X-3 or Y-3 carbon atoms having Z double bonds.

Methods for production of glucolipids can be carried out as described in WO2019154970.

A preferred pouch according to the instant invention is characterized in that the biosurfactant is comprised in said liquid composition in an amount of 0.1 wt.-% to 50 wt.-%, preferably 1 .0 wt.-% to 20 wt.-%, more preferably 5.0 wt.-% to 15 wt.-%, where the percentages by weight refer to the total liquid composition.

When determining the content of biosurfactant in the context of the present invention, the mass of the non-salt form is taken into account; thus, the weight of the corresponding cation is disregarded.

A preferred pouch according to the instant invention is characterized in that it comprises in said liquid composition at least one non-biosurfactant, preferably selected from the group of anionic, cationic, non-ionic, semi-polar, amphoteric and zwitterionic surfactants.

A preferred pouch according to the instant invention is characterized in that the biosurfactant is comprised in said liquid composition in an amount of 50 wt.-% to 100 wt.-%, preferably 60 wt.-% to 98 wt.-%, more preferably 80 wt.-% to 95 wt.-%, where the percentages by weight refer to the sum of all surfactants comprised in the liquid composition.

Preferably the non-biosurfactant is selected from the group of fatty alcohol alkoxylates. These can be advantageously used for cleaning a surface of a textile or a fabric containing polyamines.

Non-limiting examples of anionic surfactants include sulfates and sulfonates, in particular, linear alkylbenzenesulfonates (LAS), isomers of LAS, branched alkylbenzenesulfonates (BABS), phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates, alkane- 2,3-diylbis(sulfates), hydroxyalkanesulfonates and disulfonates, alkyl sulfates (AS) such as sodium dodecyl sulfate (SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), alcohol ethersulfates (AES or AEOS or FES, also known as alcohol ethoxysulfates or fatty alcohol ether sulfates), secondary alkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES) including methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters of sulfo-succinic acid or soap, and combinations thereof. Non-limiting examples of cationic surfactants include alklydimethylethanolamine quat (ADMEAQ), cetyltrimethylammonium bromide (CTAB), dimethyldistearylammonium chloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternary ammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, and combinations thereof.

Non-limiting examples of non-ionic surfactants include alcohol ethoxylates (AE or AEO), which are preferably comprised in the composition according to the instant invention, alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters, such as ethoxylated and/or propoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkylpolyglycosides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), polyglycerol esters, glycerol esters, propoxylated fatty acid monoethanolamides (PFAM), polyhydroxy alkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, or fatty acid glucamide, FAGA), as well as products available under the trade names SPAN and TWEEN, and combinations thereof.

Non-limiting examples of semipolar surfactants include amine oxides (AO) such as alkyldimethylamineoxide, N-(coco alkyl)-N,N-dimethylamine oxide and N-(tallow-alkyl)-N,N-bis(2- hydroxyethyl)amine oxide, fatty acid alkanolamides and ethoxylated fatty acid alkanolamides, and combinations thereof.

Non-limiting examples of amphoteric and zwitterionic surfactants include betaine, alkyldimethylbetaine, sulfobetaine, hydroxysultaine and combinations thereof.

Furthermore, bio-based surfactants can be included in the composition according to the instant invention, which are not based on glycolipids, for example lipopeptides like surfactins or phospholipids like lecithins.

A preferred pouch according to the instant invention is characterized in that said liquid composition comprises at least one water soluble dye.

The term “water soluble” in the context of the instant invention means, that the substance is soluble in water at a concentration of at least 10 mg/l at 25 °c and 1 bar.

A preferred pouch according to the instant invention is characterized in that said water soluble dye is selected from the group comprising, preferably consisting of, acid blue 1 (also known as Cl 42045, hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium), acid blue 3, (also known as patent blue V, Cl 42051 , exemplary salt is (bis[hydrogen [4-[4- (diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1- ylidene]diethylammonium], calcium salt), acid blue 7 (also known as Cl 42080, exemplary salt is hydrogen (benzyl)[4-[[4-

[benzylethylamino]phenyl](2,4-disulphonatophenyl)methylene]cyclohexa-2,5-dien-1- ylidene](ethyl)ammonium, sodium salt), acid blue 145 (also known as C.l. 62070, disodium 1-amino-9,10-dihydro-4-[(4-methyl-2- sulphonatophenyl)amino]-9, 10-dioxoanthracene-2-sulphonate), acid blue 182 (Disodium 4-[[4-(acetylmethylamino)-2-sulphonatophenyl]amino]-1-amino-9, 10- dihydro-9, 10-dioxoanthracene-2-sulphonate), acid blue 185 (also known as C.l.74200, phthalocyanine class), acid blue 193 (also known as C.l. 15707, disodium hydrogen bis[3-hydroxy-4-[(2-hydroxy-1- naphthyl)azo]naphthalene-1-sulphonato(3-)]chromate(3-)),

C.l. acid blue 9 (also known as Cl 42090, exemplary salt is dihydrogen(ethyl)[[4-[4-[ethyl(3- sulfonatobenzyl)]amino]-2'-sulfonatobenzhydryliden]cyclohexa-2,5-dien-1-yliden]-(3- sulfonatobenzyl)ammonium, sodium salt),

C.l. acid blue 93 (also known as Cl 42780, disodium [[4-[bis[4-

[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1- ylidene]amino]benzenesulphonate), 1 ,4-bis(methesitylamino)anthraquinone),

C.l. pigment blue 29 (also known as Cl 77007, exemplary salt is silicic acid, aluminum sodium salt, sulfurized),

C.l. solvent blue 3 (4-[{4-(anilino) phenyl]-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]- 2-Methylaniline}), and

C.l. reactive blue 116, preferably acid blue 1 (also known as Cl 42045, hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium), acid blue 3, (also known as patent blue V, Cl 42051 , exemplary salt is (bis[hydrogen [4-[4- (diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1- ylidene]diethylammonium], calcium salt), acid blue 145 (also known as C.l. 62070, disodium 1-amino-9,10-dihydro-4-[(4-methyl-2- sulphonatophenyl)amino]-9, 10-dioxoanthracene-2-sulphonate), acid blue 182 (Disodium 4-[[4-(acetylmethylamino)-2-sulphonatophenyl]amino]-1-amino-9, 10- dihydro-9, 10-dioxoanthracene-2-sulphonate),

C.l. pigment blue 29 (also known as Cl 77007, exemplary salt is silicic acid, aluminum sodium salt, sulfurized), and

C.l. solvent blue 3 (4-[{4-(anilino) phenyl]-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]- 2-Methylaniline}), most preferably; acid blue 1 (also known as Cl 42045, hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium), acid blue 3, (also known as patent blue V, Cl 42051 , exemplary salt is (bis[hydrogen [4-[4- (diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1- ylidene]diethylammonium], calcium salt), acid blue 145 (also known as C.l. 62070, disodium 1-amino-9,10-dihydro-4-[(4-methyl-2- sulphonatophenyl)amino]-9, 10-dioxoanthracene-2-sulphonate), acid blue 182 (Disodium 4-[[4-(acetylmethylamino)-2-sulphonatophenyl]amino]-1-amino-9, 10- dihydro-9, 10-dioxoanthracene-2-sulphonate), and

C.l. pigment blue 29 (also known as Cl 77007, exemplary salt is silicic acid, aluminum sodium salt, sulfurized).

The dye preferably is comprised in an amount of 0.001 wt.-% to 1.0 wt.-%, preferably 0.01 wt.-% to 0.5 wt.-%, more preferably 0.1 wt.-% to 0.25 wt.-%, where the percentages by weight refer to the total liquid composition.

A preferred pouch according to the instant invention is characterized in that said liquid composition comprises water in an amount of 0.1 wt.-% to 25 wt.-%, preferably 8.0 wt.-% to 20 wt.-%, more preferably 16 wt.-% to 19 wt.-%, where the percentages by weight refer to the total liquid composition.

A preferred pouch according to the instant invention is characterized in that said liquid composition comprises monoethanolamine (MEA) in an amount of less than 15 wt.-%, preferably less than 10 wt.-%, more preferably 0.01 wt.-% to 4.0 wt.-%, where the percentages by weight refer to the total liquid composition. Preferably said liquid composition comprises no detectable amount of MEA.

The pouch according to the instant invention has a water-soluble film comprising polyvinyl alcohol. The polyvinyl alcohol can be partially acetalized or be present in the form of polyvinyl alcohol copolymers.

Polyvinyl alcohols suited to be comprised in the water-soluble film are commercially available, for example under the trade name Solublon® (AICELLO-HARKE), Mowiol® (Clariant); water-soluble films comprising polyvinyl alcohol are commercially available, for example under the trade name Watersol™ and MonoSol (Kuraray). Polyvinyl alcohols which are particularly suitable in the context of the present invention are, for example, Solublon® GS, Solublon® GA, Solublon® PT, Solublon® KC, Solublon® KL, Mowiol® 3-83, Mowiol® 4-88, Mowiol® 5-88, Mowiol® 8-88 and Clariant L648. A polyvinyl alcohol preferably comprised in the water-soluble film of the pouch of the instant invention has a degree of hydrolysis of 70 mol %to 100 mol %, preferably 80 mol % to 90 mol %, particularly preferably 81 mol %to 89 mol %, and in particular 82 mol % to 88 mol %.

A polyvinyl alcohol preferably comprised in the water-soluble film of the pouch of the instant invention has a molecular weight in the range from 10,000 g/mol to 100,000 g/mol, preferably from 1 ,000 g/mol to 90,000 g/mol, particularly preferably from 12,000 g/mol to 80,000 g/mol and in particular from 14,000 g/mol to 68,000 g/mol.

The water-soluble film of the pouch of the instant invention may optionally comprise additional polymers selected from the group comprising acrylic acid-containing polymers, polyacrylamides, oxazoline polymers, polystyrenesulfonates, polyurethanes, polyesters, polyethers and/or mixtures of the above.

A water-soluble film of the pouch of the instant invention preferably comprises said polyvinyl alcohol in an amount of at least 50 wt.-%, preferably of at least 70 wt.-%, particularly preferably of at least 80 wt.-% and in particular of at least 92 wt.-%, where the percentages by weight refer to the total weight of the water-soluble film.

The pouch according to the instant invention can be detergent multi-compartment pouch having more than just one compartment.

The different compartments can be filled with different compositions, as long as at least one of the compartments contains a liquid composition, said liquid composition comprising at least one biosurfactant as described above.

Preferably, at least one of the compartments in the detergent multi-compartment pouch according to the instant invention contains a solid composition and whereas at least on other compartment contains said liquid composition. The compositions are preferably in a solid to liquid weight ratio of from about 20:1 to about 1 :20, more preferably from about 18:1 to about 2:1 and even more preferably from about 15:1 to about 5:1 . The detergent multi-compartment pouch according to the instant invention is very versatile because it can accommodate compositions having a broad spectrum of values of solickliquid ratio. Particularly preferred have been found to be detergent multi-compartment pouches having a high solid: liquid ratio because many of the detergent ingredients are most suitable for use in solid form, preferably in powder form. The ratio solid:liquid defined herein refers to the relationship between the weight of all the solid compositions and the weight of all the liquid compositions in the pouch.

Alternatively preferred in detergent multi-compartment pouches according to the instant invention the solid: liquid weight ratio is from about 1 :2 to about 1 :18, more preferably from about 1 :5 to about 1 :15. These weight ratios are suitable in cases in which most of the ingredients of the detergent are in liquid form.

It was surprisingly found that diffusion of liquid from one compartment to the compartments) containing solid composition(s) is greatly diminished. Thus, solid compositions contained in the form of flowing powders do not agglutinate.

Enzymes are useful additives in washing compositions, like for example dish washing or laundry compositions.

The detergent compartment pouch according to the instant invention preferably comprises at least one enzyme. Preferably said enzyme is comprised in the liquid composition comprising the at least one biosurfactant.

The enzymes preferably comprised in the pouch according to the instant invention are selected from the group consisting of protease, amylase, lipase, pectinase, cellulase, phosphodiesterase, mannanase, cutinase, pectate lyase, peroxidase, oxidase and laccase with protease, amylase, lipase, pectinase, cellulase, phosphodiesterase and mannanase being especially preferred.

The enzymes to be used in the context of the present invention can, for example, originally originate from microorganisms, for example of the genera Bacillus, Streptomyces, Humicola, or Pseudomonas, and I or be produced by suitable microorganisms according to known biotechnological processes, for example by transgenic expression hosts, for example of the genera Escherichia, Bacillus, or filamentous fungi.

It is emphasized that it can in particular also be technical enzyme preparations of the respective enzyme, i.e. accompanying substances may be present. The enzymes can therefore be packaged and used together with accompanying substances, for example from fermentation or with other stabilizers.

Suitable proteases include those of bacterial, fungal, plant, viral or animal origin e.g. vegetable or microbial origin. Microbial origin is preferred. Chemically modified or protein engineered mutants are included. It may be an alkaline protease, such as a serine protease or a metalloprotease. A serine protease may for example be of the S1 family, such as trypsin, or the S8 family such as subtilisin. A metalloproteases protease may for example be a thermolysin from e.g. family M4 or other metalloprotease such as those from M5, M7 or M8 families.

Examples of proteases are the subtilisins BPN from Bacillus amyloliquefaciens and Carlsberg from Bacillus licheniformis, the protease PB92, the subtilisins 147 and 309, the protease from Bacillus lentus, subtilisin DY and the subtilases, but no longer assigned to the subtilisins in the narrower sense Thermitase, Proteinase K and the proteases TW3 and TW7.

Subtilisin Carlsberg is in a further developed form under the trade name Alcalase® from the Novozymes A / S, Bagsvasrd, Denmark. The subtilisins 147 and 309 are sold by the Novozymes company under the trade names Esperase® and Savinase®, respectively. The protease variants under the name BLAP® are derived from the protease from Bacillus lentus DSM 5483. Further usable proteases are, for example, those under the trade names Durazym®, Relase®, Everlase®, Nafizym®, Natalase®, Kannase® and Ovozyme® from Novozymes, which are among trade names, Purafect®, Purafect® OxP, Purafect® Prime, Excellase® and Properase® from the company Danisco I Genencor, which operates under the trade name Protosol® from Company Advanced Biochemicals Ltd., Thane, India, which operates under the trade name Wuxi® from the company Wuxi Snyder Bioproducts Ltd., China, which operates under the trade name Proleather® and Protease P® from Amano Pharmaceuticals Ltd., Nagoya, Japan, and the enzyme available under the name Proteinase K-16 from Kao Corp., Tokyo, Japan.

The proteases from Bacillus gibsonii and Bacillus pumilus, which are disclosed in the international patent applications WO 08/086916 and WO 07/131656, are also particularly preferably used. Further advantageously usable proteases are disclosed in the patent applications WO 91/02792, WO 08/007319, WO 93/18140, WO 01/44452, GB 1243784, WO 96/34946, WO 02/029024 and WO 03/057246. Other proteases that can be used are those found in the microorganisms Stenotrophomonas maltophilia, in particular Stenotrophomonas maltophilia K279a, Bacillus intermedius and Bacillus sphaericus are naturally present.

Other commercially available proteases are: Liquanase® EC 3.5 L, Liquanase® Evity® EC 3.5 L, Liquanase® 3.5 L, Liquanase® Evity® 3.5 L, Preferenz P100, Preferenz P200, Preferenz P300, Biotouch ROC, BIOPROTEASA L 800 ST, Bioproteasa 800 P, Bioproteasa L 800, Lavergy® Pro 114 LS, Progress Uno EC 100 L, Progress Uno 100 L, Progress Uno 101 L, EFFECTENZ™ P 100 (A01339), EFFECTENZ™ P 150, Savinase® Evity® EC 16 L, Savinase® Evity® EC 24 T, Savinase® Evity® 16 L, Savinase® Evity® 24 T, Excellenz P 1250, Blaze® Evity® EC 150 T, Blaze® Evity® 150 T, Blaze® Evity® 125 T, Blaze Evity 16 L, Excellase, Purafect, Purafect OxP, Purafect Prime, Properase, Blaze® Pro EC 100 L, Blaze® Pro 100 L, Blaze Exceed 100 T, Progress Key 150 T, Progress Excel 101 L.

A protease preferably comprised in the pouch according to the instant invention is Liquanase® 2.5 L.

Suitable amylases which can be used herein may be an alpha-amylase or a glucoamylase and may be of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, alpha-amylases obtained from Bacillus , e.g., a special strain of Bacillus licheniformis , described in more detail in GB 1 ,296,839.

Examples of amylases are the a-amylases from Bacillus licheniformis, from Bacillus amyloliquefaciens or from Bacillus stearothermophilus and, in particular, their improved further developments for use in detergents or cleaning agents.

The enzyme from Bacillus licheniformis is available from the Novozymes company under the name Termamyl® and from the Danisco / Genencor company under the name Purastar®ST. Further development products of this a-amylase are available from Novozymes under the trade names Duramyl® and Termamyl®ultra, from Danisco I Genencor under the name Purastar®OxAm and from Daiwa Seiko Inc., Tokyo, Japan, as Keistase®.

The a-amylase from Bacillus amyloliquefaciens is sold by the Novozymes company under the name BAN®, and variants derived from the a-amylase from Bacillus stearothermophilus under the names BSG® and Novamyl®, also from the Novozymes company.

Furthermore, the a-amylase from Bacillus sp.

A 7-7 (DSM 12368) and the cyclodextrin glucanotransferase (CGTase) from Bacillus agaradherens (DSM 9948) should be emphasized.

The amylolytic enzymes disclosed in the international patent applications WO 03/002711 , WO 03/054177 and WO071079938 can also be used.

Fusion products of all the molecules mentioned can also be used. In addition, the further developments of the a-amylase from Aspergillus niger and A. oryzae available from the Novozymes company under the trade names Fungamyl® are suitable. Further commercial products that can be used advantageously are, for example, the Amylase-LT® and Stainzyme® or Stainzyme ultra® or Stainzyme plus®, the latter also from Novozymes. Variants of these enzymes obtainable by point mutations can also be used according to the invention.

Other commercially available amylases are: Amplify Prime EC 110 L, Amplify Prime 100 L, PREFERENZ S 110, Bioamyl P, Stainzyme Plus Evity, Stainzyme® Plus Evity® EC 12 T, Stainzyme® Plus Evity® EC 24 T, Stainzyme® Plus Evity® 24 T, EFFECTENZ™ S 100, EFFECTENZ™ S210, Bialfa T, Achieve Alpha EC 110 L, Achieve Alpha 100 L, Achieve® Advance 150 T, Stainzyme, Amplify, Duramyl, Novamyl,

Amplify™ Prime 100 L is preferably comprised in the pouch according to the instant invention

Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium , e.g., the fungal cellulases produced from Humicola insolens, Myceliophthora thermophila and Fusarium oxysporum disclosed in U.S. Pat. Nos. 4,435,307, 5,648,263, 5,691 ,178, 5,776,757 and WO 89/09259.

Examples of cellulases (endoglucanases, EG) is the fungal, endoglucanase (EG) -rich cellulase preparation or its further developments, which is offered by the Novozymes company under the trade name Celluzyme®.

The products Endolase® and Carezyme®, also available from Novozymes, are based on the 50 kD-EG, or the 43 kD-EG from Humicola insolens DSM 1800. Other commercial products from this company that can be used are Cellusoft®, Renozyme® and Celluclean®. Cellulases, for example, which are available from the company AB Enzymes, Finland, under the trade names Ecostone® and Biotouch®, and which are at least partly based on the 20 kD EG from Melanocarpus, can also be used. Other cellulases from AB Enzymes are Econase® and Ecopulp®. Further suitable cellulases are from Bacillus sp. CBS 670.93 and CBS 669.93, the ones from Bacillus sp. CBS 670.93 from the company Danisco / Genencor is available under the trade name Puradax®. Other commercial products from Danisco / Genencor that can be used are “Genencor detergent cellulase L” and IndiAgeONeutra.

Other commercially available cellulases are: Carezyme® Premium EC 4510 L, Carezyme® Premium Evity® EC 5000 T, Carezyme® Premium 4500 L, Carezyme® Premium Evity® 5000 T, REVILATENZ 200, Biotouch DCL/FCL, Biosoft L Pure, Rocksoft, Retrocell, Retrocell ZircoN, Puradax EG 7000L, Biotouch FCC, BIOCELULASA MC L, BIOCELULASA MC P, Celluclean® EC 5000 L, Celluclean® 5000 L, REVILATENZ 200, Biotouch FLX, Lavergy® C Bright 100 L, Celluclean® Evity® EC 4500 T, Celluclean® Evity® 4500 T, Biotouch DCC,

Further preferred enzymes present in the pouch of the instant invention are those known under the term glycosidases (E.C. 3.2.1.X) . These include in particular arabinases, fucosidases, Galactosidases, galactanases, arabico-galactan-galactosidases, mannanases (also referred to as mannosidases or mannases), glucuronosidases, agarase, carrageenases, pullulanases, B- glucosidases, xyloglucanases (xylanases), xanthanases and pectin-degrading enzymes.

Preferred glycosidases are also summarized under the term hemicellulases. Hemicellulases include in particular mannanases, xyloglucanases (xylanases), B-glucosidases and carrageenases and also pectinases, pullulanases and B-glucanases. Pectinases are pectin-degrading enzymes, the hydrolytic pectin-degrading enzymes in particular belonging to the enzyme classes EC 3.1 .1 .1 1 , EC 3.2.1 .15, EC 3.2.1 .67 and EC 3.2.1 .82. In the context of the present invention, the pectinases also include enzymes with the designations pectin lyase, pectin esterase, pectin demethoxylase, pectin methoxylase, pectin methylesterase, pectase, pectin methylesterase, pectinoesterase, pectin-galle-lactase, pectin-gal-galase, pectin-gal-galase, pectin-gal-galase, pectin-gal-galase, pectin-gal-galase, pectin-gal-galase, pectin-gal-galonase, pectin-galactolase, endopoly-nolase, pectin-poly-galase, pectin-polyhydrolase, pectin-poly-galase, pectinpolyhydrolase, pectin-poly-galase, pectin-poly-galase -a-1 , 4-galacturonide glycanohydrolase, endogalacturonase, endo-D-galacturonase, galacturan 1 , 4-a-galacturonidase, exopolygalacturonase, poly (galacturonate) hydrolase, exo-D-galacturonase, exo-D- galacturonanase -Galacturonase, exo-poly-a-galacturonosidase, Exopolygalacturonosidase or exopolygalacturanosidase.

Examples of enzymes suitable in this regard are, for example, under the names Gamanase®, Pektinex AR® or Pectaway® from Novozymes, under the name Rohapec® B1 L from AB Enzymes and under the name Pyrolase® from Diversa Corp., San Diego, CA, USA.

The B-glucanase obtained from Bacillus subtilis is available under the name Cereflo® from the Novozymes company.

Glycosidases or hemicellulases which are particularly preferred according to the invention are mannanases, which for example, under the trade name Mannaway® by the Novozymes company or Purabrite® by the Danisco I Genencor company.

Examples of commercially available mannanases are: Mannaway® EC 200 L, Mannaway® EC 108 L, Mannaway® 200 L, Mannaway® 100 L, PREFERENZ M100, Biotouch M, Biomananasa 2XL. A mannanase preferably comprised in the pouch according to the instant invention is Mannaway®

4.0 L.

Examples of commercially available pectate lyases are: Xpect® EC 1000 L, Xpect® EC 1000 T, Xpect® 1000 L, Xpect® 1000 T, PREVERENZ F 1000, Pectex Pure, Lavergy® Pro 106 L, Lavergy® Pro 106 LS.

Examples of commercially available licheninases are: Lift INTENT 100 L, Lift INTENT 100 T.

Suitable lipases and cutinases include those of bacterial or fungal origin. Chemically modified or protein engineered mutant enzymes are included. Examples of lipases or cutinases are those originally from Humicola lanuginose (Thermomyces lanuginosus) obtainable or further developed therefrom, in particular those with the amino acid substitution D96L. They are sold, for example, by the company Novozymes under the trade names Lipolase®, Lipolase®Ultra, LipoPrime®, Lipozyme® and Lipex®.

Another lipase which can be used advantageously is available from the Novozymes company under the trade name Lipoclean®.

Furthermore, for example, the cutinases that were originally isolated from Fusarium solani pisi and Humicola insolens can be used. Lipases that can also be used are available from Amano under the names Lipase CE®, Lipase P®, Lipase B®, or Lipase CES®, Lipase AKG®, Bacillis sp. Lipase®, Lipase AP®, Lipase M-AP® and Lipase AML® available. For example, the lipases or cutinases from the Danisco I Genencor company can be used whose starting enzymes were originally isolated from Pseudomonas mendocina and Fusarium solanii. Other important commercial products are the preparations M1 Lipase® and Lipomax® originally sold by the company Gist- Brocades (now Danisco I Genencor) and those from the company Meito Sangyo KK, Japan, under the name Lipase MY-30®, Lipase OF® and Lipase PL®, as well as the product Lumafast® from Danisco I Genencor.

Other example of commercially available lipases are: Lipex® Evity® EC 100 L, Lipex® Evity® EC 100 T, Lipex® Evity® EC 200 L, Lipex® Evity® 100 L, Lipex® Evity® 100 T, Lipex® Evity® 200 L, PREFERENZ L 100, Biolipasa 2XL, Biolipasa L, Biolipasa P, Lipoclean, Lipolase, Lipolase Ultra. A lipase preferably comprised in the pouch according to the instant invention is Lipex™ 100 L Evity.

Examples of commercially available Phosphodiesterase are: Pristine from Novozyme

Suitable peroxidases/oxidases include those of plant, bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Examples of useful peroxidases include peroxidases from Coprinus , e.g., from C. cinereus , and variants thereof as those described in WO 93/24618, WO 95/10602, and WO 98/15257.

Commercially available peroxidases include Guardzyme™ (Novozymes A/S). The pouch according to the instant invention can further comprise one or more auxiliary agents selected from the group consisting of bleaching systems, hydrotropes, polymers, which may be synthetic, biopolymers, anti-redeposition aids, fiber protection agents, soil release agents, dye transfer inhibitors, fabric hueing agents, opacifiers, blueing dyes, enzyme stabilizing agents, solvents, viscosity modifiers, preservatives, pH-regulators and salts like NaCI and Na2SC>4. Preferably said auxiliary agent is comprised in the liquid composition comprising the at least one biosurfactant.

The instant invention further provides the use of a pouch according to the instant invention for cleaning a surface of an article, for example in automatic dish washing or laundry applications, preferably a textile or a fabric.

Preferably the use according to the instant invention is characterized in that the surface of the article is cleaned from fat and/or oil, preferably from solid fatty stains.

The use according to the instant invention preferably uses the preferred biosurfactants and polyvinyl alcohols as described above to be preferably used in the pouches of the instant invention.

The examples adduced hereinafter describe the present invention by way of example, without any intention that the invention, the scope of application of which is apparent from the entirety of the description and the claims, be restricted to the embodiments specified in the examples.

Examples:

Example 1: Integrity and solution time measured upon contact with water.

Preparation of liquid detergent formulations:

Exemplary formulations described below were prepared according to the following protocol: Initially, a measured amount of water was introduced into a glass beaker of a suitable size. Subsequently, ingredients 1 - 5 were added at room temperature and under vigorous stirring. All formulations contained ingredients 2 - 5, while ingredients 1a, 1 b, and 1 c, were added only to test formulation 1 , Benchmark formulation 1 , and Benchmark formulation 2, respectively. The ingredients were added in no specific or uniform sequence as the order of addition to the solution was not critical. Subsequently, any remaining amount of water was introduced to ensure desired concentration of ingredients. Finally, ingredient 6 (monoethanolamine) was added in the solution and the mixture was stirred vigorously for 1 h at temperature between 60 and 65°C. In the next step, the mixture was cooled down to 35°C and Ingredient 7 (citric acid) was added under continuous stirring until pH of formulation was adjusted to 8.0. Subsequently, ingredient 8 (Enzymes) were introduced in the formulation and the mixture was stirred for 5 minutes to ensure a homogenous solution. The exemplary compositions were easily pourable and stable at room temperature for extended period.

The above-mentioned method was utilized to prepare three formulation, Test formulation 1 , Benchmark formulation 1 and Benchmark formulation 2. All of them are composed of analogous ingredients at the same concentration and differ with the type of anionic surfactant used, so Dirhamnolipid, sodium salt, Linear alkylbenzene sulfonate, sodium salt, and Sodium laureth sulfate in Test formulation 1 , Benchmark formulation 1 and Benchmark formulation 2, respectively. It is worth noting that Linear alkylbenzene sulfonate, sodium salt, and Sodium laureth sulfate are standard surfactants routinely used in detergent formulations of water-soluble pouches.

Rhamnolipids used were prepared as described in example 1 of EP3061442 and resemble dirhamnolipids; mono-rhamnolipids were prepared as in example 2 of EP3061442. Compositions of Benchmark formulation 1 , Test formulation 1 , and Test formulation 2 evaluated for their compatibility with water-soluble films:

Preparation of detergent pouches:

Detergent pouches were prepared using water-soluble PVOH foil. Initially, two pieces of film were heat-sealed from three sides using a manual-sealing device to form a pouch of 6 cm x 10 cm in size. Created pouch was subsequently filled with 25 g of liquid detergent solution (Test formulation 1 , Benchmark formulation 1 or Benchmark formulation 2) and closed with a heat-sealing device. External edges outside the sealing were removed by cutting to form the final pouch.

Solubility test of water-soluble pods:

Prior to the water solubility test, pouches filled with Test formulationl , Benchmark formulation 1 , and Benchmark formulation 2, respectively, were stored at room temperature and at ambient humidity for 48 hours. This allowed sufficient conditioning time for the liquid detergent content to affect properties of water-soluble film.

Test was performed according to the following protocol:

For the test, 1 L of water was placed in a suitable glass container and conditioned to 40°C. A pouch containing liquid detergent was attached by its corner to a metal holder and allowed to hand freely. Subsequently, the pouch was immersed in water. It was ensured that the pouch was hanging vertically without touching the bottom of glass container. No stirring was applied during the measurement. According to the described test, time between immersing of the pouch in water and the first leakage of detergent through was measured. Additionally, time required for the pouch immersed in water to lose its physical integrity and fall from the metal holder on the bottom of the glass container was also recorded. The average results recorded for pouches containing each of the formulations are presented below.

Results of test examining impact of liquid detergents on water-soluble films:

As summarized above water-soluble pouches containing Test formulation 1 surprisingly exhibit improved integrity after immersing them in water than pouches containing Benchmark formulation 1 and Benchmark formulation 2. Therefore, it was surprisingly discovered that rhamnolipid-containing liquid detergents have positive effect on integrity of water-soluble pouches by making them less susceptible to premature opening upon contact with water.

Example 2: Liquid formulation free of monoethanolamine

Preparation of liquid detergent formulations:

Exemplary formulations described below were prepared according to the following protocol: Initially, a measured amount of water was introduced into a glass beaker of a suitable size. Subsequently, ingredients 1 - 5 were added at room temperature and under vigorous stirring. All formulations contained ingredients 2 - 5, while ingredients 1a, 1 b, and 1 c, were added only to test formulation 2, Benchmark formulation 3, and Benchmark formulation 4, respectively. The ingredients were added in no specific or uniform sequence as the order of addition to the solution was not critical. Subsequently, any remaining amount of water was introduced to ensure desired concentration of ingredients. Finally, ingredient 6 (sodium hydroxide) was added in the solution and the mixture was stirred vigorously for 1 h at temperature between 60 and 65°C. In the next step, the mixture was cooled down to 35°C and Ingredient 7 (citric acid) was added under continuous stirring until pH of formulation was adjusted to 8.0. Subsequently, ingredient 8 (Enzymes) were introduced in the formulation and the mixture was stirred for 5 minutes to ensure a homogenous solution. The exemplary compositions were easily pourable and stable at room temperature for extended period. The above-mentioned method was utilized to prepare three formulation, Test formulation 2, Benchmark formulation 3 and Benchmark formulation 4. All of them are composed of analogous ingredients at the same concentration and differ with the type of anionic surfactant used, so Dirhamnolipid, sodium salt, Linear alkylbenzene sulfonate, sodium salt, and Sodium laureth sulfate in Test formulation 2, Benchmark formulation 3 and Benchmark formulation 4, respectively.

Compositions of Benchmark formulation 2, Test formulation 3, and Test formulation 4 evaluated for their properties:

It was surprisingly discovered that after cooling down all three formulations (Test formulation 2, Benchmark formulation 3, and Benchmark formulation 4), only Test formulation 2 maintained its liquid character while Benchmark formulation 3, and Benchmark formulation 4 solidified. Therefore, Test formulation 2 can be processed at room temperature and maintains its liquid character after being placed inside the water-soluble pouch, Benchmark formulation 3 and Benchmark formulation 4 solidify at room temperature therefore hindering their usage as liquid detergent for water-soluble pouches. Therefore, it was surprisingly discovered that biosurfactants allow for formulating concentrated liquid detergents for water-soluble pouches without any addition of monoethanolamine.

Example 3: Dye stability during storage

The formulations are packed into pouches according to the method in example 1 . The pouches are stored without covering on the lab bench over a period of 3 months.

Pouches containing Benchmark formulation 5 and Benchmark formulation 6 apparently bear a paler colour compared to the pouches containing Test formulation 3. The rhamnolipid and the colorant, acid blue 3, comprising pouches exhibit a better brilliance of the color compared to the others.

Further example of formulations:

Further exemplary liquid detergent compositions containing rhamnolipids were prepared according to the formulations described in the table below. All exemplary formulations were subsequently used to obtain water-soluble detergent pouches. For this reason, two pieces of water-soluble PVOH film were heat-sealed from three sides using a manual-sealing device to form a pouch of 6 cm x 10 cm in size. The created pouch was subsequently filled with 25 g of liquid detergent solution (Formulation 1 - 26) and closed with a heat-sealing device. External edges outside the sealing were removed by cutting to form the final pouch. Sophorolipid used is a sophorolipid REWOFERM SL ONE from Evonik, which has a lactone to acid ratio of 40:60.

Glucolipids were produced according to example 2 of WO2019154970 via fermentation

Claims

1 . A detergent compartment pouch having at least one water-soluble film forming at least one compartment, wherein the at least one compartment contains a liquid composition, said liquid composition comprises at least one biosurfactant, characterized in that said water-soluble film comprises polyvinyl alcohol.

2. Pouch according to claim 1 , characterized in that said biosurfactant is selected from rhamnolipids, glucolipids and sophorolipids, preferably rhamnolipids and glucolipids, most preferably rhamnolipids.

3. Pouch according to claim 1 or 2, characterized in that said biosurfactant is selected from rhamnolipids, comprising either 51 wt.-% to 100 wt.-%, preferably 60 wt.-% to 95 wt.-%, particularly preferably 80 wt.- % to 90 wt.-%, of mono-rhamnolipids, or 71 wt.-% to 100 wt.-%, preferably 75 wt.-% to 95 wt.-%, particularly preferably 80 wt.-% to 90 wt.-%, of di-rhamnolipids where the percentages by weight refer to the sum of all of the rhamnolipids comprised in the liquid composition.

4. Pouch according to any of the preceding claims, characterized in that the biosurfactant is comprised in said liquid composition in an amount of 0.1 wt.-% to 50 wt.-%, preferably 1.0 wt.-% to 20 wt.-%, more preferably 5.0 wt.-% to 15 wt.-%, where the percentages by weight refer to the total liquid composition.

5. Pouch according to any of the preceding claims, characterized in that it comprises in said liquid composition at least one non-biosurfactant, preferably selected from the group of anionic, cationic, non-ionic, semi-polar, amphoteric and zwitterionic surfactants.

6. Pouch according to claim 5, characterized in that the biosurfactant is comprised in said liquid composition in an amount of 50 wt.-% to 100 wt.-%, preferably 60 wt.-% to 98 wt.-%, more preferably 80 wt.-% to 95 wt.-%, where the percentages by weight refer to the sum of all surfactants comprised in the liquid composition.

7. Pouch according to any of the preceding claims, characterized in that said liquid composition comprises at least one water soluble dye.

8. Pouch according to claim 7, characterized in that said water soluble dye is selected from the group comprising acid blue 1 , acid blue 3, acid blue 7, acid blue 145, acid blue 182, acid blue 185, acid blue 193, C.l. acid blue 9, C.l. acid blue 93, C.l. pigment blue 29, C.l. solvent blue 3, and C.l. reactive blue 116. Pouch according to any of the preceding claims, characterized in that said liquid composition comprises water in an amount of 0.1 wt.-% to 25 wt.-%, preferably 8.0 wt.-% to 20 wt.-%, more preferably 16 wt.-% to 19 wt.-%, where the percentages by weight refer to the total liquid composition. Pouch according to any of the preceding claims, characterized in that said liquid composition comprises monoethanolamine (MEA) in an amount of less than 15 wt.-%, preferably less than 10 wt.-%, more preferably 0.01 wt.-% to 4.0 wt.-%, where the percentages by weight refer to the total liquid composition. Pouch according to any of the preceding claims, characterized in that said polyvinyl alcohol has a degree of hydrolysis of 70 mol %to 100 mol %, preferably 80 mol % to 90 mol %, particularly preferably 81 mol %to 89 mol %, and in particular 82 mol % to 88 mol %. Pouch according to any of the preceding claims, characterized in that said polyvinyl alcohol has a molecular weight in the range from 10,000 g/mol to 100,000 g/mol, preferably from

1 ,000 g/mol to 90,000 g/mol, particularly preferably from 12,000 g/mol to 80,000 g/mol and in particular from 14,000 g/mol to 68,000 g/mol. Pouch according to any of the preceding claims, characterized in that said water-soluble film comprises said polyvinyl alcohol in an amount of at least 50 wt.-%, preferably of at least 70 wt.-%, particularly preferably of at least 80 wt.-% and in particular of at least 92 wt.-%, where the percentages by weight refer to the total weight of the water-soluble film. Use of a pouch according to any of the preceding claims for cleaning a surface of an article, preferably a textile or a fabric.