KR20220151851A - Functional cosmetic composition comprising complex extract from ecklonia cava, sea swallow's nest, kelp and grape as effective ingredient, and manufacturing method thereof - Google Patents

Abstract

A complex extract of Ecklonia cava, nests of Aerodramus fuciphagus, kelp and grape in a specific blending ratio presented in the present invention has an anti-inflammatory effect and also, has skin elasticity improvement or wrinkle reduction effects, and thus can be usefully used in the manufacture of functional cosmetics. An objective of the present invention is to provide a cosmetic composition exhibiting anti-inflammatory efficacy by inhibiting NO production.

Description

Functional cosmetic composition for mitigating skin inflammation caused by fine dust containing complex extracts of Ecklonia cava, bird's nest, kelp and grape as active ingredients and its manufacturing method EFFECTIVE INGREDIENT, AND MANUFACTURING METHOD THEREOF}

The present invention relates to a functional cosmetic composition comprising complex extracts of Ecklonia cava, bird's nest, kelp and grapes, and more specifically, to a functional cosmetic composition that can help improve skin inflammation caused by fine dust.

Particulate matter floating in the air is called dust, and among them, particles of a size that cannot be discerned with the naked eye are called fine dust. The smaller the particle size of the fine dust, the greater the harmful effect on the human body and the environment.

Ultrafine dust containing various chemicals such as heavy metals weakens the metabolic flow of the skin, lowers the sebum control function, and causes dryness, itching, and skin troubles. Harmful substances such as heavy metals and environmental hormones contained in fine dust can induce allergic reactions by promoting the secretion of inflammatory cytokines in cells and increasing the number of white blood cells.

In addition, when fine dust is absorbed into the skin, it stimulates pigment cells to create wrinkles and age spots, and when absorbed into the body, it creates active oxygen, which damages collagen in the skin and reduces skin elasticity, resulting in extrinsic aging. can

Extrinsic aging is caused by the external environment, unlike natural aging that occurs with aging. Currently, many studies are being conducted in the industry to suppress aging by controlling environmental factors such as ultraviolet rays, oxidation, and drying.

These environmental factors mainly cause wrinkles by destroying or denaturing collagen proteins, elastin proteins, etc. contained in the dermal layer. In particular, ultraviolet rays oxidize skin cells (fibroblasts) and cause reduction and destruction of elastin production. will bring

NO is known to have various functions such as blood pressure regulation, platelet aggregation, and signal transmission in vivo. A pro-inflammatory cytokine or LPS acts on immune cells to increase the production of NO by the action of inducible nitric oxide synthase (iNOS) from L-arginine. As excessive NO production is known to be related to chronic inflammation, many studies are being conducted on the development of selective NO biosynthesis inhibitors to control chronic inflammation through the regulation of iNOS activity (Sharma et al., 2007; Korhonen et al. , 2005).

On the other hand, as consumers' interest in side effects, skin irritation, and damage caused by the use of cosmetics containing a large amount of chemical components increases, attempts to develop cosmetics using natural materials such as various plants are increasing. To this end, it is required to search for natural substances that can be safely used in the human body while maintaining various functions such as relieving inflammation, preventing oxidation, or enhancing skin elasticity. Fruits or algae may be raw material candidates that can meet these requirements because they contain various functional substances useful for physiological activity, but have low cytotoxicity.

An object of the present invention is to provide a cosmetic composition exhibiting anti-inflammatory efficacy by inhibiting NO production.

Another object of the present invention is to provide a cosmetic composition exhibiting skin elasticity or wrinkle improvement efficacy.

Another object of the present invention is to provide a cosmetic composition with no cytotoxicity and less skin irritation.

Another object of the present invention is to provide a method for preparing the cosmetic composition.

According to one aspect of the present invention, it is possible to provide a cosmetic composition having an anti-inflammatory effect, containing complex extracts of Ecklonia cava, bird's nest, kelp, and grapes as active ingredients.

The cosmetic composition further has an effect of improving skin elasticity or wrinkles.

The compounding ratio of each raw material extract in the composite extract may be 3 to 5: 3 to 5: 1: 0.5 to 2 in the order of Ecklonia cava, bird's nest, kelp and grapes.

The composite extract may be contained in an amount of 0.1% to 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be, but is not limited to, formulations of lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray or cleanser.

In addition, according to another aspect of the present invention, a method for preparing a cosmetic composition having an anti-inflammatory effect containing the above-described composite extract of Ecklonia cava, bird's nest, kelp, and grape as an active ingredient can be provided.

The method may include preparing a composite extract by mixing Ecklonia cava extract, bird's nest extract, kelp extract, and grape extract in the above mixing ratio, or mixing each material in the above mixing ratio and then extracting.

The extraction method involves cutting or pulverizing Ecklonia cava, bird's nest, kelp, and grapes, adding 1 to 15 weight multiples of a solvent, and then ultrasonicating at a frequency of 20 to 40 kHz for 30 minutes to 3 hours at a temperature range of 15 to 25 ° C. This may include extraction.

The manufacturing method of the cosmetic composition of the present invention is to add 0.1% to 30% by weight of the complex extract to a general cosmetic preparation composition, and then toner, serum, essence, lotion, cream, powder, foundation, pack according to a conventional method , It may include a step of formulating into a formulation such as a patch, mask sheet, gel ointment, spray or cleanser.

The cosmetic composition containing the complex extracts of Ecklonia cava, bird's nest, kelp and grapes as an active ingredient has excellent anti-inflammatory effect and further possesses skin improvement effects such as skin elasticity or wrinkle improvement.

The cosmetic composition of the present invention contains complex extracts of Ecklonia cava, bird's nest, kelp and grapes as active ingredients, and exhibits anti-inflammatory effects.

Ecklonia cava is a seaweed belonging to the seaweed family of brown algae, kelp, and is mainly inhabited and distributed in the southern coast of Korea, the coast of Jeju, and the coast of Japan. It feeds on abalone and conch, and is used as a major raw material for making alginic acid and potassium iodide.

Ecklonia cava has recently emerged as a functional material as its various physiological activities have been revealed through active research on marine biological resources. The existence of several phlorotannins has been isolated and reported in Ecklonia cava and Gompi. Phlorotannin is a polyphenolic compound with phloroglucinol as a basic unit. Phlorotannin of seaweeds such as Ecklonia cava has been reported to have thrombogenesis inhibitory activity, antioxidant activity, and cardiovascular protective effect.

The sea swallow's nest is made by mixing seaweed and fish bones and saliva, and is known to be rich in nutrients such as amino acids, sugars, vitamins and minerals, and colloidal protein, and have a great tonic effect. .

The slime that comes out of the swallow's mouth turns seaweed into a gelatin-like colloid, which is collected and hardened into a swallow's nest. The mucin component of the bird's nest is composed of 17 amino acids.

Kelp is a 2-3 year old seaweed belonging to the brown algae family and is rich in carbohydrates and minerals. It contains 16% of moisture, 7% of protein, 1.5% of fat, 49% of carbohydrates, and 26.5% of minerals, and 5-10% of carbohydrates are cellulose, and the rest are polysaccharides such as laminarin and fucoidin based on alginic acid. These water-soluble polysaccharides, as dietary fibers, have effects such as intestinal regulation, discharge of heavy metals, prevention of cholesterol deposition in blood vessels, and anti-aging.

Grapes are a fruit that is high in glucose and fructose, rich in potassium and iron, and also contains vitamins. Grape seeds contain 40 times more antioxidants than vitamin E. Proanthocyanidin, an astringent component present in grape seeds, and resveratrol in grape skins are a type of polyphenol, and resveratrol is known to have anticancer and antioxidant activities, and to lower serum cholesterol.

As used herein, the term “anti-inflammatory” is used to include the amelioration or reduction, inhibition or delay of an inflammatory response. These inflammatory responses include activation of various inflammatory mediators and enzymes related to immune cells (e.g., iNOS, COX-2, etc.), secretion of inflammatory mediators (e.g., secretion of NO, TNF-α, IL-6, etc.), body fluid infiltration, It is accompanied by a series of complex physiological reactions such as cell migration and tissue destruction, and is externally manifested by symptoms such as erythema, pain, edema, and fever.

The cosmetic composition of the present invention further possesses an anti-inflammatory effect through suppression of NO production, TNF-α, and IL-6 expression, as well as an effect of improving skin elasticity or wrinkles through inhibition of elastase.

In the present invention, the extracts of Ecklonia cava, bird's nest, kelp, and grapes are not particularly limited, but may be specifically used in various forms, such as an extract, a powder obtained by drying the extract, and a concentrate obtained by concentrating and filtering the extract.

The extract may be in the form of, for example, tincture containing alcohol, purified water, oil, etc. as a leaching solvent, concentrate, extract, fluid extract, and the like.

In the present invention, the blending weight ratio of Ecklonia cava, bird's nest, kelp, and grape extracts may be 3 to 5: 3 to 5: 1: 0.5 to 2 in order, and the desired functionality can be realized within the range of the blending ratio.

In one embodiment, the blending ratio of the Ecklonia cava, bird's nest, kelp, and grape extracts may be measured based on the solid weight of the extract. At this time, the solid weight of the extract may be a solid obtained by freeze-drying after extracting by adding a solvent to pieces or pulverized materials of Ecklonia cava, bird's nest, kelp, and grapes, or a mixture thereof. In one embodiment, the grapes may be raw, dry kernels excluding skins, dry skins, or a mixture thereof.

In another embodiment, the mixing ratio of the Ecklonia cava, bird's nest, kelp, and grape extracts may be set based on the weight of the raw material. In this case, the weight of the raw material may be the weight of each raw material or a mixture thereof before adding the extraction solvent.

The composite extract may be contained in an amount of 0.1% to 30% by weight, specifically 1% to 25% by weight based on the total weight of the cosmetic composition. Expected functionality can be expressed within the above range.

The cosmetic composition may be any one formulation of lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray, and cleanser, but is not limited thereto.

The cosmetic composition of the present invention may further include a composition for preparing a cosmetic together with the composite extract of Ecklonia cava, bird's nest, kelp and grape.

The cosmetic preparation composition is intended to implement a cosmetic effect according to the purpose and formulation of the cosmetic along with the composite extract, and is not particularly limited as long as it is a component used as a general cosmetic subject and excipient.

Specifically, the composition for preparing a cosmetic composition includes a solvent, an oil and fat component, a humectant, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, a UV absorber, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, and a plant oil. , It may include one or more kinds of pigments, flavors, and the like.

For example, the composition for preparing the cosmetic is purified water (water), alcohol (alcohol), glycerin (glycerin), myristic acid (myristic acid), butylene glycol, potassium hydroxide (potassium hydroxide) , polyethylene glycol, stearate, stearic acid, behenyl alcohol, microcrystalline wax, phenoxyethanol, ethylhexylglycerin ( ethylhexylglycerin), tocopheryl acetate, 1,2-hexanediol, caprylyl glycol, hydroxyethylene cellulose, hydroxy ethyl cellulose ethyl cellulose), xanthan gum, collagen, sodium citrate, citric acid, magnesium ascorbate, allantoin, panthenol, betaine ( betaine, Cetearyl Alcohol, Sunflower oil, Cetearyl Olivate, Sorbitan Olivate, Glyceryl Stearate and fragrance ) may include one or more of

Another aspect of the present invention relates to a method for producing a functional cosmetic composition containing the above-described composite extract of Ecklonia cava, bird's nest, kelp and grape as an active ingredient. The method includes mixing extracts of Ecklonia cava, bird's nest, kelp, and grapes, or mixing Ecklonia cava, bird's nest, kelp, and grapes, and then extracting with a solvent to prepare a composite extract.

In one embodiment, in the process of preparing a composite extract of Ecklonia cava, bird's nest, kelp, and grapes, dried Ecklonia cava, bird's nest, kelp, and grapes may be pulverized or shredded, and then a solvent may be added.

The solvent is not particularly limited as long as it can be used as an extraction solvent for preparing cosmetics, and specifically may be water, an organic solvent, or a mixture thereof.

The organic solvent may be an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms such as ethanol, propanol, methanol, and n-butanol; polar organic solvents such as acetone and 1,3-butylene glycol; non-polar organic solvents such as ether, hexane, and ethyl acetate; Vegetable organic solvents such as soybean oil and sesame oil may be included.

The organic solvent may be used alone or in combination of two or more. In the case of using a mixed solvent, the mixing composition and ratio can be appropriately adjusted according to the purpose.

Specifically, the complex extract of the present invention can be prepared by ultrasonic extraction using 5 to 30% of butylene glycol.

That is, it may be prepared by adding an extraction solvent of 1 to 15 weight multiples of each raw material and subjecting it to low-temperature ultrasonic treatment at a frequency of 20 to 40 kHz for 30 minutes to 3 hours at 15 to 25 ° C. Specifically, the ultrasonic treatment may be performed at a temperature of 17 to 22 ° C. for 30 minutes to 2 hours at a frequency of 22 to 38 kHz, preferably at a temperature of 20 ° C. for 1 hour at a frequency of 25 to 27 kHz. can be done In addition, the ultrasonic treatment may be repeated 1 to 5 times.

When treated under the above conditions, it is possible to shorten the extraction time, increase the extraction yield, and minimize the possibility of denaturation and destruction of active ingredients exhibiting physiological activity.

In addition to the ultrasonic extraction method, extraction methods known in the art such as bath extraction, reflux cooling extraction, cold extraction, and percolation may also be used.

The extract of the present invention may include not only an extract material extracted by the above-described extraction solvent, but also an extract material extracted through a conventional purification process. Active fractions obtained through various additional purification methods, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, may also be included in the extract of the present invention.

The composite extract of Ecklonia cava, bird's nest, kelp and grapes of the present invention can be used not only for cosmetic compositions, but also for the preparation of functional foods that help relieve inflammation or improve skin elasticity or wrinkles. In this case, the mixing ratio of each raw material and the extraction process are the same as in the previous technique. Examples of the extraction solvent include water, ethanol, and mixtures thereof, and the range of the solvent is well known to those skilled in the art.

In one embodiment, the preparation of the composite extract may be to prepare extracts of Ecklonia cava, bird's nest, kelp, and grapes, respectively, and then mix them in a weight ratio of 3 to 5: 3 to 5: 1: 0.5 to 2.

In another embodiment, preparation of the complex extract may be performed by mixing Ecklonia cava, bird's nest, kelp, and grapes in a weight ratio of 3 to 5: 3 to 5: 1: 0.5 to 2, and then adding an extraction solvent.

The manufacturing method of the cosmetic composition of the present invention is to add 0.1% to 40% by weight of the composite extract to a general cosmetic preparation composition, and then toner, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet , The step of formulating into any one formulation of a gel ointment, spray, and detergent may be further included.

The formulation method is not particularly limited, and may be obtained through various methods known in the art to which the present invention pertains.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this is presented as a preferred example of the present invention and cannot be construed as limiting the present invention by this for any reason.

Contents not described herein will be omitted because those skilled in the art can sufficiently technically infer them.

Example 1: Preparation of complex extract 1 (weight ratio of Ecklonia cava, bird's nest, kelp and grape 1: 1: 1: 1)

The dried Ecklonia cava (Jeju) was rinsed in water to remove salt, dried, and cut into about 2 cm in size. A bird's nest (made in Malaysia) was obtained, dried, and cut to a size of about 2 cm. Kelp (Jeju) was rinsed in water to remove salt, dried, and cut into about 2 cm in size. Grapes were purchased and used by drying the peeled kernels (made in the United States).

100 g of Ecklonia cava prepared as described above, 100 g of bird's nest, 100 g of kelp, and 100 g of grapes were added with 10 times 30% butylene glycol based on the total weight of the mixture, and a sonicator (POWERSONIC 510, Hwashin) was prepared for 1 hour at a temperature of 20 ° C. TECH) using ultrasonic treatment at a frequency of 25-27 kHz, leaving it in an oven (ON-22GW, JEIO TECH) for 2 hours at a temperature of 85 ° C, and then filtering the extract with a 0.2 μm filter to prepare an extract did

Example 2: Preparation of complex extract 2 (Ecklonia cava, bird's nest, kelp and grape weight ratio (2: 2: 1: 1)

200 g of Ecklonia cava prepared as in Example 1, 200 g of bird's nest, 100 g of kelp, and 100 g of grapes were added with 10 times 30% butylene glycol based on the total weight of the mixture, and the sonicator (POWERSONIC 510, Hwashin Tech), ultrasonic treatment at a frequency of 25 ~ 27 kHz, and left in an oven (ON-22GW, JEIO TECH) for 2 hours at a temperature of 85 ° C. Then, the extract was filtered through a 0.2 μm filter to obtain an extract. was manufactured.

Example 3: Preparation of composite extract 3 (weight ratio of Ecklonia cava, bird's nest, kelp and grape 4: 4: 1: 1)

400 g of Ecklonia cava prepared as in Example 1, 400 g of seabird's nest, 100 g of kelp, and 100 g of grapes were added with 10 times 30% butylene glycol based on the total weight of the mixture, and the sonicator (POWERSONIC 510, Hwashin Tech) to ultrasonic treatment at a frequency of 25 to 27 kHz, and left in an oven (ON-22GW, JEIO TECH) for 2 hours at a temperature of 85 ° C. Then, the extract was filtered with a 0.2 μm filter An extract was prepared.

Comparative Example: Preparation of each single extract

100 g of Ecklonia cava prepared as in Example 1, 100 g of sea bird's nest, 100 g of kelp, and 100 g of grapes were added with 10-fold of 30% butylene glycol based on the weight of each, and stirred in a sonicator (POWERSONIC) for 1 hour at a temperature of 20 ° C. 510, Hwashin Tech) to ultrasonic treatment at a frequency of 25 to 27 kHz, and left in an oven (ON-22GW, JEIO TECH) for 2 hours at a temperature of 85 ° C. Then, the extract was filtered with a 0.2 μm filter A single extract of each was prepared.

Test Example 1: Evaluation of anti-inflammatory activity

In order to evaluate the anti-inflammatory effect of the extracts of Examples and Comparative Examples, nitric oxide (NO) production inhibitory activity was measured.

The amount of NO was confirmed by measuring the amount of nitrite, a stable oxidation product. Specifically, after culturing RAW 264.7 cells (1x10 ⁴ cells/well) in a 96-well plate for 12-18 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium, 1 μg/ml of LPS and extracts were added in a serum-free state. It was administered by % (v / v) concentration and cultured for 24 hours, and 100 μl of cell culture supernatant was mixed with 100 μl of grease reagent (Sigma-Aldrich Co., St. Louis, MO, USA) in a new 96-well plate. Then, absorbance was measured at 550 nm. Nitrite concentration was determined by comparison to a sodium nitrite standard curve. As a positive control, ascorbic acid (100 ppm) was used.

The results are shown in Table 1 and Table 2 below.

[Table 1]

[Table 2]

As shown in the table above, it was found that the composite extract of Example 3 possesses a strong anti-inflammatory activity compared to Comparative Examples or other Examples.

Test Example 2: Evaluation of skin elasticity or wrinkle improvement

In order to evaluate the effect of improving skin elasticity or wrinkles, the elastase inhibitory activity of the extracts of Examples and Comparative Examples was measured.

First, 20 µl of 0.2M Tris-HCl (pH 8.0) containing 0.1% triton X-100 was added to HDF-N cells (5x10 ³ cells/well), which are dermal fibroblasts, and then homogenized using a sonicator. The supernatant obtained by centrifugation at 3,000 rpm for 20 minutes was used as a sample for measuring elastase inhibitory activity. After mixing 60 μl of the 200 μg/ml sample, 100 μl of 0.2M Tris-HCl buffer, and 20 μl of extracts of Examples and Comparative Examples at each concentration % (v/v), 20 μl of 50 mM N-STANA was mixed. After incubation at 37 ° C., absorbance was measured at 405 nm. As a positive control, epigallocatechin gallate (EGCG) (100 ppm) was used. The results are shown in Tables 3 and 4.

[Table 3]

[Table 4]

As shown in the table above, it can be seen that the composite extracts of Examples are generally superior in elastase inhibitory activity compared to Comparative Examples.

Test Example 3: Toxicity evaluation test

The cytotoxicity test was performed by the MTT assay method by adding the composite extracts of the examples during culture of normal human fibroblasts.

Specifically, 1 x 10 ⁴ fibroblasts (HS86) were inoculated in DMEM (Dulbecco's Modified Eagle's Medium) medium supplemented with 10% bovine serum, cultured at 5% CO ₂ and 37°C for one day, and then extract samples were collected at each concentration. % (v/v) was treated with 20 µl each.

After culturing for 1 day, 20 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent was treated and further incubated for 4 hours. Chromosomes were dissolved in dimethyl sulfoxide, and absorbance was measured at 540 nm for relative comparison. As a control, a culture solution not treated with extract samples was used.

Cell viability (%) = 100 - (absorbance of each extract sample) / (absorbance of control group) * 100 Calculate the viability of the cells, and the cell proliferation rate (%) of the control group that is not treated with the extract sample The relative cell proliferation effect when set to 100 is shown in Table 5 below.

[Table 5]

As a result, it was confirmed that the extracts of Examples 1 and 3 did not inhibit cell viability and thus had no cytotoxicity.

Test Example 4: Evaluation of inflammatory factor expression inhibitory activity

In order to additionally verify the anti-inflammatory activity of the complex extract, it was added to culture of normal human fibroblasts, and the ability to inhibit the expression of TNF-α and IL-6, which are inflammatory factors, was evaluated. Specifically, fibroblasts (HS86) were inoculated in a 48-well plate at 4 Х 10 ⁴ cells/well and cultured for 24 hours. Then, after replacing the medium and treating with LPS, 20 μl of the composite extracts of Examples 1 and 3 were added at each concentration % (v / v), and then cultured for 48 hours. As a positive control group, dexamethasone was treated at the same concentration. Compared to the culture medium without any treatment, the relative values of TNF-α and IL-6 concentrations of the culture medium treated with the composite extract were calculated. The concentrations of TNF-α and IL-6 were measured by ELISA assay. Results for the inhibition rate (%) of TNF-α and IL-6 are shown in Table 6 and Table 7, respectively.

[Table 6]

[Table 7]

From the above results, it can be seen that the complex extract of Example 3 significantly suppressed the expression of the inflammatory factors TNF-α and IL-6 compared to Example 1. In particular, at a concentration of 0.5% or more, the expression inhibition rate was higher than that of dexamethasone, a positive control group. Since this result is consistent with the results of the NO inhibitory activity evaluation of Test Example 1, it can be confirmed that the composite extract of Example 3 has particularly excellent anti-inflammatory efficacy.

From Test Examples 1 and 4, the composite extract of Example 3 inhibits NO production and significantly reduces the expression of inflammatory factors TNF-α and IL-6, exerts a strong anti-inflammatory effect, and exhibits high elastase inhibitory activity. It was confirmed that skin elasticity can be increased and skin wrinkles can be improved by having .

Comparison of the anti-inflammatory activities between single extracts and complex extracts of Ecklonia cava, bird's nest, kelp, and grapes showed that the above effects were due to synergistic effects according to the optimal mixing ratio of raw materials.

Therefore, the composite extract of the present invention can be widely used in cosmetics, foods, and pharmaceuticals for the prevention, improvement, or treatment of inflammatory diseases including skin inflammation.

The following preparation examples are only intended to illustrate the implementation of the idea of the present invention and should not be construed as limiting the scope of the present invention thereby.

Preparation Example 1: Preparation of a cream formulation containing complex extracts

A moisturizing cream containing the complex extract (complex) of Example 3 was prepared in a conventional manner according to the composition and composition ratio (weight basis) shown in Table 8 below.

[Table 8]

Preparation Example 2: Preparation of Serum Formulation Containing Composite Extract

Nutrient serums each containing the complex extract (complex) of Example 3 were prepared in a conventional manner according to the compositions and composition ratios (by weight) shown in Table 9 below.

[Table 9]

Comparative Preparation Example 1

A moisturizing cream was prepared in the same manner as in Preparation Example 1, except that the composite extract of Example 1 was used as the composite of Table 8.

Comparative Preparation Example 2

A nutritional serum was prepared in the same manner as in Preparation Example 2, except that the composite extract of Example 1 was used as the composite of Table 9.

Evaluation of moisturizing effect and improvement of skin condition

In order to evaluate the effect of improving the moisturizing feeling and skin condition of the cosmetic composition containing the complex extract of the present invention, sensory evaluation was performed on the moisturizing cream and nutrient serum prepared in Preparation Example.

40 women between the ages of 25 and 35 were divided into two groups, group 1 received moisturizing cream and nourishing serum according to Preparation Examples 1 and 2, and group 2 received moisturizing cream and nourishing serum according to Comparative Preparation Examples 1 and 2 for 1 day. It was continuously used for 6 weeks at a time. Sensory evaluation was very good (5 points), good (4 points), average (3 points), bad (2 points), very bad ( 1 point), and the results are shown in Tables 10 and 11, respectively.

[Table 10]

[Table 11]

As shown in Tables 10 and 11, compared to Comparative Preparation Examples 1 and 2, the sensory evaluation score for the moisturizing feeling and skin condition improvement effect of Preparation Examples 1 and 2 was significantly higher, so the composite extract of Example 3 was applied to actual cosmetics. It is shown that the preference is high by maintaining the functionality even in the

Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments and may be implemented in various different forms, and those skilled in the art to which the present invention belongs can understand the technical spirit or It will be appreciated that it may be embodied in other specific forms without changing its essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (7)

A functional cosmetic composition containing complex extracts of Ecklonia cava, bird's nest, kelp and grapes as active ingredients and having anti-inflammatory effects.
The functional cosmetic composition according to claim 1, wherein the composition further has an effect of improving skin elasticity or skin wrinkles.
The functional cosmetic composition according to claim 1 or 2, wherein the compounding ratio of Ecklonia cava extract, bird's nest extract, kelp extract and grape extract in the composite extract is 3-5: 3-5: 1: 0.5-2.
The functional cosmetic composition according to claim 3, wherein the mixing ratio is 3-5: 3-5: 1: 1.
The cosmetic composition according to claim 1, in the form of one of lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray, and cleanser.
Ecklonia cava extract, bird's nest extract, kelp extract, and grape extract were mixed in a weight ratio of 3-5: 3-5: 1: 0.5-2, or Ecklonia cava, bird's nest, kelp, and grapes were mixed in a weight ratio of 3-5: 3-5: 1 : A method for producing a functional cosmetic composition having anti-inflammatory efficacy, comprising preparing a complex extract by mixing at 0.5 to 2 and extracting by adding a solvent.
The method of claim 6, wherein the extraction method comprises adding 1 to 15 times the solvent to the weight of Ecklonia cava, bird's nest, kelp and grapes, followed by ultrasonic extraction at a temperature of 15 ° C to 25 ° C for 30 minutes to 3 hours. , Method for producing a functional cosmetic composition having an anti-inflammatory effect.