KR20220047879A - 간엽계 줄기세포를 포함한 생체 유래 세포 시료로부터 간엽계 줄기세포를 제조하는 방법 - Google Patents
간엽계 줄기세포를 포함한 생체 유래 세포 시료로부터 간엽계 줄기세포를 제조하는 방법 Download PDFInfo
- Publication number
- KR20220047879A KR20220047879A KR1020227010264A KR20227010264A KR20220047879A KR 20220047879 A KR20220047879 A KR 20220047879A KR 1020227010264 A KR1020227010264 A KR 1020227010264A KR 20227010264 A KR20227010264 A KR 20227010264A KR 20220047879 A KR20220047879 A KR 20220047879A
- Authority
- KR
- South Korea
- Prior art keywords
- mesenchymal stem
- stem cells
- cells
- vitronectin
- partial peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0043—Medium free of human- or animal-derived components
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Rheumatology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JPJP-P-2019-156537 | 2019-08-29 | ||
| JP2019156537 | 2019-08-29 | ||
| JPJP-P-2020-012333 | 2020-01-29 | ||
| JP2020012333 | 2020-01-29 | ||
| PCT/JP2020/032514 WO2021039943A1 (ja) | 2019-08-29 | 2020-08-28 | 間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20220047879A true KR20220047879A (ko) | 2022-04-19 |
Family
ID=74683469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020227010264A Pending KR20220047879A (ko) | 2019-08-29 | 2020-08-28 | 간엽계 줄기세포를 포함한 생체 유래 세포 시료로부터 간엽계 줄기세포를 제조하는 방법 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US12516294B2 (https=) |
| EP (1) | EP4023669A4 (https=) |
| JP (1) | JP7757793B2 (https=) |
| KR (1) | KR20220047879A (https=) |
| CN (1) | CN114286860A (https=) |
| CA (1) | CA3152505A1 (https=) |
| WO (1) | WO2021039943A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230167408A1 (en) * | 2021-11-30 | 2023-06-01 | Trailhead Biosystems Inc. | Methods and compositions for maintaining and expanding hematopoietic stem cells |
| EP4656713A1 (en) * | 2023-01-24 | 2025-12-03 | Ajinomoto Co., Inc. | Cell aggregate dissociating agent |
| DE102023131198B4 (de) * | 2023-11-09 | 2025-11-27 | Anna Höving | Verfahren und Verwendung von serumfreien Medium zur Herstellung von Fleisch |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011111787A1 (ja) | 2010-03-10 | 2011-09-15 | 株式会社ツーセル | 間葉系幹細胞を含む細胞製剤及びその製造方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2399990B1 (en) * | 2003-06-27 | 2015-07-22 | DePuy Synthes Products, Inc. | Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system |
| CA2734446C (en) * | 2008-08-22 | 2017-06-20 | Anthrogenesis Corporation | Methods and compositions for treatment of bone defects with placental cell populations |
| US9133266B2 (en) | 2011-05-06 | 2015-09-15 | Wisconsin Alumni Research Foundation | Vitronectin-derived cell culture substrate and uses thereof |
| WO2015064661A1 (ja) * | 2013-10-31 | 2015-05-07 | 富士フイルム株式会社 | ポリペプチド組成物およびこれを用いた多能性幹細胞の培養方法 |
| US10472605B2 (en) | 2014-02-14 | 2019-11-12 | National University Of Ireland, Galway | Serum-free medium |
| KR102676761B1 (ko) * | 2015-12-03 | 2024-06-18 | 다카라 바이오 가부시키가이샤 | 중간엽 줄기세포의 제조방법 |
| JP7031385B2 (ja) | 2018-03-09 | 2022-03-08 | 株式会社タダノ | クレーン |
| CN108517313B (zh) * | 2018-04-17 | 2021-06-01 | 郭子宽 | 一种用于培养扩增牙髓干细胞的血清/血浆替代物 |
| JP6570152B1 (ja) | 2018-07-20 | 2019-09-04 | 株式会社エムケー技研 | マンホールの吸排気用のチャンバー |
-
2020
- 2020-08-28 CA CA3152505A patent/CA3152505A1/en active Pending
- 2020-08-28 CN CN202080060201.4A patent/CN114286860A/zh active Pending
- 2020-08-28 KR KR1020227010264A patent/KR20220047879A/ko active Pending
- 2020-08-28 EP EP20859384.8A patent/EP4023669A4/en active Pending
- 2020-08-28 JP JP2021543023A patent/JP7757793B2/ja active Active
- 2020-08-28 WO PCT/JP2020/032514 patent/WO2021039943A1/ja not_active Ceased
-
2022
- 2022-02-25 US US17/680,619 patent/US12516294B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011111787A1 (ja) | 2010-03-10 | 2011-09-15 | 株式会社ツーセル | 間葉系幹細胞を含む細胞製剤及びその製造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4023669A1 (en) | 2022-07-06 |
| CA3152505A1 (en) | 2021-03-04 |
| CN114286860A (zh) | 2022-04-05 |
| US20220177848A1 (en) | 2022-06-09 |
| JP7757793B2 (ja) | 2025-10-22 |
| WO2021039943A1 (ja) | 2021-03-04 |
| JPWO2021039943A1 (https=) | 2021-03-04 |
| US12516294B2 (en) | 2026-01-06 |
| EP4023669A4 (en) | 2023-09-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7372910B2 (ja) | ヒト多能性幹細胞由来の胸腺オルガノイドのインビトロ生成 | |
| JP7708651B2 (ja) | 幹細胞の製造に用いられるフィブロネクチンフラグメント | |
| JP2021184754A (ja) | 生体組織から単離できる多能性幹細胞 | |
| EP3580330B1 (en) | Method for generating t cells progenitors | |
| US9597359B2 (en) | Production of dentin, cementum and enamel by cells | |
| Kmiecik et al. | Current view on osteogenic differentiation potential of mesenchymal stromal cells derived from placental tissues | |
| US20150064141A1 (en) | Regenerative sera cells and mesenchymal stem cells | |
| KR102676761B1 (ko) | 중간엽 줄기세포의 제조방법 | |
| CN102803472B (zh) | 去除了外来表型的由多能干细胞分化的子代细胞 | |
| US20120295347A1 (en) | Methods and Compositions for Producing Endothelial Progenitor Cells from Pluripotent Stem Cells | |
| US12516294B2 (en) | Method for producing mesenchymal stem cells from living body-derived cell sample containing mesenchymal stem cells | |
| CN106459904B (zh) | 用于生成内皮集落形成细胞状细胞的方法 | |
| KR20140143363A (ko) | 기질 줄기 세포 | |
| CN102933704A (zh) | 软骨祖细胞、用于细胞衍生的方案和其用途 | |
| US20140147419A1 (en) | Compositions and methods for formation of bone tissue | |
| JP2017531448A (ja) | T細胞前駆細胞を生成するための方法 | |
| CA2908225A1 (en) | Method for culturing hepatoblast-like cells and culture product thereof | |
| Xiong et al. | Recapitulation of endochondral ossification by hPSC-derived SOX9+ sclerotomal progenitors | |
| AU2023353929A1 (en) | T cell production method | |
| Liu et al. | A synthetic substrate to support early mesodermal differentiation of human embryonic stem cells | |
| US12018276B2 (en) | Lymphocyte production method | |
| Ghoneim et al. | Isolation of bone Marrow and adipose-derived mesenchymal stromal cells | |
| US20240344031A1 (en) | Mesenchymal stem cell line | |
| JPWO2019189947A1 (ja) | ヒト臍帯由来間葉系幹細胞から骨芽細胞の製造を目的としたアクチン重合阻害剤による分化誘導技術 | |
| Sachenberg et al. | Spreading and actin cytoskeleton organization of cartilage and bone marrow stromal cells cocultured on various extracellular matrix proteins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
|
| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
|
| A201 | Request for examination | ||
| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |
|
| R18-X000 | Changes to party contact information recorded |
St.27 status event code: A-3-3-R10-R18-oth-X000 |
|
| D21 | Rejection of application intended |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |
|
| E13 | Pre-grant limitation requested |
Free format text: ST27 STATUS EVENT CODE: A-2-3-E10-E13-LIM-X000 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |