JP7757793B2 - 間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法 - Google Patents
間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法Info
- Publication number
- JP7757793B2 JP7757793B2 JP2021543023A JP2021543023A JP7757793B2 JP 7757793 B2 JP7757793 B2 JP 7757793B2 JP 2021543023 A JP2021543023 A JP 2021543023A JP 2021543023 A JP2021543023 A JP 2021543023A JP 7757793 B2 JP7757793 B2 JP 7757793B2
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- Prior art keywords
- mesenchymal stem
- stem cells
- vitronectin
- cells
- partial peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
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- C12N2500/00—Specific components of cell culture medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2513/00—3D culture
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0043—Medium free of human- or animal-derived components
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Rheumatology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019156537 | 2019-08-29 | ||
| JP2019156537 | 2019-08-29 | ||
| JP2020012333 | 2020-01-29 | ||
| JP2020012333 | 2020-01-29 | ||
| PCT/JP2020/032514 WO2021039943A1 (ja) | 2019-08-29 | 2020-08-28 | 間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPWO2021039943A1 JPWO2021039943A1 (https=) | 2021-03-04 |
| JP7757793B2 true JP7757793B2 (ja) | 2025-10-22 |
Family
ID=74683469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021543023A Active JP7757793B2 (ja) | 2019-08-29 | 2020-08-28 | 間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US12516294B2 (https=) |
| EP (1) | EP4023669A4 (https=) |
| JP (1) | JP7757793B2 (https=) |
| KR (1) | KR20220047879A (https=) |
| CN (1) | CN114286860A (https=) |
| CA (1) | CA3152505A1 (https=) |
| WO (1) | WO2021039943A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230167408A1 (en) * | 2021-11-30 | 2023-06-01 | Trailhead Biosystems Inc. | Methods and compositions for maintaining and expanding hematopoietic stem cells |
| EP4656713A1 (en) * | 2023-01-24 | 2025-12-03 | Ajinomoto Co., Inc. | Cell aggregate dissociating agent |
| DE102023131198B4 (de) * | 2023-11-09 | 2025-11-27 | Anna Höving | Verfahren und Verwendung von serumfreien Medium zur Herstellung von Fleisch |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015064661A1 (ja) | 2013-10-31 | 2015-05-07 | 富士フイルム株式会社 | ポリペプチド組成物およびこれを用いた多能性幹細胞の培養方法 |
| JP2017506511A (ja) | 2014-02-14 | 2017-03-09 | ナショナル ユニバーシティー オブ アイルランド, ゴールウェイ | 無血清培地 |
| WO2017094879A1 (ja) | 2015-12-03 | 2017-06-08 | タカラバイオ株式会社 | 間葉系幹細胞の製造方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2399990B1 (en) * | 2003-06-27 | 2015-07-22 | DePuy Synthes Products, Inc. | Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system |
| CA2734446C (en) * | 2008-08-22 | 2017-06-20 | Anthrogenesis Corporation | Methods and compositions for treatment of bone defects with placental cell populations |
| CA2792802C (en) | 2010-03-10 | 2017-10-31 | Two Cells Co., Ltd. | Cell preparation containing mesenchymal stem cells, and method for producing same |
| US9133266B2 (en) | 2011-05-06 | 2015-09-15 | Wisconsin Alumni Research Foundation | Vitronectin-derived cell culture substrate and uses thereof |
| JP7031385B2 (ja) | 2018-03-09 | 2022-03-08 | 株式会社タダノ | クレーン |
| CN108517313B (zh) * | 2018-04-17 | 2021-06-01 | 郭子宽 | 一种用于培养扩增牙髓干细胞的血清/血浆替代物 |
| JP6570152B1 (ja) | 2018-07-20 | 2019-09-04 | 株式会社エムケー技研 | マンホールの吸排気用のチャンバー |
-
2020
- 2020-08-28 CA CA3152505A patent/CA3152505A1/en active Pending
- 2020-08-28 CN CN202080060201.4A patent/CN114286860A/zh active Pending
- 2020-08-28 KR KR1020227010264A patent/KR20220047879A/ko active Pending
- 2020-08-28 EP EP20859384.8A patent/EP4023669A4/en active Pending
- 2020-08-28 JP JP2021543023A patent/JP7757793B2/ja active Active
- 2020-08-28 WO PCT/JP2020/032514 patent/WO2021039943A1/ja not_active Ceased
-
2022
- 2022-02-25 US US17/680,619 patent/US12516294B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015064661A1 (ja) | 2013-10-31 | 2015-05-07 | 富士フイルム株式会社 | ポリペプチド組成物およびこれを用いた多能性幹細胞の培養方法 |
| JP2017506511A (ja) | 2014-02-14 | 2017-03-09 | ナショナル ユニバーシティー オブ アイルランド, ゴールウェイ | 無血清培地 |
| WO2017094879A1 (ja) | 2015-12-03 | 2017-06-08 | タカラバイオ株式会社 | 間葉系幹細胞の製造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4023669A1 (en) | 2022-07-06 |
| CA3152505A1 (en) | 2021-03-04 |
| CN114286860A (zh) | 2022-04-05 |
| US20220177848A1 (en) | 2022-06-09 |
| WO2021039943A1 (ja) | 2021-03-04 |
| KR20220047879A (ko) | 2022-04-19 |
| JPWO2021039943A1 (https=) | 2021-03-04 |
| US12516294B2 (en) | 2026-01-06 |
| EP4023669A4 (en) | 2023-09-13 |
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