CA3152505A1 - Method for producing mesenchymal stem cells from living body-derived cell sample containing mesenchymal stem cells - Google Patents

Method for producing mesenchymal stem cells from living body-derived cell sample containing mesenchymal stem cells Download PDF

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Publication number
CA3152505A1
CA3152505A1 CA3152505A CA3152505A CA3152505A1 CA 3152505 A1 CA3152505 A1 CA 3152505A1 CA 3152505 A CA3152505 A CA 3152505A CA 3152505 A CA3152505 A CA 3152505A CA 3152505 A1 CA3152505 A1 CA 3152505A1
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Canada
Prior art keywords
mesenchymal stem
stem cells
vitronectin
cells
partial peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3152505A
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English (en)
French (fr)
Inventor
Ikue HARATA
Eri Harada
Kazuma Takahashi
Fumie Honkawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
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Ajinomoto Co Inc
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Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of CA3152505A1 publication Critical patent/CA3152505A1/en
Pending legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2513/00—3D culture
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    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3152505A 2019-08-29 2020-08-28 Method for producing mesenchymal stem cells from living body-derived cell sample containing mesenchymal stem cells Pending CA3152505A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2019156537 2019-08-29
JP2019-156537 2019-08-29
JP2020012333 2020-01-29
JP2020-012333 2020-01-29
PCT/JP2020/032514 WO2021039943A1 (ja) 2019-08-29 2020-08-28 間葉系幹細胞を含む生体由来細胞試料から間葉系幹細胞を製造する方法

Publications (1)

Publication Number Publication Date
CA3152505A1 true CA3152505A1 (en) 2021-03-04

Family

ID=74683469

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3152505A Pending CA3152505A1 (en) 2019-08-29 2020-08-28 Method for producing mesenchymal stem cells from living body-derived cell sample containing mesenchymal stem cells

Country Status (7)

Country Link
US (1) US12516294B2 (https=)
EP (1) EP4023669A4 (https=)
JP (1) JP7757793B2 (https=)
KR (1) KR20220047879A (https=)
CN (1) CN114286860A (https=)
CA (1) CA3152505A1 (https=)
WO (1) WO2021039943A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230167408A1 (en) * 2021-11-30 2023-06-01 Trailhead Biosystems Inc. Methods and compositions for maintaining and expanding hematopoietic stem cells

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4656713A1 (en) * 2023-01-24 2025-12-03 Ajinomoto Co., Inc. Cell aggregate dissociating agent
DE102023131198B4 (de) * 2023-11-09 2025-11-27 Anna Höving Verfahren und Verwendung von serumfreien Medium zur Herstellung von Fleisch

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2399990B1 (en) * 2003-06-27 2015-07-22 DePuy Synthes Products, Inc. Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system
CA2734446C (en) * 2008-08-22 2017-06-20 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
CA2792802C (en) 2010-03-10 2017-10-31 Two Cells Co., Ltd. Cell preparation containing mesenchymal stem cells, and method for producing same
US9133266B2 (en) 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof
WO2015064661A1 (ja) * 2013-10-31 2015-05-07 富士フイルム株式会社 ポリペプチド組成物およびこれを用いた多能性幹細胞の培養方法
US10472605B2 (en) 2014-02-14 2019-11-12 National University Of Ireland, Galway Serum-free medium
KR102676761B1 (ko) * 2015-12-03 2024-06-18 다카라 바이오 가부시키가이샤 중간엽 줄기세포의 제조방법
JP7031385B2 (ja) 2018-03-09 2022-03-08 株式会社タダノ クレーン
CN108517313B (zh) * 2018-04-17 2021-06-01 郭子宽 一种用于培养扩增牙髓干细胞的血清/血浆替代物
JP6570152B1 (ja) 2018-07-20 2019-09-04 株式会社エムケー技研 マンホールの吸排気用のチャンバー

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230167408A1 (en) * 2021-11-30 2023-06-01 Trailhead Biosystems Inc. Methods and compositions for maintaining and expanding hematopoietic stem cells

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Publication number Publication date
EP4023669A1 (en) 2022-07-06
CN114286860A (zh) 2022-04-05
US20220177848A1 (en) 2022-06-09
JP7757793B2 (ja) 2025-10-22
WO2021039943A1 (ja) 2021-03-04
KR20220047879A (ko) 2022-04-19
JPWO2021039943A1 (https=) 2021-03-04
US12516294B2 (en) 2026-01-06
EP4023669A4 (en) 2023-09-13

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