KR20210048137A - Cosmetic Composition for Improving Skin Condition Comprising Fermentation Product of Red Ginseng Oil - Google Patents

Abstract

The present invention provides a cosmetic composition for improving skin conditions comprising a fermented product of red ginseng oil. The cosmetic composition of the present invention contains the fermented product of red ginseng oil as a kind of biological surfactants, so the cosmetic composition not only has excellent stability, but also has excellent skin condition improvement effects such as moisturizing properties, elasticity improvement and dead cell skin removal.

Description

Cosmetic Composition for Improving Skin Condition Comprising Fermentation Product of Red Ginseng Oil}

The present invention relates to a cosmetic composition for improving skin condition comprising a fermented product of red ginseng oil.

As the largest organ of our body, the skin occupies about 16% of the total volume of our body, and while in direct contact with the external environment, it functions as a barrier to protect the human body from many deadly harmful factors that try to invade into the body and external stimuli. The barrier function is a protective function that protects against various external stimuli such as chemicals, air pollutants, dry environment, ultraviolet rays, etc., and prevents excessive dissipation of moisture in the body through the skin.This protective function is the stratum corneum composed of keratinocytes. It can only maintain its function if it is normally formed.

The stratum corneum in the outermost layer of the epidermis is formed from keratinocytes, and is composed of keratinocytes that have been differentiated and the lipid membrane between keratinocytes surrounding them. Keratinocytes are characteristic cells in which basal cells that continuously proliferate in the lowermost layer of the epidermis go through a step-by-step change in shape and function and rise to the surface of the skin.After a certain period of time, old keratinocytes are eliminated from the skin and new keratinocytes function. This is called "differentiation of epidermal cells" or "keratinization". In addition, during the keratinization process, the keratinocytes form a stratum corneum while generating natural moisturizing factors and intercellular lipids, thereby making the stratum corneum firm and flexible, thereby retaining the function as a skin barrier. The intercellular lipid is composed of ceramide, cholesterol, and free fatty acids, and they each account for about 40-65%, 10%, and 25% of the total lipid in the stratum corneum. In addition, it has a composition in which a small amount of phytosphingosine and sphingosine are present together.

As we age, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the dermal matrix, which plays an important role in maintaining skin elasticity, decreases, and the amount of insoluble collagen increases, making it difficult for the dermis to retain moisture in the skin, and the skin becomes rough. do. In addition, as skin aging progresses, cell division is not well performed, so the keratinization cycle is delayed and becomes uneven, and the skin barrier function of the stratum corneum is deteriorated. In addition, the function of the sebaceous glands and sweat glands is also reduced, so that the amount of sebum and sweat gradually decreases, and the sebaceous membrane is not properly formed, so the protective ability for moisturizing the skin is lost, eventually, the skin becomes dry and loses transparency. The elasticity decreases and wrinkles are formed. In other words, loss of moisturizing power of the skin is a major symptom of aging, along with a decrease in skin elasticity and an increase in skin keratin, and is closely related.

In addition, aging and the stratum corneum can easily lose their functions due to lifestyle factors such as excessive washing or bathing, or environmental factors such as dry air pollutants. There is a trend of increasing dry skin symptoms and symptoms resulting from this.

In order to suppress such skin aging or to alleviate the speed of progress, cosmetic compositions for moisturizing skin, improving elasticity, or improving dead skin cells such as lotions, creams, or oil formulations have been developed. Surfactants are commonly used in such lotions, creams, or oil formulations, but due to the ability to lower the surface tension through the activation of the interface between the phases, the surface is wetted, penetrated, air bubbles, defoamed, emulsified, solubilized, and dispersed. It is a component widely used not only in cosmetics, but also in foods, pharmaceuticals, detergents, etc., as it has functions such as coagulation and washing.

The surfactants are largely divided into synthetic surfactants, natural surfactants, and biological surfactants. Until only a few years ago, synthetic surfactants were widely used for reasons such as cost, but recently, natural surfactants were known that the main ingredients of synthetic surfactants give strong irritation to human skin and can cause skin diseases such as atopy and allergies. Or, the use of biological surfactants is increasing. However, these natural surfactants or biological surfactants not only have disadvantages in terms of cost compared to synthetic surfactants, but also have a problem in that they are difficult to use as a single emulsifier due to their low emulsifying power. Therefore, studies on natural materials and strains have been continuously conducted in order to produce natural surfactants or biological surfactants that can be used as a single emulsifier with improved emulsification power.

Ginseng (Panax ginseng CA Meyer) is a perennial herb belonging to the genus Panax of Araliaceae. It is one of the herbal medicines used in oriental medicine. It has a sweet and bitter taste, has a warm energy, rejuvenates and protects the spleen. It has herbal effects such as strengthening the lungs.

The main physiologically active substances of ginseng include saponins, also called ginsenosides, essential oils, polyacetylene, phenolic components, glycosides and acidic peptides. In addition, various components such as vitamins, sugars, and minerals are contained. The physiological activity of ginseng has been reported to have a central nervous system effect, an immune function strengthening effect, and an anticancer effect. In particular, about 40 species of ginsenosides have been discovered so far, and it has been confirmed to have an excellent effect in regulating body functions, that is, normalizing physiological functions by exerting a wide range of effects on the central nervous system, endocrine system, immune system, and metabolic system. These ginsenosides have similar or opposite actions to each other, but it is known that specific ingredients exert various effects either alone or through interactions of several types.

The present inventors found that in the case of a fermented product obtained by fermenting red ginseng oil using a specific strain, the emulsifying power is improved, so that the stability of the composition containing the same is improved, and the composition has excellent skin moisturization, skin elasticity improvement, and skin keratin improvement effects. Confirmed and completed the present invention.

The present invention provides a cosmetic composition that includes a bio-surfactant developed as an alternative to the conventional synthetic surfactant, not only has excellent stability of the cosmetic composition, but also has excellent effects on improving skin conditions such as moisturizing, improving elasticity, and improving dead skin cells. It is aimed at.

One embodiment of the present invention is a cosmetic composition for skin moisturizing comprising a fermented red ginseng oil fermented from red ginseng oil with a Candida bombicola strain, such as Candida bombicola UA-06, deposited under the accession number KCTC18592P. The composition is provided.

Another embodiment of the present invention provides a cosmetic composition for improving skin elasticity comprising a fermented product of red ginseng oil fermented with a Candida bombicola strain, such as a Candida bombicola UA-06 strain deposited with the deposit number KCTC18592P.

Another embodiment of the present invention provides a cosmetic composition for improving skin keratin comprising a fermented product of red ginseng oil fermented with a Candida bombicola strain, such as a Candida bombicola UA-06 strain deposited under the accession number KCTC18592P.

The present invention includes a fermented product of red ginseng oil as a kind of biological surfactant, thereby improving the emulsifying power of the biological surfactant to improve the stability of the cosmetic composition, as well as improving the skin condition such as moisturizing, elasticity and dead skin cells. It can provide an excellent cosmetic composition.

1 shows the skin moisturizing effect of a composition containing a fermented red ginseng oil as an embodiment of the present invention.
2 shows the effect of improving skin elasticity of a composition comprising fermented red ginseng oil as an embodiment of the present invention.
3 shows the effect of improving skin keratin of a composition containing fermented red ginseng oil as an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail to aid understanding of the present invention.

The terms or words used in the specification and claims should not be construed as being limited to their usual or dictionary meanings, and the inventor may appropriately define the concept of terms in order to describe his own invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

1. Manufacture of fermented red ginseng oil

The fermented product of red ginseng oil of the present invention can be prepared by fermenting red ginseng oil with a Candida bombicola strain, such as a Candida bombicola UA-06 strain deposited under the accession number KCTC18592P.

The red ginseng oil is an oil extracted by any method from red ginseng or red ginseng leaf, and the red ginseng is obtained by steaming ginseng. The ginseng is Korean ginseng ( Panax ginseng ), Hoegi ginseng (P. quiquefolius ), Jeonchil ginseng (P. notoginseng ), jukjeolsam (P. japonicus ), trifolium ginseng (P. trifolium ), Himalayan ginseng (P. pseudoginseng ), Vietnamese ginseng. ( P. vietnamensis ), or American ginseng (P. quinquefolium ), and the like. Ginseng is generally classified into white ginseng and red ginseng according to the processing method, and white ginseng refers to unprocessed ginseng mined in the field, that is, fresh ginseng as it is dried, and red ginseng is dried by steaming fresh ginseng. Several chemical changes are involved, including amino acid changes. Red ginseng produces ginsenosides that do not exist in ginseng by the heat applied during the manufacturing process, and the unique active ingredient of red ginseng has cancer prevention action, cancer cell growth inhibition action, blood pressure lowering action, brain nerve cell protection, and learning ability improvement action. Show. In the present invention, red ginseng is a ginseng leaf, young sprout, stem, stem bark, root, root bark, seed, fruit, immature fruit, ripe fruit, flesh, pericarp, flower, stamen group (androecium), pistil group (gynoecium), It can be obtained by steaming any one selected from the group consisting of calyxes, stamens, petals, calyx pieces, carpels, and combinations thereof, and a specific process may be carried out in a conventional manner.

The red ginseng leaf refers to a by-product generated after extracting and using red ginseng obtained by steaming and/or drying ginseng with water or an organic solvent, and may include a red ginseng leaf powder formulation. The red ginseng leaf powder may be obtained in a powder form by washing the red ginseng leaf with water and then drying it with hot air.

The method of obtaining red ginseng oil from the red ginseng may be carried out by a conventional method, and is not particularly limited. As an example, the red ginseng oil may be extracted by treating red ginseng or red ginseng leaf with an organic solvent, or by separating and extracting a fat-soluble component contained in red ginseng by supercritical fluid extraction.

When extracted with an organic solvent, the organic solvent is an alcohol, preferably a C1-C4 lower alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, It may be any one selected from the group consisting of chloroform, ethyl acetate, benzene, and a mixed solvent thereof. In addition, the extraction method may be a solvent extraction method or a reflux extraction method, but is not particularly limited thereto.

In the case of extraction with a supercritical fluid, water or carbon dioxide may be used as the supercritical fluid, but is not particularly limited thereto. As conditions for supercritical fluid extraction, temperature, time, pressure, etc. can be controlled during supercritical extraction, and the conditions will be described in detail below.

When extracted with the supercritical fluid, the temperature may be 40°C to 100°C, preferably 50°C to 90°C, but is not limited thereto. If the temperature during extraction is out of the appropriate range, the yield of the extract decreases, and the production cost increases, thereby increasing the cost of the product.

When extracted with a supercritical fluid, the extraction time may be 0.5 to 10 hours, preferably 1 to 8 hours, but is not limited thereto.

In addition, conditions during supercritical extraction may maintain the pressure at 10Mpa to 50Mpa, preferably 15Mpa to 45Mpa, but are not limited thereto.

The red ginseng oil may increase the content of fat-soluble components by performing a conventional moisture removal process in order to remove moisture that may be included during the extraction process. For example, the red ginseng oil according to the present invention can be used to remove moisture contained in the oil using a vacuum rotary concentrator.

The red ginseng oil may be obtained by performing a conventional fractionation process to obtain a fraction. For example, a fraction obtained by passing the red ginseng oil according to the present invention through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity) The active fraction obtained through various purification methods additionally carried out such as, etc. are also included in the red ginseng oil of the present invention. By separating red ginseng oil in which several ingredients are mixed according to the properties of the active ingredient through concentration gradient column chromatography, etc., a specific active fraction having a higher preventive or therapeutic effect on prostatitis can be prepared. Column chromatography may be performed several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, the elution solvent, the elution rate, and the elution time may be applied to a solvent, speed, or time generally used in the art.

In the present invention, the fermented product of red ginseng oil may be prepared using a Candida bombicola strain, for example, a Candida bombicola UA-06 strain deposited under the accession number KCTC18592P. Specifically, the fermented product of red ginseng oil is 1) a pre-cultivation step of culturing a Candida bombicola strain, such as a Candida bombicola UA-06 strain deposited with an accession number KCTC18592P; And 2) culturing the pre-cultured strain and red ginseng oil.

The 1) pre-cultivation step includes 1-1) a first pre-cultivation step of culturing the Candida bombicola UA-06 strain deposited with the accession number KCTC18592P in a medium; And 1-2) a second pre-culture step of culturing the first pre-cultured strain in a medium.

In the 1-1) first pre-culture step, the medium may contain at least one selected from the group consisting of yeast extract, malt extract, peptone, glucose, and urea. In addition, in the 1-2) second pre-culture step, the medium may contain at least one selected from the group consisting of yeast extract, malt extract, peptone, glycerin, and urea.

The first pre-culture step and the second pre-culture step may each independently be performed at 15 to 30° C. for 10 to 30 hours. The pre-culture temperature may be 20 to 25°C, and the pre-culture time may be 22 to 28 hours, or 24 to 26 hours.

In addition, the 2) culturing step, 2-1) a first culturing step of culturing the second pre-cultured strain and red ginseng oil; And 2-2) a second culture step of culturing by adding red ginseng oil to the fermented product of the first cultured red ginseng oil. In the culture step of 2-1) or 2-2), the medium is glycerin, sodium glutamate, sodium citrate, yeast extract, KH ₂ PO ₄ , MgCl ₂ and MnCl _2, It may include one or more selected from the group consisting of.

The first culturing step and the second culturing step may be performed independently at 20 to 40° C. for 72 to 150 hours. The pre-culture temperature may be 23 to 35°C or 24 to 30°C, and the pre-culture time may be 80 to 130 hours, or 90 to 125 hours.

The red ginseng oil fermented product thus formed may include at least one selected from the group consisting of glycero glycolipide, glycolipide, and glycerolipide.

2. Preparation of cosmetic composition

The cosmetic composition of the present invention has a skin condition improvement effect by including a fermented product of red ginseng oil fermented with a Candida bombicola strain, such as a Candida bombicola UA-06 strain deposited under the accession number KCTC18592P.

The description of the fermented red ginseng oil is the same as described in '1. Preparation of fermented red ginseng oil ', so a detailed description will be omitted.

In the present invention, the improvement of the skin condition may mean moisturizing, improving elasticity, or improving keratin.

The cosmetic composition of the present invention can maintain stability by including the fermented red ginseng oil as a single emulsifier as a biological surfactant, and thus sodium lauryl sulfate, sodium laureth sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, glyceryl Stearate, PEG-100 stearate, PEG-40 stearate, polyglyceryl-3methylglucose distearate, sucrosepolytearate, sorbitan stearate, cetearylolibate, sorbitanolibate, lecithin and hydro It is characterized in that it does not contain at least one surfactant selected from the group consisting of genated lecithin.

The cosmetic composition of the present invention contains the fermented red ginseng oil in an amount of 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, or 0.5 to 1.5% by weight, based on the total weight of the cosmetic composition. Can include.

The cosmetic composition of the present invention may be prepared in a liquid or solid form using bases, auxiliary agents, and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, for example, but not limited to, a form such as a lotion, cream, lotion, and bath agent.

Bases, auxiliary agents and additives commonly used in the cosmetic field are not particularly limited, and examples include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

When the composition of the present invention is prepared as a cosmetic composition, the composition of the present invention may include not only red ginseng oil fermented products, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, Conventional adjuvants such as pigments and fragrances, and carriers may be included.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation , Emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's skin condition or taste.

When the cosmetic composition of the present invention is a soap or cleansing formulation, it may be wiped off, removed, or washed off with water after being applied to the skin. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, and the cleansing formulation is a cleansing foam, cleansing water, cleansing towel, cleansing pack, cleansing cream, cleansing lotion, cleansing water and cleansing gel. It is not limited.

Hereinafter, the present invention will be described in detail by way of manufacturing examples and examples.

However, the following Preparation Examples and Examples are for illustrative purposes only, and the contents of the present invention are not limited by the following Preparation Examples and Examples.

Preparation Example 1. Preparation of fermented red ginseng oil

1. First pre-culture of strain

Candida bombicola UA-06 (KCTC18592P) strain was inoculated into 1 L of medium, and the first pre-culture of the strain was performed for 24 hours under aerobic conditions of 25° C., 300 rpm, and 1 NL/min (1 vvm). In order to optimize the growth and metabolism process of microorganisms, the culture medium used was yeast extract 3g/L, malt extract 3g/L, peptone 5g/L, glucose 10g/L, urea 1g/L, and distilled water 978g/L composition.

2. Second pre-culture of strain

Except for substituting 10 g/L of glucose with 20 g/L of glycerin in the first pre-culture medium composition, 10 L of the culture medium having the same composition as the first pre-culture medium contains 25° C., 300 rpm, 10 NL/min (1 vvm) A second pre-culture was carried out for 24 hours under normal conditions.

3. The first culture of red ginseng oil

The second pre-cultured culture medium was glycerin 30g/L, sodium glutamate 5g/L, sodium citrate 1g/L, yeast extract 2g/L, KH ₂ PO ₄ 7g/L, MgCl ₂ 0.5g/L, MnCl ₂ 0.2 g/L, distilled water 904.3g/L, red ginseng oil (KGC Ginseng Corporation) 50g/L of 50g/L culture medium incubated for 96 hours at 30℃, 300rpm, 100NL/min (1vvm) aerobic conditions. The first culture was performed.

4. Second culture of red ginseng oil

The first cultured culture medium was glycerin 10g/L, sodium glutamate 20g/L, sodium citrate 5g/L, yeast extract 2g/L, KH ₂ PO ₄ 7g/L, MgCl ₂ 0.5g/L, MnCl ₂ 0.2g/ L, distilled water 455.3g/L, red ginseng oil (KGC Ginseng Corporation) 500g/L of 500g/L culture medium incubated under aerobic conditions of 25℃, 300rpm, 100NL/min (1vvm), and the second red ginseng oil for about 120 hours. Culture was carried out.

5. Tablets

In order to remove moisture and microorganisms, the second cultured culture solution was allowed to stand and separated into an upper layer (fermented product) and a lower layer (medium and impurities), and then all the lower layers were discarded, and MgSO ₄ 20 g/to remove moisture. L was added and stirred. After 2 hours of stirring, microbial cells, MgSO ₄ , and impurities remaining in the upper layer were removed using a continuous centrifuge equipment. In addition, the fermented product from which moisture has been removed is heated to 60 to 70°C and used in an affinity chromatography column filled with magnesium silicate to adsorb and filter fatty acids and impurities, and finally fermented red ginseng oil Obtained.

Example 1. Preparation of essence containing fermented red ginseng oil

① The essence for external use was prepared according to the composition of Table 1 below.

② Mix 2 with raw materials 1 in Table 1 below, stir and loosen well.

③ Put raw materials 3~6 in Table 1 below into ②, mix well, and heat to 75℃.

④ Put the raw materials 7~9 in Table 1 below into a separate container and heat it to 75℃.

⑤ Put ④ into the reaction vessel of ③ and emulsify.

⑥ Add raw material 10 from Table 1 below and neutralize it.

⑦ Ingredients 11 to 12 of Table 1 are added, mixed, and then cooled to 30°C.

division Raw material name Content (% by weight) One Purified water Balance 2 Carbomer 0.20 3 Disodium EDTA 0.02 4 Butylene Glycol 5.00 5 glycerin 7.00 6 Panthenol 1.00 7 Red ginseng oil fermented product of Preparation Example 1 3.00 8 Dimethicone 1.00 9 Caprylyl/Capric Triglyceride 2.00 10 Tromethamine 0.02 11 antiseptic Appropriate amount 12 incense Appropriate amount Sum 100.00

Comparative Example 1. Preparation of Essence Containing Rice/Coconut Oil Fermented Product

In Preparation Example 1, rice/coconut oil fermented products were prepared in the same manner, except that rice/coconut oil was used instead of red ginseng oil.

Ⓐ Essence for external use was prepared according to the composition of Table 2 below.

Ⓑ Mix the raw materials 1 and 2 in Table 2 below, stir and loosen well.

Ⓒ Put raw materials 3~6 in Table 2 below into ⓑ, mix well, and heat to 75℃.

Ⓓ Put the raw materials 7~9 of Table 2 in a separate container and heat it to 75℃.

Put ⓓ into the reaction vessel of ⓔ ⓒ to emulsify.

Ⓕ Put the raw material 10 in Table 2 below and neutralize it.

Ⓖ Add the raw materials 11 to 12 of Table 2 below, mix, and cool to 30℃.

division Raw material name Content (% by weight) One Purified water Balance 2 Carbomer 0.20 3 Disodium EDTA 0.02 4 Butylene Glycol 5.00 5 glycerin 7.00 6 Panthenol 1.00 7 Rice/Coconut Oil Fermented Product 3.00 8 Dimethicone 1.00 9 Caprylyl/Capric Triglyceride 2.00 10 Tromethamine 0.02 11 antiseptic Appropriate amount 12 incense Appropriate amount Sum 100.00

Experimental Example 1. Evaluation of skin moisturizing power

The essence of Example 1 and Comparative Example 1 was applied to the forearm twice a day in the morning and evening for 2 weeks. Thereafter, the moisturizing power was measured once a week for 2 weeks, and the results are shown in Table 3 and FIG. 1. Moisturizing power was measured by measuring electrical conductivity using Corneometer CM825 (Courage+Khazaka, Germany), and the result is expressed in arbitrary units. The improvement rate represents the rate of change compared to the first measurement value in %.

Moisturizing power (Arbitrary unit) Improvement rate (%)
Example 1 Comparative Example 1 Example 1 Comparative Example 1 1 time 43.83 46.34 - - Episode 2 50.05 46.27 12.43 -0.16 3rd time 53.4 47.64 17.93 2.73

In FIG. 1, it can be seen that the essence of Example 1 has a very good moisturizing effect compared to the essence of Comparative Example 1.

Experimental Example 2. Evaluation of skin elasticity

After applying the essence of Example 1 and Comparative Example 1 to the forearm twice a day in the morning and evening for 2 weeks, the skin elasticity was measured once a week for 2 weeks, and the results are shown in Table 4 and FIG. 2. Measurement of skin elasticity was performed by measuring the rate of restoration after applying negative pressure to the skin using Cutomter MPA580 (Courage+Khazaka, Germany), and the result is an R7 value, expressed in arbitrary units. The improvement rate represents the rate of change compared to the first measurement value in %.

Skin elasticity (R7 value, Arbitrary unit) Improvement rate (%)
Example 1 Comparative Example 1 Example 1 Comparative Example 1 1 time 0.601 0.632 - - Episode 2 0.634 0.621 5.244 -1.853 3rd time 0.681 0.658 11.781 3.955

In FIG. 2, it can be seen that the essence of Example 1 has a very excellent effect of improving skin elasticity compared to the essence of Comparative Example 1.

Experimental Example 3. Skin keratin evaluation

After applying the essence of Example 1 and Comparative Example 1 to the forearm area twice a day in the morning and in the evening for 2 weeks, skin keratin was measured once a week for 2 weeks, and the results are shown in Table 5 and FIG. 3. Skin keratin was measured by collecting keratin from the test site using Corneofix and analyzing the image after taking a picture using a Skin Visiometer SV600 (Courage+Khazaka, Germany). It is represented by (Arbitrary unit). The improvement rate represents the rate of change compared to the first measurement value in %.

Desquamation index, Arbitrary unit Improvement rate (%)
Example 1 Comparative Example 1 Example 1 Comparative Example 1 1 time 63.52 61.43 - - Episode 2 62.47 61.6 1.66 -0.28 3rd time 61.78 61.07 2.74 0.59

In FIG. 3, it can be seen that the essence of Example 1 has a very excellent effect of improving skin keratin compared to the essence of Comparative Example 1.

Preparation Example 2. Preparation of flexible cosmetic water

The flexible cosmetic water containing the fermented red ginseng oil of Preparation Example 1 was prepared as shown in Table 6 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDTA 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Carbomer 0.10 Tromethamine 0.10 Glyceres-26 1.00 Arbutin 2.00 glycerin 3.00 ethanol 3.00 Red ginseng oil fermented product of Preparation Example 1 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

Preparation Example 3. Preparation of nourishing cream

The nutritional cream containing the fermented red ginseng oil of Preparation Example 1 was prepared as shown in Table 7 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium aluminum silicate 0.3 Shea Butter 2.0 Stearic Acid 2.0 Carnauba wax 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Red ginseng oil fermented product of Preparation Example 1 2.5 vegetable oil 1.8 Dimethicone 1.5 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Carbomer Appropriate amount Tromethamine Appropriate amount Flavoring agent Appropriate amount Purified water Balance

Preparation Example 4. Preparation of lotion

A lotion containing the fermented red ginseng oil of Preparation Example 1 was prepared as shown in Table 8 below.

Raw material Content (parts by weight) Caprylyl/Capric Triglyceride 3.0 Dimethicone 2.0 Dipotassium glycyrrhizate a very small amount Squalene 4.8 Macadamia oil 2.0 Jojoba Oil 2.0 Glyceres-26 5.0 glycerin 5.0 1,3-butylene glycol 4.0 Xanthan gum 0.1 d-pandenol 0.5 Red ginseng oil fermented product of Preparation Example 1 2.0 Allantoin 0.1 Carbomer 0.20 Triethanolamine 0.20 Purified water Balance

Claims (6)

A cosmetic composition for improving skin condition, comprising a fermented red ginseng oil fermented from red ginseng oil with Candida bombicola strain. The method according to claim 1,
The Candida bombicola strain is a cosmetic composition that is a Candida bombicola UA-06 strain deposited under the accession number KCTC18592P. The method according to claim 1,
The cosmetic composition is sodium lauryl sulfate, sodium laureth sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, glyceryl stearate, PEG-100 stearate, PEG-40 stearate, polyglyceryl-3methylglucose distearate. A cosmetic composition that does not contain one or more surfactants selected from the group consisting of rate, sucrose polytearate, sorbitan stearate, cetearylolibate, sorbitanolibate, lecithin, and hydrogenated lecithin. The method according to claim 2,
The fermented red ginseng oil is 1) a pre-cultivation step of culturing the Candida bombicola UA-06 strain deposited with the accession number KCTC18592P; And
2) a cultivation step of culturing the pre-cultured strain and red ginseng oil; a cosmetic composition prepared by a manufacturing method comprising. The method according to claim 1,
The red ginseng oil fermented product is a cosmetic composition comprising at least one selected from the group consisting of glycero glycolipide, glycolipide, and glycerolipide. The method according to claim 1,
The skin condition improvement is a cosmetic composition selected from the group consisting of skin moisturizing improvement, skin elasticity improvement, and skin keratin improvement.

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