KR20210046210A - Eco-friendly sterilizer - Google Patents

Abstract

The present invention relates to a natural disinfectant containing distilled water obtained by distilling ginkgo leaves, bamboo leaves, Artemisia japonica leaves, Boehmeria nivea leaves, green tea leaves and pine needles, and more particularly, to a disinfectant for air, a humidifier or drinking water containing 100% colorless distilled water obtained by distilling a mixture of ginkgo leaves, bamboo leaves, Artemisia japonica leaves, Boehmeria nivea leaves, green tea leaves and pine needles with the ratio of 1.5-2.5 : 1.0-2.0 : 1.5-2.5 : 1.0-2.0 : 1.5-2.5 : 1.0-2.0, respectively, by using a distiller.

Description

Eco-friendly sterilizer}

The present invention relates to an eco-friendly fungicide, and more particularly, to a fungicide containing distilled water obtained by distilling ginkgo leaves, bamboo leaves, mugwort leaves, mosquito leaves, green tea leaves, and pine needles.

Most disinfectants have developed and used chemical disinfectants, but these disinfectants may have side effects on the skin or human body.

Accordingly, there is a growing demand to develop a natural disinfectant that is harmless to the human body and has an eco-friendly and powerful sterilizing effect.

Recently, a humidifier disinfectant (cleaning agent) has caused lung injury syndrome (symptoms such as airway damage, shortness of breath, cough, and rapid lung damage (fibrosis)), which has become a social problem. Products are gaining popularity.

Accordingly, the present inventors developed an eco-friendly detergent by manufacturing these herbal plants into white liquid through distillation instead of general extraction in order to maximize the effect of the sterilizing power and have excellent sterilization power among herbal plants that have natural sterilization power. Was completed.

Korean Patent Registration No. 10-1527513 Korean Patent Registration No. 10-1518970

An object of the present invention is a fungicide containing distilled water obtained by distilling ginkgo leaves, bamboo leaves, mugwort leaves, mosquito leaves, green tea leaves and pine needles, which are harmless to the human body and have an eco-friendly and powerful sterilization effect, It is to provide a method for producing the same, and a use of the fungicide for sterilization of Escherichia coli or Pseudomonas aeruginosa.

In order to achieve the above object, the present invention is a gingko leaf, bamboo leaf, Swallowtail leaf, mosset leaf, green tea leaf, and pine needles, respectively, 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0 It provides a natural disinfectant containing a distillate obtained by distilling the mixture mixed in a ratio of.

In addition, the present invention

i) Mixture of gingko leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves and pine needles in the ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively, in a still Injecting the inside and heating water in the still to generate steam;

ii) Antibacterial contained in the ginkgo leaf, bamboo leaf, mugwort leaf, moset leaf, green tea leaf and pine needle by passing the water vapor generated in the still through the mixture of ginkgo leaf, bamboo leaf, mugwort leaf, woolen leaf, green tea leaf, and pine needle Or dissolving the bactericidal component in water vapor;

iii) cooling the water vapor absorbed by the antibacterial or bactericidal component through a cold water cooling tube; And

iv) extracting the distillate in which the antibacterial or sterilizing component is absorbed through the cooling process of the steam.

In addition, the present invention provides a method of sterilizing air, humidifier or applied water by spraying or adding the natural disinfectant according to the present invention to air, humidifier or applied water.

The present invention is harmless to the human body, environmentally friendly, and has a strong antibacterial effect against Escherichia coli, pneumococcal and Pseudomonas aeruginosa.

1 is a diagram showing a process of distilling ginkgo leaves, bamboo leaves, mugwort leaves, mosquito leaves, green tea leaves, and pine needles according to the present invention using a still.
Figure 2 is a pneumococcal (Klebsiella) of the natural fungicide according to the present invention pneumoniae ) and Pseudomonas aeruginosa ( Pseudomonas aeruginosa ).
Figure 3 is a natural fungicide according to the present invention pneumococcal bacteria ( Klebsiella pneumoniae ) This is a photograph showing the bactericidal effect on ATCC 4352.
Figure 4 is Pseudomonas aeruginosa of the natural fungicide according to the present invention aeruginosa ) This is a photograph showing the bactericidal effect on ATCC 27853.

Hereinafter, the present invention will be described in detail.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.

The present invention is a weight ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively. It provides a natural disinfectant containing a distillate obtained by distilling the mixture mixed with.

It is preferable to use after washing the ginkgo leaves, bamboo leaves, mugwort swallowtail leaves (serious mugwort leaves), mosquito leaves, green tea leaves, and pine needles after washing.

It is preferable to mix the gingko leaves, bamboo leaves, swallowtail mugwort leaves (Sorris wormwood leaves), mosquito leaves, green tea leaves, and pine needles in a weight ratio of 2:1.5:2:1.5:2:1.7, respectively.

The distillate is

i) adding a mixture of ginkgo leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves, and pine needles in an appropriate ratio into a still, and heating water in the still to generate water vapor;

ii) Antibacterial contained in the ginkgo leaf, bamboo leaf, mugwort leaf, moset leaf, green tea leaf and pine needle by passing the water vapor generated in the still through the mixture of ginkgo leaf, bamboo leaf, mugwort leaf, woolen leaf, green tea leaf, and pine needle Or dissolving the bactericidal component in water vapor;

iii) cooling the water vapor absorbed by the antibacterial or bactericidal component through a cold water cooling tube; And

iv) extracting the distillate from which the antibacterial or sterilizing component has been absorbed through the cooling process of the steam.

Specifically, the ginkgo leaves, bamboo leaves, mugwort leaves, mosquito leaves, green tea leaves, and pine needles are put into a still, and water in the still is heated to generate water vapor. At this time, it is preferable to perform the distillation process by adding 10 to 40 Kg to the leaves based on 100 Kg of water. If there is too little water, water vapor cannot properly absorb the antibacterial component when permeating the leaves. On the contrary, if there is too much water, the antimicrobial function is degraded because the amount of antibacterial component absorbed is too small compared to the final extracted distilled water. Therefore, it is important to control the weight ratio of the water and leaves. Then, the water vapor generated in the still is passed through the leaves, so that the antibacterial and bactericidal components contained in the leaves are dissolved in the water vapor. As the water vapor passes through the leaves, it absorbs the antibacterial and bactericidal components inherent in the leaves. Then, a process of cooling the water vapor absorbing the antibacterial and bactericidal components of the leaves is performed. At this time, the cooling process in the distiller is performed while water vapor passes through a spiral aluminum tube formed on the top of the distiller, and cooling may be accelerated by selectively operating a cooling fan at the bottom thereof. Then, a process of extracting distilled water absorbing the antibacterial and bactericidal components of the leaves is performed through the cooling process of steam. Distilled water extracted through the above process is used as a natural disinfectant.

The distillate may be used as a distillate mixture obtained by mixing the distillates obtained by distilling gingko leaves, bamboo leaves, serrata leaves (sorris wormwood leaves), mosset leaves, green tea leaves, and pine needles, respectively, through the distillation method.

The distillate is the mixture of step i) in the distillation method, respectively, 1.5 ~ 2.5: 1.0 ~ 2.0: 1.5 ~ 2.5: 1.0 ~ 2.0: 1.5 ~ It is also possible to use the hot-water extract obtained by hot-water extraction of the mixture mixed in a ratio of 2.5: 1.0 to 2.0. That is, first, ginkgo leaves, bamboo leaves, mugwort leaves, mosquito leaves, green tea leaves, and pine needles are added to water and heated at 70 to 110°C to distill the extracted extract to prepare a distillate.

The disinfectant is preferably an air disinfectant, a humidifier disinfectant, or a drinking water disinfectant.

In addition, the present invention

i) Mixture of gingko leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves and pine needles in the ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively, in a still Injecting the inside, heating water in the still to generate steam;

ii) Antibacterial contained in the ginkgo leaf, bamboo leaf, mugwort leaf, moset leaf, green tea leaf and pine needle by passing the water vapor generated in the still through the mixture of ginkgo leaf, bamboo leaf, mugwort leaf, woolen leaf, green tea leaf, and pine needle Or dissolving the bactericidal component in water vapor;

iii) cooling the water vapor absorbed by the antibacterial or bactericidal component through a cold water cooling tube; And

iv) extracting the distillate in which the antibacterial or sterilizing component is absorbed through the cooling process of the steam.

In the above manufacturing method, in step i), it is preferable to mix ginkgo leaves, bamboo leaves, mugwort swallowtail leaves, mosquito leaves, green tea leaves, and pine needles in a weight ratio of 2: 1.5: 2: 1.5: 2: 1.7, respectively. Do.

In the above manufacturing method, in step i), the mixture is 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0, respectively. It is also possible to use the hot-water extract obtained by hot-water extraction of the mixture mixed in a ratio of ~ 2.0.

In addition, the present invention provides a method of sterilizing air, humidifier or applied water by spraying or adding the natural disinfectant according to the present invention to air, humidifier or applied water.

The natural fungicide according to the present invention is Escherichia coli ), Pneumococcus ( Klebsiella pneumoniae ) and Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) has a remarkably excellent antibacterial effect, so it can be usefully used for antibacterial and sterilization of air, humidifiers or applied water.

Hereinafter, the present invention will be described in more detail through examples and experimental examples. These Examples and Experimental Examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these Examples and Experimental Examples. will be.

< Example 1> Preparation of natural fungicide

After washing and drying gingko leaves, bamboo leaves, mugwort Swallow leaves, mosquito leaves, green tea leaves, and pine needles, they were added to the inside of the still at a weight ratio of 2:1.5:2:1.5:2:1.7, respectively. 3 kg of total leaves were added to the still with 10 kg of water. The water vapor generated through the still was passed through the leaves, and the water vapor in which the leaf components were dissolved was cooled while the water vapor passed through the spiral aluminum tube formed in the upper layer of the still, and a colorless supernatant was obtained from the cooled distillate.

< Comparative example 1> Preparation of distilled water of ginkgo leaves

In the manufacturing method of <Example 1>, 3 kg of water based on 10 kg of water was added to the ginkgo leaf distillate to obtain a ginkgo leaf distillate.

< Comparative example 2> Preparation of distilled bamboo leaf water

In the manufacturing method of <Example 1>, 3 kg of water based on 10 kg of water was added to a still bamboo leaf to obtain a ginkgo leaf distillate.

< Comparative example 3> Green tea leaves Preparation of distilled water

In the manufacturing method of <Example 1>, 3 kg of water based on 10 kg of water was added to only green tea leaves to obtain a ginkgo leaf distillate.

< Comparative example 4> Preparation of distilled pine needles

In the manufacturing method of <Example 1>, 3 kg of water based on 10 kg of water was added to the still pine needles to obtain a ginkgo leaf distillate.

< Comparative example 5> Manufacture of distilled water of ginkgo leaves and bamboo leaves

In the manufacturing method of <Example 1>, 2 kg and 1 kg of ginkgo leaf and bamboo leaf were respectively added to a still with 10 kg of water to obtain a distillate of ginkgo leaf and bamboo leaf.

< Comparative example 6> Ginkgo leaves, Green tea leaves And production of distilled pine needles

In the manufacturing method of <Example 1>, a total of 3 kg of ginkgo leaves, green tea leaves, and pine needles were added to a still in a total of 1 kg each based on 10 kg of water to obtain a distillate of ginkgo leaves and bamboo leaves.

< Comparative example 7> Ginkgo leaves, bamboo leaves, Green tea leaves And production of distilled pine needles

In the manufacturing method of <Example 1>, ginkgo leaves, bamboo leaves, green tea leaves, and pine needles were added to a still with 1 kg, 0.5 kg, 1 kg and 0.5 kg of water based on 10 kg of water, respectively, and ginkgo leaves, bamboo leaves, and green tea. Distillates of leaves and pine needles were obtained.

< Experimental example 1> Check the antibacterial effect by direct treatment

The antibacterial effect of the natural fungicide according to the present invention against Escherichia coli and Pseudomonas aeruginosa was confirmed by comparing with comparative examples using only some components.

Escherichia coli ) (ATCC 8739) and Pseudomonas aeruginosa (ATCC 9027) were cultured at 30° C. in YPD medium for 12 hours. Their initial concentration was 10 ⁷ CFU/ml. This experiment was conducted under dark treatment to remove photoactivation. The cultured strain was diluted in a ratio of 1:100 in a medium containing each of the samples according to <Example 1> to <Comparative Example 7> and cultured. The concentrations of the samples were diluted to 1, 1/10, 1/50, and 1/100, respectively, and tested. Incubation for 12 hours to observe the growth of the bacteria.

The results are shown in Table 1 below. As shown in Table 1, Example 1 has an excellent sterilizing effect against Escherichia coli and Pseudomonas aeruginosa, and Comparative Examples 1 to 7 using only some leaves of the leaves of Example 1 have some sterilization effects, but are significantly inferior to Example 1. Confirmed.

sample microbe density
One density
1/10 density
1/50 density
1/100 Example 1 Escherichia coli - - - - Pseudomonas aeruginosa - - - - Comparative Example 1 Escherichia coli - growth growth growth Pseudomonas aeruginosa - growth growth growth Comparative Example 2 Escherichia coli growth growth growth growth Pseudomonas aeruginosa - growth growth growth Comparative Example 3 Escherichia coli - - growth growth Pseudomonas aeruginosa - growth growth growth Comparative Example 4 Escherichia coli - growth growth growth Pseudomonas aeruginosa - growth growth growth Comparative Example 5 Escherichia coli - - growth growth Pseudomonas aeruginosa - - growth growth Comparative Example 6 Escherichia coli - - growth growth Pseudomonas aeruginosa - - growth growth Comparative Example 7 Escherichia coli - - growth growth Pseudomonas aeruginosa - - growth growth

< Experimental example 2> Check the antibacterial effect by air spray

In order to confirm the sterilizing effect of the natural fungicide according to the present invention against pneumococcal and Pseudomonas aeruginosa, it was confirmed by requesting the Korea Institute of Standards and Testing.

Specifically, pneumococcal ( Klebsiella a pneumoniae) (ATCC 4352) and Pseudomonas aeruginosa (pseudomonas aeruginosa) (ATCC 27853) was cultured. The concentrations of these inoculum solutions were 3.3 × 10 ⁴ CFU/mL and 1.3 × 10 ⁴ CFU/mL, respectively. 10 ml of the sample of Example 1 was sprayed into the air, the test temperature was 35°C, and the test time was 1 hour. As a control group, physiological saline was sprayed in the same amount and compared.

The results are shown in Table 2 and FIGS. 2 to 4 below. As shown in Table 2 and FIG. 2, Example 1 was sterilized by 98.1% against pneumococcus and 96.9% against Pseudomonas aeruginosa.

sample microbe Bacteriostatic reduction rate (%) Example 1 Klebsiella pneumoniae
ATCC 4352 98.1 Example 1 pseudomonas aeruginosa
ATCC 27853 96.9

As described above, specific parts of the present invention have been described in detail, and it is obvious that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereby. something to do. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

Distillation obtained by distilling a mixture of gingko leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves, and pine needles in the ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively Natural fungicide containing liquid.
The method of claim 1,
The distillate is
i) Mixture of gingko leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves, and pine needles in the ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively, in a still Injecting the inside, heating water in the still to generate steam;
ii) Antibacterial contained in the ginkgo leaf, bamboo leaf, mugwort leaf, moset leaf, green tea leaf and pine needle by passing the water vapor generated in the still through the mixture of ginkgo leaf, bamboo leaf, mugwort leaf, woolen leaf, green tea leaf, and pine needle Or dissolving the bactericidal component in water vapor;
iii) cooling the water vapor absorbed by the antibacterial or bactericidal component through a cold water cooling tube; And
iv) extracting the distillate in which the antibacterial or sterilizing component is absorbed through the cooling process of the steam.
The method of claim 2,
The mixture is a mixture of gingko leaves, bamboo leaves, mugwort leaves, seedlings leaves, green tea leaves, and pine needles in a ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively. Natural fungicide, characterized in that using the hot water extract obtained by hot water extraction.
The method of claim 1,
The disinfectant is an air disinfectant, a humidifier disinfectant, or a drinking water disinfectant.
i) Mixture of gingko leaves, bamboo leaves, mugwort leaves, mosset leaves, green tea leaves, and pine needles in the ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively, in a still Injecting the inside, heating water in the still to generate steam;
ii) Antibacterial contained in the ginkgo leaf, bamboo leaf, mugwort leaf, moset leaf, green tea leaf and pine needle by passing the water vapor generated in the still through the mixture of ginkgo leaf, bamboo leaf, mugwort leaf, woolen leaf, green tea leaf, and pine needle Or dissolving the bactericidal component in water vapor;
iii) cooling the water vapor absorbed by the antibacterial or bactericidal component through a cold water cooling tube; And
iv) extracting the distillate in which the antibacterial or bactericidal component is absorbed through the cooling process of the steam.
The method of claim 5,
The mixture of step i) contains gingko leaves, bamboo leaves, mugwort leaves, seedlings leaves, green tea leaves, and pine needles in a ratio of 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0: 1.5 to 2.5: 1.0 to 2.0, respectively. A method for producing a natural fungicide, characterized in that it is a hot water extract obtained by extracting the mixed mixture with hot water.

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