KR20160004878A - extracts of Chamaecyparis obutsa, method for preparing thereof, and composition comprising the same - Google Patents

Abstract

The present invention relates to a producing method of a cypress extract and various compositions using the cypress extract. The producing method of the cypress extract comprises the following steps: selecting a raw material from 10-20-year-old cypresses; steaming the selected raw material with a copper plate; extracting an extract by cooling and condensing steam generated by the steaming step; separating the extract into cypress oil and cypress water; and ripening each of the cypress oil and cypress water at a low temperature of 10°C or less for 30 days or longer. The cypress extract of the present invention contains different components from a previous produced product by adding a copper (Cu) component when producing the cypress extract, contains abundant minerals, and has equal to or more than 5% of a terpene-based compound which is a major component of phytoncide. The cypress extract according to the present invention contains components providing a skin smoothing effect, an allergy relieving effect, a virus infection preventing effect, etc. In addition, the cypress extract providing a cool mint scent is effective against pains, infections, and headaches and helpful to regain mental and physical stability.

Description

The extract of Chamaecyparis obutsa, a method for preparation thereof, and a composition comprising the same,

FIELD OF THE INVENTION [0002] The present invention relates to an extract of Eucalyptus thunbergii, a method for producing the Eucalyptus extract,

The refreshing air we feel when we go to the forests relieves stress and makes us feel relaxed. The material that affects this is phytoncide.

Phytoncide (phytoncide) is a mixture of phyton (plant), which means plant in Greek, and cide (killer), which means sterilizing power. The directional antibiotics that all plants emit in the air or in the ground to defend themselves are collectively called phytoncide.

Phytoncide's attention is attracted to various pests, germs, fungi, and bacteria that threaten him, but he acts as a killer, but works well for humans. Phytoncide is a natural substance that is not a chemical synthetic substance. It is rapidly absorbed by human body and selectively sterilizes microorganisms harmful to humans. Therefore, phytoncide is known to have a number of functions such as antimicrobial action, deodorizing action, soothing action, and stress relieving action. This phytoncide is absorbed in our body, it works on the central nervous system such as extension of the sleeping time, blood pressure control function, and so on.

These phytoncides are extracted especially from cottonwood, shrubs, etc., and the main component of phytoncide is terpene-type compound, which is known to have a unique directionality.

Currently, various products using phytoncide, which is an extract of perilla, have been marketed, but most of them are mostly mixed with organic materials or only a part of the extract.

Therefore, it is not environmentally safe to use for various purposes, and the effect of the extract itself can not be exhibited properly.

Korean Patent Publication No. 2004-0089942

The inventors of the present invention have studied a method capable of exerting the effect of a cotton-on-white extract itself, without mixing the cotton-brown extract having excellent effects on various surfaces with an organic compound harmful to human and environment as described above.

Accordingly, it is an object of the present invention to provide a novel method for producing a white flour extract which can exert its own effects with the white flour extract itself.

It is another object of the present invention to provide a composition for various uses, which comprises a simplex oil or a mannitol alone, or a mixture thereof, in the above-described whitening extract.

The method for preparing the white flour extract according to the present invention comprises the steps of selecting raw cotton materials for ten to twenty years, boiling the selected raw cotton materials with copper, cooling and condensing the steam generated in the boiling step, , Separating the extracted extract into a white oil / white water, and subjecting the separated white oil / white water extract to low temperature aging at 10 ° C or lower for 30 days or more.

In addition, the present invention can provide a composition comprising a whitening oil among the whitening extracts prepared by the above method.

In addition, the present invention can provide a composition comprising the elixir water among the elixir extracts prepared by the above method.

In addition, the present invention provides a composition comprising a mixture of a mannitol oil and a mannitol in a mannitol extract prepared by the above method, wherein the composition comprises 0.01 to 20% of mannitol oil relative to the volume of mannitol .

The composition may be any one selected from the group consisting of cosmetics, detergents, beverages, fragrances, deodorants, repellents, antibacterial agents, and humidifying fluids.

The present invention relates to a method for producing a cottonseed extract, which comprises adding a copper (Cu) component to produce a white flake extract, having a different component from that of a previously prepared product and having a rich mineral content and a content of a terpene- Or more.

The present invention relates to a composition for preventing or treating skin sores, which is effective for relieving skin allergies and preventing viral infections, and is effective in relieving analgesic, anti-inflammatory and mint flavor, relieving headaches, and relieving stress to be.

Therefore, the composition of the present invention can be applied to cosmetics, detergents, beverages, fragrances, deodorants, insect repellents, antimicrobial agents, and humidifying liquids.

FIG. 1 is a schematic diagram of an extractor used in a process for producing a white flour extract according to the present invention,
2 and 3 are graphs showing the results of volatile components (GC-MS) of the water-repellent water and the monofilament oil prepared according to the present invention,
Figs. 4 and 5 are photographs of skin changes before and after using the whitening essence prepared according to the present invention.

Hereinafter, the present invention will be described in more detail.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a," "an," and "the" include singular forms unless the context clearly dictates otherwise. Also, " comprise "and / or" comprising "when used herein should be interpreted as specifying the presence of stated shapes, numbers, steps, operations, elements, elements, and / And does not preclude the presence or addition of one or more other features, integers, operations, elements, elements, and / or groups.

The present invention provides a novel method for producing a white flour extract which can exert its own effects with only a white flour extract, and a composition for various uses including the white flour extract.

The term "cotton wool extract" of the present invention means that the white cotton wool extract is extracted by distillation method using a white cotton of about 10 to 20 years, and the white cotton wool extract includes a white cotton oil, a white cotton wool, and a mixture thereof.

The method of the present invention comprises the steps of selecting a raw cotton material for ten to twenty years, boiling the selected raw cotton material with a copper plate, cooling, condensing and extracting steam generated in the boiling step, Separating the extracted extract into a white oil / white water, and subjecting the separated white oil / white water extract to low-temperature aging at 10 ° C or lower for 30 days or more.

Specifically, it can be produced through the process shown in Fig.

First, in the present invention, the raw materials for untwisted wood are selected, and the untwisted wood raw materials can be selected from the untwisted tree branches and the untwisted leaves obtained from the untwisted trees of about 10 to 20 years distributed in the clean area of Korea such as Goheung, Jeollanamdo. As in the present invention, when the raw material of untreated white wood is used for 10 to 20 years, the best aroma and refreshing feeling can be felt.

In addition, the raw material for the untreated wood according to the present invention may be any of the untwisted tree branches and the untwisted leaves, but it is more preferable to use the untwisted leaves for the production of the highly efficient extract. It is preferable that the sampled monofilament raw material is cleaned and then crushed to a size of about 2 to 3 cm.

In the second step, the obtained raw white wood material is put into a vessel such as a hot pot set in a facility equipped with a heating heater and a thermometer, and is subjected to a steaming process. In the present invention, it is particularly preferable to perform the above-described process in which the selected unvoiced wood raw material is added under the addition of copper plates. Herein, the term " addition of copper plate " means that a copper plate made of copper (Cu) is placed in the container, and the copper plate raw material is put on the copper plate and steamed or the inside of the container is coated with copper, Quot; means to include a method of putting a raw material of unconverted wood into a container made of a material such as rice, and to steep the raw material of the unfinished wood and the copper in substantially contact with each other. Accordingly, the term 'with copper plate' in the present invention means any form in which copper is added in the container into which the raw cotton material is injected.

In the present invention, when the raw material of the unfinished wood is subjected to the addition of the copper plate during the steaming process, various toxins (such as ozone) which may remain in the unfinished wood raw material due to contaminated rain and dust even when the raw untreated raw material is cleaned It has a neutralizing effect. Therefore, it is possible not only to obtain a higher purity cottonseed extract, but also to obtain an environmentally friendly cottonseed extract, thereby maximizing its effect when it is used for various purposes.

Preferably, the boiling process is carried out by adding 30 to 50% of ground water to the plant, and heating the plant to a temperature of about 120 to 150 ° C for about 5 to 7 hours.

Also, it is preferable that the steaming process of the present invention does not directly contact the water and the untreated wood raw material, but steam only the water vapor formed by heating the added ground water. This is to prevent dilution of the effective ingredient concentration in the final production of the cotton-on-white extract because the various effective ingredients contained in the raw cotton material can be released into water when the raw cotton material is contacted with water.

Therefore, as shown in Fig. 1, it is preferable to substantially add the ground water to the outside of the steamer where the raw cotton material is placed and heat it.

The third step is to cool and condense the steam generated in the process of steaming the second pre-cooked raw material to extract it. In this step, a variety of various components including the white oil component contained in the raw cotton material are volatilized along with water vapor generated by heating and sent to the cooler. The water vapor containing the oil component passing through the cooler is condensed through the heat exchanger inside the cooler, and is stored as condensed water mixed with the white oil and the white water through the condenser.

The fourth step is a step of separating the stored condensed water through the extraction step into the untreated oil / untreated water. The method of separating the condensed water into the plain oil and the untreated water is not limited to a specific method, and a known method such as specific gravity difference, centrifugal separation, or the like can be used.

Finally, the separated monoaxial oil and the extract of each of the maleic anhydrase are subjected to a low temperature aging at 10 DEG C or less, preferably 0 to 8 DEG C for 30 days or more, preferably 30 days to 90 days. Through the low-temperature aging process, it is possible to remove harmful components that may remain in the monoaxial oil and the monoaxial extract.

The thus-produced white flour extract (white oil and white water) according to the present invention has a characteristic that the content of a terpene-based compound as a main component of the phyton cust is 5% or more.

In addition, the present invention can provide a composition comprising the whitening oil of the above-described whitening extract. That is, various compositions can be obtained by using only a simple oil component without containing any organic compound. The composition may be used in cosmetics, detergents, beverages, fragrances, deodorants, insect repellents, antimicrobial agents, , And may contain 100 wt% of the monoaxial oil component alone, or may be added to the basic composition of the composition in an amount of 0.1 to 20 vol% in the range of 0.1 to 20 vol%.

In addition, the present invention can provide a composition comprising the elixir water among the elixir extracts prepared by the above method. That is, various compositions can be obtained by using only the simplex component. That is, various compositions can be obtained by using only the untreated water component without including any organic compound. The composition may be any one selected from cosmetics, cleaning agents, beverages, fragrances, deodorants, insect repellents, antimicrobial agents, and humidifying liquids, and may contain 100 wt% By mass of a water-repellent water component in a range of 0.1 to 20% by volume.

In addition, the present invention provides a composition comprising a mixture of a whitening oil and a mannitol, wherein the composition comprises 0.01 to 20% of mannitol oil relative to the volume of mannitol. That is, according to the present invention, it has been observed that the effect can be maximized if it is separated into the whitening oil and the untranslated water and then mixed again with an appropriate amount ratio. If the content of the white oil in the composition is more than 20% by volume, the flavor of the white oil is too strong to adversely affect the user, which is not preferable.

The composition may be used in any one of a cosmetic, a cleaning agent, a beverage, a fragrance, a deodorant, a repellent, an antibacterial agent, and a humidifying solution.

When the composition according to the present invention is a cosmetic composition, it can be prepared into any of the formulations conventionally produced in the art. For example, it can be used as a softening lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, , A cleansing water, a pack, a spray, or a powder.

In addition, when the composition according to the present invention is used as a fragrance or a deodorant, it can be used in various forms such as an air purifier, an air conditioner, an air conditioner, a dehumidifier, an automobile air conditioner, an air filter, Can be used.

In addition, the composition according to the present invention may be prepared by varying the concentration thereof according to a desired form such as spray, gel, lotion, liquid phase, and the like.

Hereinafter, preferred embodiments of the present invention will be described in detail. The following examples are intended to illustrate the present invention, but the scope of the present invention should not be construed as being limited by these examples. In the following examples, specific compounds are exemplified. However, it is apparent to those skilled in the art that equivalents of these compounds can be used in similar amounts.

Example  : Manufacture of cottonseed extract

The leaves and stems, which were obtained from the cottonwood between 10 and 20 years old in Goheung, Jeollanam - do, were thoroughly cleaned and crushed to 2 ~ 3cm size to obtain the raw cottonwood. The raw material was placed in a hot pot (manufactured by copper plate) installed in a facility equipped with a heating heater and a thermometer, and 35% of ground water with respect to the volume of the raw white wood raw material was placed on the outside of the cabinet of the above equipment, I've been out for a while.

The water vapor generated in the process of steaming the raw materials was cooled through a cooler, and the extracts of Cryptomeria japonica, which had been condensed through a condenser, were separated into mono-oleaginous oil and manure water using specific gravity difference.

The separated monoaxial oil and monoclinic water were aged at 5 캜 for 30 days, respectively, to obtain a final Clostridium perfringens extract.

Example  2: Production of cottonseed extract

The raw cotton material was obtained in the same manner as in Example 1, and then a flat copper plate was placed in a hot pot, and the raw white rice material was placed thereon, followed by steaming for 5 hours at a temperature of about 150 ° C. Extraction and separation of white oil / white water were carried out in the same manner as in Example 1, and the separated white oil and white water were aged at 8 ° C for 60 days, respectively, to obtain a final white tea extract.

Experimental Example  1: Cottonseed extract (cottonseed oil / Number of Writes ) Component analysis

Volatile components and minerals contents were analyzed by GC-MS, IC, HPLC and pH meter according to the following Tables 1 and 2, respectively.

The results of measurement of the volatile components (GC-MS) of the untreated water and the untreated oil are shown in Tables 2 to 3 and Tables 3 and 4, respectively, and the results of measurement of the mineral content (IC) are shown in Table 5 below.

Model GC 2010, GC-MS (QP-2010 plus) column Rtx-5 (60 m x 0.25 mm x 0.25 m) Carrier gas Helium (1 ml / min) Condition injector 250 ° C, detector 330 ° C, oven temperature 250 ° C Mass data Willy 8th ed.

ion Anion Cation Cl ^- Na ⁺ , K ⁺ , Ca ^{2 +} Model ICS-1000 (DIONEX) ICS-1000 (DIONEX) Standard solution Dionex Dionex column Ionpac AG1 Ionpac CG 12A Eluent 0.5 mM Na ₂ CO ₂ ,
0.3mM NaHCO ₂ 0.5 mM Na ₂ CO ₂ ,
0.3mM NaHCO ₂ Flow rate 1.5 mL / min 1.5 mL / min Measure conductivity conductivity

No Rt ^(a) area
(%) Ingredients Molecular formula
(Molecular Weight) efficacy pH 2 11.1 1.6 3- (Z) -Hexenol C ₆ H ₁₂ 0
(100) Horsetail foot drain Main ingredient, tea leaves Fragrance ingredient 4.8 3 19.9 1.3 alpha-terpinolen C ₁₀ H ₁₆
(136) Blocks infection with human viruses, relieves blood in the nose and improves concentration 4 20.5 1.1 3-Cyclohepten-1-ol C ₁₀ H ₁₈ 0
(154) Aromatics, skin soothing 5 21.7 57.5 1-Methane-4-ol C ₁₀ H ₁₈ 0
(154) Analgesic, anti-inflammatory, directional ingredients of peppermint, refreshing fragrance 6 22.1 5.9 (+) - [alpha] -Terpineol C ₁₀ H ₁₈ 0
(154) Main ingredient of Caju foot oil (tiger ointment), headache relief, mental and physical stability 7 24.9 5.1 Acectic acid, bornylester C ₁₂ H ₂₀ 0 ₂
(196) Volatile component of forest bath, perfume ingredient 8 26.5 3.3 Acectic acid, bornylester C ₁₂ H ₂₀ 0 ₂
(196) Volatile component of forest bath, perfume ingredient 9 31.9 2.0 Cyclohexane C ₁₅ H ₂₆ 0
(222) Skin soothing effect 10 34.4 1.1 α-Eudesmol C ₁₅ H ₂₆ 0
(222) Antihistamine action to alleviate allergic asthma 11 40.6 4.5 (+) - Hibaene C ₂₀ H ₃₂
(272) Fragrance ingredients such as cypress Rt ^(a) is the time at which the component is extracted.

1-methene-4-ol (C ₁₀ H ₁₈ 0), which is a component that imparts a refreshing smell due to analgesic, anti-inflammatory and peppermint fractions, is included in the white water produced according to the present invention. Was contained in large quantities and was found to contain ingredients that showed effects such as skin soothing, allergy relief, and blocking of virus infection.

In addition, the major component of the phytoncide contained more than 7% (based on the area basis) of the terpene compound, thus confirming that it has a refreshing aroma of analgesic, anti-inflammatory and peppermint, relieves headache, and has an effect on mental and physical stability.

No Rt ^(a) area
(%) Ingredients Molecular formula
(Molecular Weight) efficacy pH One 13.7 3.1 2.7-Dimethyl-3-octene C ₁₀ H ₁₆
(136) Fragrance component of tea leaves 3.4
2 11.1 1.6 Linolenic acid methyl ester C ₁₉ H ₃₂ 0 ₂
(292) It is an omega-3 fatty acid, which contains DHA, EPA phone, thrombotic disease, brain myocardial infarction, inflammation, allergy, cancer, 3 15.6 6.9 7-Bicycloheptane C ₁₀ H ₁₆
(136) Used as a nonpolar solvent in the manufacture of functional foods 4 16.5 2.9 alpha-terpinolen C ₁₀ H ₁₆
(136) Preventing infection with human viruses 5 16.9 9.4 Linolenic acid methyl ester C ₁₉ H ₃₂ 0 ₂
(292) Components such as No 2 6 17.9 6.5 2,7-Dimethyl-3-octen-5-yne C ₁₀ H ₁₆
(136) Fragrance component of tea leaves 7 18.8 2.8 alpha-terpinolen C ₁₀ H ₁₆
(136) Blocks infection with human viruses, relieves blood in the nose and improves concentration 8 21.7 5.9 cis-Carvotanacetol C ₁₀ H ₁₈
(138) Volatile flavor component 9 24.9 10.3 Dihydrocarveol C ₁₀ H ₁₈
(138) Used on skin Sterilization, preservation, antifungal, antiviral effect 10 26.7 11.6 Linolenic acid methyl ester C ₁₉ H ₃₂ 0 ₂
(292) Components such as No 2 11 31.8 5.0 2-Propanol C ₁₅ H ₂₆ 0
(222) Flower fragrance Aliphatic saturated alcohol 12 40.6 3.7 (+) - Beyerene C ₂₀ H ₃₂
(272) Phiton chedd Rt ^(a) is the time at which the component is extracted.

In addition, referring to the results of Table 4, it can be seen that the soft white oil produced according to the present invention is an omega 3 fatty acid, which is effective in reducing DHA, EPA phone, thrombotic disease, cerebral myocardial infarction, inflammation, allergy, Methyl ester (C ₁₉ H ₃₂ 0 _{2 )} component, and was found to contain components exhibiting effects such as antiviral, antifungal, skin soothing, allergy relief, and viral infection prevention.

Also, it was confirmed that the major component of the phytoncide contains more than 5% (area basis) of the terpene compound, and thus it can act as a main component of the vascular disease, blocking of the infection of the body virus, relieving headache, and mental and physical stability.

(Unit: mg / L (ppm) Linseed oil Number of Writes No. Drinking water ⁽¹⁾ Anion Cl ^- 147 150 11.5 <150 Cation Na ⁺ 100 60 5.6 <200 K ⁺ 2.4 2.3 67.9 <10 Ca ⁺² 5.8 6.3 63.8 <300 (1) Concentration of Ca and Mg for potable water: Annual water 0 ~ 60, Chond Water 60 ~ 120, Water 120 ~ 180

Referring to Table 5, it can be seen that the contents of minerals including anions and cations satisfy the conditions of the drinking water in the whitening oil and the manure water produced according to the present invention.

Accordingly, it has been confirmed that the white oil and the white water according to the present invention can be used as drinking water.

Example  3: Manufacture of fragrance using the extract

A perfume composition containing 100 wt% of the soft white oil component prepared in Example 1 was prepared.

Experimental Example  2: Detection of harmful components of fragrance

The perfume composition prepared in Example 3 was dried for 72 hours at a relative humidity of 20 ± 2 ° C and 50 ± 5% After use, it was determined whether or not harmful components were detected. The results are shown in Table 6 below.

standard Example 3 judgment Harmful component Methyl alcohol (%) 0.2 or less Non-detection pass Formaldehyde (mg / L) 25 or less 22 pass

Referring to Table 6, it was confirmed that methylalcohol was not detected when using the white wool extract prepared according to the present invention (whitening oil) as a fragrance, and formaldehyde was also detected within the usable range. Therefore, it can be seen that the extract of the white flakes according to the present invention can be usefully used as a perfume composition.

Example  4 ~ 8: Preparation of Cotton White Essence with Cotton Leaf Extract

A spray-type skin care essence containing 0.1, 1, 5, 10, and 20% of the unctuous white oil components, respectively, as compared with the untreated water volume prepared in Example 1 was prepared.

Experimental Example  3: Stress Hormone Reduction in the Waxy Essence

In order to observe whether the extract of the white flour according to the present invention is effective for relieving stress, a 110 V current is first applied to the experimental rats for 0.03 second at intervals of 3 seconds for 1 minute to increase the stress by applying electrical stimulation to the cortisol The concentration was increased rapidly. Then, the albino essence prepared according to Example 4 was inhaled in the experimental rats, and the blood concentrations of other cortisol were measured at different times. The results are shown in Table 7 below.

For this experiment, a commercial product containing a cottonseed extract was used as a control.

The concentration of cortisol (ng / mL) Cortisol concentration reduction rate (%) Before stimulation After stimulation Enriched white essence after inhalation

4.7

73.8 Example 4 35.0 52.5 Example 5 32.8 55.5 Example 6 29.5 60.7 Example 7 30.1 55.1 Example 8 30.0 55.2 Control group 63.7 13.7

Referring to the results of Table 7 above, it was experimentally confirmed that the concentration of cortisol which causes stress is significantly reduced when using the white cotton oil mixed with the white cotton oil, which is the white cotton oil extract prepared according to the present invention, with the white cotton oil.

In addition, it has been confirmed that the antler extract according to the present invention has an excellent stress reduction effect as compared with existing commercial products. Therefore, it is expected that the use of the whitening essence according to the present invention will have an effect of calming the mind similar to the forest bathing in the actual coniferous forest and relieving the stress.

Experimental Example  4: Antimicrobial activity and antifungal activity measurement

To compare the bactericidal activity of the antimicrobial extracts against the antimicrobial activity of antimicrobial agents (antibiotics, antifungal agents) against the various bacteria, the medicament (control) and the whitening essence were separately treated in a culture medium containing the cultivated bacteria, And the results are shown in Table 8 below.

Type of germ Sterilization range (mm) ^* Germ characteristics Control ^** Example 4 5 6 7 8 Listeria 20 13 10 9 7 6 Food poisoning, meningitis, maternal heritage Staphylococcus aureus 28 16 13 12 10 11 Encephalitis, otitis media, cystitis, middle ear, various inflammation Always resistant
Staphylococcus 28 10 10 9 8 8 Aka Super bacteria, a bacterium resistant to bencomycin Legionella 10 10 9 8 7 6 Pneumonia, high fever, diarrhea. Transmission with humidifier cooling tower Candida 12 11 10 11 10 9 Female vaginitis, itching, thrush * The figures are the comparison of the sterilization power of each of the bacteria in the control group and in the Example white rice essence.
** Control group is antibiotic chloramphenicol, antifungal Terbinaline.

Referring to the results shown in Table 8, it was confirmed that the whitening essence mixed with the whitening oil, which is a whitening extract prepared according to the present invention, and the whitening water were excellent in antibacterial and antifungal properties. Accordingly, it can be confirmed that the skin whitening essence according to the present invention can be effectively used in the case of skin itching, skin allergies, or the like which responds to the above-mentioned germicidal characteristics.

Experimental Example  5: skin of the skin of essence allergy  Efficacy experiment

Experiments suffering from skin diseases for two years confirmed changes in the before and after the use of the enamel essence spray according to Example 6 several times a day for a week. The results are shown in FIGS. 4 and 5.

In Figure 4, before using the albinish essence, a considerably large skin problem occurred around the neck and itching was severe.

However, as shown in FIG. 5 after one week's use of the white currant essence according to the present invention, it can be seen that the skin troubles in the neck completely disappeared.

It has been confirmed that the present invention can be effectively applied to the skin soothing effect of the whitening extract and the treatment of atopy by optimizing the content of the whitening oil according to the present invention.

Claims (5)

A step of screening the raw white wood for 10 to 20 years,
A step of pouring the selected unleaved wood raw material under the addition of a copper plate,
Cooling, condensing and extracting steam generated in the boiling step,
Separating the extracted extract into a whitening oil / shortening water, and
Subjecting the separated monoaxial oil / monoaxel extract to low-temperature aging at 10 ° C or lower for 30 days or more.
A composition comprising a monoaxial oil extract prepared by the method of claim 1.

A composition comprising a maleic anhydrase extract prepared by the method of claim 1.

11. A composition comprising a cottonseed oil and a cottonseed mixture, prepared by the method of claim 1, wherein the composition comprises an oil component in an amount of from 0.01 to 20% by volume of the fluid. 5. The method according to any one of claims 2 to 4,
Wherein the composition is any one selected from cosmetics, detergents, beverages, fragrances, deodorants, insect repellents, antimicrobial agents, and humidifying fluids.

Images