KR102176712B1 - Antiseptic Composition and wet wipes comprising the same - Google Patents

Abstract

The present invention relates to a preservative composition and a wet tissue comprising the same, specifically (i) sodium benzoate; And (ii) at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; a preservative composition comprising, a wet tissue liquid containing the preservative composition, a wet tissue comprising the wet tissue liquid, the Preservative power of sodium benzoate comprising the step of impregnating a wet tissue preparation method and sodium benzoate with at least one selected from the group consisting of caprylyl glycol and dipropylene glycol It's about how to improve.
The preservative composition of the present invention induces initial microbial death and exhibits a stable microbial control effect, and thus can be usefully used in the development of antibacterial wipes.

Description

Antiseptic Composition and wet wipes comprising the same}

The present invention relates to a preservative composition and a wet tissue comprising the same, specifically (i) sodium benzoate; And (ii) at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; a preservative composition comprising, a wet tissue liquid containing the preservative composition, a wet tissue comprising the wet tissue liquid, the Preservative power of sodium benzoate comprising the step of impregnating a wet tissue preparation method and sodium benzoate with at least one selected from the group consisting of caprylyl glycol and dipropylene glycol It's about how to improve.

Wet wipes, which are constantly increasing in demand because of their convenience and ease of use, are generally composed of water and non-woven fabrics, and water and non-woven fabrics account for more than 99%. Water and non-woven fabrics contain many kinds of microorganisms, so there is a high possibility of exposure to contamination during the manufacture of wet tissues, and the use of strong preservatives is essential because contaminated wet tissues grown by microorganisms have a high risk of deterioration and deodorization of the product. . In particular, water is inherently susceptible to contamination by bulcholderia among microorganisms, and non-woven fabrics are inherently susceptible to contamination by bacillus among microorganisms, but conventional preservatives for wet tissues have a problem that the antibacterial activity against the two microorganisms decreases. , There is a need to develop a preservative composition capable of effectively and strongly killing the two microorganisms.

Currently, the most commonly used preservatives for wet tissues are quaternary ammoniums such as cetrimonium bromide, cetrimonium chloride, and cetylpyridinium chloride. However, quaternary ammonium-based preservatives have a lot of foam generation and thus require the use of a defoaming agent, and have disadvantages in that productivity is poor due to pop-up phenomenon due to foam, and there is a high risk of contamination and trouble in the manufacturing process.

Accordingly, sodium benzoate, which is widely used in foods, has been mainly used as a preservative in recent years, but sodium benzoate has a somewhat low preservative power, and as the related control technology is applied in a state that has not been established, bacteria detection issues frequently arise. have. In order to solve the low preservative power of sodium benzoate, the content of sodium benzoate is continuously increased and used, and recently, it is a problem because it is used in excess of 0.5%, which is the legal standard. As another solution, there is an attempt to increase the preservative power of sodium benzoate by adjusting the pH (Biotech Health Sci. 2016 August; 3(3): e35084.), but acidic conditions are effective in controlling microorganisms, but skin irritation is severe. Another solution is needed.

Under this background, the present inventors have made intensive research efforts to develop a material that can improve the antiseptic power of sodium benzoate, and as a result of adding caprylyl glycol and/or dipropylene glycol to sodium benzoate, By inducing initial microbial death and confirming that a stable microbial control effect was shown, the present invention was completed.

One object of the present invention is (i) sodium benzoate; And (ii) one or more selected from the group consisting of caprylyl glycol and dipropylene glycol; to provide a preservative composition comprising.

Another object of the present invention is to provide a wet tissue liquid containing the preservative composition.

Another object of the present invention is to provide a wet tissue comprising the liquid tissue for wet tissue.

Another object of the present invention is to provide a method for producing a wet tissue comprising the step of impregnating the wet tissue liquid.

Another object of the present invention is to provide a method for improving the antiseptic power of sodium benzoate, comprising the step of adding at least one selected from the group consisting of caprylyl glycol and dipropylene glycol to sodium benzoate. will be.

In order to achieve the above object, one aspect of the present invention is (i) sodium benzoate; And (ii) at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; provides a preservative composition comprising a.

The preservative composition of the present invention comprises (i) sodium benzoate; And (ii) one or more selected from the group consisting of caprylyl glycol and dipropylene glycol.

The preservative composition of the present invention may include sodium benzoate, caprylyl glycol, and dipropylene glycol.

The term of the present invention, "sodium benzoate (SB)" has a structure of the following formula 1, refers to a compound of the molecular formula C ₇ H ₆ O _{2 ·} Na. In general, it is used as a preservative for food or cosmetics, as a preservative for paint or as a corrosion inhibitor, and is especially used as a corrosion inhibitor, fragrance, and sterilization preservative in cosmetics.

[Formula 1]

The term of the present invention, "caprylyl glycol (CG)" has a structure of the following formula (2), refers to a compound of the molecular formula C ₈ H ₁₈ O ₂ . It is generally used as a hair conditioning agent and skin softening agent. Caprylyl Glycol is extracted from natural products and is hypoallergenic and shows high moisturizing power.

[Formula 2]

The term of the present invention, "dipropylene glycol (DPG)" has a structure of the following formula (3), refers to a compound of the molecular formula C ₆ H ₁₄ O ₃ . In general, it is used as a wetting agent, solvent, viscosity reducing agent, flavoring agent, etc. in cosmetics. In particular, the effect of improving the preservation power thereof is not known, and was first identified by the inventors.

[Formula 3]

As used herein, the term "antiseptic" refers to any substance used for the purpose of preventing changes in the product by inhibiting the growth of microorganisms contaminating the product from the outside. If it can suppress the proliferation of microorganisms and exhibit antibacterial activity, it is included in the range of preservatives of the present invention, specifically bacteria and fungi, more specifically Escherichia coli, Bacillus, Burkholderia, Pseudomonas aeruginosa (Pseudomonas aeruginosa), Staphylococcus aureus, Candida albicans, and black mold (Aspergillus niger) may inhibit the proliferation and exhibit antibacterial activity, but is not limited thereto.

The preservative composition of the present invention may be for wet tissue.

In a specific embodiment of the present invention, a wet tissue sample was prepared by impregnating the fabric with a chemical for wet tissue.

The preservative composition of the present invention is Escherichia coli, Bacillus, Burkholderia, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Black fungus. ) It may be one having antibacterial activity against at least one selected from the group consisting of.

The preservative composition of the present invention may be one having antibacterial activity against Bacillus or Burkholderia.

In a specific embodiment of the present invention, sodium benzoate of the present invention; And at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; when treated with a preservative composition comprising; Escherichia coli, Bacillus (Bacillus), Burkholderia, Pseudomonas aeruginosa (Pseudomonas aeruginosa) and Staphylococcus aureus (Pseudomonas aeruginosa) and Staphylococcus aureus (Staphylococcus aureus) five kinds of bacteria and Candida (Candida albicans) and black fungus (Aspergillus niger) of two kinds of fungi (Aspergillus niger) appeared antibacterial activity against the two kinds of fungi, and the microorganisms decreased Was confirmed (FIGS. 1 to 4 ).

In a specific embodiment of the present invention, when treating the preservative composition of the present invention, it is remarkably excellent against Bacilus and Burkholderia, although it contains less sodium benzoate than the conventional wet tissue chemical solution. It was confirmed that it exhibits antibacterial activity (Fig. 5).

The preservative composition of the present invention may be one that induces initial microbial death.

In a specific embodiment of the present invention, sodium benzoate of the present invention; And at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; when treated with a preservative composition comprising; it was confirmed that it exhibits an initial microbial death induction effect, in particular sodium benzoate, caprylyl glycol In the case of treatment with lycol and dipropylene glycol, it was confirmed that an excellent initial microbial death induction effect was exhibited (FIGS. 3 and 4 ).

In a specific embodiment of the present invention, when treating the preservative composition of the present invention, excellent antimicrobial activity against Bacilus and Burkholderia, despite containing less sodium benzoate than conventional wet wipes. In particular, when treating the preservative composition of the present invention, it was confirmed that it exhibits remarkably excellent antibacterial activity on Day 1 (FIG. 5).

The preservative composition of the present invention may exhibit stable microbial control.

In a specific embodiment of the present invention, sodium benzoate of the present invention; And at least one selected from the group consisting of caprylyl glycol and dipropylene glycol; when treated with a preservative composition comprising; it was confirmed that it exhibits a stable microbial control effect, in particular sodium benzoate, caprylyl glycol In the case of treatment of Cole and dipropylene glycol, it was confirmed that excellent and stable microbial control effect was exhibited over time (FIGS. 3 and 4 ).

In a specific embodiment of the present invention, when treating the preservative composition of the present invention, excellent antimicrobial activity against Bacilus and Burkholderia, despite containing less sodium benzoate than conventional wet wipes. And it was confirmed that it exhibited an excellent stable microbial control effect over time (FIG. 5).

At least one selected from the group consisting of caprylyl glycol and dipropylene glycol of the present invention may improve the antiseptic power of sodium benzoate.

In a specific embodiment of the present invention, when at least one selected from the group consisting of caprylyl glycol and dipropylene glycol is added to sodium benzoate, antibacterial activity and sodium benzoate preservative improvement effect are shown. It was confirmed that, in particular, when caprylyl glycol and dipropylene glycol were added to sodium benzoate, it was confirmed that excellent antibacterial activity and an effect of improving sodium benzoate preservative ability were exhibited (FIGS. 1 to 4 ).

The preservative composition of the present invention may include 10% to 45% by weight of sodium benzoate based on a total of 100% by weight, and specifically 10% to 45% by weight, 15% to 45% by weight, 20% by weight To 45%, 15% to 40%, 15% to 35%, 20% to 40%, 20% to 35%, 20% to 30%, or 20% to 25% Weight%, more specifically 20% by weight may be included, but is not limited thereto.

The preservative composition of the present invention may contain 0.1% to 10% by weight of caprylyl glycol based on a total of 100% by weight, specifically 0.1% to 10% by weight, 0.2% to 5% by weight, 0.5 Weight% to 3% by weight, 0.5% to 2% by weight, or 0.5% to 1% by weight, more specifically 1% by weight or 0.5% by weight may be included, but is not limited thereto.

The preservative composition of the present invention may contain 0.1% to 10% by weight of dipropylene glycol based on a total of 100% by weight, specifically 0.1% to 10% by weight, 0.2% to 5% by weight, 0.5 Weight% to 3% by weight, 0.5% to 2% by weight, or 0.5% to 1% by weight, more specifically 1% by weight or 0.5% by weight may be included, but is not limited thereto.

Another aspect of the present invention provides a wet tissue liquid containing the preservative composition.

At this time, the description of "sodium benzoate", "caprylyl glycol", "dipropylene glycol", "preservative" and "wet tissue" is as described above.

The term "wet tissue chemical solution" of the present invention refers to a solution impregnated into a wet tissue fabric when manufacturing a wet tissue, and generally includes purified water, a preservative, and other raw materials.

The chemical liquid for wet tissues of the present invention contains substances commonly added in the art, for example, moisturizers, solvents, surfactants, pH adjusters, fragrances, skin softeners, etc., within the range not impairing the effect of the preservative composition. Can, specifically 1,2-hexanediol (1, 2-hexandiol), glycerin (glycerin), glyceryl caprylate (glyceryl caprylate), butylene glycol (butylene glycol), sodium anisate (sodium anisate) , Citric acid, ethylhexylglycerin, polysorbate 20, propylene glycol, and the like may be included, but are not limited thereto.

The chemical solution for wet tissues of the present invention may contain 0.10 wt% to 0.45 wt% sodium benzoate based on a total of 100 wt%, specifically 0.10 wt% to 0.45 wt%, 0.15 wt% to 0.45 wt%, 0.20 wt% % To 0.45 wt%, 0.15 wt% to 0.40 wt%, 0.15 wt% to 0.35 wt%, 0.20 wt% to 0.40 wt%, 0.20 wt% to 0.35 wt%, 0.20 wt% to 0.30 wt% or 0.20 wt% to 0.25% by weight, more specifically 0.20% by weight may be included, but is not limited thereto.

The chemical solution for wet tissues of the present invention may contain from 0.001% to 0.1% by weight of caprylyl glycol based on a total of 100% by weight, specifically from 0.001% to 0.1% by weight, from 0.002% to 0.05% by weight, 0.005 wt% to 0.03 wt%, 0.005 wt% to 0.02 wt%, or 0.005 wt% to 0.01 wt%, more specifically 0.01 wt% or 0.005 wt%, but is not limited thereto.

The chemical solution for wet tissues of the present invention may contain 0.001 wt% to 0.1 wt% of dipropylene glycol based on a total of 100 wt%, specifically 0.001 wt% to 0.1 wt%, 0.002 wt% to 0.05 wt%, 0.005 wt% to 0.03 wt%, 0.005 wt% to 0.02 wt%, or 0.005 wt% to 0.01 wt%, more specifically 0.01 wt% or 0.005 wt%, but is not limited thereto.

The preservative composition of the present invention may be diluted and included in a liquid tissue for wet tissues. Specifically, the preservative composition is 10 times to 1000 times, 20 times to 500 times, 30 times to 300 times, 50 times to 200 times, 70 times to 150 times, 80 times to 130 times, 90 times to 120 times, 90 times It may be diluted to 110 times, more specifically 100 times to be included in the wet tissue chemical, but is not limited thereto.

Another aspect of the present invention provides a wet tissue containing the liquid tissue for wet tissue.

At this time, the description of the "sodium benzoate", "caprylyl glycol", "dipropylene glycol", "preservative", "wet tissue" and "chemical liquid for wet tissue" are as described above.

Another aspect of the present invention provides a method for producing a wet tissue comprising the step of impregnating the nonwoven fabric with the liquid for wet tissue.

At this time, the description of the "sodium benzoate", "caprylyl glycol", "dipropylene glycol", "preservative", "wet tissue" and "chemical liquid for wet tissue" are as described above.

The term "nonwoven fabric" of the present invention refers to the formation of a web in the shape of a sheet by tangling various fibers such as natural, chemical, glass, metal, air, etc. It means a flat structure made by combining. There are various non-woven fabrics for making wet tissues, but fabrics that are commonly used are composed by varying the blending ratio from 100% rayon to 100% PET. The nonwoven fabric used in the present invention is not limited in any configuration, structure, etc., and specifically, Rayon4:PET6 nonwoven fabric may be used, but is not limited thereto.

The method of impregnating the wet tissue liquid into the nonwoven fabric is not particularly limited, and specifically, a method of spraying or immersing the wet tissue liquid onto the nonwoven fabric and then dehydrating may be used, but is not limited thereto.

Another aspect of the present invention provides a method for improving the antiseptic power of sodium benzoate, comprising the step of adding at least one selected from the group consisting of caprylyl glycol and dipropylene glycol to sodium benzoate. .

At this time, the description of the "sodium benzoate", "caprylyl glycol", "dipropylene glycol", "preservative", "wet tissue" and "chemical liquid for wet tissue" are as described above.

The method for improving the antiseptic power of sodium benzoate of the present invention may include adding caprylyl glycol and dipropylene glycol to sodium benzoate.

The preservative composition of the present invention induces initial microbial death and exhibits a stable microbial control effect, and thus can be usefully used in the development of antibacterial wipes.

1 shows the antibacterial activity of caprylyl glycol and the effect of improving sodium benzoate preservative ability.
2 shows the antibacterial activity of dipropylene glycol and the effect of improving sodium benzoate preservative ability.
Figure 3 shows the antibacterial activity of caprylyl glycol and dipropylene glycol and the effect of improving sodium benzoate preservative ability.
4 is a caprylyl glycol; Dipropylene glycol; Caprylyl glycol and dipropylene glycol; shows by comparing the antimicrobial activity and antiseptic enhancing effect.
Figure 5 shows the antimicrobial activity against Bacillus or bulcholderia compared with the existing preservative composition.

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are for illustrative purposes only, and the scope of the present invention is not limited thereto.

Example 1. Analysis of the effect of caprylyl glycol on the improvement of antibacterial activity and antiseptic power

To analyze the effect of caprylyl glycol on the antibacterial activity and improvement of sodium benzoate preservative ability, a chemical solution containing only sodium benzoate was prepared as a control group, and sodium benzoate and caprylyl glycol ( 0.001%, 0.01%, 0.1%, 1% and 5%) was added to prepare a chemical solution. At this time, the sodium benzoate was added in an amount of 0.1% to 0.45%. The pH of the chemical solution was adjusted to slightly acidic using citric acid. Thereafter, 5 g of nonwoven fabric (Rayon4:PET6) was prepared by putting it in a sterile zipper bag, and 13.5 g of the prepared chemical solution was evenly impregnated into the nonwoven fabric. After sufficiently kneading, a wet tissue sample was prepared by standing for 2 to 3 hours at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%.

E. coli, Bacillus, Burkholderia, Pseudomonas aeruginosa and Staphylococcus aureus five kinds of bacteria and Candida and Aspergillus niger ) Consisting of two types of fungi, each of a total of 7 types of microorganisms were adjusted to 10 ⁷ to 10 ⁸ CFU/mL using sterile saline, and mixed in the same amount to prepare. Bacteria and fungi were each inoculated with 1% by weight (185 μl) to the prepared wet tissue sample, and then sufficiently massaged.

The wet tissue samples inoculated with microorganisms were stored at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%, and then inoculated into TSA (Trypticase soy agar) medium over time. After incubating for 2 days in a 32.5 ± 3°C incubator, it was confirmed whether or not the microorganisms decreased over time.

As a result, as shown in FIG. 1, when 0.1% or more of caprylyl glycol was added, it was confirmed that the microorganisms were significantly reduced compared to the control group. In addition, it was confirmed that the initial microbial death was induced and stable microbial control was exhibited over time.

From the above results, it was found that when caprylyl glycol was added to sodium benzoate, excellent antibacterial activity and sodium benzoate preservative improvement effect appeared.

Example 2. Analysis of the effect of dipropylene glycol on antimicrobial activity and antiseptic performance improvement

To analyze the effect of dipropylene glycol on the antimicrobial activity and improvement of sodium benzoate preservative ability, a chemical solution containing only sodium benzoate was prepared as a control group, and sodium benzoate and dipropylene glycol ( 0.001%, 0.01%, 0.1%, 1% and 5%) was added to prepare a chemical solution. At this time, the sodium benzoate was added in an amount of 0.1% to 0.45%. The pH of the chemical solution was adjusted to weakly acidic using citric acid. Thereafter, 5 g of nonwoven fabric (Rayon4:PET6) was prepared by putting it in a sterile zipper bag, and 13.5 g of the prepared chemical solution was evenly impregnated into the nonwoven fabric. After sufficiently kneading, a wet tissue sample was prepared by standing for 2 to 3 hours at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%.

E. coli, Bacillus, Burkholderia, Pseudomonas aeruginosa and Staphylococcus aureus five kinds of bacteria and Candida and Aspergillus niger ) Consisting of two types of fungi, each of a total of 7 types of microorganisms were adjusted to 10 ⁷ to 10 ⁸ CFU/mL using sterile saline, and mixed in the same amount to prepare. Bacteria and fungi were each inoculated with 1% by weight (185 μl) to the prepared wet tissue sample, and then sufficiently massaged.

The wet tissue samples inoculated with microorganisms were stored at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%, and then inoculated into TSA (Trypticase soy agar) medium over time. After incubating for 2 days in a 32.5 ± 3°C incubator, it was confirmed whether or not the microorganisms decreased over time.

As a result, as shown in FIG. 2, when 0.1% or more of dipropylene glycol was added, it was confirmed that microorganisms were reduced compared to the control group. In addition, it was confirmed that it induces microbial death and exhibits stable microbial control over time.

From the above results, it was found that when dipropylene glycol was added to sodium benzoate, the antibacterial activity and the effect of improving the antiseptic ability of sodium benzoate appeared.

Example 3. Analysis of the effects of caprylyl glycol and dipropylene glycol on antibacterial activity and antiseptic performance improvement

In order to analyze the effect of caprylyl glycol and dipropylene glycol on the antibacterial activity and improvement of sodium benzoate preservative ability, as a control group, a chemical solution to which sodium benzoate and caprylyl glycol were added was prepared, and the experimental group As an example, a chemical solution to which sodium benzoate, caprylyl glycol and dipropylene glycol (0.1% and 0.2%) were added was prepared. At this time, the sodium benzoate was added in an amount of 0.1% to 0.45%, and the caprylyl glycol was added in an amount of 0.001% to 0.1%. The pH of the chemical solution was adjusted to weakly acidic using citric acid. Thereafter, the fabric for wet tissue was prepared by putting 5 g each in a sterile zipper bag, and 13.5 g of the prepared chemical solution was evenly impregnated into the fabric. After sufficiently kneading, a wet tissue sample was prepared by standing for 2 to 3 hours at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%.

E. coli, Bacillus, Burkholderia, Pseudomonas aeruginosa and Staphylococcus aureus five kinds of bacteria and Candida and Aspergillus niger ) Consisting of two types of fungi, each of a total of 7 types of microorganisms were adjusted to 10 ⁷ to 10 ⁸ CFU/mL using sterile saline, and mixed in the same amount to prepare. Bacteria and fungi were each inoculated with 1% by weight (185 μl) to the prepared wet tissue sample, and then sufficiently massaged.

The wet tissue samples inoculated with microorganisms were stored at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%, and then inoculated into TSA (Trypticase soy agar) medium over time. After incubating for 2 days in a 32.5 ± 3°C incubator, it was confirmed whether or not the microorganisms decreased over time.

As a result, as shown in FIG. 3, when 0.02% of dipropylene glycol was added to sodium benzoate (0.2%) and caprylyl glycol (0.2%), the microorganisms were significantly reduced compared to the control group. Was confirmed. In addition, it was confirmed that it exhibits excellent effects in inducing initial microbial death and stable microbial control.

From the above results, it was found that when caprylyl glycol and dipropylene glycol were added to sodium benzoate, excellent antibacterial activity and sodium benzoate preservative improvement effect were exhibited even with a small amount of addition.

Example 4. Caprylyl glycol for improving antibacterial activity and antiseptic power; Dipropylene glycol; Comparative analysis of the effects of caprylyl glycol and dipropylene glycol;

Caprylyl glycol for antibacterial activity and improvement of sodium benzoate preservative ability; Dipropylene glycol; In order to compare and analyze the effects of caprylyl glycol and dipropylene glycol; a chemical solution was prepared with the composition shown in Table 1, and the pH of the chemical solution was adjusted to a weak acidity using citric acid.

division Raw material content by option (%) Op.1 Op.2 Op.3 Op.4 Op.5 Sodium benzoate (SB) 0.2 0.2 0.2 0.2 0.2 Dipropylene glycol (DPG) 0 0.01 0 0.01 0.005 Caprylyl Glycol (CG) 0 0 0.01 0.01 0.005

Thereafter, 5 g of non-woven fabric (Rayon4:PET6) was put in a sterilized zipper bag to prepare 8 pieces for each option, and 13.5 g of the prepared chemical solution was evenly impregnated in the non-woven fabric. After sufficiently kneading, a wet tissue sample was prepared by standing for 2 to 3 hours at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%.

E. coli, Bacillus, Burkholderia, Pseudomonas aeruginosa and Staphylococcus aureus five kinds of bacteria and Candida and Aspergillus niger ) Consisting of two types of fungi, each of a total of 7 types of microorganisms were adjusted to McF 0.6 (10 ⁷ to 10 ⁸ CFU/mL) using sterile saline, and then divided into two types of bacteria and fungi, and mixed in the same amount. Each of the bacterial/fungal solutions was inoculated with about 1% (185 μl) to the prepared wet tissue sample, and then sufficiently massaged.

The wet tissue sample inoculated with the microorganism was stored at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%, and after 3 days/10 days elapsed, a chemical solution was taken and diluted to perform the experiment. Bacterial medium was cultured in a 32.5 ± 3°C incubator for 2 days, and fungal medium in a 22.5 ± 3°C incubator for 5 days, and then microbial reduction was confirmed.

As a result, as shown in Table 2 and Figure 4, it was confirmed that all of Op.2 to Op.5 reduced microorganisms compared to Op.1 treated with sodium benzoate alone. In addition, it was confirmed that it induces initial microbial death and shows stable microbial control.

In particular, compared to Op.2 in which sodium benzoate was treated only with caprylyl glycol and Op.3 in which sodium benzoate was treated with only dipropylene glycol, caprylyl glycol and dipropylene glycol in sodium benzoate It was confirmed that the Lycol-treated Op.4 and op.5 showed remarkably excellent initial microbial death induction and stable microbial control effect.

From the above results, caprylyl glycol (Op.3); Dipropylene glycol (Op.2); Caprylyl glycol and dipropylene glycol (Op.4 and Op.5); It was found that both have antibacterial activity and sodium benzoate preservative improvement effect.

division Test result (CFU/ml) Schedule Op.1 Op.2 Op.3 Op.4 Op.5 calendar date Germ Fungi Germ Fungi Germ Fungi Germ Fungi Germ Fungi inoculation 18.09.11 TNTC TNTC TNTC TNTC TNTC TNTC TNTC TNTC TNTC TNTC Day3 18.09.14 TNTC TNTC TNTC TNTC TNTC 610 ND 70 ND 20 Day10 18.09.21 TNTC TNTC 3740 550 1590 150 ND ND 20 10

Example 5. Analysis of antibacterial activity against Bacillus or Vulcholderia

In order to confirm the excellent antibacterial activity against Bacillus or bulcholderia of the preservative composition of the present invention, an existing drug solution-A and an existing drug solution-B were prepared as a control group, and as an experimental group, for a wet tissue containing the preservative composition of the present invention A chemical solution was prepared, and the sodium benzoate content of each chemical solution is as shown in Table 3. In particular, in the case of the existing drug solution-A and the existing drug solution-B, it is a preservative composition that has improved antibacterial activity and antiseptic power against Bacillus and bulcholderia.

division Raw material ingredients by option Sample Existing chemical solution-A Existing liquid-B Furtherance Sodium benzoate, caprylyl glycol, ethylhexyl glycerin, dipropylene glycol, disodium EDTA, glycerin, polysorbate 20, citric acid Sodium benzoate, propylene glycol, sodium anisate, caprylyl glycol, butylene glycol, glycerin, citric acid Sodium benzoate, 1,2-hexanediol, caprylyl glycol, ethylhexyl glycerin, polysorbate 20, glycerin, citric acid Sodium benzoate content (ppm) 3,000 4,500 4,000

A nonwoven fabric (Rayon4:PET6) was prepared by putting 5 g each in a sterile zipper bag, and 13.5 g of the chemical solution was evenly impregnated into the nonwoven fabric. After sufficiently kneading, a wet tissue sample was prepared by standing for 2 to 3 hours at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%.

Each of the two bacteria Bacillus and Burkholderia was adjusted to McF 0.6 (10 ⁷ to 10 ⁸ CFU/mL) using sterile saline, and then about 1% of each bacterial solution was inoculated into the prepared wet tissue sample. After doing (200 μl), it was thoroughly massaged.

The wet tissue sample inoculated with the microorganism was stored at a temperature of 25 ± 3° C. and a humidity of 50 ± 5%, and after 1 day/7 days elapsed, a chemical solution was taken and diluted to perform the experiment. Bacterial medium was cultured in a 32.5 ± 3°C incubator for 2 days, and then it was confirmed whether or not the microorganism was reduced.

As a result, as shown in Table 4 and FIG. 5, it was confirmed that microorganisms were reduced when the wet tissue drug containing the preservative composition of the present invention, the existing drug solution-A and the existing drug solution-B were treated. Specifically, in the case of the wet tissue liquid containing the preservative composition of the present invention, it was confirmed that the microorganisms were significantly reduced on Day 1 and the antibacterial activity persisted even with the passage of time compared to the existing drug solution-A and the existing drug solution-B. It was found that it induces microbial death and shows stable microbial control.

In particular, as shown in Table 3, compared with the existing chemical solution-A (4500 ppm) and the existing drug solution-B (4000 ppm), the chemical solution for wet tissues of the present invention contains a small amount (3500 ppm) of sodium benzoate, Although the existing drug solution-A and the existing drug solution-B are preservative compositions that have already improved antiseptic properties against Bacillus and bulcholderia, the antibacterial activity and antibacterial activity due to the addition of dipropylene glycol and/or caprylyl glycol It was confirmed that it exhibits excellent antibacterial activity against Bacillus and bulcholderia due to the buoyancy enhancing effect.

From the above results, it was found that the preservative composition of the present invention exhibited remarkably excellent antibacterial activity against Bacillus and bulcholderia compared to the existing preservative composition, thereby improving the inherent weakness of wet tissues.

division Test result (CFU/ml) Schedule Sample Existing chemical solution-A Existing liquid-B calendar date Vulcolderia Bacillus Vulcolderia Bacillus Vulcolderia Bacillus inoculation 18.12.27 TNTC TNTC TNTC TNTC TNTC TNTC Day1 18.12.28 220 160 2180 1960 TNTC 1630 Day7 19.01.04 ND 10 ND 20 50 20

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed that all changes or modifications derived from the meaning and scope of the claims to be described later rather than the above detailed description, and equivalent concepts thereof, are included in the scope of the present invention.

Claims (15)

As a liquid tissue for wet tissue containing a preservative composition containing sodium benzoate, caprylyl glycol and dipropylene glycol as active ingredients, the caprylyl glycol or dipropylene glycol is the preservative power of sodium benzoate In addition, the preservative composition has antimicrobial activity against Bacillus or Burkholderia, and the liquid tissue for wet wipes contains 0.001% by weight to 0.1% by weight of caprylyl glycol and It will contain 0.001% by weight to 0.1% by weight of dipropylene glycol, a chemical for wet tissues.
delete delete delete delete The method of claim 1, wherein the preservative composition is to induce initial microbial death, the wet tissue chemical.
The method of claim 1, wherein the preservative composition exhibits a stable microbial control, a wet tissue chemical.
delete delete The method of claim 1, wherein the wet tissue chemical solution contains 0.10% by weight to 0.45% by weight of sodium benzoate based on a total of 100% by weight.
delete delete A wet tissue containing the liquid tissue for wet tissue of claim 1.
A method for producing a wet tissue comprising the step of impregnating the wet tissue liquid of claim 1.
As a method for improving the preservative ability of sodium benzoate, comprising the step of adding caprylyl glycol and dipropylene glycol to sodium benzoate, wherein the caprylyl glycol or dipropylene glycol is To improve the antiseptic power, the composition containing sodium benzoate, caprylyl glycol and dipropylene glycol has antibacterial activity against Bacillus or bulcholderia, and the caprylyl glycol and dipropylene glycol Each of Lycol is added in an amount of 0.001% by weight to 0.1% by weight based on a total of 100% by weight of a wet wipe containing the composition.

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