KR20200135781A - 세포 배양에서 생산된 항체의 총 비푸코실화 당형태 - Google Patents

세포 배양에서 생산된 항체의 총 비푸코실화 당형태 Download PDF

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KR20200135781A
KR20200135781A KR1020207026608A KR20207026608A KR20200135781A KR 20200135781 A KR20200135781 A KR 20200135781A KR 1020207026608 A KR1020207026608 A KR 1020207026608A KR 20207026608 A KR20207026608 A KR 20207026608A KR 20200135781 A KR20200135781 A KR 20200135781A
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South Korea
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culture medium
cell culture
level
fucose
concentration
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Korean (ko)
Inventor
프린스 베베
매튜 제럼스
아이린 리우
커트 쿠나스
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암젠 인크
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Priority to KR1020257043688A priority Critical patent/KR20260011202A/ko
Publication of KR20200135781A publication Critical patent/KR20200135781A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
KR1020207026608A 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태 Ceased KR20200135781A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257043688A KR20260011202A (ko) 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862648308P 2018-03-26 2018-03-26
US62/648,308 2018-03-26
PCT/US2019/024154 WO2019191150A1 (en) 2018-03-26 2019-03-26 Total afucosylated glycoforms of antibodies produced in cell culture

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020257043688A Division KR20260011202A (ko) 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태

Publications (1)

Publication Number Publication Date
KR20200135781A true KR20200135781A (ko) 2020-12-03

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Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020207026608A Ceased KR20200135781A (ko) 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태
KR1020257043688A Pending KR20260011202A (ko) 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태

Family Applications After (1)

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KR1020257043688A Pending KR20260011202A (ko) 2018-03-26 2019-03-26 세포 배양에서 생산된 항체의 총 비푸코실화 당형태

Country Status (15)

Country Link
US (2) US12325737B2 (https=)
EP (1) EP3775251A1 (https=)
JP (2) JP2021519068A (https=)
KR (2) KR20200135781A (https=)
CN (2) CN111954719B (https=)
AU (2) AU2019243848B2 (https=)
BR (1) BR112020019559A2 (https=)
CA (1) CA3093853A1 (https=)
CL (1) CL2020002450A1 (https=)
EA (1) EA202092286A1 (https=)
IL (2) IL276910B2 (https=)
MA (1) MA52186A (https=)
MX (2) MX2020009907A (https=)
SG (1) SG11202009216YA (https=)
WO (1) WO2019191150A1 (https=)

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AU2024256160A1 (en) * 2023-04-20 2025-10-02 Amgen Inc. Methods of determining relative unpaired glycan content

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