KR20200066987A - Process for producing ginseng and pulp fermented with ginseng root using Rizofus seeds and fermented composition prepared by the method - Google Patents

Abstract

The present invention relates to a process for preparing a fermented product of sprout ginseng and beans by using a Rizofus starter, and a fermented composition prepared by the method. More specifically, the present invention relates to a process for preparing a fermented product of sprout ginseng and beans by using a Rizofus starter, and to a fermented composition prepared by the method, wherein the fermented composition may be used as a source material for a main ingredient of regular health food and health functional food, or of regular skincare cosmetics and functional cosmetics; may be provided as a liquid type, a gel type, or a solid type (powder, blocks, or paper) depending on the purpose of use; and is modified so as to contribute to alleviating atopic dermatitis or acne-induced skin inflammation in particular, and to suppressing aging.

Description

Process for producing ginseng and soybean fermentation products using lyzofus spp. and fermented composition prepared by the method{Process for producing ginseng and pulp fermented with ginseng root using Rizofus seeds and fermented composition prepared by the method}

The present invention relates to a method for producing fermented soybean sprouts and soybeans using Lyzopus spp. and, more specifically, the main components of general health foods and health functional foods or general basic cosmetics and functional cosmetics. It can be used as a raw material, and can be provided as a liquid type, gel type, or solid type (powder, block, paper) depending on the use.In particular, it is improved to contribute to improving atopic or acne dermatitis and to suppress aging. It relates to a method for producing fermented ginseng and soybean sprouts using the old Lyzopus spp. and a fermentation composition prepared by the method.

Normally, the amount of ATP in the intracellular mitochondria when a cell is damaged or under-functioned due to a change in diet, medication, surgery, food, environmental conditions, chemical physical factors, pressure, temperature, pH, etc. This decreases.

At this time, the core temperature is lowered, and in the hypothalamus, which is a body temperature control center, the pores are closed to reduce and discharge sweat by preserving and maintaining body temperature.

By the way, if you live with daily work, excessive work, stress, meal, exercise, etc. with the pores closed, the water supply to the skin decreases, and the activity of the sebaceous glands stimulates and the excessive amount of sebum is secreted. And this is directly related to acne production.

Of course, when it comes to acne, hormonal abnormalities, irregular eating habits, and excessive stress may also occur in combination, but the most fundamental and basic cause of development should be considered to be skin drying.

On the other hand, atopic dermatitis is a chronic inflammatory skin disease that occurs during infancy or childhood and repeats improvement and exacerbation, accompanied by rash and severe itching caused by external irritants.

With the development of the industry, accelerated urbanization and industrialization have caused environmental pollution, and such environmental pollution has rapidly increased the occurrence of atopic dermatitis, leading to serious social problems.

The pathogenesis of atopic dermatitis is not yet clear, but pathological factors such as functional deficiency due to hypersensitivity reactions due to an increase in IgE antibody or unstable differentiation of T lymphocytes, which are caused by a decrease in cell-mediated immune function, As it turned out, it is now thought to be a disease involving immunological abnormalities.

In addition, exogenous atopic dermatitis, which accounts for the majority of atopic dermatitis, is caused by an immune mechanism associated with IgE. There are many reports that a delayed immune response by T cell abnormalities is involved rather than an immediate immune response against a specific allergen.

Recently, Th2-related cytokines such as Il-4, which induce IgE production from B cells, have been reported to be the cause of atopy (JS Kang et al., Inhibition of atopic dermatitis by topical application of silymarin in NC/Nga mice.Intl Immunopharm (2008) 8. 1475-1480).

For the improvement, treatment and management of atopic dermatitis, it is common to prescribe a moisturizing agent that maintains moisture on the skin's surface and a steroid hormone to improve the inflammatory response, that is, a topical adrenal cortical hormone preparation.

However, in the case of topical adrenal cortical hormone, there is a problem that causes various skin side effects such as skin atrophy, vasodilation, pigment loss, and swelling filigree, which makes it difficult to use for a long time.

Therefore, development of atopy treatment or supplementary substances using natural new drug candidates with anti-atopy efficacy and very low side effects is underway in the pharmaceutical and cosmetic fields, but various clinical trials and development periods are long, and treatments to do so have been initiated. It is not.

Accordingly, a number of natural raw materials for cosmetic compositions for improving or alleviating atopic dermatitis having various treatment auxiliary functions have been disclosed.

In line with this, there is a need to develop more environmentally friendly and harmless methods such as improvers and anti-aging agents.

Republic of Korea Registered Patent No. 10-1329914 (2013.11.08.), red pepper paste using Lyzopus mycelium and method for manufacturing the same Republic of Korea Patent Publication No. 10-2014-0003197 (2014.01.09.), a composition for external application for skin containing ginsenoside F2 Republic of Korea Patent Publication No. 10-2014-0013795 (2014.02.05.), wrinkle improvement, skin whitening or acne improvement cosmetic composition containing ginsenoside F2 as an active ingredient

The present invention has been created to solve the problems in the prior art as described above, and can be used as a main ingredient raw material of general health foods and health functional foods or general basic cosmetics and functional cosmetics. , It can be provided in gel type or solid type (powder, block, paper), and in particular, it improves atopic or acne dermatitis, and improves lysophos spp. to improve ginseng and soybean fermentation. The main objective is to provide a water production method and a fermentation composition prepared by the method.

The present invention as a means for achieving the above technical problem, the first step of drying the sprout ginseng (200g basis) to a moisture content of 15% or less; A second step in which two or more selected from soybeans, red beans, green beans, mung beans, and kidney beans are mixed and washed in the same weight ratio; A third step of sprouting and washing the beans washed in the second step in a mining condition with a water spray method for 6-8 days; A fourth step of mixing the sprout ginseng dried product obtained in the first step and the sprouted sprouts obtained in the third step into a pressure vessel at a weight ratio of 1:1; A fifth step of sterilizing the blend at a temperature of 90-120° C. for 30 seconds to 1 minute; A sixth step of cooling the boiled blend to 20-30° C. and inoculating 4-6 parts by weight of Lyzopus bacteria based on 100 parts by weight of the blend for fermentation for 22-26 hours; When fermentation is completed, a seventh step of refrigerating and aging the fermented product in a refrigerator compartment maintained at 0-10°C for 2-3 days; Provided is a method for producing a fermented sprout of ginseng and soybeans using Lyzopus spp., comprising: a step 8 of drying the crushed fermented product to a moisture content of 15% or less and then crushing the fermented product to produce a fermented pulverized product.

At this time, in the eighth step, it is also characterized in that 5.5 parts by weight of isoflavone, 5.5 parts by weight of lecithin, and 4 parts by weight of pentylene glycol based on 100 parts by weight of the fermented product before refrigeration and aging.

In addition, the present invention is prepared by the method for producing sprout ginseng and soybean using fermented ginseng sprouts, characterized in that the fermentation pulverized product prepared by the above-described manufacturing method is processed as a liquid or gel type by adding it as a raw material for cosmetic production. Fermented composition is also provided.

According to the present invention, it can be used as a main ingredient raw material of general health foods and health functional foods or general basic cosmetics and functional cosmetics, and can be provided in liquid type, gel type or solid type (powder, block, paper) depending on the use. In particular, it contributes to improving atopic and acne dermatitis, and can have an effect of inhibiting aging.

Figure 1 is an exemplary flow chart showing a method for producing fermented ginseng sprouts and soybeans using Lyzopus spp. in accordance with the present invention.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

Prior to the description of the present invention, the following specific structures or functional descriptions are merely exemplified for the purpose of illustrating the embodiments according to the concept of the present invention, and the embodiments according to the concept of the present invention may be implemented in various forms, It should not be construed as being limited to the embodiments described herein.

In addition, embodiments according to the concept of the present invention can be applied to various changes and may have various forms, so specific embodiments will be illustrated in the drawings and described in detail herein. However, this is not intended to limit the embodiments according to the concept of the present invention to a specific disclosure form, and it should be understood that it includes all modifications, equivalents, and substitutes included in the spirit and scope of the present invention.

The method for producing sprout ginseng and soybean fermentation products using the Lyzopus spp. according to the present invention was released by the Ginseng Herb Processing Team of the Rural Development Administration on February 22, 2011, and ginsenoside F2 contained in ginseng subsides inflammation as a result of animal experiments. It has the characteristics of suppressing itching', and based on the report, it focused on R&D to complete the concept of fermentation.

Of course, regarding the efficacy of ginsenoside F2, a number of domestic patents are disclosed in the form of applications, registrations, and the like.

It is known that ginsenoside F2 is rarely detected in ginseng, white ginseng, or red ginseng, but only in fermented ginseng.

In addition, general saponins such as ginsenosides Rb1, Rb2, Rc, Rd, and Re, which are the most contained in the body of ginseng (including wild ginseng and red ginseng), are not absorbed directly into the human body, but only a part of them (about 5% or so) It is not absorbed until after its components are converted into saponin metabolites (aka specific saponins, secondary metabolites) such as F1, F2, Rg3, ompound-K, M4, etc. by being decomposed by special bacteria or enzymes in the intestines such as Hasegawa bacteria. It has been reported to exhibit efficacy, and its chemical structure is shown in the figure below.

On the other hand, "Ryzopus bacteria" is a mycelium of edible mushroom that grows on banana leaves, contains superdismutase that breaks down lipids that are not good for the human body, and suppresses malignant E. coli by activating immunity in the intestine." Infraphone has been reported.

In addition, soybeans in which Ryozops mycelium is active are rich in vitamin B group, and are effective in treating anemia, and have a function of removing free radicals, that is, antioxidant, and are known to be excellent in adult diseases and anti-aging. It is known to have nutritional properties, bio-functionality, and coordination.

In addition, soybean is one of the representative foods of Korean people, and it is known to have a good effect in preventing adult diseases because it contains abundant protein and bioactive substances.

These soybeans contain carbohydrates, lipids, etc. in addition to the main ingredient, and they have various vitamins and inorganic ingredients such as calcium, phosphorus, iron, and potassium, so our Korean people have traditionally made soybeans into food, tofu, miso, red pepper paste, bean sprouts, Soy milk, etc. have been consumed in a variety that is difficult to list individually.

The present invention is such a general and natural materials that are common in the surroundings, but they cannot be obtained by simple processing, and by cultivating and fermenting them with special bacteria while processing, aging while alleviating symptoms of skin diseases such as atopic and acne dermatitis. It has a feature in developing one of the raw materials such as cosmetics that can also contribute to suppression.

To this end, the method for preparing sprout ginseng and bean fermented products using Lyzopus spp. according to the present invention comprises: a first step of drying sprout ginseng (based on 200 g) to a moisture content of 15% or less; A second step in which two or more selected from soybeans, red beans, green beans, mung beans, and kidney beans are mixed and washed in the same weight ratio; A third step of sprouting and washing the beans washed in the second step in a mining condition with a water spray method for 6-8 days; A fourth step of mixing the sprouted ginseng dried product obtained in the first step and the sprouted sprouts obtained in the third step into a pressure vessel at a weight ratio of 1:1; A fifth step of sterilizing the blend at a temperature of 90-120° C. for 30 seconds to 1 minute; A sixth step of cooling the boiled blend to 20-30° C. and inoculating 4-6 parts by weight of Lyzopus bacteria based on 100 parts by weight of the blend for fermentation for 22-26 hours; When fermentation is completed, a seventh step of refrigerating and aging the fermented product in a refrigerator compartment maintained at 0-10°C for 2-3 days; The refrigerated aged fermentation product is dried to a moisture content of 15% or less, and then crushed to produce a fermentation pulverized product.

Here, the sprout ginseng uses those grown by the method of cultivation of sprout ginseng, which was previously developed by the applicant and developed by technology, and the ingredients analyzed by the Korea Food Research Institute in relation to the related technology development and certification at the time, are as follows: As shown, it can be confirmed that ginsenoside F2 was detected.

(Our sprout ginseng ginsenoside component analysis data-Korea Food Research Institute)

Therefore, in the present invention, since the ginsenoside F2, which is not easily obtainable from ordinary ginseng, is obtained, the ginseng cultivated by the new ginseng cultivation method developed by the present applicant must be used, and the cultivation method is based on moss, impregnation or chungtae. It is a method of cultivating new sprout ginseng and then cultivating it through nutrient solution.

At this time, the nutrient solution is a mixture composition of water compared to pegmatite (7/1,000), water-soluble SiO ₃ (3/1,000), sulfur (1/10,000), AHCC (Active Hexose Correlated Compound) (2/1,000), and parentheses The mark inside indicates that the reference amount of water is ml, and the dilution amount of the added component is expressed in g. For example, the meaning of pegmatite (7/1,000) compared to water is diluted by adding 7 g of pegmatite fine powder to 1,000 ml of water. It means one, and similarly, water-soluble SiO ₃ (3/1,000) means diluted by adding ₃ g of water-soluble SiO _{3 to} 1,000 ml of water.

In addition, the reason for maintaining the moisture content of the sprout ginseng at 15% or less (preferably 10-15%) in the first step is to ensure fermentation stability, and if it is too high, it is easy to rot, and if it is too low, it dries out and ferments. Sluggishness may occur.

In addition, the beans used in the second and third stages are fermented with the mushroom mycelium, Lyzopus, and the nutrients of soybeans, Isoflavone-based Genistein, Daidzein and Factor2, and vitamin B complex (B2, B6, B12) 2 As the primary metabolite is activated, it lowers the cholesterol content in the blood and prevents arteriosclerosis, prevents oxidation of unsaturated fatty acids, increases the antioxidant effect, and performs natural antibiotic functions.

In particular, it enhances the usefulness of the human body because it has the effect of rapidly promoting absorption in the body. For this reason, in the present invention, the bean must be used in a state in which germination and rooting are to occur.

Subsequently, in the process of fermentation after inoculation of bacteria, various macromolecular organic substances (nutrients, ginsenosides, etc.) contained in the respective materials are finely decomposed by metabolism (fermentation) of Lyzopus bacteria and changed into new substances of low molecular weight. do.

In the process of changing to these new substances, the pharmacological functional efficacy becomes much larger than before fermentation by increasing the content of existing functional ingredients and increasing the absorption capacity of the human body, and also physiologically active functionalities such as vitamins, amino acids, enzymes stabilized through the fermentation process Many new substances with excellent efficacy are synthesized (generated).

In particular, ginsenosides, a high molecular compound, can only be degraded through the gut microbial pravoteloraris. About 38% of Koreans do not have these gut microbes, making them difficult to absorb. However, as can be seen from the existing fermented red ginseng or fermented black ginseng, the ginseng polymer component of sprout ginseng is also decomposed into low molecules through the fermentation process, greatly improving the absorption rate in the human body, and in the process of changing into a new component through the fermentation process. It is directly absorbed into the human body and has a variety of pharmacological and functional effects, so its effective value is high. It produces a large amount of Compound K, ginsenoside Rg3, and ginsenoside F2.

In addition, the complex mycelium metabolite, which is the result of fermentation, activates the cellular function of each body to strengthen the skin barrier or activates and strengthens the characteristic tissue of each skin layer to improve wrinkles (lifting effect), whitening, shrink pores, prevent aging, and various skin Efficacy such as trouble relief will work.

In particular, the fermentation composition according to the present invention expects an effect of further increasing the anti-cancer and anti-aging effects of anti-cancer and anti-aging effects such as ameliorating and improving the atopic and acne dermatitis of existing sprout ginseng and soybeans and inhibiting free radicals and antioxidant activity. In addition, it is expected that new substances will be synthesized (generated) through the decomposition and change of various organic substances in the fermentation (microbial metabolism) process, thereby increasing the various and superior pharmacological auxiliary functions in the human body or skin tissues than before.

Thus, in the present invention, in the seventh step, 5.5 parts by weight of isoflavone, 5.5 parts by weight of lecithin, 5.5 parts by weight of pentylene glycol, based on 100 parts by weight of fermentation product before refrigeration so as to contribute to antioxidant, inhibition of free radicals, and inhibition of aging. More parts can be added.

At this time, the isoflavones have been reported to be effective in preventing metabolism and wrinkles by activating the metabolism of collagen, and are added to further enhance these properties.

In addition, the lecithin is added to prevent skin aging and skin deposition by helping the skin to shine and shine through moisture control in the cells, and help absorb fat-soluble vitamins.

In addition, the pentylene glycol is a colorless or pale yellow liquid that is well mixed with water and oil-soluble solutions, has good antibacterial activity, has excellent biodegradability, and has the ability to attract moisture, contributing to drying control through moisturizing the skin. Is added to.

In addition, in the present invention, in order to promote fermentation while inhibiting the activity of harmful bacteria in the fermentation process in the sixth step, and further enhance the skin absorption, 8 parts by weight of the red rose extract, DCHF-DA ( Dichlorodihydrofluorescin diacetate) can add 6 parts by weight, 4.5 parts by weight of a salt powder, and 0.5 parts by weight of liquid GABA (γ-aminobutyric acid).

At this time, Hwangnyeon is a perennial herbaceous plant belonging to the family Asteriskaceae, and contains medicinal ingredients such as Berberine, Coptisine, Warenine, and Palmatine. It has a property of suppressing the occurrence and is added to utilize this property.

Particularly, the method of obtaining a red lotus extract from Hwangnyeon is finely chopped with dried roots of Hwangnyeon and immersing 1200 g in 70% methanol for 24 hours at room temperature to extract extracts by repeating five times to obtain an extract, which is filtered with filter paper. Next, concentrated under reduced pressure at 60°C, the concentrated extract was lyophilized for 24 hours, powdered, and dissolved in alcohol to have a concentration of 1-5%.

And, the DCHF-DA (dichlorodihydrofluorescin diacetate) is an extract extracted from beet (Veta vulgaris), which acts to enhance the antioxidant properties of the skin and stabilize the pH.

In addition, the sputum powder is obtained by slicing the throat of the ginger tree, placing it in 1% saline at 90°C for 24 hours, filtering the extracted extract, concentrating it with a rotary vacuum concentrator, and freeze-drying it for 72 hours in a freeze dryer. Use the old one. These salts are reported to have an excellent antioxidant effect, collagen synthesis effect and anti-inflammatory effect, as well as excellent mineral supply effect due to the anti-inflammatory properties.

In addition, the liquid GABA is a kind of fermentation liquid, called gamma aminobutyric acid or gamma aminobutyric acid, and added to promote fermentation. However, since it is also a neuromodulator of mammals, it should be added in trace amounts.

By configuring in this way, the present invention is sufficient to confirm that it has a function of alleviating skin diseases based on the research report contents published by the Rural Development Administration and the results of the analysis of the components extracted from the sprout ginseng of the applicant, without describing a separate embodiment. Reveals.

Claims (3)

A first step of drying the sprout ginseng (based on 200 g) to a moisture content of 15% or less;
A second step in which two or more selected from soybeans, red beans, green beans, mung beans, and kidney beans are mixed in the same weight ratio and washed;
A third step of sprouting and washing the beans washed in the second step in a mining condition with a water spray method for 6-8 days;
A fourth step of mixing the sprouted ginseng dried product obtained in the first step and the sprouted sprouts obtained in the third step into a pressure vessel at a weight ratio of 1:1;
A fifth step of sterilizing the blend at a temperature of 90-120° C. for 30 seconds to 1 minute;
A sixth step of cooling the boiled blend to 20-30° C. and inoculating 4-6 parts by weight of Lyzopus bacteria based on 100 parts by weight of the blend for fermentation for 22-26 hours;
When fermentation is completed, a seventh step of refrigerating and aging the fermented product in a refrigerator compartment maintained at 0-10°C for 2-3 days;
A method for producing a fermented sprout of ginseng and soybeans using Lysopus spp., comprising: a step 8 of drying the crushed fermented product to a moisture content of 15% or less and then pulverizing the resulting fermentation product.
According to claim 1,
In step 8, before refrigeration, 5.5 parts by weight of isoflavone, 5.5 parts by weight of lecithin, and 4 parts by weight of pentylene glycol, based on 100 parts by weight of fermentation, are added. Fermentation method.
A fermentation composition prepared by the method for producing fermented ginseng and soybean sprouts using Lyzopus spp., characterized in that the fermented pulverized product manufactured by the manufacturing method according to claim 2 is added as a raw material for cosmetic production and processed into a liquid or gel type. .

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