KR20190104470A - A composition for preventing or improving skin wrinkle comprising herb extracts fermentation products thereof - Google Patents

Abstract

The present invention relates to a composition for preventing or reducing skin wrinkles, including a medicinal herb fermented extract. A composition comprising a medicinal herb fermented extract which consists of Rehmanniae Radix Preparata, Astragalus Root, green tea, peony root, Cornus officinalis, Pueraria lobata Ohwi, Schisandra Fruit, Angelica gigas Nakai, Cnidium officinale, cinnamon, cummin, and pig placenta, honey, and cosmetically, pharmaceutically, or foodlogically acceptable salt as active ingredients, has excellent collagen biosynthetic performance and collagen lyase inhibiting effect, thereby having an effect of reducing skin wrinkles. Also, the medicinal herb fermented extract which consists of Rehmanniae Radix Preparata, Astragalus Root, green tea, peony root, Cornus officinalis, Pueraria lobata Ohwi, Schisandra Fruit, Angelica gigas Nakai, Cnidium officinale, cinnamon, cummin, and pig placenta, and honey, which are active ingredients of the composition for preventing or reducing skin wrinkles of the present invention, have no cytotoxicity and side effects on skin, thereby being safely used in a cosmetic composition, a pharmaceutical composition and a food composition.

Description

{A COMPOSITION FOR PREVENTING OR IMPROVING SKIN WRINKLE COMPRISING HERB EXTRACTS FERMENTATION PRODUCTS THEREOF}

The present invention relates to a composition for preventing or improving skin wrinkles, including herbal extract extract fermentation, and more specifically, Sukjiwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cheongung, Cinnamon, Tournament and Donn placenta It relates to a cosmetic composition, a pharmaceutical composition and a food composition for preventing or improving skin wrinkles comprising the extract fermentation and honey as an active ingredient.

Skin is an organ in which aging occurs primarily, and many studies have been conducted in the cosmetic field to delay or prevent it. Normally, skin aging begins about 25 years of age, and skin aging proceeds in earnest around 40 years of age. As a typical phenomenon of skin aging, sebum secretion is reduced, skin is dried, cell regeneration is slowed, and aging keratin is accumulated a lot of skin roughness. In addition, the amount of collagen that supports the epidermis is reduced, and elastin (elastic fiber) is denatured to cause wrinkles. In addition, the color of the skin stains, blemishes, blotch and pigmentation symptoms appear, the epidermis becomes thinner and the skin protection function is weakened. Other phenomena include an increase in skin problems due to a decrease in the barrier effect associated with a decrease in skin thickness. Researches related to skin aging can be classified into materials that protect skin from ultraviolet rays and active oxygen, which are the main causes of skin aging, materials to improve skin wrinkles, materials to enhance skin elasticity, materials to enhance skin moisturizing power, and to suppress blemishes. have.

As the skin ages, the changes in the dermis appear remarkably, and dermis atrophy after 70 years is a representative aging phenomenon. Changes in the dermis are caused by changes in substances with large molecular weight in the extracellular matrix due to a decrease in the number of fibroblasts and their ability to synthesize. Specific changes include separation of collagen bundles, reduced point polysaccharide synthesis, decreased collagen and elastic fiber counts and diameters, grinding collagen and elastic fibers, and expansion of blood vessels. Generally, collagen, a collagen degrading enzyme that reduces collagen production and collagen content, has the greatest effect on wrinkle formation among various complex factors such as skin moisture content, collagen content, and immune function to the external environment. Expression and activity of degrading enzymes.

Therefore, research and development of functional cosmetics having a skin wrinkle improvement effect in the cosmetic field is actively progressing, and in particular, research using natural materials in order not to cause toxicity or irritation to the skin continues.

Accordingly, the present inventors have tried to develop a naturally occurring compound having excellent skin wrinkle improvement and skin aging prevention effect and high safety and stability without skin side effects. As a result, a composition comprising Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Tournament, and Placenta extract fermentation can increase collagen synthesis and inhibit collagenase activity. The present invention was completed by confirming.

Therefore, the technical problem to be solved in the present invention is to provide a composition for preventing or improving skin wrinkles excellent in collagen synthesis ability, excellent in inhibiting collagen degrading enzyme activity.

In order to solve the above technical problem, in the present invention, a mixed extract fermented product consisting of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, meet flavor and placenta, and its cosmetically acceptable Provided is a cosmetic composition for preventing or improving skin wrinkles including possible salts.

In the present invention, skin anti-wrinkle including mixed extract fermentation consisting of sorghum, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, scented and placenta and honey and a pharmaceutically acceptable salt thereof Provided are pharmaceutical compositions for improvement.

In the present invention, skin anti-wrinkle including mixed extract fermentation consisting of ripe koji sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, geunyang and placenta and honey and food acceptable salts thereof It provides a food composition for improvement.

According to one preferred embodiment, in the present invention 2 to 10% by weight, sulfur 2 to 10% by weight, green tea 2 to 10% by weight, peony 2 to 10% by weight, cornus oil 1 to 8% by weight, brown root 1 to 8 % By weight, 1 to 8% by weight of Schisandra chinensis, 0.5 to 5% by weight, 0.5 to 5% by weight celestial, 0.5 to 5% by weight of cinnamon, 0.1 to 2% by weight of fennel, 1 to 3% by weight of placenta and 40 to 60 honey It provides a composition for preventing or improving skin wrinkles by weight percent.

In the present invention, the "extract" is an extract extracted by the conventional method of the ripening sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, convention flavor and placenta, Peony, cornus, brown root, Schizandra chinensis, Angelica, Cheongung, Cinnamon, Tournament, and placenta extract, as well as its dry powder or any form formulated using it.

The extract may be extracted using water or an organic solvent, and the extracted liquid may be used in liquid form or concentrated and / or dried. The organic solvent is methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1 , 3-butylene glycol, propylene glycol, or a mixed solvent thereof, and can be extracted at room temperature or warmed under conditions in which the active ingredient of the extract is not destroyed or minimized.

In one specific embodiment, the extracts of Sukji Hwang, Astragalus, Green Tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cheongung, Cinnamon, Tournament, and Placenta extract were added 10 times (w / v) of distilled water for 4 hours. After extraction, the process of collecting the supernatant with a filter paper may be repeatedly collected three times, followed by drying under reduced pressure.

The extraction method is not limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction and the like.

The mixed extracts of Sukji sulfur, Astragalus, green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Chun-Kung, Cinnamon, Tournament and Placenta of the present invention are all extracts obtained at each stage of extraction, fractionation, or purification (separation, fractionation), Fractions, purified products, dilutions thereof, concentrates, or dried products.

In addition, in the present invention, the extraction sites of Sukjihwang, Astragalus, green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cheongung, Cinnamon, Tournament fragrance can be used all parts such as stems, roots, leaves, flowers, especially the root is preferred. It is not limited to any specific site | part.

In the present invention, "extracted fermented product" means the fermented extract in lactic acid bacteria. At this time, the lactic acid bacteria are used as fermentation bacteria, for example, Lactobacillus casei (Lactobacillus casei, Lactobacillus plantarum , L. acidophilus , L. bulgaricus , Bifidobacterium longum , Bifidobacterium B. bifidum or mixed bacteria thereof may be used, and preferably, L. plantarum, B. bifidum or mixed bacteria thereof may be used. In particular, Lactobacillus plantarum (L. plantarum ) is preferred.

In a preferred embodiment of the present invention, the extract fermentation is lactic acid bacteria in the mixed extract of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, geunyang and placenta 4 to 6 based on the weight of the extract It can be prepared by inoculating in an amount of% by weight and incubating for 10 to 20 hours.

The mixed extract fermentation product consisting of the ripening sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, celcius, cinnamon, scented scent and placenta of the present invention and honey or its cosmetic, pharmaceutical or food acceptable salt Skin wrinkle improvement composition comprising as an active ingredient has a purpose of preventing skin aging or skin wrinkle improvement.

The composition of the present invention, while having low cytotoxicity, exhibits excellent anti-wrinkle effect through the mechanism of promoting collagen synthesis and the inhibitory activity of collagen degrading enzyme, which is clearly confirmed by the following examples.

Skin wrinkle improvement, which is the use of the compositions of the present invention, is understood to include conventional skin protection uses (prevention of wrinkles, removal of wrinkles, prevention or treatment, etc.).

In specific embodiments of the present invention, collagen biosynthesis, collagen degrading enzyme activity, and the like, which are generally used for screening of wrinkle improvement substances, are measured. As a result, a composition containing a mixed extract fermentation and honey consisting of Sukjihwang, Astragalus, Green Tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Tournament and Placenta, not only promote collagen production and cell proliferation, but also collagen The effect of inhibiting the enzyme activity can also be observed.

Sukjiwang , an active ingredient of the composition of the present invention, is processed from the roots of Rehmannia glutinosa Liboschitz var.purpurea Makino or R. glutinosa Liboschitz , and is used for the treatment of blood deficiency and consequent malnutrition of the liver and heart. It is known to strengthen the tone of the kidneys. The lack of liver and heart nutrition causes symptoms such as back pain, dizziness, dizziness, tinnitus, hearing loss, periodic fever, and cold sweating. Suk Ji Hwang is known to replenish the worms (jing in Chinese, essence in English) and to fill bones. It is known that it can alleviate hunger cough, wheezing, and asthma caused by malnutrition of kidneys. As described above, some effects on sulfur of Sukji have been known, but no studies on the effect of inhibiting collagenase activity have been made.

Astragalus membranaceus Bunge ( Astragalusmembranaceus Bunge ) is an active ingredient of the composition of the present invention grows in the mountains and reaches a height of 1m and has fine hairs on the whole. A leaf is a rider's right upper lobe consisting of 6-11 pairs of leaflets. Flowers bloom in July and August, 15 to 18 mm long, light yellow, with several flowers alternated with long flowers, forming inflorescences. The root is used as a medicine, and the active ingredient is folic acid and choline. In animal experiments, excitatory and diuretic effects of the central nervous system were also remarkable. When a large amount of powder was administered to rats, it inhibited the development of nephritis, delayed the development of proteinuria and cholesterol, and lowered blood pressure. In addition, it is widely used in the uterine sewage, stomach and drainage, dehonghong, uterine bleeding, and also improves the strength and tension of the whole body muscles.

Green tea, an active ingredient of the composition of the present invention, uses the leaves of Camellia sinensis as a raw material. The youngest leaves from the newly sprouted branches are used to make tea, and the leaves are picked in May, July, and August, and the best tea is picked in May. This tea tree is an evergreen and grows well in relatively warm and heavy areas. To make green tea, the other leaves are immediately heated to destroy oxidase to keep the green intact, while evaporating moisture to make the leaves dull and dry. Recently, tea is manufactured using a steamer, an oil tanker, a base oil, a rebuilder, an oil refiner, and a dryer.

Peony (Paeonialactiflora), the active ingredient of the composition of the present invention, is a perennial herb of the dicotyledon and peony, which is about 1m high and is divided into white peony or red peony depending on the flower color. Peony contains ingredients such as aspartic acid and benzoic acid to help relieve headaches and toothaches. In addition, it removes the blood from the body and smooths the blood circulation to clear blood and improve women's diseases.If you take peony continuously, it suppresses the secretion of gastric acid, relaxes the stomach and relieves stomach cramps and stomach ulcers, neuralgia or It is also effective for stomach ache.

Cornus officinalis , the active ingredient of the composition of the present invention, is a fruit of the cornus tree, which is a deciduous arborescent of the dogwood. Oval nucleus (核果) is initially green and ripens red in August-October. Seeds are long oval, with ridges. Steamed with strong sweetness and strong acidity. Harvested after mid-October in October, meat and seeds are separated and meat is used for alcohol, tea and herbal medicine. The flesh contains glycosides such as cornin, moroniside, loganin, tannin, saponin, and organic acids such as wine, malic acid, tartaric acid, etc. In addition, vitamin A and a large amount of sugar are included. Seeds contain palmitic acid, oleic acid, linoleic acid, and the like. Among the components, cornine is known to have an excitatory action of parasympathetic nerves. From ancient times, the herbal medicine was medicinal. "Consent of the sense of agreement,""Hyangyakseongbang", etc. are said to be effective, such as Gangeum (强 陰), Sinjeong (腎精) and Shingi (腎氣) reinforcement, convergence. It is used as a medicine for headache, tinnitus, seawater disease, fever, excessive menstruation, and also used for folk remedies such as cold sweat and nocturnal enuresis. Tea is also brewed with alcohol, cold, (보) and the effect of (음) is effective. Cornus trees are native to Korea and China, and are planted in sub-central Korea. In Korea, they are shipped as special products every year from Shandong-myeon, Gurye-gun, Jeollanam-do, Baeksa-myeon, Icheon-si, Gyeonggi-do, and Uiseong-gun, Gyeongsangbuk-do.

Puerariae Radix , an active ingredient of the composition of the present invention, dried roots of perennial herb, Pueraria lobata Ohwi , is native to Korea, China, and Japan. . In Chinese medicine, the various parts of 칡 are classified, and the roots of dried 갈 are called dry roots, flowers are browned, fruits are cracked or browned, leaves are browned, and vines are brown, and they have been used differently depending on the symptoms and treatment methods of the disease. . In the oriental medicine, the roots are used to relieve the muscles, so it is used for the purpose of releasing cold body swelling, rash, anti-alcoholic pain, etc. It acts as a branch.

Schisandra chinensis , an active ingredient of the composition of the present invention, has a ball shape of about 1 cm in diameter and is dark red in color. The inside contains 1 to 2 red juice and reddish brown seeds. It has five flavors: sweet, sour, bitter, salty, and spicy, with strong acidity. Kinds include Schisandra chinensis (North Schisandra chinensis), South Schisandra chinensis, Black Schisandra chinensis. Omija grows mostly in the Taebaek Mountain area, South Omija grows in the southern island region, and Black Omija grows in Jeju Island. It is produced in Korea, Japan, Sakhalin Island and China. It contains ingredients such as xanthrin, gomisin, citric acid, malic acid, and citric acid, which strengthens the heart, lowers blood pressure, and boosts immunity. Strong lung function, cough, expectorant action to help treat coughs and thirst. Soak dried fruit in cold water, add honey and sugar to reddish water, drink it as a drink, or make a flower or starch. Boil chestnuts, jujube, and samsam together to make tea or make alcohol.

Angelica Gigas , an active ingredient of the composition of the present invention belongs to the Anaphylaceae, and has the property of healthy and warm body of five gut, the body of the donkey has the effect of controlling the blood and the upper part of the donkey is blood .

Cnidium officinale , an active ingredient of the composition of the present invention, is a perennial herbaceous herbaceous herb, 40-70 cm in height, which is a fragrant plant, which heals wounds, regenerates skin, and acts as a fungus. In addition, it is excellent in the melanocyte stimulating hormone inhibitory effect excellent whitening effect.

Cinnamon as an active ingredient of the composition of the present invention refers to the bark of the bark of the camphor tree ( Cinnamomumcassia Blume : 또는) or the bark of the same plant. The broiler tree is an evergreen and tall tree that grows in southern China and uses bark from summer to autumn. The drug has a peculiar smell, and later it is slightly mucus, astringent, tastes hot and sweet and hot. Cinnamon promotes blood circulation and eliminates coldness in the chest, promotes appetite, promotes digestion, and is used for limb paralysis. As a pharmacological action, the effect of inhibiting ameliorating bacteria, inhibiting white bacteriophage, healthy, and saliva and gastric juice secretion were reported.

Conventional scent of the composition of the present invention is the same as fennel (Feniium vulgare ), also known as octagonal fennel (Illicium verum), and refers to the fruit of magnolia and evergreen. After drying, the fruit is powdered and used as a spice. When added to long cooked dishes such as steamed or boiled duck or pork dishes, it removes the bad smell of the main ingredients and serves to enhance the taste of the dish. And diuretic and appetite boosting and abdominal bloating or nausea to reduce the action. In China, it is used as a herbal medicine for the treatment of back pain, constipation and cystitis. It is also used to relieve colic or acute rheumatism. It is also used to flavor cough medicine.

The placental placenta (pig placenta), an active ingredient of the composition of the present invention, was found to be remarkably similar to the molecular structure of the placenta in mammalian placenta, which is an important component of protein and various nutrients and DNA and RNA. It has been reported as a source of bio-active cytosin that controls differentiation and fetal development.

The placenta is an organ that develops in the womb of a pregnant mammal and nourish and sustain life through the umbilical cord. The placenta is discharged from the uterus at birth and the placenta is called postpartum. The placenta contains essential amino acids, melatonin, nucleic acids such as RNA and DNA, and SOD (Super Oxide Dismutase), an antioxidant enzyme. Hyaluronic acid, antioxidants, cytokines, placental peptides, insulin-like-growth factors, epidermal growth factors (EGFS) and unique senile cell activity Growth factors such as factors (SCAFS, Senescent Cell Adivating Factors) and cytokines are included and are known to be useful for fatigue recovery and immune augmentation. In addition, the placenta has the effect of inhibiting the activity of the enzyme tyrosinase, which promotes the production of melanin granules from tyrosine, inhibits pigment formation and promotes the release of cells containing melanin pigment, thereby preventing spots. It is also effective in improving and eventually whitening as well as preventing pigmentation (Mallick S et el., Pigment Cell Res. Feb 18 (1), 25-33, 2005). In addition, the tissue metabolism promoting action of the placenta increases sebum secretion function to normalize the secretion of sebum as a result of reducing or removing the wrinkles. In addition, the placenta contains fibroblast growth factor (FGF), which promotes fibroblast proliferation and regeneration, and through its activation increases the amount of collagen, elastin, and hyaluronic acid to restore elasticity and elasticity of the skin to deep wrinkles. And it will act to improve skin sagging.

As the placenta extraction method, conventionally, a method of hydrolyzing the placenta using strong acid (usually 3N hydrochloric acid) or strong alkali to obtain the product thereof, a placenta extraction method using an enzyme, and the like have been used. Korean Patent Laid-Open No. 10-2007-0004477 describes a method for preparing placental extract through alkaline hydrolysis, and Korean Patent No. 0642080 describes a method for preparing placental extract through plant enzyme and acid hydrolysis. In Japanese Patent Laid-Open No. 2004-097033, a method for producing placenta extract using protease is disclosed, and in Korean Patent No. 0642681, a method for producing placenta extract using a supercritical fluid extraction process. It describes.

The mixed extract fermentation product consisting of the ripening sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, celcius, cinnamon, scented scent and placenta of the present invention and honey or its cosmetic, pharmaceutical or food acceptable salt Containing the composition shows a skin wrinkle improvement effect, there is little cytotoxicity as a natural substance.

According to one embodiment of the present invention, a mixed extract fermentation consisting of ripening sulfur, astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, Cinnamon, cinnamon, scented and placenta and honey or a cosmetically acceptable salt thereof It provides a cosmetic composition comprising the as an active ingredient.

In a preferred embodiment of the present invention, the medicinal herb extract fermentation and honey consisting of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, Cinnamon, Cinnamon, Tournament and Donnta are based on the total composition from 0.00001 to 15.0% by weight, more preferably 0.0001 to 10% by weight, most preferably 0.0001 to 5% by weight.

The cosmetic composition according to one embodiment of the present invention further contains components conventionally added to the cosmetic composition in addition to the active ingredient, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. Can be added.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a nourishing cream, astringent lotion, a flexible lotion, a lotion, an essence, a nourishing gel or a massage cream.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant gum, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier components. Can be.

Toss, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component when the formulation of the present invention is a powder or a spray, and in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Castle cellulose, aluminum metahydroxy, bentonite, agar or tracant gum can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The pharmaceutical composition according to one embodiment of the present invention is a pharmaceutically acceptable carrier in addition to the mixed extract fermentation consisting of Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Chunung, Cinnamon, Tournament and Placenta It includes. Pharmaceutically acceptable carriers included in the pharmaceutical compositions of the present invention are those commonly used in the preparation of lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like It doesn't happen. In addition to the above components, the pharmaceutical composition of the present invention may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention may be administered orally or parenterally, and is preferably applied by topical application by parenteral administration, more preferably by application.

Suitable dosages of the pharmaceutical compositions of the present invention vary depending on factors such as the formulation method, mode of administration, age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion and response to response of the patient. Can be. The dosage of the pharmaceutical composition of the present invention is in the range of 0.001-100 mg / kg on an adult basis. In addition, in the case of external preparations, it is recommended to continue the application for 1 month to 5 times a day in an amount of 1.0 to 3.0 ml on an adult basis. However, the dosage does not limit the scope of the present invention.

The pharmaceutical compositions of the present invention may be prepared in unit dosage form or in multidose form by formulating with a pharmaceutically acceptable carrier and / or excipient, according to methods which can be easily carried out by those skilled in the art. It may be prepared by incorporation into a container. In this case, the formulation may be in the form of a solution, suspension, syrup or emulsion in an oil or an aqueous medium, or may be in the form of an exir, powder, powder, granule, tablet or capsule, and may further include a dispersing or stabilizing agent.

In addition, the food composition according to an embodiment of the present invention, as an active ingredient, as well as mixed extract fermented products and honey consisting of sujijihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheongung, cinnamon, meet fragrance and placenta As well as ingredients conventionally added in the manufacture of food, such as proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents.

Examples of such carbohydrates are monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

For example, when the food composition of the present invention is prepared with a drink, in addition to the extract fermentation of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, tofu extract, jujube extract, licorice extract, etc. Can be.

On the other hand, the mixed extract fermentation and honey consisting of Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cinnamon, Cinnamon, Tournament, and Placenta, which are the active ingredients of the composition of the present invention, are harmless to humans, toxic and Since there are few side effects, it can be used safely even in long-term use, and in particular, it can be safely applied to cosmetics, pharmaceutical and food compositions as described above.

As such, the herbal extract extract fermentation and honey and its cosmetic, pharmacological or foodstuffs, which are made of Sukjiwang, Astragalus, green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cheongung, Cinnamon, Tournament and Placenta of the present invention The composition containing a possible salt as an active ingredient is excellent in collagen biosynthesis, excellent in inhibiting collagen degrading enzymes and has an effect of improving skin wrinkles. In addition, herbal medicine extract fermentation and honey consisting of Sukjiwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Chun, Cinnamon, Tournament and Placenta, which are the active ingredients of the composition for preventing or improving skin wrinkles of the present invention, It is free of cytotoxicity and skin side effects and can be safely used in cosmetic compositions, pharmaceutical compositions and food compositions.

The following drawings, which are attached to this specification, illustrate preferred embodiments of the present invention, and together with the contents of the present invention serve to further understand the technical spirit of the present invention, the present invention is limited to the matters described in such drawings. It should not be construed as limited.
1 is a graph showing the measurement of collagen biosynthesis of the composition according to the present invention.
Figure 2 is a graph showing the measurement of collagen degrading enzyme inhibitory effect of the composition according to the present invention.
3 to 5 is a clinical picture showing the wrinkle improvement effect of the beverage containing the herbal medicine extract according to the present invention.

Hereinafter, examples and the like will be described in detail to help understand the present invention. However, embodiments according to the present invention can be modified in many different forms, the scope of the invention should not be construed as limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Preparation of a Composition Comprising Extracted Fermentation of Sukjihwang, Astragalus, Green Tea, Peony, Cornus, Brown Root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Tournament and Placenta

Dried ripening sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheongung, cinnamon, geunyang and placenta were added 10 times (w / v) of distilled water and extracted at 90 ° C for 4 hours. Recovered. This process was collected three times, and then dried under reduced pressure and stored at -20 ° C.

It was mixed at the composition ratio as shown in Table 1.

Hungjiwang Astragalus green tea Peony Cornus Rooting Schisandra Donkey Cheongung cinnamon Competition Placenta honey weight% 6 6 6 6 4.8 4.8 4.8 3 3 3 0.8 1.8 50

Example 2 Preparation of a Composition Comprising Extracted Fermentation of Sukjihwang, Astragalus, Green Tea, Peony, Cornus, Brown Root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Tournament and Placenta

Lactobacillus plantarum (L. plantarum ) bacteria in the extracts of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, Chun, Cinnamon, Tournament and Placenta, respectively, obtained in Example 1 Inoculated in an amount of 5% by weight based on the culture for 12 hours to prepare an extract fermentation.

The composition comprising the extract fermentation was prepared by mixing the extract fermentation and honey of Sukji sulfur, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, gyori and placenta prepared above.

Test Example 1 Measurement of Collagen Biosynthesis

By measuring collagen biosynthesis, the wrinkle improvement effect of the composition according to the present invention was observed. Human normal fibroblasts were seeded into 24-well microplates containing DMEM medium (2 × 10 ⁵ cells / well) and incubated at 37 ° C. for 24 hours in a CO ₂ incubator at 5% concentration. . After 24 hours, the medium was removed from each well and the samples prepared in Examples 1 and 2 were processed and then incubated for 24 hours. After 24 hours, cell media were collected to prepare samples.

The amount of collagen synthesized was measured using a collagen measurement kit (Procollagen Type I C-peptide EIA kit MK101, Takara, Japan) according to the kit manual of Takara Co., Ltd. (procollagen) Type I C-peptide (Type I C-peptide) : The amount of PICP) was measured. The results are shown in FIG.

1 is a graph showing the measurement of collagen biosynthesis of the composition according to the present invention. As shown here, the composition according to the present invention was confirmed to increase collagen production in human-derived fibroblasts, in particular, it was found that the extract fermentation is superior to the extract.

<Test Example 2> Determination of collagen degrading enzyme inhibitory effect

The effect of inhibiting collagen degrading enzyme was measured to observe the wrinkle improvement effect of the composition according to the present invention. Human normal fibroblasts were inoculated into 24-well microplates containing DMEM medium (2 × 10 ⁵ cells / well) and incubated at 37 ° C. for 24 hours in a CO ₂ incubator at 5% concentration. . After 24 hours, the medium was removed from each well and the samples prepared in Examples 1 and 2 were pretreated and UVA (5J / cm ² ) was incubated for 24 hours after treatment in each well after 2 hours. After 24 hours, cell media were collected to prepare samples.

The collagenase inhibitory effect was measured using a collagen degrading enzyme measurement kit (MMP-1 ELISA kit (QIA55), Merck, Germany) to measure the amount of collagenase according to the kit instructions. The results are shown in FIG.

Figure 2 is a graph showing the measurement of collagen degrading enzyme inhibitory effect of the composition according to the present invention. As shown here, the composition according to the present invention was confirmed to reduce the production of collagen degrading enzymes in human-derived fibroblasts, in particular, it was found that the extract fermentation is superior to the extract.

<Test Example 3> Wrinkle improvement effect measurement of the cosmetic

(Step 1) Preparation of nutrition cream

The composition of the nourishing cream containing the herbal fermented extract prepared in Examples 1 and 2 is as shown in Table 2 below. The aqueous phase purified water, triethanol amine and propylene glycol were heated and dissolved at 70 占 폚, and the oily fatty acid, oil component, emulsifier, and preservative were heated to 70 占 폚 to dissolve the solution. After emulsification was completed the solution was cooled to 45 ° C., extracted fermentation and flavor was added and dispersed and then cooled to 30 ° C.

ingredient Content (% by weight) Herbal Medicine Extract or Extracted Fermentation thereof 0.01, 0.05 or 1.00 Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Disteare 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Arginine 0.3 Carboxy Vinyl Polymer 0.25 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Remaining amount Sum 100

(Step 2) Wrinkle improvement effect of the cosmetics The wrinkle improvement effect of the cosmetics containing the medicinal herb fermentation extract was measured through clinical trials. The nutrient cream (Preparation Example 1 and Preparation Example 2) each containing 1% of the extract fermented product prepared in step 1 and the nutrient cream containing purified water were used as a comparative example.

Wrinkle improvement effect was evaluated by measuring skin elasticity change. Skin elasticity was measured in 30 healthy women (25 to 35 years old) under the condition that the temperature is maintained at a constant temperature of 24 to 26 ℃, humidity of 38 to 40% and three kinds of nutrition creams of Preparation Examples 1 and 2 The nutritional cream of the comparative example was applied to each subject's face twice a day for 3 months, and then measured by using a cutometer SEM 474 (Courage + Khazaka, Cologne, Germany). The relative values were compared with 0 and in many cases 5. The test results are shown in Table 3 below.

Test Items Preparation Example 1 Preparation Example 2 Comparative example Skin elasticity 2.68 2.87 2.31

As shown in Table 3, the cosmetics of Preparation Examples 1 and 2 of the present invention was much better wrinkle improvement effect than the cosmetics of the Comparative Preparation Example, in particular, it was confirmed that the extract-containing fermentation cosmetics have a very good skin elasticity. <Test Example 4> Wrinkle improvement effect measurement of food

(Step 1) Preparation of Drink

The beverage containing the herbal medicine fermented extract prepared in Examples 1 and 2 was prepared in a composition as shown in Table 4 below.

Raw material name  Compounding amount (g) ratio(%) Chinese Herb Extract Fermentation  1.0000 0.4125 Liquid fructose  1.0000  0.4125 Anhydrous Citric Acid  0.4000 0.1650 Fruit Blend 0.3241 0.1337 Refined salt 0.1081  0.0446 Vitamin c 0.1300 0.0536 Cooling scent 0.0550 0.0227 Potassium chloride  0.0400 0.0165 Sucralose 0.0150 0.0062 Magnesium chloride 0.0100 0.0041 Purified water  230.3180 95.0156 gun 242.40 100.0000

(Step 2) Wrinkle improvement effect of the beverage Wrinkle improvement effect of the beverage containing the observed drug extract fermentation was measured through clinical trials. The beverage containing the extracted fermented product prepared in the above step was ingested once daily for 4 weeks to three adult women aged 62, 61 and 55 years, and then the skin wrinkle improvement effect was measured.

3 to 5 is a clinical picture showing the wrinkle improvement effect of the beverage containing the herbal medicine extract fermentation according to the present invention. As shown here, it can be seen that after 4 weeks of ingestion of the beverage containing the herbal medicine extract fermentation according to the present invention, the skin wrinkles are significantly improved.

Test Example 5 Safety Confirmation on Human Skin

To confirm whether the herbal extract fermentation product is safe for human skin, skin safety verification experiments were performed. To this end, a cumulative skin irritation test was performed. Based on squalene, a formulation containing 1% of medicinal herb extract fermentation was prepared. Using this, a total of 9 healthy 24-hour cumulative patches on the upper forearm were performed every other day for 30 healthy adults. Whether or not this skin is irritated was measured. The patch method used the fin chamber (Finn chamber, Epitest Ltd, Finland). 15 µl of each of the external preparations for skin was added dropwise to the chamber, followed by patching. The degree of response to the skin each time was scored using Equation 1 below, and the results are shown in Table 5 below.

In the reactivity, ± gives 1 point, + gives 2 points, and ++ gives 4 points, which are judged as safe compositions when the average reactivity is less than 3.

Test substance Number of subjects with response Average
Responsiveness 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ Control
(Squalene) One - - 0 - - --- --- --- --- --- --- --- 0.09 extract
[Test Group 1] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 Extracted Fermentation
[Test Group 2] 0 - - 0 - - --- --- --- --- --- --- --- 0.00

In Table 5, the number of persons corresponding to ±, +, and ++ in test groups 1 and 2 was 0, 0, and 1, respectively, and the average reactivity was 0.00 and 0.00, respectively. Therefore, since the average reactivity shows a reactivity of 3 or less, the extracted fermentation product according to the present invention was determined to be a safe substance for human skin that does not exhibit a pronounced cumulative stimulation pattern. The preparation examples for the composition of the present invention are illustrated below.

<Example 1> Cosmetic preparation

1-1. Preparation of Soft Cosmetics

To a flexible skin lotion containing the herb extract fermentation as an active ingredient with a composition as shown in Table 6 was prepared according to a conventional method.

ingredient weight% Extracted Fermentation 0.01 glycerin 3.0 Butylene Glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 ethanol 10.0 Arginine 0.08 Preservatives, Micro Pigments, Micro-Fragrances and Micro-Purified Water 82.79 sum 100.0

1-2. Preparation of nutrient lotion A nutrient lotion containing a herb extract fermented product as an active ingredient with a composition as shown in Table 7 was prepared according to a conventional method.

ingredient weight% Herbal Medicine Extract or Extracted Fermentation 0.01 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene Glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Arginine 0.15 Preservatives, Micro Pigments, Micro-Fragrances and Micro-Purified Water 69.69 sum 100.0

1-3. Preparation of nutrition cream Nutritional cream containing a herbal extract extracted fermented product as an active ingredient as shown in Table 8 was prepared according to a conventional method.

ingredient weight% Herbal Medicine Extract or Extracted Fermentation 0.01 Beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 5.0 Butylene Glycol 3.0 Propylene glycol 3.0 Arginine 0.18 Preservatives, Micro Pigments, Micro-Fragrances and Micro-Purified Water 56.79 sum 100.0

1-4. Preparation of a massage cream A massage cream comprising a herbal extract fermented product as an active ingredient with a composition as shown in Table 9 was prepared according to a conventional method.

ingredient weight% Herbal Medicine Extract or Extracted Fermentation 0.01 Beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene Glycol 3.0 Propylene glycol 3.0 Arginine 0.2 Preservatives, Micro Pigments, Micro-Fragrances and Micro-Purified Water 27.49 sum 100.0

1-5. Preparation of a pack A pack including a herb extract fermentation as an active ingredient with a composition as shown in Table 10 was prepared according to a conventional method.

ingredient weight% Herbal Medicine Extract or Extracted Fermentation 0.01 Polyvinyl alcohol 13.0 Sodium Carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonylphenyl Ether 0.3 Preservatives, Micro Pigments, Micro-Fragrances and Micro-Purified Water 81.39 sum 100.0

Formulation Example 2 Preparation of Pharmaceutical Formulation 2-1. Manufacture of powder

In order to prepare a powder containing the herb extract fermentation as an active ingredient, the powder of the following Table 10 was mixed and then filled in an airtight cloth to prepare a powder.

ingredient g Herbal Medicine Extract or Extracted Fermentation 2 Lactose One

The above ingredients were mixed and filled in an airtight cloth to prepare a powder. Manufacture of tablets

In order to prepare tablets containing the herbal extract fermentation as an active ingredient, the tablets were prepared by mixing the components shown in Table 12 and then tableting according to the conventional method for preparing tablets.

ingredient mg Herbal Medicine Extract or Extracted Fermentation 100 Corn starch 100 Lactose 100 Magnesium Stearate 2

2-3. Preparation of Capsules In order to prepare a capsule containing the extract fermentation as an active ingredient, the ingredients of Table 13 were mixed and then filled into gelatin capsules according to the conventional capsule production method to prepare a capsule.

ingredient mg Herbal Medicine Extract or Extracted Fermentation 100 Corn starch 100 Lactose 100 Magnesium Stearate 2

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

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Claims (9)

Lactic acid bacteria Lactobacillus plantarum (L. plantarum ) in extracts of Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Conventional Fragrance and Placenta 4-4 weight based on the weight of the extract Cosmetic composition for preventing or improving skin wrinkles, characterized in that the fermentation by inoculating in an amount of% for 10 to 20 hours. The method of claim 1,
Sulfur yellow 2-10%, Astragalus 2-10%, Green tea 2-10%, Peony 2-10%, Cornus 1-8%, Brown root 1-8%, Schizandra 1-8%, Angelica Prevent or improve skin wrinkles, including 0.5 to 5% by weight, 0.5 to 5% by weight, cinnamon 0.5 to 5%, cinnamon 0.1 to 2%, placenta 1 to 3% and honey 40 to 60% Cosmetic composition for. The method of claim 1,
The extract is put into 10 times the amount (w / v) distilled water of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, geunyang and placenta weight, extracted at 90 ℃ for 4 hours and then filtered. Cosmetic composition for the prevention or improvement of skin wrinkles, characterized in that it is produced by drying under reduced pressure after collecting the supernatant three times. Lactic acid bacteria Lactobacillus plantarum (L. plantarum ) in extracts of Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Conventional Fragrance and Placenta 4-4 weight based on the weight of the extract Pharmaceutical composition for preventing or improving skin wrinkles, characterized in that fermented by inoculating in an amount of% for 10 to 20 hours incubation. The method of claim 6,
Sulfur yellow 2-10%, Astragalus 2-10%, Green tea 2-10%, Peony 2-10%, Cornus 1-8%, Brown root 1-8%, Schizandra 1-8%, Angelica Prevent or improve skin wrinkles, including 0.5 to 5% by weight, 0.5 to 5% by weight, cinnamon 0.5 to 5%, cinnamon 0.1 to 2%, placenta 1 to 3% and honey 40 to 60% Pharmaceutical composition for. The method of claim 6,
The extract is put into 10 times the amount (w / v) distilled water of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, geunyang and placenta weight, extracted at 90 ℃ for 4 hours and then filtered. A pharmaceutical composition for preventing or improving skin wrinkles, characterized in that it is prepared by drying under reduced pressure and then repeatedly collecting the supernatant three times. Lactic acid bacteria Lactobacillus plantarum (L. plantarum ) in extracts of Sukjihwang, Astragalus, Green tea, Peony, Cornus, Brown root, Schisandra chinensis, Angelica, Cnidium, Cinnamon, Conventional Fragrance and Placenta 4-4 weight based on the weight of the extract Food composition for preventing or improving skin wrinkles, characterized in that the fermentation by inoculating in an amount of% for 10 to 20 hours. The method of claim 11,
Sukji sulfur 2 to 10% by weight, Astragalus 2 to 10% by weight, green tea 2 to 10% by weight, peony 2 to 10% by weight, cornus oil 1 to 8% by weight, brown root 1 to 8% by weight, Schisandra chinensis 1 to 8% by weight, Angelica Prevent or improve skin wrinkles, including 0.5 to 5% by weight, 0.5 to 5% by weight, cinnamon 0.5 to 5%, cinnamon 0.1 to 2%, placenta 1 to 3% and honey 40 to 60% Food composition for. The method of claim 11,
The extract is put into 10 times the amount (w / v) distilled water of Sukjihwang, Astragalus, green tea, peony, cornus, brown root, Schisandra chinensis, Angelica, cheonggung, cinnamon, geunyang and placenta weight, extracted at 90 ℃ for 4 hours and then filtered. Food composition for preventing or improving the skin wrinkles, characterized in that it is prepared by drying under reduced pressure and then repeatedly collected three times to recover the supernatant.

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