KR102541810B1 - Compositions for improving skin comprising angelica and cranberry extracts and uses thereof - Google Patents

Abstract

The present invention relates to a composition for improving skin comprising angelica and cranberry extracts, and according to the composition according to one aspect, the composition is harmless to the human body and has a significantly improved skin condition improvement effect. For example, the composition has effects of strengthening the skin barrier, suppressing or alleviating skin aging, preventing or reducing skin wrinkles, or improving skin elasticity.

Description

Compositions for improving skin comprising angelica and cranberry extracts

It relates to a composition for improving skin comprising angelica and cranberry extracts.

Collagen is a major matrix protein produced by fibroblasts of the skin, and functions such as maintaining mechanical firmness of the skin and bonding strength of tissues, supporting cell adhesion, and inducing cell differentiation. Collagen is reduced by age and photoaging by ultraviolet irradiation, and collagen reduction is promoted by the activity of collagenase enzyme that decomposes collagen. It is known that it is closely related to the formation of wrinkles on the skin. In addition, it is known that collagen and elastin fibers play an important role in retaining moisture in the dermal layer and are involved in maintaining skin elasticity through skin moisturizing.

Currently, retinol, adenosine, chlorella extract, and the like are known as cosmetics for enhancing skin elasticity and improving wrinkles. Retinol is a substance that promotes collagen synthesis and inhibits the elastase enzyme, but is unstable and has safety issues such as irritation and rash when applied to the skin. It is known that it is difficult to expect a wrinkle improvement effect.

Therefore, it is necessary to discover natural materials that are safe for the human body while having excellent skin improvement effects.

Korea Patent Registration 10- 2442117

One aspect provides a cosmetic composition for improving skin condition comprising an Angelica archangelica extract and a cranberry extract.

Another aspect provides a health functional food composition for improving skin condition containing an angelica extract and a cranberry extract.

One aspect is to provide a cosmetic composition for improving skin condition comprising an Angelica archangelica extract and a cranberry extract.

As used herein, the term "Angelica ( Angelica archangelica )" is known as a biennial plant of the Apiaceae family, and may mean garden angelica, wild celery, or Norwegian angelica.

As used herein, the term "cranberry" may refer to the fruit of a bilberry tree belonging to the genus Vaccinium subgenus of the Azalea family Azalea family.

The extract may be extracted from at least one selected from the group consisting of angelica or cranberry sprouts, roots, stems, branches, leaves, seeds and fruits. Whole or part of the angelica or cranberry used for extraction, or materials derived therefrom, may be ground, minced, or dried.

The extraction method for obtaining the extract may be prepared according to known physical, chemical or enzymatic extraction methods commonly used in the art to which the present invention pertains. Specifically, commonly used extraction methods such as cold precipitation method, heat extraction method, ultrasonic extraction method, filtration method, pressure extraction method, reflux extraction method, supercritical extraction method, electrical extraction method, steam distillation method, organic solvent extraction method, and hot water extraction method may be used, and conventional extraction equipment may be used. , sonicating extractor or fractionator can be used.

In one embodiment, when the extract is extracted using a hot water extraction method, a solvent is added to the raw material and extracted at a temperature of 80 to 150 ° C. for 3 to 60 minutes, and then 15 to 25 ° C., for example, 16 to 25 ° C. ℃, 17 to 25 ℃, 17 to 24 ℃, 27 to 23 ℃, or may be to cool at room temperature. The hot water export method is useful for obtaining a large amount of extract in a short period of time, and the active ingredient content significantly increases as the extraction time and temperature increase. In addition, the extract may be used after lyophilizing the extract extracted using the hot water extraction method and then pulverizing it.

In one embodiment, the extract may be extracted using water, alcohol, or a mixture thereof as a solvent. The alcohol may be a C1 to C6 alcohol, for example a C1 to C4 alcohol. The C1 to C6 alcohol may be methanol, ethanol, propanol, isopropanol, 1,3-propanediol, butanol, pentanol, hexanol or butylene glycol.

In one embodiment, the extraction is 3 to 10 (volume or weight) times the extraction solvent for each extract, for example, 3 to 7 (volume or weight) times, 3 to 5 (volume or weight) times , 5 to 10 (volume or weight) times, or 4 to 10 (volume or weight) times.

In one embodiment, the solvent may be a mixture of water and alcohol, that is, an aqueous alcohol solution, for example, an aqueous ethanol solution. The alcohol concentration in the alcohol aqueous solution is 1 to 99.5 (v / v)%, 10 to 99.5 (v / v)%, 1 to 70 (v / v)%, 1 to 40 (v / v)%, 5 to 25 (v/v)%, 7 to 20 (v/v)%, 5 to 25 (v/v)%, or 10 to 20 (v/v)%.

In one embodiment, the skin condition improvement may be any one or more selected from the group consisting of strengthening skin barrier, inhibiting or improving skin aging, preventing or improving skin wrinkles, and improving skin elasticity.

As used herein, the term "skin barrier improvement" may refer to any action that enhances the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients.

As used herein, the term "skin aging" may mean both tangible and intangible changes that occur in the skin with age. For example, thinning of the epidermis, the number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle, wound healing, skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical It can mean a decrease in damage defense, chemical elimination ability, immune response, sensory function, and thermoregulation.

The skin aging specifically includes early aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemicals, etc., as well as natural aging caused by the decrease in the proliferation of skin cells with age. It can include wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles may include inducing wrinkles by changing components constituting skin tissue due to stimulation caused by changes in internal and external factors.

The aging may be photoaging. The term "photoaging" is a phenomenon caused by external environmental factors, and may be caused by ultraviolet rays.

As used herein, the term "wrinkle" may refer to a state in which the elasticity of the skin is lost and loosened, and for example, the skin may be folded. Pigmentation may mean a condition in which the amount of pigment in the body is abnormal or there is an abnormality in the place where the pigment appears, and may include, for example, melasma and freckles. The "prevention or improvement of skin wrinkles" may mean any action of preventing or improving wrinkles by suppressing the expression of wrinkle-related factors or increasing the total amount of collagen.

Therefore, the cosmetic composition promotes the expression or activity of COL1A1 (collagen, type I, alpha 1) or elastin, or inhibits the expression or activity of MMP1 (Matrix metalloproteinase-1), thereby improving skin conditions. there may be

The content of the cranberry extract may be equal to or less than the content of the angelica extract.

According to one embodiment, the cosmetic composition contains angelica extract and cranberry extract in a weight ratio of 10 to 75: 1, for example, 10 to 70: 1, 10 to 65: 1, 10 to 60: 1, 10 to 55: 1, 10 to 50:1, 15 to 75:1, 15 to 70:1, 15 to 65:1, 15 to 60:1, 15 to 55:1, 15 to 50:1, 20 to 75:1, 20 to 70:1, 20 to 65:1, 20 to 60:1, 20 to 55:1, 20 to 50:1, 25 to 75:1, 25 to 70:1, 25 to 65:1, 25 to 60:1, 25 to 55:1, 25 to 50:1, 30 to 75:1, 30 to 70:1, 30 to 65:1, 30 to 60:1, 30 to 55:1, 30 to 50: 1, 35 to 75:1, 35 to 70:1, 35 to 65:1, 35 to 60:1, 35 to 55:1, 35 to 50:1, 40 to 75:1, 40 to 70:1, 40 to 65:1, 40 to 60:1, 40 to 55:1, 40 to 50:1, 45 to 75:1, 45 to 70:1, 45 to 65:1, 45 to 60:1, 45 to It may be included in a weight ratio of 55: 1, or 45 to 50: 1.

The angelica extract is 0.1 to 1% by weight, for example, 0.1 to 0.9% by weight, 0.1 to 0.8% by weight, 0.1 to 0.7% by weight, 0.1 to 0.6% by weight, 0.1 to 0.5% by weight, 0.1 to 0.5% by weight, based on the total weight of the cosmetic composition to 0.4 wt%, 0.1 to 0.3 wt%, 0.1 to 0.2 wt%, 0.2 to 1 wt%, 0.2 to 0.9 wt%, 0.2 to 0.8 wt%, 0.2 to 0.7 wt%, 0.2 to 0.6 wt%, 0.2 to 0.5 wt% % by weight, 0.2 to 0.4% by weight, 0.2 to 0.3% by weight, 0.3 to 1% by weight, 0.3 to 0.9% by weight, 0.3 to 0.8% by weight, 0.3 to 0.7% by weight, 0.3 to 0.6% by weight, 0.3 to 0.5% by weight , 0.3 to 0.4% by weight, 0.4 to 1% by weight, 0.4 to 0.9% by weight, 0.4 to 0.8% by weight, 0.4 to 0.7% by weight, 0.4 to 0.6% by weight, 0.4 to 0.5% by weight, 0.5 to 1% by weight, 0.5 to 0.9 wt%, 0.5 to 0.8 wt%, 0.5 to 0.7 wt%, 0.5 to 0.6 wt%, 0.6 to 1 wt%, 0.6 to 0.9 wt%, 0.6 to 0.8 wt%, 0.6 to 0.7 wt%, 0.7 to 1 % by weight, 0.7 to 0.9% by weight, 0.7 to 0.8% by weight, 0.8 to 1% by weight, 0.8 to 0.9% by weight, 0.9 to 1% by weight, 0.1 to 0.98% by weight, 0.2 to 0.98% by weight, 0.3 to 0.98% by weight , 0.4 to 0.98% by weight, 0.5 to 0.98% by weight, 0.6 to 0.98% by weight, 0.7 to 0.98% by weight, 0.8 to 0.98% by weight, or 0.9 to 0.98% by weight.

The cranberry extract is 0.01 to 0.03% by weight relative to the total weight of the cosmetic composition, for example, 0.01 to 0.025% by weight, 0.01 to 0.02% by weight, 0.01 to 0.015% by weight, 0.015 to 0.03% by weight, 0.015 to 0.025% by weight, 0.015 to 0.02% by weight, 0.02 to 0.03% by weight, 0.02 to 0.025% by weight, or 0.025 to 0.03% by weight.

The cosmetic composition may be prepared in any formulation commonly prepared in the art to which the present invention pertains. For example, it may be formulated into softening lotion, nutrient lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc. It is not limited. More specifically, skin lotion, skin softener, skin toner, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulations.

The cosmetic composition may include additional ingredients commonly used in cosmetics, such as thickeners, dispersants, fragrances, fillers, preservatives, preservatives, humectants, neutralizers, sweeteners, vitamins, free-radical scavengers, metal ion sequestering agents, and functional ingredients. and any conventional cosmetic ingredient which may be selected from mixtures thereof. One skilled in the art may select any additional ingredients and/or amounts thereof such that the beneficial properties of the compositions according to the present disclosure are not adversely affected or substantially unaffected by the anticipated addition.

The preservative may include 1,2-hexanediol, caprylyl glycol, hydroxyacetophenone, ethylhexyl glycerin, phenoxyethanol, pentylene glycol, or a combination thereof, but is not limited thereto.

The humectant may include 1,3-propanediol, butylene glycol, glycerin, methylpropanediol, diglycerin, isopentyldiol, polyglycerin-3, or a combination thereof, but is not limited thereto. .

When the cosmetic composition is a surfactant-containing cleansing formulation, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester may be further included.

When the cosmetic composition is a cream or gel formulation, it may further include animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component.

When the cosmetic composition is a solution or emulsion formulation, solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters and the like may be further included.

When the cosmetic composition is a suspension formulation, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may further be included.

When the cosmetic composition is in a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, additionally chlorofluorohydrocarbon , a propellant such as propane/butane or dimethyl ether.

The cosmetic composition may be used by applying alone or overlapping, or by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

Another aspect provides a health functional food composition for improving skin conditions including an Angelica archangelica extract, a cranberry extract, or a combination thereof.

The angelica extract, cranberry extract, and skin conditions were as described above.

According to one embodiment, the health functional food composition contains angelica extract and cranberry extract in a weight ratio of 10 to 75: 1, for example, 10 to 70: 1, 10 to 65: 1, 10 to 60: 1, 10 to 55:1, 10 to 50:1, 15 to 75:1, 15 to 70:1, 15 to 65:1, 15 to 60:1, 15 to 55:1, 15 to 50:1, 20 to 75: 1, 20 to 70:1, 20 to 65:1, 20 to 60:1, 20 to 55:1, 20 to 50:1, 25 to 75:1, 25 to 70:1, 25 to 65:1, 25 to 60:1, 25 to 55:1, 25 to 50:1, 30 to 75:1, 30 to 70:1, 30 to 65:1, 30 to 60:1, 30 to 55:1, 30 to 50:1, 35 to 75:1, 35 to 70:1, 35 to 65:1, 35 to 60:1, 35 to 55:1, 35 to 50:1, 40 to 75:1, 40 to 70: 1, 40 to 65: 1, 40 to 60: 1, 40 to 55: 1, 40 to 50: 1, 45 to 75: 1, 45 to 70: 1, 45 to 65: 1, 45 to 60: 1, It may be included in a weight ratio of 45 to 55: 1, or 45 to 50: 1.

The health functional food composition may be used together with other food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of health functional food. Among the types of health functional foods, beverage compositions may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The health food composition may also contain nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonated beverages. carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverages, fruit flesh for preparing vegetable beverages, or a combination thereof.

The extract is 0.0001 to 99.9999% by weight, 0.0001 to 90% by weight, 0.0001 to 50% by weight, 0.0001 to 10% by weight, 0.01 to 99.9999% by weight, 0.01 to 90% by weight, 0.01 to 50% by weight based on the total weight of the health functional food %, 0.01 to 10% by weight, 0.1 to 99.9999% by weight, 0.1 to 90% by weight, 0.1 to 50% by weight, or 0.1 to 10% by weight, but may be included, but is not particularly limited thereto.

According to the composition according to one aspect, there is a significantly improved skin condition improvement effect harmless to the human body. For example, there is an effect of strengthening the skin barrier, suppressing or improving skin aging, preventing or improving skin wrinkles, or improving skin elasticity.

1 is an image showing a criterion for the degree of discoloration for determining the long-term stability of a formulation.

It will be described in more detail through the following examples. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

Example 1. Preparation of Angelica Extract

In order to prepare the angelica extract, raw angelica was collected and extracted under high temperature conditions through a solvent extraction method. Specifically, purified water, butylene glycol, and a solvent containing 1,2-hexanediol were added to Angelica archangelica, followed by extraction at 80 to 85 °C for 2 hours. Then, after performing primary filtration using a filter having a diameter of 0.8 μm, secondary filtration was performed using a filter having a diameter of 0.2 μm to prepare an angelica extract.

Example 2. Preparation of cranberry extract

To prepare a cranberry extract, cranberry raw material was collected and extracted under high temperature conditions through a solvent extraction method. Specifically, after stabilizing cranberry raw material (Vaccinium Macrocarpon) in water, enzymatic hydrolysis was performed, and then the enzyme was inactivated through heat treatment. Thereafter, the soluble and insoluble phases were separated, and after purification and decolorization by ion chromatography, a stabilizer including 1,2-hexanediol and caprylyl glycol was further added, followed by filtration to prepare a cranberry extract.

Preparation Examples 1 to 8. Preparation of compositions containing angelica extract and cranberry extract.

In order to prepare a composition containing the angelica extract and cranberry extract prepared in Examples 1 and 2, the ingredients in Table 1 below were mixed. Specific ingredients and contents are shown in Table 1 below in weight%.

- control group Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Preservative (1,2-hexanediol)
One One One One One One One One One Water-soluble humectants (butylene glycol and glycerin) 3 3 3 3 3 3 3 3 3 Angelica Extract - 0.01 0.1 0.5 0.98 0.5 1.0 0.5 - cranberry extract - 0.001 0.01 0.02 0.025 0.03 0.02 - 0.02 Weight Ratio (Angelica Extract : Cranberry Extract) - 10:1 10:1 25:1 39:1 16:1 50:1 - -

Experimental Example 1. Confirm anti-wrinkle effect of a composition containing angelica extract and cranberry extract

In order to confirm the anti-wrinkle effect of Preparation Examples 1 to 8, mRNA expression of COL1A1 (collagen type 1, alpha 1) and elastin according to the treatment of Preparation Example in normal human dermal fibroblasts (NHDFs) The amount was confirmed by real-time PCR.

First, in order to set the concentration of the test solution of the preparation example, human dermal fibroblasts were treated with the preparation example for 24 hours, and the concentration range in which cytotoxicity was not observed was confirmed through a cytotoxicity assay. In addition, human dermal fibroblasts were treated with concentrations of test substances that were not toxic, and the expression levels of COL1A1 mRNA, a collagen gene, and Elastin mRNA, an elastin gene, were compared and analyzed through Quantitative Real-Time PCR (qRT-PCR) method. did

And the expression level of each preparation example is shown in Table 2 based on the expression level of the control group.

- control group Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 COL1A1 mRNA 100% 100% 117% 141% 389% 152% 312% 130% 115% Elastin mRNA 100% 101% 172% 191% 279% 170% 280% 154% 127%

As shown in Table 2, it was confirmed that the composition containing the angelica extract and the cranberry extract significantly improved the expression of COL1A1 and elastin compared to the control group.

Specifically, it was confirmed that the expression of COL1A1 and elastin was effectively improved compared to the control group when the angelica extract and the cranberry extract were included at a constant content.

More specifically, when the angelica extract and the cranberry extract were included at a weight ratio of 10 to 75: 1, it was confirmed that the expression of COL1A1 and elastin significantly improved compared to Preparation Example 7 or 8 containing only the angelica extract or the cranberry extract.

In particular, Preparation Examples 4 and 6 containing angelica extract and cranberry extract at a weight ratio of 30 to 75: 1 confirmed that the expression level of COL1A1 and elastin was about 3 times higher than that of the control group, and angelica extract or cranberry It was confirmed that the expression level was about 2.5 times higher than that of Preparation Example 7 or 8 containing only the extract.

The above results mean that the composition containing the angelica extract and the cranberry extract has a significantly improved skin condition improvement effect compared to the composition containing only the angelica extract or the cranberry extract.

Experimental Example 2. Measurement of Discoloration Degree of Composition Containing Angelica Extract and Cranberry Extract

In order to confirm the long-term stability of Preparation Examples 1 to 8, Preparation Examples 1 to 8 were stored at high temperature (50 ° C.) for 1 month, and then the degree of discoloration of the formulation was confirmed. And the results are shown in Table 3.

control group Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 degree of discoloration O 0 2 3 3 4 4 One 3

※ Formulation discoloration degree (0 ~ 5): The greater the degree of discoloration, the higher the number, and the specific judgment is shown in FIG. 1.

As shown in Table 3, as a result of storage for 1 month under high temperature (50 ° C.) conditions, it was confirmed that the degree of discoloration of the formulations in Preparation Examples 5 and 6 was large. On the other hand, in the case of Preparation Examples 1 to 4, 7 and 8, it was confirmed that the degree of discoloration of the formulation was not large. The above results indicate that when the angelica extract or the cranberry extract is included in a certain amount, the discoloration problem of the formulation can be effectively prevented and the long-term stability can be significantly improved.

Claims (6)

Angelica ( Angelica archangelica ) As a cosmetic composition for improving skin condition containing an extract and cranberry (cranberry) extract;
The cranberry extract is included in an amount of 0.01 to 0.025% by weight relative to the total weight of the composition, and the composition includes an angelica extract and a cranberry extract in a weight ratio of 30 to 75: 1,
The skin condition improvement is any one or more selected from the group consisting of strengthening skin barrier, inhibiting or improving skin aging, preventing or improving skin wrinkles, and improving skin elasticity, the cosmetic composition. The method of claim 1,
The angelica extract is contained in 0.1 to 0.98% by weight relative to the total weight of the composition, the cosmetic composition. delete delete delete The method of claim 1,
The composition promotes the expression or activity of COL1A1 or elastin, a cosmetic composition.

Images