KR20170136375A - Cosmetic compositions of the formulation forming transparent skin protective film - Google Patents

Cosmetic compositions of the formulation forming transparent skin protective film Download PDF

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KR20170136375A
KR20170136375A KR1020160068385A KR20160068385A KR20170136375A KR 20170136375 A KR20170136375 A KR 20170136375A KR 1020160068385 A KR1020160068385 A KR 1020160068385A KR 20160068385 A KR20160068385 A KR 20160068385A KR 20170136375 A KR20170136375 A KR 20170136375A
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skin
cosmetic composition
atelocollagen
protective film
present
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KR1020160068385A
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Korean (ko)
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배수현
정진아
유지철
서동삼
장정호
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세원셀론텍(주)
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Priority to KR1020160068385A priority Critical patent/KR20170136375A/en
Priority to CN201680086243.9A priority patent/CN109195581A/en
Priority to PCT/KR2016/006304 priority patent/WO2017209334A1/en
Priority to JP2018562037A priority patent/JP2019516772A/en
Publication of KR20170136375A publication Critical patent/KR20170136375A/en

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/731Cellulose; Quaternized cellulose derivatives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/26Optical properties
    • A61K2800/262Transparent; Translucent
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/28Rubbing or scrubbing compositions; Peeling or abrasive compositions; Containing exfoliants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Emergency Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The present invention relates to a cosmetic composition of a formulation forming a transparent skin protective barrier. More specifically, the present invention relates to a cosmetic composition, which contains 0.01-1.0 wt% of a medical grade of atelocollagen relative to the total weight of the cosmetic composition to form a transparent skin protective barrier, thereby providing effects of moisturizing the skin and reducing dead skin cells. The cosmetic composition is suitable as a skin tonic (skin lotion) formulation due to low viscosity and light feeling on the skin.

Description

투명 피부 보호막을 형성하는 제형의 화장료 조성물 {COSMETIC COMPOSITIONS OF THE FORMULATION FORMING TRANSPARENT SKIN PROTECTIVE FILM}BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cosmetic composition for forming a transparent skin protective film,

본 발명은 투명 피부 보호막을 형성하는 제형의 화장료 조성물에 관한 것이다. 보다 상세하게는 의료등급의 아텔로콜라겐을 함유함으로써 투명 피부 보호막을 형성하고, 이로 인해 피부 보습 및 각질 개선 효과를 제공하는 화장료 조성물에 관한 것이다.The present invention relates to cosmetic compositions of formulations which form a clear skin barrier. More particularly, the present invention relates to a cosmetic composition which comprises a medical grade of Atelocollagen to form a transparent skin protective film, thereby providing a skin moisturizing and exfoliation improving effect.

콜라겐(collagen)은 동물의 뼈, 연골, 이, 건, 피부 등에 있는 경단백질이고, 교원질이라고도 한다. 이는 섬유상 고체로 존재하고, 복잡한 가로무늬 구조로 되어있다. 생체내의 거의 모든 조직에 분포하는 콜라겐은 세포의 지지, 증식을 위한 구조체로써 주로 결합조직의 세포에 결합하여 장기와 조직을 형성하는 단백질이다. 이러한 콜라겐의 종류는 각 조직마다 다르며, 특히 제 1 형 콜라겐은 피부, 인대, 골 등 거의 모든 조직에 다량 포함되어 있기 때문에 조직공학에서 가장 널리 이용되고 있다. 또한, 제 1 형 콜라겐 분자의 양쪽 말단에는 나선을 형성하지 않은 텔로펩타이드(telopeptide)가 존재하며, 이는 면역반응을 일으키는 주된 원인이다. 아텔로콜라겐(atelocollagen)은 이 텔로펩타이드가 효소에 의해 제거되며 생성되는 단백질 유도체이며, 특유의 삼중 나선구조로 이상적인 생체적합성을 지니므로 의약품이나 화장품 등의 원료로 사용된다.Collagen is a light protein in animal bones, cartilage, teeth, skins, and skin, also called collagen. It is present as a fibrous solid and has a complex horizontal pattern structure. Collagen, which is distributed in almost all tissues in living body, is a structure for support and proliferation of cells, and is a protein that binds to cells of connective tissue to form organs and tissues. These types of collagen vary from tissue to tissue, and type 1 collagen is most widely used in tissue engineering because it is abundant in almost all tissues including skin, ligament, and bone. In addition, telopeptides without helices are present at both ends of the type 1 collagen molecule, which is a major cause of the immune response. Atelocollagen is a protein derivative that is produced by enzymatic cleavage of this telopeptide. It has a unique triple helix structure and is ideal biocompatibility and therefore used as a raw material for medicines and cosmetics.

피부는 표피, 진피, 피하지방 조직의 3개 층으로 구분되며, 외부환경에 직접 노출되어 있어 다양한 외부적 요인으로부터 방어 기능을 수행하는 조직이다. 특히 피부는 인체가 갖고 있는 약 65~70%의 수분이 체외로 증발되는 것을 조절해 준다. 그 중에서도 표피는 외부로부터 각질층, 과립층, 유극층, 기저층의 순서로 구분되는데, 표피의 각질층은 약 10~20%의 수분을 함유하고 인체의 최외각에 존재하여 체내로부터의 수분 증발을 억제한다. 피부 보습 정도는 화장품 분야에서 중요한 요소로, 피부의 외형(피부의 유연성 및 거친 정도)과 직접적으로 연관되어 있다. 또한, 각질 개선 정도는 피부의 컨디션 및 투과성과 관련되는 피부 막 항상성에 영향을 미친다.The skin is divided into three layers: epidermis, dermis, and subcutaneous fat tissue. It is directly exposed to the external environment and performs defense functions from various external factors. In particular, skin controls the evaporation of about 65-70% of the human body's moisture. Among them, the epidermis is divided into stratum corneum, granular layer, polar layer, and basal layer from the outside. The stratum corneum of the epidermis contains about 10 to 20% of water and exists in the outermost part of the human body to suppress water evaporation from the body. The degree of skin moisturization is an important factor in the field of cosmetics and is directly related to the appearance of the skin (the flexibility and roughness of the skin). In addition, the degree of skin exfoliation affects skin membrane homeostasis associated with skin condition and permeability.

최근 일반적인 화장료의 사용보다 피부에 물리 화학적인 효능·효과를 나타내는 화장료에 관한 관심이 증가하면서 이에 대한 연구가 활발히 진행되고 있다. 특히 피부 보호막을 형성하여 피부를 보호하고 수분함량을 늘려주는 화장품에 대한 관심이 증대되고 있다. 그러나 이러한 화장품은 팩, 크림 등의 점도가 있는 제형에 국한되어왔다.Recently, interest in cosmetics, which exhibit physiochemical effects and effects on the skin, has been more actively studied than in general cosmetics. Especially, there is an increasing interest in cosmetics that protect skin and form moisture barrier by forming a skin barrier. However, these cosmetics have been confined to formulations having viscosities such as packs, creams, and the like.

이에 본 발명자들은 가벼운 사용감을 가지고, 의료등급의 아텔로콜라겐을 함유함으로써 투명 피부 보호막을 형성하고, 이로 인해 보습 및 각질 개선 효과를 제공하는 화장료 조성물을 제조하였다. 본 발명에서 사용된 아텔로콜라겐은 특허등록 제 10-0676285호(명칭 : 동물의 다양한 조직으로부터의 콜라겐 분리방법 및 콜라겐 용액의 제조방법 그리고 이를 이용하여 생산한 매트릭스)에 따른 방법으로 생산된 의료등급의 원료를 사용하였으며, 이의 보습 및 각질 개선 효과는 특허등록 제 10-0924643호(명칭 : 아텔로콜라겐을 포집한 리포좀의 제조방법)에 의해 입증된 바 있다.Thus, the present inventors have produced a cosmetic composition which has a light feeling of use, contains a medical grade of Atelocollagen to form a transparent skin protective film, and thereby provides a moisturizing and exfoliation improving effect. The atelocollagen used in the present invention is a medical grade produced by the method according to Patent Registration No. 10-0676285 (name: a method for separating collagen from various tissues of animals and a method for producing collagen solution and a matrix produced using the same) , And its moisturizing and exfoliation improving effect has been proved by Patent Registration No. 10-0924643 (name: a method for producing liposome capturing atelocollagen).

종래 선행기술문헌으로는 특허등록 제 10-0285396호(명칭 : 복합안료, 이의 제조방법 및 이를 함유하는 화장료)가 등록된 바 있다.As a prior art document, Patent Registration No. 10-0285396 (name: a composite pigment, a method for producing the same, and a cosmetic containing the same) has been registered. 즉, 상기한 종래의 기술은 아텔로콜라겐, 히아루론산 등을 함유하여 피부 보습 효과를 증대시키고 주름형성을 억제하는 등의 효과를 갖는 크림과 같은 화장료 조성물을 제조하는 방법을 제공하였다. 그러나 상기한 종래의 기술에서 아텔로콜라겐은 보습제 및 필링재로써 사용되었으며, 본 발명의 아텔로콜라겐은 투명 피부 보호막을 형성하는 제형을 구현하기 위한 용도로 사용되었다. 그러므로 본 발명의 조성물 제조 방법은 상기한 종래의 기술과 상이한 별개의 발명이다.That is, the above-mentioned conventional technique has provided a method for producing a cosmetic composition such as a cream, which contains atelocollagen, hyaluronic acid and the like to increase skin moisturizing effect and suppress wrinkle formation. However, in the above conventional arts, atelocollagen has been used as a moisturizing agent and a filler, and the atelocollagen of the present invention has been used for realizing a formulation for forming a transparent skin barrier. Therefore, the method for producing a composition of the present invention is a separate invention different from the above-described conventional techniques.

본 발명은 의료등급의 아텔로콜라겐을 함유함으로써 투명 피부 보호막을 형성하는 제형의 화장료 조성물에 관한 것이다. 또한 본 발명에 의한 조성물은 투명 피부 보호막에 의해 피부 보습 및 각질 개선 효과를 제공할 수 있다.The present invention relates to a cosmetic composition of a formulation which forms a transparent skin barrier by containing medical grade atelocollagen. In addition, the composition of the present invention can provide skin moisturizing and exfoliation improving effect by a transparent skin protective film.

본 발명은 아텔로콜라겐을 함유하는 화장료 조성물로써, 투명 피부 보호막을 형성하는 것을 특징으로 하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition containing atelocollagen, which comprises a transparent skin protective film.

상기에서 설명한 바와 같이, 본 발명의 목적은 의료등급의 아텔로콜라겐을 함유하여 투명 피부 보호막을 형성하는 제형의 화장료 조성물을 제공하는 것으로써, 피부에 보습 및 각질 개선 효과를 갖는 화장료 조성물을 제공하는 것이며, 상기 화장료 조성물은 점도가 낮아 사용감이 가벼운 화장수(스킨로션) 제형에 적합하다.As described above, it is an object of the present invention to provide a cosmetic composition containing a medical grade atelocollagen to form a transparent skin protective film, thereby providing a cosmetic composition having moisturizing and exfoliation improving effect on the skin , And the cosmetic composition is suitable for a cosmetic lotion (skin lotion) formulation having a low viscosity due to its low feeling of use.

도 1은 본 발명에 따른 실시예 1의 상온 건조 후 제형 사진(A : 실시예 1 제형의 정면, B : 실시예 1 제형의 투명도, C : 실시예 1 제형의 단면)
도 2는 본 발명에 따른 실시예 1 및 비교예 1~4의 상온 건조 후 투명막 형성 양상 관찰 사진(A~E : 실시예 1 및 비교예 1~4 제형의 육안 관찰, F~J : 실시예 1 및 비교예 1~4 제형의 현미경 관찰(200 배율), K~O : 실시예 1 및 비교예 1~4 제형의 콜라겐 발색 육안 관찰)
도 3은 본 발명에 따른 실시예 1 및 비교예 3의 SDS-PAGE 패턴(M : SDS-PAGE 표준 마커, 1 : 실시예 1, 2 : 비교예 3)
도 4는 본 발명에 따른 실시예 1의 도포 전, 후 피부 수분량 변화를 측정한 결과
도 5는 본 발명에 따른 실시예 1의 도포 전, 후 피부 각질 지수를 측정한 결과
Fig. 1 is a photograph of a formulation after drying at room temperature according to the present invention (A: front view of the formulation of Example 1, B: transparency of the formulation of Example 1, C:
FIG. 2 is a photograph (A to E) of the transparent film formation after drying at room temperature of Example 1 and Comparative Examples 1 to 4 according to the present invention (visual observation of Form 1 and Comparative Examples 1 to 4, F to J) Microscope observation (200 magnification) of the formulation of Example 1 and Comparative Examples 1 to 4, K to O: visual observation of the collagen color of the formulation of Example 1 and Comparative Examples 1 to 4)
3 shows the results of SDS-PAGE (M: SDS-PAGE standard marker, 1: Examples 1 and 2: Comparative Example 3) of Example 1 and Comparative Example 3 according to the present invention.
Fig. 4 is a graph showing changes in skin moisture content before and after application of Example 1 according to the present invention
FIG. 5 is a graph showing the skin keratin index before and after application of Example 1 according to the present invention

본 발명의 화장료 조성물은 피부 보호막을 형성하여 피부를 보호하고 수분함량을 늘려주면서도 점도가 낮아 가벼운 사용감을 갖기 위하여 의료등급의 아텔로콜라겐을 화장료 조성물의 총 중량에 대하여 0.01 내지 1.0 중량%로 함유하는 것을 특징으로 한다. 이 때, 아텔로콜라겐의 중량이 조성물의 총 중량에 대해 0.01 중량% 미만인 경우에는 투명 피부 보호막을 고르게 형성하기 어려우며, 1.0 중량%를 초과할 경우에는 화장품 제조 시 혼합이 어려운 문제점이 있다.The cosmetic composition of the present invention forms a skin protective film to protect skin and increase the water content, while having a low viscosity to have a light feeling, it is preferable that atelocollagen of medical grade is contained in an amount of 0.01 to 1.0% by weight based on the total weight of the cosmetic composition . At this time, when the weight of atelocollagen is less than 0.01% by weight based on the total weight of the composition, it is difficult to uniformly form a transparent skin protective film. When the weight exceeds 1.0% by weight, mixing is difficult.

또한 본 발명의 화장료 조성물은 아텔로콜라겐을 함유함으로써 투명 피부 보호막을 형성하는 것을 특징으로 한다.Further, the cosmetic composition of the present invention is characterized by forming a transparent skin protective film by containing atelocollagen.

또한 본 발명의 화장료 조성물은 투명 피부 보호막 형성에 의해 피부 보습 및 각질 개선 효과를 갖는 것을 특징으로 한다.Further, the cosmetic composition of the present invention is characterized by having a skin moisturizing and exfoliation improving effect by forming a transparent skin protective film.

한편, 본 발명에서 투명 피부 보호막의 의미는 아텔로콜라겐이 함유된 화장료가 형성하는 투명한 막이라는 뜻으로, 구체적으로 피부 보습 및 각질 개선 효과를 가지며 의료등급 아텔로콜라겐을 가지는 화장료가 형성하는 투명한 막이라고 말할 수 있다.Meanwhile, in the present invention, the transparent skin protective film means a transparent film formed by a cosmetic composition containing atelocollagen. Specifically, the transparent skin protective film has a skin moisturizing and exfoliation improving effect, .

본 발명은 다양한 변경을 가할 수 있고 특정 실시예 및 시험예를 가질 수 있는 바, 이를 도면에 예시하고 구체적으로 설명하고자 한다. 그러나 발명이 반드시 이에 한정되는 것은 아니다.The present invention can be variously modified and can have specific examples and test examples, which are illustrated and specifically described in the drawings. However, the invention is not necessarily limited thereto.

[실시예 1 및 비교예 1~4][Example 1 and Comparative Examples 1 to 4]

표 1에 표시된 성분에 따라 실시예 1 및 비교예 1~4의 화장료 조성물을 제조하였다. 하기의 단위는 중량%이다.The cosmetic compositions of Example 1 and Comparative Examples 1 to 4 were prepared according to the ingredients shown in Table 1. The following units are by weight.

실시예 1 및 비교예 1~4의 화장료 성분 (단위 : 중량%)The cosmetic ingredients (unit:% by weight) of Example 1 and Comparative Examples 1 to 4
성 분 명

Name

실시예

Example

비교예

Comparative Example

1

One

1

One

2

2

3

3

4

4

정제수

Purified water

87.815

87.815

-

-

90.000

90,000

99.000

99,000

99.900

99,900

부틸렌글라이콜

Butylene glycol

5.000

5.000

-

-

-

-

-

-

-

-

글리세린

glycerin

4.000

4.000

-

-

-

-

-

-

-

-

1,2-헥산디올

1,2-hexanediol

1.500

1.500

-

-

-

-

-

-

-

-

아텔로콜라겐

Atelocollagen

1.000

1,000

100.000

100,000

10.000

10,000

1.000

1,000

0.100

0.100

셀룰로오스검

Cellulose sword

0.400

0.400

-

-

-

-

-

-

-

-

소듐 하이알루로네이트

Sodium hyaluronate

0.100

0.100

-

-

-

-

-

-

-

-

소듐락테이트

Sodium lactate

0.025

0.025

-

-

-

-

-

-

-

-

틱애씨드

Tic Acid

0.160

0.160

-

-

-

-

-

-

-

-

실시예 1은 투명 피부 보호막을 형성할 수 있는 화장료 조성물의 제조 성분이며, 이는 루시드 젤로 명명하였다. 도 1에 도시된 바와 같이 실시예 1은 상온 건조 시 투명 피부 보호막을 형성함을 알 수 있다.Example 1 is a manufacturing component of a cosmetic composition capable of forming a transparent skin protective film, which is named lucid gel. As shown in FIG. 1, Example 1 shows that a transparent skin protective film is formed at room temperature drying.

시험예Test Example 1.  One. 아텔로콜라겐의Atelocollagen 적정 혼합 함량 측정 Determination of Proper Mixed Content

투명 피부 보호막을 형성할 수 있는 화장료 조성물의 아텔로콜라겐 혼합 함량을 확인하기 위하여 상기 표 1에 표시된 비율로 실시예 1 및 비교예 1~4를 제조하여 막 형성의 양상을 관찰하였고, 도 2에 결과를 표시하였다.In order to confirm the content of the atelocollagen mixture in the cosmetic composition capable of forming a transparent skin protective film, Example 1 and Comparative Examples 1 to 4 were produced at the ratios shown in Table 1, Results are shown.

실시예 1 및 비교예 1~4를 Ø50 ×10 mm 페트리디쉬에 5.0 ±0.1 g씩 분주하여 상온(25℃) 건조한 후, 콜라겐 발색시약(sircol dye reagent)을 1 mL씩 처리하여 5 분간 반응시키고, 발색시약을 모두 제거한 뒤 각 시료의 색 변화를 관찰하였다.5.0 and 0.1 g of each of Example 1 and Comparative Examples 1 to 4 were dispensed into a Ø50 × 10 mm Petri dish and dried at room temperature (25 ° C.). Then, 1 mL of a collagen coloring reagent was treated for 5 minutes, , Color development reagents were all removed and color change of each sample was observed.

그 결과, 도 2에 도시된 바와 같이 실시예 1과 비교예 1~3의 조성물은 표면이 균일한 투명 막을 형성하며, 아텔로콜라겐이 페트리디쉬에 고르게 퍼지는 양상을 보였다. 비교예 4의 조성물은 투명 막의 형성 유무를 육안으로 확인하기 어려웠으며, 아텔로콜라겐이 페트리디쉬 가장자리에 분포하는 양상을 보였다. 이를 통해 아텔로콜라겐이 0.01 내지 1.0 중량% 농도 범위에서 실시예 1의 투명 피부 보호막 형성에 영향을 미칠 수 있음을 알 수 있었다.As a result, as shown in FIG. 2, the composition of Example 1 and Comparative Examples 1 to 3 formed a transparent film with uniform surface, and showed a state in which atelocollagen spread evenly in the petri dish. In the composition of Comparative Example 4, it was difficult to visually confirm whether a transparent film was formed or not, and atelocollagen was distributed at the edge of the petri dish. As a result, it was found that atelocollagen can affect the formation of the transparent skin protective film of Example 1 in the concentration range of 0.01 to 1.0 wt%.

또한 SDS-PAGE 결과, 도 3에 도시된 바와 같이 아텔로콜라겐 함량은 실시예 1과 비교예 3(0.01 중량%의 아텔로콜라겐)에서 유사하게 검출되었으므로, 실시예 1의 제조 시 아텔로콜라겐은 안정적으로 유지됨을 확인할 수 있었다.As a result of SDS-PAGE, as shown in Fig. 3, the atelocollagen content was similarly detected in Example 1 and Comparative Example 3 (0.01 wt% of atelocollagen), so that in the preparation of Example 1, It can be confirmed that it is maintained stably.

시험예Test Example 2. 피부 보습 효과 측정 2. Skin moisturizing effect measurement

공기의 이동과 직사광선이 없으며, 항온항습조건(22 ±2℃, 50 ±5%)이 유지되는 공간에서 한국인 여성 20 명을 최소 15 분 이상 대기시킨 후, 실시예 1의 화장료 조성물을 얼굴 피부에 도포하여 수분량을 측정하였다. 수분량은 코니오미터(corneometer, Courage+Khazaka GmbH, 독일)를 사용하여 도포 개시 전, 도포 30 분 후, 도포 3 주 후에 각각 측정하였다.20 Korean women were allowed to stand for at least 15 minutes in a space free from air movement and direct sunlight and maintained in a constant temperature and humidity condition (22 ± 2 ° C, 50 ± 5%), and then the cosmetic composition of Example 1 was applied to face skin And the water content was measured. Moisture content was measured before the start of application, 30 minutes after application, and 3 weeks after application using a corneometer (Courage + Khazaka GmbH, Germany).

그 결과, 도 4에 표시된 바와 같이 실시예 1의 조성물 도포 후 얼굴 피부의 수분량은 도포 전과 비교하여 유의하게 증가한 것으로 나타났다(p<0.05).As a result, as shown in FIG. 4, the water content of the facial skin after the application of the composition of Example 1 was significantly increased (p <0.05) as compared with that before application.

시험예Test Example 3. 피부 각질 개선 효과 측정 3. Measuring the effect of improving skin's keratin

공기의 이동과 직사광선이 없으며, 항온항습조건(22 ±2℃, 50 ±5%)이 유지되는 공간에서 한국인 여성 20 명을 최소 15 분 이상 대기시킨 후, 실시예 1의 화장료 조성물을 얼굴 피부에 도포하여 각질량을 측정하였다. 각질량은 Black D-squame® (Cuderm Co., 미국)을 사용하여 도포 개시 전과 도포 3 주 후에 각각 측정하였다.20 Korean women were allowed to stand for at least 15 minutes in a space free from air movement and direct sunlight and maintained in a constant temperature and humidity condition (22 ± 2 ° C, 50 ± 5%), and then the cosmetic composition of Example 1 was applied to face skin And each mass was measured. Each weight was measured respectively by using the Black D-squame ® (Cuderm Co. , USA) applying the coating before and three weeks after initiation.

그 결과, 도 5에 표시된 바와 같이, 실시예 1의 조성물 도포 후 얼굴 피부의 각질량은 도포 전과 비교하여 유의하게 감소한 것으로 나타났다(p<0.05).As a result, as shown in Fig. 5, the masses of the facial skin after application of the composition of Example 1 were significantly decreased (p < 0.05) compared with those before application.

시험예Test Example 4.  4. 사용감Feeling 만족도 설문조사 Satisfaction Survey

한국인 여성 20명을 대상으로 실시예 1의 화장료 조성물을 얼굴 피부에 도포하여, 3 주 후 보습 및 각질 개선 효과에 대한 설문조사를 실시하였다. 또한 화장품으로써의 최종 만족도를 평가하였다.Twenty Korean women applied the cosmetic composition of Example 1 to the facial skin and conducted a questionnaire survey on the effect of moisturizing and exfoliation improvement after 3 weeks. We also evaluated the final satisfaction as a cosmetic product.

실시예 1에 대한 보습 및 각질 개선 효과 설문 평가 결과 Evaluation results of moisturizing and exfoliation improvement effect for Example 1

구분


division

보습 효과

Moisturizing effect

각질 개선 효과

Effect of exfoliation

피험자 수(명)

Number of subjects (persons)

백분율(%)

percentage(%)

피험자 수(명)

Number of subjects (persons)

백분율(%)

percentage(%)

매우 좋다

very good

8

8

40

40

3

3

15

15

좋다

good

12

12

60

60

13

13

65

65

조금 좋다

A little good

0

0

0

0

2

2

10

10

효과 없음

no effect

0

0

0

0

2

2

10

10

긍정적 답변의 합
(매우 좋다~조금좋다)

Sum of positive answers
(Very good ~ a little good)

20

20

100

100

18

18

90

90

실시예 1에 대한 전체적인 만족도 설문 평가 결과The overall satisfaction survey result for Example 1
구분

division

전체적인 시료 만족도

Overall sample satisfaction

피험자 수(명)

Number of subjects (persons)

백분율(%)

percentage(%)

매우 좋다

very good

4

4

20

20

좋다

good

14

14

70

70

조금 좋다

A little good

2

2

10

10

보통이다

is average

0

0

0

0

좋지 않다

Not good

0

0

0

0

긍정적 답변의 합
(매우 좋다~조금좋다)

Sum of positive answers
(Very good ~ a little good)

20

20

100

100

상기 표 2, 3에 표시된 바와 같이, 보습 효과에 대한 긍정적인 답변 20 명 (100%), 각질 개선 효과에 대한 긍정적 답변 18 명(90%), 전체적인 만족도에 대한 긍정적인 답변이 20 명(100%)으로 조사되었다.As shown in Tables 2 and 3, 20 (100%) positive responses to the moisturizing effect, 18 (90%) positive responses to the exfoliation improvement effect, and 20 %).

Claims (5)

아텔로콜라겐을 함유하는 화장료 조성물로써, 투명 피부 보호막을 형성하는 것을 특징으로 하는 화장료 조성물
A cosmetic composition containing atelocollagen, which is characterized by forming a transparent skin protective film
제 1 항에 있어서, 상기 아텔로콜라겐은 화장료 조성물 총 중량에 대하여 0.01 내지 1.0 중량%로 함유되는 것을 특징으로 하는 화장료 조성물
The cosmetic composition according to claim 1, wherein the atelocollagen is contained in an amount of 0.01 to 1.0% by weight based on the total weight of the cosmetic composition
제 1 항에 있어서, 상기 조성물은 피부 보습용 또는 피부 각질 개선용임을 특징을 하는 화장료 조성물
The cosmetic composition according to claim 1, wherein the composition is used for moisturizing the skin or for improving the horny skin
제 1항에 있어서, 상기 조성물은 화장수(스킨로션) 제형임을 특징으로 하는 화장료 조성물
The cosmetic composition according to claim 1, wherein the composition is a cosmetic lotion (skin lotion) formulation
제 1 항에 있어서, 상기 조성물은 정제수, 부틸렌글라이콜, 글리세린, 1,2-헥산디올, 아텔로콜라겐, 셀룰로오스검, 소듐 하이알루로네이트, 소듐락테이트, 락틱애씨드를 포함하는 것을 특징을 하는 화장료 조성물The composition according to claim 1, wherein the composition comprises purified water, butylene glycol, glycerin, 1,2-hexanediol, atelocollagen, cellulose gum, sodium hyaluronate, sodium lactate, lactic acid, Cosmetic composition
KR1020160068385A 2016-06-01 2016-06-01 Cosmetic compositions of the formulation forming transparent skin protective film KR20170136375A (en)

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JPS62266064A (en) * 1986-05-14 1987-11-18 株式会社 高研 Production of collagen membrane
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