KR20170113377A - Method for producing nanoemulsion containing cultivated wild ginseng fermentate and cosmetic composition for skin whitening comprising the nanoemulsion - Google Patents

Abstract

The present invention relates to a method for producing a nanoemulsion comprising a fermented product of a wild ginseng fermented milk and a cosmetic composition for skin whitening comprising the fermented product of the fermented product of the wild ginseng cultured muscle. The nano emulsion has a high rate of percutaneous absorption, The fermented product of the wild ginseng cultured muscle is excellent in improving skin whitening, and thus can be usefully used in cosmetic compositions for skin whitening.

Description

TECHNICAL FIELD The present invention relates to a method for producing a nanoemulsion comprising a fermented product of a wild ginseng cultured muscle and a cosmetic composition for skin whitening comprising the fermented product of a wild ginseng cultured muscle,

The present invention relates to a method for producing a nanoemulsion comprising a fermented product of a wild ginseng cultured muscle and a cosmetic composition for skin whitening comprising the same.

Panax ginseng CA Mayer (1843) has the meaning of 'panacea rule'. Wild ginseng is a perennial rootstock belonging to the Araliaceae. It resembles the shape of a person, and is a medicinal plant with roots. Wild ginseng is classified into four kinds of ginseng, ginseng, race and ginseng. Ten kinds, root species, and race are wild birds, which means that birds eat seeds and then excrete them in the mountains. Camphor ginseng is an artificially cultivated wild ginseng seed in the mountains.

The ginseng cultivating muscle is an adventitious roots formed by totipotency of a plant by removing part of the tissue of wild ginseng and introducing it aseptically into the cabin. The ginseng cultivated muscle can be mass-produced by culturing the ginseng adventitious roots for 20 ~ 30 days in pre-culture (Seed culture) and then in the bioreactor for 40 ~ 120 days. The remaining pesticides (Diazinon, DDT, Metalaxyl) , Cd), and so on, so that safe wild ginseng can be obtained from pests and viral infections that may occur during the natural growth.

Saponins and polyphenol compounds (Rb1, Rb2, Rh1, Rg1, Rg2) in saponin containing almost no ginsenosides such as ginsenosides Rg5, Rk1, Rh2 and Rg3 Polyphenolic compound, polyacetylene, alkaloid, and minerals (Ge, Se, Mn, V, Ti, etc.).

The nano emulsion has a size of about 50 to 200 nm in diameter and has a translucent appearance because of its small particle size. Unlike microemulsions, nanoemulsions are free from agglomeration and coalescence between particles and free from sedimentation or creaming due to their unaffected gravity, so they can be physically stable for a long time. In addition, since the content of surfactant, which is industrially important, is small, it can be applied to various fields such as pharmaceuticals, cosmetics and agriculture. In general, nano emulsions are prepared by applying mechanical energy to the system using a shear mixer or by using high energy such as a high-pressure emulsifier or an ultrasonic wave.

Korean Patent No. 10-1315440 discloses a nanoemulsion containing a wild ginseng extract and a personal care composition comprising the same, and Korean Patent No. 10-1117565 discloses a cosmetic composition for improving skin wrinkles containing a fermentation broth of cinnamon ginseng However, the method for producing the nanoemulsion including the fermented product of the wild ginseng cultured muscle of the present invention and the skin whitening cosmetic composition containing the fermented product have not yet been disclosed.

Bacillus circulans , Leuconostoc mesenteroides , and Bacillus subtilis are preferred as fermentation strains of wild ginseng cultured muscle , In contrast to the strains, Pediococcus pentosaceus strain of the present invention showed the highest inhibitory activity against tyrosinase and that the nano emulsion prepared at a specific blending ratio containing the same showed excellent emulsification state and stability, Completed.

In order to achieve the above object, the present invention provides a process for preparing a mixture comprising: (a) mixing and stirring 1.0 to 10% by weight of hydrogenconed lecithin and 67.5 to 69% by weight of water; (b) adding 10 to 30% by weight of Dipotassium Glycyrrhizate to the mixture prepared in step (a), and stirring the mixture in a hot plate at 60 to 100 ° C to prepare a mixture; (c) after step (b), 0.3 to 0.7 wt% of PEG-60 Hydrogenated Castor Oil, 0.3 to 0.7 wt% of cholesterol, and 3 to 15 wt% of car Preparing a mixture by mixing and stirring the caprylic / capric triglyceride in a hot plate at 60 to 100 ° C; (d) mixing the mixture prepared in the step (c) and the fermented product of 0.5 to 1.5% by weight of the ginseng cultured muscle at 60 to 100 ° C. and 1,000 to 3,000 rpm for 5 to 20 minutes, Adding to the mixture and emulsifying to prepare an emulsified mixture; (e) cooling the emulsion mixture prepared in step (d) to 10 to 25 DEG C; And (f) repeatedly passing the emulsified mixture cooled in the step (e) through a high-pressure homogenizer at 900 to 1,100 bar for 8 to 10 times to convert into a nano-emulsion. And a method for producing the nano emulsion.

The present invention also relates to a fermented product of a wild ginseng cultured muscle in an amount of 0.5 to 1.5% by weight; 1 to 10% by weight of hydrogenated lecithin; 0.3 to 0.7% by weight of a Pigment-60 hydrogeneated castor oil; 10 to 30% by weight of dipotassium glycyrrhizate; 0.3 to 0.7% by weight of cholesterol; 3-15% by weight caprylic / capric triglyceride; And 67.5 to 69 wt% water.

The present invention also provides a cosmetic composition for skin whitening comprising a nanoemulsion containing the fermented product of the wild ginseng cultured muscle.

The present invention relates to a method for producing a nanoemulsion comprising a fermented product of a wild ginseng fermented milk and a cosmetic composition for skin whitening comprising the fermented product of the fermented product of the wild ginseng cultured muscle. The nano emulsion has a high rate of percutaneous absorption, The fermented product of the wild ginseng cultured muscle is excellent in improving skin whitening, and thus can be usefully used in cosmetic compositions for skin whitening.

1 is a graph showing particle distributions of a nano emulsion and a general emulsion containing a fermented product of a wild ginseng culture muscle of the present invention.

In order to accomplish the object of the present invention, the present invention provides a method for preparing a mixture comprising: (a) mixing and stirring 1.0 to 10 wt% of hydrogenated lecithin and 67.5 to 69 wt% of water to prepare a mixture;

(b) adding 10 to 30% by weight of Dipotassium Glycyrrhizate to the mixture prepared in step (a), and stirring the mixture in a hot plate at 60 to 100 ° C to prepare a mixture;

(c) after step (b), 0.3 to 0.7 wt% of PEG-60 Hydrogenated Castor Oil, 0.3 to 0.7 wt% of cholesterol, and 3 to 15 wt% of car Preparing a mixture by mixing and stirring the caprylic / capric triglyceride in a hot plate at 60 to 100 ° C;

(d) mixing the mixture prepared in the step (c) and the fermented product of 0.5 to 1.5% by weight of the ginseng cultured muscle at 60 to 100 ° C. and 1,000 to 3,000 rpm for 5 to 20 minutes, Adding to the mixture and emulsifying to prepare an emulsified mixture;

(e) cooling the emulsion mixture prepared in step (d) to 10 to 25 DEG C; And

(f) repeatedly passing the emulsified mixture cooled in step (e) through a high pressure homogenizer at 900 to 1,100 bar for 8 to 10 times to convert it into a nanoemulsion. The present invention provides a method for producing a nano emulsion.

In the method for producing the nanoemulsion containing the fermented product of the wild ginseng cultured muscle of the present invention,

(a) mixing and stirring 3% by weight of hydrogenconed lecithin and 68% by weight of water to prepare a mixture;

(b) stirring the mixture prepared in step (a) in a hot plate at 80 DEG C while adding 20 wt% of dipotassium glycyrrhizate to prepare a mixture;

(c) after step (b), 0.5% by weight of a Pigi-60 hydrogenated castor oil, 0.5% by weight of cholesterol and 7% by weight of caprylic / capric triglyceride are mixed And stirring to prepare a mixture;

(d) adding the mixture prepared in the step (c) and the fermented product of 1% by weight of the wild ginseng cultured muscle to the mixture prepared in the step (b) under stirring at 80 DEG C and 2,000 rpm for 15 minutes, Lt; / RTI >

(e) cooling the emulsion mixture prepared in step (d) to 20 캜; And

(f) repeatedly passing the emulsified mixture cooled in the step (e) through a high-pressure homogenizer at 1000 bar for 10 times to convert it into a nano-emulsion.

The present invention also relates to a fermented product of a wild ginseng cultured muscle in an amount of 0.5 to 1.5% by weight; 1 to 10% by weight of hydrogenated lecithin; 0.3 to 0.7% by weight of a Pigment-60 hydrogeneated castor oil; 10 to 30% by weight of dipotassium glycyrrhizate; 0.3 to 0.7% by weight of cholesterol; 3-15% by weight caprylic / capric triglyceride; And 67.5 to 69 wt% water.

In the nanoemulsion of the present invention, the hydrogenated lecithin may be contained in the nano emulsion in an amount of 1 to 10% by weight, preferably 3% by weight, but is not limited thereto. If it is contained in an amount of less than 1% by weight, the stability of emulsification may be deteriorated, and if it exceeds 10% by weight, the viscosity may become too high.

In addition, the paper-60 hydrogenated castor oil may contain 0.3 to 0.7% by weight, preferably 0.5% by weight, of the nanoemulsion, but is not limited thereto. If it is contained in an amount of less than 0.3% by weight, there may be a problem in formation of a nano emulsion. If it is contained in an amount exceeding 0.7% by weight, the amount of a component encapsulated in the emulsion is reduced due to excessive membrane components, May be insufficient.

The preparation of the nanoemulsion can be carried out by various methods known in the art and there is no particular limitation thereon.

The diameter of the nanoemulsion may be 50 to 300 nm, preferably 100 to 200 nm, but is not limited thereto.

In addition, the fermented product of the wild ginseng cultured muscle may be fermented using a strain of Pseudococcus pentosaceus , but the present invention is not limited thereto.

In addition, the fermented product of the wild ginseng cultured muscle may promote skin whitening, but is not limited thereto.

In addition, the fermented product of the wild ginseng cultured muscle

(a) tissue culture of wild ginseng adrenal glands;

(b) subjecting the tissue culture product of step (a) to high temperature and high pressure treatment followed by hot air drying;

(c) concentrating the hot-air dried product of step (b) under reduced pressure;

(d) suspending the reduced pressure concentrate of step (c) in water;

(e) fermenting the suspension of step (d) with a strain of Pediococcus pentosaceus ;

(f) heat treating the fermentation product of step (e); And

(g) concentrating the heat-treated product of step (f), but the present invention is not limited thereto.

In addition, the fermented product of the wild ginseng cultured muscle may contain 0.5-1.5 wt% of the nanoemulsion, preferably 1 wt%, but is not limited thereto.

The nanoemulsion is preferably

(a) mixing and stirring 1.0 to 10% by weight of hydrogenated lecithin and 67.5 to 69% by weight of water to prepare a mixture;

(b) adding 10 to 30% by weight of Dipotassium Glycyrrhizate to the mixture prepared in the step (a), stirring the mixture in a hot plate at 60 to 100 ° C to prepare a mixture;

(c) after step (b), 0.3 to 0.7 wt% of PEG-60 Hydrogenated Castor Oil, 0.3 to 0.7 wt% of cholesterol, and 3 to 15 wt% of car Preparing a mixture by mixing and stirring the caprylic / capric triglyceride in a hot plate at 60 to 100 ° C;

(d) the mixture prepared in step (c); And wild ginseng cultured muscle were cultured in Pediococcus pentosaceus fermented with 0.5 to 1.5 wt% of ginseng fermented milk was added to the mixture prepared in step (b) while stirring at 60 to 100 ° C and 1,000 to 3,000 rpm for 5 to 20 minutes, To produce an emulsified mixture;

(e) cooling the emulsion mixture prepared in step (d) to 10 to 25 DEG C; And

(f) passing the emulsified mixture cooled in the step (e) through a high-pressure homogenizer at 900 to 1,100 bar repeatedly for 8 to 10 times to convert it into a nano-emulsion. It is not limited.

The present invention also provides a cosmetic composition for skin whitening comprising the nanoemulsion.

In the skin whitening cosmetic composition of the present invention, the nano emulsion may be contained in an amount of 1 to 20% by weight, preferably 10% by weight, but is not limited thereto.

On the other hand, the whitening effect refers to an effect to improve or prevent glaring skin, spots, freckles, and dark circles caused by various factors such as exposure to ultraviolet rays, change in hormone balance, genetic program, Or an effect of maintaining a beautiful skin with a sense of transparency and an effect of reducing the dullness of the skin to increase gloss and tautness. In general, it is known that the skin, spots, freckles, and dark circles of the skin, stamens, freckles and dark circles are stimulated by melanocytes due to stimulation by ultraviolet rays or changes in hormone balance, and melanin pigment is deposited on the skin. Therefore, if the production of melanin can be inhibited, it is possible to prevent or improve gross skin, spots, freckles and dark circles.

In the cosmetic composition according to one embodiment of the present invention, the cosmetic composition for skin whitening can be used as an external skin ointment, a cream, a softening longevity, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing Lotions, cleansing creams, body lotions, and body cleansers. However, the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, at least one skin whitening active ingredient exhibiting the same or similar functions. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenylalkanol, 2,7-dinitroindazole or Plant extracts such as mandarin orange extract, rice extract, licorice extract, and the like, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is an interfacial-active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example  1. By strain Wild ginseng cultivator Fermented  Identify manufacturing and whitening activity

Panax ginseng CAMeyer. The wild ginseng collected from Yanggu - gu, Gangwon - do, was introduced into the cabin and cultured. The cultured wild ginseng cultured muscle was incubated for 7 weeks with high temperature and high pressure, and then dried by hot air to adjust the water content to below 5% and concentrated under reduced pressure to prepare 60 Brix concentrate. To the concentrate, 12 times (w / w) of purified water was added to make a suspension, followed by inoculation with a strain of Pediococcus pentosaceus , followed by fermentation for 1 day. After completion of the fermentation, the fermentation broth was treated at 80 DEG C for 30 minutes to inactivate the inoculated strain. Subsequently, the fermentation broth was concentrated under reduced pressure to prepare a 60 Brix concentrate. In the present invention, Pediococcus pentosaceus ) as a comparative strain, Bacillus circulans , Leuconostoc mesenteroides and Bacillus subtilis were used to prepare a fermented concentrate.

The tyrosinase inhibitory activity of the strain-based fermentation concentrate (hereinafter referred to as "sample") was determined according to the method of Ishihara, Y. et al., 1991, J. Antibiot., 44, 50 μl of a phosphate buffer solution of M, 50 μl of a sample and 50 μl of a mushroom tyrosinase (150-200 U / ml) were added in this order and then 50 μl of a 1.5 mM tyrosine solution was added thereto. The mixture was reacted at 37 ° C. for 10 minutes, Were measured. As a result, three to five Phedi Lactococcus pen soil of the present invention as disclosed in Table 1 mouse (Pediococcus pentosaceus strains were significantly superior to tyrosinase inhibitors in the wild ginseng culture fermentations prepared by using different strain strains. Therefore, the fermented product of the wild ginseng cultured muscle prepared by inoculating a strain of Pediococcus pentosaceus was used for producing the nanoemulsion of the present invention.

Tyrosinase inhibitory activity of fermented products of cultured ginseng cultured on fermentation strain The fermented product of the wild ginseng cultured muscle (100 / / ml) Pedio Caucus
Pentosaceus Bacillus
Circularans Lucono Stock
Messeneroides Bacillus
Servillis Tyrosinase
Inhibitory activity (%) 73.2 ± 0.9 40.1 ± 1.9 47.1 ± 1.2 39.0 ± 0.2

Example  2. Nano-emulsion  Determination of the optimum compounding ratio and evaluation of its stability

The fermented product of the wild ginseng culture broth and each ingredient were dissolved at a temperature of 80 ° C in the composition shown in Table 2 (mixing ratio 1 to 4), and then mixed at 2,000 rpm for 15 minutes. Then, the mixture was continuously fed at 1,000 bar in a high pressure homogenizer After passing 10 times, the mixture was cooled and defoamed to prepare each of the nanoemulsions having a compounding ratio of 1 to 4.

Preparation of nano emulsion according to mixing ratio Component (% by weight) Compounding ratio 1 Compounding ratio 2 Compounding ratio 3 Compounding ratio 4 Fermented product of wild ginseng cultured muscle One 2 One 2 Hydrogenated lecithin 3 3 3 3 Dipotassium glycyrrhizate 20 20 20 20 PIGGY-60 Hydrogenated Castor Oil One One 0.5 0.5 Cholesterol USP - - 0.5 0.5 Tegosoft CT 7 7 7 7 Distilled water 68 67 68 67 Sum 100 100 100 100

The method for producing the nanoemulsion (compounding ratio 3) of the present invention is as follows. Specifically, to the water beaker, about 1,000 g of distilled water was mixed with 45 g of the hydrogencontained lecithin to allow the hydrogenated lecithin to sufficiently absorb water, 300 g of dipotassium glycyrrhizate was further added, The mixture was heated on a hot plate and heated to 80 DEG C while stirring. Thereafter, 7.5 g of the phage-60 hydrogenated castor oil, 7.5 g of cholesterol USP and 105 g of Tegosoft CT (Caprylic / Capric Triglyceride) were added to the oil-based beaker, and heated to 80 ° C Lt; / RTI > After confirming that the mixture of the water beaker and the oil beaker were all melted at 80 ° C, a mixture of the oil-free beaker and 15.0 g of the fermented product of the ginseng fermenter was stirred at 80 ° C while stirring the mixture of the water beaker at 2,000 rpm. And then slowly added to the mixture of the water beaker sequentially to prepare an emulsified mixture. The resulting emulsified mixture was slowly cooled to 20 ° C with stirring, and then passed through a high-pressure homogenizer at 1000 bar for 10 times to prepare a nano-emulsion containing fermented ginseng root of about 185 nm in diameter One). Also, a method of producing a nano emulsion composed of blending ratios 1, 2 and 4 was applied in the same manner as the above method. The emulsion state after the preparation of the nanoemulsion having the compounding ratio of 1 to 4 was good except for the compounding ratio of 2, but the temporary stability was evaluated at 40 占 폚 for one month. As a result, it was confirmed that the oil phase was separated at some mixing ratio there was. In the case of mixing ratios 3 and 4, the nano emulsion prepared at a compounding ratio of 3 was more excellent in emulsion state and stability and was selected as the final mixing ratio (Table 3).

Identification of emulsion state and temporary stability immediately after preparation of nano emulsion according to mixing ratio ingredient Compounding ratio 1 Compounding ratio 2 Compounding ratio 3 Compounding ratio 4 Emulsion state immediately after manufacture ○ △ ◎ ○ Temporary stability
(1 month at 40 < 0 > C) △ △ ◎ ○

?: Medium,?: Good,?: Very good

Comparative Example  One. Wild ginseng cultivator The fermented product  General containing emulsion  Produce

In Example 2, an emulsion containing a fermented product of ginseng cultured muscle having a diameter of about 18,280 nm was prepared by repeating 0 times repeatedly under the condition of 1,000 bar using a high-pressure homogenizer (FIG. 1).

Comparative Example  2. Wild ginseng cultivator The fermented product  Containing emulsion  Produce

In Example 2, an emulsion containing a fermented product of a ginseng fermented milk having a diameter of about 418 nm was prepared by repeating six times repeatedly at 1,000 bar using a high-pressure homogenizer (FIG. 1).

Example  3. Wild ginseng cultivator The fermented product  Containing Nano emulsion  Manufacture of essences containing

Essences containing nanoemulsions containing the fermented products of the wild-type ginseng root fermentation product of Example 2 were prepared by the conventional method with the composition shown in Table 4 below.

Content of nanoemulsion essence containing fermented product of wild ginseng cultured muscle (%) ingredient Example 3 (% by weight) ingredient Comparative Example 3 (% by weight) Fermented wild ginseng culture nanoemulsion
(Example 2) 10 Fermented wild ginseng cultured muscle general emulsion
(Comparative Example 1) 10 Carbomer One Carbomer One Butylene glycol 5 Butylene glycol 5 Hyaluronic acid 15 Hyaluronic acid 15 Triethanolamine One Triethanolamine One Hexanediol caprylic < RTI ID = 0.0 > glycol 0.5 Hexanediol caprylic < RTI ID = 0.0 > glycol 0.5 Purified water 67.5 Purified water 67.5 Sum 100 Sum 100

Comparative Example  3. Wild ginseng cultivator The fermented product  Containing General emulsion  Manufacture of essences containing

The essence was prepared in the same manner as in Example 2 except that a normal emulsion containing the fermented product of the wild-type ginseng root of Comparative Example 1 was added to the composition of Table 4 in place of the nanoemulsion containing the fermented product of the wild ginseng fermented product of Example 2 above.

Experimental Example  One. Wild ginseng cultivator The fermented product  Containing Nano-emulsion  Particle distribution measurement

The particle distributions of the nanoemulsion prepared in Example 1 and Comparative Examples 1 and 2 were measured using a particle size analyzer (Particle Size Analyzer, Malvern Ins. Ltd., model matersizer 2000). As a result, Had an average particle diameter size of 185 nm, whereas Comparative Example 1 had an average particle diameter of about 18,280 nm, and Comparative Example 2 had an average particle diameter of 418 nm, indicating that the size of the nanoemulsion of the present invention was much smaller I could.

Experimental Example  2. Constellation stability test

The property stability of the essence prepared in Example 3 and Comparative Example 3 was tested. The essences prepared in Example 3 and Comparative Example 3 were stored in an opaque glass container in a thermostat kept constant at 45 ° C and stored for 12 weeks. The essence prepared in Example 3 and Comparative Example 3 was stored at 4 ° C Lt; RTI ID = 0.0 > 12 < / RTI > weeks in an opaque glazed container. In addition, the essences prepared in Example 3 and Comparative Example 3 were stored for 12 weeks in an opaque box with temperature cycling (low temperature of 4 캜 and high temperature of 45 캜). Then, the degree of separation and degree of discoloration of the essence prepared in Example 3 and Comparative Example 3 were compared and the degree of separation and discoloration was classified into the following six grades and evaluated.

 0: No change; 1: very little separation (discoloration); 2: little separation (discoloration); 3: Separated slightly (discoloration); 4: Severely separated (discolored); And 5: Severely separated (discolored)

In addition, the degree of precipitation of the essence prepared in Example 3 and Comparative Example 3 was compared, and the degree of precipitation was classified into the following six grades and evaluated.

0: no change, 1: very little precipitation, 2: little precipitation, 3: slightly heavily precipitated, 4: heavily precipitated, 5:

As a result, as shown in the following Table 5, the essence of Example 3 was stable without discoloration or separation phenomenon at 4 ° C, 45 ° C and temperature circulation (4 ° C and 45 ° C) And it was confirmed that there was severe separation and discoloration. In addition, although the essence of Example 3 did not cause precipitation, it was confirmed that the essence of Comparative Example 3 caused precipitation (Table 6).

The separation and discoloration degree of the essence prepared in Example 3 and Comparative Example 3 Temperature Degree of separation and discoloration Example 3 Comparative Example 3 45 ° C 0 3 4 ℃ 0 2 Circulation (4 ° C and 45 ° C) 0 3

The degree of precipitation of the essence prepared in Example 3 and Comparative Example 3 Temperature Degree of precipitation Example 3 Comparative Example 3 45 ° C 0 3 4 ℃ 0 2 Circulation (4 ° C and 45 ° C) 0 3

Experimental Example  3. Invitro  Tyrosinase ( in vitro tyrosinase ) Active inhibition test

Tyrosinase is an enzyme involved in determining the most important initial rate in the melanin biosynthetic pathway in the human body. Since many whitening ingredients have an action mechanism that inhibits this enzyme, this test inhibits the activity of tyrosinase in vitro And the degree to which it is evaluated. The tyrosinase inhibitory activity of the wild type ginseng root extract, ginseng root fermented product, ginseng extract, ginseng fermented product and red ginseng fermented product at the same concentration (1%) was measured. As shown in Table 7, The inhibitory activity of tyrosinase was about 3 to 9 times higher than that of ginseng extract, ginseng fermented product or red ginseng fermented product.

Inhibitory activity of tyrosinase of ginseng root and ginseng and red ginseng Sample Treatment concentration (%) Tyrosinase
Inhibitory activity (%) Ginseng cultured root extract One 35 Fermented product of wild ginseng cultured muscle One 45 Ginseng extract One 5 Ginseng fermentation product One 7 Fermented red ginseng One 12

Claims (8)

(a) mixing and stirring 1.0 to 10% by weight of hydrogenated lecithin and 67.5 to 69% by weight of water to prepare a mixture;
(b) adding 10 to 30% by weight of Dipotassium Glycyrrhizate to the mixture prepared in the step (a), stirring the mixture in a hot plate at 60 to 100 ° C to prepare a mixture;
(c) after step (b), 0.3 to 0.7 wt% of PEG-60 Hydrogenated Castor Oil, 0.3 to 0.7 wt% of cholesterol, and 3 to 15 wt% of car Preparing a mixture by mixing and stirring the caprylic / capric triglyceride in a hot plate at 60 to 100 ° C;
(d) mixing the mixture prepared in the step (c) and the fermented product of 0.5 to 1.5% by weight of the ginseng cultured muscle at 60 to 100 ° C. and 1,000 to 3,000 rpm for 5 to 20 minutes, Adding to the mixture and emulsifying to prepare an emulsified mixture;
(e) cooling the emulsion mixture prepared in step (d) to 10 to 25 DEG C; And
(f) repeatedly passing the emulsified mixture cooled in step (e) through a high pressure homogenizer at 900 to 1,100 bar for 8 to 10 times to convert it into a nanoemulsion. Gt; 0.5 to 1.5% by weight fermented product of cultured wild ginseng; 1 to 10% by weight of hydrogenated lecithin; 0.3 to 0.7% by weight of a Pigment-60 hydrogeneated castor oil; 10 to 30% by weight of dipotassium glycyrrhizate; 0.3 to 0.7% by weight of cholesterol; 3-15% by weight caprylic / capric triglyceride; And 67.5 to 69% by weight of water. The nanoemulsion according to claim 2, wherein the diameter of the nanoemulsion is 50 to 300 nm. [3] The fermented product according to claim 2, wherein the fermented product of the wild ginseng cultured muscle is selected from the group consisting of Pediococcus pentosaceus ). < / RTI > [Claim 3] The nanoemulsion according to claim 2, wherein the fermented product of the wild ginseng cultured muscle promotes skin whitening. [Claim 3] The fermented product according to claim 2,
(a) tissue culture of wild ginseng adrenal glands;
(b) subjecting the tissue culture product of step (a) to high temperature and high pressure treatment followed by hot air drying;
(c) concentrating the hot-air dried product of step (b) under reduced pressure;
(d) suspending the reduced pressure concentrate of step (c) in water;
(e) fermenting the suspension of step (d) with a strain of Pediococcus pentosaceus ;
(f) heat treating the fermentation product of step (e); And
(g) concentrating the heat-treated product of step (f). 3. The composition of claim 2, wherein the nanoemulsion comprises
(a) mixing and stirring 1.0 to 10% by weight of hydrogenated lecithin and 67.5 to 69% by weight of water to prepare a mixture;
(b) adding 10 to 30% by weight of Dipotassium Glycyrrhizate to the mixture prepared in the step (a), stirring the mixture in a hot plate at 60 to 100 ° C to prepare a mixture;
(c) after step (b), 0.3 to 0.7 wt% of PEG-60 Hydrogenated Castor Oil, 0.3 to 0.7 wt% of cholesterol, and 3 to 15 wt% of car Preparing a mixture by mixing and stirring the caprylic / capric triglyceride in a hot plate at 60 to 100 ° C;
(d) the mixture prepared in step (c); And wild ginseng cultured muscle were cultured in Pediococcus pentosaceus fermented with 0.5 to 1.5 wt% of ginseng fermented milk was added to the mixture prepared in step (b) while stirring at 60 to 100 ° C and 1,000 to 3,000 rpm for 5 to 20 minutes, To produce an emulsified mixture;
(e) cooling the emulsion mixture prepared in step (d) to 10 to 25 DEG C; And
(f) repeatedly passing the emulsified mixture cooled in the step (e) through a high-pressure homogenizer at a temperature of 900 to 1,100 bar for 8 to 10 times to convert it to a nano-emulsion. Nano emulsion. A cosmetic composition for skin whitening comprising the nanoemulsion of any one of claims 2 to 7.

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