KR20170028383A - Rna 전사물 변이체의 정량분석을 위한 방법 및 생성물 - Google Patents

Rna 전사물 변이체의 정량분석을 위한 방법 및 생성물 Download PDF

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KR20170028383A
KR20170028383A KR1020177002619A KR20177002619A KR20170028383A KR 20170028383 A KR20170028383 A KR 20170028383A KR 1020177002619 A KR1020177002619 A KR 1020177002619A KR 20177002619 A KR20177002619 A KR 20177002619A KR 20170028383 A KR20170028383 A KR 20170028383A
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루카스 파울
페트라 쿠발라
토르스텐 레다
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Abstract

본 발명은 전사체학의 분야에 관한 것이며, 전사물 변이체를 모방하는 인공 폴리핵산 분자의 참조 세트를 제공하고 상기 참조 세트를 외부 대조용으로서 전사물 변이체를 포함하는 샘플에 가함을 포함하는, 샘플 중 전사물 변이체의 조절된 식별 및/또는 정량분석 방법을 제공한다. 본 발명은 또한 상기와 같은 참조 세트뿐만 아니라 상기와 같은 참조 세트의 제조 방법을 제공한다.

Description

RNA 전사물 변이체의 정량분석을 위한 방법 및 생성물{METHODS AND PRODUCTS FOR QUANTIFYING RNA TRANSCRIPT VARIANTS}
본 발명은 전사체학, 특히 전체 전사체 샷건 서열분석("RNA-seq") 분야에 관한 것이다. 보다 구체적으로, 본 발명은 RNA-seq 또는 마이크로-어레이 분석 또는 정량적인 PCR(qPCR)에 의해 분석된 샘플 중 RNA 전사물 변이체의 식별 및 정량분석에 적합한 방법 및 생성물에 관한 것이다.
차세대 서열분석(NGS) 기술은 핵산 샘플의 서열분석시 다량의 짧은 리드(read)를 생성시킨다. 차세대 서열분석의 필수 단계는 라이브러리 제조 또는 간략히 라이브러리 프렙(prep)이다. 상기 과정은 mRNA 또는 cDNA를 투입으로서 취하여, 각각 mRNA 분자의 섹션에 상응하는 짧은 cDNA 단편들의 라이브러리를 생성시킨다. 이어서 상기 단편을 NGS 서열분석기에 의해, 대개는 상기 단편 전체가 아니라 부분적으로 그의 개시 및/또는 단부에서 서열분석한다. 그 결과 리드라 칭하는 뉴클레오티드의 짧은 서열들이 생성되며 이들은 가장 통상적으로는 4개의 ASCII 문자, 예를 들어 A, C, G, T 또는 0, 1, 2, 3(유전 암호의 핵염기를 나타낸다) 그룹의 서열로서 상기 NGS 서열분석기에 의해 저장된다. mRNA 분자가 원래의 샘플 중에 존재하였음을 추단하기 위해서, 상기 리드를 참조 게놈(reference genome) 또는 전사체상에 지도화(mapping)하거나 또는 정렬시키거나, 또는 서열 오버랩을 근거로 드노보 조립한다.
차세대 서열분석은 다양한 게놈 지도화 과정(US 2013/110410 A1) 또는 DNA 식별 방법에, 예를 들어 지도화된 게놈을 사용하여 서열 리드를 몇몇 유기체 변이체에 관련시킴으로써(WO 2009/085412 A1) 사용되어 왔다.
WO 2009/091798 A1은 유기체 전사체의 프로파일을 획득하는 방법을 기재하며, 상기 방법은 하나 이상의 cDNA 분자를 서열분석하여 서열분석 리드들을 획득하고; 각각의 상기 서열분석 리드를 참조 서열에 대해 정렬시킴을 포함한다.
그러나, 짧은 서열 리드를 사용하는 전사체 분석에 근원하는 주요 문제는 하기의 단락들에 기재된 바와 같은 전사물 변이체 경우의 정렬 단계이다. 짧은 서열 리드를 하나의 전사물 변이체에 정확하게 정렬시키고 특히 샘플 중에 존재하는 모든 전사물 변이체를 신뢰성 있게 정량분석하는 것은 대개 어렵다.
EP 2 333 104 A1은 잠재적으로 다양한 RNA 분자들의 풀로부터 유래된 핵산 분자 단편 서열의 RNA 분석학적 배열 방법에 관한 것이다. 유전자는 하나의 전사물 변이체에서 발현될 뿐만 아니라 다수의 전사물 동형(isoform)들이 주어진 게놈 영역으로부터, 그들의 엑손-인트론 조성 및 전사 개시-(TSS) 및 종료-부위(TES)의 변화와 함께 전사될 수 있다(예를 들어 Nilsen and Graveley, 2010; Wang et al., 2009; Koscielny et al., 2009을 참조하시오). 전사물 동형들은 또한 여섯 자릿수(106 배)까지 그들의 풍부성이 다양할 수 있으며, 이는 추가적인 복잡성을 한 층 더 가중시킬 수 있다(Aird et al., 2013). 장(Zhang) 등은 합성적인 선택적 스플라이싱 데이터베이스에 관한 것이다.
RNA-Seq에 의한 상기 전사체의 복잡성 분석은 짧은 리드를 어노테이션된(annotated) 참조 게놈에 정렬시키고 전사물 유사체 및 가설을 특유의 특징, 예를 들어 콘틱 커버리지(contig coverage)로부터 찾아 엑손-엑손 접합을 증명함을 필요로 한다(예를 들어 Wang et al., 2009를 참조하시오). 이러한 연산(algorithms)은 불충분하고 상이하게 관장된 어노테이션, 및 유사한 특징을 공유하고 유사한 수준으로 발현되는 전사물 변이체를 식별하는 본질적인 문제로 인해 결코 정확하지 않다. 게놈 서열 및 어노테이션을 사용하지 않는 전사체 드노보 조립(transcriptome de novo assembly)은 훨씬 더 어려우며 비효율적이고 대개는 충분히 특성화되지 않은 유기체에 적용된다.
본 발명의 목적은 샘플 중 전사물 변이체의 보다 정확한 평가(즉 식별 및 정량분석)를 허용하는 방법 및 생성물을 제공하는 것이다.
본 발명은
a) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 상이한 핵산 (NA) 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지는, 전사물 변이체를 모방하는(simulating) 인공 NA 분자의 참조 세트를 제공하고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 뉴클레오티드(nt) 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하며,
여기에서 상기 NA 분자의 적어도 2개, 바람직하게는 각각은 소정의 몰량으로 존재하는 단계; 및
b) 상기 참조 세트를 외부 대조용으로서 상기 전사물 변이체를 포함하는 하나 이상의 샘플에 가하는 단계; 및
c1) NA 서열분석을 리드 생성(read generation) 및 할당(assignment)을 근거로 수행하고, 여기에서 참조 리드 할당은 상기 참조 세트의 리드에 의해 생성되고 상기 참조 리드 할당을 사용하여 상기 하나 이상의 샘플의 전사물 변이체의 리드 할당을 조절, 확인 또는 변형시키는 단계; 또는
c2) NA 검출 또는 정량분석 방법, 바람직하게는 마이크로-어레이 분석 또는 qPCR을 상기 하나 이상의 샘플상에서 수행하고, 여기에서 적어도 하나의 탐침이 상기 참조 세트의 적어도 하나의 NA 분자에 결합하며 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 적어도 하나의 탐침으로부터 생성되는 신호에 기반한 측정 결과를 사용하여 상기 NA 검출 또는 정량분석 방법에서 탐침에 결합하는 상기 하나 이상의 샘플의 전사물 변이체로부터 생성되는 신호에 기반한 측정 결과를 조절, 확인 또는 변형시키는 단계
를 포함하는, 하나 이상의 샘플 중 전사물 변이체의 조절된 식별 및/또는 정량분석 방법을 제공한다.
본 발명은 또한 상기 방법에 사용하기에 매우 적합한 인공 NA 분자의 참조 세트뿐만 아니라, 상기와 같은 참조 세트 및 상기와 같은 참조 세트 중에 함유하기에 적합한 NA 분자를 생성시키는 방법을 제공한다.
하기의 상세한 설명 및 바람직한 실시태양을 본 발명의 모든 태양에 적용하며, 명백히 지시되는 경우를 제외하고, 제한 없이 서로 조합할 수 있다. 바람직한 실시태양 및 태양을 특허청구범위에서 추가로 정의한다.
내부, 외부, 상대적 및 절대적 표준은 전사물 변이체(진핵생물 세포로부터의 거의 모든 전사물 샘플에 적용된다)를 포함하는 샘플의 상이한 정성적 지표를 측정하고 상기와 같은 복잡한 전사물 샘플을 분석하고자 하는 방법에 필수적이다. 정량적 데이터를 상대적인 또는 절대적인 용어로 나타낼 수 있다. 각각의 다양한 방법, 예를 들어 마이크로-어레이, qPCR 또는 NGS는 측정 결과의 표준화에 관하여 상기 데이터 분석에서 다수의 특수성을 갖는다.
마이크로-어레이 및 qPCR에서의 상대적인 정량분석을 위해서 RNA 수준을 내부 또는 외부 대조용을 사용하여 샘플들간에 비교하여 샘플 농도 및 부하에서의 차이를 표준화한다. NGS 실험은 리드의 수, 및 식별된 전사물의 길이에 대해 상이한 표준화 과정을 사용한다. 상기 결과는 유전자 어노테이션의 품질 및 상태와 같은 다수의 변수, 또는 상기 정렬 및 조립 연산에 의한 라이브러리 제조와 서열분석 편향간의 일치에 따라 변한다. 예를 들어 상기 라이브러리 제조 효율에서의 차이를 보상하기 위한 대조군이 필요하다.
대조군은 상기 샘플 세트 전체 걸쳐 일정한 수준으로 발현되는 유전자(내부 참조) 또는 스파이크-인(spike-in))되는 RNA(외부 참조)이다. 정량분석의 경우 상기 실험 유전자, 엑손 또는 태그의 발현 수준을 나타내는 신호 강도(형광 단위 또는 리드 수)는 기지의 양 또는 비를 함유하는 표준과 관련되며 절대적인 또는 상대적인 참조로서 정의되었다.
상기 US 2004/009512 A1은 내부 대조용 탐침을 사용하여 mRNA 스플라이싱 생성물을 분석하는 방법을 개시한다(청구항 7, 상기 문서의 [0097] 및 [0106]의 단락). 본 발명과 관련된 분자의 길이를 갖는 변이체를 나타내는 내부 대조군에 관한 개시는 없다.
다수의 복잡한 RNA 표준 샘플, 예를 들어 보편적인 인간 참조 RNA 및 보편적인 인간 뇌 참조 RNA(앰비온(Ambion), 라이프 테크놀로지스(Life Technologies))를 상업적으로 입수할 수 있다. 상기 표준을 다수의 공여자 및 다수의 조직/뇌 영역으로부터 모으며(pooled), 따라서 상기 표준은 광범위하게 편향되지 않고 재현 가능한 유전자 발현 커버리지를 목표로 한다. 상기와 같은 표준 샘플상에서의 실험은 참조 데이터를 제공하며 실험 방법을 입증하고 평가하는데 사용된다. 미지의 샘플에 대한 측정을 서로 및 상기 표준 샘플에 대해 연동시키기 위해서 내부 또는 외부 표준이 필요하다.
내부 RNA 표준은 분석되는 샘플 전체에 걸쳐 비교적 일정한 수준으로 발현되는 유전자이다. 내부 표준은 유기체의 상이한 조직들에서, 모든 발생 단계에서, 및 대조용 및 실험적으로 처리된 세포 유형에 대해 균등하게 발현되어야 하며, 이를 종종 "하우스키핑" 유전자라 칭한다. 불행하게도, 18S rRNA가 가장 광범위한 실험 조건하에서 이상적인 내부 대조군에 가까운 것으로 보이지만, 상기 모든 상황에서 일정한 발현 수준을 갖는 단일 RNA는 존재하지 않는다. 그러나, rRNA의 비교적 높은 풍부성은 rRNA를 자유로운 서열분석 공간으로 특이적으로 소모시키는 라이브러리 제조 방법을 도출한다.
따라서, 특정한 실험 문제에 대해서 가장 있음직한 mRNA가 되는 적합한 대조용 RNA를 식별할 필요가 있다. 이는 차례로 상기 표준의 적합성에 대한 mRNA 동형들의 영향을 고려할 필요가 있다. 일부 내부 표준을 찾을 수 있지만(β-액틴, 글리세르알데히드-3-포스페이트-데하이드로게나제(GAPDH), 또는 사이클로필린 mRNA), 오직 외부 표준만이 조절되고 신뢰성 있는 참조 값을 제공한다. 다른 종의 RNA 샘플로부터의 일정한 공급원, 예를 들어 포유동물 샘플에 첨가된 세균 전사체를 외부 표준으로서 사용할 수 있었다. 그러나, 원핵생물처럼 훨씬 더 단순한 유기체들이 이미 상기와 같은 높은 전사물 수를 갖기 때문에, 전체 동적(농도) 범위 전체를 통해 균형잡힌 표시는 너무 많은 서열분석 공간을 낭비할 것이다. 따라서, 복잡성은 낮지만 필적하는 동적 범위를 갖는 외부 표준, ERCC가 앞서 개발되었다.
국립 표준기술 연구소(NIST, USA)에 의해 선도되고 37개 연구소로 이루어지는 ERCC 협회는 함께 합성 DNA 서열, 또는 바실러스 서브틸리스(Bacillus subtilis) 또는 심해 배출 미생물 메타노칼도코커스 자나쉬이(Methanocaldococcus jannaschii) 게놈으로부터 유래된 DNA의 시험관내 전사에 의해 대조용 RNA를 합성하였다. 상기 전사물은 모노엑손이고 비-동형성으로 되어 있다, 즉 상기는 스플라이싱 또는 다른 전사물 변이체를 나타내지 않는다. 상기 협회는 19 내지 25 아데닌(중간 23 아데노신)의 폴리-A 꼬리 길이, 250 내지 2000 nt의 길이 및 ∼30 내지 55%의 GC-함량을 결정하였다. 상기 다양한 서열은 내인성 전사물의 성질들 중 적어도 일부, 예를 들어 GC 함량 및 길이의 다양성을 나타낸다. ERCC RNA는 서열분석된 진핵생물로부터의 내인성 전사물과 최소의 서열 상동성을 나타낸다(External RNA Controls Consortium, 2005a). 상기 ERCC 믹스 개발이 특별 보고서(External RNA Controls Consortium, 2005)에 기록되어 있다.
블롬퀴스트(Blomquist) 등은 NGS에 의한 DNA 서열분석에 관한 것이며 합성 내부 표준을 사용하는 방법을 사용한다(상기 문서의 초록 및 도 1). RNA 가공 중에, ERCC 스파이크-인 대조용 내부 표준이 사용된다(상기 문서의 p.4, 좌측 컬럼). 데븐셔(Devonshire) 등이 또한 상기 ERCC에 관한 것이다.
앰비온(라이프 테크놀로지스의 파트)은 상업적으로, 자립형(stand-alone) 믹스(106배에 걸친 농도) 또는 차별적인 유전자 발현을 위해 비교해야 하는(변화 배수 측정의 정확도를 측정하는; 사용자 지침: ERCC RNA Spike-In Control Mixes, Ambion) 2개 샘플에 스파이크되도록 설계된 2개의 믹스로 92개 ERCC 전사물을 제공한다.
상기는 처음에 qPCR 및 마이크로-어레이 시스템에 사용되는 것으로 고안되었지만, 현재는 RNA-Seq NGS 실험에 널리 사용되고 있다. 상기 상이한 의도적인 목적은 ERCC의 현행 용도를 의심스럽게 만든다.
상기 ERCC의 한계는 i) 그의 크기 범위가 제한되고, ii) 오직 짧은 폴리-A-꼬리만을 함유하며, iii) 캡-구조가 없다는 것이다. 그러나, 상기 ERCC의 주요 단점은 상기가 어떠한 종류의 전사물 변이체도 함유하지 않는다는 것이다. 따라서, 상기는 이 점에 있어서 전사물 변이체의 조절된 식별 및/또는 정량분석 및 서열분석 방법(또는 다른 분석 방법)의 평가에 적합하지 않다. 또 다른 단점은 상기가 공지된 서열(바실러스 및 메타노코커스)과 유사성을 갖는다는 것이다.
선(Sun) 등은 선택적으로 스플라이싱된 전사물의 정량분석에 관한 것이다. 인간 텔로머라제 역전사효소의 스플라이싱 변이체가 대조용으로서 사용된다. 대략 20개의 스플라이싱 변이체가 공지되어 있으며, 이중 4개는 종양에 통상적이다(상기 문서의 p. 319, 중간 컬럼). 상기 통상적인 4개는 상기 문서에서 조사되었다(p.320 중간 컬럼, 및 도 1; p. 321, 좌측 컬럼; 상기 문서의 표 1). 그러나, 상기 문서는 인공 전사물 변이체에 관한 것이 아니며, 상기 문서의 대조군은, 천연 서열에 의존할 필요 없이(천연 서열에 대한 의존은 실제로 상기 실험을 방해할 수 있다) 선택적 스플라이싱 사건의 전형적이고 정확한 모방을 허용하는 본 발명과 달리, 단일 인간 유전자로 제한된다.
본 발명은 특히 이러한 단점들을 극복한다. 본 발명의 과정에서, 다수의 상이한 방법 및 참조 세트들이 전사물 변이체의 식별 및 정량분석의 현행 문제를 해결하기에 특별히 적합한 방법 및 생성물을 찾아내기 위해서 개발되고 특성화되었다.
따라서, 본 발명의 하나의 태양에서,
a) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 상이한 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지는, 전사물 변이체를 모방하는 인공 핵산(NA) 분자의 참조 세트를 제공하고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 뉴클레오티드(nt) 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하며,
여기에서 상기 NA 분자의 적어도 2개, 바람직하게는 각각은 소정의 몰량으로 존재하는 단계(이는 예를 들어 상기 참조(즉 대조용) 리드 할당에 대한 상기 샘플 리드 할당의 표준화를 허용하기 때문에 상기 참조 세트를 본 발명에 특히 적합하게 한다);
b) 상기 참조 세트를 외부 대조용으로서 상기 전사물 변이체를 포함하는 하나 이상의 샘플에 가하는 단계(상기 참조 세트를 동일한 샘플 용기(들) 및/또는 별도의 분석용 용기에 물리적으로 가할 수 있다. 또한, 또는 한편으로, 상기를 또한 동일한 분석 장비, 동일한 분석 장비 모델 또는 다른 분석 장비 모델로부터 상기 참조 세트의 선행 측정을 사용함으로써 컴퓨터-실행되는 방법 단계에 비-물리적으로 가할 수 있다);
c1) NA 서열분석을 리드 생성(상기 리드는 임의의 길이를 가질 수 있다) 및 할당(즉 상기 리드의 참조 서열상으로의 지도화)을 근거로 수행하고, 여기에서 참조 리드 할당은 상기 참조 세트의 리드에 의해 생성되고 상기 참조 리드 할당을 사용하여 상기 하나 이상의 샘플의 전사물 변이체의 리드 할당을 조절, 확인 또는 변형시키는 단계; 또는
c2) NA 검출 또는 정량분석 방법, 바람직하게는 마이크로-어레이 분석 또는 qPCR을 상기 하나 이상의 샘플상에서 수행하고, 여기에서 적어도 하나의 탐침이 상기 참조 세트의 적어도 하나의 NA 분자에 결합하며 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 적어도 하나의 탐침으로부터 생성되는 신호에 기반한 측정 결과를 사용하여 상기 NA 검출 또는 정량분석 방법에서 탐침에 결합하는 상기 하나 이상의 샘플의 전사물 변이체로부터 생성되는 신호에 기반한 측정 결과를 조절, 확인 또는 변형시키는 단계
를 포함하는, 하나 이상의 샘플 중 전사물 변이체의 조절된 식별 및/또는 정량분석 방법을 제공한다. qPCR에서, 상기 탐침은 PCR 반응에서 연장되는 프라이머 또는 표지된 DNA 탐침일 수 있으며; 마이크로-어레이 분석에서 상기 탐침은 DNA 칩상에 고정화된 DNA 탐침일 수 있다.
상기 NA는 DNA 또는 RNA일 수 있다. 바람직하게, 상기는 RNA이다.
당해 분야의 숙련가는 DNA 또는 RNA로서 상기 참조 세트를 적용해야 할 때 선택에 자유롭다. 숙련가는 또한 NA 서열분석 또는 NA 검출 또는 정량분석 방법을 위해 샘플을 어떻게 제조해야 하는지를 알고 있다. 이롭게는, 상기 참조 세트를, 상기 참조 세트가 모든 또는 대부분의 샘플 제조 단계 중에 존재하도록 NA 서열분석 또는 NA 검출 또는 정량분석 방법을 적용하기 전 샘플 제조 중에 초기에 가한다. 이를 위해서, 바람직하게는 상기를, 상기 전사물 변이체(관심 분자)가 상기 샘플 제조 중 초기에는 전형적으로 mRNA 분자이므로, RNA로서 가한다.
"인공 NA 분자" 또는 "인공 유전자" 또는 "인공 서열"에서와 같은 "인공"이란 용어는 문서 전체를 통해 사용되는 바와 같이, 상기 존재가 천연 생물 유기체(예를 들어 미생물, 동물 또는 식물) 중에 존재하지 않고 인간에 의해 의도적으로 고안되어 생성된 인공물로서 지칭됨을 의미한다. 그러나, 인공 NA 분자 또는 인공 유전자와 같은 인공적인 존재는 여전히 인공적인 그의 특성을 상실하지 않으면서 재조합 유기체에서 생성될 수 있다(예를 들어 천연 이 콜라이 세포내로 도입되어 발현된다).
인공 NA 분자는, 특히 상기가 공지된 NA 서열에 대해 서열 상동성이 없거나 또는 단지 무시할 만한 서열 상동성을 갖는 경우, 본 발명의 방법에 특별히 매우 적합하다. 이는 차세대 서열분석 리드에 전형적인 짧은 서열(예를 들어 40 내지 80 nt 또는 심지어 20 내지 200 nt)의 경우에조차 "참조 리드"로서 리드의 명확한 할당을 허용한다.
일반적으로, 전사물은 전사 개시 부위에서부터 전사 종료 부위에 달하는 RNA 서열로 이루어지는 하나의 유전자로부터(예를 들어 DNA 주형으로부터)의 전사 생성물(예를 들어 RNA 폴리머라제에 의해 합성된)이다. 본 발명의 목적을 위해서, 전사물은 적어도 하나의 엑손을 포함하는 NA 분자이다. 전사물이란 단어는 단일 분자 또는 동일한 서열을 갖는 모든 분자의 그룹을 기술한다. 주지된 바와 같이, 진핵생물에서 mRNA(전사물)는 성숙한 전사물이 되도록 전-mRNA(또한 이종 핵 리보핵산으로서 지칭된다)로부터 가공된다(특히 스플라이싱에 의해). 정의에 따라, 상기 전사물 밖으로 스플라이싱되는 서열 영역을 인트론이라 칭하며, 성숙한 전사물 중에 남아있는 서열 영역을 엑손이라 칭한다. 하나의 성숙한 전사물 변이체에서 엑손은 또 다른 성숙한 전사물 변이체에 대한 인트론일 수 있다(상기 변이체에 존재하지 않음으로 인해). 유전자 서열 영역을, 모든 전사물 변이체의 서열들이 공지된 경우, 엑손 및 인트론으로서 어떻게 어노테이션하는지는 숙련가에게 명백하다. 본 명세서에 사용되는 바와 같이, 엑손은 임의의 변이체에서 엑손일 수 있는 서열 영역이다. 상기는 대개 동봉된 인트론 영역의 양쪽 단부에서 다소 보존된 서열을 통해 특성화되며 이웃하는 엑손과 소위 엑손-엑손 접합을 형성한다(또한 표 2를 참조하시오). 천연 엑손은 암호화 영역의 부분(또는 이와 역)일 수 있으나, 본 발명의 인공 NA 분자의 경우에 상기 엑손은, 본 발명의 인공 서열이 천연으로 존재하는 유기체 중에 존재하는 공지된 전사물과의 유사성이 결여되도록 설계되고 개시 및 정지 코돈을 갖는 판독 프레임 또는 오직 개시 코돈만을 갖는 개방 판독 프레임(ORF)을 함유하지 않기 때문에, 바람직하게는 인공 단백질 또는 천연 단백질에 대한 암호화 영역의 부분(또는 이와 역)이 아닐 수 있다. 본 발명의 인공 NA 분자 중에 포함된 엑손은 인공 서열을 포함하기 때문에 인공 엑손이다. 본 명세서에서 "전사물"이란 단어는 달리 서술되지 않는 한, "성숙한 전사물"을 의미하는 것으로 해석될 것이다.
가장 광범위한 용어로, 전사물 "변이체"는 유전자의 전사물이며, 여기에서 상기 유전자의 적어도 2개의 전사물이 존재하고, 상기 전사물은 상기 적어도 2개의 전사물의 다른 하나와 적어도 하나의 뉴클레오티드("선택적인 전사 사건"에 의해 생성됨)만큼 상이하다. 그러나, 본 발명의 방법과 관련하여, 각 (전사물) 그룹의 인공 NA 분자는, 각 그룹에 대해 독립적으로, 적어도 80 뉴클레오티드 길이(바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt)의 서열을 공유하며, 각 그룹에 대해 독립적으로, 각 그룹의 적어도 2개의 NA 분자는 적어도 80 뉴클레오티드 길이(바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt)의 적어도 또 다른 서열만큼 상이하다. 상기 그룹의 다른 구성원들은 단지 하나의 뉴클레오티드만큼 추가의 구성원들과 상이할 수 있지만, 변이체들 간에 더욱 큰 차이, 예를 들어 상기 그룹의 모든 구성원들간에 단지 80 nt 또는 100 nt 또는 150 nt 또는 200 nt 신장부 이하의 서열 일치성이 바람직하다.
본 명세서에서, (인공 유전자의) "전사물 변이체를 모방하는"은 천연 진핵생물(바람직하게는 동물 또는 식물, 보다 바람직하게는 척추동물, 훨씬 더 바람직하게는 포유동물, 특히 인간) 유전자의 천연 진핵생물(바람직하게는 동물 또는 식물, 보다 바람직하게는 척추동물, 훨씬 더 바람직하게는 포유동물, 특히 인간) 전사물의 전형적인 특징을 가짐을 의미한다. 당해 분야의 숙련가는 전사물 변이체의 상기 전형적인 특징과 친숙하다. 이러한 특징은 하기 중 하나 이상을 포함한다: 하나 이상의 선택적인 스플라이싱 사건(하기 및 표 1 참조)의 결과이고, 몇몇 인트론 스플라이싱 부위 디뉴클레오티드(하기 및 표 2 참조)를 갖고, 선택적 전사물 개시- 및 종료-부위(하기 참조)를 갖고, 인티센스 전사물이고, 다른 유전자/전사물과 중복되고, 폴리아데닐화된다(또한 Wang et al., 2008 참조). 추가로, 또는 선택적으로, 왕(Wang) 등(2008), 특히 도 2에 정의된 특징들을 사용할 수 있다. 이롭게, 상기 참조 세트의 NA(RNA 또는 DNA) 분자 전체는 선행 2개의 문장에 인용된 특징들 중 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 4개, 특히 적어도 5개를 가지며, 이때 각각의 NA 분자는 서로 독립적으로, 적어도 하나 또는 적어도 2개 또는 적어도 3개 또는 적어도 4개의 별도의 경우에, 선행 문장에 인용된 특징들 중 어느 것도 갖지 않거나, 또는 1, 2, 3, 4, 5 또는 6개를 갖는다. 본 발명의 NA 분자는 전사물 변이체를 모방하기 위해서 RNA 분자일 필요는 없다. 전사물 변이체의 모방은 DNA 또는 다른 NA 분자에 의해서 또한 가능하다.
본 발명의 목적을 위해서, 인공 유전자를 포함하는 인공 게놈을 생성시킬 수 있다(개념상 컴퓨터에서, 서열들을 배열시킴으로써). 상기 인공 게놈의 서열을 또한 리드 할당에 사용할 수 있다. 인공 유전자는 천연 유전자로부터 공지된 특징, 예를 들어 프로모터, 전사 개시 부위, 전사된 영역 및 전사 종료 부위(또한 종결자라 지칭됨)를 갖는다. 상기 프로모터 영역은, 본 발명이 인공 유전자의 전사 변이체를 모방함에 관한 것이기 때문에(그리고 상기 인공 유전자 또는 상기 인공 유전자 자체로부터 상응하는 단백질의 합성이 아니기 때문에), 본 발명의 목적과 무관하다. 동일한 인공 유전자의 참조 전사물 변이체인 인공 NA 분자(즉 인공 NA 분자 그룹의 일원)는, 동일한 천연 유전자의 천연 전사물이 서로 및 상기 천연 유전자와 관련되는 바와 동일한 방식으로, 서로 및 상기 인공 유전자와 관련된다(크기 및 서열과 같은 매개변수에 의해). 이들의 공통적인 특징은 전사물 변이체가 동일한 가정 유전자로부터 전사되는 변이체들 간에 엑손(또는 그의 부분)을 공유하는 것일 수 있다. 본 발명의 목적을 위해서 상기 인공 유전자가 상기 인공 NA 분자를 한정하는 단순한 개념이며 상기 인공 NA 분자의 정의에 필요한 것 이상으로 한정될 필요는 없음은 숙련가에게 명백하다(예를 들어 이전에 언급한 바와 같이, 상기 유전자의 프로모터 영역을 한정할 필요는 없다).
이롭게, 전사물 변이체를 모방하는 인공 폴리핵산 NA(RNA 또는 DNA)의 참조 세트는 적어도 하나 또는 적어도 2개 또는 적어도 3개 또는 적어도 4개의 별도의 경우에, 상기 참조 세트 중에 존재하는 전형적인 전사물 특징 중 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 상기 전형적인 전사물 특징 중 적어도 3개, 특히 적어도 4개, 특히 전부에서, 진핵생물(바람직하게는 동물 또는 식물, 보다 바람직하게는 척추동물, 훨씬 더 바람직하게는 포유동물, 특히 인간) 전사체의 전형적인 전사물 특징(예를 들어 하기의 단락들에서 명시되는 바와 같은)의 상응하는 평균 빈도와 유사한(적어도 +/-50%, 바람직하게는 적어도 +/-25%, 특히 적어도 +/-10%) 선행 단락들에서 언급된 전형적인 전사물 특징의 빈도를 갖는다.
선택적 스플라이싱 사건(AS):
선택적 스플라이싱란 용어는 생물학에서 1차 전사물(전-mRNA)을 하나보다 많은 패턴으로 스플라이싱하여 다수의, 별개의 성숙한 mRNA를 생성시킬 수 있는 임의의 경우를 기술하는데 사용된다. 선택적 스플라이싱 사건의 가장 통상적인 유형들을 표 1에 나타낸다. 인간에서, 엑손 스키핑은 발견되는 가장 통상적인 스플라이싱 사건의 ∼33%를 차지한다. 선택적인 5' 및 3' 스플라이싱 부위가 각각 ∼25%로 이어진다. 또한, 선택적 스플라이싱 부위는 종종 함께 존재한다(Barbazuk et al., 2008; Roy et al., 2013). 뇌 조직 및 고환 조직은 다수의 AS 사건들을 갖는 것으로 밝혀졌다(Roy et al., 2013). 이롭게, 상기 참조 세트의 NA 분자 전체는 표 1에 인용된 특징들 중 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 4개, 특히 적어도 5개를 가지며, 이때 각각의 NA 분자는 서로 독립적으로 적어도 하나 또는 적어도 2개 또는 적어도 3개 또는 적어도 4개의 별도의 경우에, 선행 문장에서 인용된 특징들이 없거나, 상기 특징들 중 1, 2, 3, 4, 5, 6 또는 7개를 갖는다.
선택적 스플라이싱 사건. 목록은 Ensembl 유전자 어노테이션으로부터 유래된 다수의 선택적 스플라이싱 사건들을 나타낸다. 상기 Ensembl 유전자 세트는 자동 및 수동 어노테이션을 포함하며, 이때 모든 전사물은 실험 증거에 기반한다(또한 Wang et al., 2008을 참조하시오).
AS 패턴 유형 약어 정의
카세트 엑손
( 스키핑된 엑손) 		CE 하나의 엑손이 그의 인접하는 인트론과 함께 1차 전사물 밖으로 스플라이싱된다.
인트론 유지 IR 하나의 서열이 인트론으로서 스플라이싱되거나 성숙한 mRNA 전사물 중에 남아있는다.
상호 배타적인 엑손 MXE 다수의 카세트 엑손이 상호 배타적인 방식으로 사용되는 경우를 지칭한다. 가장 간단한 경우: 성숙한 mRNA 전사물 중에 결코 2개가 모두 포함되지 않는 2개의 연속적인 엑손.
선택적인 3' 부위 A3SS 선택적인 수용체 부위를 또한 지칭한다. 2개 이상의 스플라이싱 부위가 엑손의 5' 단부에서 인식된다. 선택적인 3' 스플라이싱 접합(수용체 부위)이 사용되어, 하류 엑손의 5' 경계를 변화시킨다.
선택적인 5' 부위 A5SS 선택적인 공여체 부위를 또한 지칭한다. 2개 이상의 스플라이싱 부위가 엑손의 3' 단부에서 인식된다. 선택적인 5' 스플라이싱 접합(공여체 부위)이 사용되어, 상류 엑손의 3' 경계를 변화시킨다.
선택적인 첫 번째 엑손 AFE 각 변이체의 두 번째 엑손이 동일한 경계를 가지나, 제1 엑손은 상호 배타적이다. 이는 가능한 선택적 프로모터 사용을 어노테이션한다.
선택적인 최종 엑손 ALE 각 스플라이싱 변이체의 끝에서 두 번째 엑손이 동일한 경계를 가지나, 최종 엑손은 상호 배타적이다. 이는 가능한 선택적인 폴리아데닐화 사용의 어노테이션을 허용한다.
안티센스 전사물 및 중복 유전자:
중복 변이체뿐만 아니라 모노엑손 안티센스 전사물이 유전자 부분집합에 대해서 모든 전사물의 상당한 몫(인간에서 9%, 마우스에서 7.4%; Sanna et al., 2008)을 구성하는 것으로서 설계되었다. 상기 중복 변이체는 모노엑손이거나 센스 또는 안티센스 방향으로 스플라이싱될 수 있다(예를 들어 단지 말단 엑손 중복만을 갖는 3 엑손). 안티센스-배향된 유전자는 같은 방향의 중복 유전자보다 10배 더 빈번할 수 있다. 이롭게, 상기 참조 세트의 NA 분자 전체는 센스 및/또는 안티센스 방향으로 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개의 중복 전사물을 포함한다. 바람직하게, 상기와 같은 전사물의 빈도는 상기 참조 세트 중에 존재하는 모든 전사물의 약 10%이다. 2개의 인공 전사물 변이체간의 안티센스 중복은 예를 들어 10 nt 내지 500 nt의 길이일 수 있다.
선택적인 전사물 개시- 및 종료-부위(TSS 및 TES):
선택적인 첫 번째 및/또는 최종 엑손(AFE 및 ALE)을 생성시키는 선택적 스플라이싱 사건 외에, 또한 어노테이션된 엑손내 또는 엑손 전체에 상기 전사물의 실제 개시 또는 종료 부위가 가능하다. 미세-변화의 경우, 상기 어노테이션된 부위로부터의 정확한 편차는 논쟁의 여지가 있지만 대개는 <20 nt이다. 더욱이, 이들은 기능상 유사하다, 즉 동일한 프로모터 또는 동일한 폴리아데닐화 신호에 따르며, 따라서 이들은 조정시 함께 달라진다. 큰 변화의 경우, 이들 선택적인 TSS 및 TES는 전형적으로 선택적인 프로모터 또는 폴리아데닐화 신호에 따라 변하며 동일한 첫 번째 또는 최종 엑손내에 또는 이웃하는 것들 중에 위치할 수 있다. 이들은 더 떨어져 위치한다, 즉 프로모터들에 대한 참조 거리로서 500 nt가 고려될 수 있으며(Xin et al., 2008) 폴리-A 부위 측량시 조정 거리로서 40 nt가 관찰되었다(Yoon et al., 2012). 따라서, 이롭게, 상기 참조 세트의 NA 분자 전체는 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개의 TSS 및/또는 TES를 포함한다. 바람직하게, 하나의 그룹에서 적어도 2개의 전사물 변이체는 20 nt, 바람직하게는 10 nt의 긴 5' 또는 3' 말단 영역에서 적어도 1 nt, 바람직하게는 2 nt, 3 nt, 4 nt, 5 nt 이상 만큼 상이하다.
본 명세서에서, 선택적인 스플라이싱 사건, 선택적인 전사물 개시- 및 종료-부위 및 안티센스 전사물 및 중복 유전자를 "선택적인 전사 사건"이란 용어 하에 포함시킨다.
인트론 스플라이싱 부위 디뉴클레오티드:
대부분의 인트론은, 스플라이시오솜 성분에 의해 인식되고 스플라이시오솜 형성에 필요한 5' 및 3' 단부 부근의 공통의 공통 서열(consensus sequence)을 갖는다(도 1). 주요 부류의 경우, 스플라이싱 접합쌍은 고도로 보존되며 전형적으로 인트론 공여체 및 수용체 서열 GT-AG(어노테이션된 접합의 98.70%)를 포함하며, 이어서 GC-AG 및 AT-AC의 빈도로 이어진다(표 2). 보다 일반적인 관점에서, 가장 통상적인 엑손-인트론 서열을 엑손...AT(컷)GT...인트론...AG(컷)G... 다음 엑손으로서 묘사할 수 있다. 표 2에서, 공여체-수용체 쌍의 빈도를 제공한다. 이러한 보존 및 적당한 변이성을 밝히기 위해서, 모든 접합의 97%가 GT-AG이고, 2%는 GC-AG이며 1%는 AT-AC인 것을 목표로 하였다. 이러한 모방은 정렬자(aligner)(예를 들어 톱햇(TopHat))가 그의 기존의 접합 표를 사용하여 평가할 수 있게 해야 한다. 엑손 경계는 상기가 보다 중요한 인트론 접합 디뉴클레오티드를 방해하지 않는 5' AG 및 3' AT이어야 한다. 이롭게, 상기 참조 세트의 NA 분자 전체는 바람직하게는 각각 존재하는 모든 인트론 공여체-수용체 디뉴클레오티드의 약 97%, 2% 및 1%의 빈도로 GU-AG, GC-AG, AU-AC 중에서 선택된 바와 같은 엑손의 하나, 바람직하게는 2개, 특히 모든 인트론 공여체-수용체 디뉴클레오티드를 포함한다.
규정 및 비규정 공여체 수용체 쌍. 10,803개 인간 유전자에 대한 게놈 스플라이싱 부위(SS)의 엑손 좌표에 대한 정보(ICE) 데이터베이스로부터 유래된 스플라이싱 부위 디뉴클레오티드. 공여체 및 수용체 디뉴클레오티드의 256개의 이론적으로 가능한 쌍으로부터, 3개의 가장 전형적인 특정한 쌍(GT-AG, GC-AG 및 AT-AC)이 모든 경우의 99.56%를 차지한다(91,846 중 91,022)(Chong et al., 2004).
# 디뉴클레오티드 분율 # 디뉴클레오티드 분율
1 GT -AG 98.70% 12 AT-AG 0.02%
2 GC -AG 0.79% 13 GC-CT 0.02%
3 AT - AC 0.08% 14 GT-CT 0.02%
4 GT-GG 0.06% 15 GT-TT 0.02%
5 GG-AG 0.04% 16 AG-AG 0.02%
6 GA-AG 0.03% 17 GC-GG 0.02%
7 GT-TG 0.03% 18 GC-TG 0.02%
8 AT-AT 0.03% 19 GT-GA 0.02%
9 GC-CA 0.03% 20 CA-AG 0.01%
10 GT-AT 0.02% 21 CC-AT 0.01%
11 AA-AG 0.02% 22 GG-CA 0.01%
폴리아데닐화:
성숙한 진핵생물 전사물은 폴리-A 꼬리를 갖는 것으로 공지되어 있다. 이롭게, 본 발명의 또는 본 발명의 방법에 사용하기 위한 인공 NA 분자는 적어도 10, 바람직하게는 적어도 20, 특히 적어도 30 아데노신의 폴리-A 꼬리를 가지며, 이는 실제 전사물의 가까운 모방을 지원한다. 또한, 상기는 (특히 적어도 30 아데노신의 경우) 적합한 올리고(dT) 비드 정제를 보장하며, 또한 모든 구조물의 보편적인 증폭을 위한 T7-프로모터 및 폴리-A 결합 프라이머와의 PCR 증폭 반응에서 5'/3' 프라이머 융점(Tm)의 균형을 돕는다.
본 발명의 상기 방법은 바람직하게는 리드 생성(상기 리드는 임의의 길이를 가질 수 있다) 및 할당(즉 상기 리드를 참조 서열상에 지도화한다)을 근거로 NA 서열분석을 수행함을 포함하며, 여기에서 참조 리드 할당은 상기 참조 세트의 리드에 의해 생성되고 상기 참조 리드 할당을 사용하여 하나 이상의 샘플의 전사물 변이체의 리드 할당을 조절, 확인 또는 변형시킨다. 상기 리드 할당을 조절, 확인 또는 변형시키기 위해서 외부 대조군을 어떻게 사용하는지는 당해 분야에 공지되어 있다(예를 들어 Jiang et al., 2011).
본 발명의 과정에서, 용기 중에 건조 형태로(예를 들어 상기 샘플 자체에 의해 용해되는) 인공 NA 분자의 참조 세트를 제공함은 취급 오차를 감소시키는 것으로 밝혀졌다(또한 실시예 8을 참조하시오). 또한, NA 분자(특히 RNA 분자)는 전형적으로 건조시 보다 안정성이다. 따라서, 특히 바람직한 실시태양에서, 인공 NA 분자의 참조 세트를 용기 중에 건조하게, 바람직하게는 동결-건조시켜 제공한다. 전형적으로, 참조 세트를 갖는 별도의 용기를 각 샘플에 제공한다. 바람직하게, 안정화제(NA, 특히 RNA의 분해를 감소시키는)를 상기 참조 세트에, 상기 건조 전, 건조 도중 또는 건조 후에, 특히 건조 전에 가한다. 상기와 같은 안정화제는 산화방지제, EDTA, DDT, 다른 뉴클레아제 또는 RNAse 억제제(예를 들어 프로메가(Promega)의 RNAsin®, 바이오매트리카(Biomatrica)의 RNAstable®, 젠테그라(GenTegra)의 젠테그라®-RNA)를 포함한다. 전형적으로, DNA 분자보다 RNA 분자에 추가적인 안정화가 더 중요하다.
선행 단락에 따라, 또 다른 매우 바람직한 실시태양에서, 외부 대조군으로서 상기 참조 세트의 첨가를, 상기 샘플을 상기 용기에 가함으로써 수행하여 상기 샘플 중에 건조된 참조 세트를 용해시킨다.
하기는 상기 하나 이상의 샘플의 전사체 변이체의 리드 할당을 어떻게 조절, 확인 또는 변형시키는가에 대한 일례를 기재한다: 본 설정에서 유전자 1(G1)은 2개의 전사물 변이체, G1T1 및 G1T2를 가지며, 이들은 단지 하나의 보유된 인트론 서열에 의해 서로 상이하다. 정렬자가 상기 생성된 리드를, 개시 부위 분포, 서열 편향, 길이 편향 및 상기 언급된 스플라이싱 부위 디뉴클레오티드 어노테이션(표 2)과 같은 소정의 또는 유래된 정보의 상이한 가중치 모델을 사용하는 프로그램화된 확률 연산을 사용하여 상기 G1 유전자좌내에 분배하는 경우, 최종적으로 할당된 리드를 카운트하고 예를 들어 지도화된 백만 단편당 엑손의 킬로염기당 단편(FPKM)으로 표준화하여 상대적인 전사물 농도에 대한 하나의 척도 및 G1T1과 G1T2간의 비를 획득한다. 상기 실험 설정에 따라, 상기 FPKM 값은 매우 동일한 실험내의 기술적 복제물로부터 계산되거나 또는 선행 참조 실험으로부터 추정된 신뢰도 구간을 함유한다. 정렬 연산이 거짓 편향을 부과하고 거짓 발현 값을 생성시키는 경우 상기 G1T1 및 G1T2에 대한 결과는 틀린 채로 있으며, 더욱이 상기 샘플 자체 또는 실험 조건이 변하는 경우 완전히 제멋대로 될 수 있다. 유사한 복잡성(예를 들어 유사한 길이, 근접시 인트론 유지)을 갖는 참조 세트, Ref1T1 및 Ref1T2의 실측 자료 지식만이 리드 할당까지의 서열분석을 통한 상기 라이브러리 생성으로부터의 특정한 실험의 수행성능을 평가하고 유사한 복잡성의 유전자 및 전사물 변이체 분포의 신뢰도 구간을 계산할 수 있게 한다. 따라서 상기 참조 리드 할당을 사용하여, 예를 들어 바람직하게는 FPKM 값에 대한 표준화를 근거로 상기 샘플 리드의 통계학적 리드 할당을 조절 또는 이동시킬 수 있다. 상기 참조 세트의 리드 할당의 오차를 상기 참조 세트의 공지된 조성 및 양(주어진 플랫폼에 적합한, 느긋하게 선택될 수 있는 소정의 값)에 기인하여 보정할 수 있으며 상기 보정을 상기 샘플 리드 할당의 변형에 적용할 수 있다.
한편으로, 본 발명의 상기 방법은 바람직하게는 NA 검출 또는 정량분석 방법, 바람직하게는 마이크로-어레이 분석 또는 qPCR을 하나 이상의 샘플상에서 수행함을 포함하며, 여기에서 적어도 하나의 탐침은 상기 참조 세트의 적어도 하나의 NA 분자에 결합하고 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 적어도 하나의 탐침으로부터 생성되는 신호에 근거한 측정 결과를 사용하여 상기 NA 검출 또는 정량분석 방법에서 탐침에 결합하는 하나 이상의 샘플의 전사물 변이체로부터 생성되는 신호에 근거한 측정 결과를 조절, 확인 또는 변형시킨다. 외부 대조군을 사용하여 측정 결과를 조절, 확인 또는 변형시키는 방법은 당해 분야에 공지되어 있다. 예를 들어 문헌[Devonshire et al., 2010]을 참조하시오.
본 발명의 과정에서, 놀랍게도 상기 방법의 적용이 NA 서열분석 방법의 평가에 특히 적합한 것으로 밝혀졌다. 상기는 또한 NA 서열분석 방법의 평가, 또는 NA 검출 또는 정량분석 방법의 평가에 매우 적합하다. 따라서, 본 발명의 또 다른 태양에서, NA 서열분석 방법을 평가하거나 또는 NA 검출 또는 정량분석 방법을 평가하기 위한 방법을 제공하며, 상기 방법은
a) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 상이한 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지는, 전사물 변이체(앞서 설명된 바와 같은)를 모방하는 인공 NA 분자의 참조 세트를 제공하고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하며,
여기에서 상기 NA 분자의 적어도 2개, 바람직하게 각각은 소정의 몰량으로 존재하는 단계;
b1) 상기 NA 서열분석 방법을 평가하기 위해서, 리드 생성 및 할당에 기반한 NA 서열분석을 수행하고, 여기에서 참조 리드 할당이 상기 참조 세트의 리드에 의해 생성되는 단계; 또는
b2) 상기 NA 검출 또는 정량분석 방법을 평가하기 위해서, 상기 참조 세트상에서 상기 NA 검출 또는 정량분석 방법을 수행하고,
여기에서 적어도 하나의 탐침이 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 단계;
c) 임의의 단계 b)의 산출 결과, 특히 산출 몰량, 산출 농도, 및/또는 상기 NA 서열분석 방법 평가의 경우에, 상기 참조 세트의 NA 분자 중 적어도 하나의 할당된 리드의 수, 및/또는 상기 참조 세트의 적어도 2개의 NA 분자의 적어도 하나의 비를 상기 소정의 몰량에 비교하고/하거나, 상기 NA 서열분석 방법 평가의 경우에, 할당된 리드의 수 및/또는 상기로부터 계산되거나 예상되는 비 및/또는 산출결과에 비교하는 단계
를 포함한다.
본질적으로, 본 발명은 다양한 NA 분석 방법들을 "벤치마크"(또는 비교 또는 평가)하여, 조사자들(또는 NA 분석 방법 및/또는 NA 분석 장비의 생산자)로 하여금 그들의 방법을, 특히 전사물 변이체(복잡한 유기체의 전사체에 전형적이므로)를 신뢰성 있게 식별 및/또는 정량분석할 수 있음에 관하여 최적화되게 하는 방법을 제공한다.
상기 참조 세트에 대해 공지된 매개변수들(예를 들어 농도, 존재하는 서열 등 - 즉, 상기 참조 세트는 이 경우에 공지된 대조군을 나타낸다)로부터, 숙련가는 예상되는 결과(예를 들어 리드의 수, 외삽된 농도 등)를 계산하거나 예측할 수 있다. 상기 (실제) 산출 결과를 상기 예상 결과와 비교함으로서, 숙련가는 실제 결과와 예상 결과간의 상이함을 측정하여 상기 핵산 서열분석 방법을 평가할 수 있다.
현저하게, 또한 핵산 서열분석 방법의 계산적 태양을, 상이한 계산 방법 부분(예를 들어 연산)을 평가하거나 또는 상기 방법 부분(예를 들어 연산 또는 연산들)을 개선시키기 위해서 (반복적으로) 상기 참조 세트의 종래의 서열분석 측정을 사용하고 (반복적으로) 상기 서열분석 방법의 계산적 부분을 변화시킴으로써 평가할 수 있다.
이롭게, 본 발명의 임의의 참조 세트(하기 참조)는 특히 상기 참조 세트의 NA 분자의 적어도 2개, 바람직하게는 각각이 소정의 몰량으로 존재할 때, 상기 본 발명의 상기 방법에 적합하다.
본 발명의 과정에서, 다수의 상이한 참조 세트(및 그의 제조 방법)를 특성화하고 최종적으로 앞서 언급한 방법들에 특별히 매우 적합한 참조 세트(및 그의 제조 방법)를 발견하였다. (그러나, 상기 앞서 언급한 방법들은 본 발명의 참조 세트를 사용함으로 제한되지 않으며; 다른 참조 세트들도 또한 적합할 수 있다(그러나 본 발명의 참조 세트보다 덜 그렇다)).
따라서, 본 발명의 또 다른 태양에서, 전사물 변이체를 모방하는 인공 NA 분자, 바람직하게는 RNA 또는 DNA 분자의 참조 세트의 생성 방법을 제공하며, 상기 방법은 하기를 포함한다:
A) 천연 진핵생물 유전자, 바람직하게는 동물 또는 식물 유전자, 보다 바람직하게는 척추동물 유전자, 훨씬 더 바람직하게는 포유동물 유전자, 특히 인간 유전자의 그룹으로부터 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 유전자를 선택한다. 이는 상기와 같은 유전자를 발견하는 분야에 공지되어 있다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다. 예를 들어, 상기(또는 다른 공개 데이터베이스에 사용하기 위한 그의 어노테이션된 서열 또는 그의 명칭)를 공개적으로 입수할 수 있는 데이터베이스, 예를 들어 Ensembl, 국립 생물공학 정보 센터(NCBI) GenBank 또는 다른 NCBI 데이터베이스로부터 획득할 수 있다. 예로서, 인간 유전자의 경우, 하기의 NCBI 검색 질의로부터 유전자를 선택할 수 있다: http://www.ncbi.nlm.nih.gov/gene/?term=Homo+sapiens[Orgn]
한편으로, 또는 추가로, Ensembl 데이터베이스(http://www.ensembl.org)에서 게놈을 훑어볼 수 있다.
바람직하게, 상기 유전자는 그의 전사물 변이체(전사물 표)에 관하여 충분히-어노테이션되어 있으며 인트론/엑손이 어노테이션되어 있다.
B) 각각의 선택된 유전자에 대해 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 천연 mRNA 전사물 변이체를 선택하고, 여기에서 각각의 전사물 변이체는 적어도 100 nt의 길이를 가지며 적어도 하나의 엑손을 포함한다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다. 예로서, 상기 Ensembl 데이터베이스는 유전자(예를 들어 인간 유전자)의 충분히-어노테이션된 전사물 변이체(또한 전사물 표라 칭한다)를 함유한다. 예를 들어 http://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000139618;r=13:32889611-32973805는 유전자 BRCA2의 전사물 표를 나타낸다. Ensembl은 또한 어노테이션된 스플라이싱 사건(ASE)을 함유한다(Wang et al., 2008; Koscielny et al., 2009). 상기 서열 어노테이션, 텍스트-기반 포맷으로서 FASTA 파일은 순수한 뉴클레오티드 서열을 나타내며, 전형적으로는 seqname - 염색체 또는 스캐폴드의 명칭과 같은 모든 관련된 정보를 함유하는 GTF 파일(일반적인 전송 포맷)에 통상적으로 유지되는 전사물 변이체 어노테이션과 함께 사용된다; 염색체 명칭을 'chr' 접두사와 함께 또는 상기 접두사 없이 제공할 수 있으며; 소스 - 상기 특징을 생성시킨 프로그램의 명칭, 또는 데이터 소스(데이터베이스 또는 프로젝트 명칭); 특징 - 특징 유형 명칭, 예를 들어 유전자, 변이, 유사성; 개시 - 1에서 시작하는 서열 넘버링을 갖는 상기 특징의 개시 위치; 종료 - 1에서 시작하는 서열 넘버링을 갖는 상기 특징의 종료 위치; 점수 - 이동 소수점 값; 가닥 - +(순방향) 또는 -(역방향)으로서 정의된다; 프레임 - '0', '1' 또는 '2' 중 하나. '0'은 상기 특징의 첫 번째 염기가 코돈의 첫 번째 염기임을 가리키고, '1'은 두 번째 염기가 코돈의 첫 번째 염기임을 가리키는 등이다; 속성 - 태그-값 쌍의 세미콜론-분리된 목록, 각각의 특징에 관한 추가적인 정보를 제공한다. 상기 GTF 파일로부터, 상이한 전사물들을 시각 검사를 위해 줌 기능을 갖는 프로그램에 의해 나타낼 수 있다.
C) 적어도 하나의 엑손을 포함하는 상기 선택된 천연 mRNA 전사물 변이체 각각의 서열을 제공하고, 임의로 여기에서 상기 서열은 또 다른 NA 유형, 예를 들어 DNA 서열로 전환된다. RNA를 DNA 서열로 전환시키는 것은 사소한 일이다. 바람직하게는 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다. 이롭게, 상기 mRNA 전사물 변이체는 성숙한 전사물이다.
D) 단계 C)의 각 서열을 하기에 의해 변형시킨다:
각 서열의 각 엑손의 서열을 각각의 엑손에 대해 독립적으로, 대략 동일한 길이의 서열(엑손 서열로서)로 치환시키고,
여기에서 상기 대략 동일한 길이의 서열을 하기의 그룹 중에서 선택하며:
바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 임의의 다른 역전된 천연 서열(역전은 정렬 소프트웨어가 상기 서열의 그의 원래 유전자좌로의 정렬을 방지하고 또한 그의 원래 보체와의 하이브리드화를 방지한다), 비-천연 랜덤 서열, 및 이들의 조합, 바람직하게 상기 대략 동일한 길이의 서열을 하기의 그룹 중에서 선택하고:
바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 비-천연 랜덤 서열, 및 이들의 조합, 보다 바람직하게는 상기 대략 동일한 길이의 서열을 하기의 그룹 중에서 선택하고:
바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 및 이들의 조합,
바람직하게 여기에서 상기 대략 동일한 길이의 서열을 3개 이하, 바람직하게는 2개 이하, 특히 1개 이하의 디뉴클레오티드를 서로 독립적으로 임의의 다른 디뉴클레오티드에 의해, 바람직하게는 GT, GC 또는 AT에 의해 치환시키고/시키거나 3개 이하, 바람직하게는 2개 이하, 특히 1개 이하의 디뉴클레오티드를 서로 독립적으로 임의의 다른 디뉴클레오티드에 의해, 바람직하게는 AG, AC 또는 AT에 의해 치환시킴으로써 변형시키나, 단 바람직하게는 상기 디뉴클레오티드 교환을, 엑손-암호화된 인트론 접합 디뉴클레오티드의 풍부성이 예를 들어 엑손 좌표에 대한 정보(ICE) 데이터베이스(Chong et al., 2004)(하나의 서열에서 엑손인 것은 상기 다른 전사물 중에 존재하지 않음으로써 또 다른 전사물에 대한 인트론일 수 있다)에 제공된 바와 같은 천연 빈도를 반영하기 위해 90 내지 100%(GT-AG), 0 내지 10%(GC-AC) 및 0 내지 2%(AT-AT)이도록 수행해야 한다.
상기에 의해 인공 전사물 서열(적어도 하나의 인공 엑손을 포함한다)의 세트를 획득하나, 단
상기 동일한 선택된 유전자의 상기 선택된 천연 mRNA 전사물 변이체의 서열로부터 획득된 인공 전사물 서열은 적어도 80 nt 길이의 서열(바람직하게는 단일 엑손 서열 중에 포함된다)을 공유해야 하며, 바람직하게는 단,
단계 C)의 서열의 엑손 서열이 단계 C)의 서열의 또 다른 엑손 서열에 일치하는 경우, 상기 엑손 서열 및 상기 또 다른 엑손 서열은 대략 동일한 길이의 동일한 상기 서열에 의해 치환되어야 한다.
바람직하게, 상기 방법 단계를 소프트웨어에 의해 컴퓨터-실행한다. 상기 단계(및 모든 후속의 바람직한 컴퓨터 단계)를 예를 들어 널리 사용되는 소프트웨어 CLC 메인 워크벤치(Main Workbench)(퀴아겐(QIAGEN)), 바이오컨덕터(Bioconductor) 패키지, UCSC 게놈 브라우저, 또는 다른 것들로 수행할 수 있다.
서열들을 또한, 특히 바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 임의의 다른 역전된 천연 서열, 또는 비-천연 랜덤 서열이 전체 엑손을 채우기에 너무 짧은 경우, 대략 동일한 길이의 서열을 형성하도록 합할 수도 있다.
이롭게, 바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 또는 임의의 다른 역전된 천연 서열 또는 비-천연 랜덤 서열의 길이는, 특히 너무 많은 짧은 서열 신장부들이 결합하여 불리하게 진핵생물 서열에 너무 상동성인 서열을 생성시키는 것을 피하기 위해서, 적어도 10 nt, 바람직하게는 적어도 20 nt, 보다 바람직하게는 적어도 50 nt, 특히 적어도 100 nt이다.
이롭게, 몇몇 제한 부위들을 단일의 점 돌연변이를 도입시킴으로써(예를 들어 XhoI 및 NsiI의 제한 부위를 제거함으로써) 상기 인공 전사물 서열로부터 제거하여 클로닝시 보다 양호한 처리를 허용한다.
E) 단계 D)의 세트의 인공 전사물 서열 중 적어도 하나를 임의로 중복시키고 상기 중복된 서열을 상기 세트에 가하여, 단계 F) 내지 K) 중 하나 이상에서 선택적 변형을 위한 사본을 포함하는 세트를 획득한다.
상기 중복은 상기 참조 세트 중에 존재해야 하는 전사물 변화 사건의 모방을 허용하지만(상기 참조 세트가 보다 적합할 수록 선택적 전사 사건이 보다 광범위하게 획득된다), 상기 선택된 유전자와 함께 발생하지는 않는다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다.
F) 적어도 하나의 서열을 상기 세트의 인공 전사물 서열의 적어도 하나에 임의로 삽입하고,
여기에서 상기 적어도 하나의 삽입된 서열은 각각 서로 독립적으로 단계 D)의 인공 전사물 서열 중 어느 하나의 동일한 길이의 센스 또는 안티-센스 서열(즉 역 보체 서열)에 일치하며 바람직하게는 5 nt 내지 10000 nt, 특히 10 nt 내지 1000 nt의 길이를 갖는다.
이롭게, 인공 전사물 서열당 5개 이하, 바람직하게는 4개 이하, 보다 바람직하게는 3개 이하, 특히 2개 이하의 삽입이 수행된다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다.
G) 상기 세트의 인공 전사물 서열 중 적어도 하나로부터 1 nt 내지 10000 nt 범위의 길이를 갖는 적어도 하나의 서열을 임의로 제거하고,
여기에서 상기 하나 이상의 인공 전사물 서열은 각각 적어도 100 nt의 크기로 남아있으며 적어도 하나의 엑손 서열을 포함하는 상태로 남아있는다.
이롭게, 인공 전사물 서열당 5개 이하, 바람직하게는 4개 이하, 보다 바람직하게는 3개 이하, 특히 2개 이하의 제거를 수행한다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다.
단계 E 내지 G의 조합에 의해, 상기 선택된 천연 mRNA 전사물 중에 존재하지 않는 추가적인 선택적 전사 사건을 포함시키는 것이 가능하다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다.
H) 상기 인공 전사물 서열 각각의 첫 번째 뉴클레오티드로서 구아노신을, 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써, 첫 번째 염기를 구아노신으로 교환함으로써 또는 구아노신을 5' 단부에 가함으로써, 바람직하게는 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써, 또는 첫 번째 염기를 구아노신으로 교환함으로써, 특히 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써 임의로 확립시킨다.
상기 첫 번째 염기가 구아노신인 것은 T7 폴리머라제에 의한 효율적인 전사를 허용한다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다.
I) 상기 인공 전사물 서열의 세트가 GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖도록 상기 세트의 인공 전사물 서열 중 적어도 하나를 임의로 변형시킨다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다. 이는 상기 생성된 참조 세트를 WO 2011/095501 A1에 개시된 복잡성 감소 방법에 양립성이고 특히 적합하게 한다.
본 명세서에서, 전체 발명과 관련해서뿐만 아니라, "필수적으로 랜덤하게 분포된 존재"(본 발명의 목적을 위해서)(상기는 "필수적으로 균일하게 분포된 존재"일 수도 있다)를 가짐은 - 광범위하게 사용되는 카이-제곱 검정(피어슨(Pearson)에 의해 개발된 바와 같은)을 적합한 분포로서 별개의 균일한 분포(즉 모든 사건이 균등하게 있음직함)를 갖는 상기 존재에 적용할 때 - 상기 생성되는 p 값(전형적으로 상기 카이-제곱 값에 대해 표로 작성된다)이 0.1 초과, 바람직하게는 0.2 초과, 보다 바람직하게는 0.3 초과, 훨씬 더 바람직하게는 0.5 초과, 특히 0.8 초과임을 의미한다. 상기 카이-제곱 검정을 적용하는 방법은 당해 분야에 주지되어 있다. 상기 카이-제곱 검정을 적용하는 방법에 대해서 또한 실시예 4를 참조하시오.
J) 바람직하게는 폴리-A 꼬리 서열을, 바람직하게는 적어도 10개, 특히 적어도 20개 아데노신으로 이루어지는 상기 세트의 인공 전사물 서열 중 하나 이상, 바람직하게는 전부에 가한다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행한다. 임의로, 상기 폴리-A 꼬리 뒤에 지표 서열(DNA 바코드 또는 서열 표지)을 상기 세트의 인공 전사물 서열 중 하나 이상, 바람직하게는 전부에 가한다. 상기 지표 서열은 상기 참조 세트의 제조 중 선택적인 정량분석 및 확인 방법을 가능하게 하지만, 참조 서열로서 적용 중에는 가릴 필요가 있다. 상기 가림은, 상기 지표 서열을, 특정한 이어지는 작업흐름(폴리-A 프라이밍을 포함한 RNA 서열분석 프로토콜)에 의해 보이지 않거나 또는 상기 지표 서열을 임의의 잠재적인 리드 및 참조 어노테이션에서 감추어야 하는 상기 폴리-꼬리 밖에 놓음으로써 성취될 수 있다. 바람직하게, 상기 방법 단계를 소프트웨어로 컴퓨터-실행하거나,
K) 또는 바람직하게는 단계 E 내지 J 중 적어도 2개의 임의의 조합으로 수행하며, 바람직하게는 여기에서 각각의 방법 단계를 단지 1회만 수행하고;
L) 상기 세트의 각각의 인공 전사물 서열의 경우:
상기 전체 인공 전사물 서열을 포함하는 NA 분자를 물리적으로 합성한다. NA, 특히 DNA 및 RNA 분자를 합성하는 방법은 당해 분야에 공지되어 있다. DNA 및 RNA를 생체내(재조합 세포, 예를 들어 이 콜라이에서 발현된다) 또는 시험관내 생화학적 방법(예를 들어 DNA/RNA 폴리머라제에 의한 합성/증폭, 예를 들어 폴리머라제 쇄 반응 - PCR)에 의해 생성시킬 수 있을 뿐만 아니라 화학적으로 합성시킬 수 있다. 상기 인공 NA가 DNA인 경우, 상기를 바람직하게는 드노보 DNA 합성에 의해 합성하고 PCR에 의해 증폭시킨다. 플라스미드내로의 클로닝에 의한 생체내 증폭, 미생물내로의 형질전환, 서열-확인 및 상기 형질전환된 미생물의 성장이 또한 가능하다. 상기 DNA 주형으로부터, RNA를 T7 RNA 폴리머라제에 의한 전사에 의해 합성할 수 있다. 바람직하게, 상기 NA가 RNA인 경우, 상기를 특히 T7 RNA 폴리머라제에 의해 DNA로부터 전사시킨다.
M) 바람직하게, 단계 L)의 NA 분자가 RNA 분자인 경우, 5' 캡-구조를 상기 RNA 분자에 물리적으로 가한다. 이는 실제 진핵생물 전사물의 훨씬 더 가까운 모방을 성취한다. mRNA의 캡핑을 효소적으로, 예를 들어 우두 캡핑 시스템(뉴잉글랜드 바이오랩스 인코포레이티드(New England BioLabs, Inc.)에 의해 수행할 수 있다. 또한 WO 2009/058911 A2를 참조하시오;
상기에 의해, 바람직하게는 RNA 또는 DNA 분자의 참조 세트인 전사물 변이체를 모방하는 인공 NA 분자의 참조 세트를 물리적으로 획득한다.
바람직한 실시태양에서, 단계 D) 내지 G), 바람직하게는 모든 단계를, 상기 인공 NA 분자의 참조 세트가 진핵생물 유전자, 바람직하게는 동물 또는 식물 유전자, 보다 바람직하게는 척추동물 유전자, 훨씬 더 바람직하게는 포유동물 유전자, 특히 인간 유전자에 대해 자연에서 발생하는 선택적 전사 사건을 모방하는 한 수행하며, 상기 사건은 바람직하게는 하기의 그룹 중에서 선택된다:
선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된(skipped) 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들.
또 다른 바람직한 실시태양에서, 인공 NA 분자의 참조 세트는 하기의 그룹 중에서 선택된 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개, 특히 모든 선택적인 전사 사건들을 모방한다:
선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들.
또 다른 바람직한 실시태양에서, 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 개시 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%는 GT이며, 여기에서 상기 인트론 개시 디뉴클레오티드는 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기는 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나(바람직하게는 나타내고), 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 종료 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%는 AT이며, 여기에서 상기 인트론 종료 디뉴클레오티드는 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기는 상기 또 다른 인공 NA 분자에 대한 인트론을 나타낸다.
또 다른 바람직한 실시태양에서, 상기 인공 NA 분자의 참조 세트는 500 nt 내지 2000 nt, 바람직하게는 750 nt 내지 1500 nt, 특히 1000 nt 내지 1400 nt의 평균 서열 길이를 가지며; 바람직하게는 300 nt 내지 1200 nt, 바람직하게는 600 nt 내지 900 nt, 특히 700 nt 내지 800 nt의 표준 편차; 적어도 100 nt의 최소 크기; 및 바람직하게는 10000 nt의 최대 크기를 갖는다.
또 다른 바람직한 실시태양에서, 상기 인공 NA 분자의 참조 세트는 GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖는다. 이는 상기 생성된 참조 세트를 WO 2011/095501 A1에 개시된 복잡성 감소 방법에 특히 적합하게 한다.
또 다른 바람직한 실시태양에서, 상기 참조 세트의 적어도 50%, 바람직하게는 전체 인공 NA 분자는 25% 내지 55%의 평균 GC 함량을 갖는다. 바람직하게, 상기 평균 GC 함량은 천연 유전자가 선택되는 종들(또는 계통발생 그룹)의 전사물의 평균 GC 함량과 동일하도록 선택된다.
또 다른 바람직한 실시태양에서, 상기 참조 세트의 각각의 인공 NA 분자는 5' 개시 뉴클레오티드로서 구아노신을 갖는다.
또 다른 바람직한 실시태양에서, 상기 참조 세트의 인공 NA 분자 중 적어도 하나, 바람직하게는 각각은 상기가 RNA 분자인 경우 5'-캡 구조를 갖는다.
또 다른 바람직한 실시태양에서, 상기 방법은 상기 인공 NA 분자의 참조 세트를 제공함을 또한 포함하며, 여기에서 상기 참조 세트의 NA 분자 중 적어도 2개, 바람직하게 각각은, 바람직하게는 동일한 용기 중에 소정의 몰량으로 존재한다. 이롭게, 상기는 사용 준비가 된 키트의 형태로 제공된다. 바람직하게, 상기 NA 분자의 적어도 2개의 각각의 몰량은 적어도 102, 바람직하게는 적어도 103, 보다 바람직하게는 적어도 105, 특히 적어도 106배만큼 상이하며, 특히 상기 NA 분자 중 적어도 2개는 액체 중에 용해된 상태로 제공되거나 또는 액체 중에 용해되거나 희석될 준비가 되어 있으며, 여기에서 그들 각각의 농도 또는 최종 농도 범위는 0.01 아토몰/㎕ 내지 100 펨토몰/㎕, 또는 100 젭토몰/㎕ 내지 1 펨토몰/㎕이다.
상기에 논의된 바와 같이, 안정화 및 처리 오차의 감소가 중요하다. 따라서, 매우 바람직한 실시태양에서, 본 발명의 방법은 바람직하게는 안정화제와 함께, 바람직하게는 용기 중에서 물리적으로 획득된 참조 세트를 건조, 바람직하게는 동결-건조시키는 단계를 포함한다.
또 다른 바람직한 실시태양에서, 인공 NA 분자의 참조 세트의 서열은 NCBI GenBank 데이터베이스 수납 번호가 표 3에 나열된(즉 대부분의 공지된 진핵생물 서열과 유사성을 갖지 않는다), 바람직하게는 표 3 및 표 4 중 어느 하나에 나열된(즉 대부분의 공지된 진핵생물 및 대부분의 공지된 원핵생물/바이러스 서열 모두와 유사성을 갖지 않는다) 서열, 특히 2014년 6월 15일자 발매 NCBI GenBank 데이터베이스 202의 모든 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)는 10-1 미만, 바람직하게는 1 미만, 특히 10 미만이다. 상기 유사성을 하기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정한다: 낮은 복잡성 영역의 필터링, 1차 갭 비용(linear gap cost) 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기. 상기 통계학적 유의수준 한계의 설명에 대해서 문헌[Karlin & Altschul, 1990], 및 GenBank에의 도입에 대해서 문헌[Benson et al., 2013]을 참조하시오. 상기 실시태양은 복잡한 샘플에 첨가시에조차 상기 참조 세트의 서열의 명확한 식별을 허용하기 때문에(상기가 예를 들어 RNA-seq에 의해 쉽게 성취될 수 있는, 예를 들어 30 nt의 최소 길이를 갖는 한) 본 발명의 문제를 해결하기에 뜻밖에도 대단히-적합하다. 현행 GenBank 버전을 ftp://ftp.ncbi.nlm.nih.gov/genbank/ 하에서 다운로드용으로 자유롭게 입수할 수 있으며, BLAST 소프트웨어는 ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/ 하에서 다운로드용으로 자유롭게 입수할 수 있다. 사용이 용이한 GenBank의 BLAST 검색이 또한 http://blast.ncbi.nlm.nih.gov/Blast.cgi (뉴클레오티드 블라스트, 선택된 데이터베이스 뉴클레오티드 수집(nr/nt), 매우 유사한 서열(메가블라스트))상에서 가능하다.
본 발명은 또한 본 발명의 상기 방법의 임의의 실시태양에 의해(특히 본 명세서에서 명백히 언급된 실시태양에 의해) 획득될 수 있는, 전사물 변이체를 모방하는 인공 NA 분자의 참조 세트를 제공한다.
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본 발명의 또 다른 태양에서, NA 분자 중 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개 그룹을 포함하는 전사물 변이체, 바람직하게는 RNA 분자 또는 DNA 분자, 특히 RNA 분자를 모방하는 인공 NA 분자의 참조 세트를 제공하며, 이때 각 그룹은 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지고,
여기에서 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하다.
본 발명의 과정에서, 인공 NA 분자의 참조 세트는 본 발명의 목적에 특별히 적합한 것으로 밝혀졌다. 상기 분자를 SIRV(스파이크-인 RNA 변이체)라 칭하며 본 발명의 경우 서열번호 1 내지 148(실시예 1 참조)에 개시되어 있다. 따라서, 또 다른 태양에서, 본 발명은 서열번호 1 내지 148의 그룹 중에서 선택된 전체 서열과 적어도 50%, 바람직하게는 적어도 60%, 보다 바람직하게는 적어도 70%, 훨씬 더 바람직하게는 적어도 80%, 더욱 훨씬 더 바람직하게는 적어도 90% 또는 적어도 95%, 특히 100% 일치하는 서열을 포함하는 NA 분자, 바람직하게는 DNA 분자 또는 RNA 분자를 제공한다. 상기 서열의 큰 변화는 상기 서열이 오직 NA 분석 방법에서 참조 서열로서 사용하기 위한 것인 한 생물학적 기능이 보존될 필요가 없기 때문에 가능하다. 바람직하게 상기 서열번호에 대한 변화는 상기에서 말한 바와 같이 표 3의 서열에 유사성을 갖지 않는다. 이러한 변화는 상술한 방법에 의해 획득될 수 있다.
상기 SIRV의 엑손은 자체가 또 다른 서열에 포함되는 경우에 조차 본 발명의 목적에 매우 적합하기 때문에, 본 발명은 또한 서열번호 156 내지 334의 그룹 중에서 선택된 전체 서열과 적어도 50%, 바람직하게는 적어도 60%, 보다 바람직하게는 적어도 70%, 훨씬 더 바람직하게는 적어도 80%, 더욱 훨씬 더 바람직하게는 적어도 90% 또는 적어도 95%, 특히 100% 일치하는 서열을 갖는 적어도 하나의 엑손을 갖는 서열을 포함하는 NA 분자, 바람직하게는 DNA 분자 또는 RNA 분자를 제공한다.
또한, 상기 SIRV의 단편은 또 다른 NA 분자에 포함될 때 본 발명의 목적에 유용하다. 따라서 본 발명은 또한 적어도 80개, 바람직하게는 적어도 150개, 바람직하게는 적어도 200개, 보다 바람직하게는 적어도 300개, 특히 적어도 400개의 연속적인 뉴클레오티드의 서열을 포함하는 NA 분자, 바람직하게는 DNA 분자 또는 RNA 분자를 제공하며, 상기 서열은 서열 단편과 적어도 50%, 바람직하게는 적어도 60%, 보다 바람직하게는 적어도 70%, 훨씬 더 바람직하게는 적어도 80%, 더욱 훨씬 더 바람직하게는 적어도 90% 또는 적어도 95%, 특히 100% 일치하고, 서열번호 1 내지 148의 그룹 중에서 선택된 서열의 적어도 80 nt, 바람직하게는 적어도 150 nt, 바람직하게는 적어도 200 nt, 보다 바람직하게는 적어도 300 nt, 특히 적어도 400 nt의 최소 크기를 갖는다.
바람직한 실시태양에서, 본 발명의 NA 분자는 NA 분자 중 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개 그룹을 포함하는, 전사물 변이체를 모방하는 인공적인 NA 분자의 참조 세트로서 제공되며, 이때 각 그룹은 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 본 발명의 NA 분자로 이루어지고, 여기에서 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이며, 여기에서 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하다.
바람직하게, 본 발명의 임의의 참조 세트는 하기의 그룹 중에서 선택된 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개, 특히 모든 선택적인 전사 사건을 모방한다:
선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들.
본 발명의 임의의 참조 세트의 또 다른 바람직한 실시태양에서, 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 개시 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%는 GT이며, 여기에서 상기 인트론 개시 디뉴클레오티드는 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기는 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나(바람직하게는 나타내고), 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 종료 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%는 AT이며, 여기에서 상기 인트론 종료 디뉴클레오티드는 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기는 상기 또 다른 인공 NA 분자에 대한 인트론을 나타낸다.
또 다른 바람직한 실시태양에서, 본 발명의 임의의 참조 세트는 500 nt 내지 2000 nt, 바람직하게는 750 nt 내지 1500 nt, 특히 1000 nt 내지 1400 nt의 평균 서열 길이를 가지며; 바람직하게는 300 nt 내지 1200 nt, 바람직하게는 600 nt 내지 900 nt, 특히 700 nt 내지 800 nt의 표준 편차; 적어도 100 nt의 최소 크기; 및 바람직하게는 10000 nt의 최대 크기를 갖는다.
또 다른 바람직한 실시태양에서, 본 발명의 임의의 참조 세트는 25% 내지 55%의 평균 GC 함량을 갖는다.
또 다른 바람직한 실시태양에서, 본 발명의 임의의 참조 세트는 GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖는다.
또 다른 바람직한 실시태양에서, 본 발명의 임의의 참조 세트의 각각의 인공 NA 분자는 5' 개시 뉴클레오티드로서 구아노신을 갖는다.
또 다른 바람직한 실시태양에서, 본 발명의 임의의 참조 세트의 인공 NA 분자 중 적어도 하나, 바람직하게는 각각은, 상기가 RNA 분자인 경우, 5'-캡 구조를 갖고/갖거나 적어도 10, 바람직하게는 적어도 20, 특히 적어도 30개 아데노신의 폴리-A 꼬리를 갖는다. 바람직하게, 본 발명의 임의의 참조 세트의 서열은 NCBI GenBank 데이터베이스 수납 번호가 표 3에 나열된, 바람직하게는 표 3 및 표 4 중 어느 하나에 나열된 서열, 특히 2014년 6월 15일자 발매 NCBI GenBank 데이터베이스 202의 모든 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)는 10-1 미만, 바람직하게는 1 미만, 특히 10 미만이고, 여기에서 상기 유사성을 하기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정한다: 낮은 복잡성 영역의 필터링, 1차 갭 비용 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기.
특히 바람직한 실시태양에서, 본 발명의 인공 NA 분자의 임의의 참조 세트를 제공하며, 여기에서 상기 NA 분자 중 적어도 2개, 바람직하게 각각은, 바람직하게는 동일한 용기 중에 소정의 몰량으로 존재하고; 바람직하게, 상기 NA 분자의 적어도 2개의 각각의 몰량은 적어도 102, 바람직하게는 적어도 103, 보다 바람직하게는 적어도 105, 특히 적어도 106배만큼 상이하며, 특히 상기 NA 분자 중 적어도 2개는 액체 중에 용해된 상태로 제공되거나 또는 액체 중에 용해되거나 희석될 준비가 되어 있으며, 여기에서 그들 각각의 농도 또는 최종 농도 범위는 0.01 아토몰/㎕ 내지 100 펨토몰/㎕, 또는 100 젭토몰/㎕ 내지 1 펨토몰/㎕이다. 광범위한 농도를 갖는 것은, 예를 들어 높은 동적 범위의 검출을 갖는 장비 및 방법을 개발하는 것이 보다 큰 도전이기 때문에, 장비 및 방법(예를 들어 RNA-seq에서) 평가에 보다 양호하다.
상기에 논의된 바와 같이, 처리 오차의 안정화 및 감소는 중요하다. 상응하게, 또 다른, 특히 바람직한 실시태양에서, 본 발명의 인공 NA 분자의 참조 세트를 용기 중에서, 바람직하게는 안정화제와 함께 건조, 바람직하게는 동결-건조시켜 제공한다.
DNA 서열을 RNA 서열로 전환시키거나(뉴클레오티드의 교환: T->U) 이와 역(뉴클레오티드의 교환: U->T)도 가능하다. 따라서, 하나의 서열을 본 명세서(서열 목록 포함) 중의 DNA 서열로서 제공하는 경우는 언제든 상기를 또한 그의 각각의 RNA 서열 및 이와 역으로서 판독할 것이다. 본 명세서에 사용되는 바와 같이, RNA는 전형적으로 단일-가닥인 반면 DNA 분자는 전형적으로 이중 가닥이다. 그러나, 또한 이중-가닥/단일-가닥 형태의 각각의 RNA/DNA는 본 발명에 청구될 뿐만 아니라 상기 청구된 서열에 상보성인 서열(예를 들어 cDNA)도 청구될 것이다.
적어도 하나 이상, 예를 들어 전체 NA 분자의 길이는 예를 들어 100 내지 1000000 뉴클레오티드, 바람직하게는 130 내지 100000 뉴클레오티드 또는 150 내지 10000 뉴클레오티드일 수 있다.
바람직한 실시태양에서, 상기 천연 또는 인공 유전자는 단백질(예를 들어 mRNA)을 암호화할 뿐만 아니라, 비 단백질-암호화 전사물, 예를 들어 microRNA, snoRNA 또는 rRNA를 포함한 조절 또는 촉매적 RNA뿐만 아니라 그들의 전구체, 특히 전-microRNA 또는 전-rRNA가 고려된다.
본 명세서에 사용되는 바와 같이, "유전자"는 하나 이상의 전사물을 형성하도록 전사되는 서열을 갖는 유전학적 뉴클레오티드에 관한 것이다.
본 명세서에 사용되는 바와 같이, "동형" 또는 "전사물 변이체"는 전사물의 특정 변이체에 관하여 사용된다.
본 명세서에 사용되는 바와 같은 "약"은 동일한 값 또는 주어진 값의 +/-10% 만큼 상이한 값을 지칭할 수 있다.
본 명세서에 사용되는 바와 같은 "포함하다"는 개방된 정의로서 이해될 것이며, 함유하는에서와 같이 추가의 구성원을 허용한다. 다른 한편으로 "이루어지는"은 상기 이루어지는 정의 특징의 추가의 요소 없이 폐쇄된 정의로서 간주된다. 따라서 "포함하다"가 보다 넓은 정의이며 "이루어지는" 정의를 함유한다. "포함하는"이란 용어를 사용하는 본 명세서의 임의의 정의는 또한 본 발명의 특별한 실시태양에서 이루어지는의 제한으로 판독될 수 있다.
상기 핵산 서열분석 단계를 당해 분야에 공지된 임의의 방법, 예를 들어 PCR 서열분석에 의해 수행할 수 있다. 상기와 같은 방법은 막삼-길버트(Maxam-Gilbert) 서열분석, 쇄-종결 방법, 샷건 서열분석, 브리지 PCR, 초병렬 서명 서열분석(MPSS), 폴로니 서열분석, 피로서열분석, 일루미나(Illumina)(솔렉사(Solexa)) 서열분석, SOLiD 서열분석, 이온 반도체 서열분석, DNA 나노볼 서열분석, 헬리스코프 단일 분자 서열분석, 단일 분자 실시간(SMRT) 서열분석, 나노포어 DNA 서열분석, 하이브리드화에 의한 서열분석, 질량 분광분석에 의한 서열분석, 미세유동성 상거 서열분석, 현미경-기반 기법, RNAP 서열분석, 시험관내 바이러스 대량고속처리 서열분석을 포함한다.
본 명세서에 사용되는 바와 같이, "자릿수"는 "십진법 자릿수"를 의미한다, 예를 들어 "6 자릿수"(또한 본 명세서에서 "6 자릿수"라 칭한다)에 걸쳐 있는은 1 내지 1x106 또는 2x10-7 내지 0.2에 걸쳐 있는 값을 의미한다.
임의의 본 발명의 방법 또는 단계를 명백히 제외하는 경우를 제외하고 컴퓨터-실행된 방법으로서 수행할 수 있다. 심지어 대개 NA 분자의 습식-화학 서열분석 및 합성 단계는, 예를 들어 자동 또는 반-자동 서열 판독기로부터 데이터를 조절 및 획득하기 위해서 컴퓨터에 의해 지원될 수 있다. 상기 컴퓨터 프로그램 생성물 또는 기억 장치에 또한, 샘플로부터 짧은 리드를 획득하는 리드 생성 성분, 예를 들어 서열분석기, 바람직하게는 컴퓨터 성분을 포함하는 서열분석기가 제공될 수 있다. 예를 들어, 컴퓨터 판독성 매체는 비제한적으로 자기 저장 장치(예를 들어 하드 디스크, 플로피 디스크, 자기 스트립...), 광학 디스크(예를 들어 콤팩트 디스크(CD), 디지털 만능 디스크(DVD)...), 스마트 카드, 및 플래시 메모리 장치(예를 들어 카드, 스틱, 키 드라이브...)를 포함할 수 있다.
참조 뉴클레오티드 서열에 관하여 "서열 일치성 퍼센트(%)"는, 서열 및 필요한 경우 도입되는 갭을 최대의 서열 일치성 퍼센트를 성취하기 위해 정렬시킨 후에, 상기 서열 일치성의 부분으로서 어떠한 보존적 치환도 고려하지 않은, 상기 참조 서열 중의 뉴클레오티드와 일치하는 후보 서열 중의 뉴클레오티드의 백분율로서 정의된다. 갭은 일치성의 결여를 일으킨다. 뉴클레오티드 서열 일치성 퍼센트의 측정을 위한 정렬을 당해 분야의 기술내에 있는 다양한 방식들, 예를 들어 공개적으로 입수할 수 있는 컴퓨터 소프트웨어, 예를 들어 BLAST, BLAST-2, ALIGN, ALIGN-2, Megalign(DNASTAR) 또는 EMBOSS 소프트웨어 패키지의 "니들" 짝 서열 정렬 어플리케이션으로 성취할 수 있다. 당해 분야의 숙련가들은 비교되는 서열들의 전체 길이에 걸쳐 최대의 정렬을 성취하는데 필요한 임의의 연산을 포함한, 서열 정렬에 적합한 매개변수들을 측정할 수 있다. 그러나, 본 명세서의 목적을 위해서, 뉴클레오티드 서열 일치성% 값을 EMBOSS 소프트웨어 패키지(유럽 분자 생물학 실험실로부터 공개적으로 입수할 수 있다; Rice et al., EMBOSS: the European Molecular Biology Open Software Suite, Trends Genet. 2000 Jun;16(6):276-7, PMID: 10827456)의 컴퓨터 프로그램 "니들"의 서열 정렬을 사용하여 계산한다.
상기 니들 프로그램을 웹 사이트 http://www.ebi.ac.uk/Tools/psa/emboss_needle/nucleotide.html 하에서 접근하거나 또는 http://emboss.sourceforge.net/로부터 EMBOSS 패키지의 부분으로서 로컬 설치를 위해 다운로드할 수 있다. 상기는 다수의 광범위하게 사용되는 UNIX 운영 체계, 예를 들어 리눅스(Linux)상에서 실행된다.
2개의 뉴클레오티드 서열을 정렬하기 위해서, 상기 니들 프로그램을 바람직하게는 하기의 매개변수들로 실행한다:
명령행: needle -auto -stdout -asequence SEQUENCE_FILE_A -bsequence SEQUENCE_FILE_B -datafile EDNAFULL -gapopen 10.0 -gapextend 0.5 -endopen 10.0 -endextend 0.5 -aformat3 pair -snucleotide1 -snucleotide2 (Align_format: pair Report_file: stdout)
주어진 뉴클레오티드 서열 B에, 상기 서열과, 또는 상기 서열에 대한 주어진 뉴클레오티드 서열 A(한편으로 주어진 뉴클레오티드 서열 B에, 상기 서열과, 또는 상기 서열에 대한 일정한 뉴클레오티드 서열 일치성%를 갖거나 포함하는 주어진 뉴클레오티드 서열 A로서 표현될 수 있다)의 뉴클레오티드 서열 일치성%를 하기와 같이 계산한다:
100 x 분수 X/Y
여기에서 X는 A 및 B의 상기 프로그램의 정렬에서 서열 정렬 프로그램 니들에 의해 일치하는 매치로서 기록된 뉴클레오티드의 수이고, Y는 B 중의 뉴클레오티드의 총수이다. 뉴클레오티드 서열 A의 길이가 뉴클레오티드 서열 B의 길이와 동일하지 않은 경우, A 대 B의 뉴클레오티드 서열 일치성%는 B 대 A의 뉴클레오티드 서열 일치성%와 동등하지 않음을 알 것이다. "A의 서열이 B의 전체 서열에 적어도 N% 일치하는" 경우, Y는 B의 전체 길이이다. 달리 구체적으로 진술되지 않는 한, 본 명세서에 사용된 모든 뉴클레오티드 서열 일치성% 값을 상기 니들 컴퓨터 프로그램을 사용하여 바로 앞 단락에 기재된 바와 같이 획득한다.
"서열 유사성", "서열 일치성", "서열을 공유하는" 및 유사한 용어들을 또한 서열의 역 보체에 적용할 것이다, 즉 "서열 A가 서열 B에 80% 일치한다"란 표현은 또한 "서열 A가 서열 B의 역보체(또는 안티센스 서열)에 80% 일치하는" 경우 참일 것이다.
본 명세서에서, NA 서열과 관련하여 "삽입"이란 용어는 또한 5' 또는 3' 단부에서의 직접적인 삽입(즉 5' 또는 3' 단부에서 첨가)을 의미할 수 있다.
예시적인 실시태양
본 발명의 방법의 특히 바람직한 실시태양은
a) 적어도 3개의 상이한 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 3개의 상이한 NA 분자로 이루어지는, 전사물 변이체를 모방하는 인공 NA 분자의 참조 세트를 제공하고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자는 동일한 인공 유전자의 참조 전사물 변이체이고,
여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자는 적어도 80 뉴클레오티드(nt) 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 특히 적어도 200 nt의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자는 적어도 80 nt 길이, 바람직하게는 적어도 100 nt, 보다 바람직하게는 적어도 150 nt, 훨씬 더 바람직하게는 적어도 200 nt, 특히 적어도 300 nt의 적어도 또 다른 서열만큼 상이하며,
여기에서 상기 인공 NA 분자는 각각 소정의 몰량으로 존재하고; 추가로
상기 인공 NA 분자는 각각
- 적어도 100 nt의 길이를 갖고 적어도 하나의 인공 엑손을 포함하며, 여기에서 상기 공유된 서열은 단일 인공 엑손 서열 중에 포함되고,
상기 NA 분자의 참조 세트는
- 25% 내지 55%의 평균 GC 함량을 가지며,
- 하기의 그룹 중에서 선택된 적어도 5개의 선택적인 전사 사건들을 모방하고:
선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들,
상기 인공 NA 분자의 참조 세트의 엑손 서열의 모든 5' 개시 디뉴클레오티드의 적어도 75%는 GT이며, 상기 인공 NA 분자의 참조 세트의 엑손 서열의 모든 3' 종료 디뉴클레오티드의 적어도 75%는 AT이며,
상기 참조 세트의 서열은 NCBI GenBank 데이터베이스 수납 번호가 표 3 및 표 4 중 어느 하나에 나열된 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)는 10 미만이고, 여기에서 상기 유사성을 하기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정하고: 낮은 복잡성 영역의 필터링, 1차 갭 비용 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기인 단계;
b) 상기 참조 세트를 외부 대조군으로서 상기 전사물 변이체를 포함하는 하나 이상의 샘플에 가하는 단계;
c) NA 서열분석을 리드 생성 및 할당을 근거로 수행하고, 여기에서 참조 리드 할당은 상기 참조 세트의 리드에 의해 생성되고 상기 참조 리드 할당을 사용하여 상기 하나 이상의 샘플의 전사물 변이체의 리드 할당을 조절, 확인 또는 변형시키는 단계
를 포함하는, 하나 이상의 샘플 중 전사물 변이체의 조절된 식별 및/또는 정량분석 방법이다.
참고문헌:
Figure pct00039
Figure pct00040
Figure pct00041
도면의 간단한 설명
도 1: SIRV 설계 원리의 개략적인 개관.
도 2: DNA 합성 후 선택된 SIRV의 플라스미드 선형화에 대한 예시적인 결과. 상기 SIRV는 정확한 크기를 가지며 T7 폴리머라제에 의한 RNA 전사에 사용될 수 있다.
도 3: 선택된 SIRV 및 조건에 대한, T7 폴리머라제에 의한 전사 수율에 대한 예시적인 결과. 전사는 대부분의 선택된 조건에 대해서 성공적이었다. o/n, 밤새.
도 4: KLK5 및 SIRV1 그룹 정렬. 도해는 SIRV1 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV1-100이 마스터 전사물임에 유의한다. SIRV1-101-105는 규정(canonical) 전사물(KLK5 전사물과 유사하게)이다. 전사물 SIRV1-106-109는 인공 전사물이며 이때 후반 3개는 중복(안티센스) 전사물이다. MT = 마스터 전사물.
도 5: LDHD 및 SIRV2 그룹 정렬. 도해는 SIRV2 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV2-100이 마스터 전사물임에 유의한다. SIRV2-201-204는 규정 전사물(LDHD 전사물과 유사하게)이다. 전사물 SIRV2-205 및 206은 인공 모노엑손 안티센스이다. MT = 마스터 전사물.
도 6: LGALS17A 및 SIRV3 그룹 정렬. 도해는 SIRV3 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV3-100이 마스터 전사물임에 유의한다. SIRV3-301-306은 규정 전사물(LGALS17A 전사물과 유사하게)이다. 전사물 SIRV3-307-311은 인공 전사물이며 이때 후반 하나는 모노엑손 안티센스 전사물이다. 전사물 SIRV3-308-310은 중복 안티센스 전사물이다. MT = 마스터 전사물.
도 7: DAPK3 및 SIRV4 그룹 정렬. 도해는 SIRV4 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV4-100이 마스터 전사물임에 유의한다. SIRV4-401-407은 규정 전사물(DAPK3 전사물과 유사하게)이다. 전사물 SIRV4-408-410은 인공 전사물이며 이때 후반 2개는 중복 안티센스 전사물이다. MT = 마스터 전사물.
도 8: HAUS5 및 SIRV5 그룹 정렬. 도해는 SIRV5 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV5-100이 마스터 전사물임에 유의한다. SIRV5-501-510은 규정 전사물(HAUS5 HAUS 전사물과 유사하게)이다. 전사물 SIRV5-511 및 512는 인공 전사물이며 이때 후반 하나는 모노엑손 안티센스 전사물이다. MT = 마스터 전사물.
도 9: USF2 및 SIRV6 그룹 정렬. 도해는 SIRV6 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV6-100이 마스터 전사물임에 유의한다. SIRV6-601-615는 규정 전사물(USF2 전사물과 유사하게)이다. 전사물 SIRV6-616-618은 인공 전사물이며 이때 후반 2개는 모노엑손 안티센스 전사물이다. MT = 마스터 전사물.
도 10: TESK2 및 SIRV7 그룹 정렬. 도해는 SIRV7 및 상응하는 참조 유전자의 전사물 정렬을 도시한다. SIRV7-100이 마스터 전사물임에 유의한다. SIRV7-701-707은 규정 전사물(TESK2 전사물과 유사하게)이다. 전사물 SIRV7-708은 인공 전사물이다. MT = 마스터 전사물.
도 11: SIRV 레이아웃. 모든 SIRV 카세트는 XhoI 제한 부위로 출발한 다음, T7 프로모터, 구아노신 및 SIRV mRNA 바디가 이어진다. 모든 SIRV는 그의 3' 단부에 30 아데노신의 폴리-A 꼬리뿐만 아니라 NsiI 제한 부위를 유지하여 런-오프 전사가 가능하다.
도 12: FPKM 상관관계 플롯. 샘플 1 및 샘플 2의 FPKM 값들이 서로에 대해 플롯팅되어 있다.
도 13: 인공 유전자 SIRV 1의 커버리지를 나타내는 게놈 브라우저 스크린샷. SIRV 1 표지된 전사물을 갖는 것은 모두 주어진 어노테이션에 상응한다. 커프스링크는 오차를 도입하는 Cuff.8 및 .9라 명명되는 5개의 전사물 변이체를 추가로 유도한다.
도 14: 믹스(Mix) E0, E1 및 E2를 수득하기 위한 SIRV 혼합 설계. A) 8개의 프리믹스(PreMix)는, SIRV가 바이오분석기 자취에서 분명하게 식별될 수 있도록 길이가 상이한 6 내지 11개의 SIRV를 함유한다. 2개의 프리믹스를 각각 같은 비로 합하여 총 4개의 서브믹스(SubMix)를 제공하였다. 차례로 이들을 한정된 비로 합하여 최종 믹스 E0, E1 및 E2를 수득하였다. 측정된 자취를 적색으로 나타내며, 서브믹스 및 최종 믹스를 확인하기 위해 상기 프리믹스 자취로부터 계산된 자취를 청색으로 나타낸다.
도 15: 대조군을 갖는 RNA. SIRV 믹스를 또한 시험 준비가 된 참조 RNA 샘플 RC-0, RC-1 및 RC-2로서 입수할 수 있다. 첫 번째 샘플, 보편적인 인간 참조 RNA(UHRR, 10개의 혼주(pooled) 암 세포주로부터, 에이질런트 테크놀로지스 인코포레이티드(Agilent Technologies, Inc.))를 ERCC 엑스폴드(ExFold) 믹스 1로 스파이크하고; 두 번째 샘플, 인간 뇌 참조 RNA(HBRR, 23 공여자의 다수의 뇌 영역으로부터, 라이프 테크놀로지스 인코포레이티드)를 ERCC 엑스폴드 믹스 2로 스파이크하고; 세 번째 샘플의 경우 상기 둘을 2:1 비로 합하였다. 이어서 상기 3개 샘플을 SIRV 믹스 E0, E1 및 E2로 스파이크하여 전체 RNA 중의 2% mRNA 함량과 비교된 상대적인 크기로서 평가된 도면에 나타낸 바와 같은 질량비를 획득하였다.
도 16: SIRV NGS 리드를 정확한 어노테이션 SIRV_C로 할당한 결과로서 SIRV의 투입-산출 상관성, A) E1을 함유하는 샘플 RC-1 및 E2를 함유하는 RC-2에서, B) E2와 E1간의 차별적인 발현 비. 개별적인 데이터 점을 작은 회색 기호로 나타내고 평균값을 큰 흑색 기호로 강조한다. 각각의 라인은 표준 편차를 표시한다. 회색 직선은 대각선을 강조한다.
실시예
실시예 1: SIRV 특징
SIVR(전사물 변이체를 모방하는, 본 발명의 인공 NA 분자)의 특징. 서열번호: 75-148은 각각 서열번호 1-74에 일치하나, 30 아데노신의 폴리-A 꼬리는 없다. "주형 없음"은 상기 SIRV가 직접적인 인간 전사물 모델 주형을 갖지 않고 대신에 단계 E) 내지 G)를 갖는 본 발명의 생성물 방법에 의해 수득될 수 있음을 의미한다. SIRV 그룹은 동일한 인공 유전자의 전사물 변이체를 제공하고 인간 모델 유전자의 조건을 모방한다.
명칭 인간 전사물
주형 		배향 엑손 길이 GC 함량 서열
번호
SIRV1 그룹
SIRV101 KLK5-001 센스 6 1591 46% 1
SIRV102 KLK5-002 센스 4 1330 45% 2
SIRV103 KLK5-004 센스 6 1393 45% 3
SIRV104 KLK5-005 센스 7 1429 45% 4
SIRV105 KLK5-006 센스 5 700 44% 5
SIRV106 주형 없음 센스 3 1003 45% 6
SIRV107 주형 없음 센스, 중복 3 774 45% 7
SIRV108 주형 없음 안티센스, 중복 3 732 46% 8
SIRV109 주형 없음 안티센스, 중복 3 494 45% 9
SIRV2 그룹
SIRV201 LDHD-001 센스 11 2081 42% 10
SIRV202 LDHD-002 센스 11 2001 42% 11
SIRV203 LDHD-003 센스 5 716 41% 12
SIRV204 LDHD-004 센스 3 770 42% 13
SIRV205 주형 없음 안티센스 1 553 42% 14
SIRV206 주형 없음 안티센스 1 454 40% 15
SIRV3 그룹
SIRV301 LGALS17A-001 센스 5 2497 35% 16
SIRV302 LGALS17A-002 센스 2 1837 35% 17
SIRV303 LGALS17A-004 센스 3 2048 35% 18
SIRV304 LGALS17A-005 센스 8 1113 34% 19
SIRV305 LGALS17A-006 센스 3 466 32% 20
SIRV306 LGALS17A-201 센스 3 2403 36% 21
SIRV307 주형 없음 센스 5 809 34% 22
SIRV308 주형 없음 안티센스, 중복 3 509 41% 23
SIRV309 주형 없음 안티센스, 중복 3 826 43% 24
SIRV310 주형 없음 안티센스, 중복 3 619 39% 25
SIRV311 주형 없음 안티센스 1 191 30% 26
SIRV4 그룹
SIRV401 DAPK3-001 센스 9 2283 39% 27
SIRV402 DAPK3-004 센스 3 2089 37% 28
SIRV403 DAPK3-005 센스 4 700 38% 29
SIRV404 DAPK3-006 센스 4 622 38% 30
SIRV405 DAPK3-007 센스 2 656 40% 31
SIRV406 DAPK3-008 센스 2 647 42% 32
SIRV407 DAPK3-201 센스 8 2135 39% 33
SIRV408 주형 없음 센스 5 600 36% 34
SIRV409 주형 없음 안티센스, 중복 3 1597 44% 35
SIRV410 주형 없음 안티센스, 중복 2 980 44% 36
SIRV5 그룹
SIRV501 HAUS5-002 센스 17 1920 45% 37
SIRV502 HAUS5-003 센스 18 2014 46% 38
SIRV503 HAUS5-004 센스 3 556 43% 39
SIRV504 HAUS5-005 센스 1 2503 50% 40
SIRV505 HAUS5-006 센스 16 2059 47% 41
SIRV506 HAUS5-007 센스 2 582 51% 42
SIRV507 HAUS5-008 센스 6 563 50% 43
SIRV508 HAUS5-009 센스 17 2115 46% 44
SIRV509 HAUS5-010 센스 4 915 47% 45
SIRV510 HAUS5-201 센스 18 2504 48% 46
SIRV511 주형 없음 센스 2 576 51% 47
SIRV512 주형 없음 안티센스 1 259 47% 48
SIRV6 그룹
SIRV601 USF2-001 센스 9 1465 42% 49
SIRV602 USF2-002 센스 8 604 41% 50
SIRV603 USF2-003 센스 1 1999 35% 51
SIRV604 USF2-004 센스 10 1567 43% 52
SIRV605 USF2-005 센스 9 1118 43% 53
SIRV606 USF2-006 센스 4 575 45% 54
SIRV607 USF2-007 센스 4 604 47% 55
SIRV608 USF2-008 센스 4 407 35% 56
SIRV609 USF2-009 센스 4 515 48% 57
SIRV610 USF2-010 센스 5 1193 39% 58
SIRV611 USF2-012 센스 3 484 46% 59
SIRV612 USF2-013 센스 10 1558 43% 60
SIRV613 USF2-014 센스 6 1341 38% 61
SIRV614 USF2-015 센스 5 489 40% 62
SIRV615 USF2-016 센스 3 813 34% 63
SIRV616 주형 없음 센스 4 561 45% 64
SIRV617 주형 없음 안티센스 1 306 43% 65
SIRV618 주형 없음 안티센스 1 219 41% 66
SIRV7 그룹
SIRV701 TESK2-001 센스 5 2492 36% 67
SIRV702 TESK2-002 센스 6 2277 37% 68
SIRV703 TESK2-003 센스 5 2528 36% 69
SIRV704 TESK2-004 센스 3 458 29% 70
SIRV705 TESK2-201 센스 5 2492 36% 71
SIRV706 TESK2-202 센스 5 979 33% 72
SIRV707 TESK2-203 센스 10 2356 36% 73
SIRV708 주형 없음 센스 5 919 33% 74
Figure pct00042
Figure pct00043
또한 도 1, 및 4 내지 10을 참조하시오.
예시를 목적으로, SIRV 전사물 그룹 1 내지 7을 생성시키는 7개의 인공 SIRV 유전자(SIRV1-SIRV7)를 서열번호 149 내지 156에 나열한다. 상기 SIRV 유전자는 상기 유전자가 생성시키는 전사물에 의해 한정되므로, 오직 그의 엑손 서열(즉 상기 전사물 중 적어도 하나에서 엑손인 서열, 상기는 다른 전사물에 대해서는 인트론일 수 있다, 즉 존재하지 않을 수 있다)에 의해서 한정된다. 본 명세서에 언급된 바와 같이, 상기는 오직 개념으로서 존재하는 것으로 충분하다.
상기 SIRV의 엑손을 서열번호 156 내지 334에 나열한다.
SIRV는 뉴클레오티드 및 단백질 수준에 대한 블라스트(blast) 검색에 의해 밝혀진 바와 같이 NCBI 데이터베이스 중의 기입물들과 일치성이 없다. 상기 인공 SIRV 전사체로부터 50 nt 길이 NGS 리드를 생성시키는 인실리코 실험에서 상기 SIRVome는 또한 모델 유기체 인간, 마우스, 아라비도프시스 탈리아나(Arabidopsis thaliana), 씨 엘레간스(C. elegans), 디 멜라노가스터(D. Melanogaster), 이 콜라이(E. Coli)(CGA1.20), 에스 세레비지아에(S. Cerevisiae) 및 엑스 트로피칼리스(X. tropicalis)로부터의 어노테이션된 전사체에 그다지 정렬되지 않고, 상기 SIRVome에 매우 충분히 지도화되었다. 또한, 임의의 표적-밖(off-target) 정렬을 리드 스파이크로서 쉽게 식별할 수 있다. 따라서, 상기 SIRV 전사체는 시험된 모델 유기체 전사물과 매우 상이할 것이며 상기 게놈에서 스파이크-인 대조군으로서 사용시 전사물 발견 및 정량분석을 방해하지 않는 듯하다는 결론이 내려진다. 외삽에 의해, 다수의 상이한 계통적 부류로부터의 게놈들이 상기 nt 블라스트 외에 시험되었기 때문에, 상기 인공 SIRV 서열이 임의의 공지된 게놈 시스템을 방해하지 않을 것임은 합리적으로 추정될 수 있다.
SIRV를 또한, ERCC 스파이크-인 전사물에 대한 표적-밖 지도화가 거의 존재하지 않기 때문에, ERCC와 함께 사용할 수 있다.
상기 74 SIRV 전사물은
·NGS RNA-Seq 실험 및 다른 NA 분석 방법, 예를 들어 마이크로-어레이 분석 또는 qPCR에 스파이크-인 전사물로서 사용될 수 있고,
·매우 낮은 표적-밖 정렬과 함께 SIRVome에 대한 독특한 지도화를 허용하는 인공 서열이고,
·길이, GC 함량, 인트론 스플라이싱 부위 디뉴클레오티드 및 엑손-인트론 구조에 관하여 천연 mRNA를 모방하고,
·ERCC와 함께 사용될 수 있고,
·T7 RNA 폴리머라제 전사물로서 비용-효과적으로 생성될 수 있다.
상기 SIRV는
·폴리-A 기준 선택 및 증폭
·동형 검출,
·어노테이션-기반 동형 지도화 및 가설 형성,
·동형 풍부성 평가,
·로그-변화 배수 확인(다양한 SIRV 농도를 갖는 2개 믹스를 사용함으로써),
·동형 풍부성 평가 연산의 훈련(training) 및 확인,
·동형 드노보 조립,
·SQUARE 시스템(WO 2011/095501 A1에 기재된 복잡성 감소 방법)에서 동형 분리
를 허용한다.
실시예 2: SIRV 생성
상기 SIRV를 생성시키기 위해서, 시험관내 전사 주형을 외부 DNA 합성 제공기에 의해 합성하였다. 상기 구조물은 5'에서 3'으로 (a) 독특한 제한 부위(XhoI), 바로 상류의 (b) T7 RNA 폴리머라제 프로모터(그의 3' G는 (c) SIRV 서열의 첫 번째 뉴클레오티드이다), 이어서 이음매 없이 (d)(e) 독점적인 NsiI 제한 부위와 융합된 A(30) 꼬리를 포함한다(도 11).
상기 T7 프로모터의 융합뿐만 아니라 상기 A(30) 꼬리내로의 NsiI 부위의 통합은 5' G(SIRV 서열뿐만 아니라 T7 프로모터의 부분)로 시작하여 여분의 3' 뉴클레오티드가 없는 폴리-A 꼬리로 종료하는 서열-참 RNA를 생성시키는 전사를 허용한다.
DNA 합성 제공기는 벡터, 본래의 T7 프로모터 없는 플라스미드 pUC57내로 클로닝된 유전자 카세트를 전달하였다. 2710 bp 길이의 플라스미드 pUC57은 pUC19의 유도체이며 이 콜라이에서 클로닝 벡터로서 통상적으로 사용된다. 상기 벡터는 암피실린 내성에 대한 bla 유전자 및 백색/청색 선택을 위한 lacZ 유전자를 함유한다. GenBank 수납 번호 Y14837.1, 바이오 베이식 인코포레이티드(Bio Basic, Inc.)에 의해 제공된 지도.
제한 및 전사 분석에 충분한 8 내지 10 ㎍의 각각의 벡터를 수용하였다. XhoI 및 NsiI에 의한 이중 절단은 정확한 삽입 크기 및 제한의 완료를 나타낸다.
그러나, 대규모의 예비 전사를 위해서, 상기 SIRV 플라스미드를 50 ㎍ 배치 규모로 생성시켰다.
플라스미드 선형화:
다량의 RNA를 생성시키기 위한 초기 디폴트 방법은 SIRV 발현 카세트를 함유하는 NsiI 제한된 벡터의 런-오프 전사이다. 이를 위해서, 수 ㎍의 상기 플라스미드를 절단하여 정확한 3' 단부를 획득하였다. 완전한 PstI/NsiI 제한은 모든 구조물에 대해서 바이오 베이식에 의해 이미 입증되었지만, 우리는, 전사의 개시가 시험관내 전사 반응의 제한 단계 중 하나이고 심지어 주형 프렙 중 소량의 환상 플라스미드조차 다량의 전사물을 생성시킬 것이기 때문에, NsiI 단독에 의한 효율적인 절단을 검사하였다(도 2 참조).
NsiI 제한은 3'-돌출 단부를 생성시킨다. 이는 두 번째 가닥 전사를 개시시킬 수 있으며, 이 경우에 우리는 상기 접착 단부의 평활화(blunting)에 의지하였다. 이를 위해서, T4 DNA 폴리머라제의 3'-5' 엑소뉴클레아제 활성을 사용하였다.
에피센터 앰플리스크라이브 키츠 하이일드 앤드 플래시(Epicentre AmpliScribe Kits High Yield and Flash)를 사용하는 T7 전사:
상기 선형화된 전사물을 상업적인 T7 전사물 키트, 에피센터의 앰플리스크라이브 T7 하이일드 전사 키트(Art.No 150408) 및 앰플리스크라이브 T7 플래시 전사 키트(Art.No 150405)에서 주형으로서 사용하였다.
T7 전사를 지배하는 주요 인자는 고 dNTP 농도를 허용하는 전사 조건을 갖는 키트의 사용이다. 이는 고수율을 허용한다, 즉 1 ㎍ 플라스미드는 160 내지 180 ㎍ 이하의 RNA를 생성시킬 수 있다(예를 들어 에피센터의 하이일드 키트).
더욱 또한, 절대 한계까지, 보다 많은 주형은 보다 많은 RNA를 생성시킬 것이다. 다양한 길이의 주형을 위해서, 몰농도를 고려해야 하며, 짧은 주형은 전사 개시가 제한 단계이고 T7 폴리머라제 연장의 한 단계가 600 nt까지 포함하므로(에피센터의 웹사이트로부터의 정보) 보다 긴 것과 동일한 질량의 RNA를 생성시키지는 않을 것이다.
보다 긴 배양 시간은 상기 개시 가능성을 증가시키고, 보다 짧은 주형의 수율에 보다 큰 영향을 미친다. 따라서 때때로 표준 2시간이 아니라 4 내지 6시간 또는 심지어 밤새 배양하는 것이 권장된다. 그러나, 보다 긴 배양은 상기 T7 전사 완충제가 Mg2 + 양이온을 함유하기 때문에 RNA 분해를 생성시킬 수 있다.
상기 T7 전사효소 반응 온도를 37 ℃에서 42 ℃로 증가시키면 수율이 강하게 증가할 수 있다. 이는 보다 복잡한(GC-풍부, 구조화된) 주형의 경우 현저할 수 있다(도 3 참조).
미량의 GuSCN, 페놀, SDS, RNA 또는 금속 이온은 T7 전사효소 활성을 억제할 수 있다. 예를 들어 와트만 정제에 의한 상기 선형화된 플라스미드의 엄격한 정제가 권장된다. 한편으로, 상기 반응 부피를 증가시키거나 또는 상기 플라스미드 투입 부피를 낮출 수 있다.
주형 DNA를 DNase에 의해 제거해야 한다. 에피센터(앰플리스크라이브 매뉴얼)에 따라, 1 단위의 상기 포함된 DNase를 37 ℃에서 15분 동안 추가로 배양하면서 상기 전사에 직접 가할 수 있다. 상기 DNase 처리를 RNA 완전성에 대한, 즉 상기가 잔류 RNase로 인해 RNA를 분해시키는지에 대한 영향에 대해 시험할 것이다. 한편으로, DNA를 또한 SPLIT 프로토콜 변이체에서 산성 페놀 추출에 의해 제거할 수 있다. 그러나, GuSCN은 후속의 실리카 컬럼 결합에 필요하지 않을 수도 있다.
남아있는 플라스미드 DNA를 생물분석기 실행(심지어 RNA-특이성 염료와 함께)에서 또는 -정량적으로- qPCR 분석에서 프라이머 GCTAATACGACTCACTATA*G (서열번호 337) 및 TTTTTTTTTTTTTTTTTTTTTTTTT*V (서열번호 338)를 사용하여 검출할 수도 있으며, 이때 (*)는 포스포티오에이트 결합을 갖는 뉴클레오티드이다.
권장된 SIRV 정제 방법을 하기에 기재한다. PAGE: NGS 스파이크-인 전사물에 필요한 높은 품질의 시험관내 전사된 RNA를 정제하기 위한 표준 프로토콜은 PAGE 용출이나, 상기는 성가시며 그다지 정확하지 않고, UV 가교결합을 유도할 수도 있고, >1 kb 전사물에는 적합하지 않다.
실리카-기반 정제:
정제를 처음에는 단지 핵산으로부터 dNTP, 첨가제 및 단백질을 제거하는 것으로 당해 분야의 모든 숙련가에게 공지된 와트만 프로토콜에 의해 수행할 것이다. 그러나, 상기 과정은 손실 경향이 있으며; 시험 마커의 60% 이하가 상기 표준 과정에서 용출되지 않았다. 또한, 상기 DNA 주형이 함께 용출될 것이다. 용출 완충제 EB 또는 저장 완충제 SB가 효율적인 용출에 사용될 수 있는지를 시험해야 한다.
전사물의 자기 올리고(magnetic oligo)(dT) 비드 정제:
전사 반응이 완전길이 RNA(NsiI 제한 부위까지)를 생성시키지 못하는 경우, 상기 RNA는 A(30)-꼬리를 함유하지 않을 것이다. 따라서, 올리고(dT) 비드 정제를 사용하여 완전-길이 전사물을 선택적으로 정제할 수 있다. 그러나, 상기 방법은, 상기 RNA가 또한 상기 A(30)-신장부의 최소 하나의 사본을 함유하기 때문에, 예행 전사(run-through) 또는 두 번째 가닥 전사에 의해 생성된 이상(aberrant) RNA를 구분하지 못할 것이다. 상기 DNA 주형의 하나의 가닥이 또한 상기 폴리-A 신장부를 함유할 것임에 유의한다. DNA가 그의 dsDNA 형태로 존재하는지의 여부(상기 전사물이 그의 주형으로부터 방출되기 때문에) 및 올리고(dT) 하이브리드화에 참여할 수 없는지를 결정할 필요가 있다. 상기 방법의 하나의 변형에서 상기 올리고는 RNA일 것이며, 상기 결합 단계에 이어서 RNase H 절단, 그의 암호화된 A(30) 신장부를 통해 비드에 결합된 임의의 플라스미드 DNA의 제거가 이어질 수 있다. 한편으로, 상기 DNA를 DNase 처리에 의해 제거한다.
피핀 프렙(pippin prep):
세이지 사이언티픽 피핀 프렙(Sage Scientific Pippin prep)은 1.5% 또는 2% 고유 아가로스 카세트로부터 dsDNA(예를 들어 NGS 라이브러리)를 용출시키기 위해 고안된 자동화된 젤 용출 시스템이다. RNA는 상기 피핀 프렙의 외부 또는 내부 DNA 표준에 따라 실행되지 않으므로, 길이 평가가 가능하지 않다. 그럼에도 불구하고, 충분한 순도의 SIRV는 설정 한계 염기쌍 값 뒤의 다음 피크를 자동적으로 수집하는 크기 선택 프로토콜 "피크"에 의해 검출될 수 있는 단일의 주요 피크로 진행한다.
품질 조절 및 정량분석은 SIRV 혼합물의 생성에 중요하다. 나노드롭 정량분석: 광도계 측정은 A260/A230 및 A260/A280 비 형태로 농도(및 따라서 수율) 및 순도를 제공한다. 중요하게, 불충분한 정제는 나노드롭 장비(나노드롭 인스트루먼츠)에서 수행되는 바와 같은 흡광도 측정이 미량의 dNTP를 또한 측정하기 때문에(이는 260 ㎚에서 과도한 흡광도를 갖는다) 흡광도 측정으로서 문제가 있다. 큐빗(Qubit) 측정(라이프테크놀로지스)을 세 번째 참조로서 취할 수 있다.
에이질런트 생물분석기 RNA 나노칩:
상기 SIRV 전사물을 올바른 길이, 양, RNA 완전성(즉 파괴 또는 분해 산물) 및 이상(보다 긴) 생성물에 대해서 에이질런트 생물분석기 RNA 칩상에서 평가할 수 있다.
변성 젤 전기영동:
상기 생물분석기에 상보적으로 RNA를 또한 그의 크기에 따라 변성 PAA 또는 아가로스 젤상에서 분석할 수 있다. 이는 전사물 길이의 보다 정확한 평가를, 정량분석 및 상기 생물분석기에 의해 제공된 범위 없이 가능하게 할 수 있다.
qPCR:
상기 스파이크-인 전사물의 완전성을 평가하고 상보적인 정량분석을 유도하기 위해서, 완전-길이 cDNA 합성에 이어서 상기 전사물의 5', 중간 및 3' 영역에 위치한 다중 앰플리콘의 qPCR을 수행할 수 있다. 외부 표준으로서, 상기 PCR 전사 주형을 동일한 셋업에서 증폭시킬 수 있다. 이러한 셋업을 또한 SIRV 믹스 중의 상대적인 농도의 측정에 적용할 수 있다.
이들 SIRV-특이성 프라이머를 오직 하나의 특이적인 SIRV 각각을 표적화하고 예를 들어 주어진 유전자의 모든 SIRV에 공통적인 엑손은 표적화하지 않도록 조심스럽게 설계할 필요가 있다.
실시예 3: RNA - seq에서 외부 대조군으로서 SIRV의 용도
하기의 단계 i) 샘플 수집, ii) RNA 정제, iii) NGS 라이브러리 생성, iv) NGS 서열분석, v) 참조 어노테이션에 대한 리드 정렬 및 vi) 후속의 생물정보학 처리로 이루어지는 실험 과정이 상대적인 전사물 풍부성을 계산하는 것으로 널리 추정된다. 그러나, 하기의 실시예에 나타내는 바와 동일한 실험 데이터 세트의 상이한 방법들, 예를 들어 상이한 샘플 제조 및 또한 생물정보 처리 루틴이 또한 가능하다.
부분적으로 확인된 전사물 풍부성을 함유하는 단지 매우 작은 데이터 세트를 입수할 수 있다. 상기 중 하나는 마이크로-어레이 품질 조절(MAQC) 샘플(MAQC Consortium, 2006)로부터의 것이고 보편적인 인간 참조 RNA(UHRR) 및 인간 뇌 참조 RNA(HBRR)를 함유한다. 상기 두 RNA 샘플 모두에 대해서 qPCR 측정이 1044 Taqman 탐침으로 유도되었다. 상기 측정치를 수납 번호 GSE5350하에서 유전자 발현 옴니버스로부터 입수할 수 있다. 또한, 상기 UHR 및 뇌 RNA 샘플을 일루미나 게놈애널라이저(Illumina GenomeAnalyzer)의 7개 레인상에서 서열분석하여, 35 bp 단일-단부 리드를 생성시켰다(James et al., 2010). 상기 리드(NCBI 리드 기록 보관소로부터 수납번호 SRA010153으로 입수할 수 있다)를 Ensembl 어노테이션 GRCh37 버전 75에 대해 톱햇2로 지도화하였다. 상기 1044개 Taqman 탐침으로부터 오직 906개 탐침만이 유지되었으며, 이를 GSE5350에 따라 단일 Refseq 어노테이션에 대해 지도화한다. 상기 Ensembl 어노테이션이 상기 실험에 사용되었기 때문에 상기 Taqman 탐침 세트는 Ensembl 중에 특유의 당량을 갖도록 Taqman 탐침의 Refseq 어노테이션을 요청함으로써 추가로 감소되었다. 최종적으로, 상기 894개 Taqman 탐침으로부터, 오직 Ensembl 전사물 어노테이션이 다수의 전사물을 갖는 유전자내에 함유된 것들만이 사용되었다. 그 결과 최종적으로 798개 Taqman 탐침 세트가 생성되었다. Pennseq(Hu et al., 2014), 방법 1, 및 편향 보정되거나 보정되지 않은 커프스링크, 방법 2 및 3(Roberts et al., 2011; Trapnell et al., 2010)을 사용하여 상기 798개 전사물상에서 FPKM 값 형태로 농도 평가를 유도하였다.
상기 상이한 방법들에 의해 획득된 FPKM 값과 qPCR 값 사이의 상관성을 표 7에 나타낸다. 상기 상관성을 로그 공간에서 R2 값 및 스피어맨 상관성 ρ로 측정한다. 0에 가까운 값은 로그 공간에서 통계학을 현저하게 왜곡시킬 수 있기 때문에, 1e-3 이하의 FPKM 값을 상기 모든 방법들에 대해서 1e-3으로 설정한다. 한편으로, 1e-3 이하의 FPKM을 갖는 전사물은 검출되지 않은 것으로 간주할 수 있다.
UHR RNA 레인 SRR037445상의, FPKM과 qPCR간의 상관성 및 검출되지 않는(ND) 전사물, 즉 FPKM < 1e-3을 갖는 전사물의 성질
R2 ρ ND % ND avg log10 (qPCR)
Pennseq 0.418 0.7129 2.79 -1.6506
커프스링크 0.3317 0.6541 15.48 -1.6801
편향 보정된
커프스링크		 0.3943 0.7312 14.61 -1.7606
표 7에 나타낸 바와 같이, 상기 R2 값은 한편으로 Pennseq의 경우 0.418이고, 편향 보정되지 않은 커프스링크의 경우 0.3317이며 편향 보정된 커프스링크의 경우 0.3943이다. 다른 한편으로, 상기 스피어맨 상관성은 Pennseq의 경우 0.7129이고, 편향 보정되지 않은 커프스링크의 경우 0.6541이며 편향 보정된 커프스링크의 경우 0.7312이다. 현저하게, 편향 보정된 및 보정되지 않은 커프스링크는 qPCR에 의해 존재하는 것으로 입증된 전사물의 14.61% 및 15.48%를 검출하지 않는 반면, Pennseq는 각각 2.79%를 검출하지 않는다. 중요하게, 상기 3개의 계산 방법에 의해 측정되지 않은 전사물은 qPCR 확인 실험에서 -1.65 내지 -1.76의 높은 평균 log10 풍부성을 가졌다.
본 실시예는 2개의 상이한 생물정보학적 연산, 및 2개의 상이한 편향 보정을 갖는 것(커프스링크)이 3개의 현저하게 상이한 결과를 생성시킨다는 하나 초과의 Ensembl 전사물 어노테이션을 함유하는 798개 Taqman qPCR 확인된 유전자좌의 선택을 통해 설명한다. 상기 정렬은 다수의 유전자내의 리드를 잘못된 전사물로 분배한다. 절대 상관성은 지면 저점(ground trough)을 우리가 알지 못하기 때문에 불가능하다. 오직 천연 유전자 중의 전사물과 유사한 복잡한 환경에 존재하는 공지된 풍부성을 갖는 인공 전사물 변이체만이, 상기가 개별적인 단계든 전체 작업공정이든, 측정 방법 정확성의 정량적인 평가를 가능하게 한다.
실시예 4: 랜덤 분포를 시험하기 위한 카이 -제곱 검정
예로서, "GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖는 인공 전사물 서열의 세트"에 대한 카이-제곱 검정을 어떻게 적용하는지에 대해 설명할 것이다.
별개의 경우, 또는 세포의 수(n): 16 (GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT)
인공 전사물 서열의 수(N): 74
5' 개시 뉴클레오티드(O1, O2, O3, ..., On)의 존재(수):
GAA 5 GAC 5 GAG 4 GAG 6 GAT 3 GCA 2
GCC 4 GCG 5 GCT 6 GGA 7 GGC 4 GGG 3
GTA 4 GTC 5 GTG 6 GTT 5
자유도(df): n - p = 15(별개의 균일한 분포의 경우 p = 1)
임의의 세포에 대한 예상 존재는 (별개의 균일한 분포의 귀무가설하에서) Ei = N/n = 4.625이다. 이는 트리뉴클레오티드의 (완벽한) 균일한 분포를 갖는 세트가 허위로 5' 개시 트리뉴클레오티드로서 4.625의 각각의 상기 언급된 트리뉴클레오티드를 가짐을 의미한다.
카이-제곱(피어슨의 누적 검정 통계학)을 하기와 같이 정의한다:
Figure pct00044
상기에서 상기 식에 직접 적용된 Oi, Ei 및 n에 대한 값은 카이-제곱 = 5.57을 생성시킨다
일정한 카이-제곱 값(본 실시예에서 5.57) 및 일정한 자유도(본 실시예에서 15)에 대한 확률값("p 값")을 주지된 표(소위 카이-제곱 표)에 표로 나타낸다. 상기 p 값을 또한 널리 사용되는 사무용 소프트웨어, 예를 들어 마이크로소프트 엑셀, 리브레오피스 또는 오픈오피스(이들 중 후반 2개는 무료로 입수할 수 있다)에 의해, 또는 무료로 입수할 수 있는 R 소프트웨어 패키지로 계산할 수 있다. 마이크로소프트 엑셀 2003의 영문 버전에서, 상기 기능을 CHIDIST라 칭한다.
카이-제곱 = 5.57 및 df = 15와 관련된 p 값은 0.9861이다. 따라서, 본 실시예에서 개시 뉴클레오티드의 존재는 본 명세서에 정의된 바와 같은 "필수적으로 랜덤하게 분포된다"는 조건을 충족시킨다.
실시예 5: SIRV 평가
서열번호 1 내지 74에 의해 제공된 상술한 세트로부터의 74개 SIRV 중 60개를 합성하고, 클로닝하고, 발현시키고, 정제하고, 품질 조절하고 전기영동 측정(RNA 나노 및 피코 칩 및 생물분석기(에이질런트)에서의 분석)을 통해 농도를 측정한 후에 2개의 마스터 믹스로 합하고 추가의 샘플 제조를 위해 10 ng/㎕ 이상의 농도로 농축시켰다. SIRV 믹스 1은 동일한 질량으로 모두 60개의 SIRV를 함유하였다. SIRV 믹스 2를 1:10:100의 랜덤화된 비로 102 배 이하까지 SIRV 유전자내 개별적인 SIRV의 양을 변화시킨 혼합 설계에 따라 제조하였다. 상기 SIRV 믹스 2에서 각각의 SIRV 유전자는 모든 부차적인 SIRV들의 합으로서 동등한 질량으로 제공되었다.
3개의 RNA 샘플을 제조하였다. 샘플 1은 단지 일반적인 SIRV 전사물 혼합물 SIRV 믹스 1(100 ng)만을 함유하였다. 샘플 2는 500 ng의 보편적인 인간 참조 RNA(에이질런트)를 0.3 ng ERCC(앰비온) 및 3 ng SIRV 믹스 1과 합하였다. 샘플 3은 500 ng 의 보편적인 인간 참조 RNA(에이질런트)와 0.3 ng ERCC(앰비온) 및 3 ng SIRV 믹스 2로 이루어졌다.
상기 3개의 mRNA 샘플을, 샘플 제조를 수행하고 서열분석을 수행하는 서비스 제공자(파스테리스(Fasteris), 스위스 소재)에게 선적하였다. NGS 라이브러리를 폴리A 선택 없이 맞춤 라이브러리 제조에 의해 샘플 1로부터 제조한 반면, 샘플 2 및 3은 폴리A 선택과 함께 일루미나 가닥 mRNA 라이브러리 제조를 겪었다. 3개의 라이브러리 모두 바코드를 붙였고, 의도된 균등한 비로 혼합하였다. 서열분석을 v3 화학물질을 갖는 일루미나 MiSeq상에서 수행하였으며 150 bp의 색인이 달린 리드가 생성되었다.
종합해서, 26.7 Mio 리드가 생성되었고 주어진 바코드로 할당될 수 있었다. 상기 리드의 품질을 FastQC(v0.11.1)로 평가하였다. 일부 어댑터 오염이 검출되었으며 이를 하기의 매개변수: ./bbduk.sh ... ktrim = r k=28 mink=12 hdist=1 minlength=20을 갖는 bbmap 스위트(버전 32.32)로부터 bbduk를 사용하여 손질할 수 있었다. 상기 생성 리드를 Ensembl의 GRCh 37.75, 앰비온의 ERCC92 및 SIRVome의 결합된 전사체 및 게놈 참조 어노테이션에 대해 톱햇(v.2.0.8)으로 지도화하였다. 상기 지도화 통계학을 표 8에 나타낸다.
지도화 통계학
　 전체 리드 지도화 리드 [%] 특유의 지도화 리드 [%]
샘플 1 10,246,442 8,585,641 83.79 8,505,344 83.01
샘플 2 10,119,416 8,642,852 85.41 8,399,336 83.00
샘플 3 6,308,855 5,404,486 85.67 5,268,757 83.51
상이한 어노테이션에 걸친 특유의 지도화 리드의 분포를 표 9에 제공한다. 샘플 2 및 샘플 3에서 하기의 리드 비 70.3:2.7:27의 UHRR:ERCC:SIRV가 중량 투입에 따라 예상되었으며 전체 RNA 중 2% mRNA 함량이 추정되었다.
특유의 지도화 리드의 분포
　 GRCh37.75 ERCC92 SIRV
샘플 1 4,330 0.05% 11 0.00% 8,505,555 99.949%
샘플 2 7,521,308 89.55% 38,031 0.45% 839,997 10.00%
샘플 3 4,156,399 78.89% 22,207 0.42% 1,090,151 20.69%
샘플 1에서, 전체 리드의 99.94%의 매우 높은 수가 SIRVome로 지도화된 반면 단지 0.06%만이 인간 게놈 전체 및 ERCC로 지도화되었다. 상기 결과는 상기 SIRVome와 다른 공지된 서열과의 높은 부적합성 및 SIRV 서열의 독특함을 입증한다.
샘플 2 및 3에서, 상기 92 ERCC 중 58 및 52가 모든 리드의 0.45 및 0.42%에 상응하게 검출되었다. 중량 기준으로 상기 가중된 3% 이하의 ERCC 리드의 반복되는 과소-표시는 단지 24 아데노신의 비교적 짧은 폴리-A 꼬리 및 잠재적으로 가수분해되거나 또는 달리 단편화되고 폴리-A 선택되고 고갈된 ERCC에 기인한다. 상기 SIRV를 상기 ERCC에 비해 10배 접근으로 상기 샘플에 혼합하였으며, 상기는 30개 아데노신의 보다 긴 폴리-A 꼬리 및 상기 SRIV의 잠재적으로 보다 높은 완전성에 의해 야기된 10 및 20.7% 및 따라서 20- 내지 40-배 접근을 따라잡았다.
상기 지도화된 리드를 IGV 게놈 브라우저를 사용하여 시각적으로 검사하였다. 편향 보정된 커프스링크(v.1.3.0)를 사용하여 상기 전사물 풍부성을 평가하였다. 모든 SIRV 전사물을 FPKM 값>0으로 검출하였다. 0.8 이하의 R2 값을 갖는 투입-산출 상관성은, 광범위한 품질 측정이 삽입성 형광 염료를 사용하는 예비 모액 농도 측정 외에 다수의 독립적인 수단에 의해 지면 저점 투입 농도를 확인하기 위해 필요함을 입증한다. qPCR 및 Taqman 분석은 상기 농도의 각각의 확인을 위해 준비 중이다.
도 12는 샘플 2 대 샘플 1의 커프스링크 계산된 상대적인 농도 값의 상관성을 도시한다. 샘플 2의 SIRV 농도는 물론 UHRR 및 ERCC 배경으로 인해 대략 10배 더 낮다. 그럼에도 불구하고, 0.95 이상의 높은 R2 값이 예상될 수 있었는데, 그 이유는 일치하는 SIRV 믹스 1이 상기 두 샘플 모두에서 측정되었기 때문이다. 부분적으로 거짓인 리드 할당은 도 13에 도시된 바와 같이 생물정보학적 처리에 의해 야기된다.
SIRV 유전자 1에 대한 전체적인 커버리지를 도 13의 상부 열에, 하기의 식별된 어노테이션된 전사물 SIRV 101 내지 109(모두 SIRV1으로 암호화됨)와 함께 나타내나, 단 SIRV 믹스 1에서 74개 SIRV 중 60개의 부분이 아니고 따라서 상기 어노테이션에 포함되지 않는 105는 제외한다. 커프스링크는 추가적인 전사물 가설을 더하고 리드를 내부적으로 한정된 길이 의존성 확률 분포 및 다른 다수의 할당 규칙에 따라 전사물 변이체의 세트로 할당하였기 때문에, 제공된 값들은 0.83의 R2 값을 갖는 샘플 1 및 2 사이의 SIRV 상관성이 일치하는 샘플들에 대해 낮을 때 단순히 정확하지 않다.
상기 수행된 할당 오차의 평가를 위해서 오직 상기 제공된 SIRV 참조 세트에 의해서만 가능한 투입 농도의 지면 저점을 아는 것이 필수적이다. 상기 주어진 모델 복잡성에서 오직 상기 투입-산출 상관성의 분석만이 상기 측정의 정확성에 관한 가정을 미지의 전사물 변이체의 전체 세트에 대해 외삽할 수 있게 하며, 이는 본 발명에 의해서 처음으로 가능하다.
실시예 6: 한정된 농도 및 농도비를 갖는 SIRV 믹스 E0, E1, 및 E2의 제조, 및 RNA 샘플 RC -0, RC -1 및 RC -2를 스파이크하기 위한 SIRV 믹스의 용도
여기에서, 상기 74개 SIRV 중 69개 SIRV를, 모세관 전기영동 생물분석기 자취에서 정확하게 계산된 크기의 주 피크 중 ≥85 w/w%를 나타냄으로써 한정된 바와 같은 순도로 획득된 것으로서 선택하였다.
상기 SIRV 용액을 흡광도 분광학(나노드롭, 써모 사이언티픽)에 의해 측정하고 모액 농도를 ≥50 ng/㎕로 조절하였다. 260 ㎚ 대 280 ㎚ 및 260 ㎚ 대 230 ㎚에서의 흡광도 비는 상기 RNA의 최고 순도를 가리키며 하기와 같이 기록되었다:
A260 nm / 280 nm 2.14 ± 0.12,
A260 nm / 280 nm 2.17 ± 0.20
상기 나노드롭은 정확한 RNA 정량분석을 허용하며, 제조사의 명세서에 따른 오차는 ≤100 ng/㎕ 핵산 샘플의 경우 ±2 ng/㎕이다. 50 ng/㎕ 부근의 최종 SIRV 모액 농도 측정의 정량분석에 대한 상대적인 오차는 ±4%이다.
각 용액의 몰농도를 하기에 따른 SIRV 서열의 기본 분포를 근거로 계산하였다:
MW [g/mol] = A*329.2+U*306.2+C*305.2+G*345.2+159
8개의 프리믹스를 동몰비로 6 내지 11개의 SIRV 전사물을 함유하도록 설계하였다. 이들의 길이 분포는 도 14A에 나타낸 바와 같이 생물분석기 자취에서의 특유의 식별을 허용하여 상기 프리믹스 및 후속의 믹스 중 SIRV의 존재 및 완전성을 모니터할 수 있게 한다(도 14B 및 C). 상기 생물분석기 자취는 절대적인 정량분석을 허용하지 않지만, 상기를 사용하여 상대적인 화합물 분포 및 상기 혼합 과정의 일관성을 추적하였다.
상기 8개 프리믹스의 정확한 부피측정 제조를 계산된 표적 농도로부터 0.002%±3.4%(최대 7.6%)의 편차를 갖는 나노드롭 농도 측정에 의해 조절하였다. 상기 부피의 혼합을 분석 저울상에서의 칭량에 의해 추가로 모니터하였으며, 이는 1.8%±0.65%(최대 2.5%)의 편차를 보였다.
상기 8개의 프리믹스를 짝을 이루어 합하여 4개의 서브믹스를 제공하였다. 상기 혼합 과정을 도 14B에 나타낸 바와 같이 전기영동에 의해 품질 모니터하였다. 상기 4개의 서브믹스의 부피측정 제조를 나노드롭 농도 측정(0.8%±2.5%, 최대 4.5%의 편차)에 의해 조절하였다.
상기 4개의 서브믹스를 한정된 부피측정비로 최종 믹스에 합하였으며, 전기영동에 의한 상기 혼합 과정의 모니터링을 도 14C에 나타낸다. 상기 4개의 서브믹스를 최종 믹스 E0에 합하는 비는 1:1:1:1이었으며, 최종 믹스 E1의 경우 1/4:1/2:2:1이고, 최종 믹스 E2의 경우는 4:1/4:1/32:1이었다. 나노드롭 농도 측정은 상기 계산된 표적 농도로부터 5.1%±3.3%(최대 8.6%)의 편차를 나타내었다.
매우 좁은 여유분 내에서, 믹스의 모든 생물분석기 자취는 그들 각각의 프리믹스 및 서브믹스 구성성분들의 합을 닮는다(도 14). 상대적인 피크 모양 및 위치는 SIRV 믹스에 대한 신뢰성 있는 정량적인 모니터링 도구이다.
이러한 수단에 의해서 신뢰성 있는 SIRV 농도 및 농도비가 상이한 혼합물들에서 보장될 수 있다.
SIRV 믹스 E0, E1 및 E2를 사용하여 보편적인 인간 참조 RNA(UHRR) 및 인간 뇌 참조 RNA(HBRR)(또한 ERCC 대조용 믹스 1 및 2를 함유하여 대조용 RC-0, RC-1 및 RC-2를 갖는 참조 RNA를 생성시켰다)를 스파이크하였다. 상기 각 RNA 분획의 상대적인 양을 도 15에 나타내며 이는 상기 UHRR 및 HBRR 중의 전체 RNA의 2%의 일정한 mRNA 함량을 근거로 계산되었다. 상기 스파이크-인, SIRV 및 ERCC 믹스의 최종의 상대적인 농도는 상기 참조 RNA의 진정한 mRNA 함량뿐만 아니라 리보솜 및 다른 매우 풍부한 RNA의 양을 감소시키면서 고갈 및/또는 농축시키는 방법에 따라 변한다. 이러한 샘플은 상이한 RNA-Seq 워크 플로우(work flow)를 시험하기 위해 고안되었다.
실시예 7: NGS 서열분석, SIRV 믹스를 갖는 RNA 샘플 RC -1 및 RC -2의 데이터 평가 및 상이한 어노테이션을 사용함에 의한 상기 RNA 서열분석 파이프라인의 정확성의 측정
폴리-A-꼬리가 없는 SIRV 분자의 서열번호 1 내지 74 및 모든 엑손의 서열번호 156 내지 334의 서열들은 임의의 공통의 어노테이션 파일 포맷으로 전치될 수 있는 순수한 SIRV 서열들이다. 하나의 상기와 같은 예는 모든 엑손, 인트론 및 상기 첫 번째 및 마지막 엑손에 인접하고 번역되지 않은 영역들라 지칭되는 서열의 순수한 뉴클레오티드 서열을 나열하는 FAS-TA-파일, 및 각 엑손의 개시 및 종료 좌표에 관한 정보를 보유하는 상응하는 GTF-파일의 조합이다. 서열번호 156 내지 334 서열을 인간 모델 유전자의 배향에 상응하는 가닥 배향으로 전치하였으며, 모든 인트론 서열을 각각의 길이의 GC-가중된 랜덤 서열로 충전하였으며 이때 모든 인트론 공여체-수용체 부위는 표 2에 나타낸 바와 같은 규정 및 비-규정 공여체 수용체 쌍에 대한 그들의 상대적인 존재에 상응한다. 서열번호 339 내지 345(7개 서열을 갖는 FASTA 파일을 나타낸다)는 상기 완전한 엑손 및 인트론 서열을 1 kb 길이 상류 및 1 kb 길이 하류 서열과 함께 함유한다. 상기 GTF 파일은 상기 변이체 구조에 관한 정보를 함유하며 하기의 변화를 예로서 제공한다, GTF 파일 "SIRV C"(부록 B에 나열됨)는 믹스 E1 및 E2 중에 있는 모든 SIRV의 정확한 어노테이션을 함유한다. GTF 파일 "SIRV I"(부록 A에 나열됨)는 불충분한 어노테이션의 다수의 가능성들 중 하나이다. 여기에서, 믹스들 중에 실제로 존재하는 일부 SIRV는 어노테이션되지 않는다. GTF 파일 "SIRV O"(부록 C에 나열됨)는 무한한 수의 가능한 과-어노테이션 중 하나이다. 추가적인 SIRV가 어노테이션되며, 이들은 믹스 중에 존재하지 않는다. 본문에서 상기 어노테이션의 변화를 SIRV_C, SIRV_I 및 SIRV_O라 칭한다.
상기 SIRV를 사용하는 데이터 평가의 가능성은 다양하다. 하기의 제안은 RNA-Seq 파이프라인의 수행성능을 평가하기 위해 수행되어야 하는 기본적인 과정을 개략한다. 역다중화, 바코드 및 품질 손질 후에, 리드를 각각의 게놈, SIRVome(모든 SIRV 서열 전체), 및 적용 가능한 경우 ERCC 서열에 지도화해야 한다. 상기 SIRVome에 지도화되는 모든 리드를 여과하여 별도로 처리할 수 있다.
리드의 유전자 부류에 대한 할당은 상기 스파이크-인 과정의 변화성에 관한 첫 번째 개관을 제공한다. 상기 SIRV 함량은 그의 예상되는 질량 또는 몰비율과 관련이 있어야 한다. RNA 분자의 길이를 포함하고 FPKM과 같은 척도에 이르기 위한 라이브러리 제조의 경우 SIRV 리드의 비율은 질량비에 따라야 하는 반면, 어느 한 태그가 독립적으로 RNA 분자를 카운트하는 라이브러리 제조의 경우 상기 SIRV 리드는 몰비에 따라야 한다. 샘플-특이적인 편향의 보정은 차별적인 발현(DE) 분석에 중요하다. RNA 샘플 배경, mRNA 농도 및 완전성의 변화, 및 고갈 및/또는 mRNA 농축 과정의 변화는 서열분석된 라이브러리 중에 상이한 SIRV 믹스 함량을 유도한다. 전체 RNA 샘플의 mRNA 함량은 2.5 이하 또는 그 이상의 인자만큼 변할 수 있다. 상기와 같은 편향의 보정은 차별적인 발현의 정확한 시험, 및 후속적으로 RNA 샘플 자체 중의 상기 DE 측정의 상대화 및 보정에 중요하다. 상쇄 인자(offset factor)는 RNA 부류 분포의 척도이며 SIRV 대조군-기반 표준화에 사용될 수 있다. 상기 SIRV 믹스의 조심스러운 정량적인 스파이크-인 과정은 필수적인 선결요건이며 샘플 정량분석에 하류인 정확한 부피측정 샘플 처리를 요한다. 모든 측정 및 후속적인 정규화는 작은 부피 규모로 실행시 성취가능한 피펫팅 정확성과 같은 명백한 실험 변수와 관련하여 설정될 필요가 있다.
일례로 NGS 라이브러리의 3중 복제물을 TruSeq 스트랜디드 mRNA 라이브러리 프렙 키트(일루미나, 인코포레이티드)를 사용하여 RC-1 및 RC-2의 500 ng 투입 RNA로 생성시킨 후에 6개의 바코딩된 라이브러리를 HiSeq 2500상에서 공칭 125 bp 길이의 짝진 단부 서열분석 실행으로 서열분석하여, 각각 RC-1 3중 복제물의 경우 16.27±0.16 Mio 손질된 유지된 짝진 단부 리드, 및 RC-2 3중 복제물의 경우 16.97±1.45 Mio를 획득하였다. 상기 리드를 톱햇2로 인간 참조 게놈, ERCC 서열 및 SIRV 서열에 대해 지도화하였다. 상기 SIRV에 속하는 리드의 상대량은 샘플 RC-1에서 2.32±0.05%, 및 샘플 RC-2에서 1.87±0.12%인 것으로 측정되었다.
도 15에서, 스파이크-인 SIRV의 비는 전체 RNA 중 추정된 2% 평균 mRNA 함량에 대해 보다 양호한 비교를 위해 제공된다. 그러나, 진정한 mRNA 함량은 가변적인 것으로 공지되어 있다. 상기는 UHRR에서 3% 및 HBRR에서 2%에 가까운 것으로 이전에 측정되었다(Shippy et al., 2006). mRNA 비 HURR/HBRR은 1.5인 것으로 예상된다.
샘플 RC-2의 참조 RNA 배경은 RC-0 참조 RNA 배경의 2/3 및 RC-1 참조 RNA 배경의 1/3을 함유하기 때문에 상기 RC 샘플 RC-1 및 RC-2 중 2개 SIRV 측정은 상기 UHRR 참조 RNA(샘플 RC-0 중; 상기 참조) 중의 mRNA 함량을 계산할 수 있게 한다. 상기 SIRV는 샘플 RC-1에 2% mRNA에 비해 2.53%로 스파이크되었고, 2.32%로 측정되었으며 이는 HBRR mRNA 함량이 2.18%인 값을 생성시키며, 샘플 RC-2 중의 mRNA 함량은 2.89%이고, 이는 3.44%로 상기 UHRR mRNA에 대해 계산된 값을 유도한다. 상기 mRNA 비 UHRR/HBRR은 1.58인 것으로 측정되며, 이는 1.5의 앞서 공개된 비를 증명한다. 상기 SIRV는 폴리(A30)-꼬리가 상기 사용된 mRNA NGS 라이브러리 제조의 일부인 폴리-A-농축 방법에서 정량적인 표현에 충분함을 입증하는 스파이크된 비를 근거로 100%에 가깝게 나타난다.
커프스링크2 연산에 의한 SIRV 리드의 할당을 SIRV_C 어노테이션을 사용하여 수행하였다. 상기 풍부성은 리드 할당을 기반으로 계산되었고 공지된 투입량과 관련될 수 있다. 투입-산출 상관성을 대수 공간에서 계산하였으나, 상기 세트 농도 범위가 RC-1에서 단지 10배이고 RC-2에서 102 배에 걸쳐 있기 때문에 선형 공간에서도 또한 계산할 수 있었다. 피어슨 적률상관계수, 피어슨의 r은 정확한 측정을 위해서 1에 접근해야 한다. 상기 상관성 플롯을 도 16A에 나타낸다. 상기 r-값은 샘플 RC-1에서 SIRV에 대해 0.446이고, 샘플 RC-2에서 SIRV에 대해 0.932이다(표 10을 참조하시오).
동일한 서브믹스로부터 기원하는 12 내지 21개 전사물의 동몰 농도는 유의수준 품질 척도로서 평균 및 분산을 계산할 수 있게 한다. 각각의 SIRV 믹스에 대해서 상기 서열분석 파이프라인의 품질은 상응하는 분산과 함께 4개의 상대적인 평균값의 세트로서 입증될 수 있다. 상기 검정된 파이프라인에 대한 결과는 각각 RC-1의 경우 1.21±56.05%, 0.93±46.56%, 0.97±49.46%, 및 1.02±71.62%이고, RC-2의 경우 1.56±75.75%, 0.93±54.83%, 0.94±44.46%, 및 1.02±54.48%이다. 상기 상대적인 평균은 전체 농도 범위에 걸쳐 1에 가깝지만, 높은 분산은 개별적인 SIRV가 큰 분산으로 측정됨을 입증한다.
상이한 어노테이션 SIRV_C, _I 및 _O에 대해 지도화 후 RC-1 및 RC-2에서 SIRV 중 및 상기 간의 스파이크-인 및 측정된 상대적인 농도 및 농도비의 비교. r-값을 로그-공간에서 계산하였다. 예상 및 측정된 전체 SIRV 농도를 믹스 중에 실제로 존재하는 SIRV(4열), 불충분하게 어노테이션된 SIRV(15-16열) 및 과-어노테이션된 SIRV(27-28열)에 대해 나타낸다.
1 2 3 4 5 6 7 8 9 10
2 상대적인 농도 RC -1 RC -2 RC -2/1
3 및 농도비 평균 표준편차 평균 표준편차 평균 표준편차
4 SIRV_C 69/69 1 1 　 　
5 1/64 0.02 　 　 0.04 0.07
6 1/32 0.03 　 　 0.05 0.04 　 　
7 1/4 0.25 0.30 0.17 0.23 0.13 　 　
8 1/2 0.50 0.46 0.22 0.54 0.22
9 1 1 0.97 0.48 0.94 0.42 1.00 0.16
10 2 2 2.03 1.45 　 　
11 4 4 　 　 4.09 2.23 　 　
12 8 8 　 　 　 　
13 16 16 　 　 12.44 2.92
14 r-값 0.466 0.932 0.851
15 SIRV_I 44/69 0.62 0.67 　 　
16 meas 0.77 0.81 　 　
17 1/64 0.02 　 　 0.09 1.75
18 1/32 0.03 　 　 0.10 0.12 　 　
19 1/4 0.25 0.22 0.12 0.40 0.61 　 　
20 1/2 0.50 0.34 0.22 1.09 1.42
21 1 1 0.98 0.42 0.70 0.51 0.81 0.75
22 2 2 1.45 1.17 　 　
23 4 4 　 　 2.93 1.43 　 　
24 8 8 　 　 　 　
25 16 16 　 　 13.09 0.40
26 r-값 0.407 0.813 0.889
27 SIRV_O 100/69 1.00 1.00 　 　
28 meas 1.05 1.03 　 　
29 1/64 0.02 　 　 0.03 0.89
30 1/32 0.03 　 　 0.05 0.04 　 　
31 1/4 0.25 0.30 0.16 0.23 0.12 　 　
32 1/2 0.50 0.45 0.20 0.56 0.55
33 1 1 1.00 0.57 0.97 0.49 1.02 0.28
34 2 2 2.16 1.65 　 　
35 4 4 　 　 4.18 2.07 　 　
36 8 8 　 　 　 　
37 16 16 　 　 　 　 13.07 0.32
38 r-값 0.507 0.699 0.871
가장 정확하고 재현가능한 평가는 차별적인 발현값 또는 변화배수를 측정함으로서 실현될 수 있다. 상기 믹스는 4개의 서브믹스의 정확한 부피측정 조합에 의해 제조되었기 때문에, 격차는 상기 SIRV의 완전-길이 완전성과 같은 다른 품질 척도에 의해 영향을 받지 않는다. 예상 및 측정된 변화배수간의 비교를 도 16B에 나타내며, 평균 값은 마찬가지로 표 10, 컬럼 9, 5 내지 13열에 나타낸다. 상응하는 분산과 함께 상대적인 평균값은 2.82의 오프셋 및 ±169.9%의 분산과 함께 1/64의 비에서 출발하여, 1.07±41.0%, 1.00±16.2 및 0.78±23.5%까지 계속되는 값들을 나타낸다. 상기 r-값은 0.851에 도달하였다. 비교적 큰 분산은 개별적인 SIRV의 거짓 측정, 및 NGS 파이프라인에 의한 주요한 불일치 정량분석이 유의수준의 변화를 도출하고, 따라서 올바른 정량분석에서 불확실성을 도출함을 암시한다. 큰 분산은 이미 일부 SIRV가 상기가 속하는 서브믹스의 주요 분획에 비례하지 않게 행동함을 암시한다. 4개의 상기와 같은 명백한 예를 SIRV 그룹 1 및 2에서 볼 수 있으며(표 11 참조), 다른 SIRV 그룹들에서 더 많이 볼 수 있다. 한편으로 SIRV 101, 102, 103, 106, 107, 109, 203, 204 및 205의 차별적인 유전자 발현은 상기 세트 비와 10% 미만만큼, SIRV 206의 경우는 15% 미만만큼 상이한 반면, 다른 한편으로 SIRV 105, 108 및 202의 비는 40% 초과만큼, SIRV 201의 비는 250%만큼 상이하다. 대다수 종의 비는 정확하며 4개의 상이한 서브믹스 모두에서 자명하다. 따라서, 명백한 편차는 라이브러리 생성, 서열분석 및/또는 데이터 분석에서 만들어진 오차에 의해 발생한다.
Figure pct00045
지도화를 상이한 어노테이션 SIRV_I 및 SIRV_O를 사용하여 반복하였다. 버전 SIRV_I(불충분한 어노테이션 이하)는 새로운 전사물 변이체를 검출하는 파이프라인의 능력을 판단할 수 있게 한다. 상기 실험은 어노테이션되지 않은 SIRV의 리드가 어떻게 상기 정량분석을 왜곡하는 어노테이션된 부분집합에 가짜로 분포되는지를 나타낸다. 상기 유도된 농도에서 변화 정도는 RNA-Seq 파이프라인의 확고함(robust)에 대한 추가적인 척도를 제공한다. 본 실험의 경우 상관성 플롯은 저하된다. 상기 r-값은 샘플 RC-1에서 SIRV의 경우 0.406, 및 샘플 RC-2에서 SIRV의 경우 0.813으로 떨어진다. 추가적인 오차는 균일하게 확대되는 듯하며 예상 및 측정 변화 배수간의 비교는 심지어 0.889의 약간 더 높은 r-값을 보인다.
상기 과-어노테이션된 버전 SIRV_O는 세 번째 상황을 반영한다. 여기에서, 상기 샘플들 중에 실제로 함유된 것보다 많은 SIRV가 어노테이션된다. 상기 어노테이션은 예를 들어 다른 조직, 동일한 조직이지만 상이한 발생 단계에서 발견될 수도 있거나, 거짓으로 어노테이션되었거나, 또는 보다 초기 실험에서의 잔존생물인 전사물 변이체를 포함하며, 이 경우 전형적인 길이의 클로닝된 EST를 갖는 다수의 변이체가 전형적인 예이다. 이제, 리드를 실제로 상기 실제 샘플의 부분이 아닌 SIRV 변이체로 할당할 수 있다. 본 실험의 경우 상기 상관성 플롯은 RC-1의 경우 0.506 및 RC-2의 경우 0.699의 r-값을 나타낸다. 상기 예상 및 측정 변화 배수간의 비교는 0.871의 유사한 r-값을 나타낸다.
올바른 SIRV 검출의 정도 및 확고성은 상기 파이프라인 수행성능에 대한 척도이다.
RNA-Seq 실험에서 정확성 수준의 측정을 SIRV 스파이크-인 대조군을 사용하여 상이한 방식으로 수행할 수 있다. 임의의 다른 천연 유전자와 마찬가지로, SIRV 유전자의 변이체는 특유의 증명 서열(telling sequence) 범위에서 상이한 정도에 따라 변한다. 상기 서열 독특성은 NGS 리드를 전사물 변이체로 할당시 해결해야 하는 "단순한" 및 "보다 어려운" 임무의 조합을 포함하는 유전자의 복잡성에 대한 척도이다. 어노테이션과 관련하여 하나의 전사물의 특정한 숫자는 상대적인 변이체 특이성 서열, RSS(뉴클레오티드 수준에 따라 카운트되며 그의 길이에 대해 표준화된다)이다. 각각의 전사물에 대해 공유된 뉴클레오티드 수는 경쟁하는 전사물 변이체의 수에 역비례한다. 서열 복잡성 C에 대한 척도는 전사물의 길이, L로 나눈 모든 역 RSS 값의 합이다. 측정 농도 대 스파이크-인 농도의 상대 편차 배수, D는 이제 상기 서열 복잡성에 의해 가중될 수 있다. 상기 전사물 변이체에 대한 올바른 리드 할당의 도전은 상기 어노테이션의 근원적인 복잡성에 비례한다. 상기 서열 복잡성 C를 곱한, 로그-편차 배수, D의 역 모듈러스(inverse modulus)는 하기에 따른 농도 크기의 가중된 정확성에 대한 척도, A이다:
ASIRV = f1{(Σ(1/RSS))/L}/ f2{|log2 D|} = f1{C}/ f2{|log2 D|}
상기 2개의 함수, f1 및 f2는 상이한 성분들의 증량, 및 예를 들어 상대적인 편차가 1에 접근하고, 따라서 로그가 0에 접근하며 몫이 한정되지 않는 경우 상기 측정 농도 및 스파이크-인 농도의 완벽한 조화를 허용하는 경계 조건의 한정을 허용한다. 결과적으로 상기 SIRV_O 어노테이션내의 모든 69개 SIRV의 올바른 측정은, 정확한 농도 크기를 획득하는 것은 본질적으로 보다 어렵기 때문에, 상기 SIRV_C 어노테이션내에서와 같이 보다 높은 값에 도달할 수 있다. 변화 배수는 주어진 한계와 함께 할당되어야 하는데, 그렇지 않으면 0에 가까운 값이 의미 있는 데이터 평가를 왜곡하기 때문이다.
상대적인 변이체 특이성 서열, RSS 및 복잡성, C를 하나의 예에서 SIRV1의 출발시 중복 서열을 살펴봄으로써 설명할 수 있다. SIRV107은 중복 센스 전사물인 반면 SIRV108 및 109는 중복 안티센스 전사물이다. 어노테이션 SIRV_I에서 SIRV109의 서열은 SIRV108이 생략되기 때문에 독특하며, 각 뉴클레오티드의 모든 1/RSS 값은 1이고, SIRV109의 길이를 곱하고 나누어도 상기 값은 여전히 1이다. 어노테이션 SIRV_C에서 SIRV109의 서열은 SIRV108과 그의 서열의 일부를 공유하므로 더 이상 독특하지 않다. 상응하는 1/RSS 값은 2이고, 복잡성은 >1이다. 어노테이션 SIRV_O에서 SIRV109의 서열은 오직 SIRV108과만 그의 서열의 일부, 오직 SIRV110과만 그의 서열의 일부(이때 상응하는 1/RSS 값은 다시 2이다), 및 상기 둘 다와 그의 서열의 일부(이때 상응하는 1/RSS 값은 3이다)를 공유하는 반면, 그의 서열 중 어느 것도 독특하지 않다. 여기에서, SIRV109의 C-값은 다시 보다 크다. SIRV109의 가중된 정확성, A는 상기 C 값에 비례하고 믹스 E1 및 E2의 공지된 SIRV109 투입으로부터 측정된 log2-편차 배수의 모듈러스에 역비례한다.
상기 변화 배수는 추가로 차별적인 발현의 소환시 참 및 거짓 양성률, TP 및 FP와 같은 다수의 매개변수들을 계산할 수 있게 한다. 상기 TP 대 FP 곡선 아래 면적, AUC는 차별적인 발현 분석에서 진단학적 수행성능을 위한 척도로서 간주될 수 있다.
실시예 8: 신뢰성 있는 적용을 위한 SIRV 및 다른 대조용 분액의 희석, 안정화 및 제조
RNA는 RNase 또는 2가 양이온 및 온도에 의해 가속화되는 가수분해에 의해 분해되기 쉽다. 더욱이, RNA는 다수의 표면에 의해 흡착되는 경향이 있다. 따라서, 전기영동 젤 또는 ERCC 믹스용 RNA 사다리와 같은 RNA 대조군은 산화방지제 및 첨가제, 예를 들어 EDTA, DDT, RNasin 또는 다른 RNase 억제제를 함유하는 완충제 중에 25 ng/㎕ 이상의 농도로 제공된다. 상기와 같은 RNA 용액은 전형적으로 -20 ℃의 심부 온도에서 보관된다. RNA에의 비교를 위해 낮은 백분율 범위로 상기 RNA 대조군을 사용하는 경우, 픽토그램의 수십 배의 분액이 필요하며, 고농축 대조군은 스파이크-인에 적합하기 위해 다양하게 희석되어야 한다. 단지 몇 개의 샘플을 한 번에 처리할 필요가 있는 경우, 상기 희석된 대조군의 대부분을 배치해야 한다. 분액의 희석 및 제조는 불필요한 변화를 도입시킬 위험이 있다.
본 실시예에서 SIRV를 사용하기 쉽게, 주어진 실험에 필요한 총량의 안정한 분액으로 제조한다. 상술한 E0, E1, E2 또는 SIRV 단독 또는 추가적인 RNA 대조용과의 임의의 다른 조합과 같은 SIRV 믹스를 젠테그라(GenTegra)-RNA(젠테그라), RNAstable(바이오매트리카(Biomatrica)), 또는 상기 용액을 건조시키면서 RNA의 분해를 감소시키는 다른 첨가제와 같은 안정화제를 함유하는 무-RNase 완충제를 사용하여 모액으로부터 1 pg/㎕, 10 pg/㎕ 또는 100 pg/㎕로 희석한다. 이어서, 상기 희석된 RNA 대조군을 갖는 용액을 목적하는 양의 분액으로 바이알을 통해 나눈 후에 상기 용액을 주변 온도에서 빠르게 건조시키거나 동결건조시킨다. 상기 분액을 시간 독립적으로 나중의 적용으로부터 제조하는 경우 분액의 수뿐만 아니라 부피도 비교적 클 수 있으며, 이는 상기 대조용의 제조 재현성을 증가시킨다. 상기 대조용 RNA의 건조된 분액들을 실온에서 보관할 수 있다.
상기 대조용 RNA 분액이 필요한 경우, 표적 RNA 샘플을 단지 임의의 가공 단계에서 상기 건조된 대조용 RNA에 첨가하기만 하면 된다. 수 분 정도로 짧은 배양 시간이 상기 건조된 RNA 대조군을 해결하는데 필요하다. 이러한 수단에 의해 상기 샘플은 상기 RNA 대조군에 신뢰성 있게 스파이크된다.
하나의 바람직한 예에서 상기 RNA 대조군은 바코드 서열과 같은 특유의 식별자를 갖는 RNA를 함유한다. 상기 바코드 서열은 상기 바코드 서열의 존재를 표시하는 특유의 인공 서열이 측면에 인접해 있다. 상기 대조군 중의 바코드는 상기 RNA 샘플이 상기 대조군에 첨가되는 순간부터 상기 샘플이 내부 바코드로 독특하게 식별됨을 보증한다. 상기 내부 바코드와 외부 샘플 표지의 매치는 고속대량처리 환경에서 확실히 잘못 보지 않게 한다.
임의의 서열분석 실험에서 상기 대조용 RNA 및 바코드의 존재는 상기 샘플의 추적 및 샘플 처리의 비교가능성을 보장한다.
실시예 9: 서열-특이성 연결 편향을 설명하는 micro-RNA와 같은 추가적인 스파이크-인 대조용과 SIRV와의 조합
SIRV를 ERCC, 상기 언급한 바코드 RNA, 또는 인공 micro-RNA와 같은 다른 RNA 대조군과 결합시킬 수 있다. micro-RNA는 전형적으로 21 내지 23 nt 정도로 짧은 RNA이다. 제한된 크기로 인해 micro-RNA 라이브러리 제조의 작업 흐름은, 프라이밍 및 cDNA 합성이 방해/영향을 받기 때문에 상이하다. 상기 micro-RNA는 직접적으로 직접 연결되어야 한다. 말단 서열 및 특히 소수의 개시- 및 종료-부위는 105 정도로 클 수 있는 강한 편향을 도입시키는 원인이다. 따라서, 연결 반응에서 서열 편향의 측정을 허용하는 특별한 micro-RNA 대조군이 필요하다.
여기에서, 우리는, 우세하게는 21 내지 23 nt 길이이나 16 nt만큼 짧고 36 nt만큼 길 수 있는 서열의 종료뿐만 아니라 개시에서 4, 5 및 8 이하 랜덤 뉴클레오티드, N(8)의 랜덤 서열을 갖는 인공 micro-RNA를 사용한다. 상기 인공 micro-RNA는 합성된다. 본 발명에서 주된 장애물은 A, U, G 및 C의 혼합물이 또한 임의의 합성 편향을 보상하는데 사용되고 miRNA 합성 실행시 작은 변화가 뉴클레오티드 분포에 현저한 변화를 유도할 수 있으며, 이는 차례로 편향을 평가하는데 사용되기 때문에 엄격하게 조절되어야 한다는 것이다. 따라서, 상기 인공 micro-RNA는 또한 중간 부분에 몇몇 랜덤 뉴클레오티드, N을 적어도 하나 내지, 개시-부위의 N과 종료-부위의 N 사이의 최대의 N의 수로 또한 함유한다.
상기 중간의 N은 상기 뉴클레오티드의 분포에서 무작위성의 독립적인 척도를 제공하는 반면, N의 신장부에서 개시- 및 종료-부위의 N은 상기 micro-RNA 라이브러리 제조의 서열 편향을 측정할 수 있게 한다.
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Figure pct00069
Figure pct00070
SEQUENCE LISTING <110> Lexogen GmbH <120> Methods and products for quantifying RNA transcript variants <130> IPA161585-AT <150> EP14176417.5 <151> 2014-07-09 <160> 345 <170> BiSSAP 1.3 <210> 1 <211> 1591 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 1 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acagctatcc 240 tgaggtgcca ggttagaata ggccctacca catctaagcc ttgaaacggt ctgttcctcg 300 tgtcaagttc tttgtccgct tataccgcca tctctcgttg tggtacaaga gttggttact 360 ctcagtccca ttcccaacac atcactgaga agtatagagc ctagtggtca ttagaaggtc 420 aacatggagt aggtgtttag gtgtgtcgga gggcagaaac caacgaaaga aaggaaacga 480 tcaacactta tatagtgtcc tgtcgggccc aaatcctaag agaccttagg tttaccacat 540 tagtactaaa cagcctgttg tataccgtga gcccccactt cgatctgtaa atggtaatcc 600 cttgtaaaac ctcatctcca aaaacagtat acgcaggtac agctgatgtg atcctctcta 660 ctagtcttct ctgaggaacc atgagatgga tagtggggga cctgagtcat actagggtaa 720 cttcttatat ggtgacatca ctcctaccgt agagggggga cggtagtctg tgggaaatct 780 accctagtga agaaaggtaa gagaatgtct gaacattacc tgtagacgaa ggtactctca 840 gaacaactac tctcacatac ggaaaggttt gggtccttgg tcaaaggact ttactgactc 900 tgcagacgat gaatatctga accaacacta ctatcaaaga tgttcgacta gacctctcgt 960 ggtagggcct ggaagtcaaa agacttctct atgggtacta ggtaggtagt tagagtacgg 1020 tccctggggt gtctcagagt cgaacgttcg ggggaagttc ctaaacatgt taggatgtta 1080 tgataacaag tccaaacgtg gacgtcgtcg ctgtcacctc tcggaacact agctgaggtg 1140 gcttaaaaac attccgtaac tgagtggtca ccgggggtga aacatgctga gggttctacc 1200 ttaacgggaa ggtgaaactc tccaatgtct ttgaaaggac tagttgagat aacccaaacc 1260 gaatccggag gaagaggaga acttggaaaa cgttgtacga cgtcattaat aacgattccg 1320 ttcttcccgt tcgtgtctat aaggatattc tttcattgcg aaggtaagcc cttttgggtc 1380 tagatacgga ctatactcaa aaacccacag accttccact tggagttgtt ttaggagacg 1440 gagtagtaga gaaaacttgt ggcaccttcc cagtcggttc cctacagact ctccttagta 1500 gctcctaaga tcgtcgagtt acacgacgac attgttccga ctgacgtaga ggcattactt 1560 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 1591 <210> 2 <211> 1330 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 2 gtacaagagt tggttactct cagtcccatt cccaacacat cactgagaag tatagagcct 60 agtggtcatt agaaggtcaa catggagtag gtgtttaggt gtgtcggagg gcagaaacca 120 acgaaagaaa ggaaacgatc aacacttata tagtgtcctg tcgggcccaa atcctaagag 180 accttaggtt taccacatta gtactaaaca gcctgttgta taccgtgagc ccccacttcg 240 atctgtaaat ggtaatccct tgtaaaacct catctccaaa aacagtatac gcaggtacag 300 ctgatgtgat cctctctact agtcttctct gaggaaccat gagatggata gtgggggacc 360 tgagtcatac tagggtaact tcttatatgg tgacatcact cctaccgtag aggggggacg 420 gtagtctgtg ggaaatctac cctagtgaag aaaggtaaga gaatgtctga acattacctg 480 tagacgaagg tactctcaga acaactactc tcacatacgg aaaggtttgg gtccttggtc 540 aaaggacttt actgactctg cagacgatga atatctgaac caacactact atcaaagatg 600 tcgtaggttt gtgagaaggg agaagaagtg gttgagaagt tggtgttcca caagttaaag 660 tagacatacc tttagtacct gtaaaggttc gactagacct ctcgtggtag ggcctggaag 720 tcaaaagact tctctatggg tactaggtag gtagttagag tacggtccct ggggtgtctc 780 agagtcgaac gttcggggga agttcctaaa catgttagga tgttatgata acaagtccaa 840 acgtggacgt cgtcgctgtc acctctcgga acactagctg aggtggctta aaaacattcc 900 gtaactgagt ggtcaccggg ggtgaaacat gctgagggtt ctaccttaac gggaaggtga 960 aactctccaa tgtctttgaa aggactagtt gagataaccc aaaccgaatc cggaggaaga 1020 ggagaacttg gaaaacgttg tacgacgtca ttaataacga ttccgttctt cccgttcgtg 1080 tctataagga tattctttca ttgcgaaggt aagccctttt gggtctagat acggactata 1140 ctcaaaaacc cacagacctt ccacttggag ttgttttagg agacggagta gtagagaaaa 1200 cttgtggcac cttcccagtc ggttccctac agactctcct tagtagctcc taagatcgtc 1260 gagttacacg acgacattgt tccgactgac gtagaggcat aaaaaaaaaa aaaaaaaaaa 1320 aaaaaaaaaa 1330 <210> 3 <211> 1393 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 3 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 120 agataaaaat aacaacaaca gaaggtacaa gagttggtta ctctcagtcc cattcccaac 180 acatcactga gaagtataga gcctagtggt cattagaagg tcaacatgga gtaggtgttt 240 aggtgtgtcg gagggcagaa accaacgaaa gaaaggaaac gatcaacact tatatagtgt 300 cctgtcgggc ccaaatccta agagacctta ggtttaccac attagtacta aacagcctgt 360 tgtataccgt gagcccccac ttcgatctgt aaatggtaat cccttgtaaa acctcatctc 420 caaaaacagt atacgcaggt acagctgatg tgatcctctc tactagtctt ctctgaggaa 480 ccatgagatg gatagtgggg gacctgagtc atactagggt aacttcttat atggtgacat 540 cactcctacc gtagaggggg gacggtagtc tgtgggaaat ctaccctagt gaagaaaggt 600 aagagaatgt ctgaacatta cctgtagacg aaggtactct cagaacaact actctcacat 660 acggaaaggt ttgggtcctt ggtcaaagga ctttactgac tctgcagacg atgaatatct 720 gaaccaacac tactatcaaa gatgttcgac tagacctctc gtggtagggc ctggaagtca 780 aaagacttct ctatgggtac taggtaggta gttagagtac ggtccctggg gtgtctcaga 840 gtcgaacgtt cgggggaagt tcctaaacat gttaggatgt tatgataaca agtccaaacg 900 tggacgtcgt cgctgtcacc tctcggaaca ctagctgagg tggcttaaaa acattccgta 960 actgagtggt caccgggggt gaaacatgct gagggttcta ccttaacggg aaggtgaaac 1020 tctccaatgt ctttgaaagg actagttgag ataacccaaa ccgaatccgg aggaagagga 1080 gaacttggaa aacgttgtac gacgtcatta ataacgattc cgttcttccc gttcgtgtct 1140 ataaggatat tctttcattg cgaaggtaag cccttttggg tctagatacg gactatactc 1200 aaaaacccac agaccttcca cttggagttg ttttaggaga cggagtagta gagaaaactt 1260 gtggcacctt cccagtcggt tccctacaga ctctccttag tagctcctaa gatcgtcgag 1320 ttacacgacg acattgttcc gactgacgta gaggcattac tttaaaaaaa aaaaaaaaaa 1380 aaaaaaaaaa aaa 1393 <210> 4 <211> 1429 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 4 gttttacaaa ggagaacttc caacatcagt gtatgggatg tttcaaacac ccccagagac 60 ggagacaata gtgtggtcac gtacatttag gaagataaaa ataacaacaa cagaagcctt 120 gaaacggtct gttcctcgtg tcaagttctt tgtccgctta taccgccatc tctcgttgtg 180 gtacaagagt tggttactct cagtcccatt cccaacacat cactgagaag tatagagcct 240 agtggtcatt agaaggtcaa catggagtag gtgtttaggt gtgtcggagg gcagaaacca 300 acgaaagaaa ggaaacgatc aacacttata tagtgtcctg tcgggcccaa atcctaagag 360 accttaggtt taccacatta gtactaaaca gcctgttgta taccgtgagc ccccacttcg 420 atctgtaaat ggtaatccct tgtaaaacct catctccaaa aacagtatac gcaggtacag 480 ctgatgtgat cctctctact agtcttctct gaggaaccat gagatggata gtgggggacc 540 tgagtcatac tagggtaact tcttatatgg tgacatcact cctaccgtag aggggggacg 600 gtagtctgtg ggaaatctac cctagtgaag aaaggtaaga gaatgtctga acattacctg 660 tagacgaagg tactctcaga acaactactc tcacatacgg aaaggtttgg gtccttggtc 720 aaaggacttt actgactctg cagacgatga atatctgaac caacactact atcaaagatg 780 ttcgactaga cctctcgtgg tagggcctgg aagtcaaaag acttctctat gggtactagg 840 taggtagtta gagtacggtc cctggggtgt ctcagagtcg aacgttcggg ggaagttcct 900 aaacatgtta ggatgttatg ataacaagtc caaacgtgga cgtcgtcgct gtcacctctc 960 ggaacactag ctgaggtggc ttaaaaacat tccgtaactg agtggtcacc gggggtgaaa 1020 catgctgagg gttctacctt aacgggaagg tgaaactctc caatgtcttt gaaaggacta 1080 gttgagataa cccaaaccga atccggagga agaggagaac ttggaaaacg ttgtacgacg 1140 tcattaataa cgattccgtt cttcccgttc gtgtctataa ggatattctt tcattgcgaa 1200 ggtaagccct tttgggtcta gatacggact atactcaaaa acccacagac cttccacttg 1260 gagttgtttt aggagacgga gtagtagaga aaacttgtgg caccttccca gtcggttccc 1320 tacagactct ccttagtagc tcctaagatc gtcgagttac acgacgacat tgttccgact 1380 gacgtagagg cattacttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 1429 <210> 5 <211> 700 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 5 gcgcaaggta catgctctta ccccactctc agcacgtagg gagcggagta caagagttgg 60 ttactctcag tcccattccc aacacatcac tgagaagtat agagcctagt ggtcattaga 120 aggtcaacat ggagtaggtg tttaggtgtg tcggagggca gaaaccaacg aaagaaagga 180 aacgatcaac acttatatag tgtcctgtcg ggcccaaatc ctaagagacc ttaggtttac 240 cacattagta ctaaacagcc tgttgtatac cgtgagcccc cacttcgatc tgtaaatggt 300 aatcccttgt aaaacctcat ctccaaaaac agtatacgca ggtacagctg atgtgatcct 360 ctctactagt cttctctgag gaaccatgag atggatagtg ggggacctga gtcatactag 420 ggtaacttct tatatggtga catcactcct accgtagagg ggggacggta gtctgtggga 480 aatctaccct agtgaagaaa ggtaagagaa tgtctgaaca ttacctgtag acgaaggtac 540 tctcagaaca actactctca catacggaaa ggtttgggtc cttggtcaaa ggactttact 600 gactctgcag acgatgaata tctgaaccaa cactactatc aaagatgttc gactagacct 660 ctcgtggtag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 700 <210> 6 <211> 1003 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 6 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acagtgttta 240 ggtgtgtcgg agggcagaaa ccaacgaaag aaaggaaacg atcaacactt atatagtgtc 300 ctgtcgggcc caaatcctaa gagaccttag gtttaccaca ttagtactaa acagcctgtt 360 gtataccgtg agcccccact tcgatctgta aatggtaatc ccttgtaaaa cctcatctcc 420 aaaaacagta tacgcaggta cagctgatgt gatcctctct actagtcttc tctgaggaac 480 catgagatgt tatgataaca agtccaaacg tggacgtcgt cgctgtcacc tctcggaaca 540 ctagctgagg tggcttaaaa acattccgta actgagtggt caccgggggt gaaacatgct 600 gagggttcta ccttaacggg aaggtgaaac tctccaatgt ctttgaaagg actagttgag 660 ataacccaaa ccgaatccgg aggaagagga gaacttggaa aacgttgtac gacgtcatta 720 ataacgattc cgttcttccc gttcgtgtct ataaggatat tctttcattg cgaaggtaag 780 cccttttggg tctagatacg gactatactc aaaaacccac agaccttcca cttggagttg 840 ttttaggaga cggagtagta gagaaaactt gtggcacctt cccagtcggt tccctacaga 900 ctctccttag tagctcctaa gatcgtcgag ttacacgacg acattgttcc gactgacgta 960 gaggcattac tttaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 1003 <210> 7 <211> 774 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 7 gtgaggcatc tataccgcca cgccctatgt ctttctcacg tataggtgtg ctctcagctg 60 ccgttccggc gctgcttcac ccctttccgc ttatcgcgga tggtaaagtc aaagtttact 120 cagcggtcgc tgttgacctc aggttctcta tcaaaaattt gctggctaaa acggtcggag 180 cttctgctcc actctttcca gcaatccttc tgccagtcgc cttcaaactt gcaatctgtg 240 gattcgtcga acgtccgggt agtatagtta tctgcatcgt ggtcaccagt atcacagact 300 acaaaatctc aatcctccat tcccccgtca ggtctatcta aaattcaatc gctgtgtttg 360 cggggtaaat aataggacaa gtccgtataa tctacccaat cgcattcatt ccagtcagag 420 ggtaccaccg aatttcatca acaacattaa taattccata ggaactacat tcttaaatcc 480 ttggaactcc aaactcctac cttatcacgg ttctatccgt cgtcttaggg tcggtctcct 540 cgtagcatgg cctcgttctc ctcgccgtaa ttgcggtcga aagtacttca gttatttatg 600 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 660 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 720 agataaaaat aacaacaaca gaagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 774 <210> 8 <211> 732 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 8 gtcttccaca ctccgctccc tacgtgctga gagtggggta agagcatgta ccttgcgcct 60 cacaccttct gttgttgtta tttttatctt cctaaatgta cgtgaccaca ctattgtctc 120 cgtctctggg ggtgtttgaa acatcccata cactgatgtt ggaagttctc ctttgtaaaa 180 cccggttgac actcgcggac aagcctcact actttcgacc gcaattacgg cgaggagaac 240 gaggccatgc tacgaggaga ccgaccctaa gacgacggat agaaccgtga taaggtagga 300 gtttggagtt ccaaggattt aagaatgtag ttcctatgga attattaatg ttgttgatga 360 aattcggtgg taccctctga ctggaatgaa tgcgattggg tagattatac ggacttgtcc 420 tattatttac cccgcaaaca cagcgattga attttagata gacctgacgg gggaatggag 480 gattgagatt ttgtagtctc acagattgca agtttgaagg cgactggcag aaggattgct 540 ggaaagagtg gagcagaagc tccgaccgtt ttagccagca aatttttgat agagaacctg 600 aggtcaacag cgaccgctga gtaaactttg actttaccat ccgcgataag cggaaagggg 660 tgaagcagcg ccggaacggc agctgagagc acacctatac gtaaaaaaaa aaaaaaaaaa 720 aaaaaaaaaa aa 732 <210> 9 <211> 494 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 9 gggtgtttga aacatcccat acactgatgt tggaagttct cctttgtaaa acccggttga 60 cactcgcgga caagcctcac cataaataac tgaagtactt tcgaccgcaa ttacggcgag 120 gagaacgagg ccatgctacg aggagaccga ccctaagacg acggatagaa ccgtgataag 180 gtaggagttt ggagttccaa ggatttaaga atgtagttcc tatggaatta ttaatgttgt 240 tgatgaaatt cggtgaagga ttgctggaaa gagtggagca gaagctccga ccgttttagc 300 cagcaaattt ttgatagaga acctgaggtc aacagcgacc gctgagtaaa ctttgacttt 360 accatccgcg ataagcggaa aggggtgaag cagcgccgga acggcagctg agagcacacc 420 tatacgtgag aaagacatag ggcgtggcgg tatagatgcc tcacaaaaaa aaaaaaaaaa 480 aaaaaaaaaa aaaa 494 <210> 10 <211> 2081 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 10 gtgtatttat ccgctacgtg gtggatgaac atggatgcga acgacatttc gaaatatacg 60 caatgatatc caaccctacc gaaacagcaa taattgtaga aggtagcata tcatgccgag 120 agtttactac gttgtagcga gtttagttga aatagtttgt gatgtgtaaa ctaaaacata 180 ccaaatggta aaacttgtca cccccattta cattgcgaca gacacagtta tggagtggat 240 ggaagtgtgt tcgactgtca agacccttct ataatagtcg aaccagtacg gtaagtgggc 300 aacatgccca tataagtcga agcatttcaa atggttcatc cagtagacga cgatgtccta 360 gacagtcatg tctcttcccc atttgaagtc atgtagtggt tgtctacttt ttcattgaag 420 acgtccacct caacgctatg aacgttagtt gagacaatgt taaagcaaac gacaacgtca 480 ttgtgatcta catacacagt ggatggttag cgtaaacatg gtggaacgta ctttgactgc 540 gctgcaagaa atggttgggt cgatcgtaat gctagtcgtt acatcggaac aagccaaaac 600 aaatcattcg ctggatttag acctactgca cgacgacgtc gacacaagac attcttgaaa 660 ggaattcaat gcagaaactc ctatgcacgt cgaactagtc tcttgctcag cgctagtcca 720 tgaaaatgtc ctagctttga acgtttatca aatgcaagtt gtgggagtta gtcatgtgag 780 agttgtgcaa gaaagtgtta acgttctagc cgtgctcagt aacaattacc attcggcggt 840 aacaaacgta agttgtcact gcactcatga gcatgaagtt agaccagtag ttgggtcaga 900 aattagacgt ggtttagtct ggttaccgtg gaaagaatag ctgaactcaa agaagccgac 960 aaagataaac aaagaagaaa cggttaatac gattgaggag aaatattcaa tccaacggca 1020 actattaacg tttcaactag aatctacgtt tcatacgcgg attccacatc cgttcttgca 1080 attaaagaaa cgatgtagaa aacgctttgg ttggcgacaa cttgggcggc aaaataatta 1140 tggtaggtaa ttgaagtagc gttaaaacgc aaaaagaagg aagcacggat cactgcaaaa 1200 aggactactc aataatggtt tctaaagtca ttatgctggt gctccctatg gcggtggtca 1260 gtactgtaac taaagatgga ttacggcact tgtagagcac gtatcagacc cagctttgaa 1320 agaaggctga gttcggttcg ggtttcacgt aggcctagaa cgaggtatag aagtggttaa 1380 caaagatttg gttaccatgt aagaagcggt agacgccatg gcctagtagg aagaatgctt 1440 gttcacggag ccgacgaagt tagacgagtg gcagaagaaa gagcattatc ttggtactcg 1500 gactgaatgc gtactacggt cgtccaagtt gcaagaggta ttagagtcat tcgaatagct 1560 gtgggagtgg tatttatagc ataataagtg taaaaaacat tcccaacagc atatgtgtag 1620 ccaagaagcg ttgtgctcgc gactaaaagt caggtagcag caggcattaa cccagtgata 1680 gaacatcaaa gtcaaataag tatggtttag cattactgcg tatcttgcga ttgcgtgctg 1740 gtggtcaaca gtgcaaacct agtggcttcg cactcaaatg tagacaagca ttaaccaact 1800 acttctaacg tatgttcagg aagaatggct attctcgtca ggtgaatgcg taatcgaatg 1860 agtagtatgg tcgaacgtcc cgctatagtc tgacacgatt caggcaaagt tggagtgcaa 1920 agccatgatt tggtcgatgc ggcaaatgtt gcttaggact attctatggt cttgcgtaat 1980 tgtgaagatt tgccagttat ctaaggagac gtggtgattc gctctatagc tgtatctaat 2040 ctgccagatt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 2081 <210> 11 <211> 2001 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 11 gttggtgtat ttatccgcta cgtggtggat gaacatggat gcgaacgaca tttcgaaata 60 tacgcaatga tatccaaccc taccgaaaca gcaataattg tagaaggtag catatcatgc 120 cgagagttta ctacgttgta gcgagtttag ttgaaatagt ttgtgatgtg taaactaaaa 180 cataccaaat ggtaaaactt gtcaccccca tttacattgc gacagacaca gttatggagt 240 ggatggaagt gtgttcgact gtcaagaccc ttctataata gtcgaaccag tacggtaagt 300 gggcaacatg cccatataag tcgaagcatt tcaaatggtt catccagtag acgacgatgt 360 cctagacagt catgtctctt ccccatttga agtcatgtag tggttgtcta ctttttcatt 420 gaagacgtcc acctcaacgc tatgaacgtt agttgagaca atgttaaagc aaacgacaac 480 gtcattgtga tctacataca cagtggatgg ttagcgtaaa catggtggaa cgtactttga 540 ctgcgctgca agaaatggtt gggtcgatcg taatgctagt cgttacatcg gaacaagcca 600 aaacaaatca ttcgctggat ttagacctac tgcacgacga cgtcgacaca agacattctt 660 gaaagcgcta gtccatgaaa atgtcctagc tttgaacgtt tatcaaatgc aagttgtggg 720 agttagtcat gtgagagttg tgcaagaaag tgttaacgtt ctagccgtgc tcagtaacaa 780 ttaccattcg gcggtaacaa acgtaagttg tcactgcact catgagcatg aagttagacc 840 agtagttggg tcagaaatta gacgtggttt agtctggtta ccgtggaaag aatagctgaa 900 ctcaaagaag ccgacaaaga taaacaaaga agaaacggtt aatacgattg aggagaaata 960 ttcaatccaa cggcaactat taacgtttca actagaatct acgtttcata cgcggattcc 1020 acatccgttc ttgcaattaa agaaacgatg tagaaaacgc tttggttggc gacaacttgg 1080 gcggcaaaat aattatggta ggtaattgaa gtagcgttaa aacgcaaaaa gaaggaagca 1140 cggatcactg caaaaaggac tactcaataa tggtttctaa agtcattatg ctggtgctcc 1200 ctatggcggt ggtcagtact gtaactaaag atggattacg gcacttgtag agcacgtatc 1260 agacccagct ttgaaagaag gctgagttcg gttcgggttt cacgtaggcc tagaacgagg 1320 tatagaagtg gttaacaaag atttggttac catgtaagaa gcggtagacg ccatggccta 1380 gtaggaagaa tgcttgttca cggagccgac gaagttagac gagtggcaga agaaagagca 1440 ttatcttggt actcggactg aatgcgtact acggtcgtcc aagttgcaag aggtattaga 1500 gtcattcgaa tagctgtggg agtggtattt atagcataat aagtgtaaaa aacattccca 1560 acagcatatg tgtagccaag aagcgttgtg ctcgcgacta aaagtcaggt agcagcaggc 1620 attaacccag tgatagaaca tcaaagtcaa ataagtatgg tttagcatta ctgcgtatct 1680 tgcgattgcg tgctggtggt caacagtgca aacctagtgg cttcgcactc aaatgtagac 1740 aagcattaac caactacttc taacgtatgt tcaggaagaa tggctattct cgtcaggtga 1800 atgcgtaatc gaatgagtag tatggtcgaa cgtcccgcta tagtctgaca cgattcaggc 1860 aaagttggag tgcaaagcca tgatttggtc gatgcggcaa atgttgctta ggactattct 1920 atggtcttgc gtaattgtga agatttgcca gttatctaag gagacgtggt gaaaaaaaaa 1980 aaaaaaaaaa aaaaaaaaaa a 2001 <210> 12 <211> 716 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 12 gctacgtggt ggatgaacat ggatgcgaac gacatttcga aatatacgca atgatatcca 60 accctaccga aacagcaata attgtagaag gtagcatatc atgccgagtc atcgacatgc 120 ctttatcgac acacgacgtg attggagttt actacgttgt agcgagttta gttgaaatag 180 tttgtgatgt gtaaactaaa acataccaaa tggtaaaact tgtcaccccc atttacattg 240 cgacagacac agttatggag tggatggaag tgtgttcgac tgtcaagacc cttctataat 300 agtcgaacca gtacggtaag tgggcaacat gcccatataa gtcgaagcat ttcaaatggt 360 tcatccagta gacgacgatg tcctagacag tcatgtctct tccccatttg aagtcatgta 420 gtggttgtct actttttcat tgaagacgtc cacctcaacg ctatgaacgt tagttgagac 480 aatgttaaag caaacgacaa cgtcattgtg atctacatac acagtggatg gttagcgtaa 540 acatggtgga acgtactttg actgcgctgc aagaaatggt tgggtcgatc gtaatgctag 600 tcgttacatc ggaacaagcc aaaacaaatc attcgctgga tttagaccta ctgcacgacg 660 acgtcgacac aagacattct tgaaagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 716 <210> 13 <211> 770 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 13 gtaaaacttg tcacccccat ttacattgcg acagacacag ttatggagtg gatggaagtg 60 tgttcgactg tcaagaccct tctataatag tcgaaccagt acggtaagtg ggcaacatgc 120 ccatataagt cgaagcattt caaatggttc atccagtaga cgacgatgtc ctagacagtc 180 atgtctgtca catctatcac gcctcctacg ttgtgccata acgaagaata atagtgataa 240 gagacatcga agctgtcgta gtcgttcgct aaattgctta caatttagtg ctctaagtga 300 acaatgtttc atcttgagaa gcaaacgaag tagaagttga cgacgcattc gaagacgtgg 360 acaatgtgac acgagtatcg aaacatgatc caacgaacat cgaagaagtt actactgaac 420 tacgggtcag cttccccatt tgaagtcatg tagtggttgt ctactttttc attgaagacg 480 tccacctcaa cgctatgaac gttagttgag acaatgttaa agcaaacgac aacgtcattg 540 tgatctacat acacagtgga tggttagcgt aaacatggtg gaacgtactt tgactgcgct 600 gcaagaaatg gttgggtcga tcgtaatgct agtcgttaca tcggaacaag ccaaaacaaa 660 tcattcgctg gatttagacc tactgcacga cgacgtcgac acaagacatt cttgaaaggt 720 aattgacgtg gacgtttcag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 770 <210> 14 <211> 553 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 14 gccgcatcga ccaaatcatg gctttgcact ccaactttgc ctgaatcgtg tcagactata 60 gcgggacgtt cgaccatact actcattcga ttacgcattc acctgacgag aatagccatt 120 cttcctgaac atacgttaga agtagttggt taatgcttgt ctacatttga gtgcgaagcc 180 actaggtttg cactgttgac caccagcacg caatcgcaag atacgcagta atgctaaacc 240 atacttattt gactttgatg ttctatcact gggttaatgc ctgctgctac ctgactttta 300 gtcgcgagca caacgcttct tggctacaca tatgctgttg ggaatgtttt ttacacttat 360 tatgctataa ataccactcc cacagctatt cgaatgactc taatacctct tgcaacttgg 420 acgaccgtag tacgcattca gtccgagtac caagataatg ctctttcttc tgccactcgt 480 ctaacttcgt cggctccgtg aacaagcatt cttcctacta ggcaaaaaaa aaaaaaaaaa 540 aaaaaaaaaa aaa 553 <210> 15 <211> 454 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 15 gttgaggtgg acgtcttcaa tgaaaaagta gacaaccact acatgacttc aaatggggaa 60 gctgacccgt agttcagtag taacttcttc gatgttcgtt ggatcatgtt tcgatactcg 120 tgtcacattg tccacgtctt cgaatgcgtc gtcaacttct acttcgtttg cttctcaaga 180 tgaaacattg ttcacttaga gcactaaatt gtaagcaatt tagcgaacga ctacgacagc 240 ttcgatgtct cttatcacta ttattcttcg ttatggcaca acgtaggagg cgtgatagat 300 gtgacagaca tgactgtcta ggacatcgtc gtctactgga tgaaccattt gaaatgcttc 360 gacttatatg ggcatgttgc ccacttaccg tactggttcg actattatag aagggtcttg 420 acagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 454 <210> 16 <211> 2497 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 16 gtggagaagc aaatacttgg ataccgaatt tagaggccat aggttatgga aaaagtcagt 60 ggattagata gtaaaagtcg aaatctttat attcggttcg atgtactacg ttattcgcga 120 ctgttctgtc atgatagttt tttatatagt cctaataaaa ggagttttta aagtaaatga 180 catttcagta gtaaaagttt aagagctttt tgttctgcca gacgttctga tagttgagga 240 aaacgtgtgg tcagctaata acttgtggta tggagtaaac gaacatccaa cagtatgtac 300 aatcgtatga gaaaggacaa atctgaagac gatattagag ggagtccaac aagtttgtga 360 tgattcacgt aaggaaatcg aaatgtaaga ttattcgtca atggaggtat aagacaacac 420 accaagtaat tatacgcgac gagttttcat gttgacgatc cctgagacta tatcatacaa 480 aattgaccca aataataaac tcgttccaat agttgaagtg ttgattaaca cattgagaca 540 agttgtccgt actttaatca tcaaagtttt ggactgacct ataaatgaaa tattcgcgtt 600 gtcttcagtt taagaagtga tattattttc ggtttcaagc ctatgtggag agatacagga 660 aataggtaaa ctataactaa tataaatata cgcagacgaa gcctgttaac tttaccaaat 720 ccaaatagcg ggagacacta aacttacaag aaatgtacgg taagacaggg aaacagggtt 780 tgaagtttgg aggtacaaaa tcttaatcct tcacaactga atcaacattt acattcggtt 840 acgttcgggg tttgtagtag taattgttga ggtaatttta attgcactta cagacgtgga 900 tttggtgaaa cattatgtgg tagattcata tttccgaatc ttaatagacg attttatgat 960 ggtgatcgtg gtttcttttc aaggtttggt ggacgaaaat gaaaaaatca caatttaacg 1020 tttcatattc atcacaatcc gaaatcgaga tggagagaaa gtttgactaa actttgttac 1080 agtatttttg aaggcaatca acctggaact ttggatttca tcaagtaaac acccatattt 1140 agcgaaaatg caagctaact gcgaactaac aaggtaactc tttttcgatt tcaaaatatg 1200 gtcagtagca atatgttaag tgatccattt tggaggggtg gggatttgtt caatgaatga 1260 ggcaataaac caccgcaacc tctgtaattg acgagaaact tgtataaggt catcaaaaag 1320 aatagatgaa cataaagatt gaatacggct gggtgaggtt aattcagatt tcccaaatta 1380 tgtagatgaa aagatggtaa gtttattgat aggtaaaaaa tcaagagagg gtaataaaga 1440 agaaagaata atcaaaagat agagcatgca aacattacat ggtagtattg ttataggcgg 1500 tatattggta aatgacgatc cttgacttag ttttttacat cgaacggttg caaatcctag 1560 catacttgat attaatagta gatactgaaa tatatctgag tatatcaagg gaaatcgaga 1620 tgtaacaata agtcgaggaa attaaggtca atttagtgct tatcatagaa ataactactt 1680 aagcccttaa cgaactaact caaattaacg aggtagaggt tattctgaat tgtacgaacg 1740 tagtcgaagt ttgttaccaa gggtaaagcc aaaacaaaac gatttgacaa gagaatctat 1800 tccatgtagt agtgcgtttg tacgacgttc tattcgatgt aaatcattag gacgagggtt 1860 tgaagcaact cggaaagatg gtcggttaga atttaaaagt actgaacgga attaacgaag 1920 ttggctacta cctaatagtc tttgcttagg atttagtagt tcgcaattgg gtcaaagtgg 1980 tcgctttggt cgtcgacgtc aagactcaac aagtcattta ccgctgtacg aaccatctca 2040 tttaacaggt tctcaaccaa atagcggtag gagtagaaag cacttaccat cgtacgaact 2100 ttgacaacga aaggttcaaa ggtaaccaag gcgtagtagt gagttaggtc aatgtataaa 2160 aactcccttt cttattttga gtcgtcaatg tagtcgagaa agtatgcact tagtgccatt 2220 attattagac aagatagtcc gttctttagt cgaagttctt gttgtaataa caaaagtaga 2280 gcagattacg atccgaaatt tcatggacac agatatttga gatcaacatt tgattcgttg 2340 acgtatttat gttgattcgg ttagcgagag ttacaaagtt agtaatagaa agaaagagga 2400 aataataatc aaaagattaa gcatgcaaac attaagagga cacatatgta ttggtggcat 2460 ttgcgttaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 2497 <210> 17 <211> 1837 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 17 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggagtaaac gaacatccaa 60 cagtatgtac aatcgtatga gaaaggacaa atctgaagac gatattagag ggagtccaac 120 aagtttgtga tgattcacgt aaggaaatcg aaatgtaaga ttattcgtca atggaggtat 180 aagacaacac accaagtaat tatacgcgac gagttttcat gttgacgatc cctgagacta 240 tatcatacaa aattgaccca aataataaac tcgttccaat agttgaagtg ttgattaaca 300 cattgagaca agttgtccgt actttaatca tcaaagtttt ggactgacct ataaatgaaa 360 tattcgcgtt gtcttcagtt taagaagtga tattattttc ggtttcaagc ctatgtggag 420 agatacagga aataggtaaa ctataactaa tataaatata cgcagacgaa gcctgttaac 480 tttaccaaat ccaaatagcg ggagacacta aacttacaag aaatgtacgg taagacaggg 540 aaacagggtt tgaagtttgg aggtacaaaa tcttaatcct tcacaactga atcaacattt 600 acattcggtt acgttcgggg tttgtagtag taattgttga ggtaatttta attgcactta 660 cagacgtgga tttggtgaaa cattatgtgg tagattcata tttccgaatc ttaatagacg 720 attttatgat ggtgatcgtg gtttcttttc aaggtttggt ggacgaaaat gaaaaaatca 780 caatttaacg tttcatattc atcacaatcc gaaatcgaga tggagagaaa gtttgactaa 840 actttgttac agtatttttg aaggcaatca acctggaact ttggatttca tcaagtaaac 900 acccatattt agcgaaaatg caagctaact gcgaactaac aaggtaactc tttttcgatt 960 tcaaaatatg gtcagtagca atatgttaag tgatccattt tggaggggtg gggatttgtt 1020 caatgaatga ggcaataaac caccgcaacc tctgtaattg acgagaaact tgtataaggt 1080 catcaaaaag aatagatgaa cataaagatt gaatacggct gggtgaggtt aattcagatt 1140 tcccaaatta tgtagatgaa aagatggtaa gtttattgat aggtaaaaaa tcaagagagg 1200 gtaataaaga agaaagaata atcaaaagat agagcatgca aacattacat ggtagtattg 1260 ttataggcgg tatattggta aatgacgatc cttgacttag ttttttacat cgaacggttg 1320 caaatcctag catacttgat attaatagta gatactgaaa tatatctgag tatatcaagg 1380 gaaatcgaga tgtaacaata agtcgaggaa attaaggtca atttagtgct tatcatagaa 1440 ataactactt aagcccttaa cgaactaact caaattaacg aggtagaggt tattctgaat 1500 tgtacgaacg tagtcgaagt ttgttaccaa gggtaaagcc aaaacaaaac gatttgacaa 1560 gagaatctat tccatgtagt agtgcgtttg tacgacgttc tattcgatgt aaatcattag 1620 gacgagggtt tgaagcaact cggaaagatg gtcggttaga atttaaaagt actgaacgga 1680 attaacgaag ttggctacta cctaatagtc tttgcttagg atttagtagt tcgcaattgg 1740 gtcaaagtgg tcgctttggt cgtcgacgtc aagactcaac aagtcattta ccgctgtacg 1800 aaccatcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 1837 <210> 18 <211> 2048 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 18 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggattagat agtaaaagtc 60 gaaatcttta tattcggttc gatgtactac gttattcgcg actgttctgt catgatagtt 120 ttttatatag tcctaataaa aggagttttt aaagtaaatg acatttcagt agtaaaagtt 180 taagagcttt ttgttctgcc agacgttctg atagttgagg aaaacgtgtg gtcagctaat 240 aacttgtggt atggagtaaa cgaacatcca acagtatgta caatcgtatg agaaaggaca 300 aatctgaaga cgatattaga gggagtccaa caagtttgtg atgattcacg taaggaaatc 360 gaaatgtaag attattcgtc aatggaggta taagacaaca caccaagtaa ttatacgcga 420 cgagttttca tgttgacgat ccctgagact atatcataca aaattgaccc aaataataaa 480 ctcgttccaa tagttgaagt gttgattaac acattgagac aagttgtccg tactttaatc 540 atcaaagttt tggactgacc tataaatgaa atattcgcgt tgtcttcagt ttaagaagtg 600 atattatttt cggtttcaag cctatgtgga gagatacagg aaataggtaa actataacta 660 atataaatat acgcagacga agcctgttaa ctttaccaaa tccaaatagc gggagacact 720 aaacttacaa gaaatgtacg gtaagacagg gaaacagggt ttgaagtttg gaggtacaaa 780 atcttaatcc ttcacaactg aatcaacatt tacattcggt tacgttcggg gtttgtagta 840 gtaattgttg aggtaatttt aattgcactt acagacgtgg atttggtgaa acattatgtg 900 gtagattcat atttccgaat cttaatagac gattttatga tggtgatcgt ggtttctttt 960 caaggtttgg tggacgaaaa tgaaaaaatc acaatttaac gtttcatatt catcacaatc 1020 cgaaatcgag atggagagaa agtttgacta aactttgtta cagtattttt gaaggcaatc 1080 aacctggaac tttggatttc atcaagtaaa cacccatatt tagcgaaaat gcaagctaac 1140 tgcgaactaa caaggtaact ctttttcgat ttcaaaatat ggtcagtagc aatatgttaa 1200 gtgatccatt ttggaggggt ggggatttgt tcaatgaatg aggcaataaa ccaccgcaac 1260 ctctgtaatt gacgagaaac ttgtataagg tcatcaaaaa gaatagatga acataaagat 1320 tgaatacggc tgggtgaggt taattcagat ttcccaaatt atgtagatga aaagatggta 1380 agtttattga taggtaaaaa atcaagagag ggtaataaag aagaaagaat aatcaaaaga 1440 tagagcatgc aaacattaca tggtagtatt gttataggcg gtatattggt aaatgacgat 1500 ccttgactta gttttttaca tcgaacggtt gcaaatccta gcatacttga tattaatagt 1560 agatactgaa atatatctga gtatatcaag ggaaatcgag atgtaacaat aagtcgagga 1620 aattaaggtc aatttagtgc ttatcataga aataactact taagccctta acgaactaac 1680 tcaaattaac gaggtagagg ttattctgaa ttgtacgaac gtagtcgaag tttgttacca 1740 agggtaaagc caaaacaaaa cgatttgaca agagaatcta ttccatgtag tagtgcgttt 1800 gtacgacgtt ctattcgatg taaatcatta ggacgagggt ttgaagcaac tcggaaagat 1860 ggtcggttag aatttaaaag tactgaacgg aattaacgaa gttggctact acctaatagt 1920 ctttgcttag gatttagtag ttcgcaattg ggtcaaagtg gtcgctttgg tcgtcgacgt 1980 caagactcaa caagtcattt accgctgtac gaaccatcaa aaaaaaaaaa aaaaaaaaaa 2040 aaaaaaaa 2048 <210> 19 <211> 1113 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 19 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggtacgaat tacgaacacc 60 ttaacattag ggaaacttag atagttgtac aaagtaacgt ataacaagta tcgcgtatgg 120 attagatagt aaaagtcgaa atctttatat tcggttcgat gtactacgtt attcgcgact 180 gttctgtcat gatagttttt tatatagtcc taataaaagg agtttttaaa gtaaatgaca 240 tttcagtagt aaaagtttaa gagctttttg ttctgccaga cgttctgata gttgaggaaa 300 acgtgtggtc agctaataac ttgtggtatg gagtaaacga acatccaaca gtatgtacaa 360 tcgtatgaga aaggacaaat ctgaagacga tattagaggg agtccaacaa gtttgtgatg 420 attcacgtaa ggaaatcgaa atgtaagatt attcgtcaat ggaggtataa gacaacacac 480 caagtaatta tacgcgacga gttttcatgt tgacgatccc tgagactata tcatacaaaa 540 ttgacccaaa taataaactc gttccaatag ttgaagtgtt gattaacaca ttgagacaag 600 ttgtcctatt gttataggcg gtatattggt aaatgacgat ccttgactta gttttttaca 660 tcgaacggtt gcaaatccta gcatacttga tattaatagt agcatcgtac gaactttgac 720 aacgaaaggt tcaaaggtaa ccaaggcgta gtagtgagtt aggtcaatgt ataaaaactc 780 cctttcttat tttgagtcgt caatgtagtc gagaaagtaa gatgcactta gtgccattat 840 tattagacaa gatagtccgt tctttagtcg aagttcttgt tgtaataaca aaagtagagc 900 agattacgat ccgaaatttc atggacacag atatttgaga tcaacatttg attcgttgac 960 gtatttatgt tgattcggtt agcgagagtt acaaagttag taatagaaag aaagaggaaa 1020 taataatcaa aagattaagc atgcaaacat taagaggaca catatgtatt ggtggcattt 1080 gcgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 1113 <210> 20 <211> 466 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 20 gtacgaatta cgaacacctt aacattaggg aaacttagat agttgtacaa agtaacgtat 60 aacaagtatc gcgtatggat tagatagtaa aagtcgaaat ctttatattc ggttcgatgt 120 actacgttat tcgcgactgt tctgtcatga tagtttttta tatagtccta ataaaaggag 180 tttttaaagt aaatgacatt tcagtagtaa aagtttaaga gctttttgtt ctgccagacg 240 ttctgatagt tgaggaaaac gtgtggtcag ctaataactt gtggtatgtt tggaggtaca 300 aaatcttaat ccttcacaac tgaatcaaca tttacattcg gttacgttcg gggtttgtag 360 tagtaattgt tgaggtaatt ttaattgcac ttacagacgt ggatttggtg aaacattatg 420 tggtagattc atatttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 466 <210> 21 <211> 2403 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 21 gtggagaagc aaatacttgg ataccgaatt tagaggccat aggttatgga aaaagtcagt 60 ggtacgaatt acgaacacct taacattagg gaaacttaga tagttgtaca aagtaacgta 120 taacaagtat cgcgtatgga gtaaacgaac atccaacagt atgtacaatc gtatgagaaa 180 ggacaaatct gaagacgata ttagagggag tccaacaagt ttgtgatgat tcacgtaagg 240 aaatcgaaat gtaagattat tcgtcaatgg aggtataaga caacacacca agtaattata 300 cgcgacgagt tttcatgttg acgatccctg agactatatc atacaaaatt gacccaaata 360 ataaactcgt tccaatagtt gaagtgttga ttaacacatt gagacaagtt gtccgtactt 420 taatcatcaa agttttggac tgacctataa atgaaatatt cgcgttgtct tcagtttaag 480 aagtgatatt attttcggtt tcaagcctat gtggagagat acaggaaata ggtaaactat 540 aactaatata aatatacgca gacgaagcct gttaacttta ccaaatccaa atagcgggag 600 acactaaact tacaagaaat gtacggtaag acagggaaac agggtttgaa gtttggaggt 660 acaaaatctt aatccttcac aactgaatca acatttacat tcggttacgt tcggggtttg 720 tagtagtaat tgttgaggta attttaattg cacttacaga cgtggatttg gtgaaacatt 780 atgtggtaga ttcatatttc cgaatcttaa tagacgattt tatgatggtg atcgtggttt 840 cttttcaagg tttggtggac gaaaatgaaa aaatcacaat ttaacgtttc atattcatca 900 caatccgaaa tcgagatgga gagaaagttt gactaaactt tgttacagta tttttgaagg 960 caatcaacct ggaactttgg atttcatcaa gtaaacaccc atatttagcg aaaatgcaag 1020 ctaactgcga actaacaagg taactctttt tcgatttcaa aatatggtca gtagcaatat 1080 gttaagtgat ccattttgga ggggtgggga tttgttcaat gaatgaggca ataaaccacc 1140 gcaacctctg taattgacga gaaacttgta taaggtcatc aaaaagaata gatgaacata 1200 aagattgaat acggctgggt gaggttaatt cagatttccc aaattatgta gatgaaaaga 1260 tggtaagttt attgataggt aaaaaatcaa gagagggtaa taaagaagaa agaataatca 1320 aaagatagag catgcaaaca ttacatggta gtattgttat aggcggtata ttggtaaatg 1380 acgatccttg acttagtttt ttacatcgaa cggttgcaaa tcctagcata cttgatatta 1440 atagtagata ctgaaatata tctgagtata tcaagggaaa tcgagatgta acaataagtc 1500 gaggaaatta aggtcaattt agtgcttatc atagaaataa ctacttaagc ccttaacgaa 1560 ctaactcaaa ttaacgaggt agaggttatt ctgaattgta cgaacgtagt cgaagtttgt 1620 taccaagggt aaagccaaaa caaaacgatt tgacaagaga atctattcca tgtagtagtg 1680 cgtttgtacg acgttctatt cgatgtaaat cattaggacg agggtttgaa gcaactcgga 1740 aagatggtcg gttagaattt aaaagtactg aacggaatta acgaagttgg ctactaccta 1800 atagtctttg cttaggattt agtagttcgc aattgggtca aagtggtcgc tttggtcgtc 1860 gacgtcaaga ctcaacaagt catttaccgc tgtacgaacc atctcattta acaggttctc 1920 aaccaaatag cggtaggagt agaaagcact taccatcgta cgaactttga caacgaaagg 1980 ttcaaaggta accaaggcgt agtagtgagt taggtcaatg tataaaaact ccctttctta 2040 ttttgagtcg tcaatgtagt cgagaaagtg tccaagcaaa acgtaacgaa acggttgagt 2100 aaggacttgc acactatgcc cttgcgggtt tacctgcttg acgtagtcct gccacttatt 2160 atccgtgatt gttacctaat ggacaaccta atcgttagct taaagaagat gcacttagtg 2220 ccattattat tagacaagat agtccgttct ttagtcgaag ttcttgttgt aataacaaaa 2280 gtagagcagt attttgacgt atagggagac agtcattagg tcattaaccc agttactaag 2340 gacaacgtaa ccatcgaagt tattgaactt cagaaaaaaa aaaaaaaaaa aaaaaaaaaa 2400 aaa 2403 <210> 22 <211> 809 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 22 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggtacgaat tacgaacacc 60 ttaacattag ggaaacttag atagttgtac aaagtaacgt ataacaagta tcgcgtatga 120 tagtaaaagt cgaaatcttt atattcggtt cgatgtacta cgttattcgc gactgttctg 180 tcatgatagt tttttatata gtcctaataa aaggagtttt taaagtaaat gacatttcag 240 tagtaaaagt ttaagagctt tttgttctgc cagacgttct gatagttgag gaaaacgtgt 300 ggtcagctaa taacttgtgg agtaaacgaa catccaacag tatgtacaat cgtatgagaa 360 aggacaaatc tgaagacgat attagaggga gtccaacaag tttgtgatga ttcacgtaag 420 gaaatcgaaa tgtaagatta ttcgtcaatg gaggtataag acaacacacc aagtaattat 480 acgcgacgag ttttcatgtt gacgatccct gagactatat catacaaaat tgacccaaat 540 aataaactcg ttccaatagt tgaagtgttg attaacacat tgagacaagt tgtccgatta 600 cgatccgaaa tttcatggac acagatattt gagatcaaca tttgattcgt tgacgtattt 660 atgttgattc ggttagcgag agttacaaag ttagtaatag aaagaaagag gaaataataa 720 tcaaaagatt aagcatgcaa acattaagag gacacatatg tattggtggc atttgcgtta 780 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 809 <210> 23 <211> 509 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 23 ggcctctaaa ttcggtatcc aagtatttgc ttctccacct gccaagcgca cataaattct 60 ttgcgagtgt tgtttggcca cttttggtag ctcctgtttc ttggcaattt tggctgacac 120 gttcagtttc ttttgctcca acttcgtaag cagttagtgt aggcgtgcgc taaggcattg 180 cgctactcgc ttccatcgtt ggttctacca cccatatgcc atggcgtcct gtaagtttgc 240 gcctaattac tcgaagcgct ttcatttcta cgaaacgttt tggaataatg tcaacttggg 300 cattgttgaa actacggctt ccttaatgct cttgacaacc ttgtttagtt gttgttcttc 360 cttcttccag catttaataa ccggcttttg ttttaactcc ttcgcttttt cgactttctc 420 ctttagcacc gcaacgttct ttttaagcta caacgctttc ttgaaatgct tctcgtcata 480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 509 <210> 24 <211> 826 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 24 gtattagggc gcattgcgct ttgccgaacg cgtaattaca gaacttgtgg tttcggtttt 60 gtcgatagcg actggaactg ccgaactttg tttaataatt acacgttaat ctgggacaaa 120 aggcaagctg aagatcgcag taacagttgg ttctgcgaaa gttgaccaac ctatgcgact 180 ttctggtttt gcctgttatg aacaacgttg gcaggtaaag aagcggctga ctgctggtcg 240 aagagccaaa tggtcaacat tagcatagct tgtttcgtaa tcgattggct caactgccgc 300 cgtgtccacg cgttaaacgc ggttagtaac atccactgaa ctttcttcgc caacagtaca 360 agctggcagt tgcatgtctt tagtacagga gattgcaacg ctacctacgc aagctttgac 420 tgcgatgcta caccgcacga tagctcgcgt acctacactt ttacgcgcta ctactccgcg 480 aacataagcc acttcatgtt gaacgaatgg agaacgttat ggttcacttc tttatgcgac 540 cgaagaaggc gcagtgtttg cttgacaatt tactgtcgtg tcttcggtaa cagatatgct 600 accaagcgac ctaaccactt taagcagctt caccgtctta cgagacgtta acctaacgca 660 aatgccacca atacatatgt gtcctcttaa tgtttgcatg cttaatcttt tgattattat 720 ttcctctttc tttctattac taactttgta actctcgcta accgaatcaa cataaatacg 780 tcaacgaatc aaatgtaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 826 <210> 25 <211> 619 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 25 gcgtaattac agaacttgtg gtttcggttt tgtcgatagc gactggaact gccgaacttt 60 gtttaataat tacacgttaa tctgggacaa aaggcaagct gaagatcgca gtaacagttg 120 gttctgcgaa agttgaccaa cctatgcgac tttctggttt tgcctgttat gaacaacgtt 180 ggcaggtaaa gaagcggctg actgctggtc gaagagccaa atggtcaaca ttagcatagc 240 ttgtttcctc tttttcgttg cggagttaag taggagaacg ttatggttca cttctttatg 300 cgaccgaaga aggcgcagtg tttgcttgac aatttactgt cgtgtcttcg gtaacagata 360 tgctaccaag cgacctaacc accttcaccg tcttacgaga cgttaaccta acgcaaatgc 420 caccaataca tatgtgtcct cttaatgttt gcatgcttaa tcttttgatt attatttcct 480 ctttctttct attactaact ttgtaactct cgctaaccga atcaacataa atacgtcaac 540 gaatcaaatg ttgatctcaa atatctgtgt ccatgaaatt tcggatcgta aaaaaaaaaa 600 aaaaaaaaaa aaaaaaaaa 619 <210> 26 <211> 191 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 26 gctgaccaca cgttttcctc aactatcaga acgtctggca gaacaaaaag ctcttaaact 60 tttactactg aaatgtcatt tactttaaaa actcctttta ttaggactat ataaaaaact 120 atcatgacag aacagtcgcg aataacgtag tacatcgaac caaaaaaaaa aaaaaaaaaa 180 aaaaaaaaaa a 191 <210> 27 <211> 2283 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 27 gttgaactta tacagaaata ctaaaagtcg aacaaccatt cgatttagga atacagaacg 60 ataacgcagc agaagtagtt gaccaccgtg taacttgaat ggtgatggtc ctggttctat 120 taatacaaat ccttttactt atgctccgtg atacagaaca actaaaagaa ctaaaagaga 180 agaacgacgt gcacgtgcta attatgcgaa ttaacatcgg gttagaaaaa gtaaactaaa 240 ggaaaatggg taaggcatat gccttactaa attgtgtaaa agaagttcta ataagaaccg 300 gaatgtatgt aattattcgc aagggttcca taaggcacat atacaaattt agaaacaacc 360 cgttacatta acaaaggtga acggtgtttc acagatatta gaccattagg taaccaatga 420 catttcacta tcgaaaaggg tatagtatta actactacaa ctaatccgac tcgtcgaggt 480 gatttataca ggactgcagg tagcaaacga cgaggtggac gagggtaagg tggccgacga 540 aagggaggac atgcacctag ttgaacacga ctaggaggac gatggccact acggagtgga 600 acacaaaggt aaagttgcaa taataaccag tacaacaaaa gcatacgtgg aaggtgacaa 660 gcaaacgatg tcttgaacaa caccgtcttc attgtttacc atttgctgtt ggatttgata 720 tttagagcgt atgttgaaga agttggaact ctggacacat tagagactta ttggatcgat 780 caagaggaat gcaatacgaa gtagtttcac tttcgtaggt taccctataa cacgcaaata 840 gatgtaatca acctcttatt aatcatacac tagacatcag atgttaatga cctaacgatt 900 atagtgctga acaacgtcta cgacaatata aacctttgca aagactggtc aaacatcaat 960 gttaaacacg tccagataaa agcaaaaagg taagacgagg attacggaat aattaatata 1020 gtgcttgttc caatcggccc ggcttcagtc attcccggtg gcgaagccat aacatttaaa 1080 aatagcctgt taacttcaag ctgtagtagc ctaaacaggt ttcctaatag tatgcaaaca 1140 acatagttca atagaagtta ttaataaaca tcaaattgtc tttgaacaac acaaacacgt 1200 aagctttcgg caactaaatt aactaaaaaa taactataat tacacagaac acgaagtcat 1260 ctgtttccta ttaaaaaata ggtacttgac aggttacaat ataacctgtt tctagcatgt 1320 ataacaaaat taagcgacga ccgagcgacg acaacaagca gcagccaggt gggcaaaagg 1380 ctaagactgc tttgatgtcc tgagccaact tgccgatacg cagcaacatt ttagaaacgt 1440 catcctgacc gccgccgcca ttgccataaa tgatagcttg gcggtagccg cccaaaacta 1500 tgaaaaaaat taaattaagg aaagacataa acaaggtagt taagccatgc acctagaaaa 1560 ttttgaacgg ctcagctgca acatattagc gctagaaatg acctaccata atgatataga 1620 agataattac cattactgcc accgcctcct ccgctgccgc ctcctccata gcagcttcta 1680 ttcaaacaaa ctccgccgcc gccaccgccg ccataaccac caccataacc accgccgtat 1740 acacaaacgc cgctccttct aagtcttagc tattaataac aaccgtggaa acaaaaaacg 1800 taatataggc tacagctgtg tcaacagcca aatccataac aacaaaatcc atggtgactg 1860 taacagacac tgttacaacc atattattaa ctagatagtg gttacagata atcatgcagc 1920 aacaacatat aaagaagacg gttaaagtta ttgacttatg ttagcatgtg caaattaaca 1980 taggctagtc ttaacctgac tcgctgtcgc gactggcatg caaagtttga cacgccgcgg 2040 ctctagtacg cgtcatcttt cagctgtaat aactacaaac acatcaaaaa aagtcggttt 2100 aaaacaactt gtgaaacgtc aacttggtaa ataaacctat tattaaagaa tctagatcaa 2160 tgtccaaaaa gattatttcc attgttacgt ctaaatacag attctgcgaa aacaagccat 2220 tagtatagta gaagaactaa ccatcgtatg ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa 2280 aaa 2283 <210> 28 <211> 2089 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 28 gtgtaaccat cttacggatg taatctaacg taaacaccag agaaacaacc atcaaaacgg 60 cataatgggc aagcattatt aggaacatct aaatataaaa ccattgcctt aactcacata 120 acgctacact tggtaacacc accatatgca gtgcaagaac gttgggtaag agaacatcga 180 acgtatggtc tgtttacata agctcaaaaa taattttaca gggtaaaacg tcaatcatta 240 cttaacagca gtaatgctta caaatgtttc ggttagtatt taaaaccgta acgaacgcca 300 cgtgtaaaaa caagaggttt tattaatatt gggtatatta gatgtatccg tcgattaacc 360 caccataacc aaactaaatt aaagcaacga cttatgcgaa atcctccttg tttacaaact 420 aacagtaatg caagtcgtaa taaacgtctt aaatatttta taggtaaaag atttggacca 480 tatcctcagt actgtaatga taaacaccga cgaagtctac atcgttgcaa cggaaaattc 540 catgaaagtc gtatgtttta tggtaaactt ccgaatttag caaaaagggt tgtaaaaggt 600 atacgtcatc atggtaagaa atacagctgt attagtatat aaggttgtag aaaaccacat 660 aataatatgt ctttagcact agacatcaga tgttaatgac ctaacgatta tagtgctgaa 720 caacgtctac gacaatataa acctttgcaa agactggtca aacatcaatg ttaaacacgt 780 ccagataaaa gcaaaaaggt aagacgagga ttacggaata attaatatag tgcttgttcc 840 aatcggcccg gcttcagtca ttcccggtgg cgaagccata acatttaaaa atagcctgtt 900 aacttcaagc tgtagtagcc taaacaggtt tcctaatagt atgcaaacaa catagttcaa 960 tagaagttat taataaacat caaattgtct ttgaacaaca caaacacgta agctttcggc 1020 aactaaatta actaaaaaat aactataatt acacagaaca cgaagtcatc tgtttcctat 1080 taaaaaatag gtacttgaca ggttacaata taacctgttt ctagcatgta taacaaaatt 1140 aagcgacgac cgagcgacga caacaagcag cagccaggtg ggcaaaaggc taagactgct 1200 ttgatgtcct gagccaactt gccgatacgc agcaacattt tagaaacgtc atcctgaccg 1260 ccgccgccat tgccataaat gatagcttgg cggtagccgc ccaaaactat gaaaaaaatt 1320 aaattaagga aagacataaa caaggtagtt aagccatgca cctagaaaat tttgaacggc 1380 tcagctgcaa catattagcg ctagaaatga cctaccataa tgatatagaa gataattacc 1440 attactgcca ccgcctcctc cgctgccgcc tcctccatag cagcttctat tcaaacaaac 1500 tccgccgccg ccaccgccgc cataaccacc accataacca ccgccgtata cacaaacgcc 1560 gctccttcta agtcttagct attaataaca accgtggaaa caaaaaacgt aatataggct 1620 acagctgtgt caacagccaa atccataaca acaaaatcca tggtgactgt aacagacact 1680 gttacaacca tattattaac tagatagtgg ttacagataa tcatgcagca acaacatata 1740 aagaagacgg ttaaagttat tgacttatgt tagcatgtgc aaattaacat aggctagtct 1800 taacctgact cgctgtcgcg actggcatgc aaagtttgac acgccgcggc tctagtacgc 1860 gtcatctttc agctgtaata actacaaaca catcaaaaaa agtcggttta aaacaacttg 1920 tgaaacgtca acttggtaaa taaacctatt attaaagaat ctagatcaat gtccaaaaag 1980 attatttcca ttgttacgtc taaatacaga ttctgcgaaa acaagccatt agtatagtag 2040 aagaactaac catcgtatga aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 2089 <210> 29 <211> 700 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 29 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggagtgcaga tacagaacaa 120 ctaaaagaac taaaagagaa gaacgacgtg cacgtgctaa ttatgcgaat taacatcggg 180 ttagaaaaag taaactaaag gaaaatgggt aaggcatatg ccttactaaa ttgtgtaaaa 240 gaagttctaa taagaaccgg aatgtatgta attattcgca agggttccat aaggcacata 300 tacaaattta gaaacaaccc gttacattaa caaaggtgaa cggtgtttca cagatattag 360 accattaggt aaccaatgac atttcactat cgaaaagggt atagtattaa ctactacaac 420 taatccgact cgtcgaggtg atttatacag gactgcaggt agcaaacgac gaggtggacg 480 agggtaaggt ggccgacgaa agggaggaca tgcacctagt tgaacacgac taggaggacg 540 atggccacta cggagtggaa cacaaaggta aagttgcaat aataaccagt acaacaaaag 600 catacgtgga aggtgacaag caaacgatgt cttgaacaac accgtcttca ttgtttacca 660 tttgctgttg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 700 <210> 30 <211> 622 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 30 gcaattagga tcaggtggtt aatctgatac gatacagaac aactaaaaga actaaaagag 60 aagaacgacg tgcacgtgct aattatgcga attaacatcg ggttagaaaa agtaaactaa 120 aggaaaatgg gtaaggcata tgccttacta aattgtgtaa aagaagttct aataagaacc 180 ggaatgtatg taattattcg caagggttcc ataaggcaca tatacaaatt tagaaacaac 240 ccgttacatt aacaaaggtg aacggtgttt cacagatatt agaccattag gtaaccaatg 300 acatttcact atcgaaaagg gtatagtatt aactactaca actaatccga ctcgtcgagg 360 tgatttatac aggactgcag gtagcaaacg acgaggtgga cgagggtaag gtggccgacg 420 aaagggagga catgcaccta gttgaacacg actaggagga cgatggccac tacggagtgg 480 aacacaaagg taaagttgca ataataacca gtacaacaaa agcatacgtg gaaggtgaca 540 agcaaacgat gtcttgaaca acaccgtctt cattgtttac catttgctgt tgaaaaaaaa 600 aaaaaaaaaa aaaaaaaaaa aa 622 <210> 31 <211> 656 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 31 gccaaggtga accaggacga agtcgatgtt tcagaacctc ctttaggcct tttagacggt 60 ttaaacgaag cactagacca agtaataaaa gcggacgttg cacaggtacg tgcagataca 120 gaacaactaa aagaactaaa agagaagaac gacgtgcacg tgctaattat gcgaattaac 180 atcgggttag aaaaagtaaa ctaaaggaaa atgggtaagg catatgcctt actaaattgt 240 gtaaaagaag ttctaataag aaccggaatg tatgtaatta ttcgcaaggg ttccataagg 300 cacatataca aatttagaaa caacccgtta cattaacaaa ggtgaacggt gtttcacaga 360 tattagacca ttaggtaacc aatgacattt cactatcgaa aagggtatag tattaactac 420 tacaactaat ccgactcgtc gaggtgattt atacaggact gcaggtagca aacgacgagg 480 tggacgaggg taaggtggcc gacgaaaggg aggacatgca cctagttgaa cacgactagg 540 aggacgatgg ccactacgga gtggaacaca aaggtaaagt tgcaataata accagtacaa 600 caaaagcata cgtggaaggt gacaagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 656 <210> 32 <211> 647 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 32 gacatcagat gttaatgacc taacgattat agtgctgaac aacgtctacg acaatataaa 60 cctttgcaaa gactggtcaa acatcaatgt taaacacgtc cagataaaag caaaaaggta 120 agacgaggat tacggaataa ttaatatagt gcttgttcca atcggcccgg cttcagtcat 180 tcccggtggc gaagccatgt caccaataaa aactatcggc ggcaatggaa gtggtttttc 240 ggaaactgtt acgggtgcaa gcggccccag tgcagtttgt gccttgccaa tcggcccagc 300 tttcaaacgc aaagttggac gacttattgg cccattctga caacgtcctg ttaaactgcc 360 cggacgttca aacggcggta tcggacgact aacgcgttac ggcggacaac tcgcccccag 420 tcaacaactt ggacacggta aatttgaacg ctaagtcaac tgtagcagtt aaacattgag 480 tattaaaata gatttaagct agcgttaaga acattaaaaa ctaaccagcc aaaccaagga 540 ttacggctgt ggtgtaatcg attgcgaaat agcatgacaa aaaacttaca gtttagaagg 600 tggcggtatt acttaagaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 647 <210> 33 <211> 2135 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 33 gcagatacag aacaactaaa agaactaaaa gagaagaacg acgtgcacgt gctaattatg 60 cgaattaaca tcgggttaga aaaagtaaac taaaggaaaa tgggtaaggc atatgcctta 120 ctaaattgtg taaaagaagt tctaataaga accggaatgt atgtaattat tcgcaagggt 180 tccataaggc acatatacaa atttagaaac aacccgttac attaacaaag gtgaacggtg 240 tttcacagat attagaccat taggtaacca atgacatttc actatcgaaa agggtatagt 300 attaactact acaactaatc cgactcgtcg aggtgattta tacaggactg caggtagcaa 360 acgacgaggt ggacgagggt aaggtggccg acgaaaggga ggacatgcac ctagttgaac 420 acgactagga ggacgatggc cactacggag tggaacacaa aggtaaagtt gcaataataa 480 ccagtacaac aaaagcatac gtggaaggtg acaagcaaac gatgtcttga acaacaccgt 540 cttcattgtt taccatttgc tgttggattt gatatttaga gcgtatgttg aagaagttgg 600 aactctggac acattagaga cttattggat cgatcaagag gaatgcaata cgaagtagtt 660 tcactttcgt aggttaccct ataacacgca aatagatgta atcaacctct tattaatcat 720 acactagaca tcagatgtta atgacctaac gattatagtg ctgaacaacg tctacgacaa 780 tataaacctt tgcaaagact ggtcaaacat caatgttaaa cacgtccaga taaaagcaaa 840 aaggtaagac gaggattacg gaataattaa tatagtgctt gttccaatcg gcccggcttc 900 agtcattccc ggtggcgaag ccataacatt taaaaatagc ctgttaactt caagctgtag 960 tagcctaaac aggtttccta atagtatgca aacaacatag ttcaatagaa gttattaata 1020 aacatcaaat tgtctttgaa caacacaaac acgtaagctt tcggcaacta aattaactaa 1080 aaaataacta taattacaca gaacacgaag tcatctgttt cctattaaaa aataggtact 1140 tgacaggtta caatataacc tgtttctagc atgtataaca aaattaagcg acgaccgagc 1200 gacgacaaca agcagcagcc aggtgggcaa aaggctaaga ctgctttgat gtcctgagcc 1260 aacttgccga tacgcagcaa cattttagaa acgtcatcct gaccgccgcc gccattgcca 1320 taaatgatag cttggcggta gccgcccaaa actatgaaaa aaattaaatt aaggaaagac 1380 ataaacaagg tagttaagcc atgcacctag aaaattttga acggctcagc tgcaacatat 1440 tagcgctaga aatgacctac cataatgata tagaagataa ttaccattac tgccaccgcc 1500 tcctccgctg ccgcctcctc catagcagct tctattcaaa caaactccgc cgccgccacc 1560 gccgccataa ccaccaccat aaccaccgcc gtatacacaa acgccgctcc ttctaagtct 1620 tagctattaa taacaaccgt ggaaacaaaa aacgtaatat aggctacagc tgtgtcaaca 1680 gccaaatcca taacaacaaa atccatggtg actgtaacag acactgttac aaccatatta 1740 ttaactagat agtggttaca gataatcatg cagcaacaac atataaagaa gacggttaaa 1800 gttattgact tatgttagca tgtgcaaatt aacataggct agtcttaacc tgactcgctg 1860 tcgcgactgg catgcaaagt ttgacacgcc gcggctctag tacgcgtcat ctttcagctg 1920 taataactac aaacacatca aaaaaagtcg gtttaaaaca acttgtgaaa cgtcaacttg 1980 gtaaataaac ctattattaa agaatctaga tcaatgtcca aaaagattat ttccattgtt 2040 acgtctaaat acagattctg cgaaaacaag ccattagtat agtagaagaa ctaaccatcg 2100 tatgtaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 2135 <210> 34 <211> 600 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 34 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggagtgcaga tacagaacaa 120 ctaaaagaac taaaagagaa gaacgacgtg cacgtgctaa ttatgcgaat taacatcggg 180 ttagaaaaag taaactaaag gaaaatgggt aaggcatatg ccttactaaa ttgtgtaaaa 240 gaagttctaa taagaaccgg aatgtatgta attattcgca agggttccat aaggcacata 300 tacaaattta gaaacaaccc gttacattaa caaaggtgaa cggtgtttca cagatattag 360 accattaggt aaccaatgac atttcactat cgaaaagggt ataggaggac atgcacctag 420 ttgaacacga ctaggaggac gatggccact acggagtgga acacaaaggt aaagttgcaa 480 taataaccag tacaacaaaa gcatacgtgg aaggtgacaa gcaaacgatg tcttgaacaa 540 caccgtcttc attgtttacc atttgctgtt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 600 <210> 35 <211> 1597 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 35 gtgtagcata gcaactgttc tgaatgtgat attcggtcag gccacgttga cacttaccaa 60 agcaagacag aaggaagttg acgggacaat agggaagatt ggcaaaggga tttaaacata 120 gtccagttcg cgtggtgaat gacagattgc tactgttacc attggcgata ctacgttggc 180 gactttactt gcctcggtag cgacagaaca agtgtcgtct acgcaacgaa caaccagcgt 240 gttagctgca atgtcccctg tagaagaccc tcttctttct cttctgaatg atgtagctgt 300 ggatagagcg tctcccacgt tagggtctag caaccctccg gtggtgtgtg gtttcggttc 360 gcttggttgg gtgcccaatt tccagtaccg aagaggtaat tgcaacttca attgcttagt 420 tgacgtgacc tttcgggtgt catggactac cgacggtatc tcatacgtga cctcgttgtt 480 gacctcgttc ttcatctgca cagactacaa cgttaggact acggaaccag tgtgaagttg 540 acggacgcgc tagaagtctg acactgacca tgcttgtggc aatcgttgtt catgtagcca 600 ctttgactcg acgttcacga acatacgatg gttagttctt ctactatact aatggcttgt 660 tttcgcagaa tctgtattta gacgtaacaa tggaaataat ctttttggac attgatctag 720 attctttaat aataggttta tttaccaagt tgacgtttca caagttgttt taaaccgact 780 ttttttgatg tgtttgtagt tattacagct gaaagatgac gcgtactaga gccgcggcgt 840 gtcaaacttt gcatgccagt cgcgacagcg agtcaggtta agactagcct atgttaattt 900 gcacatgcta acataagtca ataactttaa ccgtcttctt tatatgttgt tgctgcatga 960 ttatctgtaa ccactatcta gttaataata tggttgtaac agtgtctgtt acagtcacca 1020 tggattttgt tgttatggat ttggctgttg acacagctgt agcctatatt acgttttttg 1080 tttccacggt tgttattaat agctaagact tagaaggagc ggcgtttgtg tatacggcgg 1140 tggttatggt ggtggttatg gcggcggtgg cggcggcgga gtttgtttga atagaagctg 1200 ctatggagga ggcggcagcg gaggaggcgg tggcagtaat ggtaattatc ttctatatca 1260 ttatggtagg tcatttctag cgctaatatg ttgcagctga gccgttcaaa attttctagg 1320 tgcatggctt aactaccttg tttatgtctt tccttaattt aatttttttc atagttttgg 1380 gcggctaccg ccaagctatc atttatggca atggcggcgg cggtcaggat gacgtttcta 1440 aaatgttgct gcgtatcggc aagttggctc aggacatcaa agcagtctta gccttttgcc 1500 cacctggctg ctgcttgttg tcgtcgctcg gtcgtcgctt aattttgtta tacatgctag 1560 aaacaggaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 1597 <210> 36 <211> 980 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 36 gatcgtggcc cagtcagtgg attacgttga ccatgagtct ggctgttctt ggttcaccat 60 tagctgtggt gacaataacg ggcattgtgg caacgatacg tcgtactgca agtcccactg 120 taactaagac aattcggatt cgaccgatac ttggtctggt tcgttaactt cgcgtacctt 180 ctgctttacc agcaggtcgt caacgacgag ccaccgtaac gagtgtggtt tcggttcgct 240 tggttgggtg cccaatttcc agtaccgaag aggtaattgc aacttcaatt gcttagttga 300 cgtgaccttt cgggtgtcat ggactaccga cggtatctca tacgtgacct cgttgttgac 360 ctcgttcttc atctgcacag actacaacgt taggactacg gaaccagtgt gaagttgacg 420 gacgcgctag tttacagatg cggtttattg gattggttac aacggcaaag gcggagacca 480 ctccaactgt cggaagagta cctcttcaaa ttgccattcc aattgctcgt catggacttt 540 ccactcttag acgacccgat gaagctagaa gtctgacact gaccatgctt gtggcaatcg 600 ttgttcatgt agccactttg actcgacgtt cacgaacata cgatggttag ttcttctact 660 atactaatgg cttgttttcg cagaatctgt atttagacgt aacaatggaa ataatctttt 720 tggacattga tctagattct ttaataatag gtttatttac caagttgacg tttcacaagt 780 tgttttaaac cgactttttt tgatgtgttt gtagttatta cagctgaaag atgacgcgta 840 ctagagccgc ggcgtgtcaa actttgcatg ccagtcgcga cagcgagtca ggttaagact 900 agcctatgtt aatttgcaca tgctaacata agtcaataac tttaaccgtc aaaaaaaaaa 960 aaaaaaaaaa aaaaaaaaaa 980 <210> 37 <211> 1920 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 37 gtagaccttt ccttgccgcg tggaacaaac cgccatgagt gttttacgtt tacggtcggc 60 gaccggagtc acagcgcgac caacggggcc aggcaaaggc ccataggctt ttccatcggc 120 acgcgaccga ttccatcaag tgttaggtgc aatccccatt cgggacggac acgcgagtaa 180 aggtctaaca tgtttcaact ttccagttag tcgccgtcct atattggcaa gaccggatgg 240 ccgtcaacca gttccattct gactatttgc cacattgatt aaagaagtag gagcgttagt 300 cggcgcgtgc gcgggactat ttagcgaagg tcgccgatcg tttgcccgta aaactaactg 360 gcaaaagcct aagtgctgtt tcgggtgcaa tggtataact tcccacattt agccgaatag 420 cgccaacagc gttattattt ggaaccaagc agtgggcgat gtcagcgaca attccacttt 480 ggtagaggtc gttcttttgt agaaaacaat ctaacataac tatgacactt tcggtaaatt 540 caccaccttt accagggtcg taagtggtca agccacgccg accacttcat aagcattggt 600 ggaaatcgca tcgacacaat aaaagtacac caaccagcag aaaacaggta catatacagt 660 ttaacgacct ggatctaaga tgactacagt agcatagcca tagaacatgg cactgaaata 720 tcgatgggcg taaaagtgaa caacgcagac gatggattac gacgccaagc cactataaat 780 gctaattgag tcgaagaagg tactcctttt tggaacacag atattgcaga agcgggaatg 840 aggttgatat tacatcaagt atcaacagac gacctcagaa gggtggtctt ttaaaatgac 900 aacgtacatt aagatgcaga caaacactac tatgctggat gtgaaaatgt catatgtgcg 960 gcaaaaatag acgacattta agaacctagc attataggat ggagtgttgt attaattcac 1020 tctagagtaa tggatcacgg tgccagaaac taaggtggtg taggactaga tgcaagtaac 1080 aacggtcgta ctaaaatcag ctacgcgatc gccgacttag aaggctgaga tggagaaact 1140 tgtctactgg cagacctcac tatcttttag gaaggtcacg aagcggctgg agtgatggct 1200 agtctcagcc actgtcaacg ggaagcgcga cgtttatggt caatataaac tagcagttta 1260 atccgtatgg cgatcttagc ggatgttaca ggatggagaa gatgacaaag tagaagctag 1320 acaactatat caaatcgttg tgtggtctta aaccgacaca gtagcattca tcgttggatg 1380 tggaaggtca cagcctcgtt ggaggagata ctacggtccc gcttttcgtg ggtgacaaag 1440 gtcgcaaagg tttccttagg gaggtcgttt cccggatctt tatctacatc tcctgggtga 1500 aggggatgat ctttctctac atagattgca gagagccagg tcaaatacat tgagaaatct 1560 gtatatgtac tttggtaacg ctctaaggta ggtacacgtt agtgatataa aaagaaggcg 1620 acgacgaact taaattgtta gagcagttta tgggtggtag gttacatcta tttacttcaa 1680 aacgcggtgc cctagcccac aagcattgct ttgtaaaatt gttgaaggta acttgtaaaa 1740 gccgaacatg aacgatggat agtagaccgg gaagaccaac gttttttcac tactccgaaa 1800 gaacacactg aagggaacgg tgtaggttcg gtgagtccgg gactaggtgt ccatgtcgga 1860 gacctcagtg gtgacgtcct cgtagtatag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1920 <210> 38 <211> 2014 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 38 gacttgtagg cccatgaatt acccgcttag gcaatgggta gacctttcct tgccgcgtgg 60 aacaaaccgc catgagtgtt ttacgtttac ggtcggcgac cggagtcaca gcgcgaccaa 120 cggggccagg caaaggccca taggcttttc catcggcacg cgaccgattc catcaagtgt 180 taggtgcaat ccccattcgg gacggacacg cgagagcgtt agtcggcgcg tgcgcgggac 240 tatttagcga aggtcgccgt atcgcgggtg gcggcgtttc cgccataaca acaagcgttt 300 aaggtcagag tccgcgacga atgaaacgcc gtgcaagcgg ctttggtacg gccagtttag 360 ccaaagcctg atccgttcgc cttgggttat catgtaccag tcaagcggtc atcgcgggcg 420 gcgttatcat ggatcgtttg cccgtaaaac taactggcaa aagcctaagt gctgtttcgg 480 gtgcaatggt ataacttccc acatttagcc gaatagcgcc aacagcgtta ttatttggaa 540 ccaagcagtg ggcgatgtca gcgacaattc cactttggta gaggtcgttc ttttgtagaa 600 aacaatctaa cataactatg acactttcgg taaattcacc acctttacca gggtcgtaag 660 tggtcaagcc acgccgacca cttcataagc attggtggaa atcgcatcga cacaataaaa 720 gtacaccaac cagcagaaaa caggtacata tacagtttaa cgacctggat ctaagatgac 780 tacagtagca tagccataga acatggcact gaaatatcga tgggcgtaaa agtgaacaac 840 gcagacgatg gattacgacg ccaagccact ataaatgcta attgagtcga agaaggtact 900 cctttttgga acacagatat tgcagaagcg ggaatgaggt tgatattaca tcaagtatca 960 acagacgacc tcagaagggt ggtcttttaa aatgacaacg tacattaaga tgcagacaaa 1020 cactactatg ctggatgtga aaatgtcata tgtgcggcaa aaatagacga catttaagaa 1080 cctagcatta taggatggag tgttgtatta attcactcta gagtaatgga tcacggtgcc 1140 agaaactaag gtggtgtagg actagatgca agtaacaacg gtcgtactaa aatcagctac 1200 gcgatcgccg acttagaagg ctgagatgga gaaacttgtc tactggcaga cctcactatc 1260 ttttaggaag gtcacgaagc ggctggagtg atggctagtc tcagccactg tcaacgggaa 1320 gcgcgacgtt tatggtcaat ataaactagc agtttaatcc gtatggcgat cttagcggat 1380 gttacaggat ggagaagatg acaaagtaga agctagacaa ctatatcaaa tcgttgtgtg 1440 gtcttaaacc gacacagtag cattcatcgt tggatgtgga aggtcacagc ctcgttggag 1500 gagatactac ggtcccgctt ttcgtgggtg acaaaggtcg caaaggtttc cttagggagg 1560 tcgtttcccg gatctttatc tacatctcct gggtgaaggg gatgatcttt ctctacatag 1620 attgcagaga gccaggtcaa atacattgag aaatctgtat atgtactttg gtaacgctct 1680 aaggtaggta cacgttagtg atataaaaag aaggcgacga cgaacttaaa ttgttagagc 1740 agtttatggg tggtaggtta catctattta cttcaaaacg cggtgcccta gcccacaagc 1800 attgctttgt aaaattgttg aaggtaactt gtaaaagccg aacatgaacg atggatagta 1860 gaccgggaag accaacgttt tttcactact ccgaaagaac acactgaagg gaacggtgta 1920 ggttcggtga gtccgggact aggtgtccat gtcggagacc tcagtggtga cgtcctcgta 1980 gtataaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 2014 <210> 39 <211> 556 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 39 gtcacctaaa cgtgcgaagt gtgcgaaatt accaccgtaa attcgtccta ctgaaagtgc 60 ttacgcatct tgagagagat tgcagagagc caggtcaaat acattgagaa atctgtatat 120 gtactttggt aacgctctaa ggtaggtaca cgttagtgat ataaaaagaa ggcgacgacg 180 gtagcagagg tctagaaacc gcatgaacga ccaacttatt tgaagagcta gtgcggtgga 240 attgggtcgg aagaacttaa attgttagag cagtttatgg gtggtaggtt acatctattt 300 acttcaaaac gcggtgccct agcccacaag cattgctttg taaaattgtt gaaggtaact 360 tgtaaaagcc gaacatgaac gatggatagt agaccgggaa gaccaacgtt ttttcactac 420 tccgaaagaa cacactgaag ggaacggtgt aggttcggtg agtccgggac taggtgtcca 480 tgtcggagac ctcagtggtg acgtcctcgt agtataggtc cacgctaaaa aaaaaaaaaa 540 aaaaaaaaaa aaaaaa 556 <210> 40 <211> 2503 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 40 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 60 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 120 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 180 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 240 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 300 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 360 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 420 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 480 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 540 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 600 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 660 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 720 taaagcaagg tgcgcaatag taaccttggt aacttcggaa ctgcgtcaag ccgctaactc 780 catcggtatg gaagtcgcta aagtcggtca aacggaagcg gctatcgtcg caactagtcg 840 aatagttgtc gaagaggcca gagcaaccat ggttgccatt gtcttcgtgg tctatgcaag 900 acgtccttgc gtcatggcct aagcatcttc ttcactggtt gctatagcgg aagtcgttac 960 cgaactggtc gccagtcgaa tcgtttgccc catggtagtc atcaccacca tcgcggaaac 1020 caacggtccg aactcacggt aggtagtcca cgtatgtagc ccacctttcg aaggaatcgt 1080 ggtcgccggt ctcctagaaa cggtatatgt cttgcgtcac gcttatggac cggtcatcct 1140 ggccagtagc cgacttgtgg tcgctatagc tttcgctagt ccctgcctta acgtgcagca 1200 tggtatggct attgtcgtcc cggctacaac cactatcgtg gtcttgaaac tggtcgtcgc 1260 tacaaccatc ctagcattgg caccagcggt cgttgaagcc gaaggtagtc tctaactggt 1320 tttcgaagct agtcaaacag tagcgccata tggcctagcc tgaaccttag aaaaaggagt 1380 cgaaggtcct aaacccgctg gtcgcgaacg aacaaaaacg gtcgttaaag gccgtccgga 1440 agcggaaggt cgtcgcccca ggtgaacaag tcctaaagaa acaaaaagtc tctgtagtct 1500 atttaaaacc caacgcaaac gcgaacgcag aatcgtctga acccacgtcc atcattacgg 1560 attatataag gtttctgttt cgttaactag gtagttattg atgtcatccg cactacaagc 1620 taacaggagg taacagatag agcatgagtg gtagtaggga tgaaacagga agtactaatt 1680 acatacaggg actcctggtt gggtgacccc gggacacgta cgtgtcctaa taaacaggag 1740 gcgggtggtt tcctatctac tacggacagg gattttcccc gagtagcgaa ggatatgaaa 1800 cttctagtta tagacatata agacgtagtc caggaagtcg gctgagtcca tgaggtcagt 1860 tttggaggag aagtagttgt cacgatcctt agttaagata acaaagtccg agtagttatc 1920 acgacttgct gactgcgtcc actatgtttc cggggtgggc atggggactt gcacacggat 1980 aactccatcc ggattattaa gacgaggtgg ggtgtgctgg gctcccagga acttcatcgg 2040 agtgacgtag aaccttcagt ttgagtccac gtaggacacc agcgctattg gtgccgacga 2100 cgagtacaaa catccggaaa cttagcatat taggtaagag tttctgtatg ctcggacgtc 2160 gtcgttagag cagacccaga aggtgtccat ggacacacat catacaagac aacttggtca 2220 acttttacgg ggctggggcc aattatcccg ggagtcctca cgatcagaaa ccgactggtt 2280 tgtcacactc tggttagagg aggggtcacc acgatgggtg agggggtggt gggtgtttat 2340 acaagagtct gggactccaa agacgatatt acgacgagac gacttatcca cgtactttgt 2400 ttcccaaacc cacgcaactc cacctttagt ccacacaaac tagtacgaga cacccggatt 2460 caaggtcgtg accaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 2503 <210> 41 <211> 2059 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 41 ggaccgaagc atactgcaag acttgtaggc ccatgaatta cccgcttagg caatgggtag 60 acctttcctt gccgcgtgga acaaaccgcc atgagtgttt tacgtttacg gtcggcgacc 120 ggagtcacag cgcgaccaac ggggccaggc aaaggcccat aggcttttcc atcggcacgc 180 gaccgattcc atcaagtgtt aggtgcaatc cccattcggg acggacacgc gagagcgtta 240 gtcggcgcgt gcgcgggact atttagcgaa ggtcgccgat cgtttgcccg taaaactaac 300 tggcaaaagc ctaagtgctg tttcgggtgc aatggtataa cttcccacat ttagccgaat 360 agcgccaaca gcgttattat ttggaaccaa gcagtgggcg atgtcagcga caattccact 420 ttggtagagg tcgttctttt gtagaaaaca atctaacata actatgacac tttcggtaaa 480 ttcaccacct ttaccagggt cgtaagtggt caagccacgc cgaccacttc ataagcattg 540 gtggaaatcg catcgacaca ataaaagtac accaaccagc agaaaacagg tacatataca 600 gtttaacgac ctggatctaa gatgactaca gtagcatagc catagaacat ggcactgaaa 660 tatcgatggg cgtaaaagtg aacaacgcag acgatggatt acgacgccaa gccactataa 720 atgctaattg agtcgaagaa ggtactcctt tttggaacac agatattgca gaagcgggaa 780 tgaggttgat attacatcaa gtatcaacag acgacctcag aagggtggtc ttttaaaatg 840 acaacgtaca ttaagatgca gacaaacact actatgctgg agtgttgtat taattcactc 900 tagagtaatg gatcacggtg ccagaaacta aggtggtgta ggactagatg caagtaacaa 960 cggtcgtact aaaatcagct acgcgatcgc cgacttagaa ggctgagatg gagaaacttg 1020 tctactggca gacctcacta tcttttagga aggtcacgaa gcggctggag tgatggctag 1080 tctcagccac tgtcaacggg aagcgcgacg tttatggtca atataaacta gcagtttaat 1140 ccgtatggcg atcttagcgg atgttacagg atggagaaga tgacaaagta gaagctagac 1200 aactatatca aatcgttgtg tggtcttaaa ccgacacagt agcattcatc gttggatgtg 1260 gaaggtcaca gcctcgttgg aggagatact acggtcccgc ttttcgtggg tgacaaaggt 1320 cgcaaaggtt tccttaggga ggtcgtttcc cggatcttta tctacatctc ctgggtgaag 1380 gggatgatct ttctctacat gtcctgggtg gaggggagtt tccttgtatg ggtggaaccc 1440 aacccgccgg tgagggtgag ttacggtcgg cggtcatatg tgggtatgac gggtatgacg 1500 tcataaatag tcgttgtcaa aatgggaggt ggaagtcctt actgtagtca cccgtgaagg 1560 tggagaactg gcaggacatc tctaggtcac ctaaacgtgc gaagtgtgcg aaattaccac 1620 cgtaaattcg tcctactgaa agtgcttacg catcttgaga gagattgcag agagccaggt 1680 caaatacatt gagaaatctg tatatgtact ttggtaacgc tctaaggtag gtacacgtta 1740 gtgatataaa aagaaggcga cgacgaactt aaattgttag agcagtttat gggtggtagg 1800 ttacatctat ttacttcaaa acgcggtgcc ctagcccaca agcattgctt tgtaaaattg 1860 ttgaaggtaa cttgtaaaag ccgaacatga acgatggata gtagaccggg aagaccaacg 1920 ttttttcact actccgaaag aacacactga agggaacggt gtaggttcgg tgagtccggg 1980 actaggtgtc catgtcggag acctcagtgg tgacgtcctc gtagtataga aaaaaaaaaa 2040 aaaaaaaaaa aaaaaaaaa 2059 <210> 42 <211> 582 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 42 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggggccag gcaaaggccc ataggctttt ccatcggcac gcgaccgatt 180 ccatcaagta tagccacggt cgtaacttcg acggcggcaa tagtttatgg cgctaacacc 240 tttccatcgc tgagtccaaa cgtatgacgt gaggtgttag gtgcaatccc cattcgggac 300 ggacacgcga gtaaaggtct aacatgtttc aactttccag ttagtcgccg tcctatattg 360 gcaagaccgg atggccgtca accagttcca ttctgactat ttgccacatt gattaaagaa 420 gtaggagcgt tagtcggcgc gtgcgcggga ctatttagcg aaggtcgccg tatcgcgggt 480 ggcggcgttt ccgccataac aacaagcgtt taaggtcaga gtccgcgacg aatgaaacgc 540 cgtgcaagcg gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 582 <210> 43 <211> 563 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 43 ggcccatgaa ttacccgctt aggcaatggg tagacctttc cttgccgcgt ggaacaaacc 60 gccatgagtg ttttacgttt acggtcggcg accggagtca cagcgcgacc aacggggcca 120 gggaggaagc cttttagcca tcgctatgcc gccgctcaaa cgcgccgggt gcaaaagccc 180 agagcaaagg cccataggct tttccatcgg cacgcgaccg attccatcaa gtgttaggtg 240 caatccccat tcgggacgga cacgcgagag cgttagtcgg cgcgtgcgcg ggactattta 300 gcgaaggtcg ccgatcgttt gcccgtaaaa ctaactggca aaagcctaag tgctgtttcg 360 ggtgcaatgg tataacttcc cacatttagc cgaatagcgc caacagcgtt attatttgga 420 accaagcagt gggcgatgtc agcgacaatt ccactttggt agaggtcgtt cttttgtaga 480 aaacaatcta acataactat gacactttcg gtaaattcac cacctttacc aggaaaaaaa 540 aaaaaaaaaa aaaaaaaaaa aaa 563 <210> 44 <211> 2115 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 44 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggggccag gcaaaggccc ataggctttt ccatcggcac gcgaccgatt 180 ccatcaagtg ttaggtgcaa tccccattcg ggacggacac gcgagagcgt tagtcggcgc 240 gtgcgcggga ctatttagcg aaggtcgccg atcgtttgcc cgtaaaacta actggcaaaa 300 gcctaagtgc tgtttcgggt gcaatggtat aacttcccac atttagccga atagcgccaa 360 cagcgttatt atttggaacc aagcagtggg cgatgtcagc gacaattcca ctttggtaga 420 ggtcgttctt ttgtagaaaa caatctaaca taactatgac actttcggta aattcaccac 480 ctttaccagg gtcgtaagtg gtcaagccac gccgaccact tcataagcat tggtggaaat 540 cgcatcgaca caataaaagt acaccaacca gcagaaaaca ggtacatata cagtttaacg 600 acctggatct aagatgacta cagtagcata gccatagaac atggcactga aatatcgatg 660 ggcgtaaaag tgaacaacgc agacgatgga ttacgacgcc aagccactat aaatgctaat 720 tgagtcgaag aaggtactcc tttttggaac acagatattg cagaagcggg aatgaggttg 780 atattacatc aagtatcaac agacgacctc agaagggtgg tcttttaaaa tgacaacgta 840 cattaagatg cagacaaaca ctactatgct ggatgtgaaa atgtcatatg tgcggcaaaa 900 atagacgaca tttaagaacc tagcattata ggatggagtg ttgtattaat tcactctaga 960 gtaatggatc acggtgccag aaactaaggt ggtgtaggac tagatgcaag taacaacggt 1020 cgtactaaaa tcagctacgc gatcgccgac ttagaaggct gagatggaga aacttgtcta 1080 ctggcagacc tcactatctt ttaggaaggt cacgaagcgg ctggagtgat ggctagtctc 1140 agccactgtc aacgggaagc gcgacgttta tggtcaatat aaactagcag tttaatccgt 1200 atggcgatct tagcggatgt tacaggatgg agaagatgac aaagtagaag ctagacaact 1260 atatcaaatc gttgtgtggt cttaaaccga cacagtagca ttcatcgttg gatgtggaag 1320 gtcacagcct cgttggagga gatactacgg tcccgctttt cgtgggtgac aaaggtcgca 1380 aaggtttcct tagggaggtc gtttcccgga tctttatcta catctcctgg gtgaagggga 1440 tgatctttct ctacatgtcc tgggtggagg ggagtttcct tgtatgggtg gaacccaacc 1500 cgccggtgag ggtgagttac ggtcggcggt catatgtggg tatgacgggt atgacgtcat 1560 aaatagtcgt tgtcaaaatg ggaggtggaa gtccttactg tagtcacccg tgaaggtgga 1620 gaactggcag gacatctcta ggtcacctaa acgtgcgaag tgtgcgaaat taccaccgta 1680 aattcgtcct actgaaagtg cttacgcatc ttgagagaga ttgcagagag ccaggtcaaa 1740 tacattgaga aatctgtata tgtactttgg taacgctcta aggtaggtac acgttagtga 1800 tataaaaaga aggcgacgac gaacttaaat tgttagagca gtttatgggt ggtaggttac 1860 atctatttac ttcaaaacgc ggtgccctag cccacaagca ttgctttgta aaattgttga 1920 aggtaacttg taaaagccga acatgaacga tggatagtag accgggaaga ccaacgtttt 1980 ttcactactc cgaaagaaca cactgaaggg aacggtgtag gttcggtgag tccgggacta 2040 ggtgtccatg tcggagacct cagtggtgac gtcctcgtag tatagaaaaa aaaaaaaaaa 2100 aaaaaaaaaa aaaaa 2115 <210> 45 <211> 915 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 45 gtatatgtac tttggtaacg ctctaaggta ggtacacgtt agtgatataa aaagaaggcg 60 acgacgaact taaattgtta gagcagttta tgggtggtag gttacatcta tttacttcaa 120 aacgcggtgc cctagcccac aagcattgct ttgtaaaatt gttgaaggta acttgtaaaa 180 gccgaacatg aacgatggat agtagaccgg gaagaccaac gttttttcac tactccgaaa 240 gaacacactg aagggaacgg tgtaggttcg gtgagtccgg gactaggtgt ccatgtcgga 300 gacctcagtg gtgacgtcct cgtagtatag ctgctcgcat gaccagctat atctggaaca 360 tcgctggtgg gtcttatggt cgcttttgtg ggaaccatca tagtcgcaag tctggccttc 420 ggtatggaac tcgtcgtccc gactacaacc atagctagtc tcggtctcgg ctttgttgca 480 atgcccttcg ttactacttc aacggtcgtg gtgccggtca aaccgctcgc taaagcaagc 540 gaagaaacag ctaaacttag ttgtgtcgaa acatcctaag tggtcgggtt gctcgtggag 600 catgcagcta gcccagaacc tttagcttgt gtggtcttcg tctcggtagt cagaaccagg 660 tcggtctgaa tggttggact cgccacatca actaggtcag ctattgaagt gccaactaaa 720 ggtcgtattg aagcaatcgg tcctatggca agtcaagtga cagtcgtagg tatggcactt 780 gccgcgcctc cagaactcgg tcaagctacc tcataactcg gaagtcgact gctctaaagc 840 gaagttgtca aacgaacagc tatgccttcg agtaaagcaa ggtgcaaaaa aaaaaaaaaa 900 aaaaaaaaaa aaaaa 915 <210> 46 <211> 2504 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 46 gcccatgaat tacccgctta ggcaatgggt agacctttcc ttgccgcgtg gaacaaaccg 60 ccatgagtgt tttacgttta cggtcggcga ccggagtcac agcgcgacca acggggccag 120 gcaaaggccc ataggctttt ccatcggcac gcgaccgatt ccatcaagtg ttaggtgcaa 180 tccccattcg ggacggacac gcgagagcgt tagtcggcgc gtgcgcggga ctatttagcg 240 aaggtcgccg atcgtttgcc cgtaaaacta actggcaaaa gcctaagtgc tgtttcgggt 300 gcaatggtat aacttcccac atttagccga atagcgccaa cagcgttatt atttggaacc 360 aagcagtggg cgatgtcagc gacaattcca ctttggtaga ggtcgttctt ttgtagaaaa 420 caatctaaca taactatgac actttcggta aattcaccac ctttaccagg gtcgtaagtg 480 gtcaagccac gccgaccact tcataagcat tggtggaaat cgcatcgaca caataaaagt 540 acaccaacca gcagaaaaca ggtacatata cagtttaacg acctggatct aagatgacta 600 cagtagcata gccatagaac atggcactga aatatcgatg ggcgtaaaag tgaacaacgc 660 agacgatgga ttacgacgcc aagccactat aaatgctaat tgagtcgaag aaggtactcc 720 tttttggaac acagatattg cagaagcggg aatgaggttg atattacatc aagtatcaac 780 agacgacctc agaagggtgg tcttttaaaa tgacaacgta cattaagatg cagacaaaca 840 ctactatgct ggagtgttgt attaattcac tctagagtaa tggatcacgg tgccagaaac 900 taaggtggtg taggactaga tgcaagtaac aacggtcgta ctaaaatcag ctacgcgatc 960 gccgacttag aaggctgaga tggagaaact tgtctactgg cagacctcac tatcttttag 1020 gaaggtcacg aagcggctgg agtgatggct agtctcagcc actgtcaacg ggaagcgcga 1080 cgtttatggt caatataaac tagcagttta atccgtatgg cgatcttagc ggatgttaca 1140 ggatggagaa gatgacaaag tagaagctag acaactatat caaatcgttg tgtggtctta 1200 aaccgacaca gtagcattca tcgttggatg tggaaggtca cagcctcgtt ggaggagata 1260 ctacggtccc gcttttcgtg ggtgacaaag gtcgcaaagg tttccttagg gaggtcgttt 1320 cccggatctt tatctacatc tcctgggtga aggggatgat ctttctctac atagattgca 1380 gagagccagg tcaaatacat tgagaaatct gtatatgtac tttggtaacg ctctaaggta 1440 ggtacacgtt agtgatataa aaagaaggcg acgacgaact taaattgtta gagcagttta 1500 tgggtggtag gttacatcta tttacttcaa aacgcggtgc cctagcccac aagcattgct 1560 ttgtaaaatt gttgaaggta acttgtaaaa gccgaacatg aacgatggat agtagaccgg 1620 gaagaccaac gttttttcac tactccgaaa gaacacactg aagggaacgg tgtaggttcg 1680 gtgagtccgg gactaggtgt ccatgtcgga gacctcagtg gtgacgtcct cgtagtatag 1740 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 1800 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 1860 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 1920 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 1980 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 2040 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 2100 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 2160 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 2220 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 2280 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 2340 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 2400 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 2460 taaagcaagg tgcgaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 2504 <210> 47 <211> 576 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 47 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acgggcaaag gcccataggc ttttccatcg gcacgcgacc gattccatca 180 agtatagcca cggtcgtaac ttcgacggcg gcaatagttt atggcgctaa cacctttcca 240 tcgctgagtc caaacgtatg acgtgaggtg ttaggtgcaa tccccattcg ggacggacac 300 gcgagtaaag gtctaacatg tttcaacttt ccagttagtc gccgtcctat attggcaaga 360 ccggatggcc gtcaaccagt tccattctga ctatttgcca cattgattaa agaagtagga 420 gcgttagtcg gcgcgtgcgc gggactattt agcgaaggtc gccgtatcgc gggtggcggc 480 gtttccgcca taacaacaag cgtttaaggt cagagtccgc gacgaatgaa acgccgtgca 540 agcggcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 576 <210> 48 <211> 259 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 48 gtaccaaagc cgcttgcacg gcgtttcatt cgtcgcggac tctgacctta aacgcttgtt 60 gttatggcgg aaacgccgcc acccgcgata cggcgacctt cgctaaatag tcccgcgcac 120 gcgccgacta acgctcctac ttctttaatc aatgtggcaa atagtcagaa tggaactggt 180 tgacggccat ccggtcttgc caatatagga cggcgactaa ctggaaagta aaaaaaaaaa 240 aaaaaaaaaa aaaaaaaaa 259 <210> 49 <211> 1465 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 49 ggcaagacga atctgcgtca acgctaaagg ccgtcaaaaa gcaagagggc gtcgcgcctg 60 ggaaaaaggt agtcaagaaa ccatcaagct taaatgtcgg gaaattgggt ttgcgccgcc 120 ttcaggggca gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact 180 cctcgggtca accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag 240 caaggtgccc aaagtagact gagcgacagt cgaaaccagc cccaatgaac aagacgccat 300 tgcaaaacgt ctatacgcta cggtcaaaga cgcttccaca ccacatgttg tgctcgccat 360 ttcaactagt gcagttgcta aagtgccaac taaggttcgt cttgaagcaa tcggtagtat 420 ctgcagtcaa gtcgtagtcg taggtatggc acttgtcgtg cgtctaggac gcgttgaagc 480 tatcttattg cccgaaggag ccgaaagtct gggacacgtc gtgggacgta tcgttaggac 540 tatgggacta gtcgtcttcg gtcttacaaa tgttgcaatg ccctgtgagc tacttatgaa 600 acatgaatgg tcggtcttgt ggtcgctttt gtcgaaatca ccgaagtgcg tataattgat 660 gaacgagtcg aagtcgaagt tgttcaacaa gagagttagg tagtgccgct tgaaaaagta 720 atagtgcaaa taagaaaaag aagcgaaacg gcattcgttt aacaaaacgt cacatattta 780 ggtatagtag attgcgttca caaagaaatt taaatttaat ttaccatttc gtcattgtat 840 atgcggtaaa cgtaaattta cacatatgac acgaactttg agttttatta gcctgcctaa 900 ttgttggtaa caatgtaatg gaaattggcg atgactatgg ggaaagagca atcaactcac 960 cataaatttg tgaacgcagt taagtaattg gcatcgcgcg cctaatcgtt tacattcgca 1020 tggaacaaga agaaagagac ctattcgtgg gagtcaatgg ctatgtggaa aaacatgaac 1080 tgccaaattc ggctaaccaa ccagaaatgg tcatggaaaa ttcttgcgtc aaagttcgcg 1140 gtgacgttta cgaagaagct aaacttgtgc ttgtttagaa agttaggtgc gtcctggttt 1200 tagtaaattt agaaaaccat tttgttgttt gcgacagtta aataaaactt aacgacaaag 1260 aagtgacttg cgtcgtagat taactggaaa ttaaagtggt atctaaaatg gggtttgccg 1320 atgcggtctt caaagtctaa aattcttgaa gtttgcgttt ggtcgcaaaa attatggatt 1380 tagtcgatta ttgcctacta agcagcagtt tagaagtttt taagatagtt acgctaaaaa 1440 aaaaaaaaaa aaaaaaaaaa aaaaa 1465 <210> 50 <211> 604 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 50 ggggcagcga tgggggcgac aattgtggac cgtatggact ccattatgct gggactcctc 60 gggtcaaccg ctctaaagcg aagttgttgg acaaacagtt atgcgtaact gtaaagcaag 120 gtgcccaaag tagactgagc gacagtcgaa accagcccca atgaacaaga cgccattgca 180 aaacgtctat acgctacggt caaagacgct tccacaccac atggactatg ggactagtcg 240 tcttcggtct tacaaatgtt gcaatgccct gtgagctact tatgaaacat gaatggtcgg 300 tcttgtggtc gcttttgtcg aaatcaccga agtgcgtata attgatgaac gagtcgaagt 360 cgaagttgtt caacaagaga gttaggtagt gccgcttgaa aaagtaatag tgcaaataag 420 aaaaagaagc gaaacggcat tcgtttaaca aaacgtcaca tatttaggta tagtagattg 480 cgttcacaaa gaaatttaaa tttaatttac catttcgtca ttgtatatgc ggtaaacgta 540 aatttacaca tatgacacga actttgagtt ttataaaaaa aaaaaaaaaa aaaaaaaaaa 600 aaaa 604 <210> 51 <211> 1999 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 51 gtgggtttat atcgcgtatc catccaactt aactgaaacg cgaagcagta gacagtgttg 60 atcgagattt gcgaagggaa acagtaaaag tccagggtgt cgttaattac gacaaaacta 120 aagactgtat agttagaagg atcaattggg ctgatttaga aatggtataa gttaaagaaa 180 tggaaactta ttcgacaaaa agaaaaactc atgatgtata gatggaaact gccataatgt 240 cgattcgagt tatattttta gattttggta aaatatccaa catgtattaa cgaaattttg 300 aagtatagat caaactattc ttcaggtaca gcatcaagca ttcggtagca taagccaaac 360 aagtctttga agggttggac atagcgagtt gtgtagaaag ttacgaagac ttcaacagaa 420 aaagagttta aaaggaccac gtatgccaaa tcgacaacaa tatagtcgtt ctattctgcg 480 atatgtaaga tagagacaca tcggcaggta gcatagaact tgggtcaatt attatttaag 540 tgcggtaaca aacaaactta gggaatatta ttgtgctaca gcaaatgcct tgaaaagtcg 600 agaaagacta ctatactcgt caagttcgta aagacttaac agtaattgta atgcaaatac 660 ttagcgagat tgattccttc ccagtagtca tctttccttt agatattaga acagcaattt 720 tagattttgt acatagtgga gaaaaggacg tcattttaat ggatgaacca gacgttaacg 780 tcatttactt ggtcgttgat ttcgaagact cagtcatcaa aattgtagct tcgctagcta 840 atgtgcttaa aatcatcata gtcaaagtgt acgtggtcgt ggtaataaac aaccttcatt 900 actgtacaaa agtgccagtt tgttacatta tcgaacgctg tatagaggta tcatttcacc 960 tatttgacaa ctaggtgata aaccttcggt cgctagacgt tgttgttatt gaaaaaatgg 1020 aagataatct atacttaatc caaaacaacc cagaccaagc tcgtatataa atggaagtcg 1080 tcaaaattgg tttcgttcaa ataagttggg actgatgaat cagttttctg aacttcaccg 1140 ctattagcgt cctagttgtc tacataatta gtatcattat tgtagtagtt taaatcgcta 1200 acaaccaaaa aatccttgac atgggtaacg acgttattgg ggaaccaatg ctcagtgttg 1260 gtgaaagaat cgttaggtcg aactattggt acgttctaat aaaagacgac atagaaattc 1320 gttacataat caacgtcact actaagggcg tattgcaaat tagttcataa attattaaca 1380 gtttgcacct aatagttcta gtccttaaaa aggtagaaga cgtaggtaat gtcagttgcc 1440 tcaatgtagt caggtaaaaa gtatatgtgc tgatggcaaa cttcatcttt gtgagagttg 1500 agcatgtata acaacatcac tcagtataac cgcttggtta cacaattagt atcatgccta 1560 tagaacgcct tatcattcag gtcgtcgtga tagtgaaggg gatcaaagtc aaaatctact 1620 ttgtcataac tctttacggt atcccaatgc ttggtataag tgcttaagaa attgtttaaa 1680 cagaaactca agtataagcg aaaacaagtt acctggaaaa agaagaagtg cgtataattg 1740 atgaacgagt cgaagtcgaa gttgttcaac aagagagtta ggtagtgccg cttgaaaaag 1800 taatagtgca aataagaaag taagactgta tcggagacga ctttttaata gtagtaattc 1860 tcggtacaac taaaccagta atttcagaca ctgaaataga ggacttcgtt aggtacgaag 1920 caagttaact aaatgacaat taaattgagg gtaaatatca caaagaaata aaaaaaaaaa 1980 aaaaaaaaaa aaaaaaaaa 1999 <210> 52 <211> 1567 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 52 gcttaaatgt cgggaaattg ggtttgcgcc gccttcaggg gcagcgatgg gggcgacaat 60 tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc taaagcgaag 120 ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag actgagcgac 180 agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg ctacggtcaa 240 agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc gcacaagtcg 300 gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc ctcacgagta 360 gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg cttggtatgg 420 ctattgtcgg cctggatgca attgtgctcg ccatttcaac tagtgcagtt gctaaagtgc 480 caactaaggt tcgtcttgaa gcaatcggta gtatctgcag tcaagtcgta gtcgtaggta 540 tggcacttgt cgtgcgtcta ggacgcgttg aagctatctt attgcccgaa ggagccgaaa 600 gtctgggaca cgtcgtggga cgtatcgtta ggactatggg actagtcgtc ttcggtctta 660 caaatgttgc aatgccctgt gagctactta tgaaacatga atggtcggtc ttgtggtcgc 720 ttttgtcgaa atcaccgaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca 780 acaagagagt taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagcgaaac 840 ggcattcgtt taacaaaacg tcacatattt aggtatagta gattgcgttc acaaagaaat 900 ttaaatttaa tttaccattt cgtcattgta tatgcggtaa acgtaaattt acacatatga 960 cacgaacttt gagttttatt agcctgccta attgttggta acaatgtaat ggaaattggc 1020 gatgactatg gggaaagagc aatcaactca ccataaattt gtgaacgcag ttaagtaatt 1080 ggcatcgcgc gcctaatcgt ttacattcgc atggaacaag aagaaagaga cctattcgtg 1140 ggagtcaatg gctatgtgga aaaacatgaa ctgccaaatt cggctaacca accagaaatg 1200 gtcatggaaa attcttgcgt caaagttcgc ggtgacgttt acgaagaagc taaacttgtg 1260 cttgtttaga aagttaggtg cgtcctggtt ttagtaaatt tagaaaacca ttttgttgtt 1320 tgcgacagtt aaataaaact taacgacaaa gaagtgactt gcgtcgtaga ttaactggaa 1380 attaaagtgg tatctaaaat ggggtttgcc gatgcggtct tcaaagtcta aaattcttga 1440 agtttgcgtt tggtcgcaaa aattatggat ttagtcgatt attgcctact aagcagcagt 1500 ttagaagttt ttaagatagt tacgctagaa gaccgggaaa aaaaaaaaaa aaaaaaaaaa 1560 aaaaaaa 1567 <210> 53 <211> 1118 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 53 gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgggtca 60 accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag caaggtgccc 120 aaagtagact gagcgacagt cgaaaccagc cccaatgaac aagacgccat tgcaaaacgt 180 ctatacgcta cggtcaaaga cgcttccaca ccacatgctc tcatgtagcc taccttacga 240 agaaatcgca caagtcggtc gcccagcggt ggcatatgtc ttgcctaacg tttctagacc 300 gatcagcctc acgagtagcc tgcttgtggt gtttatagtt tacgctagtc caatctgtat 360 cgtgccgctt ggtatggcta ttgtcggcct ggatgcaatt gtgctcgcca tttcaactag 420 tgcagttgct aaagtgccaa ctaaggttcg tcttgaagca atcggtagta tctgcagtca 480 agtcgtagtc gtaggtatgg cacttgtcgt gcgtctagga cgcgttgaag ctatcttatt 540 gcccgaagga gccgaaagtc tgggacacgt cgtgggacgt atcgttagga ctatgggact 600 agtcgtcttc ggtcttacaa atgttgcaat gccctgtgag ctacttatga aacatgaatg 660 gtcggtcttg tggtcgcttt tgtcgaaatc accgaagtgc gtataattga tgaacgagtc 720 gaagtcgaag ttgttcaaca agagagttag gtagtgccgc ttgaaaaagt aatagtgcaa 780 ataagaaaaa gaagcgaaac ggcattcgtt taacaaaacg tcacatattt aggtatagta 840 gattgcgttc acaaagaaat ttaaagtctt gtaattatgg tggtcgattt attggtagta 900 tatttggaaa tagcatgcaa ttttggaatg gacgaagaac ctgacatctg agctattgta 960 agtttaattt accatttcgt cattgtatat gcggtaaacg taaatttaca catatgacac 1020 gaactttgag ttttattagc ctgcctaatt gttggtaaca atgtaatgga aattggcgat 1080 gactatggaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1118 <210> 54 <211> 575 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 54 gaatattgtt tcaatggtcg tggcgctggt cagactgctc gctaaagtcg tcgaagaaac 60 agctagacca tctattctct gaacttccta agtggtcggg ttggtcgtgg cgctcatagt 120 tagcccagaa cgtccagttt atgtggtggt gatcttggtt gccagactca gtatggtcaa 180 aatagttgtg ctcgccattt caactagtgc agttgctaaa gtgccaacta aggttcgtct 240 tgaagcaatc ggtagtatct gcagtcaagt cgtagtcgta ggtatggcac ttgtcgtgcg 300 tctaggacgc gttgaagcta tcttattgcc cgaaggagcc gaaagtctgg gacacgtcgt 360 gggacgtatc gttaggacta tgggactagt cgtcttcggt cttacaaatg ttgcaatgcc 420 ctgtgagcta cttatgaaac atgaatggtc ggtcttgtgg tcgcttttgt cgaaatcacc 480 gaagtgcgta taattgatga acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta 540 gtgccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 575 <210> 55 <211> 604 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 55 gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgggtca 60 accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag caaggtgccc 120 aagtagactc ttactaattt gggaacgacg gaaagtcgct accttcatcg gtatggcttt 180 cgctaaagtc ggtcaaatgg aagaaggtag tcgaagtact gttgaaagag tagactgagc 240 gacagtcgaa accagcccca atgaacaaga cgccattgca aaacgtctat acgctacggt 300 caaagacgct tccacaccac atgctctcat gtagcctacc ttacgaagaa atcgcacaag 360 tcggtcgccc agcggtggca tatgtcttgc ctaacgtttc tagaccgatc agcctcacga 420 gtagcctgct tgtggtgttt atagtttacg ctagtccaat ctgtatcgtg ccgcttggta 480 tggctattgt cggcctggat gcaattgtgc tcgccatttc aactagtgca gttgctaaag 540 tgccaactaa ggttcgtctt gaagcaatcg gtagaaaaaa aaaaaaaaaa aaaaaaaaaa 600 aaaa 604 <210> 56 <211> 407 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 56 gcctatgcct cgtatcatct atggtcgtag gtaaagcaat agcggaaaca ttggatgtga 60 cttcatcagg actgctcgta tagtgagcta cttatgaaac atgaatggtc ggtcttgtgg 120 tcgcttttgt cgaaatcacc gaagtgcgta taattgatga acgagtcgaa gtcgaagttg 180 ttcaacaaga gagttaggta gtgccgcttg aaaaagtaat agtgcaaata agaaaaagaa 240 gcgaaacggc attcgtttaa caaaacgtca catatttagg tatagtagat tgcgttcaca 300 aagaaattta aatttaattt accatttcgt cattgtatat gcggtaaacg taaatttaca 360 catatgacac gaactttaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 407 <210> 57 <211> 515 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 57 gggcgacaat tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc 60 taaagcgaag ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag 120 actgagcgac agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg 180 ctacggtcaa agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc 240 gcacaagtcg gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc 300 ctcacgagta gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg 360 cttggtatgg ctattgtcgg cctggatgca agcactaacg tggtcatcag actggtctgc 420 gctataatct acgcaataga ggcaccagcg gtcgttgaag ccgaagacgc tatttcggtg 480 gcttgaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 515 <210> 58 <211> 1193 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 58 gtgctcgcca tttcaactag tgcagttgct aaagtgccaa ctaaggttcg tcttgaagca 60 atcggtagta tctgcagtca agtcgtagtc gtaggtatgg cacttgtcgt gcgtctagga 120 cgcgttgaag ctatcttatt gcccgaagga gccgaaagtc tgggacacgt cgtgggacgt 180 atcgttagga ctatgggact agtcgtcttc ggtcttacaa atgttgcaat gccctgtgag 240 ctacttatga aacatgaatg gtcggtcttg tggtcgcttt tgtcgaaatc accgaagtgc 300 gtataattga tgaacgagtc gaagtcgaag ttgttcaaca agagagttag gtagtgccgc 360 ttgaaaaagt aatagtgcaa ataagaaagt aagactgtat cggagacgac tttttaatag 420 tagtaattct cggtacaact aaaccagtaa tttcagacac tgaaatagag gacttcgtta 480 ggtacgaagc aagttaacta aatgacaatt aaattgaggg taaatatcac aaagaaatca 540 tggaagattt cgaccgaata caggaaaatt tagattaaag gtttgtcgtc gttgaaataa 600 gaagaagcga aacggcattc gtttaacaaa acgtcacata tttaggtata gtagattgcg 660 ttcacaaaga aatttaaatt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa 720 tttacacata tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt 780 aatggaaatt ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg 840 cagttaagta attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag 900 agacctattc gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa 960 ccaaccagaa atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga 1020 agctaaactt gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa 1080 ccattttgtt gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt 1140 agattaactg gaaattaaag tggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 1193 <210> 59 <211> 484 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 59 ggtagttgtt gaaaaagtag gaatatctag cctagatgtc gtttcaggcg aaggactcta 60 aggtcttaaa atcgttgggt caaccgctct aaagcgaagt tgttggacaa acagttatgc 120 gtaactgtaa agcaaggtgc ccaaagtaga ctgagcgaca gtcgaaacca gccccaatga 180 acaagacgcc attgcaaaac gtctatacgc tacggtcaaa gacgcttcca caccacatgg 240 tatagtggac gccgttagcc tcatgtgtct cactcaaaga ccttgcgtca cggtcatcct 300 ctttgtcagc aacgtagaaa tctccggtct gtcgatacct tgcgtaaagc tctcatgtag 360 cctaccttac gaagaaatcg cacaagtcgg tcgcccagcg gtggcatatg tcttgcctaa 420 cgtttctaga ccgatcagcc tcacgagtag cctgaaaaaa aaaaaaaaaa aaaaaaaaaa 480 aaaa 484 <210> 60 <211> 1558 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 60 gcttaaatgt cgggaaattg ggtttgcgcc gccttcaggg gcagcgatgg gggcgacaat 60 tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc taaagcgaag 120 ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag actgagcgac 180 agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg ctacggtcaa 240 agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc gcacaagtcg 300 gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc ctcacgagta 360 gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg cttggtatgg 420 ctattgtcgg cctggatgca attgtgctcg ccatttcaac tagtgcagtt gctaaagtgc 480 caactaaggt tcgtcttgaa gcaatcggta gtatctgcag tcaagtcgta gtcgtaggta 540 tggcacttgt cgtgcgtcta ggacgcgttg aagctatctt attgcccgaa ggagccgaaa 600 gtctgggaca cgtcgtggga cgtatcgtta ggactatggg actagtcgtc ttcggtctta 660 caaatgttgc aatgccctgt gagctactta tgaaacatga atggtcggtc ttgtggtcgc 720 ttttgtcgaa atcaccgaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca 780 acaagagagt taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagaagcga 840 aacggcattc gtttaacaaa acgtcacata tttaggtata gtagattgcg ttcacaaaga 900 aatttaaatt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 960 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 1020 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 1080 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 1140 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 1200 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 1260 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 1320 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 1380 gaaattaaag tggtatctaa aatggggttt gccgatgcgg tcttcaaagt ctaaaattct 1440 tgaagtttgc gtttggtcgc aaaaattatg gatttagtcg attattgcct actaagcagc 1500 agtttagaag tttttaagat agttacgcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1558 <210> 61 <211> 1341 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 61 gtgagctact tatgaaacat gaatggtcgg tcttgtggtc gcttttgtcg aaatcaccgg 60 aaacattaaa tcaaagaagt ttatcaacca attagtacag tggcatgtta agtaaaacag 120 aaagaacgcc aagtggcaac aaatgacgta attatttaag caaaaagcgt aaacgcttac 180 aaaggctatt taaatatcag taaatcgttg gaaattgggt atgtgctgcc ttcatacgca 240 aaaatggatg cgacagaaac gagttgtatg gcctccacta cgctcgcctg ccgcggaccc 300 taaacgacag tcgtttgccc aactacggct acggcatcgc tcagaacttc gggtcttggc 360 ccacctttct gacgcctagt caacgtgcgt cgcctcagtc ttggacaaaa agaagaaata 420 tcaattgcaa aaataactat aggtacttct ttgaagaaac caaacgtgag tttctaacac 480 gactaaatcc atttgatctt tgagaaagtc gaagacgaat gaagtgcgta taattgatga 540 acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta gtgccgcttg aaaaagtaat 600 agtgcaaata agaaaaagaa gcgaaacggc attcgtttaa caaaacgtca catatttagg 660 tatagtagat tgcgttcaca aagaaattta aatttaattt accatttcgt cattgtatat 720 gcggtaaacg taaatttaca catatgacac gaactttgag ttttattagc ctgcctaatt 780 gttggtaaca atgtaatgga aattggcgat gactatgggg aaagagcaat caactcacca 840 taaatttgtg aacgcagtta agtaattggc atcgcgcgcc taatcgttta cattcgcatg 900 gaacaagaag aaagagacct attcgtggga gtcaatggct atgtggaaaa acatgaactg 960 ccaaattcgg ctaaccaacc agaaatggtc atggaaaatt cttgcgtcaa agttcgcggt 1020 gacgtttacg aagaagctaa acttgtgctt gtttagaaag ttaggtgcgt cctggtttta 1080 gtaaatttag aaaaccattt tgttgtttgc gacagttaaa taaaacttaa cgacaaagaa 1140 gtgacttgcg tcgtagatta actggaaatt aaagtggtat ctaaaatggg gtttgccgat 1200 gcggtcttca aagtctaaaa ttcttgaagt ttgcgtttgg tcgcaaaaat tatggattta 1260 gtcgattatt gcctactaag cagcagttta gaagttttta agatagttac gaaaaaaaaa 1320 aaaaaaaaaa aaaaaaaaaa a 1341 <210> 62 <211> 489 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 62 ggttcgtctt gaagcaatcg gtagtatctg cagtcaagtc gtagtcgtag gtatggcact 60 tgtcgtgcgt ctaggacgcg ttgaagctat cttattgccc gaaggagccg aaagtctggg 120 acacgtcgtg ggacgtatcg ttaggactat gggactagtc gtcttcggtc ttacaaatgt 180 tgcaatgccc tgtgagctac ttatgaaaca tgaatggtcg gtcttgtggt cgcttttgtc 240 gaaatcaccg gacaaaaaga agaaatatca attgcaaaaa taactatagg tacttctttg 300 aagaaaccaa acgtgagttt ctaacacgac taaatccatt tgatctttga gaaagtcgaa 360 gacgaatgaa gtgcgtataa ttgatgaacg agtcgaagtc gaagttgttc aacaagagag 420 ttaggtagtg ccgcttgaaa aagtaatagt gcaaataaga aaaaaaaaaa aaaaaaaaaa 480 aaaaaaaaa 489 <210> 63 <211> 813 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 63 ggggaaccaa tgctcagtgt tggtgaaaga atcgttaggt cgaactattg gtacgttcta 60 ataaaagacg acatagaaat tcgttacata atcaacgtca ctactaaggg cgtattgcaa 120 attagttcat aaattattaa cagtttgcac ctaatagttc tagtccttaa aaaggtagaa 180 gacgtaggta atgtcagttg cctcaatgta gtcaggtaaa aagtatatgt gctgatggca 240 aacttcatct ttgtgagagt tgagcatgta taacaacatc actcagtata accgcttggt 300 tacacaatta gtatcatgcc tatagaacgc cttatcattc aggtcgtcgt gatagtgaag 360 gggatcaaag tcaaaatcta ctttgtcata actctttacg gtatcccaat gcttggtata 420 agtgcttaag aaattgttta aacagaaact caagtataag cgaaaacaag ttacctggaa 480 aaagaagaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca acaagagagt 540 taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagaagcga aacggcattc 600 gtttaacaaa acgtcacata tttaggtata gtagattgcg ttcacaaaga aatttaaatt 660 taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata tgacacgaac 720 tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt ggcgatgact 780 atgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 813 <210> 64 <211> 561 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 64 gaatattgtt tcaatggtcg tggcgctggt cagactgctc gctaaagtcg tcgaagaaac 60 agctagacca tctattctct gaacttccta agtggtcggg ttggtcgtgg cgctcatagt 120 tagcccagaa cgtccagttt atgtggtggt gatcttggtt gccagactca gtatggtcaa 180 aatagttgtg ctcgccattt caactagtgc agttgctaaa gtgccaacta aggttcgtct 240 tgaagcaatc ggtagtatct gcagtcaagt cgtagtcgta ggtatggcac ttgtcgtgcg 300 tctaggacgc gttgaagcta tcttattgcc cgaaggagcc gaaagtctgg gacacgtcgt 360 gggacgtatc gttaggacta tgggactagt cgtcttcggt cttacaaatt gagctactta 420 tgaaacatga atggtcggtc ttgtggtcgc ttttgtcgaa atcaccgaag tgcgtataat 480 tgatgaacga gtcgaagtcg aagttgttca acaagagagt taggtagtgc caaaaaaaaa 540 aaaaaaaaaa aaaaaaaaaa a 561 <210> 65 <211> 306 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 65 gagatttcta cgttgctgac aaagaggatg accgtgacgc aaggtctttg agtgagacac 60 atgaggctaa cggcgtccac tataccatgt ggtgtggaag cgtctttgac cgtagcgtat 120 agacgttttg caatggcgtc ttgttcattg gggctggttt cgactgtcgc tcagtctact 180 ctttcaacag tacttcgact accttcttcc atttgaccga ctttagcgaa agccataccg 240 atgaaggtag cgactttccg tcgttcccaa attagtaaaa aaaaaaaaaa aaaaaaaaaa 300 aaaaaa 306 <210> 66 <211> 219 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 66 gcagatacta ccgattgctt caagacgaac cttagttggc actttagcaa ctgcactagt 60 tgaaatggcg agcacaacta ttttgaccat actgagtctg gcaaccaaga tcaccaccac 120 ataaactgga cgttctgggc taactatgag cgccacgacc aacccgacca cttaggaagt 180 tcagagaata aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 219 <210> 67 <211> 2492 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 67 gagacgatga aacttgagaa atcgcactgg cttacatata cacgataaaa gactttaata 60 caatataaga agtcgttatt gaagacggag atgtatgtta ccgcccaatt ttagagatac 120 aaaatgagac tgacatagcg gttaactata ttagagcacg cctaagcatt gaaggtttag 180 tcatcttcat tagagtatac tgtattgacg gctgaatgca aataaattga gacaaaaccg 240 atttcttgaa ttagcacgaa gtaagtataa aactagtaga gtgacaccaa gtatataggg 300 ggtttatatc gcgtggaatc gaacaacata tctatttaat tagtttaata acaaattaag 360 tgacttatcg cggaagagag aacaatagat tggattaaaa agttgtagta aaccacaacg 420 aagttaggta gctggtaagg ttatagatgc gttctgtcgt tgtagtccca atgaacatag 480 tatggtttcg catagtaact gacctaaaag accgccacat agtgaaaata cttaaaacgc 540 aagtttcata ggattaagtg gtagaggcta tagaaaatga tatcataact ataacgaata 600 taacagcaaa ttaaaatgca ctggaagtgg aaaatttcag caagagagtc gaagtcgtca 660 tagattagca tggataatgg caattatagt taaaatagtc gtaaattaga ttatggtgca 720 ccggggtgta agtttcgaac tcggtgaggc aatagtagta tagactaaca acaaggtaga 780 cattgcaatg tattgcgaga agaacttcac gaagatatag atacagtaca gaatttctag 840 gattaagtac agcagacgta tttacacctg ttataggata cttgttcact taatccctgt 900 tatgacaaag tagtctcata gaatttttag aacgagaaga tttgaatgga cgtaaaggaa 960 gaaggtcaaa atggagaact taaaagggga aatctagaac attgaaccca acattataca 1020 atgatataag aacgaaaaga cgaagacaat aaggtccatg ggatttaccc aaatgaggac 1080 ctaaatcacc gggaagaagt aagtaaacat aacgatatag ttttagatta tcgagaagtc 1140 gaagaagacc gggataggta acagataatc gacgtttggg aagacggtaa cgtaagaaac 1200 tgaggaggtt aaagaactaa gaatcaacaa caagaaacta ttgtccctat tctacttagg 1260 tatctgctag tatgcgatat ggaagttaaa gaggaaacag tagtatgcac tgtttgagaa 1320 aattaggatt taagaggtgg agaagtcgtt agtttggaag catagatagt tcaaatatcc 1380 acagaaggct attaaaagat agaagagagt cgagagatcg attttaaagt ctgagtcgaa 1440 atagtttggt gcaagattat ccattcaatt cttatcgaaa acttacaagg tcaacaagta 1500 gagatagcaa tataactaaa agctatctat tgaacataaa tgcgcgacta cagcttgcgg 1560 gttaacacgg tcgtcttcat tatacgttta atcgacgccg gcttattcat gcagatattt 1620 aactcaatct tcgataggat agaggacgta gtagacttgg atgtctgtca tccagacatg 1680 agacatatac ttatatggat aatggtagtg cagcaaagtc gtataattta aacggtatgg 1740 gacgaagcta acaacataga ggtggacaac gtatagcagg taattgttaa acttgttctg 1800 gaaataaaga aagaaatctt ttggactatg ggcgtggcat tagtatcaag agtagtcttg 1860 gtttgtatta tattggcagt gagcttattc gtaatagacg taatctgtcg cacttacgtt 1920 tagccaaatt aggtccgtga aacagacaca aacctatatt tatgtggcca cggacaaacg 1980 aaaactataa gctacttcgc attctgaggt cttgttgggc taataataga ctgcaatcac 2040 gttgctgggg tggtatcctt aactaacaat atggtaagta cggtgatagt gacattcaaa 2100 atagggtggt aacttagcat ggtctacttg gtggcagaag tggaagtggt agttatattc 2160 agctttatgg ttttacactg tatatttcac ttggcggtcg ccacaataaa gatttagaat 2220 ccactggcag aaagtcagcg catttttata gcagacaact tgcaaaggga gaggttcatg 2280 caacgtgtta tggtggatca agttatctta gtaagacata aacttgtata gacaataaca 2340 gtggcggaaa ttaaggttaa gataattgta actatagttc cgggagagaa ctatttaatt 2400 ccgacaactg ttatagccaa aatagatgaa atagagcgta taaaacctat tcttccaagc 2460 gtaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 2492 <210> 68 <211> 2277 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 68 gattggatta aaaagttgta gtaaaccaca acgaagttag gtagctggta aggttataga 60 tgcgttctgt cgttgtagtc ccaatgaaca tagtatggtt tcgcatagta actgacctaa 120 aagaccgcca catagtgaaa atacttaaaa cgcaagtttc ataggattaa gtggtagagg 180 ctatagaaaa tgatatcata actataacga atataacagc aaattaaaat gcactggaag 240 tggaaaattt cagcaagaga gtcgaagtcg tcatagatta gcatggacga aagaaaagtc 300 gcaaagatcg gaaatcaaga cgcaaaacaa aacggagaag ttcaaatcga actcgtagaa 360 catatcgctg gaacatatta aactcttggt atcgctcagt tacaaaagga gacacgtaga 420 cacgaaagaa actggaacaa cagacatatt tgtggataat ggcaattata gttaaaatag 480 tcgtaaatta gattatggtg caccggggtg taagtttcga actcggtgag gcaatagtag 540 tatagactaa caacaaggta gacattgcaa tgtattgcga gaagaacttc acgaagatat 600 agatacagta cagaatttct aggattaagt acagcagacg tatttacacc tgttatagga 660 tacttgttca cttaatccct gttatgacaa agtagtctca tagaattttt agaacgagaa 720 gatttgaatg gacgtaaagg aagaaggtca aaatggagaa cttaaaaggg gaaatctaga 780 acattgaacc caacattata caatgatata agaacgaaaa gacgaagaca ataaggtcca 840 tgggatttac ccaaatgagg acctaaatca ccgggaagaa gtaagtaaac ataacgatat 900 agttttagat tatcgagaag tcgaagaaga ccgggatagg taacagataa tcgacgtttg 960 ggaagacggt aacgtaagaa actgaggagg ttaaagaact aagaatcaac aacaagaaac 1020 tattgtccct attctactta ggtatctgct agtatgcgat atggaagtta aagaggaaac 1080 agtagtatgc actgtttgag aaaattagga tttaagaggt ggagaagtcg ttagtttgga 1140 agcatagata gttcaaatat ccacagaagg ctattaaaag atagaagaga gtcgagagat 1200 cgattttaaa gtctgagtcg aaatagtttg gtgcaagatt atccattcaa ttcttatcga 1260 aaacttacaa ggtcaacaag tagagatagc aatataacta aaagctatct attgaacata 1320 aatgcgcgac tacagcttgc gggttaacac ggtcgtcttc attatacgtt taatcgacgc 1380 cggcttattc atgcagatat ttaactcaat cttcgatagg atagaggacg tagtagactt 1440 ggatgtctgt catccagaca tgagacatat acttatatgg ataatggtag tgcagcaaag 1500 tcgtataatt taaacggtat gggacgaagc taacaacata gaggtggaca acgtatagca 1560 ggtaattgtt aaacttgttc tggaaataaa gaaagaaatc ttttggacta tgggcgtggc 1620 attagtatca agagtagtct tggtttgtat tatattggca gtgagcttat tcgtaataga 1680 cgtaatctgt cgcacttacg tttagccaaa ttaggtccgt gaaacagaca caaacctata 1740 tttatgtggc cacggacaaa cgaaaactat aagctacttc gcattctgag gtcttgttgg 1800 gctaataata gactgcaatc acgttgctgg ggtggtatcc ttaactaaca atatggtaag 1860 tacggtgata gtgacattca aaatagggtg gtaacttagc atggtctact tggtggcaga 1920 agtggaagtg gtagttatat tcagctttat ggttttacac tgtatatttc acttggcggt 1980 cgccacaata aagatttaga atccactggc agaaagtcag cgcattttta tagcagacaa 2040 cttgcaaagg gagaggttca tgcaacgtgt tatggtggat caagttatct tagtaagaca 2100 taaacttgta tagacaataa cagtggcgga aattaaggtt aagataattg taactatagt 2160 tccgggagag aactatttaa ttccgacaac tgttatagcc aaaatagatg aaatagagcg 2220 tataaaacct attcttccaa gcgtaataaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 2277 <210> 69 <211> 2528 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 69 gatgaaactt gagaaatcgc actggcttac atatacacga taaaagactt taatacaata 60 taagaagtcg ttattgaaga cggagatgta tgttaccgcc caattttaga gatacaaaat 120 gagactgaca tagcggttaa ctatattaga gcacgcctaa gcattgaagg tttagtcatc 180 ttcattagag tatactgtat tgacggctga atgcaaataa attgagacaa aaccgatttc 240 ttgaattagc acgaagtaag tataaaacta gtagagtgac accaagtata tagggggttt 300 atatcgcgtg gaatcgaaca acatatctat ttaattagtt taataacaaa ttaagtgact 360 tatcgcggaa gagagaacaa tagattggat taaaaagttg tagtaaacca caacgaagtt 420 aggtagctgg taaggttata gatgcgttct gtcgttgtag tcccaatgaa catagtatgg 480 tttcgcatag taactgacct aaaagaccgc cacatagtga aaatacttaa aacgcaagtt 540 tcataggatt aagtggtaga ggctatagaa aatgatatca taactataac gaatataaca 600 gcaaattaaa atgcactgga tgttagagca aattatataa actttcacat gctaaccaac 660 gtaatttgag aagaaatcat caagaattaa actaaaggaa tggtggataa tggcaattat 720 agttaaaata gtcgtaaatt agattatggt gcaccggggt gtaagtttcg aactcggtga 780 ggcaatagta gtatagacta acaacaaggt agacattgca atgtattgcg agaagaactt 840 cacgaagata tagatacagt acagaatttc taggattaag tacagcagac gtatttacac 900 ctgttatagg atacttgttc acttaatccc tgttatgaca aagtagtctc atagaatttt 960 tagaacgaga agatttgaat ggacgtaaag gaagaaggtc aaaatggaga acttaaaagg 1020 ggaaatctag aacattgaac ccaacattat acaatgatat aagaacgaaa agacgaagac 1080 aataaggtcc atgggattta cccaaatgag gacctaaatc accgggaaga agtaagtaaa 1140 cataacgata tagttttaga ttatcgagaa gtcgaagaag accgggatag gtaacagata 1200 atcgacgttt gggaagacgg taacgtaaga aactgaggag gttaaagaac taagaatcaa 1260 caacaagaaa ctattgtccc tattctactt aggtatctgc tagtatgcga tatggaagtt 1320 aaagaggaaa cagtagtatg cactgtttga gaaaattagg atttaagagg tggagaagtc 1380 gttagtttgg aagcatagat agttcaaata tccacagaag gctattaaaa gatagaagag 1440 agtcgagaga tcgattttaa agtctgagtc gaaatagttt ggtgcaagat tatccattca 1500 attcttatcg aaaacttaca aggtcaacaa gtagagatag caatataact aaaagctatc 1560 tattgaacat aaatgcgcga ctacagcttg cgggttaaca cggtcgtctt cattatacgt 1620 ttaatcgacg ccggcttatt catgcagata tttaactcaa tcttcgatag gatagaggac 1680 gtagtagact tggatgtctg tcatccagac atgagacata tacttatatg gataatggta 1740 gtgcagcaaa gtcgtataat ttaaacggta tgggacgaag ctaacaacat agaggtggac 1800 aacgtatagc aggtaattgt taaacttgtt ctggaaataa agaaagaaat cttttggact 1860 atgggcgtgg cattagtatc aagagtagtc ttggtttgta ttatattggc agtgagctta 1920 ttcgtaatag acgtaatctg tcgcacttac gtttagccaa attaggtccg tgaaacagac 1980 acaaacctat atttatgtgg ccacggacaa acgaaaacta taagctactt cgcattctga 2040 ggtcttgttg ggctaataat agactgcaat cacgttgctg gggtggtatc cttaactaac 2100 aatatggtaa gtacggtgat agtgacattc aaaatagggt ggtaacttag catggtctac 2160 ttggtggcag aagtggaagt ggtagttata ttcagcttta tggttttaca ctgtatattt 2220 cacttggcgg tcgccacaat aaagatttag aatccactgg cagaaagtca gcgcattttt 2280 atagcagaca acttgcaaag ggagaggttc atgcaacgtg ttatggtgga tcaagttatc 2340 ttagtaagac ataaacttgt atagacaata acagtggcgg aaattaaggt taagataatt 2400 gtaactatag ttccgggaga gaactattta attccgacaa ctgttatagc caaaatagat 2460 gaaatagagc gtataaaacc tattcttcca agcgtaataa aaaaaaaaaa aaaaaaaaaa 2520 aaaaaaaa 2528 <210> 70 <211> 458 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 70 ggctatagaa aatgatatca taactataac gaatataaca gcaaattaaa atgcactgga 60 taataaaaat taaactaata tttgtagtta gtggtgcaga tttgaacgaa cagtcaggcg 120 aaatggaagt aatagatgat tcatggacga taagtcatta agacatcgaa gactgttttg 180 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 240 aaaaatccta agagatcgac gaagttatcg actaaattgt tgtaatccaa gaggctgatt 300 gacgtttaga agtaagtggc aatttggttt aaaacaacga taataaaggt tgcgaagaaa 360 tataaaaagt agtcacataa cactgacatt attaaattta gatagacttt accgctaaaa 420 ctgtagttaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 458 <210> 71 <211> 2492 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 71 gtgagacgat gaaacttgag aaatcgcact ggcttacata tacacgataa aagactttaa 60 tacaatataa gaagtcgtta ttgaagacgg agatgtatgt taccgcccaa ttttagagat 120 acaaaatgag actgacatag cggttaacta tattagagca cgcctaagca ttgaaggttt 180 agtcatcttc attagagtat actgtattga cggctgaatg caaataaatt gagacaaaac 240 cgatttcttg aattagcacg aagtaagtat aaaactagta gagtgacacc aagtatatag 300 ggggtttata tcgcgtggaa tcgaacaaca tatctattta attagtttaa taacaaatta 360 agtgacttat cgcggaagag agaacaatag attggattaa aaagttgtag taaaccacaa 420 cgaagttagg tagctggtaa ggttatagat gcgttctgtc gttgtagtcc caatgaacat 480 agtatggttt cgcatagtaa ctgacctaaa agaccgccac atagtgaaaa tacttaaaac 540 gcaagtttca taggattaag tggtagaggc tatagaaaat gatatcataa ctataacgaa 600 tataacagca aattaaaatg cactggaagt ggaaaatttc agcaagagag tcgaagtcgt 660 catagattag catggataat ggcaattata gttaaaatag tcgtaaatta gattatggtg 720 caccggggtg taagtttcga actcggtgag gcaatagtag tatagactaa caacaaggta 780 gacattgcaa tgtattgcga gaagaacttc acgaagatat agatacagta cagaatttct 840 aggattaagt acagcagacg tatttacacc tgttatagga tacttgttca cttaatccct 900 gttatgacaa agtagtctca tagaattttt agaacgagaa gatttgaatg gacgtaaagg 960 aagaaggtca aaatggagaa cttaaaaggg gaaatctaga acattgaacc caacattata 1020 caatgatata agaacgaaaa gacgaagaca ataaggtcca tgggatttac ccaaatgagg 1080 acctaaatca ccgggaagaa gtaagtaaac ataacgatat agttttagat tatcgagaag 1140 tcgaagaaga ccgggatagg taacagataa tcgacgtttg ggaagacggt aacgtaagaa 1200 actgaggagg ttaaagaact aagaatcaac aacaagaaac tattgtccct attctactta 1260 ggtatctgct agtatgcgat atggaagtta aagaggaaac agtagtatgc actgtttgag 1320 aaaattagga tttaagaggt ggagaagtcg ttagtttgga agcatagata gttcaaatat 1380 ccacagaagg ctattaaaag atagaagaga gtcgagagat cgattttaaa gtctgagtcg 1440 aaatagtttg gtgcaagatt atccattcaa ttcttatcga aaacttacaa ggtcaacaag 1500 tagagatagc aatataacta aaagctatct attgaacata aatgcgcgac tacagcttgc 1560 gggttaacac ggtcgtcttc attatacgtt taatcgacgc cggcttattc atgcagatat 1620 ttaactcaat cttcgatagg atagaggacg tagtagactt ggatgtctgt catccagaca 1680 tgagacatat acttatatgg ataatggtag tgcagcaaag tcgtataatt taaacggtat 1740 gggacgaagc taacaacata gaggtggaca acgtatagca ggtaattgtt aaacttgttc 1800 tggaaataaa gaaagaaatc ttttggacta tgggcgtggc attagtatca agagtagtct 1860 tggtttgtat tatattggca gtgagcttat tcgtaataga cgtaatctgt cgcacttacg 1920 tttagccaaa ttaggtccgt gaaacagaca caaacctata tttatgtggc cacggacaaa 1980 cgaaaactat aagctacttc gcattctgag gtcttgttgg gctaataata gactgcaatc 2040 acgttgctgg ggtggtatcc ttaactaaca atatggtaag tacggtgata gtgacattca 2100 aaatagggtg gtaacttagc atggtctact tggtggcaga agtggaagtg gtagttatat 2160 tcagctttat ggttttacac tgtatatttc acttggcggt cgccacaata aagatttaga 2220 atccactggc agaaagtcag cgcattttta tagcagacaa cttgcaaagg gagaggttca 2280 tgcaacgtgt tatggtggat caagttatct tagtaagaca taaacttgta tagacaataa 2340 cagtggcgga aattaaggtt aagataattg taactatagt tccgggagag aactatttaa 2400 ttccgacaac tgttatagcc aaaatagatg aaatagagcg tataaaacct attcttccaa 2460 gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 2492 <210> 72 <211> 979 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 72 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtgg aatcgaacaa 360 catatctatt taattagttt aataacaaat taagtgactt atcgcggaag agagaacaat 420 agattggatt aaaaagttgt agtaaaccac aacgaagtta ggtagctggt aaggttatag 480 atgcgttctg tcgttgtagt cccaatgaac atagtatggt ttcgcatagt aactgaccta 540 aaagaccgcc acatagtgaa aatacttaaa acgcaagttt cataggatta agtggtagag 600 gctatagaaa atgatatcat aactataacg aatataacag caaattaaaa tgcactggat 660 aataaaaatt aaactaatat ttgtagttag tggtgcagat ttgaacgaac agtcaggcga 720 aatggaagta atagatgatt catggacgat aagtcattaa gacatcgaag actgttttgg 780 atgtagacat atttgtagaa cattaaagaa agatatgagt tctttatttc aaaaacatag 840 atggttggct ggttagcaac gtcaaagccc acagtatcta atgttgttat ggcagaaaca 900 gacaaaatat caggctacaa aaatgtatca gagcattaac atgaaaaata aaaaaaaaaa 960 aaaaaaaaaa aaaaaaaaa 979 <210> 73 <211> 2356 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 73 gcactggctt acatatacac gataaaagac tttaatacaa tataagaagt cgttattgaa 60 gacggagatg tatgttaccg cccaatttta gagatacaaa atgagactga catagcggtt 120 aactatatta gagcacgcct aagcattgaa ggtttagtca tcttcattag agtatactgt 180 attgacggct gaatgcaaat aaattgagac aaaaccgatt tcttgaatta gcacgaagta 240 agtataaaac tagtagagtg acaccaagta tatagggggt ttatatcgcg tggataataa 300 aaattaaact aatatttgta gttagtggtg cagatttgaa cgaacagtca ggcgaaatgg 360 aagtaataga tgattcatgg acgataagtc attaagacat cgaagactgt tttggaagtg 420 gaaaatttca gcaagagagt cgaagtcgtc atagattagc atggatttcg aagaacgcga 480 agaactaaac gaacaagtta tgaacgaaat tagagtccta ataacctgtt tagtgaattg 540 accagtagtc atataacaaa aacaagaagg ttaggcaaag ctataagcaa atagcattga 600 aatagaaatg gacgagaaga aggctttaaa atggtagtcg tatgcgaacg ctaaggaaga 660 gaaattctag tagaactcgt agttacatac ttggacgaaa gaaaagtcgc aaagatcgga 720 aatcaagacg caaaacaaaa cggagaagtt caaatcgaac tcgtagaaca tatcgctgga 780 acatattaaa ctcttggtat cgctcagtta caaaaggaga cacgtagaca cgaaagaaac 840 tggaacaaca gacatatttg tggatgttag agcaaattat ataaactttc acatgctaac 900 caacgtaatt tgagaagaaa tcatcaagaa ttaaactaaa ggaatggtgg ataatggcaa 960 ttatagttaa aatagtcgta aattagatta tggtgcaccg gggtgtaagt ttcgaactcg 1020 gtgaggcaat agtagtatag actaacaaca aggtagacat tgcaatgtat tgcgagaaga 1080 acttcacgaa gatatagata cagtacagaa tttctaggat taagtacagc agacgtattt 1140 acacctgtta taggatactt gttcacttaa tccctgttat gacaaagtag tctcatagaa 1200 tttttagaac gagaagattt gaatggacgt aaaggaagaa ggtcaaaatg gagaacttaa 1260 aaggggaaat ctagaacatt gaacccaaca ttatacaatg atataagaac gaaaagacga 1320 agacaataag gtccatggga tttacccaaa tgaggaccta aatcaccggg aagaagtaag 1380 taaacataac gatatagttt tagattatcg agaagtcgaa gaagaccggg ataggtaaca 1440 gataatcgac gtttgggaag acggtaacgt aagaaactga ggaggttaaa gaactaagaa 1500 tcaacaacaa gaaactattg tccctattct acttaggtat ctgctagtat gcgatatgga 1560 agttaaagag gaaacagtag tatgcactgt ttgagaaaat taggatttaa gaggtggaga 1620 agtcgttagt ttggaagcat agatagttca aatatccaca gaaggctatt aaaagataga 1680 agagagtcga gagatcgatt ttaaagtctg agtcgaaata gtttggtgca agattatcca 1740 ttcaattctt atcgaaaact tacaaggtca acaagtagag atagcaatat aactaaaagc 1800 tatctattga acataaatgc gcgactacag cttgcgggtt aacacggtcg tcttcattat 1860 acgtttaatc gacgccggct tattcatgca gatatttaac tcaatcttcg ataggataga 1920 ggacgtagta gacttggatg tctgtcatcc agacatgaga catatactta tatggataat 1980 ggtagtgcag caaagtcgta taatttaaac ggtatgggac gaagctaaca acatagaggt 2040 ggacaacgta tagcaggtaa ttgttaaact tgttctggaa ataaagaaag aaatcttttg 2100 gactatgggc gtggcattag tatcaagagt agtcttggtt tgtattatat tggcagtgag 2160 cttattcgta atagacgtaa tctgtcgcac ttacgtttag ccaaattagg tccgtgaaac 2220 agacacaaac ctatatttat gtggccacgg acaaacgaaa actataagct acttcgcatt 2280 ctgaggtctt gttgggctaa taatagactg caatcacgtt gctgggaaaa aaaaaaaaaa 2340 aaaaaaaaaa aaaaaa 2356 <210> 74 <211> 919 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 74 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtgt ttaataacaa 360 attaagtgac ttatcgcgga agagagaaca atagattgga ttaaaaagtt gtagtaaacc 420 acaacgaagt taggtagctg gtaaggttat agatgcgttc tgtcgttgta gtcccaatga 480 acatagtatg gtttcgcata gtaactgacc taaaagaccg ccacatagtg aaaatactta 540 aaacgcaagt ttcataggat taagtggtag aggctataga aaatgatatc ataactataa 600 cgaatataac agcaaattaa aatgataata aaaattaaac taatatttgt agttagtgtc 660 aggcgaaatg gaagtaatag atgattcatg gacgataagt cattaagaca tcgaagactg 720 ttttggatgt agacatattt gtagaacatt aaagaaagat atgagttctt tatttcaaaa 780 acatagatgg ttggctggtt agcaacgtca aagcccacag tatctaatgt tgttatggca 840 gaaacagaca aaatatcagg ctacaaaaat gtatcagagc attaacatga aaaaaaaaaa 900 aaaaaaaaaa aaaaaaaaa 919 <210> 75 <211> 1561 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 75 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acagctatcc 240 tgaggtgcca ggttagaata ggccctacca catctaagcc ttgaaacggt ctgttcctcg 300 tgtcaagttc tttgtccgct tataccgcca tctctcgttg tggtacaaga gttggttact 360 ctcagtccca ttcccaacac atcactgaga agtatagagc ctagtggtca ttagaaggtc 420 aacatggagt aggtgtttag gtgtgtcgga gggcagaaac caacgaaaga aaggaaacga 480 tcaacactta tatagtgtcc tgtcgggccc aaatcctaag agaccttagg tttaccacat 540 tagtactaaa cagcctgttg tataccgtga gcccccactt cgatctgtaa atggtaatcc 600 cttgtaaaac ctcatctcca aaaacagtat acgcaggtac agctgatgtg atcctctcta 660 ctagtcttct ctgaggaacc atgagatgga tagtggggga cctgagtcat actagggtaa 720 cttcttatat ggtgacatca ctcctaccgt agagggggga cggtagtctg tgggaaatct 780 accctagtga agaaaggtaa gagaatgtct gaacattacc tgtagacgaa ggtactctca 840 gaacaactac tctcacatac ggaaaggttt gggtccttgg tcaaaggact ttactgactc 900 tgcagacgat gaatatctga accaacacta ctatcaaaga tgttcgacta gacctctcgt 960 ggtagggcct ggaagtcaaa agacttctct atgggtacta ggtaggtagt tagagtacgg 1020 tccctggggt gtctcagagt cgaacgttcg ggggaagttc ctaaacatgt taggatgtta 1080 tgataacaag tccaaacgtg gacgtcgtcg ctgtcacctc tcggaacact agctgaggtg 1140 gcttaaaaac attccgtaac tgagtggtca ccgggggtga aacatgctga gggttctacc 1200 ttaacgggaa ggtgaaactc tccaatgtct ttgaaaggac tagttgagat aacccaaacc 1260 gaatccggag gaagaggaga acttggaaaa cgttgtacga cgtcattaat aacgattccg 1320 ttcttcccgt tcgtgtctat aaggatattc tttcattgcg aaggtaagcc cttttgggtc 1380 tagatacgga ctatactcaa aaacccacag accttccact tggagttgtt ttaggagacg 1440 gagtagtaga gaaaacttgt ggcaccttcc cagtcggttc cctacagact ctccttagta 1500 gctcctaaga tcgtcgagtt acacgacgac attgttccga ctgacgtaga ggcattactt 1560 t 1561 <210> 76 <211> 1300 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 76 gtacaagagt tggttactct cagtcccatt cccaacacat cactgagaag tatagagcct 60 agtggtcatt agaaggtcaa catggagtag gtgtttaggt gtgtcggagg gcagaaacca 120 acgaaagaaa ggaaacgatc aacacttata tagtgtcctg tcgggcccaa atcctaagag 180 accttaggtt taccacatta gtactaaaca gcctgttgta taccgtgagc ccccacttcg 240 atctgtaaat ggtaatccct tgtaaaacct catctccaaa aacagtatac gcaggtacag 300 ctgatgtgat cctctctact agtcttctct gaggaaccat gagatggata gtgggggacc 360 tgagtcatac tagggtaact tcttatatgg tgacatcact cctaccgtag aggggggacg 420 gtagtctgtg ggaaatctac cctagtgaag aaaggtaaga gaatgtctga acattacctg 480 tagacgaagg tactctcaga acaactactc tcacatacgg aaaggtttgg gtccttggtc 540 aaaggacttt actgactctg cagacgatga atatctgaac caacactact atcaaagatg 600 tcgtaggttt gtgagaaggg agaagaagtg gttgagaagt tggtgttcca caagttaaag 660 tagacatacc tttagtacct gtaaaggttc gactagacct ctcgtggtag ggcctggaag 720 tcaaaagact tctctatggg tactaggtag gtagttagag tacggtccct ggggtgtctc 780 agagtcgaac gttcggggga agttcctaaa catgttagga tgttatgata acaagtccaa 840 acgtggacgt cgtcgctgtc acctctcgga acactagctg aggtggctta aaaacattcc 900 gtaactgagt ggtcaccggg ggtgaaacat gctgagggtt ctaccttaac gggaaggtga 960 aactctccaa tgtctttgaa aggactagtt gagataaccc aaaccgaatc cggaggaaga 1020 ggagaacttg gaaaacgttg tacgacgtca ttaataacga ttccgttctt cccgttcgtg 1080 tctataagga tattctttca ttgcgaaggt aagccctttt gggtctagat acggactata 1140 ctcaaaaacc cacagacctt ccacttggag ttgttttagg agacggagta gtagagaaaa 1200 cttgtggcac cttcccagtc ggttccctac agactctcct tagtagctcc taagatcgtc 1260 gagttacacg acgacattgt tccgactgac gtagaggcat 1300 <210> 77 <211> 1363 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 77 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 120 agataaaaat aacaacaaca gaaggtacaa gagttggtta ctctcagtcc cattcccaac 180 acatcactga gaagtataga gcctagtggt cattagaagg tcaacatgga gtaggtgttt 240 aggtgtgtcg gagggcagaa accaacgaaa gaaaggaaac gatcaacact tatatagtgt 300 cctgtcgggc ccaaatccta agagacctta ggtttaccac attagtacta aacagcctgt 360 tgtataccgt gagcccccac ttcgatctgt aaatggtaat cccttgtaaa acctcatctc 420 caaaaacagt atacgcaggt acagctgatg tgatcctctc tactagtctt ctctgaggaa 480 ccatgagatg gatagtgggg gacctgagtc atactagggt aacttcttat atggtgacat 540 cactcctacc gtagaggggg gacggtagtc tgtgggaaat ctaccctagt gaagaaaggt 600 aagagaatgt ctgaacatta cctgtagacg aaggtactct cagaacaact actctcacat 660 acggaaaggt ttgggtcctt ggtcaaagga ctttactgac tctgcagacg atgaatatct 720 gaaccaacac tactatcaaa gatgttcgac tagacctctc gtggtagggc ctggaagtca 780 aaagacttct ctatgggtac taggtaggta gttagagtac ggtccctggg gtgtctcaga 840 gtcgaacgtt cgggggaagt tcctaaacat gttaggatgt tatgataaca agtccaaacg 900 tggacgtcgt cgctgtcacc tctcggaaca ctagctgagg tggcttaaaa acattccgta 960 actgagtggt caccgggggt gaaacatgct gagggttcta ccttaacggg aaggtgaaac 1020 tctccaatgt ctttgaaagg actagttgag ataacccaaa ccgaatccgg aggaagagga 1080 gaacttggaa aacgttgtac gacgtcatta ataacgattc cgttcttccc gttcgtgtct 1140 ataaggatat tctttcattg cgaaggtaag cccttttggg tctagatacg gactatactc 1200 aaaaacccac agaccttcca cttggagttg ttttaggaga cggagtagta gagaaaactt 1260 gtggcacctt cccagtcggt tccctacaga ctctccttag tagctcctaa gatcgtcgag 1320 ttacacgacg acattgttcc gactgacgta gaggcattac ttt 1363 <210> 78 <211> 1399 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 78 gttttacaaa ggagaacttc caacatcagt gtatgggatg tttcaaacac ccccagagac 60 ggagacaata gtgtggtcac gtacatttag gaagataaaa ataacaacaa cagaagcctt 120 gaaacggtct gttcctcgtg tcaagttctt tgtccgctta taccgccatc tctcgttgtg 180 gtacaagagt tggttactct cagtcccatt cccaacacat cactgagaag tatagagcct 240 agtggtcatt agaaggtcaa catggagtag gtgtttaggt gtgtcggagg gcagaaacca 300 acgaaagaaa ggaaacgatc aacacttata tagtgtcctg tcgggcccaa atcctaagag 360 accttaggtt taccacatta gtactaaaca gcctgttgta taccgtgagc ccccacttcg 420 atctgtaaat ggtaatccct tgtaaaacct catctccaaa aacagtatac gcaggtacag 480 ctgatgtgat cctctctact agtcttctct gaggaaccat gagatggata gtgggggacc 540 tgagtcatac tagggtaact tcttatatgg tgacatcact cctaccgtag aggggggacg 600 gtagtctgtg ggaaatctac cctagtgaag aaaggtaaga gaatgtctga acattacctg 660 tagacgaagg tactctcaga acaactactc tcacatacgg aaaggtttgg gtccttggtc 720 aaaggacttt actgactctg cagacgatga atatctgaac caacactact atcaaagatg 780 ttcgactaga cctctcgtgg tagggcctgg aagtcaaaag acttctctat gggtactagg 840 taggtagtta gagtacggtc cctggggtgt ctcagagtcg aacgttcggg ggaagttcct 900 aaacatgtta ggatgttatg ataacaagtc caaacgtgga cgtcgtcgct gtcacctctc 960 ggaacactag ctgaggtggc ttaaaaacat tccgtaactg agtggtcacc gggggtgaaa 1020 catgctgagg gttctacctt aacgggaagg tgaaactctc caatgtcttt gaaaggacta 1080 gttgagataa cccaaaccga atccggagga agaggagaac ttggaaaacg ttgtacgacg 1140 tcattaataa cgattccgtt cttcccgttc gtgtctataa ggatattctt tcattgcgaa 1200 ggtaagccct tttgggtcta gatacggact atactcaaaa acccacagac cttccacttg 1260 gagttgtttt aggagacgga gtagtagaga aaacttgtgg caccttccca gtcggttccc 1320 tacagactct ccttagtagc tcctaagatc gtcgagttac acgacgacat tgttccgact 1380 gacgtagagg cattacttt 1399 <210> 79 <211> 670 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 79 gcgcaaggta catgctctta ccccactctc agcacgtagg gagcggagta caagagttgg 60 ttactctcag tcccattccc aacacatcac tgagaagtat agagcctagt ggtcattaga 120 aggtcaacat ggagtaggtg tttaggtgtg tcggagggca gaaaccaacg aaagaaagga 180 aacgatcaac acttatatag tgtcctgtcg ggcccaaatc ctaagagacc ttaggtttac 240 cacattagta ctaaacagcc tgttgtatac cgtgagcccc cacttcgatc tgtaaatggt 300 aatcccttgt aaaacctcat ctccaaaaac agtatacgca ggtacagctg atgtgatcct 360 ctctactagt cttctctgag gaaccatgag atggatagtg ggggacctga gtcatactag 420 ggtaacttct tatatggtga catcactcct accgtagagg ggggacggta gtctgtggga 480 aatctaccct agtgaagaaa ggtaagagaa tgtctgaaca ttacctgtag acgaaggtac 540 tctcagaaca actactctca catacggaaa ggtttgggtc cttggtcaaa ggactttact 600 gactctgcag acgatgaata tctgaaccaa cactactatc aaagatgttc gactagacct 660 ctcgtggtag 670 <210> 80 <211> 973 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 80 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acagtgttta 240 ggtgtgtcgg agggcagaaa ccaacgaaag aaaggaaacg atcaacactt atatagtgtc 300 ctgtcgggcc caaatcctaa gagaccttag gtttaccaca ttagtactaa acagcctgtt 360 gtataccgtg agcccccact tcgatctgta aatggtaatc ccttgtaaaa cctcatctcc 420 aaaaacagta tacgcaggta cagctgatgt gatcctctct actagtcttc tctgaggaac 480 catgagatgt tatgataaca agtccaaacg tggacgtcgt cgctgtcacc tctcggaaca 540 ctagctgagg tggcttaaaa acattccgta actgagtggt caccgggggt gaaacatgct 600 gagggttcta ccttaacggg aaggtgaaac tctccaatgt ctttgaaagg actagttgag 660 ataacccaaa ccgaatccgg aggaagagga gaacttggaa aacgttgtac gacgtcatta 720 ataacgattc cgttcttccc gttcgtgtct ataaggatat tctttcattg cgaaggtaag 780 cccttttggg tctagatacg gactatactc aaaaacccac agaccttcca cttggagttg 840 ttttaggaga cggagtagta gagaaaactt gtggcacctt cccagtcggt tccctacaga 900 ctctccttag tagctcctaa gatcgtcgag ttacacgacg acattgttcc gactgacgta 960 gaggcattac ttt 973 <210> 81 <211> 744 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 81 gtgaggcatc tataccgcca cgccctatgt ctttctcacg tataggtgtg ctctcagctg 60 ccgttccggc gctgcttcac ccctttccgc ttatcgcgga tggtaaagtc aaagtttact 120 cagcggtcgc tgttgacctc aggttctcta tcaaaaattt gctggctaaa acggtcggag 180 cttctgctcc actctttcca gcaatccttc tgccagtcgc cttcaaactt gcaatctgtg 240 gattcgtcga acgtccgggt agtatagtta tctgcatcgt ggtcaccagt atcacagact 300 acaaaatctc aatcctccat tcccccgtca ggtctatcta aaattcaatc gctgtgtttg 360 cggggtaaat aataggacaa gtccgtataa tctacccaat cgcattcatt ccagtcagag 420 ggtaccaccg aatttcatca acaacattaa taattccata ggaactacat tcttaaatcc 480 ttggaactcc aaactcctac cttatcacgg ttctatccgt cgtcttaggg tcggtctcct 540 cgtagcatgg cctcgttctc ctcgccgtaa ttgcggtcga aagtacttca gttatttatg 600 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 660 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 720 agataaaaat aacaacaaca gaag 744 <210> 82 <211> 702 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 82 gtcttccaca ctccgctccc tacgtgctga gagtggggta agagcatgta ccttgcgcct 60 cacaccttct gttgttgtta tttttatctt cctaaatgta cgtgaccaca ctattgtctc 120 cgtctctggg ggtgtttgaa acatcccata cactgatgtt ggaagttctc ctttgtaaaa 180 cccggttgac actcgcggac aagcctcact actttcgacc gcaattacgg cgaggagaac 240 gaggccatgc tacgaggaga ccgaccctaa gacgacggat agaaccgtga taaggtagga 300 gtttggagtt ccaaggattt aagaatgtag ttcctatgga attattaatg ttgttgatga 360 aattcggtgg taccctctga ctggaatgaa tgcgattggg tagattatac ggacttgtcc 420 tattatttac cccgcaaaca cagcgattga attttagata gacctgacgg gggaatggag 480 gattgagatt ttgtagtctc acagattgca agtttgaagg cgactggcag aaggattgct 540 ggaaagagtg gagcagaagc tccgaccgtt ttagccagca aatttttgat agagaacctg 600 aggtcaacag cgaccgctga gtaaactttg actttaccat ccgcgataag cggaaagggg 660 tgaagcagcg ccggaacggc agctgagagc acacctatac gt 702 <210> 83 <211> 464 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 83 gggtgtttga aacatcccat acactgatgt tggaagttct cctttgtaaa acccggttga 60 cactcgcgga caagcctcac cataaataac tgaagtactt tcgaccgcaa ttacggcgag 120 gagaacgagg ccatgctacg aggagaccga ccctaagacg acggatagaa ccgtgataag 180 gtaggagttt ggagttccaa ggatttaaga atgtagttcc tatggaatta ttaatgttgt 240 tgatgaaatt cggtgaagga ttgctggaaa gagtggagca gaagctccga ccgttttagc 300 cagcaaattt ttgatagaga acctgaggtc aacagcgacc gctgagtaaa ctttgacttt 360 accatccgcg ataagcggaa aggggtgaag cagcgccgga acggcagctg agagcacacc 420 tatacgtgag aaagacatag ggcgtggcgg tatagatgcc tcac 464 <210> 84 <211> 2051 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 84 gtgtatttat ccgctacgtg gtggatgaac atggatgcga acgacatttc gaaatatacg 60 caatgatatc caaccctacc gaaacagcaa taattgtaga aggtagcata tcatgccgag 120 agtttactac gttgtagcga gtttagttga aatagtttgt gatgtgtaaa ctaaaacata 180 ccaaatggta aaacttgtca cccccattta cattgcgaca gacacagtta tggagtggat 240 ggaagtgtgt tcgactgtca agacccttct ataatagtcg aaccagtacg gtaagtgggc 300 aacatgccca tataagtcga agcatttcaa atggttcatc cagtagacga cgatgtccta 360 gacagtcatg tctcttcccc atttgaagtc atgtagtggt tgtctacttt ttcattgaag 420 acgtccacct caacgctatg aacgttagtt gagacaatgt taaagcaaac gacaacgtca 480 ttgtgatcta catacacagt ggatggttag cgtaaacatg gtggaacgta ctttgactgc 540 gctgcaagaa atggttgggt cgatcgtaat gctagtcgtt acatcggaac aagccaaaac 600 aaatcattcg ctggatttag acctactgca cgacgacgtc gacacaagac attcttgaaa 660 ggaattcaat gcagaaactc ctatgcacgt cgaactagtc tcttgctcag cgctagtcca 720 tgaaaatgtc ctagctttga acgtttatca aatgcaagtt gtgggagtta gtcatgtgag 780 agttgtgcaa gaaagtgtta acgttctagc cgtgctcagt aacaattacc attcggcggt 840 aacaaacgta agttgtcact gcactcatga gcatgaagtt agaccagtag ttgggtcaga 900 aattagacgt ggtttagtct ggttaccgtg gaaagaatag ctgaactcaa agaagccgac 960 aaagataaac aaagaagaaa cggttaatac gattgaggag aaatattcaa tccaacggca 1020 actattaacg tttcaactag aatctacgtt tcatacgcgg attccacatc cgttcttgca 1080 attaaagaaa cgatgtagaa aacgctttgg ttggcgacaa cttgggcggc aaaataatta 1140 tggtaggtaa ttgaagtagc gttaaaacgc aaaaagaagg aagcacggat cactgcaaaa 1200 aggactactc aataatggtt tctaaagtca ttatgctggt gctccctatg gcggtggtca 1260 gtactgtaac taaagatgga ttacggcact tgtagagcac gtatcagacc cagctttgaa 1320 agaaggctga gttcggttcg ggtttcacgt aggcctagaa cgaggtatag aagtggttaa 1380 caaagatttg gttaccatgt aagaagcggt agacgccatg gcctagtagg aagaatgctt 1440 gttcacggag ccgacgaagt tagacgagtg gcagaagaaa gagcattatc ttggtactcg 1500 gactgaatgc gtactacggt cgtccaagtt gcaagaggta ttagagtcat tcgaatagct 1560 gtgggagtgg tatttatagc ataataagtg taaaaaacat tcccaacagc atatgtgtag 1620 ccaagaagcg ttgtgctcgc gactaaaagt caggtagcag caggcattaa cccagtgata 1680 gaacatcaaa gtcaaataag tatggtttag cattactgcg tatcttgcga ttgcgtgctg 1740 gtggtcaaca gtgcaaacct agtggcttcg cactcaaatg tagacaagca ttaaccaact 1800 acttctaacg tatgttcagg aagaatggct attctcgtca ggtgaatgcg taatcgaatg 1860 agtagtatgg tcgaacgtcc cgctatagtc tgacacgatt caggcaaagt tggagtgcaa 1920 agccatgatt tggtcgatgc ggcaaatgtt gcttaggact attctatggt cttgcgtaat 1980 tgtgaagatt tgccagttat ctaaggagac gtggtgattc gctctatagc tgtatctaat 2040 ctgccagatt g 2051 <210> 85 <211> 1971 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 85 gttggtgtat ttatccgcta cgtggtggat gaacatggat gcgaacgaca tttcgaaata 60 tacgcaatga tatccaaccc taccgaaaca gcaataattg tagaaggtag catatcatgc 120 cgagagttta ctacgttgta gcgagtttag ttgaaatagt ttgtgatgtg taaactaaaa 180 cataccaaat ggtaaaactt gtcaccccca tttacattgc gacagacaca gttatggagt 240 ggatggaagt gtgttcgact gtcaagaccc ttctataata gtcgaaccag tacggtaagt 300 gggcaacatg cccatataag tcgaagcatt tcaaatggtt catccagtag acgacgatgt 360 cctagacagt catgtctctt ccccatttga agtcatgtag tggttgtcta ctttttcatt 420 gaagacgtcc acctcaacgc tatgaacgtt agttgagaca atgttaaagc aaacgacaac 480 gtcattgtga tctacataca cagtggatgg ttagcgtaaa catggtggaa cgtactttga 540 ctgcgctgca agaaatggtt gggtcgatcg taatgctagt cgttacatcg gaacaagcca 600 aaacaaatca ttcgctggat ttagacctac tgcacgacga cgtcgacaca agacattctt 660 gaaagcgcta gtccatgaaa atgtcctagc tttgaacgtt tatcaaatgc aagttgtggg 720 agttagtcat gtgagagttg tgcaagaaag tgttaacgtt ctagccgtgc tcagtaacaa 780 ttaccattcg gcggtaacaa acgtaagttg tcactgcact catgagcatg aagttagacc 840 agtagttggg tcagaaatta gacgtggttt agtctggtta ccgtggaaag aatagctgaa 900 ctcaaagaag ccgacaaaga taaacaaaga agaaacggtt aatacgattg aggagaaata 960 ttcaatccaa cggcaactat taacgtttca actagaatct acgtttcata cgcggattcc 1020 acatccgttc ttgcaattaa agaaacgatg tagaaaacgc tttggttggc gacaacttgg 1080 gcggcaaaat aattatggta ggtaattgaa gtagcgttaa aacgcaaaaa gaaggaagca 1140 cggatcactg caaaaaggac tactcaataa tggtttctaa agtcattatg ctggtgctcc 1200 ctatggcggt ggtcagtact gtaactaaag atggattacg gcacttgtag agcacgtatc 1260 agacccagct ttgaaagaag gctgagttcg gttcgggttt cacgtaggcc tagaacgagg 1320 tatagaagtg gttaacaaag atttggttac catgtaagaa gcggtagacg ccatggccta 1380 gtaggaagaa tgcttgttca cggagccgac gaagttagac gagtggcaga agaaagagca 1440 ttatcttggt actcggactg aatgcgtact acggtcgtcc aagttgcaag aggtattaga 1500 gtcattcgaa tagctgtggg agtggtattt atagcataat aagtgtaaaa aacattccca 1560 acagcatatg tgtagccaag aagcgttgtg ctcgcgacta aaagtcaggt agcagcaggc 1620 attaacccag tgatagaaca tcaaagtcaa ataagtatgg tttagcatta ctgcgtatct 1680 tgcgattgcg tgctggtggt caacagtgca aacctagtgg cttcgcactc aaatgtagac 1740 aagcattaac caactacttc taacgtatgt tcaggaagaa tggctattct cgtcaggtga 1800 atgcgtaatc gaatgagtag tatggtcgaa cgtcccgcta tagtctgaca cgattcaggc 1860 aaagttggag tgcaaagcca tgatttggtc gatgcggcaa atgttgctta ggactattct 1920 atggtcttgc gtaattgtga agatttgcca gttatctaag gagacgtggt g 1971 <210> 86 <211> 686 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 86 gctacgtggt ggatgaacat ggatgcgaac gacatttcga aatatacgca atgatatcca 60 accctaccga aacagcaata attgtagaag gtagcatatc atgccgagtc atcgacatgc 120 ctttatcgac acacgacgtg attggagttt actacgttgt agcgagttta gttgaaatag 180 tttgtgatgt gtaaactaaa acataccaaa tggtaaaact tgtcaccccc atttacattg 240 cgacagacac agttatggag tggatggaag tgtgttcgac tgtcaagacc cttctataat 300 agtcgaacca gtacggtaag tgggcaacat gcccatataa gtcgaagcat ttcaaatggt 360 tcatccagta gacgacgatg tcctagacag tcatgtctct tccccatttg aagtcatgta 420 gtggttgtct actttttcat tgaagacgtc cacctcaacg ctatgaacgt tagttgagac 480 aatgttaaag caaacgacaa cgtcattgtg atctacatac acagtggatg gttagcgtaa 540 acatggtgga acgtactttg actgcgctgc aagaaatggt tgggtcgatc gtaatgctag 600 tcgttacatc ggaacaagcc aaaacaaatc attcgctgga tttagaccta ctgcacgacg 660 acgtcgacac aagacattct tgaaag 686 <210> 87 <211> 740 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 87 gtaaaacttg tcacccccat ttacattgcg acagacacag ttatggagtg gatggaagtg 60 tgttcgactg tcaagaccct tctataatag tcgaaccagt acggtaagtg ggcaacatgc 120 ccatataagt cgaagcattt caaatggttc atccagtaga cgacgatgtc ctagacagtc 180 atgtctgtca catctatcac gcctcctacg ttgtgccata acgaagaata atagtgataa 240 gagacatcga agctgtcgta gtcgttcgct aaattgctta caatttagtg ctctaagtga 300 acaatgtttc atcttgagaa gcaaacgaag tagaagttga cgacgcattc gaagacgtgg 360 acaatgtgac acgagtatcg aaacatgatc caacgaacat cgaagaagtt actactgaac 420 tacgggtcag cttccccatt tgaagtcatg tagtggttgt ctactttttc attgaagacg 480 tccacctcaa cgctatgaac gttagttgag acaatgttaa agcaaacgac aacgtcattg 540 tgatctacat acacagtgga tggttagcgt aaacatggtg gaacgtactt tgactgcgct 600 gcaagaaatg gttgggtcga tcgtaatgct agtcgttaca tcggaacaag ccaaaacaaa 660 tcattcgctg gatttagacc tactgcacga cgacgtcgac acaagacatt cttgaaaggt 720 aattgacgtg gacgtttcag 740 <210> 88 <211> 523 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 88 gccgcatcga ccaaatcatg gctttgcact ccaactttgc ctgaatcgtg tcagactata 60 gcgggacgtt cgaccatact actcattcga ttacgcattc acctgacgag aatagccatt 120 cttcctgaac atacgttaga agtagttggt taatgcttgt ctacatttga gtgcgaagcc 180 actaggtttg cactgttgac caccagcacg caatcgcaag atacgcagta atgctaaacc 240 atacttattt gactttgatg ttctatcact gggttaatgc ctgctgctac ctgactttta 300 gtcgcgagca caacgcttct tggctacaca tatgctgttg ggaatgtttt ttacacttat 360 tatgctataa ataccactcc cacagctatt cgaatgactc taatacctct tgcaacttgg 420 acgaccgtag tacgcattca gtccgagtac caagataatg ctctttcttc tgccactcgt 480 ctaacttcgt cggctccgtg aacaagcatt cttcctacta ggc 523 <210> 89 <211> 424 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 89 gttgaggtgg acgtcttcaa tgaaaaagta gacaaccact acatgacttc aaatggggaa 60 gctgacccgt agttcagtag taacttcttc gatgttcgtt ggatcatgtt tcgatactcg 120 tgtcacattg tccacgtctt cgaatgcgtc gtcaacttct acttcgtttg cttctcaaga 180 tgaaacattg ttcacttaga gcactaaatt gtaagcaatt tagcgaacga ctacgacagc 240 ttcgatgtct cttatcacta ttattcttcg ttatggcaca acgtaggagg cgtgatagat 300 gtgacagaca tgactgtcta ggacatcgtc gtctactgga tgaaccattt gaaatgcttc 360 gacttatatg ggcatgttgc ccacttaccg tactggttcg actattatag aagggtcttg 420 acag 424 <210> 90 <211> 2467 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 90 gtggagaagc aaatacttgg ataccgaatt tagaggccat aggttatgga aaaagtcagt 60 ggattagata gtaaaagtcg aaatctttat attcggttcg atgtactacg ttattcgcga 120 ctgttctgtc atgatagttt tttatatagt cctaataaaa ggagttttta aagtaaatga 180 catttcagta gtaaaagttt aagagctttt tgttctgcca gacgttctga tagttgagga 240 aaacgtgtgg tcagctaata acttgtggta tggagtaaac gaacatccaa cagtatgtac 300 aatcgtatga gaaaggacaa atctgaagac gatattagag ggagtccaac aagtttgtga 360 tgattcacgt aaggaaatcg aaatgtaaga ttattcgtca atggaggtat aagacaacac 420 accaagtaat tatacgcgac gagttttcat gttgacgatc cctgagacta tatcatacaa 480 aattgaccca aataataaac tcgttccaat agttgaagtg ttgattaaca cattgagaca 540 agttgtccgt actttaatca tcaaagtttt ggactgacct ataaatgaaa tattcgcgtt 600 gtcttcagtt taagaagtga tattattttc ggtttcaagc ctatgtggag agatacagga 660 aataggtaaa ctataactaa tataaatata cgcagacgaa gcctgttaac tttaccaaat 720 ccaaatagcg ggagacacta aacttacaag aaatgtacgg taagacaggg aaacagggtt 780 tgaagtttgg aggtacaaaa tcttaatcct tcacaactga atcaacattt acattcggtt 840 acgttcgggg tttgtagtag taattgttga ggtaatttta attgcactta cagacgtgga 900 tttggtgaaa cattatgtgg tagattcata tttccgaatc ttaatagacg attttatgat 960 ggtgatcgtg gtttcttttc aaggtttggt ggacgaaaat gaaaaaatca caatttaacg 1020 tttcatattc atcacaatcc gaaatcgaga tggagagaaa gtttgactaa actttgttac 1080 agtatttttg aaggcaatca acctggaact ttggatttca tcaagtaaac acccatattt 1140 agcgaaaatg caagctaact gcgaactaac aaggtaactc tttttcgatt tcaaaatatg 1200 gtcagtagca atatgttaag tgatccattt tggaggggtg gggatttgtt caatgaatga 1260 ggcaataaac caccgcaacc tctgtaattg acgagaaact tgtataaggt catcaaaaag 1320 aatagatgaa cataaagatt gaatacggct gggtgaggtt aattcagatt tcccaaatta 1380 tgtagatgaa aagatggtaa gtttattgat aggtaaaaaa tcaagagagg gtaataaaga 1440 agaaagaata atcaaaagat agagcatgca aacattacat ggtagtattg ttataggcgg 1500 tatattggta aatgacgatc cttgacttag ttttttacat cgaacggttg caaatcctag 1560 catacttgat attaatagta gatactgaaa tatatctgag tatatcaagg gaaatcgaga 1620 tgtaacaata agtcgaggaa attaaggtca atttagtgct tatcatagaa ataactactt 1680 aagcccttaa cgaactaact caaattaacg aggtagaggt tattctgaat tgtacgaacg 1740 tagtcgaagt ttgttaccaa gggtaaagcc aaaacaaaac gatttgacaa gagaatctat 1800 tccatgtagt agtgcgtttg tacgacgttc tattcgatgt aaatcattag gacgagggtt 1860 tgaagcaact cggaaagatg gtcggttaga atttaaaagt actgaacgga attaacgaag 1920 ttggctacta cctaatagtc tttgcttagg atttagtagt tcgcaattgg gtcaaagtgg 1980 tcgctttggt cgtcgacgtc aagactcaac aagtcattta ccgctgtacg aaccatctca 2040 tttaacaggt tctcaaccaa atagcggtag gagtagaaag cacttaccat cgtacgaact 2100 ttgacaacga aaggttcaaa ggtaaccaag gcgtagtagt gagttaggtc aatgtataaa 2160 aactcccttt cttattttga gtcgtcaatg tagtcgagaa agtatgcact tagtgccatt 2220 attattagac aagatagtcc gttctttagt cgaagttctt gttgtaataa caaaagtaga 2280 gcagattacg atccgaaatt tcatggacac agatatttga gatcaacatt tgattcgttg 2340 acgtatttat gttgattcgg ttagcgagag ttacaaagtt agtaatagaa agaaagagga 2400 aataataatc aaaagattaa gcatgcaaac attaagagga cacatatgta ttggtggcat 2460 ttgcgtt 2467 <210> 91 <211> 1807 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 91 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggagtaaac gaacatccaa 60 cagtatgtac aatcgtatga gaaaggacaa atctgaagac gatattagag ggagtccaac 120 aagtttgtga tgattcacgt aaggaaatcg aaatgtaaga ttattcgtca atggaggtat 180 aagacaacac accaagtaat tatacgcgac gagttttcat gttgacgatc cctgagacta 240 tatcatacaa aattgaccca aataataaac tcgttccaat agttgaagtg ttgattaaca 300 cattgagaca agttgtccgt actttaatca tcaaagtttt ggactgacct ataaatgaaa 360 tattcgcgtt gtcttcagtt taagaagtga tattattttc ggtttcaagc ctatgtggag 420 agatacagga aataggtaaa ctataactaa tataaatata cgcagacgaa gcctgttaac 480 tttaccaaat ccaaatagcg ggagacacta aacttacaag aaatgtacgg taagacaggg 540 aaacagggtt tgaagtttgg aggtacaaaa tcttaatcct tcacaactga atcaacattt 600 acattcggtt acgttcgggg tttgtagtag taattgttga ggtaatttta attgcactta 660 cagacgtgga tttggtgaaa cattatgtgg tagattcata tttccgaatc ttaatagacg 720 attttatgat ggtgatcgtg gtttcttttc aaggtttggt ggacgaaaat gaaaaaatca 780 caatttaacg tttcatattc atcacaatcc gaaatcgaga tggagagaaa gtttgactaa 840 actttgttac agtatttttg aaggcaatca acctggaact ttggatttca tcaagtaaac 900 acccatattt agcgaaaatg caagctaact gcgaactaac aaggtaactc tttttcgatt 960 tcaaaatatg gtcagtagca atatgttaag tgatccattt tggaggggtg gggatttgtt 1020 caatgaatga ggcaataaac caccgcaacc tctgtaattg acgagaaact tgtataaggt 1080 catcaaaaag aatagatgaa cataaagatt gaatacggct gggtgaggtt aattcagatt 1140 tcccaaatta tgtagatgaa aagatggtaa gtttattgat aggtaaaaaa tcaagagagg 1200 gtaataaaga agaaagaata atcaaaagat agagcatgca aacattacat ggtagtattg 1260 ttataggcgg tatattggta aatgacgatc cttgacttag ttttttacat cgaacggttg 1320 caaatcctag catacttgat attaatagta gatactgaaa tatatctgag tatatcaagg 1380 gaaatcgaga tgtaacaata agtcgaggaa attaaggtca atttagtgct tatcatagaa 1440 ataactactt aagcccttaa cgaactaact caaattaacg aggtagaggt tattctgaat 1500 tgtacgaacg tagtcgaagt ttgttaccaa gggtaaagcc aaaacaaaac gatttgacaa 1560 gagaatctat tccatgtagt agtgcgtttg tacgacgttc tattcgatgt aaatcattag 1620 gacgagggtt tgaagcaact cggaaagatg gtcggttaga atttaaaagt actgaacgga 1680 attaacgaag ttggctacta cctaatagtc tttgcttagg atttagtagt tcgcaattgg 1740 gtcaaagtgg tcgctttggt cgtcgacgtc aagactcaac aagtcattta ccgctgtacg 1800 aaccatc 1807 <210> 92 <211> 2018 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 92 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggattagat agtaaaagtc 60 gaaatcttta tattcggttc gatgtactac gttattcgcg actgttctgt catgatagtt 120 ttttatatag tcctaataaa aggagttttt aaagtaaatg acatttcagt agtaaaagtt 180 taagagcttt ttgttctgcc agacgttctg atagttgagg aaaacgtgtg gtcagctaat 240 aacttgtggt atggagtaaa cgaacatcca acagtatgta caatcgtatg agaaaggaca 300 aatctgaaga cgatattaga gggagtccaa caagtttgtg atgattcacg taaggaaatc 360 gaaatgtaag attattcgtc aatggaggta taagacaaca caccaagtaa ttatacgcga 420 cgagttttca tgttgacgat ccctgagact atatcataca aaattgaccc aaataataaa 480 ctcgttccaa tagttgaagt gttgattaac acattgagac aagttgtccg tactttaatc 540 atcaaagttt tggactgacc tataaatgaa atattcgcgt tgtcttcagt ttaagaagtg 600 atattatttt cggtttcaag cctatgtgga gagatacagg aaataggtaa actataacta 660 atataaatat acgcagacga agcctgttaa ctttaccaaa tccaaatagc gggagacact 720 aaacttacaa gaaatgtacg gtaagacagg gaaacagggt ttgaagtttg gaggtacaaa 780 atcttaatcc ttcacaactg aatcaacatt tacattcggt tacgttcggg gtttgtagta 840 gtaattgttg aggtaatttt aattgcactt acagacgtgg atttggtgaa acattatgtg 900 gtagattcat atttccgaat cttaatagac gattttatga tggtgatcgt ggtttctttt 960 caaggtttgg tggacgaaaa tgaaaaaatc acaatttaac gtttcatatt catcacaatc 1020 cgaaatcgag atggagagaa agtttgacta aactttgtta cagtattttt gaaggcaatc 1080 aacctggaac tttggatttc atcaagtaaa cacccatatt tagcgaaaat gcaagctaac 1140 tgcgaactaa caaggtaact ctttttcgat ttcaaaatat ggtcagtagc aatatgttaa 1200 gtgatccatt ttggaggggt ggggatttgt tcaatgaatg aggcaataaa ccaccgcaac 1260 ctctgtaatt gacgagaaac ttgtataagg tcatcaaaaa gaatagatga acataaagat 1320 tgaatacggc tgggtgaggt taattcagat ttcccaaatt atgtagatga aaagatggta 1380 agtttattga taggtaaaaa atcaagagag ggtaataaag aagaaagaat aatcaaaaga 1440 tagagcatgc aaacattaca tggtagtatt gttataggcg gtatattggt aaatgacgat 1500 ccttgactta gttttttaca tcgaacggtt gcaaatccta gcatacttga tattaatagt 1560 agatactgaa atatatctga gtatatcaag ggaaatcgag atgtaacaat aagtcgagga 1620 aattaaggtc aatttagtgc ttatcataga aataactact taagccctta acgaactaac 1680 tcaaattaac gaggtagagg ttattctgaa ttgtacgaac gtagtcgaag tttgttacca 1740 agggtaaagc caaaacaaaa cgatttgaca agagaatcta ttccatgtag tagtgcgttt 1800 gtacgacgtt ctattcgatg taaatcatta ggacgagggt ttgaagcaac tcggaaagat 1860 ggtcggttag aatttaaaag tactgaacgg aattaacgaa gttggctact acctaatagt 1920 ctttgcttag gatttagtag ttcgcaattg ggtcaaagtg gtcgctttgg tcgtcgacgt 1980 caagactcaa caagtcattt accgctgtac gaaccatc 2018 <210> 93 <211> 1083 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 93 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggtacgaat tacgaacacc 60 ttaacattag ggaaacttag atagttgtac aaagtaacgt ataacaagta tcgcgtatgg 120 attagatagt aaaagtcgaa atctttatat tcggttcgat gtactacgtt attcgcgact 180 gttctgtcat gatagttttt tatatagtcc taataaaagg agtttttaaa gtaaatgaca 240 tttcagtagt aaaagtttaa gagctttttg ttctgccaga cgttctgata gttgaggaaa 300 acgtgtggtc agctaataac ttgtggtatg gagtaaacga acatccaaca gtatgtacaa 360 tcgtatgaga aaggacaaat ctgaagacga tattagaggg agtccaacaa gtttgtgatg 420 attcacgtaa ggaaatcgaa atgtaagatt attcgtcaat ggaggtataa gacaacacac 480 caagtaatta tacgcgacga gttttcatgt tgacgatccc tgagactata tcatacaaaa 540 ttgacccaaa taataaactc gttccaatag ttgaagtgtt gattaacaca ttgagacaag 600 ttgtcctatt gttataggcg gtatattggt aaatgacgat ccttgactta gttttttaca 660 tcgaacggtt gcaaatccta gcatacttga tattaatagt agcatcgtac gaactttgac 720 aacgaaaggt tcaaaggtaa ccaaggcgta gtagtgagtt aggtcaatgt ataaaaactc 780 cctttcttat tttgagtcgt caatgtagtc gagaaagtaa gatgcactta gtgccattat 840 tattagacaa gatagtccgt tctttagtcg aagttcttgt tgtaataaca aaagtagagc 900 agattacgat ccgaaatttc atggacacag atatttgaga tcaacatttg attcgttgac 960 gtatttatgt tgattcggtt agcgagagtt acaaagttag taatagaaag aaagaggaaa 1020 taataatcaa aagattaagc atgcaaacat taagaggaca catatgtatt ggtggcattt 1080 gcg 1083 <210> 94 <211> 436 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 94 gtacgaatta cgaacacctt aacattaggg aaacttagat agttgtacaa agtaacgtat 60 aacaagtatc gcgtatggat tagatagtaa aagtcgaaat ctttatattc ggttcgatgt 120 actacgttat tcgcgactgt tctgtcatga tagtttttta tatagtccta ataaaaggag 180 tttttaaagt aaatgacatt tcagtagtaa aagtttaaga gctttttgtt ctgccagacg 240 ttctgatagt tgaggaaaac gtgtggtcag ctaataactt gtggtatgtt tggaggtaca 300 aaatcttaat ccttcacaac tgaatcaaca tttacattcg gttacgttcg gggtttgtag 360 tagtaattgt tgaggtaatt ttaattgcac ttacagacgt ggatttggtg aaacattatg 420 tggtagattc atattt 436 <210> 95 <211> 2373 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 95 gtggagaagc aaatacttgg ataccgaatt tagaggccat aggttatgga aaaagtcagt 60 ggtacgaatt acgaacacct taacattagg gaaacttaga tagttgtaca aagtaacgta 120 taacaagtat cgcgtatgga gtaaacgaac atccaacagt atgtacaatc gtatgagaaa 180 ggacaaatct gaagacgata ttagagggag tccaacaagt ttgtgatgat tcacgtaagg 240 aaatcgaaat gtaagattat tcgtcaatgg aggtataaga caacacacca agtaattata 300 cgcgacgagt tttcatgttg acgatccctg agactatatc atacaaaatt gacccaaata 360 ataaactcgt tccaatagtt gaagtgttga ttaacacatt gagacaagtt gtccgtactt 420 taatcatcaa agttttggac tgacctataa atgaaatatt cgcgttgtct tcagtttaag 480 aagtgatatt attttcggtt tcaagcctat gtggagagat acaggaaata ggtaaactat 540 aactaatata aatatacgca gacgaagcct gttaacttta ccaaatccaa atagcgggag 600 acactaaact tacaagaaat gtacggtaag acagggaaac agggtttgaa gtttggaggt 660 acaaaatctt aatccttcac aactgaatca acatttacat tcggttacgt tcggggtttg 720 tagtagtaat tgttgaggta attttaattg cacttacaga cgtggatttg gtgaaacatt 780 atgtggtaga ttcatatttc cgaatcttaa tagacgattt tatgatggtg atcgtggttt 840 cttttcaagg tttggtggac gaaaatgaaa aaatcacaat ttaacgtttc atattcatca 900 caatccgaaa tcgagatgga gagaaagttt gactaaactt tgttacagta tttttgaagg 960 caatcaacct ggaactttgg atttcatcaa gtaaacaccc atatttagcg aaaatgcaag 1020 ctaactgcga actaacaagg taactctttt tcgatttcaa aatatggtca gtagcaatat 1080 gttaagtgat ccattttgga ggggtgggga tttgttcaat gaatgaggca ataaaccacc 1140 gcaacctctg taattgacga gaaacttgta taaggtcatc aaaaagaata gatgaacata 1200 aagattgaat acggctgggt gaggttaatt cagatttccc aaattatgta gatgaaaaga 1260 tggtaagttt attgataggt aaaaaatcaa gagagggtaa taaagaagaa agaataatca 1320 aaagatagag catgcaaaca ttacatggta gtattgttat aggcggtata ttggtaaatg 1380 acgatccttg acttagtttt ttacatcgaa cggttgcaaa tcctagcata cttgatatta 1440 atagtagata ctgaaatata tctgagtata tcaagggaaa tcgagatgta acaataagtc 1500 gaggaaatta aggtcaattt agtgcttatc atagaaataa ctacttaagc ccttaacgaa 1560 ctaactcaaa ttaacgaggt agaggttatt ctgaattgta cgaacgtagt cgaagtttgt 1620 taccaagggt aaagccaaaa caaaacgatt tgacaagaga atctattcca tgtagtagtg 1680 cgtttgtacg acgttctatt cgatgtaaat cattaggacg agggtttgaa gcaactcgga 1740 aagatggtcg gttagaattt aaaagtactg aacggaatta acgaagttgg ctactaccta 1800 atagtctttg cttaggattt agtagttcgc aattgggtca aagtggtcgc tttggtcgtc 1860 gacgtcaaga ctcaacaagt catttaccgc tgtacgaacc atctcattta acaggttctc 1920 aaccaaatag cggtaggagt agaaagcact taccatcgta cgaactttga caacgaaagg 1980 ttcaaaggta accaaggcgt agtagtgagt taggtcaatg tataaaaact ccctttctta 2040 ttttgagtcg tcaatgtagt cgagaaagtg tccaagcaaa acgtaacgaa acggttgagt 2100 aaggacttgc acactatgcc cttgcgggtt tacctgcttg acgtagtcct gccacttatt 2160 atccgtgatt gttacctaat ggacaaccta atcgttagct taaagaagat gcacttagtg 2220 ccattattat tagacaagat agtccgttct ttagtcgaag ttcttgttgt aataacaaaa 2280 gtagagcagt attttgacgt atagggagac agtcattagg tcattaaccc agttactaag 2340 gacaacgtaa ccatcgaagt tattgaactt cag 2373 <210> 96 <211> 779 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 96 gataccgaat ttagaggcca taggttatgg aaaaagtcag tggtacgaat tacgaacacc 60 ttaacattag ggaaacttag atagttgtac aaagtaacgt ataacaagta tcgcgtatga 120 tagtaaaagt cgaaatcttt atattcggtt cgatgtacta cgttattcgc gactgttctg 180 tcatgatagt tttttatata gtcctaataa aaggagtttt taaagtaaat gacatttcag 240 tagtaaaagt ttaagagctt tttgttctgc cagacgttct gatagttgag gaaaacgtgt 300 ggtcagctaa taacttgtgg agtaaacgaa catccaacag tatgtacaat cgtatgagaa 360 aggacaaatc tgaagacgat attagaggga gtccaacaag tttgtgatga ttcacgtaag 420 gaaatcgaaa tgtaagatta ttcgtcaatg gaggtataag acaacacacc aagtaattat 480 acgcgacgag ttttcatgtt gacgatccct gagactatat catacaaaat tgacccaaat 540 aataaactcg ttccaatagt tgaagtgttg attaacacat tgagacaagt tgtccgatta 600 cgatccgaaa tttcatggac acagatattt gagatcaaca tttgattcgt tgacgtattt 660 atgttgattc ggttagcgag agttacaaag ttagtaatag aaagaaagag gaaataataa 720 tcaaaagatt aagcatgcaa acattaagag gacacatatg tattggtggc atttgcgtt 779 <210> 97 <211> 479 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 97 ggcctctaaa ttcggtatcc aagtatttgc ttctccacct gccaagcgca cataaattct 60 ttgcgagtgt tgtttggcca cttttggtag ctcctgtttc ttggcaattt tggctgacac 120 gttcagtttc ttttgctcca acttcgtaag cagttagtgt aggcgtgcgc taaggcattg 180 cgctactcgc ttccatcgtt ggttctacca cccatatgcc atggcgtcct gtaagtttgc 240 gcctaattac tcgaagcgct ttcatttcta cgaaacgttt tggaataatg tcaacttggg 300 cattgttgaa actacggctt ccttaatgct cttgacaacc ttgtttagtt gttgttcttc 360 cttcttccag catttaataa ccggcttttg ttttaactcc ttcgcttttt cgactttctc 420 ctttagcacc gcaacgttct ttttaagcta caacgctttc ttgaaatgct tctcgtcat 479 <210> 98 <211> 796 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 98 gtattagggc gcattgcgct ttgccgaacg cgtaattaca gaacttgtgg tttcggtttt 60 gtcgatagcg actggaactg ccgaactttg tttaataatt acacgttaat ctgggacaaa 120 aggcaagctg aagatcgcag taacagttgg ttctgcgaaa gttgaccaac ctatgcgact 180 ttctggtttt gcctgttatg aacaacgttg gcaggtaaag aagcggctga ctgctggtcg 240 aagagccaaa tggtcaacat tagcatagct tgtttcgtaa tcgattggct caactgccgc 300 cgtgtccacg cgttaaacgc ggttagtaac atccactgaa ctttcttcgc caacagtaca 360 agctggcagt tgcatgtctt tagtacagga gattgcaacg ctacctacgc aagctttgac 420 tgcgatgcta caccgcacga tagctcgcgt acctacactt ttacgcgcta ctactccgcg 480 aacataagcc acttcatgtt gaacgaatgg agaacgttat ggttcacttc tttatgcgac 540 cgaagaaggc gcagtgtttg cttgacaatt tactgtcgtg tcttcggtaa cagatatgct 600 accaagcgac ctaaccactt taagcagctt caccgtctta cgagacgtta acctaacgca 660 aatgccacca atacatatgt gtcctcttaa tgtttgcatg cttaatcttt tgattattat 720 ttcctctttc tttctattac taactttgta actctcgcta accgaatcaa cataaatacg 780 tcaacgaatc aaatgt 796 <210> 99 <211> 589 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 99 gcgtaattac agaacttgtg gtttcggttt tgtcgatagc gactggaact gccgaacttt 60 gtttaataat tacacgttaa tctgggacaa aaggcaagct gaagatcgca gtaacagttg 120 gttctgcgaa agttgaccaa cctatgcgac tttctggttt tgcctgttat gaacaacgtt 180 ggcaggtaaa gaagcggctg actgctggtc gaagagccaa atggtcaaca ttagcatagc 240 ttgtttcctc tttttcgttg cggagttaag taggagaacg ttatggttca cttctttatg 300 cgaccgaaga aggcgcagtg tttgcttgac aatttactgt cgtgtcttcg gtaacagata 360 tgctaccaag cgacctaacc accttcaccg tcttacgaga cgttaaccta acgcaaatgc 420 caccaataca tatgtgtcct cttaatgttt gcatgcttaa tcttttgatt attatttcct 480 ctttctttct attactaact ttgtaactct cgctaaccga atcaacataa atacgtcaac 540 gaatcaaatg ttgatctcaa atatctgtgt ccatgaaatt tcggatcgt 589 <210> 100 <211> 161 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 100 gctgaccaca cgttttcctc aactatcaga acgtctggca gaacaaaaag ctcttaaact 60 tttactactg aaatgtcatt tactttaaaa actcctttta ttaggactat ataaaaaact 120 atcatgacag aacagtcgcg aataacgtag tacatcgaac c 161 <210> 101 <211> 2253 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 101 gttgaactta tacagaaata ctaaaagtcg aacaaccatt cgatttagga atacagaacg 60 ataacgcagc agaagtagtt gaccaccgtg taacttgaat ggtgatggtc ctggttctat 120 taatacaaat ccttttactt atgctccgtg atacagaaca actaaaagaa ctaaaagaga 180 agaacgacgt gcacgtgcta attatgcgaa ttaacatcgg gttagaaaaa gtaaactaaa 240 ggaaaatggg taaggcatat gccttactaa attgtgtaaa agaagttcta ataagaaccg 300 gaatgtatgt aattattcgc aagggttcca taaggcacat atacaaattt agaaacaacc 360 cgttacatta acaaaggtga acggtgtttc acagatatta gaccattagg taaccaatga 420 catttcacta tcgaaaaggg tatagtatta actactacaa ctaatccgac tcgtcgaggt 480 gatttataca ggactgcagg tagcaaacga cgaggtggac gagggtaagg tggccgacga 540 aagggaggac atgcacctag ttgaacacga ctaggaggac gatggccact acggagtgga 600 acacaaaggt aaagttgcaa taataaccag tacaacaaaa gcatacgtgg aaggtgacaa 660 gcaaacgatg tcttgaacaa caccgtcttc attgtttacc atttgctgtt ggatttgata 720 tttagagcgt atgttgaaga agttggaact ctggacacat tagagactta ttggatcgat 780 caagaggaat gcaatacgaa gtagtttcac tttcgtaggt taccctataa cacgcaaata 840 gatgtaatca acctcttatt aatcatacac tagacatcag atgttaatga cctaacgatt 900 atagtgctga acaacgtcta cgacaatata aacctttgca aagactggtc aaacatcaat 960 gttaaacacg tccagataaa agcaaaaagg taagacgagg attacggaat aattaatata 1020 gtgcttgttc caatcggccc ggcttcagtc attcccggtg gcgaagccat aacatttaaa 1080 aatagcctgt taacttcaag ctgtagtagc ctaaacaggt ttcctaatag tatgcaaaca 1140 acatagttca atagaagtta ttaataaaca tcaaattgtc tttgaacaac acaaacacgt 1200 aagctttcgg caactaaatt aactaaaaaa taactataat tacacagaac acgaagtcat 1260 ctgtttccta ttaaaaaata ggtacttgac aggttacaat ataacctgtt tctagcatgt 1320 ataacaaaat taagcgacga ccgagcgacg acaacaagca gcagccaggt gggcaaaagg 1380 ctaagactgc tttgatgtcc tgagccaact tgccgatacg cagcaacatt ttagaaacgt 1440 catcctgacc gccgccgcca ttgccataaa tgatagcttg gcggtagccg cccaaaacta 1500 tgaaaaaaat taaattaagg aaagacataa acaaggtagt taagccatgc acctagaaaa 1560 ttttgaacgg ctcagctgca acatattagc gctagaaatg acctaccata atgatataga 1620 agataattac cattactgcc accgcctcct ccgctgccgc ctcctccata gcagcttcta 1680 ttcaaacaaa ctccgccgcc gccaccgccg ccataaccac caccataacc accgccgtat 1740 acacaaacgc cgctccttct aagtcttagc tattaataac aaccgtggaa acaaaaaacg 1800 taatataggc tacagctgtg tcaacagcca aatccataac aacaaaatcc atggtgactg 1860 taacagacac tgttacaacc atattattaa ctagatagtg gttacagata atcatgcagc 1920 aacaacatat aaagaagacg gttaaagtta ttgacttatg ttagcatgtg caaattaaca 1980 taggctagtc ttaacctgac tcgctgtcgc gactggcatg caaagtttga cacgccgcgg 2040 ctctagtacg cgtcatcttt cagctgtaat aactacaaac acatcaaaaa aagtcggttt 2100 aaaacaactt gtgaaacgtc aacttggtaa ataaacctat tattaaagaa tctagatcaa 2160 tgtccaaaaa gattatttcc attgttacgt ctaaatacag attctgcgaa aacaagccat 2220 tagtatagta gaagaactaa ccatcgtatg ttc 2253 <210> 102 <211> 2059 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 102 gtgtaaccat cttacggatg taatctaacg taaacaccag agaaacaacc atcaaaacgg 60 cataatgggc aagcattatt aggaacatct aaatataaaa ccattgcctt aactcacata 120 acgctacact tggtaacacc accatatgca gtgcaagaac gttgggtaag agaacatcga 180 acgtatggtc tgtttacata agctcaaaaa taattttaca gggtaaaacg tcaatcatta 240 cttaacagca gtaatgctta caaatgtttc ggttagtatt taaaaccgta acgaacgcca 300 cgtgtaaaaa caagaggttt tattaatatt gggtatatta gatgtatccg tcgattaacc 360 caccataacc aaactaaatt aaagcaacga cttatgcgaa atcctccttg tttacaaact 420 aacagtaatg caagtcgtaa taaacgtctt aaatatttta taggtaaaag atttggacca 480 tatcctcagt actgtaatga taaacaccga cgaagtctac atcgttgcaa cggaaaattc 540 catgaaagtc gtatgtttta tggtaaactt ccgaatttag caaaaagggt tgtaaaaggt 600 atacgtcatc atggtaagaa atacagctgt attagtatat aaggttgtag aaaaccacat 660 aataatatgt ctttagcact agacatcaga tgttaatgac ctaacgatta tagtgctgaa 720 caacgtctac gacaatataa acctttgcaa agactggtca aacatcaatg ttaaacacgt 780 ccagataaaa gcaaaaaggt aagacgagga ttacggaata attaatatag tgcttgttcc 840 aatcggcccg gcttcagtca ttcccggtgg cgaagccata acatttaaaa atagcctgtt 900 aacttcaagc tgtagtagcc taaacaggtt tcctaatagt atgcaaacaa catagttcaa 960 tagaagttat taataaacat caaattgtct ttgaacaaca caaacacgta agctttcggc 1020 aactaaatta actaaaaaat aactataatt acacagaaca cgaagtcatc tgtttcctat 1080 taaaaaatag gtacttgaca ggttacaata taacctgttt ctagcatgta taacaaaatt 1140 aagcgacgac cgagcgacga caacaagcag cagccaggtg ggcaaaaggc taagactgct 1200 ttgatgtcct gagccaactt gccgatacgc agcaacattt tagaaacgtc atcctgaccg 1260 ccgccgccat tgccataaat gatagcttgg cggtagccgc ccaaaactat gaaaaaaatt 1320 aaattaagga aagacataaa caaggtagtt aagccatgca cctagaaaat tttgaacggc 1380 tcagctgcaa catattagcg ctagaaatga cctaccataa tgatatagaa gataattacc 1440 attactgcca ccgcctcctc cgctgccgcc tcctccatag cagcttctat tcaaacaaac 1500 tccgccgccg ccaccgccgc cataaccacc accataacca ccgccgtata cacaaacgcc 1560 gctccttcta agtcttagct attaataaca accgtggaaa caaaaaacgt aatataggct 1620 acagctgtgt caacagccaa atccataaca acaaaatcca tggtgactgt aacagacact 1680 gttacaacca tattattaac tagatagtgg ttacagataa tcatgcagca acaacatata 1740 aagaagacgg ttaaagttat tgacttatgt tagcatgtgc aaattaacat aggctagtct 1800 taacctgact cgctgtcgcg actggcatgc aaagtttgac acgccgcggc tctagtacgc 1860 gtcatctttc agctgtaata actacaaaca catcaaaaaa agtcggttta aaacaacttg 1920 tgaaacgtca acttggtaaa taaacctatt attaaagaat ctagatcaat gtccaaaaag 1980 attatttcca ttgttacgtc taaatacaga ttctgcgaaa acaagccatt agtatagtag 2040 aagaactaac catcgtatg 2059 <210> 103 <211> 670 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 103 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggagtgcaga tacagaacaa 120 ctaaaagaac taaaagagaa gaacgacgtg cacgtgctaa ttatgcgaat taacatcggg 180 ttagaaaaag taaactaaag gaaaatgggt aaggcatatg ccttactaaa ttgtgtaaaa 240 gaagttctaa taagaaccgg aatgtatgta attattcgca agggttccat aaggcacata 300 tacaaattta gaaacaaccc gttacattaa caaaggtgaa cggtgtttca cagatattag 360 accattaggt aaccaatgac atttcactat cgaaaagggt atagtattaa ctactacaac 420 taatccgact cgtcgaggtg atttatacag gactgcaggt agcaaacgac gaggtggacg 480 agggtaaggt ggccgacgaa agggaggaca tgcacctagt tgaacacgac taggaggacg 540 atggccacta cggagtggaa cacaaaggta aagttgcaat aataaccagt acaacaaaag 600 catacgtgga aggtgacaag caaacgatgt cttgaacaac accgtcttca ttgtttacca 660 tttgctgttg 670 <210> 104 <211> 592 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 104 gcaattagga tcaggtggtt aatctgatac gatacagaac aactaaaaga actaaaagag 60 aagaacgacg tgcacgtgct aattatgcga attaacatcg ggttagaaaa agtaaactaa 120 aggaaaatgg gtaaggcata tgccttacta aattgtgtaa aagaagttct aataagaacc 180 ggaatgtatg taattattcg caagggttcc ataaggcaca tatacaaatt tagaaacaac 240 ccgttacatt aacaaaggtg aacggtgttt cacagatatt agaccattag gtaaccaatg 300 acatttcact atcgaaaagg gtatagtatt aactactaca actaatccga ctcgtcgagg 360 tgatttatac aggactgcag gtagcaaacg acgaggtgga cgagggtaag gtggccgacg 420 aaagggagga catgcaccta gttgaacacg actaggagga cgatggccac tacggagtgg 480 aacacaaagg taaagttgca ataataacca gtacaacaaa agcatacgtg gaaggtgaca 540 agcaaacgat gtcttgaaca acaccgtctt cattgtttac catttgctgt tg 592 <210> 105 <211> 626 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 105 gccaaggtga accaggacga agtcgatgtt tcagaacctc ctttaggcct tttagacggt 60 ttaaacgaag cactagacca agtaataaaa gcggacgttg cacaggtacg tgcagataca 120 gaacaactaa aagaactaaa agagaagaac gacgtgcacg tgctaattat gcgaattaac 180 atcgggttag aaaaagtaaa ctaaaggaaa atgggtaagg catatgcctt actaaattgt 240 gtaaaagaag ttctaataag aaccggaatg tatgtaatta ttcgcaaggg ttccataagg 300 cacatataca aatttagaaa caacccgtta cattaacaaa ggtgaacggt gtttcacaga 360 tattagacca ttaggtaacc aatgacattt cactatcgaa aagggtatag tattaactac 420 tacaactaat ccgactcgtc gaggtgattt atacaggact gcaggtagca aacgacgagg 480 tggacgaggg taaggtggcc gacgaaaggg aggacatgca cctagttgaa cacgactagg 540 aggacgatgg ccactacgga gtggaacaca aaggtaaagt tgcaataata accagtacaa 600 caaaagcata cgtggaaggt gacaag 626 <210> 106 <211> 617 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 106 gacatcagat gttaatgacc taacgattat agtgctgaac aacgtctacg acaatataaa 60 cctttgcaaa gactggtcaa acatcaatgt taaacacgtc cagataaaag caaaaaggta 120 agacgaggat tacggaataa ttaatatagt gcttgttcca atcggcccgg cttcagtcat 180 tcccggtggc gaagccatgt caccaataaa aactatcggc ggcaatggaa gtggtttttc 240 ggaaactgtt acgggtgcaa gcggccccag tgcagtttgt gccttgccaa tcggcccagc 300 tttcaaacgc aaagttggac gacttattgg cccattctga caacgtcctg ttaaactgcc 360 cggacgttca aacggcggta tcggacgact aacgcgttac ggcggacaac tcgcccccag 420 tcaacaactt ggacacggta aatttgaacg ctaagtcaac tgtagcagtt aaacattgag 480 tattaaaata gatttaagct agcgttaaga acattaaaaa ctaaccagcc aaaccaagga 540 ttacggctgt ggtgtaatcg attgcgaaat agcatgacaa aaaacttaca gtttagaagg 600 tggcggtatt acttaag 617 <210> 107 <211> 2105 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 107 gcagatacag aacaactaaa agaactaaaa gagaagaacg acgtgcacgt gctaattatg 60 cgaattaaca tcgggttaga aaaagtaaac taaaggaaaa tgggtaaggc atatgcctta 120 ctaaattgtg taaaagaagt tctaataaga accggaatgt atgtaattat tcgcaagggt 180 tccataaggc acatatacaa atttagaaac aacccgttac attaacaaag gtgaacggtg 240 tttcacagat attagaccat taggtaacca atgacatttc actatcgaaa agggtatagt 300 attaactact acaactaatc cgactcgtcg aggtgattta tacaggactg caggtagcaa 360 acgacgaggt ggacgagggt aaggtggccg acgaaaggga ggacatgcac ctagttgaac 420 acgactagga ggacgatggc cactacggag tggaacacaa aggtaaagtt gcaataataa 480 ccagtacaac aaaagcatac gtggaaggtg acaagcaaac gatgtcttga acaacaccgt 540 cttcattgtt taccatttgc tgttggattt gatatttaga gcgtatgttg aagaagttgg 600 aactctggac acattagaga cttattggat cgatcaagag gaatgcaata cgaagtagtt 660 tcactttcgt aggttaccct ataacacgca aatagatgta atcaacctct tattaatcat 720 acactagaca tcagatgtta atgacctaac gattatagtg ctgaacaacg tctacgacaa 780 tataaacctt tgcaaagact ggtcaaacat caatgttaaa cacgtccaga taaaagcaaa 840 aaggtaagac gaggattacg gaataattaa tatagtgctt gttccaatcg gcccggcttc 900 agtcattccc ggtggcgaag ccataacatt taaaaatagc ctgttaactt caagctgtag 960 tagcctaaac aggtttccta atagtatgca aacaacatag ttcaatagaa gttattaata 1020 aacatcaaat tgtctttgaa caacacaaac acgtaagctt tcggcaacta aattaactaa 1080 aaaataacta taattacaca gaacacgaag tcatctgttt cctattaaaa aataggtact 1140 tgacaggtta caatataacc tgtttctagc atgtataaca aaattaagcg acgaccgagc 1200 gacgacaaca agcagcagcc aggtgggcaa aaggctaaga ctgctttgat gtcctgagcc 1260 aacttgccga tacgcagcaa cattttagaa acgtcatcct gaccgccgcc gccattgcca 1320 taaatgatag cttggcggta gccgcccaaa actatgaaaa aaattaaatt aaggaaagac 1380 ataaacaagg tagttaagcc atgcacctag aaaattttga acggctcagc tgcaacatat 1440 tagcgctaga aatgacctac cataatgata tagaagataa ttaccattac tgccaccgcc 1500 tcctccgctg ccgcctcctc catagcagct tctattcaaa caaactccgc cgccgccacc 1560 gccgccataa ccaccaccat aaccaccgcc gtatacacaa acgccgctcc ttctaagtct 1620 tagctattaa taacaaccgt ggaaacaaaa aacgtaatat aggctacagc tgtgtcaaca 1680 gccaaatcca taacaacaaa atccatggtg actgtaacag acactgttac aaccatatta 1740 ttaactagat agtggttaca gataatcatg cagcaacaac atataaagaa gacggttaaa 1800 gttattgact tatgttagca tgtgcaaatt aacataggct agtcttaacc tgactcgctg 1860 tcgcgactgg catgcaaagt ttgacacgcc gcggctctag tacgcgtcat ctttcagctg 1920 taataactac aaacacatca aaaaaagtcg gtttaaaaca acttgtgaaa cgtcaacttg 1980 gtaaataaac ctattattaa agaatctaga tcaatgtcca aaaagattat ttccattgtt 2040 acgtctaaat acagattctg cgaaaacaag ccattagtat agtagaagaa ctaaccatcg 2100 tatgt 2105 <210> 108 <211> 570 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 108 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggagtgcaga tacagaacaa 120 ctaaaagaac taaaagagaa gaacgacgtg cacgtgctaa ttatgcgaat taacatcggg 180 ttagaaaaag taaactaaag gaaaatgggt aaggcatatg ccttactaaa ttgtgtaaaa 240 gaagttctaa taagaaccgg aatgtatgta attattcgca agggttccat aaggcacata 300 tacaaattta gaaacaaccc gttacattaa caaaggtgaa cggtgtttca cagatattag 360 accattaggt aaccaatgac atttcactat cgaaaagggt ataggaggac atgcacctag 420 ttgaacacga ctaggaggac gatggccact acggagtgga acacaaaggt aaagttgcaa 480 taataaccag tacaacaaaa gcatacgtgg aaggtgacaa gcaaacgatg tcttgaacaa 540 caccgtcttc attgtttacc atttgctgtt 570 <210> 109 <211> 1567 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 109 gtgtagcata gcaactgttc tgaatgtgat attcggtcag gccacgttga cacttaccaa 60 agcaagacag aaggaagttg acgggacaat agggaagatt ggcaaaggga tttaaacata 120 gtccagttcg cgtggtgaat gacagattgc tactgttacc attggcgata ctacgttggc 180 gactttactt gcctcggtag cgacagaaca agtgtcgtct acgcaacgaa caaccagcgt 240 gttagctgca atgtcccctg tagaagaccc tcttctttct cttctgaatg atgtagctgt 300 ggatagagcg tctcccacgt tagggtctag caaccctccg gtggtgtgtg gtttcggttc 360 gcttggttgg gtgcccaatt tccagtaccg aagaggtaat tgcaacttca attgcttagt 420 tgacgtgacc tttcgggtgt catggactac cgacggtatc tcatacgtga cctcgttgtt 480 gacctcgttc ttcatctgca cagactacaa cgttaggact acggaaccag tgtgaagttg 540 acggacgcgc tagaagtctg acactgacca tgcttgtggc aatcgttgtt catgtagcca 600 ctttgactcg acgttcacga acatacgatg gttagttctt ctactatact aatggcttgt 660 tttcgcagaa tctgtattta gacgtaacaa tggaaataat ctttttggac attgatctag 720 attctttaat aataggttta tttaccaagt tgacgtttca caagttgttt taaaccgact 780 ttttttgatg tgtttgtagt tattacagct gaaagatgac gcgtactaga gccgcggcgt 840 gtcaaacttt gcatgccagt cgcgacagcg agtcaggtta agactagcct atgttaattt 900 gcacatgcta acataagtca ataactttaa ccgtcttctt tatatgttgt tgctgcatga 960 ttatctgtaa ccactatcta gttaataata tggttgtaac agtgtctgtt acagtcacca 1020 tggattttgt tgttatggat ttggctgttg acacagctgt agcctatatt acgttttttg 1080 tttccacggt tgttattaat agctaagact tagaaggagc ggcgtttgtg tatacggcgg 1140 tggttatggt ggtggttatg gcggcggtgg cggcggcgga gtttgtttga atagaagctg 1200 ctatggagga ggcggcagcg gaggaggcgg tggcagtaat ggtaattatc ttctatatca 1260 ttatggtagg tcatttctag cgctaatatg ttgcagctga gccgttcaaa attttctagg 1320 tgcatggctt aactaccttg tttatgtctt tccttaattt aatttttttc atagttttgg 1380 gcggctaccg ccaagctatc atttatggca atggcggcgg cggtcaggat gacgtttcta 1440 aaatgttgct gcgtatcggc aagttggctc aggacatcaa agcagtctta gccttttgcc 1500 cacctggctg ctgcttgttg tcgtcgctcg gtcgtcgctt aattttgtta tacatgctag 1560 aaacagg 1567 <210> 110 <211> 950 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 110 gatcgtggcc cagtcagtgg attacgttga ccatgagtct ggctgttctt ggttcaccat 60 tagctgtggt gacaataacg ggcattgtgg caacgatacg tcgtactgca agtcccactg 120 taactaagac aattcggatt cgaccgatac ttggtctggt tcgttaactt cgcgtacctt 180 ctgctttacc agcaggtcgt caacgacgag ccaccgtaac gagtgtggtt tcggttcgct 240 tggttgggtg cccaatttcc agtaccgaag aggtaattgc aacttcaatt gcttagttga 300 cgtgaccttt cgggtgtcat ggactaccga cggtatctca tacgtgacct cgttgttgac 360 ctcgttcttc atctgcacag actacaacgt taggactacg gaaccagtgt gaagttgacg 420 gacgcgctag tttacagatg cggtttattg gattggttac aacggcaaag gcggagacca 480 ctccaactgt cggaagagta cctcttcaaa ttgccattcc aattgctcgt catggacttt 540 ccactcttag acgacccgat gaagctagaa gtctgacact gaccatgctt gtggcaatcg 600 ttgttcatgt agccactttg actcgacgtt cacgaacata cgatggttag ttcttctact 660 atactaatgg cttgttttcg cagaatctgt atttagacgt aacaatggaa ataatctttt 720 tggacattga tctagattct ttaataatag gtttatttac caagttgacg tttcacaagt 780 tgttttaaac cgactttttt tgatgtgttt gtagttatta cagctgaaag atgacgcgta 840 ctagagccgc ggcgtgtcaa actttgcatg ccagtcgcga cagcgagtca ggttaagact 900 agcctatgtt aatttgcaca tgctaacata agtcaataac tttaaccgtc 950 <210> 111 <211> 1890 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 111 gtagaccttt ccttgccgcg tggaacaaac cgccatgagt gttttacgtt tacggtcggc 60 gaccggagtc acagcgcgac caacggggcc aggcaaaggc ccataggctt ttccatcggc 120 acgcgaccga ttccatcaag tgttaggtgc aatccccatt cgggacggac acgcgagtaa 180 aggtctaaca tgtttcaact ttccagttag tcgccgtcct atattggcaa gaccggatgg 240 ccgtcaacca gttccattct gactatttgc cacattgatt aaagaagtag gagcgttagt 300 cggcgcgtgc gcgggactat ttagcgaagg tcgccgatcg tttgcccgta aaactaactg 360 gcaaaagcct aagtgctgtt tcgggtgcaa tggtataact tcccacattt agccgaatag 420 cgccaacagc gttattattt ggaaccaagc agtgggcgat gtcagcgaca attccacttt 480 ggtagaggtc gttcttttgt agaaaacaat ctaacataac tatgacactt tcggtaaatt 540 caccaccttt accagggtcg taagtggtca agccacgccg accacttcat aagcattggt 600 ggaaatcgca tcgacacaat aaaagtacac caaccagcag aaaacaggta catatacagt 660 ttaacgacct ggatctaaga tgactacagt agcatagcca tagaacatgg cactgaaata 720 tcgatgggcg taaaagtgaa caacgcagac gatggattac gacgccaagc cactataaat 780 gctaattgag tcgaagaagg tactcctttt tggaacacag atattgcaga agcgggaatg 840 aggttgatat tacatcaagt atcaacagac gacctcagaa gggtggtctt ttaaaatgac 900 aacgtacatt aagatgcaga caaacactac tatgctggat gtgaaaatgt catatgtgcg 960 gcaaaaatag acgacattta agaacctagc attataggat ggagtgttgt attaattcac 1020 tctagagtaa tggatcacgg tgccagaaac taaggtggtg taggactaga tgcaagtaac 1080 aacggtcgta ctaaaatcag ctacgcgatc gccgacttag aaggctgaga tggagaaact 1140 tgtctactgg cagacctcac tatcttttag gaaggtcacg aagcggctgg agtgatggct 1200 agtctcagcc actgtcaacg ggaagcgcga cgtttatggt caatataaac tagcagttta 1260 atccgtatgg cgatcttagc ggatgttaca ggatggagaa gatgacaaag tagaagctag 1320 acaactatat caaatcgttg tgtggtctta aaccgacaca gtagcattca tcgttggatg 1380 tggaaggtca cagcctcgtt ggaggagata ctacggtccc gcttttcgtg ggtgacaaag 1440 gtcgcaaagg tttccttagg gaggtcgttt cccggatctt tatctacatc tcctgggtga 1500 aggggatgat ctttctctac atagattgca gagagccagg tcaaatacat tgagaaatct 1560 gtatatgtac tttggtaacg ctctaaggta ggtacacgtt agtgatataa aaagaaggcg 1620 acgacgaact taaattgtta gagcagttta tgggtggtag gttacatcta tttacttcaa 1680 aacgcggtgc cctagcccac aagcattgct ttgtaaaatt gttgaaggta acttgtaaaa 1740 gccgaacatg aacgatggat agtagaccgg gaagaccaac gttttttcac tactccgaaa 1800 gaacacactg aagggaacgg tgtaggttcg gtgagtccgg gactaggtgt ccatgtcgga 1860 gacctcagtg gtgacgtcct cgtagtatag 1890 <210> 112 <211> 1984 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 112 gacttgtagg cccatgaatt acccgcttag gcaatgggta gacctttcct tgccgcgtgg 60 aacaaaccgc catgagtgtt ttacgtttac ggtcggcgac cggagtcaca gcgcgaccaa 120 cggggccagg caaaggccca taggcttttc catcggcacg cgaccgattc catcaagtgt 180 taggtgcaat ccccattcgg gacggacacg cgagagcgtt agtcggcgcg tgcgcgggac 240 tatttagcga aggtcgccgt atcgcgggtg gcggcgtttc cgccataaca acaagcgttt 300 aaggtcagag tccgcgacga atgaaacgcc gtgcaagcgg ctttggtacg gccagtttag 360 ccaaagcctg atccgttcgc cttgggttat catgtaccag tcaagcggtc atcgcgggcg 420 gcgttatcat ggatcgtttg cccgtaaaac taactggcaa aagcctaagt gctgtttcgg 480 gtgcaatggt ataacttccc acatttagcc gaatagcgcc aacagcgtta ttatttggaa 540 ccaagcagtg ggcgatgtca gcgacaattc cactttggta gaggtcgttc ttttgtagaa 600 aacaatctaa cataactatg acactttcgg taaattcacc acctttacca gggtcgtaag 660 tggtcaagcc acgccgacca cttcataagc attggtggaa atcgcatcga cacaataaaa 720 gtacaccaac cagcagaaaa caggtacata tacagtttaa cgacctggat ctaagatgac 780 tacagtagca tagccataga acatggcact gaaatatcga tgggcgtaaa agtgaacaac 840 gcagacgatg gattacgacg ccaagccact ataaatgcta attgagtcga agaaggtact 900 cctttttgga acacagatat tgcagaagcg ggaatgaggt tgatattaca tcaagtatca 960 acagacgacc tcagaagggt ggtcttttaa aatgacaacg tacattaaga tgcagacaaa 1020 cactactatg ctggatgtga aaatgtcata tgtgcggcaa aaatagacga catttaagaa 1080 cctagcatta taggatggag tgttgtatta attcactcta gagtaatgga tcacggtgcc 1140 agaaactaag gtggtgtagg actagatgca agtaacaacg gtcgtactaa aatcagctac 1200 gcgatcgccg acttagaagg ctgagatgga gaaacttgtc tactggcaga cctcactatc 1260 ttttaggaag gtcacgaagc ggctggagtg atggctagtc tcagccactg tcaacgggaa 1320 gcgcgacgtt tatggtcaat ataaactagc agtttaatcc gtatggcgat cttagcggat 1380 gttacaggat ggagaagatg acaaagtaga agctagacaa ctatatcaaa tcgttgtgtg 1440 gtcttaaacc gacacagtag cattcatcgt tggatgtgga aggtcacagc ctcgttggag 1500 gagatactac ggtcccgctt ttcgtgggtg acaaaggtcg caaaggtttc cttagggagg 1560 tcgtttcccg gatctttatc tacatctcct gggtgaaggg gatgatcttt ctctacatag 1620 attgcagaga gccaggtcaa atacattgag aaatctgtat atgtactttg gtaacgctct 1680 aaggtaggta cacgttagtg atataaaaag aaggcgacga cgaacttaaa ttgttagagc 1740 agtttatggg tggtaggtta catctattta cttcaaaacg cggtgcccta gcccacaagc 1800 attgctttgt aaaattgttg aaggtaactt gtaaaagccg aacatgaacg atggatagta 1860 gaccgggaag accaacgttt tttcactact ccgaaagaac acactgaagg gaacggtgta 1920 ggttcggtga gtccgggact aggtgtccat gtcggagacc tcagtggtga cgtcctcgta 1980 gtat 1984 <210> 113 <211> 526 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 113 gtcacctaaa cgtgcgaagt gtgcgaaatt accaccgtaa attcgtccta ctgaaagtgc 60 ttacgcatct tgagagagat tgcagagagc caggtcaaat acattgagaa atctgtatat 120 gtactttggt aacgctctaa ggtaggtaca cgttagtgat ataaaaagaa ggcgacgacg 180 gtagcagagg tctagaaacc gcatgaacga ccaacttatt tgaagagcta gtgcggtgga 240 attgggtcgg aagaacttaa attgttagag cagtttatgg gtggtaggtt acatctattt 300 acttcaaaac gcggtgccct agcccacaag cattgctttg taaaattgtt gaaggtaact 360 tgtaaaagcc gaacatgaac gatggatagt agaccgggaa gaccaacgtt ttttcactac 420 tccgaaagaa cacactgaag ggaacggtgt aggttcggtg agtccgggac taggtgtcca 480 tgtcggagac ctcagtggtg acgtcctcgt agtataggtc cacgct 526 <210> 114 <211> 2473 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 114 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 60 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 120 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 180 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 240 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 300 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 360 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 420 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 480 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 540 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 600 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 660 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 720 taaagcaagg tgcgcaatag taaccttggt aacttcggaa ctgcgtcaag ccgctaactc 780 catcggtatg gaagtcgcta aagtcggtca aacggaagcg gctatcgtcg caactagtcg 840 aatagttgtc gaagaggcca gagcaaccat ggttgccatt gtcttcgtgg tctatgcaag 900 acgtccttgc gtcatggcct aagcatcttc ttcactggtt gctatagcgg aagtcgttac 960 cgaactggtc gccagtcgaa tcgtttgccc catggtagtc atcaccacca tcgcggaaac 1020 caacggtccg aactcacggt aggtagtcca cgtatgtagc ccacctttcg aaggaatcgt 1080 ggtcgccggt ctcctagaaa cggtatatgt cttgcgtcac gcttatggac cggtcatcct 1140 ggccagtagc cgacttgtgg tcgctatagc tttcgctagt ccctgcctta acgtgcagca 1200 tggtatggct attgtcgtcc cggctacaac cactatcgtg gtcttgaaac tggtcgtcgc 1260 tacaaccatc ctagcattgg caccagcggt cgttgaagcc gaaggtagtc tctaactggt 1320 tttcgaagct agtcaaacag tagcgccata tggcctagcc tgaaccttag aaaaaggagt 1380 cgaaggtcct aaacccgctg gtcgcgaacg aacaaaaacg gtcgttaaag gccgtccgga 1440 agcggaaggt cgtcgcccca ggtgaacaag tcctaaagaa acaaaaagtc tctgtagtct 1500 atttaaaacc caacgcaaac gcgaacgcag aatcgtctga acccacgtcc atcattacgg 1560 attatataag gtttctgttt cgttaactag gtagttattg atgtcatccg cactacaagc 1620 taacaggagg taacagatag agcatgagtg gtagtaggga tgaaacagga agtactaatt 1680 acatacaggg actcctggtt gggtgacccc gggacacgta cgtgtcctaa taaacaggag 1740 gcgggtggtt tcctatctac tacggacagg gattttcccc gagtagcgaa ggatatgaaa 1800 cttctagtta tagacatata agacgtagtc caggaagtcg gctgagtcca tgaggtcagt 1860 tttggaggag aagtagttgt cacgatcctt agttaagata acaaagtccg agtagttatc 1920 acgacttgct gactgcgtcc actatgtttc cggggtgggc atggggactt gcacacggat 1980 aactccatcc ggattattaa gacgaggtgg ggtgtgctgg gctcccagga acttcatcgg 2040 agtgacgtag aaccttcagt ttgagtccac gtaggacacc agcgctattg gtgccgacga 2100 cgagtacaaa catccggaaa cttagcatat taggtaagag tttctgtatg ctcggacgtc 2160 gtcgttagag cagacccaga aggtgtccat ggacacacat catacaagac aacttggtca 2220 acttttacgg ggctggggcc aattatcccg ggagtcctca cgatcagaaa ccgactggtt 2280 tgtcacactc tggttagagg aggggtcacc acgatgggtg agggggtggt gggtgtttat 2340 acaagagtct gggactccaa agacgatatt acgacgagac gacttatcca cgtactttgt 2400 ttcccaaacc cacgcaactc cacctttagt ccacacaaac tagtacgaga cacccggatt 2460 caaggtcgtg acc 2473 <210> 115 <211> 2029 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 115 ggaccgaagc atactgcaag acttgtaggc ccatgaatta cccgcttagg caatgggtag 60 acctttcctt gccgcgtgga acaaaccgcc atgagtgttt tacgtttacg gtcggcgacc 120 ggagtcacag cgcgaccaac ggggccaggc aaaggcccat aggcttttcc atcggcacgc 180 gaccgattcc atcaagtgtt aggtgcaatc cccattcggg acggacacgc gagagcgtta 240 gtcggcgcgt gcgcgggact atttagcgaa ggtcgccgat cgtttgcccg taaaactaac 300 tggcaaaagc ctaagtgctg tttcgggtgc aatggtataa cttcccacat ttagccgaat 360 agcgccaaca gcgttattat ttggaaccaa gcagtgggcg atgtcagcga caattccact 420 ttggtagagg tcgttctttt gtagaaaaca atctaacata actatgacac tttcggtaaa 480 ttcaccacct ttaccagggt cgtaagtggt caagccacgc cgaccacttc ataagcattg 540 gtggaaatcg catcgacaca ataaaagtac accaaccagc agaaaacagg tacatataca 600 gtttaacgac ctggatctaa gatgactaca gtagcatagc catagaacat ggcactgaaa 660 tatcgatggg cgtaaaagtg aacaacgcag acgatggatt acgacgccaa gccactataa 720 atgctaattg agtcgaagaa ggtactcctt tttggaacac agatattgca gaagcgggaa 780 tgaggttgat attacatcaa gtatcaacag acgacctcag aagggtggtc ttttaaaatg 840 acaacgtaca ttaagatgca gacaaacact actatgctgg agtgttgtat taattcactc 900 tagagtaatg gatcacggtg ccagaaacta aggtggtgta ggactagatg caagtaacaa 960 cggtcgtact aaaatcagct acgcgatcgc cgacttagaa ggctgagatg gagaaacttg 1020 tctactggca gacctcacta tcttttagga aggtcacgaa gcggctggag tgatggctag 1080 tctcagccac tgtcaacggg aagcgcgacg tttatggtca atataaacta gcagtttaat 1140 ccgtatggcg atcttagcgg atgttacagg atggagaaga tgacaaagta gaagctagac 1200 aactatatca aatcgttgtg tggtcttaaa ccgacacagt agcattcatc gttggatgtg 1260 gaaggtcaca gcctcgttgg aggagatact acggtcccgc ttttcgtggg tgacaaaggt 1320 cgcaaaggtt tccttaggga ggtcgtttcc cggatcttta tctacatctc ctgggtgaag 1380 gggatgatct ttctctacat gtcctgggtg gaggggagtt tccttgtatg ggtggaaccc 1440 aacccgccgg tgagggtgag ttacggtcgg cggtcatatg tgggtatgac gggtatgacg 1500 tcataaatag tcgttgtcaa aatgggaggt ggaagtcctt actgtagtca cccgtgaagg 1560 tggagaactg gcaggacatc tctaggtcac ctaaacgtgc gaagtgtgcg aaattaccac 1620 cgtaaattcg tcctactgaa agtgcttacg catcttgaga gagattgcag agagccaggt 1680 caaatacatt gagaaatctg tatatgtact ttggtaacgc tctaaggtag gtacacgtta 1740 gtgatataaa aagaaggcga cgacgaactt aaattgttag agcagtttat gggtggtagg 1800 ttacatctat ttacttcaaa acgcggtgcc ctagcccaca agcattgctt tgtaaaattg 1860 ttgaaggtaa cttgtaaaag ccgaacatga acgatggata gtagaccggg aagaccaacg 1920 ttttttcact actccgaaag aacacactga agggaacggt gtaggttcgg tgagtccggg 1980 actaggtgtc catgtcggag acctcagtgg tgacgtcctc gtagtatag 2029 <210> 116 <211> 552 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 116 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggggccag gcaaaggccc ataggctttt ccatcggcac gcgaccgatt 180 ccatcaagta tagccacggt cgtaacttcg acggcggcaa tagtttatgg cgctaacacc 240 tttccatcgc tgagtccaaa cgtatgacgt gaggtgttag gtgcaatccc cattcgggac 300 ggacacgcga gtaaaggtct aacatgtttc aactttccag ttagtcgccg tcctatattg 360 gcaagaccgg atggccgtca accagttcca ttctgactat ttgccacatt gattaaagaa 420 gtaggagcgt tagtcggcgc gtgcgcggga ctatttagcg aaggtcgccg tatcgcgggt 480 ggcggcgttt ccgccataac aacaagcgtt taaggtcaga gtccgcgacg aatgaaacgc 540 cgtgcaagcg gc 552 <210> 117 <211> 533 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 117 ggcccatgaa ttacccgctt aggcaatggg tagacctttc cttgccgcgt ggaacaaacc 60 gccatgagtg ttttacgttt acggtcggcg accggagtca cagcgcgacc aacggggcca 120 gggaggaagc cttttagcca tcgctatgcc gccgctcaaa cgcgccgggt gcaaaagccc 180 agagcaaagg cccataggct tttccatcgg cacgcgaccg attccatcaa gtgttaggtg 240 caatccccat tcgggacgga cacgcgagag cgttagtcgg cgcgtgcgcg ggactattta 300 gcgaaggtcg ccgatcgttt gcccgtaaaa ctaactggca aaagcctaag tgctgtttcg 360 ggtgcaatgg tataacttcc cacatttagc cgaatagcgc caacagcgtt attatttgga 420 accaagcagt gggcgatgtc agcgacaatt ccactttggt agaggtcgtt cttttgtaga 480 aaacaatcta acataactat gacactttcg gtaaattcac cacctttacc agg 533 <210> 118 <211> 2085 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 118 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggggccag gcaaaggccc ataggctttt ccatcggcac gcgaccgatt 180 ccatcaagtg ttaggtgcaa tccccattcg ggacggacac gcgagagcgt tagtcggcgc 240 gtgcgcggga ctatttagcg aaggtcgccg atcgtttgcc cgtaaaacta actggcaaaa 300 gcctaagtgc tgtttcgggt gcaatggtat aacttcccac atttagccga atagcgccaa 360 cagcgttatt atttggaacc aagcagtggg cgatgtcagc gacaattcca ctttggtaga 420 ggtcgttctt ttgtagaaaa caatctaaca taactatgac actttcggta aattcaccac 480 ctttaccagg gtcgtaagtg gtcaagccac gccgaccact tcataagcat tggtggaaat 540 cgcatcgaca caataaaagt acaccaacca gcagaaaaca ggtacatata cagtttaacg 600 acctggatct aagatgacta cagtagcata gccatagaac atggcactga aatatcgatg 660 ggcgtaaaag tgaacaacgc agacgatgga ttacgacgcc aagccactat aaatgctaat 720 tgagtcgaag aaggtactcc tttttggaac acagatattg cagaagcggg aatgaggttg 780 atattacatc aagtatcaac agacgacctc agaagggtgg tcttttaaaa tgacaacgta 840 cattaagatg cagacaaaca ctactatgct ggatgtgaaa atgtcatatg tgcggcaaaa 900 atagacgaca tttaagaacc tagcattata ggatggagtg ttgtattaat tcactctaga 960 gtaatggatc acggtgccag aaactaaggt ggtgtaggac tagatgcaag taacaacggt 1020 cgtactaaaa tcagctacgc gatcgccgac ttagaaggct gagatggaga aacttgtcta 1080 ctggcagacc tcactatctt ttaggaaggt cacgaagcgg ctggagtgat ggctagtctc 1140 agccactgtc aacgggaagc gcgacgttta tggtcaatat aaactagcag tttaatccgt 1200 atggcgatct tagcggatgt tacaggatgg agaagatgac aaagtagaag ctagacaact 1260 atatcaaatc gttgtgtggt cttaaaccga cacagtagca ttcatcgttg gatgtggaag 1320 gtcacagcct cgttggagga gatactacgg tcccgctttt cgtgggtgac aaaggtcgca 1380 aaggtttcct tagggaggtc gtttcccgga tctttatcta catctcctgg gtgaagggga 1440 tgatctttct ctacatgtcc tgggtggagg ggagtttcct tgtatgggtg gaacccaacc 1500 cgccggtgag ggtgagttac ggtcggcggt catatgtggg tatgacgggt atgacgtcat 1560 aaatagtcgt tgtcaaaatg ggaggtggaa gtccttactg tagtcacccg tgaaggtgga 1620 gaactggcag gacatctcta ggtcacctaa acgtgcgaag tgtgcgaaat taccaccgta 1680 aattcgtcct actgaaagtg cttacgcatc ttgagagaga ttgcagagag ccaggtcaaa 1740 tacattgaga aatctgtata tgtactttgg taacgctcta aggtaggtac acgttagtga 1800 tataaaaaga aggcgacgac gaacttaaat tgttagagca gtttatgggt ggtaggttac 1860 atctatttac ttcaaaacgc ggtgccctag cccacaagca ttgctttgta aaattgttga 1920 aggtaacttg taaaagccga acatgaacga tggatagtag accgggaaga ccaacgtttt 1980 ttcactactc cgaaagaaca cactgaaggg aacggtgtag gttcggtgag tccgggacta 2040 ggtgtccatg tcggagacct cagtggtgac gtcctcgtag tatag 2085 <210> 119 <211> 885 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 119 gtatatgtac tttggtaacg ctctaaggta ggtacacgtt agtgatataa aaagaaggcg 60 acgacgaact taaattgtta gagcagttta tgggtggtag gttacatcta tttacttcaa 120 aacgcggtgc cctagcccac aagcattgct ttgtaaaatt gttgaaggta acttgtaaaa 180 gccgaacatg aacgatggat agtagaccgg gaagaccaac gttttttcac tactccgaaa 240 gaacacactg aagggaacgg tgtaggttcg gtgagtccgg gactaggtgt ccatgtcgga 300 gacctcagtg gtgacgtcct cgtagtatag ctgctcgcat gaccagctat atctggaaca 360 tcgctggtgg gtcttatggt cgcttttgtg ggaaccatca tagtcgcaag tctggccttc 420 ggtatggaac tcgtcgtccc gactacaacc atagctagtc tcggtctcgg ctttgttgca 480 atgcccttcg ttactacttc aacggtcgtg gtgccggtca aaccgctcgc taaagcaagc 540 gaagaaacag ctaaacttag ttgtgtcgaa acatcctaag tggtcgggtt gctcgtggag 600 catgcagcta gcccagaacc tttagcttgt gtggtcttcg tctcggtagt cagaaccagg 660 tcggtctgaa tggttggact cgccacatca actaggtcag ctattgaagt gccaactaaa 720 ggtcgtattg aagcaatcgg tcctatggca agtcaagtga cagtcgtagg tatggcactt 780 gccgcgcctc cagaactcgg tcaagctacc tcataactcg gaagtcgact gctctaaagc 840 gaagttgtca aacgaacagc tatgccttcg agtaaagcaa ggtgc 885 <210> 120 <211> 2474 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 120 gcccatgaat tacccgctta ggcaatgggt agacctttcc ttgccgcgtg gaacaaaccg 60 ccatgagtgt tttacgttta cggtcggcga ccggagtcac agcgcgacca acggggccag 120 gcaaaggccc ataggctttt ccatcggcac gcgaccgatt ccatcaagtg ttaggtgcaa 180 tccccattcg ggacggacac gcgagagcgt tagtcggcgc gtgcgcggga ctatttagcg 240 aaggtcgccg atcgtttgcc cgtaaaacta actggcaaaa gcctaagtgc tgtttcgggt 300 gcaatggtat aacttcccac atttagccga atagcgccaa cagcgttatt atttggaacc 360 aagcagtggg cgatgtcagc gacaattcca ctttggtaga ggtcgttctt ttgtagaaaa 420 caatctaaca taactatgac actttcggta aattcaccac ctttaccagg gtcgtaagtg 480 gtcaagccac gccgaccact tcataagcat tggtggaaat cgcatcgaca caataaaagt 540 acaccaacca gcagaaaaca ggtacatata cagtttaacg acctggatct aagatgacta 600 cagtagcata gccatagaac atggcactga aatatcgatg ggcgtaaaag tgaacaacgc 660 agacgatgga ttacgacgcc aagccactat aaatgctaat tgagtcgaag aaggtactcc 720 tttttggaac acagatattg cagaagcggg aatgaggttg atattacatc aagtatcaac 780 agacgacctc agaagggtgg tcttttaaaa tgacaacgta cattaagatg cagacaaaca 840 ctactatgct ggagtgttgt attaattcac tctagagtaa tggatcacgg tgccagaaac 900 taaggtggtg taggactaga tgcaagtaac aacggtcgta ctaaaatcag ctacgcgatc 960 gccgacttag aaggctgaga tggagaaact tgtctactgg cagacctcac tatcttttag 1020 gaaggtcacg aagcggctgg agtgatggct agtctcagcc actgtcaacg ggaagcgcga 1080 cgtttatggt caatataaac tagcagttta atccgtatgg cgatcttagc ggatgttaca 1140 ggatggagaa gatgacaaag tagaagctag acaactatat caaatcgttg tgtggtctta 1200 aaccgacaca gtagcattca tcgttggatg tggaaggtca cagcctcgtt ggaggagata 1260 ctacggtccc gcttttcgtg ggtgacaaag gtcgcaaagg tttccttagg gaggtcgttt 1320 cccggatctt tatctacatc tcctgggtga aggggatgat ctttctctac atagattgca 1380 gagagccagg tcaaatacat tgagaaatct gtatatgtac tttggtaacg ctctaaggta 1440 ggtacacgtt agtgatataa aaagaaggcg acgacgaact taaattgtta gagcagttta 1500 tgggtggtag gttacatcta tttacttcaa aacgcggtgc cctagcccac aagcattgct 1560 ttgtaaaatt gttgaaggta acttgtaaaa gccgaacatg aacgatggat agtagaccgg 1620 gaagaccaac gttttttcac tactccgaaa gaacacactg aagggaacgg tgtaggttcg 1680 gtgagtccgg gactaggtgt ccatgtcgga gacctcagtg gtgacgtcct cgtagtatag 1740 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 1800 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 1860 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 1920 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 1980 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 2040 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 2100 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 2160 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 2220 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 2280 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 2340 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 2400 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 2460 taaagcaagg tgcg 2474 <210> 121 <211> 546 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 121 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acgggcaaag gcccataggc ttttccatcg gcacgcgacc gattccatca 180 agtatagcca cggtcgtaac ttcgacggcg gcaatagttt atggcgctaa cacctttcca 240 tcgctgagtc caaacgtatg acgtgaggtg ttaggtgcaa tccccattcg ggacggacac 300 gcgagtaaag gtctaacatg tttcaacttt ccagttagtc gccgtcctat attggcaaga 360 ccggatggcc gtcaaccagt tccattctga ctatttgcca cattgattaa agaagtagga 420 gcgttagtcg gcgcgtgcgc gggactattt agcgaaggtc gccgtatcgc gggtggcggc 480 gtttccgcca taacaacaag cgtttaaggt cagagtccgc gacgaatgaa acgccgtgca 540 agcggc 546 <210> 122 <211> 229 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 122 gtaccaaagc cgcttgcacg gcgtttcatt cgtcgcggac tctgacctta aacgcttgtt 60 gttatggcgg aaacgccgcc acccgcgata cggcgacctt cgctaaatag tcccgcgcac 120 gcgccgacta acgctcctac ttctttaatc aatgtggcaa atagtcagaa tggaactggt 180 tgacggccat ccggtcttgc caatatagga cggcgactaa ctggaaagt 229 <210> 123 <211> 1435 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 123 ggcaagacga atctgcgtca acgctaaagg ccgtcaaaaa gcaagagggc gtcgcgcctg 60 ggaaaaaggt agtcaagaaa ccatcaagct taaatgtcgg gaaattgggt ttgcgccgcc 120 ttcaggggca gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact 180 cctcgggtca accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag 240 caaggtgccc aaagtagact gagcgacagt cgaaaccagc cccaatgaac aagacgccat 300 tgcaaaacgt ctatacgcta cggtcaaaga cgcttccaca ccacatgttg tgctcgccat 360 ttcaactagt gcagttgcta aagtgccaac taaggttcgt cttgaagcaa tcggtagtat 420 ctgcagtcaa gtcgtagtcg taggtatggc acttgtcgtg cgtctaggac gcgttgaagc 480 tatcttattg cccgaaggag ccgaaagtct gggacacgtc gtgggacgta tcgttaggac 540 tatgggacta gtcgtcttcg gtcttacaaa tgttgcaatg ccctgtgagc tacttatgaa 600 acatgaatgg tcggtcttgt ggtcgctttt gtcgaaatca ccgaagtgcg tataattgat 660 gaacgagtcg aagtcgaagt tgttcaacaa gagagttagg tagtgccgct tgaaaaagta 720 atagtgcaaa taagaaaaag aagcgaaacg gcattcgttt aacaaaacgt cacatattta 780 ggtatagtag attgcgttca caaagaaatt taaatttaat ttaccatttc gtcattgtat 840 atgcggtaaa cgtaaattta cacatatgac acgaactttg agttttatta gcctgcctaa 900 ttgttggtaa caatgtaatg gaaattggcg atgactatgg ggaaagagca atcaactcac 960 cataaatttg tgaacgcagt taagtaattg gcatcgcgcg cctaatcgtt tacattcgca 1020 tggaacaaga agaaagagac ctattcgtgg gagtcaatgg ctatgtggaa aaacatgaac 1080 tgccaaattc ggctaaccaa ccagaaatgg tcatggaaaa ttcttgcgtc aaagttcgcg 1140 gtgacgttta cgaagaagct aaacttgtgc ttgtttagaa agttaggtgc gtcctggttt 1200 tagtaaattt agaaaaccat tttgttgttt gcgacagtta aataaaactt aacgacaaag 1260 aagtgacttg cgtcgtagat taactggaaa ttaaagtggt atctaaaatg gggtttgccg 1320 atgcggtctt caaagtctaa aattcttgaa gtttgcgttt ggtcgcaaaa attatggatt 1380 tagtcgatta ttgcctacta agcagcagtt tagaagtttt taagatagtt acgct 1435 <210> 124 <211> 574 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 124 ggggcagcga tgggggcgac aattgtggac cgtatggact ccattatgct gggactcctc 60 gggtcaaccg ctctaaagcg aagttgttgg acaaacagtt atgcgtaact gtaaagcaag 120 gtgcccaaag tagactgagc gacagtcgaa accagcccca atgaacaaga cgccattgca 180 aaacgtctat acgctacggt caaagacgct tccacaccac atggactatg ggactagtcg 240 tcttcggtct tacaaatgtt gcaatgccct gtgagctact tatgaaacat gaatggtcgg 300 tcttgtggtc gcttttgtcg aaatcaccga agtgcgtata attgatgaac gagtcgaagt 360 cgaagttgtt caacaagaga gttaggtagt gccgcttgaa aaagtaatag tgcaaataag 420 aaaaagaagc gaaacggcat tcgtttaaca aaacgtcaca tatttaggta tagtagattg 480 cgttcacaaa gaaatttaaa tttaatttac catttcgtca ttgtatatgc ggtaaacgta 540 aatttacaca tatgacacga actttgagtt ttat 574 <210> 125 <211> 1969 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 125 gtgggtttat atcgcgtatc catccaactt aactgaaacg cgaagcagta gacagtgttg 60 atcgagattt gcgaagggaa acagtaaaag tccagggtgt cgttaattac gacaaaacta 120 aagactgtat agttagaagg atcaattggg ctgatttaga aatggtataa gttaaagaaa 180 tggaaactta ttcgacaaaa agaaaaactc atgatgtata gatggaaact gccataatgt 240 cgattcgagt tatattttta gattttggta aaatatccaa catgtattaa cgaaattttg 300 aagtatagat caaactattc ttcaggtaca gcatcaagca ttcggtagca taagccaaac 360 aagtctttga agggttggac atagcgagtt gtgtagaaag ttacgaagac ttcaacagaa 420 aaagagttta aaaggaccac gtatgccaaa tcgacaacaa tatagtcgtt ctattctgcg 480 atatgtaaga tagagacaca tcggcaggta gcatagaact tgggtcaatt attatttaag 540 tgcggtaaca aacaaactta gggaatatta ttgtgctaca gcaaatgcct tgaaaagtcg 600 agaaagacta ctatactcgt caagttcgta aagacttaac agtaattgta atgcaaatac 660 ttagcgagat tgattccttc ccagtagtca tctttccttt agatattaga acagcaattt 720 tagattttgt acatagtgga gaaaaggacg tcattttaat ggatgaacca gacgttaacg 780 tcatttactt ggtcgttgat ttcgaagact cagtcatcaa aattgtagct tcgctagcta 840 atgtgcttaa aatcatcata gtcaaagtgt acgtggtcgt ggtaataaac aaccttcatt 900 actgtacaaa agtgccagtt tgttacatta tcgaacgctg tatagaggta tcatttcacc 960 tatttgacaa ctaggtgata aaccttcggt cgctagacgt tgttgttatt gaaaaaatgg 1020 aagataatct atacttaatc caaaacaacc cagaccaagc tcgtatataa atggaagtcg 1080 tcaaaattgg tttcgttcaa ataagttggg actgatgaat cagttttctg aacttcaccg 1140 ctattagcgt cctagttgtc tacataatta gtatcattat tgtagtagtt taaatcgcta 1200 acaaccaaaa aatccttgac atgggtaacg acgttattgg ggaaccaatg ctcagtgttg 1260 gtgaaagaat cgttaggtcg aactattggt acgttctaat aaaagacgac atagaaattc 1320 gttacataat caacgtcact actaagggcg tattgcaaat tagttcataa attattaaca 1380 gtttgcacct aatagttcta gtccttaaaa aggtagaaga cgtaggtaat gtcagttgcc 1440 tcaatgtagt caggtaaaaa gtatatgtgc tgatggcaaa cttcatcttt gtgagagttg 1500 agcatgtata acaacatcac tcagtataac cgcttggtta cacaattagt atcatgccta 1560 tagaacgcct tatcattcag gtcgtcgtga tagtgaaggg gatcaaagtc aaaatctact 1620 ttgtcataac tctttacggt atcccaatgc ttggtataag tgcttaagaa attgtttaaa 1680 cagaaactca agtataagcg aaaacaagtt acctggaaaa agaagaagtg cgtataattg 1740 atgaacgagt cgaagtcgaa gttgttcaac aagagagtta ggtagtgccg cttgaaaaag 1800 taatagtgca aataagaaag taagactgta tcggagacga ctttttaata gtagtaattc 1860 tcggtacaac taaaccagta atttcagaca ctgaaataga ggacttcgtt aggtacgaag 1920 caagttaact aaatgacaat taaattgagg gtaaatatca caaagaaat 1969 <210> 126 <211> 1537 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 126 gcttaaatgt cgggaaattg ggtttgcgcc gccttcaggg gcagcgatgg gggcgacaat 60 tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc taaagcgaag 120 ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag actgagcgac 180 agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg ctacggtcaa 240 agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc gcacaagtcg 300 gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc ctcacgagta 360 gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg cttggtatgg 420 ctattgtcgg cctggatgca attgtgctcg ccatttcaac tagtgcagtt gctaaagtgc 480 caactaaggt tcgtcttgaa gcaatcggta gtatctgcag tcaagtcgta gtcgtaggta 540 tggcacttgt cgtgcgtcta ggacgcgttg aagctatctt attgcccgaa ggagccgaaa 600 gtctgggaca cgtcgtggga cgtatcgtta ggactatggg actagtcgtc ttcggtctta 660 caaatgttgc aatgccctgt gagctactta tgaaacatga atggtcggtc ttgtggtcgc 720 ttttgtcgaa atcaccgaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca 780 acaagagagt taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagcgaaac 840 ggcattcgtt taacaaaacg tcacatattt aggtatagta gattgcgttc acaaagaaat 900 ttaaatttaa tttaccattt cgtcattgta tatgcggtaa acgtaaattt acacatatga 960 cacgaacttt gagttttatt agcctgccta attgttggta acaatgtaat ggaaattggc 1020 gatgactatg gggaaagagc aatcaactca ccataaattt gtgaacgcag ttaagtaatt 1080 ggcatcgcgc gcctaatcgt ttacattcgc atggaacaag aagaaagaga cctattcgtg 1140 ggagtcaatg gctatgtgga aaaacatgaa ctgccaaatt cggctaacca accagaaatg 1200 gtcatggaaa attcttgcgt caaagttcgc ggtgacgttt acgaagaagc taaacttgtg 1260 cttgtttaga aagttaggtg cgtcctggtt ttagtaaatt tagaaaacca ttttgttgtt 1320 tgcgacagtt aaataaaact taacgacaaa gaagtgactt gcgtcgtaga ttaactggaa 1380 attaaagtgg tatctaaaat ggggtttgcc gatgcggtct tcaaagtcta aaattcttga 1440 agtttgcgtt tggtcgcaaa aattatggat ttagtcgatt attgcctact aagcagcagt 1500 ttagaagttt ttaagatagt tacgctagaa gaccggg 1537 <210> 127 <211> 1088 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 127 gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgggtca 60 accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag caaggtgccc 120 aaagtagact gagcgacagt cgaaaccagc cccaatgaac aagacgccat tgcaaaacgt 180 ctatacgcta cggtcaaaga cgcttccaca ccacatgctc tcatgtagcc taccttacga 240 agaaatcgca caagtcggtc gcccagcggt ggcatatgtc ttgcctaacg tttctagacc 300 gatcagcctc acgagtagcc tgcttgtggt gtttatagtt tacgctagtc caatctgtat 360 cgtgccgctt ggtatggcta ttgtcggcct ggatgcaatt gtgctcgcca tttcaactag 420 tgcagttgct aaagtgccaa ctaaggttcg tcttgaagca atcggtagta tctgcagtca 480 agtcgtagtc gtaggtatgg cacttgtcgt gcgtctagga cgcgttgaag ctatcttatt 540 gcccgaagga gccgaaagtc tgggacacgt cgtgggacgt atcgttagga ctatgggact 600 agtcgtcttc ggtcttacaa atgttgcaat gccctgtgag ctacttatga aacatgaatg 660 gtcggtcttg tggtcgcttt tgtcgaaatc accgaagtgc gtataattga tgaacgagtc 720 gaagtcgaag ttgttcaaca agagagttag gtagtgccgc ttgaaaaagt aatagtgcaa 780 ataagaaaaa gaagcgaaac ggcattcgtt taacaaaacg tcacatattt aggtatagta 840 gattgcgttc acaaagaaat ttaaagtctt gtaattatgg tggtcgattt attggtagta 900 tatttggaaa tagcatgcaa ttttggaatg gacgaagaac ctgacatctg agctattgta 960 agtttaattt accatttcgt cattgtatat gcggtaaacg taaatttaca catatgacac 1020 gaactttgag ttttattagc ctgcctaatt gttggtaaca atgtaatgga aattggcgat 1080 gactatgg 1088 <210> 128 <211> 545 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 128 gaatattgtt tcaatggtcg tggcgctggt cagactgctc gctaaagtcg tcgaagaaac 60 agctagacca tctattctct gaacttccta agtggtcggg ttggtcgtgg cgctcatagt 120 tagcccagaa cgtccagttt atgtggtggt gatcttggtt gccagactca gtatggtcaa 180 aatagttgtg ctcgccattt caactagtgc agttgctaaa gtgccaacta aggttcgtct 240 tgaagcaatc ggtagtatct gcagtcaagt cgtagtcgta ggtatggcac ttgtcgtgcg 300 tctaggacgc gttgaagcta tcttattgcc cgaaggagcc gaaagtctgg gacacgtcgt 360 gggacgtatc gttaggacta tgggactagt cgtcttcggt cttacaaatg ttgcaatgcc 420 ctgtgagcta cttatgaaac atgaatggtc ggtcttgtgg tcgcttttgt cgaaatcacc 480 gaagtgcgta taattgatga acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta 540 gtgcc 545 <210> 129 <211> 574 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 129 gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgggtca 60 accgctctaa agcgaagttg ttggacaaac agttatgcgt aactgtaaag caaggtgccc 120 aagtagactc ttactaattt gggaacgacg gaaagtcgct accttcatcg gtatggcttt 180 cgctaaagtc ggtcaaatgg aagaaggtag tcgaagtact gttgaaagag tagactgagc 240 gacagtcgaa accagcccca atgaacaaga cgccattgca aaacgtctat acgctacggt 300 caaagacgct tccacaccac atgctctcat gtagcctacc ttacgaagaa atcgcacaag 360 tcggtcgccc agcggtggca tatgtcttgc ctaacgtttc tagaccgatc agcctcacga 420 gtagcctgct tgtggtgttt atagtttacg ctagtccaat ctgtatcgtg ccgcttggta 480 tggctattgt cggcctggat gcaattgtgc tcgccatttc aactagtgca gttgctaaag 540 tgccaactaa ggttcgtctt gaagcaatcg gtag 574 <210> 130 <211> 377 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 130 gcctatgcct cgtatcatct atggtcgtag gtaaagcaat agcggaaaca ttggatgtga 60 cttcatcagg actgctcgta tagtgagcta cttatgaaac atgaatggtc ggtcttgtgg 120 tcgcttttgt cgaaatcacc gaagtgcgta taattgatga acgagtcgaa gtcgaagttg 180 ttcaacaaga gagttaggta gtgccgcttg aaaaagtaat agtgcaaata agaaaaagaa 240 gcgaaacggc attcgtttaa caaaacgtca catatttagg tatagtagat tgcgttcaca 300 aagaaattta aatttaattt accatttcgt cattgtatat gcggtaaacg taaatttaca 360 catatgacac gaacttt 377 <210> 131 <211> 485 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 131 gggcgacaat tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc 60 taaagcgaag ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag 120 actgagcgac agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg 180 ctacggtcaa agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc 240 gcacaagtcg gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc 300 ctcacgagta gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg 360 cttggtatgg ctattgtcgg cctggatgca agcactaacg tggtcatcag actggtctgc 420 gctataatct acgcaataga ggcaccagcg gtcgttgaag ccgaagacgc tatttcggtg 480 gcttg 485 <210> 132 <211> 1163 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 132 gtgctcgcca tttcaactag tgcagttgct aaagtgccaa ctaaggttcg tcttgaagca 60 atcggtagta tctgcagtca agtcgtagtc gtaggtatgg cacttgtcgt gcgtctagga 120 cgcgttgaag ctatcttatt gcccgaagga gccgaaagtc tgggacacgt cgtgggacgt 180 atcgttagga ctatgggact agtcgtcttc ggtcttacaa atgttgcaat gccctgtgag 240 ctacttatga aacatgaatg gtcggtcttg tggtcgcttt tgtcgaaatc accgaagtgc 300 gtataattga tgaacgagtc gaagtcgaag ttgttcaaca agagagttag gtagtgccgc 360 ttgaaaaagt aatagtgcaa ataagaaagt aagactgtat cggagacgac tttttaatag 420 tagtaattct cggtacaact aaaccagtaa tttcagacac tgaaatagag gacttcgtta 480 ggtacgaagc aagttaacta aatgacaatt aaattgaggg taaatatcac aaagaaatca 540 tggaagattt cgaccgaata caggaaaatt tagattaaag gtttgtcgtc gttgaaataa 600 gaagaagcga aacggcattc gtttaacaaa acgtcacata tttaggtata gtagattgcg 660 ttcacaaaga aatttaaatt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa 720 tttacacata tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt 780 aatggaaatt ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg 840 cagttaagta attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag 900 agacctattc gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa 960 ccaaccagaa atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga 1020 agctaaactt gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa 1080 ccattttgtt gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt 1140 agattaactg gaaattaaag tgg 1163 <210> 133 <211> 454 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 133 ggtagttgtt gaaaaagtag gaatatctag cctagatgtc gtttcaggcg aaggactcta 60 aggtcttaaa atcgttgggt caaccgctct aaagcgaagt tgttggacaa acagttatgc 120 gtaactgtaa agcaaggtgc ccaaagtaga ctgagcgaca gtcgaaacca gccccaatga 180 acaagacgcc attgcaaaac gtctatacgc tacggtcaaa gacgcttcca caccacatgg 240 tatagtggac gccgttagcc tcatgtgtct cactcaaaga ccttgcgtca cggtcatcct 300 ctttgtcagc aacgtagaaa tctccggtct gtcgatacct tgcgtaaagc tctcatgtag 360 cctaccttac gaagaaatcg cacaagtcgg tcgcccagcg gtggcatatg tcttgcctaa 420 cgtttctaga ccgatcagcc tcacgagtag cctg 454 <210> 134 <211> 1528 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 134 gcttaaatgt cgggaaattg ggtttgcgcc gccttcaggg gcagcgatgg gggcgacaat 60 tgtggaccgt atggactcca ttatgctggg actcctcggg tcaaccgctc taaagcgaag 120 ttgttggaca aacagttatg cgtaactgta aagcaaggtg cccaaagtag actgagcgac 180 agtcgaaacc agccccaatg aacaagacgc cattgcaaaa cgtctatacg ctacggtcaa 240 agacgcttcc acaccacatg ctctcatgta gcctacctta cgaagaaatc gcacaagtcg 300 gtcgcccagc ggtggcatat gtcttgccta acgtttctag accgatcagc ctcacgagta 360 gcctgcttgt ggtgtttata gtttacgcta gtccaatctg tatcgtgccg cttggtatgg 420 ctattgtcgg cctggatgca attgtgctcg ccatttcaac tagtgcagtt gctaaagtgc 480 caactaaggt tcgtcttgaa gcaatcggta gtatctgcag tcaagtcgta gtcgtaggta 540 tggcacttgt cgtgcgtcta ggacgcgttg aagctatctt attgcccgaa ggagccgaaa 600 gtctgggaca cgtcgtggga cgtatcgtta ggactatggg actagtcgtc ttcggtctta 660 caaatgttgc aatgccctgt gagctactta tgaaacatga atggtcggtc ttgtggtcgc 720 ttttgtcgaa atcaccgaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca 780 acaagagagt taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagaagcga 840 aacggcattc gtttaacaaa acgtcacata tttaggtata gtagattgcg ttcacaaaga 900 aatttaaatt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 960 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 1020 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 1080 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 1140 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 1200 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 1260 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 1320 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 1380 gaaattaaag tggtatctaa aatggggttt gccgatgcgg tcttcaaagt ctaaaattct 1440 tgaagtttgc gtttggtcgc aaaaattatg gatttagtcg attattgcct actaagcagc 1500 agtttagaag tttttaagat agttacgc 1528 <210> 135 <211> 1311 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 135 gtgagctact tatgaaacat gaatggtcgg tcttgtggtc gcttttgtcg aaatcaccgg 60 aaacattaaa tcaaagaagt ttatcaacca attagtacag tggcatgtta agtaaaacag 120 aaagaacgcc aagtggcaac aaatgacgta attatttaag caaaaagcgt aaacgcttac 180 aaaggctatt taaatatcag taaatcgttg gaaattgggt atgtgctgcc ttcatacgca 240 aaaatggatg cgacagaaac gagttgtatg gcctccacta cgctcgcctg ccgcggaccc 300 taaacgacag tcgtttgccc aactacggct acggcatcgc tcagaacttc gggtcttggc 360 ccacctttct gacgcctagt caacgtgcgt cgcctcagtc ttggacaaaa agaagaaata 420 tcaattgcaa aaataactat aggtacttct ttgaagaaac caaacgtgag tttctaacac 480 gactaaatcc atttgatctt tgagaaagtc gaagacgaat gaagtgcgta taattgatga 540 acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta gtgccgcttg aaaaagtaat 600 agtgcaaata agaaaaagaa gcgaaacggc attcgtttaa caaaacgtca catatttagg 660 tatagtagat tgcgttcaca aagaaattta aatttaattt accatttcgt cattgtatat 720 gcggtaaacg taaatttaca catatgacac gaactttgag ttttattagc ctgcctaatt 780 gttggtaaca atgtaatgga aattggcgat gactatgggg aaagagcaat caactcacca 840 taaatttgtg aacgcagtta agtaattggc atcgcgcgcc taatcgttta cattcgcatg 900 gaacaagaag aaagagacct attcgtggga gtcaatggct atgtggaaaa acatgaactg 960 ccaaattcgg ctaaccaacc agaaatggtc atggaaaatt cttgcgtcaa agttcgcggt 1020 gacgtttacg aagaagctaa acttgtgctt gtttagaaag ttaggtgcgt cctggtttta 1080 gtaaatttag aaaaccattt tgttgtttgc gacagttaaa taaaacttaa cgacaaagaa 1140 gtgacttgcg tcgtagatta actggaaatt aaagtggtat ctaaaatggg gtttgccgat 1200 gcggtcttca aagtctaaaa ttcttgaagt ttgcgtttgg tcgcaaaaat tatggattta 1260 gtcgattatt gcctactaag cagcagttta gaagttttta agatagttac g 1311 <210> 136 <211> 459 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 136 ggttcgtctt gaagcaatcg gtagtatctg cagtcaagtc gtagtcgtag gtatggcact 60 tgtcgtgcgt ctaggacgcg ttgaagctat cttattgccc gaaggagccg aaagtctggg 120 acacgtcgtg ggacgtatcg ttaggactat gggactagtc gtcttcggtc ttacaaatgt 180 tgcaatgccc tgtgagctac ttatgaaaca tgaatggtcg gtcttgtggt cgcttttgtc 240 gaaatcaccg gacaaaaaga agaaatatca attgcaaaaa taactatagg tacttctttg 300 aagaaaccaa acgtgagttt ctaacacgac taaatccatt tgatctttga gaaagtcgaa 360 gacgaatgaa gtgcgtataa ttgatgaacg agtcgaagtc gaagttgttc aacaagagag 420 ttaggtagtg ccgcttgaaa aagtaatagt gcaaataag 459 <210> 137 <211> 783 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 137 ggggaaccaa tgctcagtgt tggtgaaaga atcgttaggt cgaactattg gtacgttcta 60 ataaaagacg acatagaaat tcgttacata atcaacgtca ctactaaggg cgtattgcaa 120 attagttcat aaattattaa cagtttgcac ctaatagttc tagtccttaa aaaggtagaa 180 gacgtaggta atgtcagttg cctcaatgta gtcaggtaaa aagtatatgt gctgatggca 240 aacttcatct ttgtgagagt tgagcatgta taacaacatc actcagtata accgcttggt 300 tacacaatta gtatcatgcc tatagaacgc cttatcattc aggtcgtcgt gatagtgaag 360 gggatcaaag tcaaaatcta ctttgtcata actctttacg gtatcccaat gcttggtata 420 agtgcttaag aaattgttta aacagaaact caagtataag cgaaaacaag ttacctggaa 480 aaagaagaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca acaagagagt 540 taggtagtgc cgcttgaaaa agtaatagtg caaataagaa aaagaagcga aacggcattc 600 gtttaacaaa acgtcacata tttaggtata gtagattgcg ttcacaaaga aatttaaatt 660 taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata tgacacgaac 720 tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt ggcgatgact 780 atg 783 <210> 138 <211> 531 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 138 gaatattgtt tcaatggtcg tggcgctggt cagactgctc gctaaagtcg tcgaagaaac 60 agctagacca tctattctct gaacttccta agtggtcggg ttggtcgtgg cgctcatagt 120 tagcccagaa cgtccagttt atgtggtggt gatcttggtt gccagactca gtatggtcaa 180 aatagttgtg ctcgccattt caactagtgc agttgctaaa gtgccaacta aggttcgtct 240 tgaagcaatc ggtagtatct gcagtcaagt cgtagtcgta ggtatggcac ttgtcgtgcg 300 tctaggacgc gttgaagcta tcttattgcc cgaaggagcc gaaagtctgg gacacgtcgt 360 gggacgtatc gttaggacta tgggactagt cgtcttcggt cttacaaatt gagctactta 420 tgaaacatga atggtcggtc ttgtggtcgc ttttgtcgaa atcaccgaag tgcgtataat 480 tgatgaacga gtcgaagtcg aagttgttca acaagagagt taggtagtgc c 531 <210> 139 <211> 276 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 139 gagatttcta cgttgctgac aaagaggatg accgtgacgc aaggtctttg agtgagacac 60 atgaggctaa cggcgtccac tataccatgt ggtgtggaag cgtctttgac cgtagcgtat 120 agacgttttg caatggcgtc ttgttcattg gggctggttt cgactgtcgc tcagtctact 180 ctttcaacag tacttcgact accttcttcc atttgaccga ctttagcgaa agccataccg 240 atgaaggtag cgactttccg tcgttcccaa attagt 276 <210> 140 <211> 189 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 140 gcagatacta ccgattgctt caagacgaac cttagttggc actttagcaa ctgcactagt 60 tgaaatggcg agcacaacta ttttgaccat actgagtctg gcaaccaaga tcaccaccac 120 ataaactgga cgttctgggc taactatgag cgccacgacc aacccgacca cttaggaagt 180 tcagagaat 189 <210> 141 <211> 2462 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 141 gagacgatga aacttgagaa atcgcactgg cttacatata cacgataaaa gactttaata 60 caatataaga agtcgttatt gaagacggag atgtatgtta ccgcccaatt ttagagatac 120 aaaatgagac tgacatagcg gttaactata ttagagcacg cctaagcatt gaaggtttag 180 tcatcttcat tagagtatac tgtattgacg gctgaatgca aataaattga gacaaaaccg 240 atttcttgaa ttagcacgaa gtaagtataa aactagtaga gtgacaccaa gtatataggg 300 ggtttatatc gcgtggaatc gaacaacata tctatttaat tagtttaata acaaattaag 360 tgacttatcg cggaagagag aacaatagat tggattaaaa agttgtagta aaccacaacg 420 aagttaggta gctggtaagg ttatagatgc gttctgtcgt tgtagtccca atgaacatag 480 tatggtttcg catagtaact gacctaaaag accgccacat agtgaaaata cttaaaacgc 540 aagtttcata ggattaagtg gtagaggcta tagaaaatga tatcataact ataacgaata 600 taacagcaaa ttaaaatgca ctggaagtgg aaaatttcag caagagagtc gaagtcgtca 660 tagattagca tggataatgg caattatagt taaaatagtc gtaaattaga ttatggtgca 720 ccggggtgta agtttcgaac tcggtgaggc aatagtagta tagactaaca acaaggtaga 780 cattgcaatg tattgcgaga agaacttcac gaagatatag atacagtaca gaatttctag 840 gattaagtac agcagacgta tttacacctg ttataggata cttgttcact taatccctgt 900 tatgacaaag tagtctcata gaatttttag aacgagaaga tttgaatgga cgtaaaggaa 960 gaaggtcaaa atggagaact taaaagggga aatctagaac attgaaccca acattataca 1020 atgatataag aacgaaaaga cgaagacaat aaggtccatg ggatttaccc aaatgaggac 1080 ctaaatcacc gggaagaagt aagtaaacat aacgatatag ttttagatta tcgagaagtc 1140 gaagaagacc gggataggta acagataatc gacgtttggg aagacggtaa cgtaagaaac 1200 tgaggaggtt aaagaactaa gaatcaacaa caagaaacta ttgtccctat tctacttagg 1260 tatctgctag tatgcgatat ggaagttaaa gaggaaacag tagtatgcac tgtttgagaa 1320 aattaggatt taagaggtgg agaagtcgtt agtttggaag catagatagt tcaaatatcc 1380 acagaaggct attaaaagat agaagagagt cgagagatcg attttaaagt ctgagtcgaa 1440 atagtttggt gcaagattat ccattcaatt cttatcgaaa acttacaagg tcaacaagta 1500 gagatagcaa tataactaaa agctatctat tgaacataaa tgcgcgacta cagcttgcgg 1560 gttaacacgg tcgtcttcat tatacgttta atcgacgccg gcttattcat gcagatattt 1620 aactcaatct tcgataggat agaggacgta gtagacttgg atgtctgtca tccagacatg 1680 agacatatac ttatatggat aatggtagtg cagcaaagtc gtataattta aacggtatgg 1740 gacgaagcta acaacataga ggtggacaac gtatagcagg taattgttaa acttgttctg 1800 gaaataaaga aagaaatctt ttggactatg ggcgtggcat tagtatcaag agtagtcttg 1860 gtttgtatta tattggcagt gagcttattc gtaatagacg taatctgtcg cacttacgtt 1920 tagccaaatt aggtccgtga aacagacaca aacctatatt tatgtggcca cggacaaacg 1980 aaaactataa gctacttcgc attctgaggt cttgttgggc taataataga ctgcaatcac 2040 gttgctgggg tggtatcctt aactaacaat atggtaagta cggtgatagt gacattcaaa 2100 atagggtggt aacttagcat ggtctacttg gtggcagaag tggaagtggt agttatattc 2160 agctttatgg ttttacactg tatatttcac ttggcggtcg ccacaataaa gatttagaat 2220 ccactggcag aaagtcagcg catttttata gcagacaact tgcaaaggga gaggttcatg 2280 caacgtgtta tggtggatca agttatctta gtaagacata aacttgtata gacaataaca 2340 gtggcggaaa ttaaggttaa gataattgta actatagttc cgggagagaa ctatttaatt 2400 ccgacaactg ttatagccaa aatagatgaa atagagcgta taaaacctat tcttccaagc 2460 gt 2462 <210> 142 <211> 2247 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 142 gattggatta aaaagttgta gtaaaccaca acgaagttag gtagctggta aggttataga 60 tgcgttctgt cgttgtagtc ccaatgaaca tagtatggtt tcgcatagta actgacctaa 120 aagaccgcca catagtgaaa atacttaaaa cgcaagtttc ataggattaa gtggtagagg 180 ctatagaaaa tgatatcata actataacga atataacagc aaattaaaat gcactggaag 240 tggaaaattt cagcaagaga gtcgaagtcg tcatagatta gcatggacga aagaaaagtc 300 gcaaagatcg gaaatcaaga cgcaaaacaa aacggagaag ttcaaatcga actcgtagaa 360 catatcgctg gaacatatta aactcttggt atcgctcagt tacaaaagga gacacgtaga 420 cacgaaagaa actggaacaa cagacatatt tgtggataat ggcaattata gttaaaatag 480 tcgtaaatta gattatggtg caccggggtg taagtttcga actcggtgag gcaatagtag 540 tatagactaa caacaaggta gacattgcaa tgtattgcga gaagaacttc acgaagatat 600 agatacagta cagaatttct aggattaagt acagcagacg tatttacacc tgttatagga 660 tacttgttca cttaatccct gttatgacaa agtagtctca tagaattttt agaacgagaa 720 gatttgaatg gacgtaaagg aagaaggtca aaatggagaa cttaaaaggg gaaatctaga 780 acattgaacc caacattata caatgatata agaacgaaaa gacgaagaca ataaggtcca 840 tgggatttac ccaaatgagg acctaaatca ccgggaagaa gtaagtaaac ataacgatat 900 agttttagat tatcgagaag tcgaagaaga ccgggatagg taacagataa tcgacgtttg 960 ggaagacggt aacgtaagaa actgaggagg ttaaagaact aagaatcaac aacaagaaac 1020 tattgtccct attctactta ggtatctgct agtatgcgat atggaagtta aagaggaaac 1080 agtagtatgc actgtttgag aaaattagga tttaagaggt ggagaagtcg ttagtttgga 1140 agcatagata gttcaaatat ccacagaagg ctattaaaag atagaagaga gtcgagagat 1200 cgattttaaa gtctgagtcg aaatagtttg gtgcaagatt atccattcaa ttcttatcga 1260 aaacttacaa ggtcaacaag tagagatagc aatataacta aaagctatct attgaacata 1320 aatgcgcgac tacagcttgc gggttaacac ggtcgtcttc attatacgtt taatcgacgc 1380 cggcttattc atgcagatat ttaactcaat cttcgatagg atagaggacg tagtagactt 1440 ggatgtctgt catccagaca tgagacatat acttatatgg ataatggtag tgcagcaaag 1500 tcgtataatt taaacggtat gggacgaagc taacaacata gaggtggaca acgtatagca 1560 ggtaattgtt aaacttgttc tggaaataaa gaaagaaatc ttttggacta tgggcgtggc 1620 attagtatca agagtagtct tggtttgtat tatattggca gtgagcttat tcgtaataga 1680 cgtaatctgt cgcacttacg tttagccaaa ttaggtccgt gaaacagaca caaacctata 1740 tttatgtggc cacggacaaa cgaaaactat aagctacttc gcattctgag gtcttgttgg 1800 gctaataata gactgcaatc acgttgctgg ggtggtatcc ttaactaaca atatggtaag 1860 tacggtgata gtgacattca aaatagggtg gtaacttagc atggtctact tggtggcaga 1920 agtggaagtg gtagttatat tcagctttat ggttttacac tgtatatttc acttggcggt 1980 cgccacaata aagatttaga atccactggc agaaagtcag cgcattttta tagcagacaa 2040 cttgcaaagg gagaggttca tgcaacgtgt tatggtggat caagttatct tagtaagaca 2100 taaacttgta tagacaataa cagtggcgga aattaaggtt aagataattg taactatagt 2160 tccgggagag aactatttaa ttccgacaac tgttatagcc aaaatagatg aaatagagcg 2220 tataaaacct attcttccaa gcgtaat 2247 <210> 143 <211> 2498 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 143 gatgaaactt gagaaatcgc actggcttac atatacacga taaaagactt taatacaata 60 taagaagtcg ttattgaaga cggagatgta tgttaccgcc caattttaga gatacaaaat 120 gagactgaca tagcggttaa ctatattaga gcacgcctaa gcattgaagg tttagtcatc 180 ttcattagag tatactgtat tgacggctga atgcaaataa attgagacaa aaccgatttc 240 ttgaattagc acgaagtaag tataaaacta gtagagtgac accaagtata tagggggttt 300 atatcgcgtg gaatcgaaca acatatctat ttaattagtt taataacaaa ttaagtgact 360 tatcgcggaa gagagaacaa tagattggat taaaaagttg tagtaaacca caacgaagtt 420 aggtagctgg taaggttata gatgcgttct gtcgttgtag tcccaatgaa catagtatgg 480 tttcgcatag taactgacct aaaagaccgc cacatagtga aaatacttaa aacgcaagtt 540 tcataggatt aagtggtaga ggctatagaa aatgatatca taactataac gaatataaca 600 gcaaattaaa atgcactgga tgttagagca aattatataa actttcacat gctaaccaac 660 gtaatttgag aagaaatcat caagaattaa actaaaggaa tggtggataa tggcaattat 720 agttaaaata gtcgtaaatt agattatggt gcaccggggt gtaagtttcg aactcggtga 780 ggcaatagta gtatagacta acaacaaggt agacattgca atgtattgcg agaagaactt 840 cacgaagata tagatacagt acagaatttc taggattaag tacagcagac gtatttacac 900 ctgttatagg atacttgttc acttaatccc tgttatgaca aagtagtctc atagaatttt 960 tagaacgaga agatttgaat ggacgtaaag gaagaaggtc aaaatggaga acttaaaagg 1020 ggaaatctag aacattgaac ccaacattat acaatgatat aagaacgaaa agacgaagac 1080 aataaggtcc atgggattta cccaaatgag gacctaaatc accgggaaga agtaagtaaa 1140 cataacgata tagttttaga ttatcgagaa gtcgaagaag accgggatag gtaacagata 1200 atcgacgttt gggaagacgg taacgtaaga aactgaggag gttaaagaac taagaatcaa 1260 caacaagaaa ctattgtccc tattctactt aggtatctgc tagtatgcga tatggaagtt 1320 aaagaggaaa cagtagtatg cactgtttga gaaaattagg atttaagagg tggagaagtc 1380 gttagtttgg aagcatagat agttcaaata tccacagaag gctattaaaa gatagaagag 1440 agtcgagaga tcgattttaa agtctgagtc gaaatagttt ggtgcaagat tatccattca 1500 attcttatcg aaaacttaca aggtcaacaa gtagagatag caatataact aaaagctatc 1560 tattgaacat aaatgcgcga ctacagcttg cgggttaaca cggtcgtctt cattatacgt 1620 ttaatcgacg ccggcttatt catgcagata tttaactcaa tcttcgatag gatagaggac 1680 gtagtagact tggatgtctg tcatccagac atgagacata tacttatatg gataatggta 1740 gtgcagcaaa gtcgtataat ttaaacggta tgggacgaag ctaacaacat agaggtggac 1800 aacgtatagc aggtaattgt taaacttgtt ctggaaataa agaaagaaat cttttggact 1860 atgggcgtgg cattagtatc aagagtagtc ttggtttgta ttatattggc agtgagctta 1920 ttcgtaatag acgtaatctg tcgcacttac gtttagccaa attaggtccg tgaaacagac 1980 acaaacctat atttatgtgg ccacggacaa acgaaaacta taagctactt cgcattctga 2040 ggtcttgttg ggctaataat agactgcaat cacgttgctg gggtggtatc cttaactaac 2100 aatatggtaa gtacggtgat agtgacattc aaaatagggt ggtaacttag catggtctac 2160 ttggtggcag aagtggaagt ggtagttata ttcagcttta tggttttaca ctgtatattt 2220 cacttggcgg tcgccacaat aaagatttag aatccactgg cagaaagtca gcgcattttt 2280 atagcagaca acttgcaaag ggagaggttc atgcaacgtg ttatggtgga tcaagttatc 2340 ttagtaagac ataaacttgt atagacaata acagtggcgg aaattaaggt taagataatt 2400 gtaactatag ttccgggaga gaactattta attccgacaa ctgttatagc caaaatagat 2460 gaaatagagc gtataaaacc tattcttcca agcgtaat 2498 <210> 144 <211> 428 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 144 ggctatagaa aatgatatca taactataac gaatataaca gcaaattaaa atgcactgga 60 taataaaaat taaactaata tttgtagtta gtggtgcaga tttgaacgaa cagtcaggcg 120 aaatggaagt aatagatgat tcatggacga taagtcatta agacatcgaa gactgttttg 180 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 240 aaaaatccta agagatcgac gaagttatcg actaaattgt tgtaatccaa gaggctgatt 300 gacgtttaga agtaagtggc aatttggttt aaaacaacga taataaaggt tgcgaagaaa 360 tataaaaagt agtcacataa cactgacatt attaaattta gatagacttt accgctaaaa 420 ctgtagtt 428 <210> 145 <211> 2462 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 145 gtgagacgat gaaacttgag aaatcgcact ggcttacata tacacgataa aagactttaa 60 tacaatataa gaagtcgtta ttgaagacgg agatgtatgt taccgcccaa ttttagagat 120 acaaaatgag actgacatag cggttaacta tattagagca cgcctaagca ttgaaggttt 180 agtcatcttc attagagtat actgtattga cggctgaatg caaataaatt gagacaaaac 240 cgatttcttg aattagcacg aagtaagtat aaaactagta gagtgacacc aagtatatag 300 ggggtttata tcgcgtggaa tcgaacaaca tatctattta attagtttaa taacaaatta 360 agtgacttat cgcggaagag agaacaatag attggattaa aaagttgtag taaaccacaa 420 cgaagttagg tagctggtaa ggttatagat gcgttctgtc gttgtagtcc caatgaacat 480 agtatggttt cgcatagtaa ctgacctaaa agaccgccac atagtgaaaa tacttaaaac 540 gcaagtttca taggattaag tggtagaggc tatagaaaat gatatcataa ctataacgaa 600 tataacagca aattaaaatg cactggaagt ggaaaatttc agcaagagag tcgaagtcgt 660 catagattag catggataat ggcaattata gttaaaatag tcgtaaatta gattatggtg 720 caccggggtg taagtttcga actcggtgag gcaatagtag tatagactaa caacaaggta 780 gacattgcaa tgtattgcga gaagaacttc acgaagatat agatacagta cagaatttct 840 aggattaagt acagcagacg tatttacacc tgttatagga tacttgttca cttaatccct 900 gttatgacaa agtagtctca tagaattttt agaacgagaa gatttgaatg gacgtaaagg 960 aagaaggtca aaatggagaa cttaaaaggg gaaatctaga acattgaacc caacattata 1020 caatgatata agaacgaaaa gacgaagaca ataaggtcca tgggatttac ccaaatgagg 1080 acctaaatca ccgggaagaa gtaagtaaac ataacgatat agttttagat tatcgagaag 1140 tcgaagaaga ccgggatagg taacagataa tcgacgtttg ggaagacggt aacgtaagaa 1200 actgaggagg ttaaagaact aagaatcaac aacaagaaac tattgtccct attctactta 1260 ggtatctgct agtatgcgat atggaagtta aagaggaaac agtagtatgc actgtttgag 1320 aaaattagga tttaagaggt ggagaagtcg ttagtttgga agcatagata gttcaaatat 1380 ccacagaagg ctattaaaag atagaagaga gtcgagagat cgattttaaa gtctgagtcg 1440 aaatagtttg gtgcaagatt atccattcaa ttcttatcga aaacttacaa ggtcaacaag 1500 tagagatagc aatataacta aaagctatct attgaacata aatgcgcgac tacagcttgc 1560 gggttaacac ggtcgtcttc attatacgtt taatcgacgc cggcttattc atgcagatat 1620 ttaactcaat cttcgatagg atagaggacg tagtagactt ggatgtctgt catccagaca 1680 tgagacatat acttatatgg ataatggtag tgcagcaaag tcgtataatt taaacggtat 1740 gggacgaagc taacaacata gaggtggaca acgtatagca ggtaattgtt aaacttgttc 1800 tggaaataaa gaaagaaatc ttttggacta tgggcgtggc attagtatca agagtagtct 1860 tggtttgtat tatattggca gtgagcttat tcgtaataga cgtaatctgt cgcacttacg 1920 tttagccaaa ttaggtccgt gaaacagaca caaacctata tttatgtggc cacggacaaa 1980 cgaaaactat aagctacttc gcattctgag gtcttgttgg gctaataata gactgcaatc 2040 acgttgctgg ggtggtatcc ttaactaaca atatggtaag tacggtgata gtgacattca 2100 aaatagggtg gtaacttagc atggtctact tggtggcaga agtggaagtg gtagttatat 2160 tcagctttat ggttttacac tgtatatttc acttggcggt cgccacaata aagatttaga 2220 atccactggc agaaagtcag cgcattttta tagcagacaa cttgcaaagg gagaggttca 2280 tgcaacgtgt tatggtggat caagttatct tagtaagaca taaacttgta tagacaataa 2340 cagtggcgga aattaaggtt aagataattg taactatagt tccgggagag aactatttaa 2400 ttccgacaac tgttatagcc aaaatagatg aaatagagcg tataaaacct attcttccaa 2460 gc 2462 <210> 146 <211> 949 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 146 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtgg aatcgaacaa 360 catatctatt taattagttt aataacaaat taagtgactt atcgcggaag agagaacaat 420 agattggatt aaaaagttgt agtaaaccac aacgaagtta ggtagctggt aaggttatag 480 atgcgttctg tcgttgtagt cccaatgaac atagtatggt ttcgcatagt aactgaccta 540 aaagaccgcc acatagtgaa aatacttaaa acgcaagttt cataggatta agtggtagag 600 gctatagaaa atgatatcat aactataacg aatataacag caaattaaaa tgcactggat 660 aataaaaatt aaactaatat ttgtagttag tggtgcagat ttgaacgaac agtcaggcga 720 aatggaagta atagatgatt catggacgat aagtcattaa gacatcgaag actgttttgg 780 atgtagacat atttgtagaa cattaaagaa agatatgagt tctttatttc aaaaacatag 840 atggttggct ggttagcaac gtcaaagccc acagtatcta atgttgttat ggcagaaaca 900 gacaaaatat caggctacaa aaatgtatca gagcattaac atgaaaaat 949 <210> 147 <211> 2326 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 147 gcactggctt acatatacac gataaaagac tttaatacaa tataagaagt cgttattgaa 60 gacggagatg tatgttaccg cccaatttta gagatacaaa atgagactga catagcggtt 120 aactatatta gagcacgcct aagcattgaa ggtttagtca tcttcattag agtatactgt 180 attgacggct gaatgcaaat aaattgagac aaaaccgatt tcttgaatta gcacgaagta 240 agtataaaac tagtagagtg acaccaagta tatagggggt ttatatcgcg tggataataa 300 aaattaaact aatatttgta gttagtggtg cagatttgaa cgaacagtca ggcgaaatgg 360 aagtaataga tgattcatgg acgataagtc attaagacat cgaagactgt tttggaagtg 420 gaaaatttca gcaagagagt cgaagtcgtc atagattagc atggatttcg aagaacgcga 480 agaactaaac gaacaagtta tgaacgaaat tagagtccta ataacctgtt tagtgaattg 540 accagtagtc atataacaaa aacaagaagg ttaggcaaag ctataagcaa atagcattga 600 aatagaaatg gacgagaaga aggctttaaa atggtagtcg tatgcgaacg ctaaggaaga 660 gaaattctag tagaactcgt agttacatac ttggacgaaa gaaaagtcgc aaagatcgga 720 aatcaagacg caaaacaaaa cggagaagtt caaatcgaac tcgtagaaca tatcgctgga 780 acatattaaa ctcttggtat cgctcagtta caaaaggaga cacgtagaca cgaaagaaac 840 tggaacaaca gacatatttg tggatgttag agcaaattat ataaactttc acatgctaac 900 caacgtaatt tgagaagaaa tcatcaagaa ttaaactaaa ggaatggtgg ataatggcaa 960 ttatagttaa aatagtcgta aattagatta tggtgcaccg gggtgtaagt ttcgaactcg 1020 gtgaggcaat agtagtatag actaacaaca aggtagacat tgcaatgtat tgcgagaaga 1080 acttcacgaa gatatagata cagtacagaa tttctaggat taagtacagc agacgtattt 1140 acacctgtta taggatactt gttcacttaa tccctgttat gacaaagtag tctcatagaa 1200 tttttagaac gagaagattt gaatggacgt aaaggaagaa ggtcaaaatg gagaacttaa 1260 aaggggaaat ctagaacatt gaacccaaca ttatacaatg atataagaac gaaaagacga 1320 agacaataag gtccatggga tttacccaaa tgaggaccta aatcaccggg aagaagtaag 1380 taaacataac gatatagttt tagattatcg agaagtcgaa gaagaccggg ataggtaaca 1440 gataatcgac gtttgggaag acggtaacgt aagaaactga ggaggttaaa gaactaagaa 1500 tcaacaacaa gaaactattg tccctattct acttaggtat ctgctagtat gcgatatgga 1560 agttaaagag gaaacagtag tatgcactgt ttgagaaaat taggatttaa gaggtggaga 1620 agtcgttagt ttggaagcat agatagttca aatatccaca gaaggctatt aaaagataga 1680 agagagtcga gagatcgatt ttaaagtctg agtcgaaata gtttggtgca agattatcca 1740 ttcaattctt atcgaaaact tacaaggtca acaagtagag atagcaatat aactaaaagc 1800 tatctattga acataaatgc gcgactacag cttgcgggtt aacacggtcg tcttcattat 1860 acgtttaatc gacgccggct tattcatgca gatatttaac tcaatcttcg ataggataga 1920 ggacgtagta gacttggatg tctgtcatcc agacatgaga catatactta tatggataat 1980 ggtagtgcag caaagtcgta taatttaaac ggtatgggac gaagctaaca acatagaggt 2040 ggacaacgta tagcaggtaa ttgttaaact tgttctggaa ataaagaaag aaatcttttg 2100 gactatgggc gtggcattag tatcaagagt agtcttggtt tgtattatat tggcagtgag 2160 cttattcgta atagacgtaa tctgtcgcac ttacgtttag ccaaattagg tccgtgaaac 2220 agacacaaac ctatatttat gtggccacgg acaaacgaaa actataagct acttcgcatt 2280 ctgaggtctt gttgggctaa taatagactg caatcacgtt gctggg 2326 <210> 148 <211> 889 <212> DNA <213> Artificial Sequence <220> <223> transcript variant <400> 148 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtgt ttaataacaa 360 attaagtgac ttatcgcgga agagagaaca atagattgga ttaaaaagtt gtagtaaacc 420 acaacgaagt taggtagctg gtaaggttat agatgcgttc tgtcgttgta gtcccaatga 480 acatagtatg gtttcgcata gtaactgacc taaaagaccg ccacatagtg aaaatactta 540 aaacgcaagt ttcataggat taagtggtag aggctataga aaatgatatc ataactataa 600 cgaatataac agcaaattaa aatgataata aaaattaaac taatatttgt agttagtgtc 660 aggcgaaatg gaagtaatag atgattcatg gacgataagt cattaagaca tcgaagactg 720 ttttggatgt agacatattt gtagaacatt aaagaaagat atgagttctt tatttcaaaa 780 acatagatgg ttggctggtt agcaacgtca aagcccacag tatctaatgt tgttatggca 840 gaaacagaca aaatatcagg ctacaaaaat gtatcagagc attaacatg 889 <210> 149 <211> 10643 <212> DNA <213> Artificial Sequence <220> <223> SIRV1-GENE <220> <221> variation <222> 241 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 149 gtgaggcatc tataccgcca cgccctatgt ctttctcacg tataggtgtg ctctcagctg 60 ccgttccggc gctgcttcac ccctttccgc ttatcgcgga tggtaaagtc aaagtttact 120 cagcggtcgc tgttgacctc aggttctcta tcaaaaattt gctggctaaa acggtcggag 180 cttctgctcc actctttcca gcaatccttc tgccagtcgc cttcaaactt gcaatctgtg 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattcgtcg aacgtccggg 420 tagtatagtt atctgcatcg tggtcaccag tatcacagac tacaaaatct caatcctcca 480 ttcccccgtc aggtctatct aaaattcaat cgctgtgttt gcggggtaaa taataggaca 540 agtccgtata atctacccaa tcgcattcat tccagtcaga gggtaccacc gaatttcatc 600 aacaacatta ataattccat aggaactaca ttcttaaatc cttggaactc caaactccta 660 ccttatcacg gttctatccg tcgtcttagg gtcggtctcc tcgtagcatg gcctcgttct 720 cctcgccgta attgcggtcg aaagtacttc agttatttat gnnnnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nngtgaggct tgtccgcgag tgtcaaccgg gttttacaaa ggagaacttc 900 caacatcagt gtatgggatg tttcaaacac ccccagagac ggagacaata gtgtggtcac 960 gtacatttag gaagataaaa ataacaacaa cagaaggtgt gaggcgcaag gtacatgctc 1020 ttaccccact ctcagcacgt agggagcgga gtgtggaaga ccaacaagcc cgtcgcctct 1080 accaaataca gctatcctga ggtgccaggt tagaataggc cctaccacat ctaagccttg 1140 aaacggtctg ttcctcgtgt caagttcttt gtccgcttat accgccatct ctcgttgtgn 1200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnngta caagagttgg ttactctcag tcccattccc aacacatcac 1320 tgagaagtat agagcctagt ggtcattaga aggtcaacat gnnnnnnnnn nnnnnnnnnn 1380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agtaggtgtt 3840 taggtgtgtc ggagggcaga aaccaacgaa agaaaggaaa cgatcaacac ttatatagtg 3900 tcctgtcggg cccaaatcct aagagacctt aggtttacca cattagtact aaacagcctg 3960 ttgtataccg tgagccccca cttcgatctg taaatggtaa tcccttgtaa aacctcatct 4020 ccaaaaacag tatacgcagg tacagctgat gtgatcctct ctactagtct tctctgagga 4080 accatgagat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatagtgggg gacctgagtc atactagggt 4860 aacttcttat atggtgacat cactcctacc gtagaggggg gacggtagtc tgtgggaaat 4920 ctaccctagt gaagaaaggt aagagaatgt ctgaacatta cctgtagacg aaggtactct 4980 cagaacaact actctcacat acggaaaggt ttgggtcctt ggtcaaagga ctttactgac 5040 tctgcagacg atgaatatct gaaccaacac tactatcaaa gatgtcgtag gtttgtgaga 5100 agggagaaga agtggttgag aagttggtgt tccacaagtt aaagtagaca tacctttagt 5160 acctgtaaag gttcgactag acctctcgtg gtagggcctg gaagtcaaaa gacttctcta 5220 tgggtactag gtaggtagtt agagtacggt ccctggggtg tctcagagtc gaacgttcgg 5280 gggaagttcc taaacatgtt aggatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10140 nnnnnnnnnn nnnnnnnnnt tatgataaca agtccaaacg tggacgtcgt cgctgtcacc 10200 tctcggaaca ctagctgagg tggcttaaaa acattccgta actgagtggt caccgggggt 10260 gaaacatgct gagggttcta ccttaacggg aaggtgaaac tctccaatgt ctttgaaagg 10320 actagttgag ataacccaaa ccgaatccgg aggaagagga gaacttggaa aacgttgtac 10380 gacgtcatta ataacgattc cgttcttccc gttcgtgtct ataaggatat tctttcattg 10440 cgaaggtaag cccttttggg tctagatacg gactatactc aaaaacccac agaccttcca 10500 cttggagttg ttttaggaga cggagtagta gagaaaactt gtggcacctt cccagtcggt 10560 tccctacaga ctctccttag tagctcctaa gatcgtcgag ttacacgacg acattgttcc 10620 gactgacgta gaggcattac ttt 10643 <210> 150 <211> 4911 <212> DNA <213> Artificial Sequence <220> <223> SIRV2-GENE <220> <221> variation <222> 161 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 150 gttggtgtat ttatccgcta cgtggtggat gaacatggat gcgaacgaca tttcgaaata 60 tacgcaatga tatccaaccc taccgaaaca gcaataattg tagaaggtag catatcatgc 120 cgagtcatcg acatgccttt atcgacacac gacgtgattg nnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagtttact acgttgtagc gagtttagtt 1140 gaaatagttt gtgatgtgta aactaaaaca taccaaatgg taaaacttgt cacccccatt 1200 tacattgcga cagacacagt tatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagtggat 1440 ggaagtgtgt tcgactgtca agacccttct ataatagtcg aaccagtacg gtaagtgggc 1500 aacatgccca tataagtcga agcatttcaa atggttcatc cagtagacga cgatgtccta 1560 gacagtcatg tctgtcacat ctatcacgcc tcctacgttg tgccataacg aagaataata 1620 gtgataagag acatcgaagc tgtcgtagtc gttcgctaaa ttgcttacaa tttagtgctc 1680 taagtgaaca atgtttcatc ttgagaagca aacgaagtag aagttgacga cgcattcgaa 1740 gacgtggaca atgtgacacg agtatcgaaa catgatccaa cgaacatcga agaagttact 1800 actgaactac gggtcagctt ccccatttga agtcatgtag tggttgtcta ctttttcatt 1860 gaagacgtcc acctcaacgc tatgaacgtt agttgagaca atgttaaagc aaacgacaac 1920 gtcattgtga tctacataca cagtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatg gttagcgtaa 2100 acatggtgga acgtactttg actgcgctgc aagaaatggt tgggtcgatc gtaatgctag 2160 tcgttacatc ggaacaagcc aaaacaaatc attcgctgga tttagaccta ctgcacgacg 2220 acgtcgacac aagacattct tgaaaggtaa ttgacgtgga cgtttcagnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnngaa ttcaatgcag aaactcctat gcacgtcgaa ctagtctctt 2580 gctcagcgct agtccatgaa aatgtcctag ctttgaacgt ttatcaaatg caagttgtgg 2640 gagttagtca tgtgagagtt gtgcaagaaa gtgttaacgt tctagccgtg ctcagtaaca 2700 attaccattc ggcggtaaca aacgtaagtt gtcactgcac tcatgagcat gaagttagac 2760 cagtagttgg gtcagaaatt agacgtggtt tagtctggtt accgtgnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn ngaaagaata gctgaactca aagaagccga caaagataaa 2940 caaagaagaa acggttaata cgattgagga gaaatattca atccaacggc aactattaac 3000 gtttcaacta gaatctacgt ttcatacgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attccacatc 3120 cgttcttgca attaaagaaa cgatgtagaa aacgctttgg ttggcgacaa cttgggcggc 3180 aaaataatta tggtaggtaa ttgaagtagc gttaaaacgc aaaaagaagg aagcacgnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnngat cactgcaaaa aggactactc aataatggtt tctaaagtca ttatgctggt 3900 gctccctatg gcggtggtca gtactgtaac taaagatgnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttacggcact tgtagagcac 4080 gtatcagacc cagctttgaa agaaggctga gttcggttcg ggtttcacgt aggcctagaa 4140 cgaggtatag aagtggttaa caaagatttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttaccatgt 4260 aagaagcggt agacgccatg gcctagtagg aagaatgctt gttcacggag ccgacgaagt 4320 tagacgagtg gcagaagaaa gagcattatc ttggtactcg gactgaatgc gtactacggt 4380 cgtccaagtt gcaagaggta ttagagtcat tcgaatagct gtgggagtgg tatttatagc 4440 ataataagtg taaaaaacat tcccaacagc atatgtgtag ccaagaagcg ttgtgctcgc 4500 gactaaaagt caggtagcag caggcattaa cccagtgata gaacatcaaa gtcaaataag 4560 tatggtttag cattactgcg tatcttgcga ttgcgtgctg gtggtcaaca gtgcaaacct 4620 agtggcttcg cactcaaatg tagacaagca ttaaccaact acttctaacg tatgttcagg 4680 aagaatggct attctcgtca ggtgaatgcg taatcgaatg agtagtatgg tcgaacgtcc 4740 cgctatagtc tgacacgatt caggcaaagt tggagtgcaa agccatgatt tggtcgatgc 4800 ggcaaatgtt gcttaggact attctatggt cttgcgtaat tgtgaagatt tgccagttat 4860 ctaaggagac gtggtgattc gctctatagc tgtatctaat ctgccagatt g 4911 <210> 151 <211> 8943 <212> DNA <213> Artificial Sequence <220> <223> SIRV3-GENE <220> <221> variation <222> 168 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 151 atgacgagaa gcatttcaag aaagcgttgt agcttaaaaa gaacgttgcg gtgctaaagg 60 agaaagtcga aaaagcgaag gagttaaaac aaaagccggt tattaaatgc tggaagaagg 120 aagaacaaca actaaacaag gttgtcaaga gcattaagga agccgtannn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtttcaac 540 aatgcccaag ttgacattat tccaaaacgt ttcgtagaaa tgaaagcgct tcgagtaatt 600 aggcgcaaac ttacaggacg ccatggcata tgggtggtag aaccaacgat ggaagcgagt 660 agcgcaatgc cttagcgcac gcctacacta actgcttacg aagttggagc aaaagaaact 720 gaacgtgtca gccaaaattg ccaagaaaca ggagctacca aaagnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nntggccaaa caacactcgc aaagaattta tgtgcgcttg gcaggtggag aagcaaatac 960 ttggataccg aatttagagg ccataggtta tggaaaaagt cagtgnnnnn nnnnnnnnnn 1020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnngtacgaa ttacgaacac cttaacatta gggaaactta gatagttgta caaagtaacg 3060 tataacaagt atcgcgtatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttagatagta aaagtcgaaa tctttatatt 3600 cggttcgatg tactacgtta ttcgcgactg ttctgtcatg atagtttttt atatagtcct 3660 aataaaagga gtttttaaag taaatgacat ttcagtagta aaagtttaag agctttttgt 3720 tctgccagac gttctgatag ttgaggaaaa cgtgtggtca gctaataact tgtggtatgn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5040 nnnnnnnnnn nnnnnnngag taaacgaaca tccaacagta tgtacaatcg tatgagaaag 5100 gacaaatctg aagacgatat tagagggagt ccaacaagtt tgtgatgatt cacgtaagga 5160 aatcgaaatg taagattatt cgtcaatgga ggtataagac aacacaccaa gtaattatac 5220 gcgacgagtt ttcatgttga cgatccctga gactatatca tacaaaattg acccaaataa 5280 taaactcgtt ccaatagttg aagtgttgat taacacattg agacaagttg tccgtacttt 5340 aatcatcaaa gttttggact gacctataaa tgaaatattc gcgttgtctt cagtttaaga 5400 agtgatatta ttttcggttt caagcctatg tggagagata caggaaatag gtaaactata 5460 actaatataa atatacgcag acgaagcctg ttaactttac caaatccaaa tagcgggaga 5520 cactaaactt acaagaaatg tacggtaaga cagggaaaca gggtttgaag tttggaggta 5580 caaaatctta atccttcaca actgaatcaa catttacatt cggttacgtt cggggtttgt 5640 agtagtaatt gttgaggtaa ttttaattgc acttacagac gtggatttgg tgaaacatta 5700 tgtggtagat tcatatttcc gaatcttaat agacgatttt atgatggtga tcgtggtttc 5760 ttttcaaggt ttggtggacg aaaatgaaaa aatcacaatt taacgtttca tattcatcac 5820 aatccgaaat cgagatggag agaaagtttg actaaacttt gttacagtat ttttgaaggc 5880 aatcaacctg gaactttgga tttcatcaag taaacaccca tatttagcga aaatgcaagc 5940 taactgcgaa ctaacaaggt aactcttttt cgatttcaaa atatggtcag tagcaatatg 6000 ttaagtgatc cattttggag gggtggggat ttgttcaatg aatgaggcaa taaaccaccg 6060 caacctctgt aattgacgag aaacttgtat aaggtcatca aaaagaatag atgaacataa 6120 agattgaata cggctgggtg aggttaattc agatttccca aattatgtag atgaaaagat 6180 ggtaagttta ttgataggta aaaaatcaag agagggtaat aaagaagaaa gaataatcaa 6240 aagatagagc atgcaaacat tacatggtag tattgttata ggcggtatat tggtaaatga 6300 cgatccttga cttagttttt tacatcgaac ggttgcaaat cctagcatac ttgatattaa 6360 tagtagatac tgaaatatat ctgagtatat caagggaaat cgagatgtaa caataagtcg 6420 aggaaattaa ggtcaattta gtgcttatca tagaaataac tacttaagcc cttaacgaac 6480 taactcaaat taacgaggta gaggttattc tgaattgtac gaacgtagtc gaagtttgtt 6540 accaagggta aagccaaaac aaaacgattt gacaagagaa tctattccat gtagtagtgc 6600 gtttgtacga cgttctattc gatgtaaatc attaggacga gggtttgaag caactcggaa 6660 agatggtcgg ttagaattta aaagtactga acggaattaa cgaagttggc tactacctaa 6720 tagtctttgc ttaggattta gtagttcgca attgggtcaa agtggtcgct ttggtcgtcg 6780 acgtcaagac tcaacaagtc atttaccgct gtacgaacca tctcatttaa caggttctca 6840 accaaatagc ggtaggagta gaaagcactt accatcgtac gaactttgac aacgaaaggt 6900 tcaaaggtaa ccaaggcgta gtagtgagtt aggtcaatgt ataaaaactc cctttcttat 6960 tttgagtcgt caatgtagtc gagaaagtgt ccaagcaaaa cgtaacgaaa cggttgagta 7020 aggacttgca cactatgccc ttgcgggttt acctgcttga cgtagtcctg ccacttatta 7080 tccgtgattg ttacctaatg gacaacctaa tcgttagctt aaagaagatg cacttagtgc 7140 cattattatt agacaagata gtccgttctt tagtcgaagt tcttgttgta ataacaaaag 7200 tagagcagta ttttgacgta tagggagaca gtcattaggt cattaaccca gttactaagg 7260 acaacgtaac catcgaagtt attgaacttc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7740 nnnnnnnnnn nnnnngatta cgatccgaaa tttcatggac acagatattt gagatcaaca 7800 tttgattcgt tgacgtattt atgttgattc ggttagcgag agttacaaag ttagtaatag 7860 aaagaaagag gaaataataa tcaaaagatt aagcatgcaa acattaagag gacacatatg 7920 tattggtggc atttgcgtta ggttaacgtc tcgtaagacg gtgaagctgc ttaaannnnn 7980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggttaggtc gcttggtagc atatctgtta 8220 ccgaagacac gacagtaaat tgtcaagcaa acactgcgcc ttcttcggtc gcataaagaa 8280 gtgaaccata acgttctcct acttaactcc gcaacgaaaa agagnnnnnn nnnnnnnnnn 8340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncattcg ttcaacatga agtggcttat 8460 gttcgcggag tagtagcgcg taaaagtgta ggtacgcgag ctatcgtgcg gtgtagcatc 8520 gcagtcaaag cttgcgtagg tagcgttgca atctcctgta ctaaagacat gcaactgcca 8580 gcttgtactg ttggcgaaga aagttcagtg gatgttacta accgcgttta acgcgtggac 8640 acggcggcag ttgagccaat cgattacgaa acaagctatg ctaatgttga ccatttggct 8700 cttcgaccag cagtcagccg cttctttacc tgccaacgtt gttcataaca ggcaaaacca 8760 gaaagtcgca taggttggtc aactttcgca gaaccaactg ttactgcgat cttcagcttg 8820 ccttttgtcc cagattaacg tgtaattatt aaacaaagtt cggcagttcc agtcgctatc 8880 gacaaaaccg aaaccacaag ttctgtaatt acgcgttcgg caaagcgcaa tgcgccctaa 8940 tac 8943 <210> 152 <211> 14122 <212> DNA <213> Artificial Sequence <220> <223> SIRV4-GENE <220> <221> variation <222> 204 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 152 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggaatacaga acgataacgc 120 agcagaagta gttgaccacc gtgtaacttg aatggtgatg gtcctggttc tattaataca 180 aatcctttta cttatgctcc gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnng caattaggat caggtggtta atctgatacg nnnnnnnnnn 540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccaa ggtgaaccag 1200 gacgaagtcg atgtttcaga acctccttta ggccttttag acggtttaaa cgaagcacta 1260 gaccaagtaa taaaagcgga cgttgcacag gtacgtgcag atacagaaca actaaaagaa 1320 ctaaaagaga agaacgacgt gcacgtgcta attatgcgaa ttaacatcgg gttagaaaaa 1380 gtaaactaaa ggaaaatggg taaggcatat gccttactaa attgtgtaaa agaagttcta 1440 ataagaaccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6120 nnnnnnnnnn nngaatgtat gtaattattc gcaagggttc cataaggcac atatacaaat 6180 ttagaaacaa cccgttacat taacaaaggt gaacggtgtt tcacagatat tagaccatta 6240 ggtaaccaat gacatttcac tatcgaaaag ggtatagtat taactactac aactaatccg 6300 actcgtcgag gtgatttata caggactgca ggtagcaaac gacgaggtgg acgagggtaa 6360 ggtggccgac gaaagggagg acatgcacct agttgaacac gactaggagg acgatggcca 6420 ctacggagtg gaacacaaag gtaaagttgc aataataacc agtacaacaa aagcatacgt 6480 ggaaggtgac aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caaacgatgt cttgaacaac accgtcttca 6780 ttgtttacca tttgctgttg gatttgatat ttagagcgta tgttgaagaa gttggaactc 6840 tggacacatt agagacttat tggatcgatc aagaggaatg nnnnnnnnnn nnnnnnnnnn 6900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7200 nnnncaatac gaagtagttt cactttcgta ggttacccta taacacgcaa atannnnnnn 7260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7440 nnnnnnnnnn nnnngatgta atcaacctct tattaatcat annnnnnnnn nnnnnnnnnn 7500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgtaac catcttacgg 9300 atgtaatcta acgtaaacac cagagaaaca accatcaaaa cggcataatg ggcaagcatt 9360 attaggaaca tctaaatata aaaccattgc cttaactcac ataacgctac acttggtaac 9420 accaccatat gcagtgcaag aacgttgggt aagagaacat cgaacgtatg gtctgtttac 9480 ataagctcaa aaataatttt acagggtaaa acgtcaatca ttacttaaca gcagtaatgc 9540 ttacaaatgt ttcggttagt atttaaaacc gtaacgaacg ccacgtgtaa aaacaagagg 9600 ttttattaat attgggtata ttagatgtat ccgtcgatta acccaccata accaaactaa 9660 attaaagcaa cgacttatgc gaaatcctcc ttgtttacaa actaacagta atgcaagtcg 9720 taataaacgt cttaaatatt ttataggtaa aagatttgga ccatatcctc agtactgtaa 9780 tgataaacac cgacgaagtc tacatcgttg caacggaaaa ttccatgaaa gtcgtatgtt 9840 ttatggtaaa cttccgaatt tagcaaaaag ggttgtaaaa ggtatacgtc atcatggtaa 9900 gaaatacagc tgtattagta tataaggttg tagaaaacca cataataata tgtctttagc 9960 actagacatc agatgttaat gacctaacga ttatagtgct gaacaacgtc tacgacaata 10020 taaacctttg caaagactgg tcaaacatca atgttaaaca cgtccagata aaagcaaaaa 10080 ggtaagacga ggattacgga ataattaata tagtgnnnnn nnnnnnnnnn nnnnnnnnnn 10140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttgttccaat cggcccggct 11040 tcagtcattc ccggtggcga agccatgtca ccaataaaaa ctatcggcgg caatggaagt 11100 ggtttttcgg aaactgttac gggtgcaagc ggccccagtg cagtttgtgc cttgccaatc 11160 ggcccagctt tcaaacgcaa agttggacga cttattggcc cattctgaca acgtcctgtt 11220 aaactgcccg gacgttcaaa cggcggtatc ggacgactaa cgcgttacgg cggacaactc 11280 gcccccagtc aacaacttgg acacggtaaa tttgaacgct aagtcaactg tagcagttaa 11340 acattgagta ttaaaataga tttaagctag cgttaagaac attaaaaact aaccagccaa 11400 accaaggatt acggctgtgg tgtaatcgat tgcgaaatag catgacaaaa aacttacagt 11460 ttagaaggtg gcggtattac ttaagaacat ttaaaaatag cctgttaact tcaagctgta 11520 gtagcctaaa caggtttcct aatagtatgc aaacaacata gttcaataga agttattaat 11580 aaacatcaaa ttgtctttga acaacacaaa cacgtaagct ttcggcaact aaattaacta 11640 aaaaataact ataattacac agaacacgaa gtcatctgtt tcctattaaa aaataggtac 11700 ttgacaggtt acaatataac ctgtttctag catgtataac aaaattaagc gacgaccgag 11760 cgacgacaac aagcagcagc caggtgggca aaaggctaag actgctttga tgtcctgagc 11820 caacttgccg atacgcagca acattttaga aacgtcatcc tgaccgccgc cgccattgcc 11880 ataaatgata gcttggcggt agccgcccaa aactatgaaa aaaattaaat taaggaaaga 11940 cataaacaag gtagttaagc catgcaccta gaaaattttg aacggctcag ctgcaacata 12000 ttagcgctag aaatgaccta ccataatgat atagaagata attaccatta ctgccaccgc 12060 ctcctccgct gccgcctcct ccatagcagc ttctattcaa acaaactccg ccgccgccac 12120 cgccgccata accaccacca taaccaccgc cgtatacaca aacgccgctc cttctaagtc 12180 ttagctatta ataacaaccg tggaaacaaa aaacgtaata taggctacag ctgtgtcaac 12240 agccaaatcc ataacaacaa aatccatggt gactgtaaca gacactgtta caaccatatt 12300 attaactaga tagtggttac agataatcat gcagcaacaa catataaaga agacggttaa 12360 agttattgac ttatgttagc atgtgcaaat taacataggc tagtcttaac ctgactcgct 12420 gtcgcgactg gcatgcaaag tttgacacgc cgcggctcta gtacgcgtca tctttcagct 12480 gtaataacta caaacacatc aaaaaaagtc ggtttaaaac aacttgtgaa acgtcaactt 12540 ggtaaataaa cctattatta aagaatctag atcaatgtcc aaaaagatta tttccattgt 12600 tacgtctaaa tacagattct gcgaaaacaa gccattagta tagtagaaga actaaccatc 12660 gtatgttcgt gaacgtcgag tcaaagtggc tacatgaaca acgattgcca caagcatggt 12720 cagtgtcaga cttctagctt catcgggtcg tctaagagtg gaaagtccat gacgagcaat 12780 tggaatggca atttgaagag gtactcttcc gacagttgga gtggtctccg cctttgccgt 12840 tgtaaccaat ccaataaacc gcatctgtaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn 12900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta gcgcgtccgt caacttcaca 13260 ctggttccgt agtcctaacg ttgtagtctg tgcagatgaa gaacgaggtc aacaacgagg 13320 tcacgtatga gataccgtcg gtagtccatg acacccgaaa ggtcacgtca actaagcaat 13380 tgaagttgca attacctctt cggtactgga aattgggcac ccaaccaagc gaaccgaaac 13440 cacactcgtt acggtggctc gtcgttgacg acctgctggt aaagcagaag gtacgcgaag 13500 ttaacgaacc agaccaagta tcggtcgaat ccgaattgtc ttagttacag tgggacttgc 13560 agtacgacgt atcgttgcca caatgcccgt tattgtcacc acagctaatg gtgaaccaag 13620 aacagccaga ctcatggtca acgtaatcca ctgactgggc cacgatcnnn nnnnnnnnnn 13680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacc accggagggt tgctagaccc 13800 taacgtggga gacgctctat ccacagctac atcattcaga agagaaagaa gagggtcttc 13860 tacaggggac attgcagcta acacgctggt tgttcgttgc gtagacgaca cttgttctgt 13920 cgctaccgag gcaagtaaag tcgccaacgt agtatcgcca atggtaacag tagcaatctg 13980 tcattcacca cgcgaactgg actatgttta aatccctttg ccaatcttcc ctattgtccc 14040 gtcaacttcc ttctgtcttg ctttggtaag tgtcaacgtg gcctgaccga atatcacatt 14100 cagaacagtt gctatgctac ac 14122 <210> 153 <211> 12606 <212> DNA <213> Artificial Sequence <220> <223> SIRV-5 GENE <220> <221> variation <222> 150 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 153 ggaccgaagc atactgcaag acttgtaggc ccatgaatta cccgcttagg caatgggtag 60 acctttcctt gccgcgtgga acaaaccgcc atgagtgttt tacgtttacg gtcggcgacc 120 ggagtcacag cgcgaccaac ggggccaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nnnnnnnnnn nnnnnnnnnn nnnnngagga agccttttag ccatcgctat gccgccgctc 960 aaacgcgccg ggtgcaaaag cccagagcaa aggcccatag gcttttccat cggcacgcga 1020 ccgattccat caagtatagc cacggtcgta acttcgacgg cggcaatagt ttatggcgct 1080 aacacctttc catcgctgag tccaaacgta tgacgtgagg tgttaggtgc aatccccatt 1140 cgggacggac acgcgagtaa aggtctaaca tgtttcaact ttccagttag tcgccgtcct 1200 atattggcaa gaccggatgg ccgtcaacca gttccattct gactatttgc cacattgatt 1260 aaagaagtag gagcgttagt cggcgcgtgc gcgggactat ttagcgaagg tcgccgtatc 1320 gcgggtggcg gcgtttccgc cataacaaca agcgtttaag gtcagagtcc gcgacgaatg 1380 aaacgccgtg caagcggctt tggtacggcc agtttagcca aagcctgatc cgttcgcctt 1440 gggttatcat gtaccagtca agcggtcatc gcgggcggcg ttatcatgnn nnnnnnnnnn 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnga tcgtttgccc gtaaaactaa ctggcaaaag cctaagtgct 2340 gtttcgggtg caatggtata acttcccaca tttagccgaa tagcgccaac agcgttatta 2400 tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnngaaccaa gcagtgggcg atgtcagcga caattccact 2520 ttggtagagg tcgttctttt gtagaaaaca atctaacata actatgacac tttcggtaaa 2580 ttcaccacct ttaccagggt cgtaagtggt caagccacgc cgaccacttc ataagcattg 2640 gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4380 gaaatcgcat cgacacaata aaagtacacc aaccagcaga aaacaggtac atatacagtt 4440 taacgacctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatctaa gatgactaca 4560 gtagcatagc catagaacat ggcactgaaa tatcgatggg cgtaaaagtg aacaacgcag 4620 acgatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5100 nnnnnnnnnn ngattacgac gccaagccac tataaatgct aattgagtcg aagaaggtac 5160 tcctttttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa cacagatatt 5340 gcagaagcgg gaatgaggtt gatattacat caagtatcaa cagacgacct cagaagggtg 5400 gtcttttaaa atgacaacgt acattaagat gcagacaaac actactatgc tgnnnnnnnn 5460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5640 nnnnnnnnnn nnnnnnnnga tgtgaaaatg tcatatgtgc ggcaaaaata gacgacattt 5700 aagaacctag cattatagga tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5820 nnnnnngagt gttgtattaa ttcactctag agtaatggat cacggtgcca gaaactaagg 5880 tggtgtagga ctagatgcaa gtaacaacgg tcgtactaaa atcagctacg cgatcgccga 5940 cttagaaggc tgagatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6120 nnnnnnnnnn nnnnnnnnnn nnnngagaaa cttgtctact ggcagacctc actatctttt 6180 aggaaggtca cgaagcggct ggagtgatgg ctagtctcag ccactgtcaa cgggaagcgc 6240 gacgtttatg gtcaatataa actagcagtt taatccgtat ggcgatctta gcggatgtta 6300 caggatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6660 nnnnnnnnnn nnnnnnnnnn ngagaagatg acaaagtaga agctagacaa ctatatcaaa 6720 tcgttgtgtg gtcttaaacc gacacagtag cattcatcgt tgnnnnnnnn nnnnnnnnnn 6780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatgtggaag gtcacagcct cgttggagga 6900 gatactacgg tcccgctttt cgtgggtgac aaaggtcgca aaggtttcct tagggaggtc 6960 gtttcccgga tctttatcta catctcctgg gtgaagggga tgatctttct ctacatgtcc 7020 tgggtggagg ggagtttcct tgtatgggtg gaacccaacc cgccggtgag ggtgagttac 7080 ggtcggcggt catatgtggg tatgacgggt atgacgtcat aaatagtcgt tgtcaaaatg 7140 ggaggtggaa gtccttactg tagtcacccg tgaaggtgga gaactggcag gacatctcta 7200 ggtcacctaa acgtgcgaag tgtgcgaaat taccaccgta aattcgtcct actgaaagtg 7260 cttacgcatc ttgagagaga ttgcagagag ccaggtcaaa tacattgaga aatctgtata 7320 tgtactttgg taacgctcta aggtaggtac acgttagtga tataaaaaga aggcgacgac 7380 ggtagcagag gtctagaaac cgcatgaacg accaacttat ttgaagagct agtgcggtgg 7440 aattgggtcg gaagaactta aattgttaga gcagtttatg ggtggtaggt tacatctatt 7500 tacttcaaaa cgcggtgccc tagcccacaa gcattgcttt gtaaaattgt tgaaggtaac 7560 ttgtaaaagc cgaacatgaa cgatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9840 nnnnnnnnnn nnnnnnnnga tagtagaccg ggaagaccaa cgttttttca ctactccgaa 9900 agaacacact gaagggaacg gtgtaggttc ggtgagtccg ggactaggtg tccatgtcgg 9960 agacctcagt ggtgacgtcc tcgtagtata gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtccacg 10140 ctctaagtcg cttgcccaat tgtggctacc gcattgctca gaacttcggg tactggccaa 10200 ccttcaagaa gcctagtctt ggtgcgtcac cccagtcacg tagcataccc gtcatcatct 10260 atggtcgtag gtccaacaat cgtggcgcca ttgggtacca cttcatcaga gctgctcgca 10320 tgaccagcta tatctggaac atcgctggtg ggtcttatgg tcgcttttgt gggaaccatc 10380 atagtcgcaa gtctggcctt cggtatggaa ctcgtcgtcc cgactacaac catagctagt 10440 ctcggtctcg gctttgttgc aatgcccttc gttactactt caacggtcgt ggtgccggtc 10500 aaaccgctcg ctaaagcaag cgaagaaaca gctaaactta gttgtgtcga aacatcctaa 10560 gtggtcgggt tgctcgtgga gcatgcagct agcccagaac ctttagcttg tgtggtcttc 10620 gtctcggtag tcagaaccag gtcggtctga atggttggac tcgccacatc aactaggtca 10680 gctattgaag tgccaactaa aggtcgtatt gaagcaatcg gtcctatggc aagtcaagtg 10740 acagtcgtag gtatggcact tgccgcgcct ccagaactcg gtcaagctac ctcataactc 10800 ggaagtcgac tgctctaaag cgaagttgtc aaacgaacag ctatgccttc gagtaaagca 10860 aggtgcgcaa tagtaacctt ggtaacttcg gaactgcgtc aagccgctaa ctccatcggt 10920 atggaagtcg ctaaagtcgg tcaaacggaa gcggctatcg tcgcaactag tcgaatagtt 10980 gtcgaagagg ccagagcaac catggttgcc attgtcttcg tggtctatgc aagacgtcct 11040 tgcgtcatgg cctaagcatc ttcttcactg gttgctatag cggaagtcgt taccgaactg 11100 gtcgccagtc gaatcgtttg ccccatggta gtcatcacca ccatcgcgga aaccaacggt 11160 ccgaactcac ggtaggtagt ccacgtatgt agcccacctt tcgaaggaat cgtggtcgcc 11220 ggtctcctag aaacggtata tgtcttgcgt cacgcttatg gaccggtcat cctggccagt 11280 agccgacttg tggtcgctat agctttcgct agtccctgcc ttaacgtgca gcatggtatg 11340 gctattgtcg tcccggctac aaccactatc gtggtcttga aactggtcgt cgctacaacc 11400 atcctagcat tggcaccagc ggtcgttgaa gccgaaggta gtctctaact ggttttcgaa 11460 gctagtcaaa cagtagcgcc atatggccta gcctgaacct tagaaaaagg agtcgaaggt 11520 cctaaacccg ctggtcgcga acgaacaaaa acggtcgtta aaggccgtcc ggaagcggaa 11580 ggtcgtcgcc ccaggtgaac aagtcctaaa gaaacaaaaa gtctctgtag tctatttaaa 11640 acccaacgca aacgcgaacg cagaatcgtc tgaacccacg tccatcatta cggattatat 11700 aaggtttctg tttcgttaac taggtagtta ttgatgtcat ccgcactaca agctaacagg 11760 aggtaacaga tagagcatga gtggtagtag ggatgaaaca ggaagtacta attacataca 11820 gggactcctg gttgggtgac cccgggacac gtacgtgtcc taataaacag gaggcgggtg 11880 gtttcctatc tactacggac agggattttc cccgagtagc gaaggatatg aaacttctag 11940 ttatagacat ataagacgta gtccaggaag tcggctgagt ccatgaggtc agttttggag 12000 gagaagtagt tgtcacgatc cttagttaag ataacaaagt ccgagtagtt atcacgactt 12060 gctgactgcg tccactatgt ttccggggtg ggcatgggga cttgcacacg gataactcca 12120 tccggattat taagacgagg tggggtgtgc tgggctccca ggaacttcat cggagtgacg 12180 tagaaccttc agtttgagtc cacgtaggac accagcgcta ttggtgccga cgacgagtac 12240 aaacatccgg aaacttagca tattaggtaa gagtttctgt atgctcggac gtcgtcgtta 12300 gagcagaccc agaaggtgtc catggacaca catcatacaa gacaacttgg tcaactttta 12360 cggggctggg gccaattatc ccgggagtcc tcacgatcag aaaccgactg gtttgtcaca 12420 ctctggttag aggaggggtc accacgatgg gtgagggggt ggtgggtgtt tatacaagag 12480 tctgggactc caaagacgat attacgacga gacgacttat ccacgtactt tgtttcccaa 12540 acccacgcaa ctccaccttt agtccacaca aactagtacg agacacccgg attcaaggtc 12600 gtgacc 12606 <210> 154 <211> 10837 <212> DNA <213> Artificial Sequence <220> <223> SIRV6-GENE <220> <221> variation <222> 187 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 154 ggcaagacga atctgcgtca acgctaaagg ccgtcaaaaa gcaagagggc gtcgcgcctg 60 ggaaaaaggt agtcaagaaa ccatcaagct taaatgtcgg gaaattgggt ttgcgccgcc 120 ttcaggggca gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact 180 cctcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnggtagtt gttgaaaaag taggaatatc tagcctagat gtcgtttcag gcgaaggact 360 ctaaggtctt aaaatcgttg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt caaccgctct 480 aaagcgaagt tgttggacaa acagttatgc gtaactgtaa agcaaggtgc ccaagtagac 540 tcttactaat ttgggaacga cggaaagtcg ctaccttcat cggtatggct ttcgctaaag 600 tcggtcaaat ggaagaaggt agtcgaagta ctgttgaaag agtagactga gcgacagtcg 660 aaaccagccc caatgaacaa gacgccattg caaaacgtct atacgctacg gtcaaagacg 720 cttccacacc acatggtata gtggacgccg ttagcctcat gtgtctcact caaagacctt 780 gcgtcacggt catcctcttt gtcagcaacg tagaaatctc cggtctgtcg ataccttgcg 840 taaagctctc atgtagccta ccttacgaag aaatcgcaca agtcggtcgc ccagcggtgg 900 catatgtctt gcctaacgtt tctagaccga tcagcctcac gagtagcctg cttgtggtgt 960 ttatagttta cgctagtcca atctgtatcg tgccgcttgg tatggctatt gtcggcctgg 1020 atgcaagcac taacgtggtc atcagactgg tctgcgctat aatctacgca atagaggcac 1080 cagcggtcgt tgaagccgaa gacgctattt cggtggcttg nnnnnnnnnn nnnnnnnnnn 1140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnnnnn nnnnngaata ttgtttcaat ggtcgtggcg ctggtcagac 1320 tgctcgctaa agtcgtcgaa gaaacagcta gaccatctat tctctgaact tcctaagtgg 1380 tcgggttggt cgtggcgctc atagttagcc cagaacgtcc agtttatgtg gtggtgatct 1440 tggttgccag actcagtatg gtcaaaatag ttgtgctcgc catttcaact agtgcagttg 1500 ctaaagtgcc aactaaggtt cgtcttgaag caatcggtag tatctgcagt caagtcgtag 1560 tcgtaggtat ggcacttgtc gtgcgtctag gacgcgttga agctatctta ttgcccgaag 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 gagccgaaag tctgggacac gtcgtgggac gtatcgttag gactatggga ctagtcgtct 1800 tcggtcttac aaatgttgca atgccctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcctatg cctcgtatca 2040 tctatggtcg taggtaaagc aatagcggaa acattggatg tgacttcatc aggactgctc 2100 gtatagtgag ctacttatga aacatgaatg gtcggtcttg tggtcgcttt tgtcgaaatc 2160 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaaaca ttaaatcaaa 6120 gaagtttatc aaccaattag tacagtggca tgttaagtaa aacagaaaga acgccaagtg 6180 gcaacaaatg acgtaattat ttaagcaaaa agcgtaaacg cttacaaagg ctatttaaat 6240 atcagtaaat cgttggaaat tgggtatgtg ctgccttcat acgcaaaaat ggatgcgaca 6300 gaaacgagtt gtatggcctc cactacgctc gcctgccgcg gaccctaaac gacagtcgtt 6360 tgcccaacta cggctacggc atcgctcaga acttcgggtc ttggcccacc tttctgacgc 6420 ctagtcaacg tgcgtcgcct cagtcttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6780 nnnnnnnnnn nnnnnnnnnn nnnnngacaa aaagaagaaa tatcaattgc aaaaataact 6840 ataggtactt ctttgaagaa accaaacgtg agtttctaac acgactaaat ccatttgatc 6900 tttgagaaag tcgaagacga atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7980 nnnnnnnnnn nnnnnnnnng tgggtttata tcgcgtatcc atccaactta actgaaacgc 8040 gaagcagtag acagtgttga tcgagatttg cgaagggaaa cagtaaaagt ccagggtgtc 8100 gttaattacg acaaaactaa agactgtata gttagaagga tcaattgggc tgatttagaa 8160 atggtataag ttaaagaaat ggaaacttat tcgacaaaaa gaaaaactca tgatgtatag 8220 atggaaactg ccataatgtc gattcgagtt atatttttag attttggtaa aatatccaac 8280 atgtattaac gaaattttga agtatagatc aaactattct tcaggtacag catcaagcat 8340 tcggtagcat aagccaaaca agtctttgaa gggttggaca tagcgagttg tgtagaaagt 8400 tacgaagact tcaacagaaa aagagtttaa aaggaccacg tatgccaaat cgacaacaat 8460 atagtcgttc tattctgcga tatgtaagat agagacacat cggcaggtag catagaactt 8520 gggtcaatta ttatttaagt gcggtaacaa acaaacttag ggaatattat tgtgctacag 8580 caaatgcctt gaaaagtcga gaaagactac tatactcgtc aagttcgtaa agacttaaca 8640 gtaattgtaa tgcaaatact tagcgagatt gattccttcc cagtagtcat ctttccttta 8700 gatattagaa cagcaatttt agattttgta catagtggag aaaaggacgt cattttaatg 8760 gatgaaccag acgttaacgt catttacttg gtcgttgatt tcgaagactc agtcatcaaa 8820 attgtagctt cgctagctaa tgtgcttaaa atcatcatag tcaaagtgta cgtggtcgtg 8880 gtaataaaca accttcatta ctgtacaaaa gtgccagttt gttacattat cgaacgctgt 8940 atagaggtat catttcacct atttgacaac taggtgataa accttcggtc gctagacgtt 9000 gttgttattg aaaaaatgga agataatcta tacttaatcc aaaacaaccc agaccaagct 9060 cgtatataaa tggaagtcgt caaaattggt ttcgttcaaa taagttggga ctgatgaatc 9120 agttttctga acttcaccgc tattagcgtc ctagttgtct acataattag tatcattatt 9180 gtagtagttt aaatcgctaa caaccaaaaa atccttgaca tgggtaacga cgttattggg 9240 gaaccaatgc tcagtgttgg tgaaagaatc gttaggtcga actattggta cgttctaata 9300 aaagacgaca tagaaattcg ttacataatc aacgtcacta ctaagggcgt attgcaaatt 9360 agttcataaa ttattaacag tttgcaccta atagttctag tccttaaaaa ggtagaagac 9420 gtaggtaatg tcagttgcct caatgtagtc aggtaaaaag tatatgtgct gatggcaaac 9480 ttcatctttg tgagagttga gcatgtataa caacatcact cagtataacc gcttggttac 9540 acaattagta tcatgcctat agaacgcctt atcattcagg tcgtcgtgat agtgaagggg 9600 atcaaagtca aaatctactt tgtcataact ctttacggta tcccaatgct tggtataagt 9660 gcttaagaaa ttgtttaaac agaaactcaa gtataagcga aaacaagtta cctggaaaaa 9720 gaagaagtgc gtataattga tgaacgagtc gaagtcgaag ttgttcaaca agagagttag 9780 gtagtgccgc ttgaaaaagt aatagtgcaa ataagaaagt aagactgtat cggagacgac 9840 tttttaatag tagtaattct cggtacaact aaaccagtaa tttcagacac tgaaatagag 9900 gacttcgtta ggtacgaagc aagttaacta aatgacaatt aaattgaggg taaatatcac 9960 aaagaaatca tggaagattt cgaccgaata caggaaaatt tagattaaag gtttgtcgtc 10020 gttgaaataa gaagaagcga aacggcattc gtttaacaaa acgtcacata tttaggtata 10080 gtagattgcg ttcacaaaga aatttaaagt cttgtaatta tggtggtcga tttattggta 10140 gtatatttgg aaatagcatg caattttgga atggacgaag aacctgacat ctgagctatt 10200 gtaagtttaa tttaccattt cgtcattgta tatgcggtaa acgtaaattt acacatatga 10260 cacgaacttt gagttttatt agcctgccta attgttggta acaatgtaat ggaaattggc 10320 gatgactatg gggaaagagc aatcaactca ccataaattt gtgaacgcag ttaagtaatt 10380 ggcatcgcgc gcctaatcgt ttacattcgc atggaacaag aagaaagaga cctattcgtg 10440 ggagtcaatg gctatgtgga aaaacatgaa ctgccaaatt cggctaacca accagaaatg 10500 gtcatggaaa attcttgcgt caaagttcgc ggtgacgttt acgaagaagc taaacttgtg 10560 cttgtttaga aagttaggtg cgtcctggtt ttagtaaatt tagaaaacca ttttgttgtt 10620 tgcgacagtt aaataaaact taacgacaaa gaagtgactt gcgtcgtaga ttaactggaa 10680 attaaagtgg tatctaaaat ggggtttgcc gatgcggtct tcaaagtcta aaattcttga 10740 agtttgcgtt tggtcgcaaa aattatggat ttagtcgatt attgcctact aagcagcagt 10800 ttagaagttt ttaagatagt tacgctagaa gaccggg 10837 <210> 155 <211> 147317 <212> DNA <213> Artificial Sequence <220> <223> SIRV7-GENE <220> <221> variation <222> 350 <223> for this and all following instances of 'n': n = any of a,c,g,t <400> 155 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtgn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 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nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 26820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 26880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 26940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aatcgaacaa catatctatt taattagttt 33360 aataacaaat taagtgactt atcgcggaag agagaacaat agattggatt aaaaagttgt 33420 agtaaaccac aacgaagtta ggtagctggt aaggttatag atgcgttctg tcgttgtagt 33480 cccaatgaac atagtatggt ttcgcatagt aactgaccta aaagaccgcc acatagtgaa 33540 aatacttaaa acgcaagttt cataggatta agtggtagag gctatagaaa atgatatcat 33600 aactataacg aatataacag caaattaaaa tgcactgnnn nnnnnnnnnn nnnnnnnnnn 33660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 35040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 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nnnnnnnnnn 69120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngataat 69360 aaaaattaaa ctaatatttg tagttagtgg tgcagatttg aacgaacagt caggcgaaat 69420 ggaagtaata gatgattcat ggacgataag tcattaagac atcgaagact gttttgnnnn 69480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 69960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 70980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 71940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 72960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 73980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 74940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gatgtagaca 77340 tatttgtaga acattaaaga aagatatgag ttctttattt caaaaacata gatggttggc 77400 tggttagcaa cgtcaaagcc cacagtatct aatgnnnnnn nnnnnnnnnn nnnnnnnnnn 77460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 77940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 78000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 78060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 78120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 78180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 78240 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nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 91980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92220 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 92280 aaaaatccta agagatcgac gaagttatcg actaaattgt tgtaatccaa gaggctgatt 92340 gacgtttaga agtaagtggc aatttggttt aaaacaacga taataaaggt tgcgaagaaa 92400 tataaaaagt agtcacataa cactgacatt attaaattta gatagacttt accgctaaaa 92460 ctgtagttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 92940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 93000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 93060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 93120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 93180 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nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 133980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatttcgaa gaacgcgaag aactaaacga 135780 acaagttatg aacgaaatta gagtcctaat aacctgttta gtgaattgac cagtagtcat 135840 ataacaaaaa caagaaggtt aggcaaagct ataagcaaat agcattgaaa tagaaatgnn 135900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 136980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 137940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 138960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 139980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140280 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 140940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142740 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142800 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142920 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142980 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacgagaaga aggctttaaa 143220 atggtagtcg tatgcgaacg ctaaggaaga gaaattctag tagaactcgt agttacatac 143280 ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143340 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143460 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac gaaagaaaag 143520 tcgcaaagat cggaaatcaa gacgcaaaac aaaacggaga agttcaaatc gaactcgtag 143580 aacatatcgc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143640 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144120 nnnnnnnnnn nnnnnnnnga acatattaaa ctcttggtat cgctcagtta caaaaggaga 144180 cacgtagaca cgaaagaaac tggaacaaca gacatatttg tgnnnnnnnn nnnnnnnnnn 144240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144420 ngatgttaga gcaaattata taaactttca catgctaacc aacgtaattt gagaagaaat 144480 catcaagaat taaactaaag gaatggtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144540 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144600 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145200 nnnnnngata atggcaatta tagttaaaat agtcgtaaat tagattatgg tgcaccgggg 145260 tgtaagtttc gaactcggtg aggcaatagt agtatagact aacaacaagg tagacattgc 145320 aatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145560 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145620 nnnnnnnnnn nnnnnnnnnn nntattgcga gaagaacttc acgaagatat agatacagta 145680 cagaatttct aggattaagt acagcagacg tatttacacc tgttatagga tacttgttca 145740 cttaatccct gttatgacaa agtagtctca tagaattttt agaacgagaa gatttgaatg 145800 gacgtaaagg aagaaggtca aaatggagaa cttaaaaggg gaaatctaga acattgaacc 145860 caacattata caatgatata agaacgaaaa gacgaagaca ataaggtcca tgggatttac 145920 ccaaatgagg acctaaatca ccgggaagaa gtaagtaaac ataacgatat agttttagat 145980 tatcgagaag tcgaagaaga ccgggatagg taacagataa tcgacgtttg ggaagacggt 146040 aacgtaagaa actgaggagg ttaaagaact aagaatcaac aacaagaaac tattgtccct 146100 attctactta ggtatctgct agtatgcgat atggaagtta aagaggaaac agtagtatgc 146160 actgtttgag aaaattagga tttaagaggt ggagaagtcg ttagtttgga agcatagata 146220 gttcaaatat ccacagaagg ctattaaaag atagaagaga gtcgagagat cgattttaaa 146280 gtctgagtcg aaatagtttg gtgcaagatt atccattcaa ttcttatcga aaacttacaa 146340 ggtcaacaag tagagatagc aatataacta aaagctatct attgaacata aatgcgcgac 146400 tacagcttgc gggttaacac ggtcgtcttc attatacgtt taatcgacgc cggcttattc 146460 atgcagatat ttaactcaat cttcgatagg atagaggacg tagtagactt ggatgtctgt 146520 catccagaca tgagacatat acttatatgg ataatggtag tgcagcaaag tcgtataatt 146580 taaacggtat gggacgaagc taacaacata gaggtggaca acgtatagca ggtaattgtt 146640 aaacttgttc tggaaataaa gaaagaaatc ttttggacta tgggcgtggc attagtatca 146700 agagtagtct tggtttgtat tatattggca gtgagcttat tcgtaataga cgtaatctgt 146760 cgcacttacg tttagccaaa ttaggtccgt gaaacagaca caaacctata tttatgtggc 146820 cacggacaaa cgaaaactat aagctacttc gcattctgag gtcttgttgg gctaataata 146880 gactgcaatc acgttgctgg ggtggtatcc ttaactaaca atatggtaag tacggtgata 146940 gtgacattca aaatagggtg gtaacttagc atggtctact tggtggcaga agtggaagtg 147000 gtagttatat tcagctttat ggttttacac tgtatatttc acttggcggt cgccacaata 147060 aagatttaga atccactggc agaaagtcag cgcattttta tagcagacaa cttgcaaagg 147120 gagaggttca tgcaacgtgt tatggtggat caagttatct tagtaagaca taaacttgta 147180 tagacaataa cagtggcgga aattaaggtt aagataattg taactatagt tccgggagag 147240 aactatttaa ttccgacaac tgttatagcc aaaatagatg aaatagagcg tataaaacct 147300 attcttccaa gcgtaat 147317 <210> 156 <211> 248 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 156 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 60 aaaaatccta agagatcgac gaagttatcg actaaattgt tgtaatccaa gaggctgatt 120 gacgtttaga agtaagtggc aatttggttt aaaacaacga taataaaggt tgcgaagaaa 180 tataaaaagt agtcacataa cactgacatt attaaattta gatagacttt accgctaaaa 240 ctgtagtt 248 <210> 157 <211> 66 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 157 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 60 aaaaat 66 <210> 158 <211> 65 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 158 ttgttatggc agaaacagac aaaatatcag gctacaaaaa tgtatcagag cattaacatg 60 aaaaa 65 <210> 159 <211> 104 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 159 gatgtagaca tatttgtaga acattaaaga aagatatgag ttctttattt caaaaacata 60 gatggttggc tggttagcaa cgtcaaagcc cacagtatct aatg 104 <210> 160 <211> 68 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 160 gtcaggcgaa atggaagtaa tagatgattc atggacgata agtcattaag acatcgaaga 60 ctgttttg 68 <210> 161 <211> 122 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 161 gataataaaa attaaactaa tatttgtagt tagtggtgca gatttgaacg aacagtcagg 60 cgaaatggaa gtaatagatg attcatggac gataagtcat taagacatcg aagactgttt 120 tg 122 <210> 162 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 162 ggctatagaa aatgatatca taactataac gaatataaca gcaaattaaa atgcactg 58 <210> 163 <211> 236 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 163 gattggatta aaaagttgta gtaaaccaca acgaagttag gtagctggta aggttataga 60 tgcgttctgt cgttgtagtc ccaatgaaca tagtatggtt tcgcatagta actgacctaa 120 aagaccgcca catagtgaaa atacttaaaa cgcaagtttc ataggattaa gtggtagagg 180 ctatagaaaa tgatatcata actataacga atataacagc aaattaaaat gcactg 236 <210> 164 <211> 276 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 164 gtttaataac aaattaagtg acttatcgcg gaagagagaa caatagattg gattaaaaag 60 ttgtagtaaa ccacaacgaa gttaggtagc tggtaaggtt atagatgcgt tctgtcgttg 120 tagtcccaat gaacatagta tggtttcgca tagtaactga cctaaaagac cgccacatag 180 tgaaaatact taaaacgcaa gtttcatagg attaagtggt agaggctata gaaaatgata 240 tcataactat aacgaatata acagcaaatt aaaatg 276 <210> 165 <211> 308 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 165 gaatcgaaca acatatctat ttaattagtt taataacaaa ttaagtgact tatcgcggaa 60 gagagaacaa tagattggat taaaaagttg tagtaaacca caacgaagtt aggtagctgg 120 taaggttata gatgcgttct gtcgttgtag tcccaatgaa catagtatgg tttcgcatag 180 taactgacct aaaagaccgc cacatagtga aaatacttaa aacgcaagtt tcataggatt 240 aagtggtaga ggctatagaa aatgatatca taactataac gaatataaca gcaaattaaa 300 atgcactg 308 <210> 166 <211> 310 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 166 gatgaaactt gagaaatcgc actggcttac atatacacga taaaagactt taatacaata 60 taagaagtcg ttattgaaga cggagatgta tgttaccgcc caattttaga gatacaaaat 120 gagactgaca tagcggttaa ctatattaga gcacgcctaa gcattgaagg tttagtcatc 180 ttcattagag tatactgtat tgacggctga atgcaaataa attgagacaa aaccgatttc 240 ttgaattagc acgaagtaag tataaaacta gtagagtgac accaagtata tagggggttt 300 atatcgcgtg 310 <210> 167 <211> 349 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 167 gtgccaaatt agcgaacaat ttgagaagag cagtgagacg atgaaacttg agaaatcgca 60 ctggcttaca tatacacgat aaaagacttt aatacaatat aagaagtcgt tattgaagac 120 ggagatgtat gttaccgccc aattttagag atacaaaatg agactgacat agcggttaac 180 tatattagag cacgcctaag cattgaaggt ttagtcatct tcattagagt atactgtatt 240 gacggctgaa tgcaaataaa ttgagacaaa accgatttct tgaattagca cgaagtaagt 300 ataaaactag tagagtgaca ccaagtatat agggggttta tatcgcgtg 349 <210> 168 <211> 1675 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 168 tattgcgaga agaacttcac gaagatatag atacagtaca gaatttctag gattaagtac 60 agcagacgta tttacacctg ttataggata cttgttcact taatccctgt tatgacaaag 120 tagtctcata gaatttttag aacgagaaga tttgaatgga cgtaaaggaa gaaggtcaaa 180 atggagaact taaaagggga aatctagaac attgaaccca acattataca atgatataag 240 aacgaaaaga cgaagacaat aaggtccatg ggatttaccc aaatgaggac ctaaatcacc 300 gggaagaagt aagtaaacat aacgatatag ttttagatta tcgagaagtc gaagaagacc 360 gggataggta acagataatc gacgtttggg aagacggtaa cgtaagaaac tgaggaggtt 420 aaagaactaa gaatcaacaa caagaaacta ttgtccctat tctacttagg tatctgctag 480 tatgcgatat ggaagttaaa gaggaaacag tagtatgcac tgtttgagaa aattaggatt 540 taagaggtgg agaagtcgtt agtttggaag catagatagt tcaaatatcc acagaaggct 600 attaaaagat agaagagagt cgagagatcg attttaaagt ctgagtcgaa atagtttggt 660 gcaagattat ccattcaatt cttatcgaaa acttacaagg tcaacaagta gagatagcaa 720 tataactaaa agctatctat tgaacataaa tgcgcgacta cagcttgcgg gttaacacgg 780 tcgtcttcat tatacgttta atcgacgccg gcttattcat gcagatattt aactcaatct 840 tcgataggat agaggacgta gtagacttgg atgtctgtca tccagacatg agacatatac 900 ttatatggat aatggtagtg cagcaaagtc gtataattta aacggtatgg gacgaagcta 960 acaacataga ggtggacaac gtatagcagg taattgttaa acttgttctg gaaataaaga 1020 aagaaatctt ttggactatg ggcgtggcat tagtatcaag agtagtcttg gtttgtatta 1080 tattggcagt gagcttattc gtaatagacg taatctgtcg cacttacgtt tagccaaatt 1140 aggtccgtga aacagacaca aacctatatt tatgtggcca cggacaaacg aaaactataa 1200 gctacttcgc attctgaggt cttgttgggc taataataga ctgcaatcac gttgctgggg 1260 tggtatcctt aactaacaat atggtaagta cggtgatagt gacattcaaa atagggtggt 1320 aacttagcat ggtctacttg gtggcagaag tggaagtggt agttatattc agctttatgg 1380 ttttacactg tatatttcac ttggcggtcg ccacaataaa gatttagaat ccactggcag 1440 aaagtcagcg catttttata gcagacaact tgcaaaggga gaggttcatg caacgtgtta 1500 tggtggatca agttatctta gtaagacata aacttgtata gacaataaca gtggcggaaa 1560 ttaaggttaa gataattgta actatagttc cgggagagaa ctatttaatt ccgacaactg 1620 ttatagccaa aatagatgaa atagagcgta taaaacctat tcttccaagc gtaat 1675 <210> 169 <211> 118 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 169 gataatggca attatagtta aaatagtcgt aaattagatt atggtgcacc ggggtgtaag 60 tttcgaactc ggtgaggcaa tagtagtata gactaacaac aaggtagaca ttgcaatg 118 <210> 170 <211> 87 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 170 gatgttagag caaattatat aaactttcac atgctaacca acgtaatttg agaagaaatc 60 atcaagaatt aaactaaagg aatggtg 87 <210> 171 <211> 84 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 171 gaacatatta aactcttggt atcgctcagt tacaaaagga gacacgtaga cacgaaagaa 60 actggaacaa cagacatatt tgtg 84 <210> 172 <211> 85 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 172 gacgaaagaa aagtcgcaaa gatcggaaat caagacgcaa aacaaaacgg agaagttcaa 60 atcgaactcg tagaacatat cgctg 85 <210> 173 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 173 gaagtggaaa atttcagcaa gagagtcgaa gtcgtcatag attagcatg 49 <210> 174 <211> 95 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 174 gaagtgcgta taattgatga acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta 60 gtgccgcttg aaaaagtaat agtgcaaata agaaa 95 <210> 175 <211> 65 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 175 gaagtgcgta taattgatga acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta 60 gtgcc 65 <210> 176 <211> 385 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 176 gaagtgcgta taattgatga acgagtcgaa gtcgaagttg ttcaacaaga gagttaggta 60 gtgccgcttg aaaaagtaat agtgcaaata agaaagtaag actgtatcgg agacgacttt 120 ttaatagtag taattctcgg tacaactaaa ccagtaattt cagacactga aatagaggac 180 ttcgttaggt acgaagcaag ttaactaaat gacaattaaa ttgagggtaa atatcacaaa 240 gaaatcatgg aagatttcga ccgaatacag gaaaatttag attaaaggtt tgtcgtcgtt 300 gaaataagaa gaagcgaaac ggcattcgtt taacaaaacg tcacatattt aggtatagta 360 gattgcgttc acaaagaaat ttaaa 385 <210> 177 <211> 98 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 177 gaagaagtgc gtataattga tgaacgagtc gaagtcgaag ttgttcaaca agagagttag 60 gtagtgccgc ttgaaaaagt aatagtgcaa ataagaaa 98 <210> 178 <211> 581 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 178 ggggaaccaa tgctcagtgt tggtgaaaga atcgttaggt cgaactattg gtacgttcta 60 ataaaagacg acatagaaat tcgttacata atcaacgtca ctactaaggg cgtattgcaa 120 attagttcat aaattattaa cagtttgcac ctaatagttc tagtccttaa aaaggtagaa 180 gacgtaggta atgtcagttg cctcaatgta gtcaggtaaa aagtatatgt gctgatggca 240 aacttcatct ttgtgagagt tgagcatgta taacaacatc actcagtata accgcttggt 300 tacacaatta gtatcatgcc tatagaacgc cttatcattc aggtcgtcgt gatagtgaag 360 gggatcaaag tcaaaatcta ctttgtcata actctttacg gtatcccaat gcttggtata 420 agtgcttaag aaattgttta aacagaaact caagtataag cgaaaacaag ttacctggaa 480 aaagaagaag tgcgtataat tgatgaacga gtcgaagtcg aagttgttca acaagagagt 540 taggtagtgc cgcttgaaaa agtaatagtg caaataagaa a 581 <210> 179 <211> 96 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 179 agtagactga gcgacagtcg aaaccagccc caatgaacaa gacgccattg caaaacgtct 60 atacgctacg gtcaaagacg cttccacacc acatgg 96 <210> 180 <211> 95 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 180 agtagactga gcgacagtcg aaaccagccc caatgaacaa gacgccattg caaaacgtct 60 atacgctacg gtcaaagacg cttccacacc acatg 95 <210> 181 <211> 310 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 181 agtagactga gcgacagtcg aaaccagccc caatgaacaa gacgccattg caaaacgtct 60 atacgctacg gtcaaagacg cttccacacc acatggtata gtggacgccg ttagcctcat 120 gtgtctcact caaagacctt gcgtcacggt catcctcttt gtcagcaacg tagaaatctc 180 cggtctgtcg ataccttgcg taaagctctc atgtagccta ccttacgaag aaatcgcaca 240 agtcggtcgc ccagcggtgg catatgtctt gcctaacgtt tctagaccga tcagcctcac 300 gagtagcctg 310 <210> 182 <211> 1969 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 182 gtgggtttat atcgcgtatc catccaactt aactgaaacg cgaagcagta gacagtgttg 60 atcgagattt gcgaagggaa acagtaaaag tccagggtgt cgttaattac gacaaaacta 120 aagactgtat agttagaagg atcaattggg ctgatttaga aatggtataa gttaaagaaa 180 tggaaactta ttcgacaaaa agaaaaactc atgatgtata gatggaaact gccataatgt 240 cgattcgagt tatattttta gattttggta aaatatccaa catgtattaa cgaaattttg 300 aagtatagat caaactattc ttcaggtaca gcatcaagca ttcggtagca taagccaaac 360 aagtctttga agggttggac atagcgagtt gtgtagaaag ttacgaagac ttcaacagaa 420 aaagagttta aaaggaccac gtatgccaaa tcgacaacaa tatagtcgtt ctattctgcg 480 atatgtaaga tagagacaca tcggcaggta gcatagaact tgggtcaatt attatttaag 540 tgcggtaaca aacaaactta gggaatatta ttgtgctaca gcaaatgcct tgaaaagtcg 600 agaaagacta ctatactcgt caagttcgta aagacttaac agtaattgta atgcaaatac 660 ttagcgagat tgattccttc ccagtagtca tctttccttt agatattaga acagcaattt 720 tagattttgt acatagtgga gaaaaggacg tcattttaat ggatgaacca gacgttaacg 780 tcatttactt ggtcgttgat ttcgaagact cagtcatcaa aattgtagct tcgctagcta 840 atgtgcttaa aatcatcata gtcaaagtgt acgtggtcgt ggtaataaac aaccttcatt 900 actgtacaaa agtgccagtt tgttacatta tcgaacgctg tatagaggta tcatttcacc 960 tatttgacaa ctaggtgata aaccttcggt cgctagacgt tgttgttatt gaaaaaatgg 1020 aagataatct atacttaatc caaaacaacc cagaccaagc tcgtatataa atggaagtcg 1080 tcaaaattgg tttcgttcaa ataagttggg actgatgaat cagttttctg aacttcaccg 1140 ctattagcgt cctagttgtc tacataatta gtatcattat tgtagtagtt taaatcgcta 1200 acaaccaaaa aatccttgac atgggtaacg acgttattgg ggaaccaatg ctcagtgttg 1260 gtgaaagaat cgttaggtcg aactattggt acgttctaat aaaagacgac atagaaattc 1320 gttacataat caacgtcact actaagggcg tattgcaaat tagttcataa attattaaca 1380 gtttgcacct aatagttcta gtccttaaaa aggtagaaga cgtaggtaat gtcagttgcc 1440 tcaatgtagt caggtaaaaa gtatatgtgc tgatggcaaa cttcatcttt gtgagagttg 1500 agcatgtata acaacatcac tcagtataac cgcttggtta cacaattagt atcatgccta 1560 tagaacgcct tatcattcag gtcgtcgtga tagtgaaggg gatcaaagtc aaaatctact 1620 ttgtcataac tctttacggt atcccaatgc ttggtataag tgcttaagaa attgtttaaa 1680 cagaaactca agtataagcg aaaacaagtt acctggaaaa agaagaagtg cgtataattg 1740 atgaacgagt cgaagtcgaa gttgttcaac aagagagtta ggtagtgccg cttgaaaaag 1800 taatagtgca aataagaaag taagactgta tcggagacga ctttttaata gtagtaattc 1860 tcggtacaac taaaccagta atttcagaca ctgaaataga ggacttcgtt aggtacgaag 1920 caagttaact aaatgacaat taaattgagg gtaaatatca caaagaaat 1969 <210> 183 <211> 278 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 183 ttactaattt gggaacgacg gaaagtcgct accttcatcg gtatggcttt cgctaaagtc 60 ggtcaaatgg aagaaggtag tcgaagtact gttgaaagag tagactgagc gacagtcgaa 120 accagcccca atgaacaaga cgccattgca aaacgtctat acgctacggt caaagacgct 180 tccacaccac atggtatagt ggacgccgtt agcctcatgt gtctcactca aagaccttgc 240 gtcacggtca tcctctttgt cagcaacgta gaaatctc 278 <210> 184 <211> 118 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 184 gacaaaaaga agaaatatca attgcaaaaa taactatagg tacttctttg aagaaaccaa 60 acgtgagttt ctaacacgac taaatccatt tgatctttga gaaagtcgaa gacgaatg 118 <210> 185 <211> 267 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 185 gtcaaccgct ctaaagcgaa gttgttggac aaacagttat gcgtaactgt aaagcaaggt 60 gcccaagtag actcttacta atttgggaac gacggaaagt cgctaccttc atcggtatgg 120 ctttcgctaa agtcggtcaa atggaagaag gtagtcgaag tactgttgaa agagtagact 180 gagcgacagt cgaaaccagc cccaatgaac aagacgccat tgcaaaacgt ctatacgcta 240 cggtcaaaga cgcttccaca ccacatg 267 <210> 186 <211> 73 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 186 gtcaaccgct ctaaagcgaa gttgttggac aaacagttat gcgtaactgt aaagcaaggt 60 gcccaagtag act 73 <210> 187 <211> 78 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 187 ggtagttgtt gaaaaagtag gaatatctag cctagatgtc gtttcaggcg aaggactcta 60 aggtcttaaa atcgttgg 78 <210> 188 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 188 gggcgacaat tgtggaccgt atggactcca ttatgctggg actcctcgg 49 <210> 189 <211> 56 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 189 gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgg 56 <210> 190 <211> 62 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 190 ggggcagcga tgggggcgac aattgtggac cgtatggact ccattatgct gggactcctc 60 gg 62 <210> 191 <211> 99 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 191 gcttaaatgt cgggaaattg ggtttgcgcc gccttcaggg gcagcgatgg gggcgacaat 60 tgtggaccgt atggactcca ttatgctggg actcctcgg 99 <210> 192 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 192 tgagctactt atgaaacatg aatggtcggt cttgtggtcg cttttgtcga aatcaccg 58 <210> 193 <211> 59 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 193 gtgagctact tatgaaacat gaatggtcgg tcttgtggtc gcttttgtcg aaatcaccg 59 <210> 194 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 194 ggactatggg actagtcgtc ttcggtctta caaatgttgc aatgccctg 49 <210> 195 <211> 88 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 195 gagccgaaag tctgggacac gtcgtgggac gtatcgttag gactatggga ctagtcgtct 60 tcggtcttac aaatgttgca atgccctg 88 <210> 196 <211> 74 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 196 gagccgaaag tctgggacac gtcgtgggac gtatcgttag gactatggga ctagtcgtct 60 tcggtcttac aaat 74 <210> 197 <211> 186 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 197 ggcaagacga atctgcgtca acgctaaagg ccgtcaaaaa gcaagagggc gtcgcgcctg 60 ggaaaaaggt agtcaagaaa ccatcaagct taaatgtcgg gaaattgggt ttgcgccgcc 120 ttcaggggca gcgatggggg cgacaattgt ggaccgtatg gactccatta tgctgggact 180 cctcgg 186 <210> 198 <211> 104 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 198 ggttcgtctt gaagcaatcg gtagtatctg cagtcaagtc gtagtcgtag gtatggcact 60 tgtcgtgcgt ctaggacgcg ttgaagctat cttattgccc gaag 104 <210> 199 <211> 148 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 199 gtgctcgcca tttcaactag tgcagttgct aaagtgccaa ctaaggttcg tcttgaagca 60 atcggtagta tctgcagtca agtcgtagtc gtaggtatgg cacttgtcgt gcgtctagga 120 cgcgttgaag ctatcttatt gcccgaag 148 <210> 200 <211> 150 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 200 ttgtgctcgc catttcaact agtgcagttg ctaaagtgcc aactaaggtt cgtcttgaag 60 caatcggtag tatctgcagt caagtcgtag tcgtaggtat ggcacttgtc gtgcgtctag 120 gacgcgttga agctatctta ttgcccgaag 150 <210> 201 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 201 ttgtgctcgc catttcaact agtgcagttg ctaaagtgcc aactaaggtt cgtcttgaag 60 caatcggtag 70 <210> 202 <211> 151 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 202 gttgtgctcg ccatttcaac tagtgcagtt gctaaagtgc caactaaggt tcgtcttgaa 60 gcaatcggta gtatctgcag tcaagtcgta gtcgtaggta tggcacttgt cgtgcgtcta 120 ggacgcgttg aagctatctt attgcccgaa g 151 <210> 203 <211> 190 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 203 tattctctga acttcctaag tggtcgggtt ggtcgtggcg ctcatagtta gcccagaacg 60 tccagtttat gtggtggtga tcttggttgc cagactcagt atggtcaaaa tagttgtgct 120 cgccatttca actagtgcag ttgctaaagt gccaactaag gttcgtcttg aagcaatcgg 180 tagtatctgc 190 <210> 204 <211> 335 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 204 gaatattgtt tcaatggtcg tggcgctggt cagactgctc gctaaagtcg tcgaagaaac 60 agctagacca tctattctct gaacttccta agtggtcggg ttggtcgtgg cgctcatagt 120 tagcccagaa cgtccagttt atgtggtggt gatcttggtt gccagactca gtatggtcaa 180 aatagttgtg ctcgccattt caactagtgc agttgctaaa gtgccaacta aggttcgtct 240 tgaagcaatc ggtagtatct gcagtcaagt cgtagtcgta ggtatggcac ttgtcgtgcg 300 tctaggacgc gttgaagcta tcttattgcc cgaag 335 <210> 205 <211> 640 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 205 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 120 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 180 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 240 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 300 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 360 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 420 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 480 gaaattaaag tggtatctaa aatggggttt gccgatgcgg tcttcaaagt ctaaaattct 540 tgaagtttgc gtttggtcgc aaaaattatg gatttagtcg attattgcct actaagcagc 600 agtttagaag tttttaagat agttacgcta gaagaccggg 640 <210> 206 <211> 630 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 206 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 120 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 180 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 240 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 300 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 360 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 420 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 480 gaaattaaag tggtatctaa aatggggttt gccgatgcgg tcttcaaagt ctaaaattct 540 tgaagtttgc gtttggtcgc aaaaattatg gatttagtcg attattgcct actaagcagc 600 agtttagaag tttttaagat agttacgcta 630 <210> 207 <211> 628 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 207 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 120 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 180 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 240 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 300 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 360 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 420 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 480 gaaattaaag tggtatctaa aatggggttt gccgatgcgg tcttcaaagt ctaaaattct 540 tgaagtttgc gtttggtcgc aaaaattatg gatttagtcg attattgcct actaagcagc 600 agtttagaag tttttaagat agttacgc 628 <210> 208 <211> 493 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 208 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 120 ggcgatgact atggggaaag agcaatcaac tcaccataaa tttgtgaacg cagttaagta 180 attggcatcg cgcgcctaat cgtttacatt cgcatggaac aagaagaaag agacctattc 240 gtgggagtca atggctatgt ggaaaaacat gaactgccaa attcggctaa ccaaccagaa 300 atggtcatgg aaaattcttg cgtcaaagtt cgcggtgacg tttacgaaga agctaaactt 360 gtgcttgttt agaaagttag gtgcgtcctg gttttagtaa atttagaaaa ccattttgtt 420 gtttgcgaca gttaaataaa acttaacgac aaagaagtga cttgcgtcgt agattaactg 480 gaaattaaag tgg 493 <210> 209 <211> 133 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 209 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt attagcctgc ctaattgttg gtaacaatgt aatggaaatt 120 ggcgatgact atg 133 <210> 210 <211> 82 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 210 attgtaagtt taatttacca tttcgtcatt gtatatgcgg taaacgtaaa tttacacata 60 tgacacgaac tttgagtttt at 82 <210> 211 <211> 276 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 211 gctctcatgt agcctacctt acgaagaaat cgcacaagtc ggtcgcccag cggtggcata 60 tgtcttgcct aacgtttcta gaccgatcag cctcacgagt agcctgcttg tggtgtttat 120 agtttacgct agtccaatct gtatcgtgcc gcttggtatg gctattgtcg gcctggatgc 180 aagcactaac gtggtcatca gactggtctg cgctataatc tacgcaatag aggcaccagc 240 ggtcgttgaa gccgaagacg ctatttcggt ggcttg 276 <210> 212 <211> 182 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 212 gctctcatgt agcctacctt acgaagaaat cgcacaagtc ggtcgcccag cggtggcata 60 tgtcttgcct aacgtttcta gaccgatcag cctcacgagt agcctgcttg tggtgtttat 120 agtttacgct agtccaatct gtatcgtgcc gcttggtatg gctattgtcg gcctggatgc 180 aa 182 <210> 213 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 213 aagaagcgaa acggcattcg tttaacaaaa cgtcacatat ttaggtatag tagattgcgt 60 tcacaaagaa atttaaagtc ttgtaattat ggtggtcgat ttattggtag tatatttgga 120 aatagcatgc aattttggaa tggacgaaga acctgacatc tgagctattg taagtttaat 180 ttaccatttc gtcattgtat atgcggtaaa cgtaaattta cacatatgac acgaactttg 240 agttttatta gcctgcctaa ttgttggtaa caatgtaatg gaaattggcg atgactatgg 300 <210> 214 <211> 77 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 214 aagaagcgaa acggcattcg tttaacaaaa cgtcacatat ttaggtatag tagattgcgt 60 tcacaaagaa atttaaa 77 <210> 215 <211> 84 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 215 aaataagaag aagcgaaacg gcattcgttt aacaaaacgt cacatattta ggtatagtag 60 attgcgttca caaagaaatt taaa 84 <210> 216 <211> 132 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 216 gaacttaaat tgttagagca gtttatgggt ggtaggttac atctatttac ttcaaaacgc 60 ggtgccctag cccacaagca ttgctttgta aaattgttga aggtaacttg taaaagccga 120 acatgaacga tg 132 <210> 217 <211> 66 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 217 gtatatgtac tttggtaacg ctctaaggta ggtacacgtt agtgatataa aaagaaggcg 60 acgacg 66 <210> 218 <211> 104 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 218 agattgcaga gagccaggtc aaatacattg agaaatctgt atatgtactt tggtaacgct 60 ctaaggtagg tacacgttag tgatataaaa agaaggcgac gacg 104 <210> 219 <211> 384 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 219 gtcacctaaa cgtgcgaagt gtgcgaaatt accaccgtaa attcgtccta ctgaaagtgc 60 ttacgcatct tgagagagat tgcagagagc caggtcaaat acattgagaa atctgtatat 120 gtactttggt aacgctctaa ggtaggtaca cgttagtgat ataaaaagaa ggcgacgacg 180 gtagcagagg tctagaaacc gcatgaacga ccaacttatt tgaagagcta gtgcggtgga 240 attgggtcgg aagaacttaa attgttagag cagtttatgg gtggtaggtt acatctattt 300 acttcaaaac gcggtgccct agcccacaag cattgctttg taaaattgtt gaaggtaact 360 tgtaaaagcc gaacatgaac gatg 384 <210> 220 <211> 511 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 220 gatgtggaag gtcacagcct cgttggagga gatactacgg tcccgctttt cgtgggtgac 60 aaaggtcgca aaggtttcct tagggaggtc gtttcccgga tctttatcta catctcctgg 120 gtgaagggga tgatctttct ctacatgtcc tgggtggagg ggagtttcct tgtatgggtg 180 gaacccaacc cgccggtgag ggtgagttac ggtcggcggt catatgtggg tatgacgggt 240 atgacgtcat aaatagtcgt tgtcaaaatg ggaggtggaa gtccttactg tagtcacccg 300 tgaaggtgga gaactggcag gacatctcta ggtcacctaa acgtgcgaag tgtgcgaaat 360 taccaccgta aattcgtcct actgaaagtg cttacgcatc ttgagagaga ttgcagagag 420 ccaggtcaaa tacattgaga aatctgtata tgtactttgg taacgctcta aggtaggtac 480 acgttagtga tataaaaaga aggcgacgac g 511 <210> 221 <211> 146 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 221 gatgtggaag gtcacagcct cgttggagga gatactacgg tcccgctttt cgtgggtgac 60 aaaggtcgca aaggtttcct tagggaggtc gtttcccgga tctttatcta catctcctgg 120 gtgaagggga tgatctttct ctacat 146 <210> 222 <211> 81 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 222 gagaagatga caaagtagaa gctagacaac tatatcaaat cgttgtgtgg tcttaaaccg 60 acacagtagc attcatcgtt g 81 <210> 223 <211> 163 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 223 gagaaacttg tctactggca gacctcacta tcttttagga aggtcacgaa gcggctggag 60 tgatggctag tctcagccac tgtcaacggg aagcgcgacg tttatggtca atataaacta 120 gcagtttaat ccgtatggcg atcttagcgg atgttacagg atg 163 <210> 224 <211> 131 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 224 gagtgttgta ttaattcact ctagagtaat ggatcacggt gccagaaact aaggtggtgt 60 aggactagat gcaagtaaca acggtcgtac taaaatcagc tacgcgatcg ccgacttaga 120 aggctgagat g 131 <210> 225 <211> 64 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 225 gatgtgaaaa tgtcatatgt gcggcaaaaa tagacgacat ttaagaacct agcattatag 60 gatg 64 <210> 226 <211> 125 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 226 gaacacagat attgcagaag cgggaatgag gttgatatta catcaagtat caacagacga 60 cctcagaagg gtggtctttt aaaatgacaa cgtacattaa gatgcagaca aacactacta 120 tgctg 125 <210> 227 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 227 gattacgacg ccaagccact ataaatgcta attgagtcga agaaggtact cctttttg 58 <210> 228 <211> 83 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 228 gatctaagat gactacagta gcatagccat agaacatggc actgaaatat cgatgggcgt 60 aaaagtgaac aacgcagacg atg 83 <210> 229 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 229 gaaatcgcat cgacacaata aaagtacacc aaccagcaga aaacaggtac atatacagtt 60 taacgacctg 70 <210> 230 <211> 160 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 230 gaaccaagca gtgggcgatg tcagcgacaa ttccactttg gtagaggtcg ttcttttgta 60 gaaaacaatc taacataact atgacacttt cggtaaattc accaccttta ccagggtcgt 120 aagtggtcaa gccacgccga ccacttcata agcattggtg 160 <210> 231 <211> 115 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 231 gaaccaagca gtgggcgatg tcagcgacaa ttccactttg gtagaggtcg ttcttttgta 60 gaaaacaatc taacataact atgacacttt cggtaaattc accaccttta ccagg 115 <210> 232 <211> 106 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 232 gatcgtttgc ccgtaaaact aactggcaaa agcctaagtg ctgtttcggg tgcaatggta 60 taacttccca catttagccg aatagcgcca acagcgttat tatttg 106 <210> 233 <211> 93 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 233 gtagaccttt ccttgccgcg tggaacaaac cgccatgagt gttttacgtt tacggtcggc 60 gaccggagtc acagcgcgac caacggggcc agg 93 <210> 234 <211> 121 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 234 gcccatgaat tacccgctta ggcaatgggt agacctttcc ttgccgcgtg gaacaaaccg 60 ccatgagtgt tttacgttta cggtcggcga ccggagtcac agcgcgacca acggggccag 120 g 121 <210> 235 <211> 122 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 235 ggcccatgaa ttacccgctt aggcaatggg tagacctttc cttgccgcgt ggaacaaacc 60 gccatgagtg ttttacgttt acggtcggcg accggagtca cagcgcgacc aacggggcca 120 gg 122 <210> 236 <211> 130 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 236 gacttgtagg cccatgaatt acccgcttag gcaatgggta gacctttcct tgccgcgtgg 60 aacaaaccgc catgagtgtt ttacgtttac ggtcggcgac cggagtcaca gcgcgaccaa 120 cggggccagg 130 <210> 237 <211> 141 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 237 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggggccag g 141 <210> 238 <211> 135 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 238 gcatactgca agacttgtag gcccatgaat tacccgctta ggcaatgggt agacctttcc 60 ttgccgcgtg gaacaaaccg ccatgagtgt tttacgttta cggtcggcga ccggagtcac 120 agcgcgacca acggg 135 <210> 239 <211> 218 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 239 gagcgttagt cggcgcgtgc gcgggactat ttagcgaagg tcgccgtatc gcgggtggcg 60 gcgtttccgc cataacaaca agcgtttaag gtcagagtcc gcgacgaatg aaacgccgtg 120 caagcggctt tggtacggcc agtttagcca aagcctgatc cgttcgcctt gggttatcat 180 gtaccagtca agcggtcatc gcgggcggcg ttatcatg 218 <210> 240 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 240 gagcgttagt cggcgcgtgc gcgggactat ttagcgaagg tcgccgtatc g 51 <210> 241 <211> 229 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 241 actttccagt tagtcgccgt cctatattgg caagaccgga tggccgtcaa ccagttccat 60 tctgactatt tgccacattg attaaagaag taggagcgtt agtcggcgcg tgcgcgggac 120 tatttagcga aggtcgccgt atcgcgggtg gcggcgtttc cgccataaca acaagcgttt 180 aaggtcagag tccgcgacga atgaaacgcc gtgcaagcgg ctttggtac 229 <210> 242 <211> 202 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 242 gtgttaggtg caatccccat tcgggacgga cacgcgagta aaggtctaac atgtttcaac 60 tttccagtta gtcgccgtcc tatattggca agaccggatg gccgtcaacc agttccattc 120 tgactatttg ccacattgat taaagaagta ggagcgttag tcggcgcgtg cgcgggacta 180 tttagcgaag gtcgccgtat cg 202 <210> 243 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 243 gtgttaggtg caatccccat tcgggacgga cacgcgag 38 <210> 244 <211> 412 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 244 gcaaaggccc ataggctttt ccatcggcac gcgaccgatt ccatcaagta tagccacggt 60 cgtaacttcg acggcggcaa tagtttatgg cgctaacacc tttccatcgc tgagtccaaa 120 cgtatgacgt gaggtgttag gtgcaatccc cattcgggac ggacacgcga gtaaaggtct 180 aacatgtttc aactttccag ttagtcgccg tcctatattg gcaagaccgg atggccgtca 240 accagttcca ttctgactat ttgccacatt gattaaagaa gtaggagcgt tagtcggcgc 300 gtgcgcggga ctatttagcg aaggtcgccg tatcgcgggt ggcggcgttt ccgccataac 360 aacaagcgtt taaggtcaga gtccgcgacg aatgaaacgc cgtgcaagcg gc 412 <210> 245 <211> 417 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 245 ccagagcaaa ggcccatagg cttttccatc ggcacgcgac cgattccatc aagtatagcc 60 acggtcgtaa cttcgacggc ggcaatagtt tatggcgcta acacctttcc atcgctgagt 120 ccaaacgtat gacgtgaggt gttaggtgca atccccattc gggacggaca cgcgagtaaa 180 ggtctaacat gtttcaactt tccagttagt cgccgtccta tattggcaag accggatggc 240 cgtcaaccag ttccattctg actatttgcc acattgatta aagaagtagg agcgttagtc 300 ggcgcgtgcg cgggactatt tagcgaaggt cgccgtatcg cgggtggcgg cgtttccgcc 360 ataacaacaa gcgtttaagg tcagagtccg cgacgaatga aacgccgtgc aagcggc 417 <210> 246 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 246 ccagagcaaa ggcccatagg cttttccatc ggcacgcgac cgattccatc aagt 54 <210> 247 <211> 110 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 247 gaggaagcct tttagccatc gctatgccgc cgctcaaacg cgccgggtgc aaaagcccag 60 agcaaaggcc cataggcttt tccatcggca cgcgaccgat tccatcaagt 110 <210> 248 <211> 557 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 248 agctgctcgc atgaccagct atatctggaa catcgctggt gggtcttatg gtcgcttttg 60 tgggaaccat catagtcgca agtctggcct tcggtatgga actcgtcgtc ccgactacaa 120 ccatagctag tctcggtctc ggctttgttg caatgccctt cgttactact tcaacggtcg 180 tggtgccggt caaaccgctc gctaaagcaa gcgaagaaac agctaaactt agttgtgtcg 240 aaacatccta agtggtcggg ttgctcgtgg agcatgcagc tagcccagaa cctttagctt 300 gtgtggtctt cgtctcggta gtcagaacca ggtcggtctg aatggttgga ctcgccacat 360 caactaggtc agctattgaa gtgccaacta aaggtcgtat tgaagcaatc ggtcctatgg 420 caagtcaagt gacagtcgta ggtatggcac ttgccgcgcc tccagaactc ggtcaagcta 480 cctcataact cggaagtcga ctgctctaaa gcgaagttgt caaacgaaca gctatgcctt 540 cgagtaaagc aaggtgc 557 <210> 249 <211> 2473 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 249 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 60 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 120 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 180 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 240 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 300 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 360 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 420 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 480 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 540 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 600 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 660 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 720 taaagcaagg tgcgcaatag taaccttggt aacttcggaa ctgcgtcaag ccgctaactc 780 catcggtatg gaagtcgcta aagtcggtca aacggaagcg gctatcgtcg caactagtcg 840 aatagttgtc gaagaggcca gagcaaccat ggttgccatt gtcttcgtgg tctatgcaag 900 acgtccttgc gtcatggcct aagcatcttc ttcactggtt gctatagcgg aagtcgttac 960 cgaactggtc gccagtcgaa tcgtttgccc catggtagtc atcaccacca tcgcggaaac 1020 caacggtccg aactcacggt aggtagtcca cgtatgtagc ccacctttcg aaggaatcgt 1080 ggtcgccggt ctcctagaaa cggtatatgt cttgcgtcac gcttatggac cggtcatcct 1140 ggccagtagc cgacttgtgg tcgctatagc tttcgctagt ccctgcctta acgtgcagca 1200 tggtatggct attgtcgtcc cggctacaac cactatcgtg gtcttgaaac tggtcgtcgc 1260 tacaaccatc ctagcattgg caccagcggt cgttgaagcc gaaggtagtc tctaactggt 1320 tttcgaagct agtcaaacag tagcgccata tggcctagcc tgaaccttag aaaaaggagt 1380 cgaaggtcct aaacccgctg gtcgcgaacg aacaaaaacg gtcgttaaag gccgtccgga 1440 agcggaaggt cgtcgcccca ggtgaacaag tcctaaagaa acaaaaagtc tctgtagtct 1500 atttaaaacc caacgcaaac gcgaacgcag aatcgtctga acccacgtcc atcattacgg 1560 attatataag gtttctgttt cgttaactag gtagttattg atgtcatccg cactacaagc 1620 taacaggagg taacagatag agcatgagtg gtagtaggga tgaaacagga agtactaatt 1680 acatacaggg actcctggtt gggtgacccc gggacacgta cgtgtcctaa taaacaggag 1740 gcgggtggtt tcctatctac tacggacagg gattttcccc gagtagcgaa ggatatgaaa 1800 cttctagtta tagacatata agacgtagtc caggaagtcg gctgagtcca tgaggtcagt 1860 tttggaggag aagtagttgt cacgatcctt agttaagata acaaagtccg agtagttatc 1920 acgacttgct gactgcgtcc actatgtttc cggggtgggc atggggactt gcacacggat 1980 aactccatcc ggattattaa gacgaggtgg ggtgtgctgg gctcccagga acttcatcgg 2040 agtgacgtag aaccttcagt ttgagtccac gtaggacacc agcgctattg gtgccgacga 2100 cgagtacaaa catccggaaa cttagcatat taggtaagag tttctgtatg ctcggacgtc 2160 gtcgttagag cagacccaga aggtgtccat ggacacacat catacaagac aacttggtca 2220 acttttacgg ggctggggcc aattatcccg ggagtcctca cgatcagaaa ccgactggtt 2280 tgtcacactc tggttagagg aggggtcacc acgatgggtg agggggtggt gggtgtttat 2340 acaagagtct gggactccaa agacgatatt acgacgagac gacttatcca cgtactttgt 2400 ttcccaaacc cacgcaactc cacctttagt ccacacaaac tagtacgaga cacccggatt 2460 caaggtcgtg acc 2473 <210> 250 <211> 734 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 250 gtccacgctc taagtcgctt gcccaattgt ggctaccgca ttgctcagaa cttcgggtac 60 tggccaacct tcaagaagcc tagtcttggt gcgtcacccc agtcacgtag catacccgtc 120 atcatctatg gtcgtaggtc caacaatcgt ggcgccattg ggtaccactt catcagagct 180 gctcgcatga ccagctatat ctggaacatc gctggtgggt cttatggtcg cttttgtggg 240 aaccatcata gtcgcaagtc tggccttcgg tatggaactc gtcgtcccga ctacaaccat 300 agctagtctc ggtctcggct ttgttgcaat gcccttcgtt actacttcaa cggtcgtggt 360 gccggtcaaa ccgctcgcta aagcaagcga agaaacagct aaacttagtt gtgtcgaaac 420 atcctaagtg gtcgggttgc tcgtggagca tgcagctagc ccagaacctt tagcttgtgt 480 ggtcttcgtc tcggtagtca gaaccaggtc ggtctgaatg gttggactcg ccacatcaac 540 taggtcagct attgaagtgc caactaaagg tcgtattgaa gcaatcggtc ctatggcaag 600 tcaagtgaca gtcgtaggta tggcacttgc cgcgcctcca gaactcggtc aagctacctc 660 ataactcgga agtcgactgc tctaaagcga agttgtcaaa cgaacagcta tgccttcgag 720 taaagcaagg tgcg 734 <210> 251 <211> 133 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 251 gatagtagac cgggaagacc aacgtttttt cactactccg aaagaacaca ctgaagggaa 60 cggtgtaggt tcggtgagtc cgggactagg tgtccatgtc ggagacctca gtggtgacgt 120 cctcgtagta tag 133 <210> 252 <211> 132 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 252 gatagtagac cgggaagacc aacgtttttt cactactccg aaagaacaca ctgaagggaa 60 cggtgtaggt tcggtgagtc cgggactagg tgtccatgtc ggagacctca gtggtgacgt 120 cctcgtagta ta 132 <210> 253 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 253 caaacgatgt cttgaacaac accgtcttca ttgtttacca tttgctgttg 50 <210> 254 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 254 caaacgatgt cttgaacaac accgtcttca ttgtttacca tttgctgtt 49 <210> 255 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 255 gcaattagga tcaggtggtt aatctgatac g 31 <210> 256 <211> 118 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 256 ggaggacatg cacctagttg aacacgacta ggaggacgat ggccactacg gagtggaaca 60 caaaggtaaa gttgcaataa taaccagtac aacaaaagca tacgtggaag gtgacaag 118 <210> 257 <211> 361 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 257 gaatgtatgt aattattcgc aagggttcca taaggcacat atacaaattt agaaacaacc 60 cgttacatta acaaaggtga acggtgtttc acagatatta gaccattagg taaccaatga 120 catttcacta tcgaaaaggg tatagtatta actactacaa ctaatccgac tcgtcgaggt 180 gatttataca ggactgcagg tagcaaacga cgaggtggac gagggtaagg tggccgacga 240 aagggaggac atgcacctag ttgaacacga ctaggaggac gatggccact acggagtgga 300 acacaaaggt aaagttgcaa taataaccag tacaacaaaa gcatacgtgg aaggtgacaa 360 g 361 <210> 258 <211> 145 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 258 gaatgtatgt aattattcgc aagggttcca taaggcacat atacaaattt agaaacaacc 60 cgttacatta acaaaggtga acggtgtttc acagatatta gaccattagg taaccaatga 120 catttcacta tcgaaaaggg tatag 145 <210> 259 <211> 110 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 259 gtgaaaggtg atacatattt acttgacttc ttaagcagag agcgtcaaaa caggttgaac 60 ttatacagaa atactaaaag tcgaacaacc attcgattta ggaatacaga 110 <210> 260 <211> 151 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 260 gatacagaac aactaaaaga actaaaagag aagaacgacg tgcacgtgct aattatgcga 60 attaacatcg ggttagaaaa agtaaactaa aggaaaatgg gtaaggcata tgccttacta 120 aattgtgtaa aagaagttct aataagaacc g 151 <210> 261 <211> 156 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 261 gtgcagatac agaacaacta aaagaactaa aagagaagaa cgacgtgcac gtgctaatta 60 tgcgaattaa catcgggtta gaaaaagtaa actaaaggaa aatgggtaag gcatatgcct 120 tactaaattg tgtaaaagaa gttctaataa gaaccg 156 <210> 262 <211> 346 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 262 caccaccgga gggttgctag accctaacgt gggagacgct ctatccacag ctacatcatt 60 cagaagagaa agaagagggt cttctacagg ggacattgca gctaacacgc tggttgttcg 120 ttgcgtagac gacacttgtt ctgtcgctac cgaggcaagt aaagtcgcca acgtagtatc 180 gccaatggta acagtagcaa tctgtcattc accacgcgaa ctggactatg tttaaatccc 240 tttgccaatc ttccctattg tcccgtcaac ttccttctgt cttgctttgg taagtgtcaa 300 cgtggcctga ccgaatatca cattcagaac agttgctatg ctacac 346 <210> 263 <211> 265 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 263 gccaaggtga accaggacga agtcgatgtt tcagaacctc ctttaggcct tttagacggt 60 ttaaacgaag cactagacca agtaataaaa gcggacgttg cacaggtacg tgcagataca 120 gaacaactaa aagaactaaa agagaagaac gacgtgcacg tgctaattat gcgaattaac 180 atcgggttag aaaaagtaaa ctaaaggaaa atgggtaagg catatgcctt actaaattgt 240 gtaaaagaag ttctaataag aaccg 265 <210> 264 <211> 430 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 264 ctagcgcgtc cgtcaacttc acactggttc cgtagtccta acgttgtagt ctgtgcagat 60 gaagaacgag gtcaacaacg aggtcacgta tgagataccg tcggtagtcc atgacacccg 120 aaaggtcacg tcaactaagc aattgaagtt gcaattacct cttcggtact ggaaattggg 180 cacccaacca agcgaaccga aaccacactc gttacggtgg ctcgtcgttg acgacctgct 240 ggtaaagcag aaggtacgcg aagttaacga accagaccaa gtatcggtcg aatccgaatt 300 gtcttagtta cagtgggact tgcagtacga cgtatcgttg ccacaatgcc cgttattgtc 360 accacagcta atggtgaacc aagaacagcc agactcatgg tcaacgtaat ccactgactg 420 ggccacgatc 430 <210> 265 <211> 208 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 265 ctagcgcgtc cgtcaacttc acactggttc cgtagtccta acgttgtagt ctgtgcagat 60 gaagaacgag gtcaacaacg aggtcacgta tgagataccg tcggtagtcc atgacacccg 120 aaaggtcacg tcaactaagc aattgaagtt gcaattacct cttcggtact ggaaattggg 180 cacccaacca agcgaaccga aaccacac 208 <210> 266 <211> 520 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 266 gacggttaaa gttattgact tatgttagca tgtgcaaatt aacataggct agtcttaacc 60 tgactcgctg tcgcgactgg catgcaaagt ttgacacgcc gcggctctag tacgcgtcat 120 ctttcagctg taataactac aaacacatca aaaaaagtcg gtttaaaaca acttgtgaaa 180 cgtcaacttg gtaaataaac ctattattaa agaatctaga tcaatgtcca aaaagattat 240 ttccattgtt acgtctaaat acagattctg cgaaaacaag ccattagtat agtagaagaa 300 ctaaccatcg tatgttcgtg aacgtcgagt caaagtggct acatgaacaa cgattgccac 360 aagcatggtc agtgtcagac ttctagcttc atcgggtcgt ctaagagtgg aaagtccatg 420 acgagcaatt ggaatggcaa tttgaagagg tactcttccg acagttggag tggtctccgc 480 ctttgccgtt gtaaccaatc caataaaccg catctgtaaa 520 <210> 267 <211> 1019 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 267 cctgtttcta gcatgtataa caaaattaag cgacgaccga gcgacgacaa caagcagcag 60 ccaggtgggc aaaaggctaa gactgctttg atgtcctgag ccaacttgcc gatacgcagc 120 aacattttag aaacgtcatc ctgaccgccg ccgccattgc cataaatgat agcttggcgg 180 tagccgccca aaactatgaa aaaaattaaa ttaaggaaag acataaacaa ggtagttaag 240 ccatgcacct agaaaatttt gaacggctca gctgcaacat attagcgcta gaaatgacct 300 accataatga tatagaagat aattaccatt actgccaccg cctcctccgc tgccgcctcc 360 tccatagcag cttctattca aacaaactcc gccgccgcca ccgccgccat aaccaccacc 420 ataaccaccg ccgtatacac aaacgccgct ccttctaagt cttagctatt aataacaacc 480 gtggaaacaa aaaacgtaat ataggctaca gctgtgtcaa cagccaaatc cataacaaca 540 aaatccatgg tgactgtaac agacactgtt acaaccatat tattaactag atagtggtta 600 cagataatca tgcagcaaca acatataaag aagacggtta aagttattga cttatgttag 660 catgtgcaaa ttaacatagg ctagtcttaa cctgactcgc tgtcgcgact ggcatgcaaa 720 gtttgacacg ccgcggctct agtacgcgtc atctttcagc tgtaataact acaaacacat 780 caaaaaaagt cggtttaaaa caacttgtga aacgtcaact tggtaaataa acctattatt 840 aaagaatcta gatcaatgtc caaaaagatt atttccattg ttacgtctaa atacagattc 900 tgcgaaaaca agccattagt atagtagaag aactaaccat cgtatgttcg tgaacgtcga 960 gtcaaagtgg ctacatgaac aacgattgcc acaagcatgg tcagtgtcag acttctagc 1019 <210> 268 <211> 468 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 268 cttgttccaa tcggcccggc ttcagtcatt cccggtggcg aagccatgtc accaataaaa 60 actatcggcg gcaatggaag tggtttttcg gaaactgtta cgggtgcaag cggccccagt 120 gcagtttgtg ccttgccaat cggcccagct ttcaaacgca aagttggacg acttattggc 180 ccattctgac aacgtcctgt taaactgccc ggacgttcaa acggcggtat cggacgacta 240 acgcgttacg gcggacaact cgcccccagt caacaacttg gacacggtaa atttgaacgc 300 taagtcaact gtagcagtta aacattgagt attaaaatag atttaagcta gcgttaagaa 360 cattaaaaac taaccagcca aaccaaggat tacggctgtg gtgtaatcga ttgcgaaata 420 gcatgacaaa aaacttacag tttagaaggt ggcggtatta cttaagaa 468 <210> 269 <211> 151 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 269 gacatcagat gttaatgacc taacgattat agtgctgaac aacgtctacg acaatataaa 60 cctttgcaaa gactggtcaa acatcaatgt taaacacgtc cagataaaag caaaaaggta 120 agacgaggat tacggaataa ttaatatagt g 151 <210> 270 <211> 247 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 270 gaaacaagct atgctaatgt tgaccatttg gctcttcgac cagcagtcag ccgcttcttt 60 acctgccaac gttgttcata acaggcaaaa ccagaaagtc gcataggttg gtcaactttc 120 gcagaaccaa ctgttactgc gatcttcagc ttgccttttg tcccagatta acgtgtaatt 180 attaaacaaa gttcggcagt tccagtcgct atcgacaaaa ccgaaaccac aagttctgta 240 attacgc 247 <210> 271 <211> 509 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 271 cattcgttca acatgaagtg gcttatgttc gcggagtagt agcgcgtaaa agtgtaggta 60 cgcgagctat cgtgcggtgt agcatcgcag tcaaagcttg cgtaggtagc gttgcaatct 120 cctgtactaa agacatgcaa ctgccagctt gtactgttgg cgaagaaagt tcagtggatg 180 ttactaaccg cgtttaacgc gtggacacgg cggcagttga gccaatcgat tacgaaacaa 240 gctatgctaa tgttgaccat ttggctcttc gaccagcagt cagccgcttc tttacctgcc 300 aacgttgttc ataacaggca aaaccagaaa gtcgcatagg ttggtcaact ttcgcagaac 360 caactgttac tgcgatcttc agcttgcctt ttgtcccaga ttaacgtgta attattaaac 420 aaagttcggc agttccagtc gctatcgaca aaaccgaaac cacaagttct gtaattacgc 480 gttcggcaaa gcgcaatgcg ccctaatac 509 <210> 272 <211> 135 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 272 gtggttaggt cgcttggtag catatctgtt accgaagaca cgacagtaaa ttgtcaagca 60 aacactgcgc cttcttcggt cgcataaaga agtgaaccat aacgttctcc tacttaactc 120 cgcaacgaaa aagag 135 <210> 273 <211> 110 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 273 gtggttaggt cgcttggtag catatctgtt accgaagaca cgacagtaaa ttgtcaagca 60 aacactgcgc cttcttcggt cgcataaaga agtgaaccat aacgttctcc 110 <210> 274 <211> 178 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 274 acatttgatt cgttgacgta tttatgttga ttcggttagc gagagttaca aagttagtaa 60 tagaaagaaa gaggaaataa taatcaaaag attaagcatg caaacattaa gaggacacat 120 atgtattggt ggcatttgcg ttaggttaac gtctcgtaag acggtgaagc tgcttaaa 178 <210> 275 <211> 216 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 275 ttacgatccg aaatttcatg gacacagata tttgagatca acatttgatt cgttgacgta 60 tttatgttga ttcggttagc gagagttaca aagttagtaa tagaaagaaa gaggaaataa 120 taatcaaaag attaagcatg caaacattaa gaggacacat atgtattggt ggcatttgcg 180 ttaggttaac gtctcgtaag acggtgaagc tgctta 216 <210> 276 <211> 185 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 276 gattacgatc cgaaatttca tggacacaga tatttgagat caacatttga ttcgttgacg 60 tatttatgtt gattcggtta gcgagagtta caaagttagt aatagaaaga aagaggaaat 120 aataatcaaa agattaagca tgcaaacatt aagaggacac atatgtattg gtggcatttg 180 cgtta 185 <210> 277 <211> 182 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 277 gattacgatc cgaaatttca tggacacaga tatttgagat caacatttga ttcgttgacg 60 tatttatgtt gattcggtta gcgagagtta caaagttagt aatagaaaga aagaggaaat 120 aataatcaaa agattaagca tgcaaacatt aagaggacac atatgtattg gtggcatttg 180 cg 182 <210> 278 <211> 92 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 278 aaagaagatg cacttagtgc cattattatt agacaagata gtccgttctt tagtcgaagt 60 tcttgttgta ataacaaaag tagagcagta tt 92 <210> 279 <211> 232 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 279 gtttcaacaa tgcccaagtt gacattattc caaaacgttt cgtagaaatg aaagcgcttc 60 gagtaattag gcgcaaactt acaggacgcc atggcatatg ggtggtagaa ccaacgatgg 120 aagcgagtag cgcaatgcct tagcgcacgc ctacactaac tgcttacgaa gttggagcaa 180 aagaaactga acgtgtcagc caaaattgcc aagaaacagg agctaccaaa ag 232 <210> 280 <211> 116 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 280 catcgtacga actttgacaa cgaaaggttc aaaggtaacc aaggcgtagt agtgagttag 60 gtcaatgtat aaaaactccc tttcttattt tgagtcgtca atgtagtcga gaaagt 116 <210> 281 <211> 96 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 281 tattgttata ggcggtatat tggtaaatga cgatccttga cttagttttt tacatcgaac 60 ggttgcaaat cctagcatac ttgatattaa tagtag 96 <210> 282 <211> 2235 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 282 gagtaaacga acatccaaca gtatgtacaa tcgtatgaga aaggacaaat ctgaagacga 60 tattagaggg agtccaacaa gtttgtgatg attcacgtaa ggaaatcgaa atgtaagatt 120 attcgtcaat ggaggtataa gacaacacac caagtaatta tacgcgacga gttttcatgt 180 tgacgatccc tgagactata tcatacaaaa ttgacccaaa taataaactc gttccaatag 240 ttgaagtgtt gattaacaca ttgagacaag ttgtccgtac tttaatcatc aaagttttgg 300 actgacctat aaatgaaata ttcgcgttgt cttcagttta agaagtgata ttattttcgg 360 tttcaagcct atgtggagag atacaggaaa taggtaaact ataactaata taaatatacg 420 cagacgaagc ctgttaactt taccaaatcc aaatagcggg agacactaaa cttacaagaa 480 atgtacggta agacagggaa acagggtttg aagtttggag gtacaaaatc ttaatccttc 540 acaactgaat caacatttac attcggttac gttcggggtt tgtagtagta attgttgagg 600 taattttaat tgcacttaca gacgtggatt tggtgaaaca ttatgtggta gattcatatt 660 tccgaatctt aatagacgat tttatgatgg tgatcgtggt ttcttttcaa ggtttggtgg 720 acgaaaatga aaaaatcaca atttaacgtt tcatattcat cacaatccga aatcgagatg 780 gagagaaagt ttgactaaac tttgttacag tatttttgaa ggcaatcaac ctggaacttt 840 ggatttcatc aagtaaacac ccatatttag cgaaaatgca agctaactgc gaactaacaa 900 ggtaactctt tttcgatttc aaaatatggt cagtagcaat atgttaagtg atccattttg 960 gaggggtggg gatttgttca atgaatgagg caataaacca ccgcaacctc tgtaattgac 1020 gagaaacttg tataaggtca tcaaaaagaa tagatgaaca taaagattga atacggctgg 1080 gtgaggttaa ttcagatttc ccaaattatg tagatgaaaa gatggtaagt ttattgatag 1140 gtaaaaaatc aagagagggt aataaagaag aaagaataat caaaagatag agcatgcaaa 1200 cattacatgg tagtattgtt ataggcggta tattggtaaa tgacgatcct tgacttagtt 1260 ttttacatcg aacggttgca aatcctagca tacttgatat taatagtaga tactgaaata 1320 tatctgagta tatcaaggga aatcgagatg taacaataag tcgaggaaat taaggtcaat 1380 ttagtgctta tcatagaaat aactacttaa gcccttaacg aactaactca aattaacgag 1440 gtagaggtta ttctgaattg tacgaacgta gtcgaagttt gttaccaagg gtaaagccaa 1500 aacaaaacga tttgacaaga gaatctattc catgtagtag tgcgtttgta cgacgttcta 1560 ttcgatgtaa atcattagga cgagggtttg aagcaactcg gaaagatggt cggttagaat 1620 ttaaaagtac tgaacggaat taacgaagtt ggctactacc taatagtctt tgcttaggat 1680 ttagtagttc gcaattgggt caaagtggtc gctttggtcg tcgacgtcaa gactcaacaa 1740 gtcatttacc gctgtacgaa ccatctcatt taacaggttc tcaaccaaat agcggtagga 1800 gtagaaagca cttaccatcg tacgaacttt gacaacgaaa ggttcaaagg taaccaaggc 1860 gtagtagtga gttaggtcaa tgtataaaaa ctccctttct tattttgagt cgtcaatgta 1920 gtcgagaaag tgtccaagca aaacgtaacg aaacggttga gtaaggactt gcacactatg 1980 cccttgcggg tttacctgct tgacgtagtc ctgccactta ttatccgtga ttgttaccta 2040 atggacaacc taatcgttag cttaaagaag atgcacttag tgccattatt attagacaag 2100 atagtccgtt ctttagtcga agttcttgtt gtaataacaa aagtagagca gtattttgac 2160 gtatagggag acagtcatta ggtcattaac ccagttacta aggacaacgt aaccatcgaa 2220 gttattgaac ttcag 2235 <210> 283 <211> 1765 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 283 gagtaaacga acatccaaca gtatgtacaa tcgtatgaga aaggacaaat ctgaagacga 60 tattagaggg agtccaacaa gtttgtgatg attcacgtaa ggaaatcgaa atgtaagatt 120 attcgtcaat ggaggtataa gacaacacac caagtaatta tacgcgacga gttttcatgt 180 tgacgatccc tgagactata tcatacaaaa ttgacccaaa taataaactc gttccaatag 240 ttgaagtgtt gattaacaca ttgagacaag ttgtccgtac tttaatcatc aaagttttgg 300 actgacctat aaatgaaata ttcgcgttgt cttcagttta agaagtgata ttattttcgg 360 tttcaagcct atgtggagag atacaggaaa taggtaaact ataactaata taaatatacg 420 cagacgaagc ctgttaactt taccaaatcc aaatagcggg agacactaaa cttacaagaa 480 atgtacggta agacagggaa acagggtttg aagtttggag gtacaaaatc ttaatccttc 540 acaactgaat caacatttac attcggttac gttcggggtt tgtagtagta attgttgagg 600 taattttaat tgcacttaca gacgtggatt tggtgaaaca ttatgtggta gattcatatt 660 tccgaatctt aatagacgat tttatgatgg tgatcgtggt ttcttttcaa ggtttggtgg 720 acgaaaatga aaaaatcaca atttaacgtt tcatattcat cacaatccga aatcgagatg 780 gagagaaagt ttgactaaac tttgttacag tatttttgaa ggcaatcaac ctggaacttt 840 ggatttcatc aagtaaacac ccatatttag cgaaaatgca agctaactgc gaactaacaa 900 ggtaactctt tttcgatttc aaaatatggt cagtagcaat atgttaagtg atccattttg 960 gaggggtggg gatttgttca atgaatgagg caataaacca ccgcaacctc tgtaattgac 1020 gagaaacttg tataaggtca tcaaaaagaa tagatgaaca taaagattga atacggctgg 1080 gtgaggttaa ttcagatttc ccaaattatg tagatgaaaa gatggtaagt ttattgatag 1140 gtaaaaaatc aagagagggt aataaagaag aaagaataat caaaagatag agcatgcaaa 1200 cattacatgg tagtattgtt ataggcggta tattggtaaa tgacgatcct tgacttagtt 1260 ttttacatcg aacggttgca aatcctagca tacttgatat taatagtaga tactgaaata 1320 tatctgagta tatcaaggga aatcgagatg taacaataag tcgaggaaat taaggtcaat 1380 ttagtgctta tcatagaaat aactacttaa gcccttaacg aactaactca aattaacgag 1440 gtagaggtta ttctgaattg tacgaacgta gtcgaagttt gttaccaagg gtaaagccaa 1500 aacaaaacga tttgacaaga gaatctattc catgtagtag tgcgtttgta cgacgttcta 1560 ttcgatgtaa atcattagga cgagggtttg aagcaactcg gaaagatggt cggttagaat 1620 ttaaaagtac tgaacggaat taacgaagtt ggctactacc taatagtctt tgcttaggat 1680 ttagtagttc gcaattgggt caaagtggtc gctttggtcg tcgacgtcaa gactcaacaa 1740 gtcatttacc gctgtacgaa ccatc 1765 <210> 284 <211> 277 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 284 gagtaaacga acatccaaca gtatgtacaa tcgtatgaga aaggacaaat ctgaagacga 60 tattagaggg agtccaacaa gtttgtgatg attcacgtaa ggaaatcgaa atgtaagatt 120 attcgtcaat ggaggtataa gacaacacac caagtaatta tacgcgacga gttttcatgt 180 tgacgatccc tgagactata tcatacaaaa ttgacccaaa taataaactc gttccaatag 240 ttgaagtgtt gattaacaca ttgagacaag ttgtccg 277 <210> 285 <211> 276 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 285 gagtaaacga acatccaaca gtatgtacaa tcgtatgaga aaggacaaat ctgaagacga 60 tattagaggg agtccaacaa gtttgtgatg attcacgtaa ggaaatcgaa atgtaagatt 120 attcgtcaat ggaggtataa gacaacacac caagtaatta tacgcgacga gttttcatgt 180 tgacgatccc tgagactata tcatacaaaa ttgacccaaa taataaactc gttccaatag 240 ttgaagtgtt gattaacaca ttgagacaag ttgtcc 276 <210> 286 <211> 202 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 286 gatagtaaaa gtcgaaatct ttatattcgg ttcgatgtac tacgttattc gcgactgttc 60 tgtcatgata gttttttata tagtcctaat aaaaggagtt tttaaagtaa atgacatttc 120 agtagtaaaa gtttaagagc tttttgttct gccagacgtt ctgatagttg aggaaaacgt 180 gtggtcagct aataacttgt gg 202 <210> 287 <211> 211 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 287 gattagatag taaaagtcga aatctttata ttcggttcga tgtactacgt tattcgcgac 60 tgttctgtca tgatagtttt ttatatagtc ctaataaaag gagtttttaa agtaaatgac 120 atttcagtag taaaagttta agagcttttt gttctgccag acgttctgat agttgaggaa 180 aacgtgtggt cagctaataa cttgtggtat g 211 <210> 288 <211> 77 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 288 gtacgaatta cgaacacctt aacattaggg aaacttagat agttgtacaa agtaacgtat 60 aacaagtatc gcgtatg 77 <210> 289 <211> 167 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 289 atgacgagaa gcatttcaag aaagcgttgt agcttaaaaa gaacgttgcg gtgctaaagg 60 agaaagtcga aaaagcgaag gagttaaaac aaaagccggt tattaaatgc tggaagaagg 120 aagaacaaca actaaacaag gttgtcaaga gcattaagga agccgta 167 <210> 290 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 290 gataccgaat ttagaggcca taggttatgg aaaaagtcag tg 42 <210> 291 <211> 61 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 291 gtggagaagc aaatacttgg ataccgaatt tagaggccat aggttatgga aaaagtcagt 60 g 61 <210> 292 <211> 80 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 292 tggccaaaca acactcgcaa agaatttatg tgcgcttggc aggtggagaa gcaaatactt 60 ggataccgaa tttagaggcc 80 <210> 293 <211> 661 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 293 gttaccatgt aagaagcggt agacgccatg gcctagtagg aagaatgctt gttcacggag 60 ccgacgaagt tagacgagtg gcagaagaaa gagcattatc ttggtactcg gactgaatgc 120 gtactacggt cgtccaagtt gcaagaggta ttagagtcat tcgaatagct gtgggagtgg 180 tatttatagc ataataagtg taaaaaacat tcccaacagc atatgtgtag ccaagaagcg 240 ttgtgctcgc gactaaaagt caggtagcag caggcattaa cccagtgata gaacatcaaa 300 gtcaaataag tatggtttag cattactgcg tatcttgcga ttgcgtgctg gtggtcaaca 360 gtgcaaacct agtggcttcg cactcaaatg tagacaagca ttaaccaact acttctaacg 420 tatgttcagg aagaatggct attctcgtca ggtgaatgcg taatcgaatg agtagtatgg 480 tcgaacgtcc cgctatagtc tgacacgatt caggcaaagt tggagtgcaa agccatgatt 540 tggtcgatgc ggcaaatgtt gcttaggact attctatggt cttgcgtaat tgtgaagatt 600 tgccagttat ctaaggagac gtggtgattc gctctatagc tgtatctaat ctgccagatt 660 g 661 <210> 294 <211> 627 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 294 gttaccatgt aagaagcggt agacgccatg gcctagtagg aagaatgctt gttcacggag 60 ccgacgaagt tagacgagtg gcagaagaaa gagcattatc ttggtactcg gactgaatgc 120 gtactacggt cgtccaagtt gcaagaggta ttagagtcat tcgaatagct gtgggagtgg 180 tatttatagc ataataagtg taaaaaacat tcccaacagc atatgtgtag ccaagaagcg 240 ttgtgctcgc gactaaaagt caggtagcag caggcattaa cccagtgata gaacatcaaa 300 gtcaaataag tatggtttag cattactgcg tatcttgcga ttgcgtgctg gtggtcaaca 360 gtgcaaacct agtggcttcg cactcaaatg tagacaagca ttaaccaact acttctaacg 420 tatgttcagg aagaatggct attctcgtca ggtgaatgcg taatcgaatg agtagtatgg 480 tcgaacgtcc cgctatagtc tgacacgatt caggcaaagt tggagtgcaa agccatgatt 540 tggtcgatgc ggcaaatgtt gcttaggact attctatggt cttgcgtaat tgtgaagatt 600 tgccagttat ctaaggagac gtggtga 627 <210> 295 <211> 112 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 295 gattacggca cttgtagagc acgtatcaga cccagctttg aaagaaggct gagttcggtt 60 cgggtttcac gtaggcctag aacgaggtat agaagtggtt aacaaagatt tg 112 <210> 296 <211> 91 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 296 gatcactgca aaaaggacta ctcaataatg gtttctaaag tcattatgct ggtgctccct 60 atggcggtgg tcagtactgt aactaaagat g 91 <210> 297 <211> 128 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 297 gattccacat ccgttcttgc aattaaagaa acgatgtaga aaacgctttg gttggcgaca 60 acttgggcgg caaaataatt atggtaggta attgaagtag cgttaaaacg caaaaagaag 120 gaagcacg 128 <210> 298 <211> 129 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 298 gaaagaatag ctgaactcaa agaagccgac aaagataaac aaagaagaaa cggttaatac 60 gattgaggag aaatattcaa tccaacggca actattaacg tttcaactag aatctacgtt 120 tcatacgcg 129 <210> 299 <211> 222 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 299 agcgctagtc catgaaaatg tcctagcttt gaacgtttat caaatgcaag ttgtgggagt 60 tagtcatgtg agagttgtgc aagaaagtgt taacgttcta gccgtgctca gtaacaatta 120 ccattcggcg gtaacaaacg taagttgtca ctgcactcat gagcatgaag ttagaccagt 180 agttgggtca gaaattagac gtggtttagt ctggttaccg tg 222 <210> 300 <211> 269 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 300 gaattcaatg cagaaactcc tatgcacgtc gaactagtct cttgctcagc gctagtccat 60 gaaaatgtcc tagctttgaa cgtttatcaa atgcaagttg tgggagttag tcatgtgaga 120 gttgtgcaag aaagtgttaa cgttctagcc gtgctcagta acaattacca ttcggcggta 180 acaaacgtaa gttgtcactg cactcatgag catgaagtta gaccagtagt tgggtcagaa 240 attagacgtg gtttagtctg gttaccgtg 269 <210> 301 <211> 182 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 301 gatggttagc gtaaacatgg tggaacgtac tttgactgcg ctgcaagaaa tggttgggtc 60 gatcgtaatg ctagtcgtta catcggaaca agccaaaaca aatcattcgc tggatttaga 120 cctactgcac gacgacgtcg acacaagaca ttcttgaaag gtaattgacg tggacgtttc 180 ag 182 <210> 302 <211> 166 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 302 gatggttagc gtaaacatgg tggaacgtac tttgactgcg ctgcaagaaa tggttgggtc 60 gatcgtaatg ctagtcgtta catcggaaca agccaaaaca aatcattcgc tggatttaga 120 cctactgcac gacgacgtcg acacaagaca ttcttgaaag gtaatt 166 <210> 303 <211> 160 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 303 gatggttagc gtaaacatgg tggaacgtac tttgactgcg ctgcaagaaa tggttgggtc 60 gatcgtaatg ctagtcgtta catcggaaca agccaaaaca aatcattcgc tggatttaga 120 cctactgcac gacgacgtcg acacaagaca ttcttgaaag 160 <210> 304 <211> 128 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 304 cttccccatt tgaagtcatg tagtggttgt ctactttttc attgaagacg tccacctcaa 60 cgctatgaac gttagttgag acaatgttaa agcaaacgac aacgtcattg tgatctacat 120 acacagtg 128 <210> 305 <211> 144 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 305 gctacgtggt ggatgaacat ggatgcgaac gacatttcga aatatacgca atgatatcca 60 accctaccga aacagcaata attgtagaag gtagcatatc atgccgagtc atcgacatgc 120 ctttatcgac acacgacgtg attg 144 <210> 306 <211> 120 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 306 gtgtatttat ccgctacgtg gtggatgaac atggatgcga acgacatttc gaaatatacg 60 caatgatatc caaccctacc gaaacagcaa taattgtaga aggtagcata tcatgccgag 120 <210> 307 <211> 124 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 307 gttggtgtat ttatccgcta cgtggtggat gaacatggat gcgaacgaca tttcgaaata 60 tacgcaatga tatccaaccc taccgaaaca gcaataattg tagaaggtag catatcatgc 120 cgag 124 <210> 308 <211> 424 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 308 ctgtcaagac ccttctataa tagtcgaacc agtacggtaa gtgggcaaca tgcccatata 60 agtcgaagca tttcaaatgg ttcatccagt agacgacgat gtcctagaca gtcatgtctg 120 tcacatctat cacgcctcct acgttgtgcc ataacgaaga ataatagtga taagagacat 180 cgaagctgtc gtagtcgttc gctaaattgc ttacaattta gtgctctaag tgaacaatgt 240 ttcatcttga gaagcaaacg aagtagaagt tgacgacgca ttcgaagacg tggacaatgt 300 gacacgagta tcgaaacatg atccaacgaa catcgaagaa gttactactg aactacgggt 360 cagcttcccc atttgaagtc atgtagtggt tgtctacttt ttcattgaag acgtccacct 420 caac 424 <210> 309 <211> 513 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 309 gagtggatgg aagtgtgttc gactgtcaag acccttctat aatagtcgaa ccagtacggt 60 aagtgggcaa catgcccata taagtcgaag catttcaaat ggttcatcca gtagacgacg 120 atgtcctaga cagtcatgtc tgtcacatct atcacgcctc ctacgttgtg ccataacgaa 180 gaataatagt gataagagac atcgaagctg tcgtagtcgt tcgctaaatt gcttacaatt 240 tagtgctcta agtgaacaat gtttcatctt gagaagcaaa cgaagtagaa gttgacgacg 300 cattcgaaga cgtggacaat gtgacacgag tatcgaaaca tgatccaacg aacatcgaag 360 aagttactac tgaactacgg gtcagcttcc ccatttgaag tcatgtagtg gttgtctact 420 ttttcattga agacgtccac ctcaacgcta tgaacgttag ttgagacaat gttaaagcaa 480 acgacaacgt cattgtgatc tacatacaca gtg 513 <210> 310 <211> 141 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 310 gagtggatgg aagtgtgttc gactgtcaag acccttctat aatagtcgaa ccagtacggt 60 aagtgggcaa catgcccata taagtcgaag catttcaaat ggttcatcca gtagacgacg 120 atgtcctaga cagtcatgtc t 141 <210> 311 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 311 gtaaaacttg tcacccccat ttacattgcg acagacacag ttatg 45 <210> 312 <211> 113 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 312 gagtttacta cgttgtagcg agtttagttg aaatagtttg tgatgtgtaa actaaaacat 60 accaaatggt aaaacttgtc acccccattt acattgcgac agacacagtt atg 113 <210> 313 <211> 175 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 313 caccgaattt catcaacaac attaataatt ccataggaac tacattctta aatccttgga 60 actccaaact cctaccttat cacggttcta tccgtcgtct tagggtcggt ctcctcgtag 120 catggcctcg ttctcctcgc cgtaattgcg gtcgaaagta cttcagttat ttatg 175 <210> 314 <211> 290 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 314 agactacaaa atctcaatcc tccattcccc cgtcaggtct atctaaaatt caatcgctgt 60 gtttgcgggg taaataatag gacaagtccg tataatctac ccaatcgcat tcattccagt 120 cagagggtac caccgaattt catcaacaac attaataatt ccataggaac tacattctta 180 aatccttgga actccaaact cctaccttat cacggttcta tccgtcgtct tagggtcggt 240 ctcctcgtag catggcctcg ttctcctcgc cgtaattgcg gtcgaaagta 290 <210> 315 <211> 360 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 315 gattcgtcga acgtccgggt agtatagtta tctgcatcgt ggtcaccagt atcacagact 60 acaaaatctc aatcctccat tcccccgtca ggtctatcta aaattcaatc gctgtgtttg 120 cggggtaaat aataggacaa gtccgtataa tctacccaat cgcattcatt ccagtcagag 180 ggtaccaccg aatttcatca acaacattaa taattccata ggaactacat tcttaaatcc 240 ttggaactcc aaactcctac cttatcacgg ttctatccgt cgtcttaggg tcggtctcct 300 cgtagcatgg cctcgttctc ctcgccgtaa ttgcggtcga aagtacttca gttatttatg 360 <210> 316 <211> 136 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 316 gttcgactag acctctcgtg gtagggcctg gaagtcaaaa gacttctcta tgggtactag 60 gtaggtagtt agagtacggt ccctggggtg tctcagagtc gaacgttcgg gggaagttcc 120 taaacatgtt aggatg 136 <210> 317 <211> 476 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 317 gatagtgggg gacctgagtc atactagggt aacttcttat atggtgacat cactcctacc 60 gtagaggggg gacggtagtc tgtgggaaat ctaccctagt gaagaaaggt aagagaatgt 120 ctgaacatta cctgtagacg aaggtactct cagaacaact actctcacat acggaaaggt 180 ttgggtcctt ggtcaaagga ctttactgac tctgcagacg atgaatatct gaaccaacac 240 tactatcaaa gatgtcgtag gtttgtgaga agggagaaga agtggttgag aagttggtgt 300 tccacaagtt aaagtagaca tacctttagt acctgtaaag gttcgactag acctctcgtg 360 gtagggcctg gaagtcaaaa gacttctcta tgggtactag gtaggtagtt agagtacggt 420 ccctggggtg tctcagagtc gaacgttcgg gggaagttcc taaacatgtt aggatg 476 <210> 318 <211> 255 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 318 gatagtgggg gacctgagtc atactagggt aacttcttat atggtgacat cactcctacc 60 gtagaggggg gacggtagtc tgtgggaaat ctaccctagt gaagaaaggt aagagaatgt 120 ctgaacatta cctgtagacg aaggtactct cagaacaact actctcacat acggaaaggt 180 ttgggtcctt ggtcaaagga ctttactgac tctgcagacg atgaatatct gaaccaacac 240 tactatcaaa gatgt 255 <210> 319 <211> 256 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 319 gtgtttaggt gtgtcggagg gcagaaacca acgaaagaaa ggaaacgatc aacacttata 60 tagtgtcctg tcgggcccaa atcctaagag accttaggtt taccacatta gtactaaaca 120 gcctgttgta taccgtgagc ccccacttcg atctgtaaat ggtaatccct tgtaaaacct 180 catctccaaa aacagtatac gcaggtacag ctgatgtgat cctctctact agtcttctct 240 gaggaaccat gagatg 256 <210> 320 <211> 262 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 320 gagtaggtgt ttaggtgtgt cggagggcag aaaccaacga aagaaaggaa acgatcaaca 60 cttatatagt gtcctgtcgg gcccaaatcc taagagacct taggtttacc acattagtac 120 taaacagcct gttgtatacc gtgagccccc acttcgatct gtaaatggta atcccttgta 180 aaacctcatc tccaaaaaca gtatacgcag gtacagctga tgtgatcctc tctactagtc 240 ttctctgagg aaccatgaga tg 262 <210> 321 <211> 203 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 321 acgtataggt gtgctctcag ctgccgttcc ggcgctgctt cacccctttc cgcttatcgc 60 ggatggtaaa gtcaaagttt actcagcggt cgctgttgac ctcaggttct ctatcaaaaa 120 tttgctggct aaaacggtcg gagcttctgc tccactcttt ccagcaatcc ttctgccagt 180 cgccttcaaa cttgcaatct gtg 203 <210> 322 <211> 240 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 322 gtgaggcatc tataccgcca cgccctatgt ctttctcacg tataggtgtg ctctcagctg 60 ccgttccggc gctgcttcac ccctttccgc ttatcgcgga tggtaaagtc aaagtttact 120 cagcggtcgc tgttgacctc aggttctcta tcaaaaattt gctggctaaa acggtcggag 180 cttctgctcc actctttcca gcaatccttc tgccagtcgc cttcaaactt gcaatctgtg 240 <210> 323 <211> 210 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 323 gtgaggcatc tataccgcca cgccctatgt ctttctcacg tataggtgtg ctctcagctg 60 ccgttccggc gctgcttcac ccctttccgc ttatcgcgga tggtaaagtc aaagtttact 120 cagcggtcgc tgttgacctc aggttctcta tcaaaaattt gctggctaaa acggtcggag 180 cttctgctcc actctttcca gcaatccttc 210 <210> 324 <211> 84 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 324 gtacaagagt tggttactct cagtcccatt cccaacacat cactgagaag tatagagcct 60 agtggtcatt agaaggtcaa catg 84 <210> 325 <211> 67 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 325 aagccttgaa acggtctgtt cctcgtgtca agttctttgt ccgcttatac cgccatctct 60 cgttgtg 67 <210> 326 <211> 116 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 326 gttttacaaa ggagaacttc caacatcagt gtatgggatg tttcaaacac ccccagagac 60 ggagacaata gtgtggtcac gtacatttag gaagataaaa ataacaacaa cagaag 116 <210> 327 <211> 342 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 327 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acagctatcc 240 tgaggtgcca ggttagaata ggccctacca catctaagcc ttgaaacggt ctgttcctcg 300 tgtcaagttc tttgtccgct tataccgcca tctctcgttg tg 342 <210> 328 <211> 234 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 328 gcttgtccgc gagtgtcaac cgggttttac aaaggagaac ttccaacatc agtgtatggg 60 atgtttcaaa cacccccaga gacggagaca atagtgtggt cacgtacatt taggaagata 120 aaaataacaa caacagaagg tgtgaggcgc aaggtacatg ctcttacccc actctcagca 180 cgtagggagc ggagtgtgga agaccaacaa gcccgtcgcc tctaccaaat acag 234 <210> 329 <211> 484 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 329 ttatgataac aagtccaaac gtggacgtcg tcgctgtcac ctctcggaac actagctgag 60 gtggcttaaa aacattccgt aactgagtgg tcaccggggg tgaaacatgc tgagggttct 120 accttaacgg gaaggtgaaa ctctccaatg tctttgaaag gactagttga gataacccaa 180 accgaatccg gaggaagagg agaacttgga aaacgttgta cgacgtcatt aataacgatt 240 ccgttcttcc cgttcgtgtc tataaggata ttctttcatt gcgaaggtaa gcccttttgg 300 gtctagatac ggactatact caaaaaccca cagaccttcc acttggagtt gttttaggag 360 acggagtagt agagaaaact tgtggcacct tcccagtcgg ttccctacag actctcctta 420 gtagctccta agatcgtcga gttacacgac gacattgttc cgactgacgt agaggcatta 480 cttt 484 <210> 330 <211> 478 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 330 ttatgataac aagtccaaac gtggacgtcg tcgctgtcac ctctcggaac actagctgag 60 gtggcttaaa aacattccgt aactgagtgg tcaccggggg tgaaacatgc tgagggttct 120 accttaacgg gaaggtgaaa ctctccaatg tctttgaaag gactagttga gataacccaa 180 accgaatccg gaggaagagg agaacttgga aaacgttgta cgacgtcatt aataacgatt 240 ccgttcttcc cgttcgtgtc tataaggata ttctttcatt gcgaaggtaa gcccttttgg 300 gtctagatac ggactatact caaaaaccca cagaccttcc acttggagtt gttttaggag 360 acggagtagt agagaaaact tgtggcacct tcccagtcgg ttccctacag actctcctta 420 gtagctccta agatcgtcga gttacacgac gacattgttc cgactgacgt agaggcat 478 <210> 331 <211> 209 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 331 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 120 agataaaaat aacaacaaca gaaggtgtga ggcgcaaggt acatgctctt accccactct 180 cagcacgtag ggagcggagt gtggaagac 209 <210> 332 <211> 146 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 332 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 120 agataaaaat aacaacaaca gaaggt 146 <210> 333 <211> 144 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 333 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc ccagagacgg agacaatagt gtggtcacgt acatttagga 120 agataaaaat aacaacaaca gaag 144 <210> 334 <211> 80 <212> DNA <213> Artificial Sequence <220> <223> exon <400> 334 gtgaggcttg tccgcgagtg tcaaccgggt tttacaaagg agaacttcca acatcagtgt 60 atgggatgtt tcaaacaccc 80 <210> 335 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> XhoI site - T7 promoter <400> 335 ctcgagctaa tacgactcac tatag 25 <210> 336 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> polyA-tail - NsiI site <400> 336 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tgcat 35 <210> 337 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer <400> 337 gctaatacga ctcactatag 20 <210> 338 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Primer <220> <221> variation <222> 26 <223> v = any of a,c,g <400> 338 tttttttttt tttttttttt tttttv 26 <210> 339 <211> 12643 <212> DNA <213> Artificial Sequence <220> <223> SIRV1 <400> 339 agagttcctt cctccatacg cagttatgca agtcattgct gtatcccata aacctgtcaa 60 agtcactgta tccggttatg taaggtctag aggaatgcgt agtcatttat ggaaatatct 120 cagctaagtg tagttgcggt aaaatgtgtg tagcggctgc tgttttcccg actttcaccg 180 tgagatgatt aaacaagtac actcttgtta tttgttataa gctcatgtgc agctacccta 240 tcacgagagc ggtccggcgg gtgaagcatt aacaggaaac tcatcacgtc ggcgtaatta 300 aatcgtacac cggtcgtgga atcgcacttc gctagcagtt taacatgcgt gcggtccaac 360 cctacataca cgatacctga aaatcgcgat gggcttagta acgggtagct aaaacggtat 420 tccgaagcgg cgcatcaatt cgctaaggtg tggttgttct cattacacgg cttagataca 480 attatagtgg caaggaacac aattagttat taccaacggc gacgaatagt agtttacttc 540 ctccctgcgg gcccctcctg aagtgccacc tatacggctt gttgaagccg attagtacaa 600 tagatttatt caaccccaaa ggtctacact cccggcttac tcttagctga tatgtcgcgt 660 cgagtcccgg gtttgagtgc gttagcgccg ggttgtctgg cccagttcga cttttgggta 720 cctcatcggg ttacactggc tgacacttat tcttcagata aggttccctt attgggacta 780 gtaactgagg tttgaaacta tccctacttc taatggtctt aactctttcg catgaatgac 840 ggtacgagct taccttacga ggttatcact tatccaagtg cagggtgctc ttccagctct 900 tgaagaggac gtagagagtt ggatgtatta gatgggcgta cgcattatgt gcccacttgt 960 atgattgtgc gttttcaagg aaggaagtat gcatcgattc aaagtaatgc ctctacgtca 1020 gtcggaacaa tgtcgtcgtg taactcgacg atcttaggag ctactaagga gagtctgtag 1080 ggaaccgact gggaaggtgc cacaagtttt ctctactact ccgtctccta aaacaactcc 1140 aagtggaagg tctgtgggtt tttgagtata gtccgtatct agacccaaaa gggcttacct 1200 tcgcaatgaa agaatatcct tatagacacg aacgggaaga acggaatcgt tattaatgac 1260 gtcgtacaac gttttccaag ttctcctctt cctccggatt cggtttgggt tatctcaact 1320 agtcctttca aagacattgg agagtttcac cttcccgtta aggtagaacc ctcagcatgt 1380 ttcacccccg gtgaccactc agttacggaa tgtttttaag ccacctcagc tagtgttccg 1440 agaggtgaca gcgacgacgt ccacgtttgg acttgttatc ataactgatt atgagtaacc 1500 gcaagacagt gtaaacactg taatccatac ttgattgaga tcccttgaaa atgctggtac 1560 cattttctaa actcgatggt ttgttgacaa ttatttgtac agcgacgcat ctagcggttc 1620 ttggtcgacc ctactcacgc aataaatgca gcacgttcga cccagactca tccatggtag 1680 cttcaaatac ggattcaacc tggcgtttat atccagaaaa cggccctagg ggtgatagac 1740 acttaacact ttgggcaacc attggacatg tttcacgtat cgatatagta accattattc 1800 tgcatttgcc tcctagcgag tgaggaagaa gcctttctat tggcttctta atcctcccat 1860 accacgcgcg tacctttccc ggcccttcat tgagaccgga agtgccttgt tattcaatgt 1920 taaacccttg tcagcctatc gcggtgatgc acgaaaaaac caaatggagt atagagcatc 1980 aactactccc aattttaagt gccctcttct aggttgcatt catatatcaa tgtagacttc 2040 gcaagctagt gaatgaggga aatgaattta tccggaatga caaaggggac cgagcattca 2100 agatgcgaac cgttagatgg gcgttccagt ctgcacagta agcctttgtt ttttaaccat 2160 tgaaggaatc gacttcacga cccttgcgag ttcagtcatc agaagaagag accttcagcg 2220 ctccccccct gaaatagtac agcttcaaac aataacctga gtaagttggt atccgaggta 2280 ctatgagttt ctcgaccgtt cgacgccccc atcctatgtt taaagagctg tcatccctag 2340 cctcgagctt acacaggaac cagctgagtc taggctgggc ccacttgggt tgcttgcata 2400 tacaaaatga tgtacaccgc aggacctgtc ccttagggaa ttgtggttga ccaaaagtcc 2460 gctacccatc ggcgtcgtgt cacttttgtg ccgataggta tgatcaatct cttggaggct 2520 attacgatgt tggtaaaaga ttagaactgc tagacagctg ttgataatca agtcgctccg 2580 tagattgccg agaatcagtt gagatgagta cgttatttag ggcgcacgag ctgcgattga 2640 gttggtacca tcgtagttta cgcctatgtt agtccccaat tatacgccct ttgcccgaat 2700 ccccactatc tgtgaaatgt ttaacgcgtt cgctacctct acaaaataca tagctacgtc 2760 atacgtaaga atatcttaac ggaggatcgc acactctctc ctcggacaga taagcgggga 2820 agtaggaggg tatggtgcac gcatggaaag ggccggtaag taattctggc cttcatggaa 2880 cgatatgtta taatttggaa attgtcggat agcgcgacta tgtgcttaat tggtttactt 2940 caaatctcgt agttggtgac agtaaaaatt cgccggagaa gttccagcgt aagtatatgg 3000 ttacaactga acgattccag gatttactcc cttaagctaa ttagacctat ctgtttcccc 3060 tggctcctaa gttttgcggt cggcgattta cccgcaagga gacgcatgtc attcggaaat 3120 aaaaaattgg aaacttcttt ggctgttgtg ctgggaacgc tcatgtcaat acttttcttc 3180 tctagaagtt gaaagggggg gatattatca tgtcgaagtt cgttataaga gtcattctac 3240 catgcacacc atgattctga aagtgctggc aagctgcggg aacgagatac atatttctca 3300 tcactagggc tcagacctca agtgagtcgt tggtcaactc aggtccgagt cggaagaacc 3360 caacgaacat gtaactctca ctacgcgtgt tgtcctggtc agagcactcc ttaacactaa 3420 ctggttttca ggcgatacgt agctgcggca gagtcaagtc atgggtattg atagtagtta 3480 gagaaccacc gataatgtta caaccgtttt ctaattttga cgttgtgtgg gcaactatta 3540 gctgagcgag ctaactaaca gctcttacaa atctctactc atacaataag ttccgtgagc 3600 tcaacactaa ctcaacctgg gtaacatcag atgccgatat attcaggggt tattatgcga 3660 gatatgcgct tagggatagt agacacttca caagttgcgc atgcgatcga gacattttat 3720 gaatcgatgc aatgtgcatt cttatagaat tgcctgttag cgtaacatag gtgaacgtgc 3780 ctaattaccc tctcacccgc gcttattgca tccgtaccat ttccggccat tcattaagat 3840 cggaagtacc ttgcgataca aaattaaact tcaaatgccc tatcacgcgg atttagcagt 3900 tattttaatc aactttagtg gaccaatcat agacattttt aggcggtatt ttcggggtgg 3960 catttaaata ctaatgatga catattaggg tcttaaatga gagaattcga ttaatttggt 4020 tagacaaggg gatccgaaga tattttaccg cagccactaa aagggcgtac ctctgcctac 4080 aataagcctt tattcttaaa tgtctgaaga aaccgcgaag acggctgtcg tcaatacaaa 4140 tatgataaga gcagtactac atctttgctc cgcaataacg gtacagcttc aggtaatatt 4200 cacagtaaaa tggtacgtcc ggtactaaga ctttcatgac tgttcgacgt ttatgcttta 4260 ggtatgaaga gtagtggccc ggtgtctgga gatcatccta catccgattg actccaagca 4320 cagccttctc ggcttgctag tacattgaga gtgatacgct caactggacg agactcgaga 4380 ggaaatacga ctgaccgtaa gaccgctttg ttccgacacc gtcttagcac agcactcatg 4440 attatcatca ctcttttagt gcctattaca atgctggcaa gagaataaat ctccatcaca 4500 ctccttgaaa atgcacttgg tcgtttgatt gttgagaaag tttaatgatg agtatgttat 4560 tcaaggcact ggtgtactga tagagttggg ctagatgtta actacgagtg tataaccctt 4620 caatgactcg ctctatacgc aaattcctat catctgtgaa gtgttcaacg cctatggcaa 4680 ctctgtagaa tattcatctg cgttacacct tagaatatct tagcgggcta tcgcaacaca 4740 tccacttgtg ccgattaatg ggagagtggg cgcgataatc gaaggtatgg tggacgccgg 4800 taggtaattc taggcgacaa ggaatgctat tttcttaatt attctttagg gcattgtacg 4860 cctaaatcat cagcaaaatt tgttgaaatc acctcgcttg attttgtaaa gatctgtctt 4920 aaaaccgtca ccgttaatta gagattacta cactataatc ccagaattta cattcttaag 4980 ctatttaaac taacctgttc ccctaggctt ctataaaacc gcgtcagtga ttatccccct 5040 cggagacgga tgtttttcgc gaataacaat tgacagattc ctatgtcgct tccgccgtca 5100 gcaattttgt tttcactata ctcgttgtga cgtagattcg tcgcgttatt gcgttgtcga 5160 actccataaa aagcgtctct ttgccgtgca ggtcaagatt gtgaaagtat agacgagcag 5220 caaatacgaa aaccgcactt ttgaccacgc ggccacagac ctctggtagg aagaacgctt 5280 actgaggttc gtgtcggtag tgccaaacat ccatgtgacc ctgacagtgc gaattgacct 5340 gccttgagct cttccatatg ctgactagca ttctggcgaa acaaggctgt ggtagtattg 5400 tgccgtgagt actaatagta gtgaaaaaat cagggataat gtctcgacgg ctcacttact 5460 tagaggtagc gtgagacacc tttacgtgaa ctagcaaaac gacatctctt ttaaattatt 5520 tgtcttacag ttacttccgt gatgacatga ttatcccggc ccgatgtaca attgatgttc 5580 tcatattgga aaactactgg gccagatatg tattaaagca tagcacgtac atcgcaagtt 5640 ccggatatcg ttaagactac ttataagtaa tcccattgcg gaatcttata atatgacccg 5700 atagcgttgt gttggtggag acggcagatt acgctctcac cggcgctatt agcttccata 5760 ccagctggca acatccatta agatccgcta ctcctcagga aaaaagtatt tataagaagt 5820 cctgtaacat gtgcatttga tagtcctaat tgacaacatt actcgggcga actaaaaccc 5880 ttgagcacag aattttagca gaggctaata atgtgatatg atatgaaatt agggtctata 5940 tagttataat ttggtgagtt aaattggatt aggagtttgg aagatgattt ggcacagtta 6000 gtcacagacg aaggacttgc cttcaaaaag cggttactgt tgtctgtctt gggtttcagc 6060 gaaagcagga gtcgttgaag caaaagtaat atgtccgaca ttgcatctag gcaaggcaac 6120 ttcgcaacag gtcaacctgt tctttgtaga gaaactagag gtccagttca aacactctca 6180 tttgtgcagc tcgtttatgc atacggcgtg taaactggtg cgtcggtgta tggagacctt 6240 ccttcttgcg tatgattgca tggactgcga tcacgttttg tgggttcact gggactgtca 6300 cgcttaatcg ctcggaattc gagaaagcaa acgacaccat cctaacatgt ttaggaactt 6360 cccccgaacg ttcgactctg agacacccca gggaccgtac tctaactacc tacctagtac 6420 ccatagagaa gtcttttgac ttccaggccc taccacgaga ggtctagtcg aacctttaca 6480 ggtactaaag gtatgtctac tttaacttgt ggaacaccaa cttctcaacc acttcttctc 6540 ccttctcaca aacctacgac atctttgata gtagtgttgg ttcagatatt catcgtctgc 6600 agagtcagta aagtcctttg accaaggacc caaacctttc cgtatgtgag agtagttgtt 6660 ctgagagtac cttcgtctac aggtaatgtt cagacattct cttacctttc ttcactaggg 6720 tagatttccc acagactacc gtcccccctc tacggtagga gtgatgtcac catataagaa 6780 gttaccctag tatgactcag gtcccccact atcctagctc tccttaatgc tgactggcat 6840 tctggcgaaa catagctgcg gcagaatcga gtcgtgagta ctaatagtag taagaaaatc 6900 acggataatg ttacgaccgt tctcttattt tgaggtagtg tgaggaactt ttacctgaac 6960 cagcaaacta acaactcttt caaatcacta ctcatacaat aagttccgtg agcacatgac 7020 tatctcaacc cggataacaa ttgatgctca catattggga agttactgag cgagatatgc 7080 gtttaaggat agtagacact tcacaagttg cggataccgt tgagacatct tataaataga 7140 cgcaatgtgg aatcttatag aatcgcccgt tagcgttgtg taggtgaaca cggctaatta 7200 ccctctcacc cgcgctatta gcttccatac cacttgcggc catccattaa gatccgctgt 7260 tccttacgat aaaaaaatta ataagaaatc ccctaacatg cggatttagt agtcgtttta 7320 aacaacttta gtggagcaaa ctaaaacatt tctagacaga attttggcag tggcaattaa 7380 tcactaatga tgtgatatta gggtcttaaa tgtaagaatt cgataaattt gattagacaa 7440 ggggatccga agatattttg gcgcagtcac taataggggg tgcctctgcc tacaaaaagc 7500 gcttattgtt aagtgtctaa ggatacagcg aaggcggcag tcgttaaaac accatctcat 7560 ggttcctcag agaagactag tagagaggat cacatcagct gtacctgcgt atactgtttt 7620 tggagatgag gttttacaag ggattaccat ttacagatcg aagtgggggc tcacggtata 7680 caacaggctg tttagtacta atgtggtaaa cctaaggtct cttaggattt gggcccgaca 7740 ggacactata taagtgttga tcgtttcctt tctttcgttg gtttctgccc tccgacacac 7800 ctaaacacct actcctaaag catgtacatc gcaagttccc gatatcgttt aggctaccat 7860 ttactaaccc cattgcggaa tcttattata tgatcggata gccttgtgtt cgtggagacg 7920 gcacattagg ctcgcaccgg tgctattacc ttccatacca gctggcaaca acgattatca 7980 tccgctactg ggcaggaaaa aagtatttaa aagaagtctt gtaacaaatg tattacatgg 8040 tcctaattgg ctacatcact cgggcgaacc aaaacccttg agcacaaaaa ttaagcagag 8100 gctaatcgcg tgatatgatt taaaatttcg gtctgtatgg ctataatttg gtgagttaaa 8160 ctggattagc agtttgctag acgatttggc acagttagtc acagacgaag gactcactat 8220 gaaaaggcgg tttcagttgc caatcttgga tttcagcgaa agcaggaacc gatgtggcta 8280 aagaaatacg tccgtcattg catctaggta aggcatcttg ccgaaaagtg aacctgtact 8340 ttgtaaagga actagaggtc ctctttatgc tctgtggttt gtgcagctct tttatgcata 8400 cgacgagtaa acaggtgcgt gggtgtatgg ggaccttcct ttttatgtat gatcggatgg 8460 actgcgatca cattttatgg gttccctggg actgtcacgc taaatcgctc ggaaattgag 8520 aaagcaatcg actacttgca atgacgcact gaccgaactc gagcataacg ggtcacacac 8580 cataatcttc gcctctttag tcccagtcat tcagcaccgc ggtagacgtg taacaaccgg 8640 ccactgccca tctacatatg atcgtattgc tttagaatta acttaataaa taggatgcgc 8700 ggggaggatc tactgtactt atgcgaccac gctacgtact aacttgaagg ctacgtccta 8760 ttcttggcct tgtgcataca taacatgaat acgtacatgt aggattcaga agttgtagca 8820 aatcccacgc tgaacgtttg gggaaaggcc tcagcattat atcctagcct gtcggaacac 8880 aagcaccttt cccatgtaaa cccagcgtca tcaccacaat gaatagtccg accgatcgtt 8940 attgctatta gtacatgatg actcgtcctt ctaacacaaa ttttctactg aatattgatt 9000 acaaaatgta ccaggattag ccaacgtggc gagcccccct ggttttgggt actggtgatt 9060 ttatttcgac tcagatcagc gcactaaact gaattctaaa gctagacatg cagatattaa 9120 agctctctat ttgacctagt cgataaacaa tcagctagac cgtgtcttac tgtgtctgct 9180 tcttgagtga taccttaccg ccgaagtcga cggttactac ctagggtcgc cttgatcctt 9240 cgctacaccg gactccttat ggagagagta acgtaaatct attttgtaga gcgacctttc 9300 ccttggtcat gaaacatttc gttgatctct aagagaaata cgagacacca agcaggccaa 9360 gaattttcgt ttgctcctca ttagtccacg cacccgtttt cgcttgggag taaagattca 9420 tgttagtcta cctgatgcta acgtaaaata ccctagggac gccgacagtg ccattatgtg 9480 agcctcttac tgttaggtta ggtgttgatc gatactgggt gactggcttt acctcgtatt 9540 gcccagagtg cggtatttaa aacggtcaaa ttaggcccag aaagttgtaa cgccatccgc 9600 acattgttgg ctggtgaccg gtaggtgtac aataatataa ggtattcaaa attgaattat 9660 atatcctttg cgcccgtcct agatgacacg aacacagtgg aacgatgcat gattctgcat 9720 tcgatccagg ttatgagcgg gaactcgtat ttaaagtttt tgtccctgtg aaacctgagc 9780 cttcaacatc gtttagggtg ggacttgcga accccttgcc aaagtcgtaa tataggatcg 9840 gacaatcctg tcttcgtggt aagggtgcat ttgggacgct gaagtagtgt aacttatgag 9900 ggaggctaat tatagcaaaa actatatacc agtgagctgg aagattgtgt ttaaaagatg 9960 actaataaat attgttgtac atgctcctaa tcggttccgc ggctcgcggg caccagaacc 10020 catgacctct aaaatagagc tgagtttaga cgcgcgattt cacttaagat ttcaacctgt 10080 acgtctattg tatccaggga tatactgcat cccctatcag tctatcgatc tcgcatacaa 10140 tgactcagac gaagaactca ctatggaatc gggggatcgg gcgccaacga tcgttccgat 10200 cagaacaaga aagctatgtg gcctgacgaa tacttctctc attgtgttta gataaaagat 10260 cccgctggaa taggatccag accatgttga ccttctaatg accactaggc tctatacttc 10320 tcagtgatgt gttgggaatg ggactgagag taaccaactc ttgtacctat attaacactc 10380 aagagttgct tcctccatac gcagttatgc aagtgattgc tgtaatccat aaacctgtca 10440 aaaccacaac gagagatggc ggtataagcg gacaaagaac ttgacacgag gaacagaccg 10500 tttcaaggct tagatgtggt agggcctatt ctaacctggc acctcaggat agctgtattt 10560 ggtagaggcg acgggcttgt tggtcttcca cactccgctc cctacgtgct gagagtgggg 10620 taagagcatg taccttgcgc ctcacacctt ctgttgttgt tatttttatc ttcctaaatg 10680 tacgtgacca cactattgtc tccgtctctg ggggtgtttg aaacatccca tacactgatg 10740 ttggaagttc tcctttgtaa aacccggttg acactcgcgg acaagcctca cgtgtcatgt 10800 ccaactaccc tatcacgaga gcggtccggc gggtgaggca ttagcaggaa tgtcatcacg 10860 tcggcgtaat taaatcgtgc agcataaata actgaagtac tttcgaccgc aattacggcg 10920 aggagaacga ggccatgcta cgaggagacc gaccctaaga cgacggatag aaccgtgata 10980 aggtaggagt ttggagttcc aaggatttaa gaatgtagtt cctatggaat tattaatgtt 11040 gttgatgaaa ttcggtggta ccctctgact ggaatgaatg cgattgggta gattatacgg 11100 acttgtccta ttatttaccc cgcaaacaca gcgattgaat tttagataga cctgacgggg 11160 gaatggagga ttgagatttt gtagtctgtg atactggtga ccacgatgca gataactata 11220 ctacccggac gttcgacgaa tcctgaacgc aactagttat tacgaacggc cacgaatagt 11280 agaaaacttc ctccctgcgg gcccctcctg aaatgccacc tatacgggtt gttgaagccg 11340 gttactacga cagatttatt caaccccaaa ggtctacact cccggcttac tcttagccga 11400 tagcacagat tgcaagtttg aaggcgactg gcagaaggat tgctggaaag agtggagcag 11460 aagctccgac cgttttagcc agcaaatttt tgatagagaa cctgaggtca acagcgaccg 11520 ctgagtaaac tttgacttta ccatccgcga taagcggaaa ggggtgaagc agcgccggaa 11580 cggcagctga gagcacacct atacgtgaga aagacatagg gcgtggcggt atagatgcct 11640 cacgcaactt cggctaatca tgttatctaa ataagttggg gtttgcagtt gtgagggcga 11700 gatcagattc gactatacag cgcagtccgg agccctaact cacgtaatcg cgaccctaca 11760 gagcgggtca agctgatgac ccatcgagta gctcaatgtg agcgagtgtg agtaggaagt 11820 ctgtcgcaag gcaataatcc taatcattgg cagcaagctt tgataaggat gaaggttagc 11880 acaattcaga aggcgtactt actgccatac tcgaacggaa tgctttatta aagaaaaggt 11940 tgacgtccca cgggaagatc gagaacttct cctgcatctc acaacctaca taatcttcgg 12000 gcatgggtag tagataggtg aacatacaaa tacggaaaag tttctaccct cacacgtagc 12060 taagcaagtc atagctatac cccacaaacc tttcataatc actgtatcgg gctatctaag 12120 agccaatggc atggctagtc gtctatggca gtttgtcagt taggtcaaga tgcggtaaac 12180 tgtgtattgc ggctcctatt gtcccgactt aaactgcctg atgttataac gaatacacag 12240 tggttaattg tcatttgctt atgcactgct acgccttgtg gaggccggtc cggcgcgcga 12300 agcatataaa tgaagctcgt cacatccaca tagttgtatc gtacgtcggt gtaggaatcg 12360 aacttggcct ctaattccac atgcgtgcgc tgcaagtgta agtgtaccaa atctaaggac 12420 cacgatgggc tttgtttcag gtatccaaaa ggaaattctg atgccgcgct tcgattcact 12480 aaagtgtgga tgttcccttt atacgggttt gatactcttg ttgtagcagg catctttgtt 12540 agctagcact atccccaacg tataacagtc tacttcctcg gtgtgggccc ctcgtcaggt 12600 gcctgccgtg gagcacgttg aagccgattg gtataataga ttt 12643 <210> 340 <211> 6911 <212> DNA <213> Artificial Sequence <220> <223> SIRV2 <400> 340 tttatcttgc gtatgggcct acgctactta cacgatacct ggtattcgta atggtcttac 60 caagcgtctg ctgaaacggt attccgaggc gctgcgttaa atcgctatgg tgtggttgtt 120 ctcattagtc ggcaaagaga caattatagc ggctaggaac gcaactagtt ataacgaacg 180 gcctcgaata gtagaaaata tccctcctcc gggcacctcc tgaaatgcca catattcggg 240 ttattgaagg cggttaccac ggcagtttta tccagcctca taggtcttta ttccggggtt 300 actcttaccg gctgtgacgc gtcgtgtccc ggctttgagt gcgataccac cggattgtct 360 ggcttggttc accttttgga ttcctcatcg ggttatacta actgacactt attctttaag 420 taaggacccc ttattggcag tggtgagtga gatttggcac tatccttatt actgatgatc 480 ttacccctgt cgcatgaatt acagtacgag cttactttac caggatacca cttgatcttg 540 tggaggatac tcttccaatt cttgaactgg acccacagag atgagtgtac tagatgggcg 600 tacgcattat gtgcccagtt gtataattgt gtgttttcaa ggaaggaggt atgcgtcaat 660 acgttcagta tcgacataag gtatttggat agtttcagtg acatagacca atagattcca 720 gatctcctta cgcatcagta aataccttta tagagtcgat tcacatcaac gccattttac 780 acacatcgcc gacgacaaaa gggctgaaag tggcactcta ctaatttgtt catgtgtgaa 840 caataaacaa tattcgagta cacgttgatg ggatagtgct ctcgccaggc cgtccacttc 900 gtaattatcc tttgagtagt gcaggcgcat taatttagca tgtggccagc atcttagcgt 960 gaagcgatgg tcaaattgta cgcatgccac gttgggatgt caatctggca gattagatac 1020 agctatagag cgaatcacca cgtctcctta gataactggc aaatcttcac aattacgcaa 1080 gaccatagaa tagtcctaag caacatttgc cgcatcgacc aaatcatggc tttgcactcc 1140 aactttgcct gaatcgtgtc agactatagc gggacgttcg accatactac tcattcgatt 1200 acgcattcac ctgacgagaa tagccattct tcctgaacat acgttagaag tagttggtta 1260 atgcttgtct acatttgagt gcgaagccac taggtttgca ctgttgacca ccagcacgca 1320 atcgcaagat acgcagtaat gctaaaccat acttatttga ctttgatgtt ctatcactgg 1380 gttaatgcct gctgctacct gacttttagt cgcgagcaca acgcttcttg gctacacata 1440 tgctgttggg aatgtttttt acacttatta tgctataaat accactccca cagctattcg 1500 aatgactcta atacctcttg caacttggac gaccgtagta cgcattcagt ccgagtacca 1560 agataatgct ctttcttctg ccactcgtct aacttcgtcg gctccgtgaa caagcattct 1620 tcctactagg ccatggcgtc taccgcttct tacatggtaa cctggaggat atggtgcgca 1680 catggaaagg gccgggaagt aactctggcc ttcacagaac aataagttac aatttgggaa 1740 ccaaatcttt gttaaccact tctatacctc gttctaggcc tacgtgaaac ccgaaccgaa 1800 ctcagccttc tttcaaagct gggtctgata cgtgctctac aagtgccgta atcctgaagt 1860 gctgggaacg ctcaaatcag tagtcttctt ctctggaagt cgcgaggggg ggattttatc 1920 atgtcgaagt ttgttattgg actcattcaa ccataggctc catgatactg aaccatcttt 1980 agttacagta ctgaccaccg ccatagggag caccagcata atgactttag aaaccattat 2040 tgagtagtcc tttttgcagt gatcctcatc aaatgcggat acaatcaggg gttaatatgc 2100 gggaaatggg cttagggatg atagacactt tacaaattgc gcaagcgatc gagatgtttt 2160 atgtatcgat atagtatgca ttcttataga attgcctcct agcgtgacag agacaagcct 2220 atctattcgc tccttcatcc tcccatacca cgtgcgtacc tttcccggcc attcattaag 2280 accggaagta ccttgctata caatattaaa cctttaacag cctatcgcgc tgatacacga 2340 attaaccaaa tgaagtttag aggatcaacc actgtcattt ttaagcggct tcttcaaggt 2400 cgcattcata taccaatgat gacttactaa ggttctaaat gagagaattc aattaatctg 2460 gatagacaaa ggggtccgaa gattcataac gccagccact aaatgggcgt tcctctgtgt 2520 acagtaagcc tttatttttt aacctttgaa gaaaccgaca acacgaccct tgcgagtaca 2580 gttatgaaaa gaagagatct tcatctttgc ccccctataa tagtacagct tcaagcaata 2640 ttctcagtaa gatggtacgt gtggtactaa gaaccgtgct tccttctttt tgcgttttaa 2700 cgctacttca attacctacc ataattattt tgccgcccaa gttgtcgcca accaaagcgt 2760 tttctacatc gtttctttaa ttgcaagaac ggatgtggaa tccttcgcaa cacaaccact 2820 tctgccgtgt aatccgagag tggccacgat aatcgaaggt atggtcgacc gttgtaggta 2880 ccgcgtatga aacgtagatt ctagttgaaa cgttaatagt tgccgttgga ttgaatattt 2940 ctcctcaatc gtattaaccg tttcttcttt gtttatcttt gtcggcttct ttgagttcag 3000 ctattctttc cttggaagtt tttcgccaaa aacaacagac agaacctaaa gtcgctttcg 3060 tcctcagcaa cttcgttttc tttatacagg ttgtaacgta gataccacgg taaccagact 3120 aaaccacgtc taatttctga cccaactact ggtctaactt catgctcatg agtgcagtga 3180 caacttacgt ttgttaccgc cgaatggtaa ttgttactga gcacggctag aacgttaaca 3240 ctttcttgca caactctcac atgactaact cccacaactt gcatttgata aacgttcaaa 3300 gctaggacat tttcatggac tagcgctgag caagagacta gttcgacgtg cataggagtt 3360 tctgcattga attcctaatt aacgagcctt aaactctttc atttgctgat gagcattatt 3420 gcgagactag cttgagctcg tattgcccgg tgtgtactat tagaagtgaa aaaatcaggg 3480 ttaatgtctc gtcgcctcat ttacatacag atggccggtg acacctagac gtatactagc 3540 aaaacgacat cttaattgaa ttatttatct tacacattct tcctagatga catgaatatg 3600 ctggtccgat gtataattga tgttccgata taggataaca aacctgaaac gtccacgtca 3660 attacctttc aagaatgtct tgtgtcgacg tcgtcgtgca gtaggtctaa atccagcgaa 3720 tgatttgttt tggcttgttc cgatgtaacg actagcatta cgatcgaccc aaccatttct 3780 tgcagcgcag tcaaagtacg ttccaccatg tttacgctaa ccatcctgta ctttgtaaag 3840 gaactagagg tcctctttat actctgtggt ttgtgcagct cttaaatgca aacgacgagt 3900 aaacaggtgc gtgggtgtat ggggaccttc atttttatgt atgatcggat ggactacgat 3960 cacaaccact gtgtatgtag atcacaatga cgttgtcgtt tgctttaaca ttgtctcaac 4020 taacgttcat agcgttgagg tggacgtctt caatgaaaaa gtagacaacc actacatgac 4080 ttcaaatggg gaagctgacc cgtagttcag tagtaacttc ttcgatgttc gttggatcat 4140 gtttcgatac tcgtgtcaca ttgtccacgt cttcgaatgc gtcgtcaact tctacttcgt 4200 ttgcttctca agatgaaaca ttgttcactt agagcactaa attgtaagca atttagcgaa 4260 cgactacgac agcttcgatg tctcttatca ctattattct tcgttatggc acaacgtagg 4320 aggcgtgata gatgtgacag acatgactgt ctaggacatc gtcgtctact ggatgaacca 4380 tttgaaatgc ttcgacttat atgggcatgt tgcccactta ccgtactggt tcgactatta 4440 tagaagggtc ttgacagtcg aacacacttc catccactcc taattcttga ccttgtgcat 4500 atataacatg aatacgaaca tgttggattc agaagttata gcaaatccca ccctaaacgt 4560 ttggggaaag gctttagcat tatatcctag cctgttggaa cacaagcacc tttcccatgt 4620 aaacccagcg tcttcaccac aatgaatact cccaccgatc gttattgctt ttagtacatg 4680 atgacaccat aactgtgtct gtcgcaatgt aaatgggggt gacaagtttt accatttggt 4740 atgttttagt ttacacatca caaactattt caactaaact cgctacaacg tagtaaactc 4800 ctagtccacg cacccgtttt cgcttcgatg taaagattca tgttagtcta tctgatgcta 4860 atctaatata ccctaggcac gccgacagag ccattatgtg agcctcttac tgttaggtta 4920 ggtgttgttc gatactgggt gactggcatt acctcgtatt gcccagagtg cggtatttaa 4980 aacggtcaaa ttaggccctg aaagttgtaa cgccatctgc acattgttgg ctggtgaccg 5040 gtaggtgtac aataatataa ggtattcaaa attgaattaa atatgctttg cgcccgtcct 5100 acatgagaca aacacagtgg aacgatgcat gattctgcat tcgatccagg ttatgagcgg 5160 gaactcgtat ttaaagtttt tgtcgctcaa aaacctgagc cttcaacaac gttttgggtg 5220 ggacttgcga accccttgcc aaagtcgtaa tataggattg gacaaacctg tcttcgtggt 5280 aagggtgcaa ttgggacgct gaagtagtgt aacttatgag gaaggctaat tataacaaaa 5340 actaaattcc agtgaggtgg aagattgtgt tttaaagatg actaataaat attgttgtac 5400 atgctcctaa tcggttctac ggctcgcggg caccaaaacc catgacctct aaaatagagc 5460 tgagtttaga cgcgtgattt cacttaagat ttcaatctgt tcgtctattg tatccagaga 5520 tatactgcat cccctattag tctatcgatc tcgcatacaa tgactcagac aaagaactca 5580 ctatggaatc cgaggatcgg gcgccaacga tcgttccgat cagaacatga aagctatgtg 5640 gcctgacgaa tacttctctc attgtcttta gataaaagat cccgctggaa taggatccag 5700 tactttgtaa agcaggttga gaatcttgta atgttttgaa gatcatcgga ccaatcacgt 5760 cgtgtgtcga taaaggcatg tcgatgactc ggcatgatat gctaccttct acaattattg 5820 ctgtttcggt agggttggat atcattgcgt atatttcgaa atgtcgttcg catccatgtt 5880 catccaccac gtagcggata aatacaccaa ctacctggtt gtaaattttg cagcgttaag 5940 aacatcaatg gatcataaaa ataaaacgtg catcctctag taacaaaatc taagatctag 6000 acaaacttcc aactagtctg aacttagatt aaagaggttt agtaggtaca cggactctgt 6060 cgtatatatt actttaagtg aaatgttttc cagtgttgat caatccctga atagcaaacg 6120 attataatga gactagagtg taaacgttag cgacgaccga gaggttctta gcggcatcat 6180 agttacctct cggctctagt atctcacaaa tggtcgccga tctgatagat tgctcgataa 6240 tctccgtgtg ctttcgtctg tatggcggac tttataaatc tagtatcagt ctggtttaaa 6300 cgatgctgag cataagtgtg cttgaaaacc gccacataaa gggtaaattc gaaaacaacg 6360 agtgtcctgt gaaccatatg cataactaga gtaccccgca cgctagcatg acacatgcct 6420 ccgcaaggct attcttcgat acagtataaa tagattcatt cgcctatttt tctgtttata 6480 tgcgttatat ttacgttgtc tttaattacc tgccagagtt aaagacattc cattgcgtgg 6540 gaccgggttt tcagtaattc gctatattag cttgaagacc agttgggcaa gacccgacaa 6600 gttggttgtg aatgcccccg aataattctc ctaaaactat gcctgggtgt tggcagcagt 6660 ccattgaaac ttcaggcaga aaggcctagc cggcttaagc gagtggaaaa acttagaagc 6720 gtcctgtatt gattatcgag gaaatacttt ctcatttgtg acggatttcc acctcccggt 6780 tctcaggaag gttaaaggtg taattactat tgacgtgact gcagaggaga agagatcttg 6840 ttttcatttt ttggcggtaa cgtgatcctg aatggcgctt atgacggagc aatgtattta 6900 acgtcggagt c 6911 <210> 341 <211> 10943 <212> DNA <213> Artificial Sequence <220> <223> SIRV3 <400> 341 aatatttcag gcggtatgtt tactttcatg tacctttaat agctcgttag atagtttagc 60 tgctggtaaa cactctatga tctcggctct ccattgatac tacgacgatt attagagagc 120 cagcaacaat tgcaaatgtc agatcaaagt aatattagca aacaataagt ccctaactag 180 ttgtgacctt ttgtaaagtg aatttcatta tatatgctgt ctctagcaca tggataattt 240 ggagaaatta gattcaagtc tgatcaacct ttaaacagat ctagaatcta aaacaatgat 300 ttcctacgtg caaaataaaa atactaggta actacaagaa ttgtgacgtt ttaaatgttg 360 gtccattaga aacaccattt aggattacgt taccctaaaa aaaagatatc aggaactctt 420 ctcttttgca gtcaggtcta tagaaactac atcattaacc ttcctaagaa ccgggaggtg 480 ggaatctatc atatatgaga agatatttgc tcgataatta atactctagg tttctaactt 540 tttccattca cttaaaccga cttaactttt ctgcctgaag attcattgga ttgatatcaa 600 cgcgcaggca tagttccaga aaaattatcc agaagcaatg acaaccaaca tttcgggtct 660 tgcctaatcg gtctacatgt tgatataata aatcactaaa aactcagtgt tacacaatgg 720 aatgtcctta attctagcag gtaattaaag ggaacgtata tataacgtaa aaaaattgga 780 aaattaacga gagaatcttc tttctgtcta ttgaagaatg gctatataga agcatgcgtc 840 atgctagcgt acagggtact cttactatcc atttgggtca caaaacactc gctgttttcg 900 aatttaccct ttatgcgcca gtattgaacc acgcttatgt ctaacatcgt tacaaccaga 960 ctgatactaa atatataatg tccgccatac aaataaaatt atgacgagaa gcatttcaag 1020 aaagcgttgt agcttaaaaa gaacgttgcg gtgctaaagg agaaagtcga aaaagcgaag 1080 gagttaaaac aaaagccggt tattaaatgc tggaagaagg aagaacaaca actaaacaag 1140 gttgtcaaga gcattaagga agccgtacta tgttacattt cttcaacctt ctaattgcac 1200 ccttaggacg aagacagata tgttcttacc tatatttcat cattggcaac gggatcgcat 1260 gtcccacgta aaacattgtt aaaccctcag gtttttgagc gacaaaaact ttaaaaagaa 1320 attcgcgctc ataacttggt tcgaatacag attctagcat cgttcgtctg agtttgtttt 1380 atataaaacg ggcgcaatgt ttattttaat caattttcaa taccttatat aattgtacac 1440 ctaccggtga ccactcaata atgtggggtt cccgttacaa ctatgaggat ttattaagac 1500 cgatttatat tcggcattgt gggcaaaatg acgtttcaac aatgcccaag ttgacattat 1560 tccaaaacgt ttcgtagaaa tgaaagcgct tcgagtaatt aggcgcaaac ttacaggacg 1620 ccatggcata tgggtggtag aaccaacgat ggaagcgagt agcgcaatgc cttagcgcac 1680 gcctacacta actgcttacg aagttggagc aaaagaaact gaacgtgtca gccaaaattg 1740 ccaagaaaca ggagctacca aaagctagtc cggtgtatcg aaagtacaag actagccttg 1800 ctagtaaccg tggactagga gcctaaggta tcactcaaga tacagactca gtaacatacg 1860 ctttagctat ttaattatcc tctatgtctt atagagacct actggccaaa caacactcgc 1920 aaagaattta tgtgcgcttg gcaggtggag aagcaaatac ttggataccg aatttagagg 1980 ccataggtta tggaaaaagt cagtggtatt taggcgtaca atgccttaaa gaataattaa 2040 aaaaaaagca ttccttgttg cctagaatta cttaccgcgg tttaccatac cttcgattat 2100 cgcgcccact ttcccattaa tcagtagaag tggttatatt gcgatagtcc aatatgatat 2160 tctaaggtat tacgttgata aatattttac agagttacca taggcgttga acactacaca 2220 gataatacga atttacgtat agagcgaatt attgaaaagt tatattctcg taattaacat 2280 ttagtccaac tctatcagta cagcagtgcc ttgaataaca tactcatcat taaattttct 2340 ctacaatcaa acgaccaagt acattttcaa gaagtgtgat ggagatttat tcttttgcca 2400 acattgtaat aggcactaaa agaatgatga taattatgag tactgtacta agatgatgtc 2460 gaaacaaagc ggtcttatgg tcaatcatat ttcttttcga gtttcgtcca gttgagtgta 2520 tcactttcaa tgtacttgca agccaagatg gctgtgctta gagtcaattg catgtagaat 2580 gatctccaga catcggaaca ctagttttta tacttaaagc ataaacgacg aacagtcatg 2640 aaagtcttag tactagacgt accattttac tgtgaataat acttgaagct gtactattat 2700 tgcgctgcat agatgtagta ctgttcttat catatttgtt ttgacaacag tcgtcttcac 2760 agtttcttca aacatttaaa aataaacgct tattgtagac agaggtacgc cctattagtg 2820 gctgcggtaa aatatcttcg gatccctttg tctaaccaaa ttaattgaat tctttcattt 2880 aaaaccttaa tatgtcatca ttagtaatta aatgccactt tgaaaatacc gcttaaaaat 2940 gtctaagatt gatccactaa agttgtttaa aataattgct aaatctgcgt gatagggaat 3000 ttaaagttta attttctatt gcaaggaact accgatctta atgaatggcc ggaaatggta 3060 tggaagttat aagcgtggat gaaagggtaa ttagatatgt ttatctatgt tacgctaaca 3120 ggcaattcta taagattgca tattacatca attcataaaa tatctcaatg gcatgcgcaa 3180 tttgtaaagt gtctattatc cttaaacgca tatctcgcat aataactcct gaatatatcg 3240 gcatttgatg ttacctaggt tgagttagtg ttgagctcac ggaatttatt gtatgagtag 3300 tgatttgtaa gaattgttag ttagctcgtt caagtaatag ttgtctacac aacgtcaaaa 3360 taagaaaatg gttgtaacat tatccgtgat tttcttacta ctatcaatat tcatgatttg 3420 actctgctgc agctacgtat cgcctgaaaa ccagttagtg ttaaggaata ctctgagtag 3480 gacaactcat gtagtaaaag ttatatgttc gttgggttct ttcgacacgg atctgagttg 3540 accaacgtct catttgaggt ctgtactcta atgatgagaa atatgtatct tattcttgca 3600 gcttgtcagt actttcagaa tcatggtgtg tatggtagaa tgactcttat aacaaacttc 3660 gacatgataa taaccccccg tttctacttc tagagaagaa aagtattgac atgagcgttc 3720 ccagcacaag agctaaagaa gtttccaatt ttttatttct gaatgacatg tgtctccttg 3780 cggataaatt accaaccgta attcatagaa gcctgggaaa acagataggt ctaattaact 3840 taagagagta aatcttggaa tcattcagta ataactataa acttacgtta gaacttcttc 3900 ggtgaatttt tacagttatc aattaagaaa tttgaagtaa atcaattaag gatttagtcg 3960 cgctatccga taatttccaa attaaaacat attgttctat gaggtacgaa ttacgaacac 4020 cttaacatta gggaaactta gatagttgta caaagtaacg tataacaagt atcgcgtatg 4080 gtatttgttc tgaatcgacc aaggacacaa tcaaattccg atttgtatct tcaacaaact 4140 tgtacccaac ccccgaagtt tactagcttc tcagatatca tagagcttgt ggttcatcgc 4200 gtccgatatc aaactttgtc atgataaagt tttcccctcg ggaataccag agaaaatgac 4260 tactgaattg tgcaatcctt gcactttaac taaggaagct accaatattt agtttttaag 4320 tcttacgtct gacttcgtag atagattgcc atacatagag cttatgaatc agcgaaaagt 4380 atgaggcgct tttaagtatg gcgagtaagt gatttaacgc ttcggatatg actatatact 4440 taaatttgat ctcgtcccga gaattttaaa cctcaacatt tataaattat aaggttagcc 4500 aaaaaagcac gtggtggcgt ccatcgactg ttcccaaact gtagctcttt gttctgtcaa 4560 gatccaagga ttagatagta aaagtcgaaa tctttatatt cggttcgatg tactacgtta 4620 ttcgcgactg ttctgtcatg atagtttttt atatagtcct aataaaagga gtttttaaag 4680 taaatgacat ttcagtagta aaagtttaag agctttttgt tctgccagac gttctgatag 4740 ttgaggaaaa cgtgtggtca gctaataact tgtggtatgg tgtctgctat cgagtccctc 4800 taatgactgg atagttagac ttgcccttgg aaaacatagc aagaccttgc ctctctattg 4860 atattacggc gaatgttgga gagacagcag cgattgcaaa tatcagatca gagtaatatt 4920 aacataggat aactccgtaa ctgattatga ccttttctaa actttacttg attaaatatg 4980 ctgtctttgg cacatggatg atttagagaa attacattca agactgatta agcatgaaac 5040 aattcttgaa tgtaaaattg ttatctccta tattcaaaat ataaatacta gataactaca 5100 ggaattccga cgttaaatac attactccat tataaacgcc attcaggatc acgttaccgt 5160 cataaaatga aaacatgatt tcttctccac tgcactcacg tcaatagtaa ttactcctat 5220 aacctttcta agaatccgga ggtggaaatc tgtcacaaat gagaatatat ttcctcgata 5280 atcattatag aacgcttcta agtttttcca ctcggttaaa ccggctaggc ttttctgcct 5340 gaagtttcaa tggactgctg tcaacaccca ggcattgttt tagaagaatt attcgagggc 5400 attcacaacc aacttgtcgg gactagccaa actggtcatc aagtttatat agtgaaatac 5460 tgaaaaccca gtcccacact atggaatgtc tttaattctg gcaggtaatt aaagacaacg 5520 taaatataac ggatataaac agaaaaatag gcgaatatat ctatttatat tgtatcgata 5580 aatagctttg tggaggcatg tgtcatgcta gcctacagag tactctaatt atgcatatgg 5640 ttcacaagac actcgttgtt ttcgaattta ccctttatgt gacggttttt aagcacactt 5700 atgctcaaca tcgtttaaac cagactgata ctaaatttat aaagtccgcc atacagacga 5760 aagtacatgg agattatcga gcaatctatc tgatcgacga ccatttgtgt gatactagag 5820 ccgagaggta actatgatgc tgctaataat ctctcggtcg ttgttaacgt ttatagtcta 5880 gtttcattat aatcgtatgt tattcaggga ttgattaata ctggaaaaca tttcagttaa 5940 agtaatatat acgacagaga tcgtgtacct attaaacctc tttaatctaa gttcagacta 6000 attggtaatt tgtctagaac ttagatttta ttactaaagg atgcacgttt tatttaggag 6060 taaacgaaca tccaacagta tgtacaatcg tatgagaaag gacaaatctg aagacgatat 6120 tagagggagt ccaacaagtt tgtgatgatt cacgtaagga aatcgaaatg taagattatt 6180 cgtcaatgga ggtataagac aacacaccaa gtaattatac gcgacgagtt ttcatgttga 6240 cgatccctga gactatatca tacaaaattg acccaaataa taaactcgtt ccaatagttg 6300 aagtgttgat taacacattg agacaagttg tccgtacttt aatcatcaaa gttttggact 6360 gacctataaa tgaaatattc gcgttgtctt cagtttaaga agtgatatta ttttcggttt 6420 caagcctatg tggagagata caggaaatag gtaaactata actaatataa atatacgcag 6480 acgaagcctg ttaactttac caaatccaaa tagcgggaga cactaaactt acaagaaatg 6540 tacggtaaga cagggaaaca gggtttgaag tttggaggta caaaatctta atccttcaca 6600 actgaatcaa catttacatt cggttacgtt cggggtttgt agtagtaatt gttgaggtaa 6660 ttttaattgc acttacagac gtggatttgg tgaaacatta tgtggtagat tcatatttcc 6720 gaatcttaat agacgatttt atgatggtga tcgtggtttc ttttcaaggt ttggtggacg 6780 aaaatgaaaa aatcacaatt taacgtttca tattcatcac aatccgaaat cgagatggag 6840 agaaagtttg actaaacttt gttacagtat ttttgaaggc aatcaacctg gaactttgga 6900 tttcatcaag taaacaccca tatttagcga aaatgcaagc taactgcgaa ctaacaaggt 6960 aactcttttt cgatttcaaa atatggtcag tagcaatatg ttaagtgatc cattttggag 7020 gggtggggat ttgttcaatg aatgaggcaa taaaccaccg caacctctgt aattgacgag 7080 aaacttgtat aaggtcatca aaaagaatag atgaacataa agattgaata cggctgggtg 7140 aggttaattc agatttccca aattatgtag atgaaaagat ggtaagttta ttgataggta 7200 aaaaatcaag agagggtaat aaagaagaaa gaataatcaa aagatagagc atgcaaacat 7260 tacatggtag tattgttata ggcggtatat tggtaaatga cgatccttga cttagttttt 7320 tacatcgaac ggttgcaaat cctagcatac ttgatattaa tagtagatac tgaaatatat 7380 ctgagtatat caagggaaat cgagatgtaa caataagtcg aggaaattaa ggtcaattta 7440 gtgcttatca tagaaataac tacttaagcc cttaacgaac taactcaaat taacgaggta 7500 gaggttattc tgaattgtac gaacgtagtc gaagtttgtt accaagggta aagccaaaac 7560 aaaacgattt gacaagagaa tctattccat gtagtagtgc gtttgtacga cgttctattc 7620 gatgtaaatc attaggacga gggtttgaag caactcggaa agatggtcgg ttagaattta 7680 aaagtactga acggaattaa cgaagttggc tactacctaa tagtctttgc ttaggattta 7740 gtagttcgca attgggtcaa agtggtcgct ttggtcgtcg acgtcaagac tcaacaagtc 7800 atttaccgct gtacgaacca tctcatttaa caggttctca accaaatagc ggtaggagta 7860 gaaagcactt accatcgtac gaactttgac aacgaaaggt tcaaaggtaa ccaaggcgta 7920 gtagtgagtt aggtcaatgt ataaaaactc cctttcttat tttgagtcgt caatgtagtc 7980 gagaaagtgt ccaagcaaaa cgtaacgaaa cggttgagta aggacttgca cactatgccc 8040 ttgcgggttt acctgcttga cgtagtcctg ccacttatta tccgtgattg ttacctaatg 8100 gacaacctaa tcgttagctt aaagaagatg cacttagtgc cattattatt agacaagata 8160 gtccgttctt tagtcgaagt tcttgttgta ataacaaaag tagagcagta ttttgacgta 8220 tagggagaca gtcattaggt cattaaccca gttactaagg acaacgtaac catcgaagtt 8280 attgaacttc aggtacctaa cagtaaaaga gtcacataat gcctctgact gcgtgcctag 8340 ggtatatttg attagtatca aatagactaa aatgaatttt tacatcgaag cgaaaatggg 8400 tgcgtggact aatcaggagc aaacgaaaaa tcttgacctg tttgatgtct cgtaatattc 8460 ttagagatga acgaaatgtt tcatgaccta gaaaaaagtc gttctacaaa atagatttat 8520 gttactctct tcataaagaa ttcggtgtac cgaaagaaca aagcgagttt aggtagtaac 8580 cgttgactat gggggtaaag tatcactcaa gaagcagact cagtaacaca cggtttagct 8640 gattgtttat cgtttaggtc atatagagac ctttaatatc tgtatgtcta gctttaaaat 8700 ttactttagt gcgcaaattt gagtcgaaat aaaatcacca gtacccaaaa ccaaggatta 8760 cgatccgaaa tttcatggac acagatattt gagatcaaca tttgattcgt tgacgtattt 8820 atgttgattc ggttagcgag agttacaaag ttagtaatag aaagaaagag gaaataataa 8880 tcaaaagatt aagcatgcaa acattaagag gacacatatg tattggtggc atttgcgtta 8940 ggttaacgtc tcgtaagacg gtgaagctgc ttaaactatt cggactaaaa tagtgaaatt 9000 tatgatgtgt gacccgttat gctccatttc gatcagtggg ttattactag tagtcgattg 9060 cattgtcatt ttccgagtga tttagcgtga cagtcgcaga gaacccataa aatgtaatcg 9120 tagtccatct aatcatactt aaaaatgaag gtctcctttt acccacgcac ctgtttactc 9180 gtcgtttacg tggttaggtc gcttggtagc atatctgtta ccgaagacac gacagtaaat 9240 tgtcaagcaa acactgcgcc ttcttcggtc gcataaagaa gtgaaccata acgttctcct 9300 acttaactcc gcaacgaaaa agagctattg ctaatccaat ttatctcatg aaattataat 9360 tatatagacc gaaattttaa atcaaatcac ataattaggc tcagctttat tttagtggtc 9420 atgggtttta gtaccattcg ttcaacatga agtggcttat gttcgcggag tagtagcgcg 9480 taaaagtgta ggtacgcgag ctatcgtgcg gtgtagcatc gcagtcaaag cttgcgtagg 9540 tagcgttgca atctcctgta ctaaagacat gcaactgcca gcttgtactg ttggcgaaga 9600 aagttcagtg gatgttacta accgcgttta acgcgtggac acggcggcag ttgagccaat 9660 cgattacgaa acaagctatg ctaatgttga ccatttggct cttcgaccag cagtcagccg 9720 cttctttacc tgccaacgtt gttcataaca ggcaaaacca gaaagtcgca taggttggtc 9780 aactttcgca gaaccaactg ttactgcgat cttcagcttg ccttttgtcc cagattaacg 9840 tgtaattatt aaacaaagtt cggcagttcc agtcgctatc gacaaaaccg aaaccacaag 9900 ttctgtaatt acgcgttcgg caaagcgcaa tgcgccctaa tacttaattt ttttttcgta 9960 aggaacaacg gatcttaatg aatggcgcca aatggtatgg aagctaatag cgcgggtgag 10020 agggtaatta gccatcttca ccaatataac gctatcaggt tatactataa gattccataa 10080 tgcaactatt tataaaatgt cttaatggta tccgcaactt gtgatgtgtc tactatgctt 10140 aaatgcatat ctcgcttaat aacttttcaa tataagagca ttaattgtaa atcaggttga 10200 gataatcatg tcgtcacgga acttattgta agagtaataa tttaaaagag atgttagttt 10260 gctggttcac gtaaaagttc ctcacactac ctctaaataa gtaaacggtt gtaacattat 10320 ccctgatttt cttactacta ttaatactca tgacataatt ctactacagc tttgtttcgc 10380 cagaatacca gttagtataa agaagagctc aaagcaggtc aactcgcatt gtgaaagtta 10440 catgaacgtt cggtactacc gacacgaacc tcagttaacg tacatcttac tagaggtctg 10500 tggccttgtg atcaaaaata tgaatttcgt atttgctgct tgtcagtact ttcagaatca 10560 tgatctgcat ggtaaaaaga cacttattat gaagttcgac atgataataa cgcgacgaat 10620 ctacatcatg acaagaatag tataaacaaa actgttgaca gcagaagtgt caaagaagtt 10680 tgtaaattat tatttgcgaa aaacatctgt ctccatgggg gataatcacc gacgccattt 10740 tatagaagcc tagggaaaca gattggttta attagcttaa gaaagtaaat tctggaatta 10800 tactgtagta atcattaatt tacggtgaaa gttttatggc gaatttttac aaattcaaac 10860 taggtgattt caacaaattt tattaacgat ttagacgcac tatcccttaa attacaaatt 10920 aaaaaataac gttccttgat ggc 10943 <210> 342 <211> 16122 <212> DNA <213> Artificial Sequence <220> <223> SIRV4 <400> 342 acttattgtt tgctaatact acattatcct cacaataccg aatgtagttg gctctctaaa 60 aatcctcata gttgcaacgg ggagccgaga ttattgagtg atcaccagta gctgtactat 120 tatataagct attaaagatt gatcaaagta aacataccgc ccgtattata taaatcatac 180 tcagacgaac attgctacaa tccgtattcg caccaagtta tgagcgcgta tttcccatta 240 aagcttttgt cgctcacaaa ccacagggtt taccaatcat tcacgtggga catgcgatcg 300 tactgccaac gatggaatat gggtaagaag ttatctgtct ttcttctaag agagcaatta 360 aaaggttgaa gaaatgcaat atatatgcaa gggaaattaa tggacgatct taattcctgt 420 aaggtagcag attgtgactc aaagatgact aaataatatt gttgtacatc tgcctaatcc 480 gttctgggct ttacgaccaa cagtaaccga gacctataaa aaagagcttg atacggacgc 540 gcaactatac ttaggatacc tagatgtccg tcttttccat tcagccaaat tctgttaccc 600 ttttcaatct acggatctca taattaatag ctcgtttata gaagagtata cactatctaa 660 gggtgggggg ccaacgatcc ttccaatcag aacatcaaag atatctggac tgacgttttc 720 tcctctcaag gactatagaa aaaaaatccc gctgcactag gatctaccgc taagtttacc 780 tggttgtaaa tcttgcagtg ttaagaagat caatggatca taaaaataaa acgtggaccc 840 tttagtaacg aaatctaaga tctagacata tttccaacta gtctgaactt agattaaaca 900 ggtttaatag gtacacgaac tctgtcgtat ataatacttt aagtgaaatg ttttccagtg 960 ttgatcaatc cctgaatagc aaacgattat aatgagactg gtgtagcata gcaactgttc 1020 tgaatgtgat attcggtcag gccacgttga cacttaccaa agcaagacag aaggaagttg 1080 acgggacaat agggaagatt ggcaaaggga tttaaacata gtccagttcg cgtggtgaat 1140 gacagattgc tactgttacc attggcgata ctacgttggc gactttactt gcctcggtag 1200 cgacagaaca agtgtcgtct acgcaacgaa caaccagcgt gttagctgca atgtcccctg 1260 tagaagaccc tcttctttct cttctgaatg atgtagctgt ggatagagcg tctcccacgt 1320 tagggtctag caaccctccg gtggtggttt cgagaacgat ataaaaagtt cccgtacaga 1380 ttgatgggtt agtgttctcc ctgggctgtc gtctgcatga cggaccttat agtagtacag 1440 tcgctttaat ttaaggatcg tggcccagtc agtggattac gttgaccatg agtctggctg 1500 ttcttggttc accattagct gtggtgacaa taacgggcat tgtggcaacg atacgtcgta 1560 ctgcaagtcc cactgtaact aagacaattc ggattcgacc gatacttggt ctggttcgtt 1620 aacttcgcgt accttctgct ttaccagcag gtcgtcaacg acgagccacc gtaacgagtg 1680 tggtttcggt tcgcttggtt gggtgcccaa tttccagtac cgaagaggta attgcaactt 1740 caattgctta gttgacgtga cctttcgggt gtcatggact accgacggta tctcatacgt 1800 gacctcgttg ttgacctcgt tcttcatctg cacagactac aacgttagga ctacggaacc 1860 agtgtgaagt tgacggacgc gctaggttat catcttttat agggaggagg ccggtagagg 1920 actttacggt atataagccc aatgactacc gcgagtggtc ccgtcaaaat aggtcggagt 1980 atccacaaat aaggctccaa taacaatggc tgactcttgg cagcacagcg tcgaaacttg 2040 gggcacagag gcctaacaga ctgaaccaag tggaaaaact aaggagcatc ccaatatgat 2100 tgactgtgaa taagattttc attcgtgggg gatatccgtc accagttact caaaaccgta 2160 ataggaataa tgactactaa aatggggaca gcgtacataa tgtcatgctc gaataatatg 2220 gtggtatggt gatctagaac acctcctata gtttacagat gcggtttatt ggattggtta 2280 caacggcaaa ggcggagacc actccaactg tcggaagagt acctcttcaa attgccattc 2340 caattgctcg tcatggactt tccactctta gacgacccga tgaagctaga agtctgacac 2400 tgaccatgct tgtggcaatc gttgttcatg tagccacttt gactcgacgt tcacgaacat 2460 acgatggtta gttcttctac tatactaatg gcttgttttc gcagaatctg tatttagacg 2520 taacaatgga aataatcttt ttggacattg atctagattc tttaataata ggtttattta 2580 ccaagttgac gtttcacaag ttgttttaaa ccgacttttt ttgatgtgtt tgtagttatt 2640 acagctgaaa gatgacgcgt actagagccg cggcgtgtca aactttgcat gccagtcgcg 2700 acagcgagtc aggttaagac tagcctatgt taatttgcac atgctaacat aagtcaataa 2760 ctttaaccgt cttctttata tgttgttgct gcatgattat ctgtaaccac tatctagtta 2820 ataatatggt tgtaacagtg tctgttacag tcaccatgga ttttgttgtt atggatttgg 2880 ctgttgacac agctgtagcc tatattacgt tttttgtttc cacggttgtt attaatagct 2940 aagacttaga aggagcggcg tttgtgtata cggcggtggt tatggtggtg gttatggcgg 3000 cggtggcggc ggcggagttt gtttgaatag aagctgctat ggaggaggcg gcagcggagg 3060 aggcggtggc agtaatggta attatcttct atatcattat ggtaggtcat ttctagcgct 3120 aatatgttgc agctgagccg ttcaaaattt tctaggtgca tggcttaact accttgttta 3180 tgtctttcct taatttaatt tttttcatag ttttgggcgg ctaccgccaa gctatcattt 3240 atggcaatgg cggcggcggt caggatgacg tttctaaaat gttgctgcgt atcggcaagt 3300 tggctcagga catcaaagca gtcttagcct tttgcccacc tggctgctgc ttgttgtcgt 3360 cgctcggtcg tcgcttaatt ttgttataca tgctagaaac aggttatatt gtaacctgtc 3420 aagtacctat tttttaatag gaaacagatg acttcgtgtt ctgtgtaatt atagttattt 3480 tttagttaat ttagttgccg aaagcttacg tgtttgtgtt gttcaaagac aatttgatgt 3540 ttattaataa cttctattga actatgttgt ttgcatacta ttaggaaacc tgtttaggct 3600 actacagctt gaagttaaca ggctattttt aaatgttctt aagtaatacc gccaccttct 3660 aaactgtaag ttttttgtca tgctatttcg caatcgatta caccacagcc gtaatccttg 3720 gtttggctgg ttagttttta atgttcttaa cgctagctta aatctatttt aatactcaat 3780 gtttaactgc tacagttgac ttagcgttca aatttaccgt gtccaagttg ttgactgggg 3840 gcgagttgtc cgccgtaacg cgttagtcgt ccgataccgc cgtttgaacg tccgggcagt 3900 ttaacaggac gttgtcagaa tgggccaata agtcgtccaa ctttgcgttt gaaagctggg 3960 ccgattggca aggcacaaac tgcactgggg ccgcttgcac ccgtaacagt ttccgaaaaa 4020 ccacttccat tgccgccgat agtttttatt ggtgacatgg cttcgccacc gggaatgact 4080 gaagccgggc cgattggaac aagctatgtc gcgtcgtgtc ccggctttga gtgcgttagc 4140 accggattgt ctggctcagt tcagcttttg gatacctcat cgggttatac taactgacac 4200 ttattcttta aataaggatc ccttattggg agtggtaagt gaggtttggg actatcctta 4260 tttctaatga tcttacccct ttcgcatgaa taacagtacg agcttacttt acgaggatat 4320 cacttgatca agtggaggat actcttccaa ttcttgaaca ggacctagag agttgaatgt 4380 attagatggg cgtacgcatt atgtgcccag ttgtataatt gtgtgttttc aaggaaggaa 4440 gtatgcgtca attcgttcag tatcgacata gggtatttgg aaagttttag tgacataggc 4500 caatagattc tagatctcct tacccatcag taaataccat tatagagtca attcagatct 4560 acgccattta acacacaacg ccgaggataa aagggctgaa attgacagtc tactattttg 4620 ttcatgtgtg aacaattaac aatattcgaa tacacgttga tgcgatagtg ctctggccag 4680 gccgcccact tcgtatattt tctttgagta gtgcaggcgt attaatatag catgtagcca 4740 ggaccttagc atgaagcgat gattaaaatg tacgcacgcc acgttgggat gtatgtgcta 4800 tagactttta gtgttacccg aaacatagtc catcgatttt gccataagac ttcggcacga 4860 agttaagtga tttcacacct acaagagaaa tgtgccgaat ctatgataat atcaccgttc 4920 gttgtattaa tcaataatgg tagcggctgc atattatcaa atgaaggagc gacacccggg 4980 gaggagttca cggtggatat cccgtaccac tatattaatt attccgtaat cctcgtctta 5040 cctttttgct tttatctgga cgtgtttaac attgatgttt gaccagtctt tgcaaaggtt 5100 tatattgtcg tagacgttgt tcagcactat aatcgttagg tcattaacat ctgatgtcta 5160 gtgctaaaga catattatta tgtggttttc tacaacctta tatactaata cagctgtatt 5220 tcttaccatg atgacgtata ccttttacaa ccctttttgc taaattcgga agtttaccat 5280 aaaacatacg actttcatgg aattttccgt tgcaacgatg tagacttcgt cggtgtttat 5340 cattacagta ctgaggatat ggtccaaatc ttttacctat aaaatattta agacgtttat 5400 tacgacttgc attactgtta gtttgtaaac aaggaggatt tcgcataagt cgttgcttta 5460 atttagtttg gttatggtgg gttaatcgac ggatacatct aatataccca atattaataa 5520 aacctcttgt ttttacacgt ggcgttcgtt acggttttaa atactaaccg aaacatttgt 5580 aagcattact gctgttaagt aatgattgac gttttaccct gtaaaattat ttttgagctt 5640 atgtaaacag accatacgtt cgatgttctc ttacccaacg ttcttgcact gcatatggtg 5700 gtgttaccaa gtgtagcgtt atgtgagtta aggcaatggt tttatattta gatgttccta 5760 ataatgcttg cccattatgc cgttttgatg gttgtttctc tggtgtttac gttagattac 5820 atccgtaaga tggttacacc tcactcctac aacggaaata tgaccaaagt atcagaacaa 5880 catggtccgc agaaagaatc gatcgtgata aaggttggat cttgacagaa caaagagcca 5940 cactctggga gcagtcgacg gacgccacca cgtgcatttt cggctaacct tataatctat 6000 aaatgttgag gtttacagtt ctcggggcga gatcaaactt aagtatatag tcgtatccga 6060 agcgttagat cacttactcg ccatgcttga aagcgcctca tgcttttcgc tgattcatta 6120 gctctatgta tggcaatcta agtacgaagt ctgtcgtaag gcttaaaaac taaatattgg 6180 tagctacctt agttaaggtg caaggattgc acaattcagt aggcgttttc tctgctattc 6240 ccgagcggga agctttatca agacaaagct tgatatccca cgggatgaac cagaagctct 6300 atgatatctc agaagctact aaacttcggg ggttgggtac aagttagttg aacatacaaa 6360 tgggaatttg attgtgtcct tggtcgattc tgaacaaatc gtagtgaacc cgtgcaaagt 6420 atacatatta attcatacgg cgtctcaaag aggcaatggc ttgacttgag ctggattaca 6480 gattatgagt aaccgcaaga tagagtaaac actgtaatcc atgcttaatt gagaccactt 6540 aaagatgctg ataccatttt caaaacacga tggtttgttg acaattattt gtacaccgac 6600 gcatcttgcg gttcttggtc gaccctactt acggtataaa tgaagcacgt ttcatccaga 6660 ctcatccatc gtagctccaa atacggattc aacttggcgt ttatatccag aaaagggccc 6720 tagaggtgat agactcttaa gtctttggac taccatggga tatgtttcac gtatcgaaat 6780 agtaaccatt attctgcgtt tgctttcatg caagtgagga tgaagccttt gtattggttt 6840 cttaatcctc ctataccacg cgtgtatctt tcccggccct tcattgaatc cggaagtgtc 6900 ttgttactca atgtcaaacc cttgtcagcc tcttgcggtg atgcacgaaa aaaccgaatg 6960 gaatataaaa tattaactac tcctaatttt aagtgccctc ttctagattg gattcatata 7020 tcaatataga cttcgcaagc tagtgaatga ggggtatgaa tttatccgga ataacaaaag 7080 ggactgagta ttcgagatac gaaccgttag atagatgctc cagtctgcat agtgaatttt 7140 tgatttttaa ccattgaagg aatcaacttc acgaccctta cgagttaagt catcagaaga 7200 agagaccatc agcgctcccc ccttgaaata gtacagcttc aaacaatagc ctgagtaagt 7260 tggtatccga ggtactataa acttctcgac catttgatgc ccccaaccta tgtttaaaga 7320 actattatct ttagccttaa gctaacacag gaaccagctg agtcttgcct agcctcactt 7380 gggctggtta catatataaa ataatgtatg ccgcagaatc tgtcccttag cgaattgtag 7440 ttgaccaaaa gtctattact cattggcgtt gtgtcacttt tgtgtcaata ggtatgatca 7500 atctcttgga aatttttacg acgatggtaa aagattagaa ctactaaaca actgttaata 7560 atcatgtcgc tccgtagatt gccaagaatc agttgggatg agtacgttat ttacgacgta 7620 caagctgcga ctgagttgga ctatgattaa taagaggttg attacatcct gattgcatgg 7680 actgcgatca cgttttgtaa gttcactggg actgttacgc ttaactgctc ggaattcgag 7740 aaaatatacg actgttcgta agagcgcttt gatcgaacac gatcataaca gggcacacat 7800 gataatcatc acttttttag tccctattac agagcagccg agtgaatgta tgtctatcgc 7860 tcactatact atttgcgtgt tatagggtaa cctacgaaag tgaaactact tcgtattgct 7920 cgtgttttta attcgtctcc gattaataca ctatactaaa ttttaaagcc agatatatca 7980 atattaaact actcaattta acctaatcct caaacgttct actaaaccgt gtcaatcagt 8040 gtctgcttcc tgaatgaaac tttttcgcca aagtcaacag ttagaaccta aagtcgcttt 8100 cgtccttagc tacatcgatt tctttataca ggcagtaacg tagatccatt ccgtagaacg 8160 gtttttcact tggacatgaa acatttcttt gatctccagg agaaatatga gaaactaaac 8220 acgtcgagta aatacgtatg accattcctc ttgatcgatc caataagtct ctaatgtgtc 8280 cagagttcca acttcttcaa catacgctct aaatatcaaa tccaacagca aatggtaaac 8340 aatgaagacg gtgttgttca agacatcgtt tgctccacta aaataaagct gagtctaatc 8400 gcgtgatttg atttaaaatt tcgatctgta tgactataat ttcgagagat aaactggatt 8460 agctatttgc tagacgattt ggcacagaat gacacagacg aagaactcac tatgaaatgg 8520 cggtttcagt tgccaatgat ggatttcagc gaaactagga agcgatgtgg cttaagaaat 8580 acctctctca ttgcatttag ataaagcatc ttgctgaaaa gagaaccacc ttgtcacctt 8640 ccacgtatgc ttttgttgta ctggttatta ttgcaacttt acctttgtgt tccactccgt 8700 agtggccatc gtcctcctag tcgtgttcaa ctaggtgcat gtcctccctt tcgtcggcca 8760 ccttaccctc gtccacctcg tcgtttgcta cctgcagtcc tgtataaatc acctcgacga 8820 gtcggattag ttgtagtagt taatactata cccttttcga tagtgaaatg tcattggtta 8880 cctaatggtc taatatctgt gaaacaccgt tcacctttgt taatgtaacg ggttgtttct 8940 aaatttgtat atgtgcctta tggaaccctt gcgaataatt acatacattc ctagcattat 9000 atcctagcct gttagaacag aagcaccatt cccatgtaaa cccagcgtct tcatcacatt 9060 gaatactccc accgattaat attgttttta atatatgatg actcgacctt ctaacacaaa 9120 ttttctactg attatttata acaatatgta ccaggattag ccaaggtgcc gagcgcccgt 9180 ggttttgggt actggtgatt ttatttcgac tcaaatctgc gcactaaagt gaattctaaa 9240 gttagacatg cagatattat aggtctctat atgacgtaga cgataatcaa atagctagac 9300 cgtatgttac tgagtctgct tcttgagtga taccttagcc ccctagtcca cggttactag 9360 caaggctagt cttgttcttt cgatacaccg gactgcttat gaagagagta acataaatct 9420 attttgtaga gcgaccttat cctaggtcat gaaacatttc gttcatctct aagaatatta 9480 caagacatca agtagcccaa gatttttcgt ttgctcctga ttaatccacg cacccgtttt 9540 cggatcggtg taaagattca tcttagtcta tttgatacta tccaaatata ccctaggcac 9600 gcggtcagac gcattatgag actctcttac tgttaggtta ggtgttgttc gatactgggt 9660 gtctgccaat acctcatatt gcccacagtg ccgaatttaa atcggtcaaa ttaggtcctc 9720 aaagttgtaa cgccatcccc acattgttgg gtggtgaccg gtaggtgtac aataatataa 9780 ggtattgaaa attgaattaa atatactttg cgcccgttct acatgaaaca aactcagtcg 9840 aacgatgcaa gaatctgcat tcgaaccaag ttatgagcgc gaactcctat ttaaagtttt 9900 tgtcgctcag aaacctgagg gtttaacaac gttttacgtg ggacatgcga accccttgcc 9960 aatgtcgaaa tataggaatg aacaaatctg tcttcgtgct aagggtgcaa ttaggacgct 10020 gaagtaatgt aacttatatg caaggcaaat tatagcaaaa actaaattcc agtaaggtgg 10080 aagattgtga tttaaagatg actaataaat attgttgtac atcctcctaa tcggttctgc 10140 ggtttacgag caccagaatc caagacctct aaaatagagc taagtttaga cgcgcgatta 10200 tacttaagat atcaagctgt tcgtctattg tatccaggga tattctgtat cccttatcaa 10260 tctatcgatc tcgtatacaa tgactcattt atagaactca gtatagaatc taaggatcgg 10320 gcgccaacga tccttccgat cagaacatga aagctatgtg gtctgacgat tacttctctc 10380 aatgtcttta gaaaaaaaat cccgctgcaa taggatccag cacttagtaa agcaggttgt 10440 gaatcttgta atgttttgaa gatcattgga tcttaaaaac caaacgtgga ccgattagaa 10500 acgtaagcta agatctagcc acatttctaa ctagtctcag gtaaattata ataggtttat 10560 atggtacgcg taccccgttc tgcataatac tttaagagaa aggcaatcca atgttgatca 10620 agctctgaat tacaaacgat tatgatgtaa taggggtgtt atggcttaca tcaagccaga 10680 gatttttagg agtatcaacg ttgcccctcg gctctaataa ctcactagtg gtcatcgaca 10740 tgataatata ttcgataatt tctaactagt ttcatttgta tggcgggcat aatatattta 10800 gtttgagtct gcttgcaacg atgttaggca taagcctggt tcaatactcg cgcttaaagg 10860 gtaatttcga aaacagcgag tgtttggtgt cccaaatggt tactaagtgc accctgtacg 10920 ctagcatgac ggttgctact ttatacccat tcttcaatag acagaaagaa gattctctcg 10980 ttaattttcc aacttcttta cgttgtatat acgttccctt taatttcctg ctagaattaa 11040 ggacattcca tcgtctaaca ctgagtttct actgatttat tataacaaca tgtaggcgga 11100 ttaggcaaga cccgaaatgc tggttgtctt tggctctgga tattttttct cgaactatgc 11160 ctgcgcgttg atatgaatcc tatggatcta caggcagaaa agataagtcg gtttaagaca 11220 atgggaaaag ttagatacct agagtattaa ttatcgagca aatatcttct catatatgat 11280 agattcccac cccccggttg ctaggaaggt tagtcttgta gtttccatag acctgactgc 11340 taaagaggag agttcctgat atcttttttt tagggcgacg tgatcctaga tggcgattca 11400 aatggaccaa catttagaac gtcacaattc ttctagttac ctagtatttt tattttgcac 11460 ctgggaaatc attgcttttg attctagatc tgtgtaaagg ttgatcagac ttgaatcaaa 11520 tttgtccaaa ttatccatgt gcatgagaca gcatatatta tgaaattcac ttttcaaaag 11580 gtcacaacta gttggggact tattgtttgc taatattaca ttgtcctcac attagcgatt 11640 gtagctggct ctctaaaaat cgtcgtagta ccaatgggga gccgagatca ttgagtgatt 11700 accagcagct agactatcta acgagctatt agaggttgat caaagcagac ataccgcctg 11760 aaatatataa atcatagtca gaccaacatt gctacaactc gtattcgcac caacttttgg 11820 ccgcgtattt cccatttaag cttttgttgc tcacagaaca cttggtatac ctatcaatct 11880 catgggacat gcgatcgtac tgtgtacgga ggtgtttcgg taagaagcta tgtcactttt 11940 atctaagtaa gcggataaaa agacaaaaaa acgcaatata tatgcaaggg aaattaatgg 12000 acggtcttaa ttcctgtaag gtaacacacc gtgacccaaa agtcattaag tgatataatt 12060 gtacatctgg ccaatccgtt ctgggctctt caaccaacag taacgggggc ttattaagaa 12120 gattttgata cggacgcgca actgtcgtca ggtaactttg aagtccgtct tttcggatcg 12180 gccaaattcg ctcacctttt tgaatcttcg gatgtcataa ctaatagctc gtttatagaa 12240 gagtatacac tgtctaaggg tggagggcca agaatccttc caattaccac attaaagata 12300 tctgcactga cgtctcctct tctcaaggac aaaagtaaaa aagtgccatt gcactaggac 12360 ttaccgcaaa gattacctcg ttgcatatat tgcagcctta aggacatcaa tggatcataa 12420 aaataaaaca tgcatcctct actgttaaaa tctaagttct agacaaacta ccaattagtc 12480 tgaacttaca ttaaagagat ttagtaggta cacggtctct gtcgtataaa ttactttaac 12540 tgaaatgttt tccagtatta gtcaatccct gaatagcata caattataat gagactagac 12600 tataaacgtt agcgacgaca gagaggttgt tagcggcatc atagttacct ctccgctcca 12660 gtaccacaca aatggtcgcc gatcagatag attggtcggt aatctccgtg agctatcgtc 12720 tgcatggcgg actttataga tctagtatca gtctggttta aatgatgctg agcattagtg 12780 tgcttaaaaa ccgtcacata aagcgtatat tcgaaaacaa cgagtgtcct gtgaaccata 12840 tgcataacta gagtactccg cagactagta tgacacatgg ctccgcgagg ctatttatcg 12900 atacaatatg aatagatata ttcggctatt tttctgttta tatccgtaat atttacgttg 12960 tttttaattg cttgccagag ttaaagacgt tccatagcgt ggaaccgggt tctgggtttt 13020 tcgctatata agcctgatga ctacttcggc tagtcccgac aagttagttc tgaatgccct 13080 cgaataatac tcctaaaaca atgcctggct gttggcagca gtgcattgaa acttcaggca 13140 gaaaggccta gccggcttaa ccaagtggaa aaacttagaa gcgtcctatt atgattatcg 13200 gggaaataca ttctcatttg tgacggattt ccacctccgg atactcagaa aggttatagg 13260 aataattact attgaggtga gtacagtgga gatgatgtca tgtttacatt atatggcggt 13320 aacgtgatcc tgaatgccgc ttatgacaga gtaatgtatt taacgtcgga gtccctgtag 13380 ttatctagta tttatatctc gaatacagga gataacattt taacactcaa gaattgtttc 13440 atgcataatc agtcttgaat gtgattactc taatccatca acgtgtcaaa gacagtatat 13500 ctaatcaagt aaagtctaga aaaaggcata atcagttacg gagttatccc atcttaatat 13560 tactctgatg taatatttgc agtcgctgct gtctctccaa cattcgccgt aacatcaata 13620 gagaggcaag ctcttgctat gtttttcaag ggcatgtcta actatccaat accggttctt 13680 attagaactt cttttacaca atttagtaag gcatatgcct tacccatttt cctttagttt 13740 actttttcta acccgatgtt aattcgcata attagcacgt gcacgtcgtt cttctctttt 13800 agttctttta gttgttctgt atctgcacgt acctgtgcaa cgtccgcttt tattacttgg 13860 tctagtgctt cgtttaaacc gtctaaaagg cctaaaggag gttctgaaac atcgacttcg 13920 tcctggttca ccttggcctc aataatggta gcggctgctt atcatcaaat gaaggaggga 13980 cacccgggga ggactttacg gtggatatcc cgtacaactt cggctaatca tgttatctaa 14040 ataagttggg gtttgcagtt gtgagggccg aatcagattc gactatacag cgcagttcag 14100 agccctaact cacgtaatcg tggcctaaca gaccgagtca agctgataac ccatcgagta 14160 gctcaatatg agcgactgtg aataagaaat ttattgcaag gcaataatcc tgatcattga 14220 ctccaaactt tgataaggat gaagattagc acaattgaga aagcgtactt actgtcatgc 14280 tcgaatgaaa tgctctatta aagaataggt tgacgtccta cgagaaggtt aagaacttct 14340 cctgcatctc acaacctaca taatcttcgg gcatgggtaa tagacgggtg aacatataaa 14400 tacacaaaag tttcttcctt catacgtagc taagcaagtc atagctatac cccacaaacc 14460 tttcataatc actgtatcgg gttatctaag agctagtggc atggctagtc atctatggta 14520 atatgtcagt taagtcaaga tgcggtaaat tgtgtattgc ggctcctatt gtcccgactt 14580 aaactgtctg accgtatcag attaaccacc tgatcctaat tgcctattga cagaacaaag 14640 agccacactc agggagcagt cgacggacgc caccacgtgc attttcggct aatcttataa 14700 tctataaatg ttgaggttta cagttctcgg ggcgagatca aatttaagta tatagtcctg 14760 tccgaagcct taattcacat attcgccacg cttgaaagcg cgtcatgctt ttcgctgatt 14820 cactagctct atgtgtggca atctaagtag gaagtctgtc gcaaggctta aaaactaaat 14880 attggtagct accttagtta aggtacaagg attgcacacc acggagcata agtaaaagga 14940 tttgtattaa tagaaccagg accatcacca ttcaagttac acggtggtca actacttctg 15000 ctgcgttatc gttctgtatt cctaaatcga atggttgttc gacttttagt atttctgtat 15060 aagttcaacc tgttttgacg ctctctgctt aagaagtcaa gtaaatatgt atcacctttc 15120 accatctaag tgaggatgta gcctttgtat tggtttctta atcctgttat accaggcgtg 15180 tatctttcct agcgctttat tcaacctgga actgtcttgt tactcaatgt caaacccttg 15240 tcagccactt gcggtggtgc acgaaaaaac cgattggaat attaaatatt aacaactcca 15300 aattttaaga gccctgctct agtttggatt catatatcag tatagacttc gcaatctagt 15360 gaatgagcgg tatgaatttt cgcggaatat gaaaagcgac tgagtattcg agatacaaac 15420 cgttagatag atgctccagt ctgcattctg aatttttgat ttttaaccat cgaaggaatc 15480 aatttcacga ccctaacgag ttaagtcatc agaagaagag accatcaggg ctcccccctt 15540 gaaatagtac agcttcaaac tatagcctgc gcaacttggt atccgaggta ctatagactt 15600 ctcgatcatt tgaagccccc aacccatgtt caaagaacta ttatgtttag ccttaagcta 15660 acacaggaac cagctgagtc ttgcctagcg tcacttgggc acgttacata tataaaataa 15720 tgtatgccgc agagtctgtc ccttaccgaa ctgtagttga cctaaagtct attactcatt 15780 ggcgttgtat cacttttgtg tcaataggta tgatcaatct cttggaaatt tttacgacca 15840 tggtaaaaga ttagaactac taaacaactg ttaataatca tgtcgctccg tagattgcca 15900 agaatcagtt gggatgaata cgttatttac atcgtacaaa ctgcgtctga gtaggtacca 15960 tcgtagttta tgcctatgtt ggaccgcaaa tatacgtctt ttgccgggat ccccactatc 16020 tgtgaaatgt gaaacctgtt ggttacctat acaaaataca tagctatatc attggtaata 16080 atatgttaac ggaagatcgt tcactctttc ttcggaaaga ta 16122 <210> 343 <211> 14606 <212> DNA <213> Artificial Sequence <220> <223> SIRV5 <400> 343 cctagggtat tttacgttag catcaggtgg actaacatga atctttactc ccaagcgaaa 60 acgggtgcgt ggactagcga ggagcaaacg aaaattcttg gcctgcttgg tgtctcgtat 120 ttctcttaga gatcgacgaa atgtttcatg accaagggaa aggtcgccct acaaaataga 180 tttgcgttac tctctccata aggagtccgg tgtagcgaag gatcaaggcg accctaggta 240 gcaaccgccg gcttcggcgg taaggtatca ctcaagaagc agacacagta agacacggtc 300 tagctgattg tttatcggct aggtcaaata gagagctttg atatctgcat gtctagcttt 360 agaattcagt ttagtgcgct gatctgagtc gagataaaat caccagtacc caaaaccagg 420 ggggctcgcc acgttggcta atcctggtac attttgtaat caatattcag tagaaaattt 480 gtgttagaag gacgagtcac catgtaccaa tagcaataac gatcggtcgg actattcatt 540 gtggtgatga cgctgggttt acatgggaaa ggtgcttgtg tcccgacagg ctaggatata 600 atgctgaggc ctttccccaa acgttcagcg tgggatttgc tacaacttct gagtcctaca 660 tgtacgtgtt catgttatgt atgcacaagg ccgagaatag gacgtagcct tcgagttagt 720 acgtagcgtg gtcgcataag cacagtagat cctccccgcg catcctattt attaagttaa 780 ttctaaagca atacgatcat atgtggatgg gcagtggccg gttgttacac gtctaccgcg 840 gtgctgaatg accgggacta aagaggcgaa gattatggtg tgtgacccgt tatgctcgag 900 ttcggtcagt gcgtcattgc aagtagtcga ttgctttctc aatctccgag cgatttagcg 960 tgacagcccc agggaaccca taaaatgtga tcgcagtcca ggaccgaagc atactgcaag 1020 acttgtaggc ccatgaatta cccgcttagg caatgggtag acctttcctt gccgcgtgga 1080 acaaaccgcc atgagtgttt tacgtttacg gtcggcgacc ggagtcacag cgcgaccaac 1140 ggggccaggg tgttaccctg atgaatattc aacggaattg ctataggcct tgaacgctac 1200 acggacgata cgaaattatg tatagaccgg gtcatcaaaa ggttatactc ttgtagttaa 1260 catgtagccc ggccctatta gtacagtagt gccttgaatg acattctctt tattaaattt 1320 tctctacagc taaacgatca agtgcatttc cacagagcgc gatggagatt cattcactcg 1380 gcagctctgt aatagggact aaaaaagtga tgataatcat gagtgccgcg ttatggtggt 1440 gtcggaacag agcggtctta cggccagtcg tataccttct cgagttccgt ccagttaagc 1500 gtgacagtcc cagtgtaccc acaaaccgtg atggctgtgc ttggagtcaa tcgcaagtag 1560 gatggtctcc agacaccggg gcaccagttt tcacgccgaa agcataaacg acgagcagat 1620 atgaaagtgt tagaactgga cgtgccgttt ctctgcgaaa aatacctcga gctgtagcgt 1680 tgttgcgctg cctagatgca gtgttgctca tatcacattt gcttcaacga ctgccgcctt 1740 cgctgtatcc ctagacactt aacaataagc gctttttgta ggcaggggca ccccctatca 1800 gtgactgcgc caaaatatct tcggatcccc ttgtccaatc aaattcatcg aattcttaca 1860 tttaagaccc taatatcaca tcattagtga ttaattgcca ctgccaaaat tctgtctaga 1920 aataggagga agccttttag ccatcgctat gccgccgctc aaacgcgccg ggtgcaaaag 1980 cccagagcaa aggcccatag gcttttccat cggcacgcga ccgattccat caagtatagc 2040 cacggtcgta acttcgacgg cggcaatagt ttatggcgct aacacctttc catcgctgag 2100 tccaaacgta tgacgtgagg tgttaggtgc aatccccatt cgggacggac acgcgagtaa 2160 aggtctaaca tgtttcaact ttccagttag tcgccgtcct atattggcaa gaccggatgg 2220 ccgtcaacca gttccattct gactatttgc cacattgatt aaagaagtag gagcgttagt 2280 cggcgcgtgc gcgggactat ttagcgaagg tcgccgtatc gcgggtggcg gcgtttccgc 2340 cataacaaca agcgtttaag gtcagagtcc gcgacgaatg aaacgccgtg caagcggctt 2400 tggtacggcc agtttagcca aagcctgatc cgttcgcctt gggttatcat gtaccagtca 2460 agcggtcatc gcgggcggcg ttatcatggt aagggcgtcg aacggtcgtg aaagtcttag 2520 taccacacgt accaacttac tgaggatatt gcttgaagct gtaccgtttt agggggggaa 2580 ggttgaagat ctcttcttct catgactgaa ctcgcgaggg tcgtgttgtc ggttccttca 2640 aaggttaaag aacaaaggct tactgtgcgc agaggaacgc ccatttagcg gctggcgttt 2700 tgaatcctcg gtcccctttg tctatccaga ttaatccaat tccctcattt aggaccttac 2760 caagtcaaca ttggtatatg aatgcgacct tgaagaggcc gcttaaaaat gacagtggtt 2820 gatgctctaa acttcatttg gttaactcgt gtatcagcgc gataggctgt tagaggttta 2880 atattgtata gcaaggtact tccggtctta atgaatggcc gggaaaggta cgcacgcggt 2940 atgggagggt gaaggggcga atagacaggc tcctctctca ctcgctagga ggcaattgta 3000 taagaatgca tactgcatcg atacataaaa cgtctccatc gcttgcccaa attgtaaagt 3060 gtctatcacc cctaggcccg tttcccgcat attaacgcct gattgtatcc gcatttgatg 3120 ctaccgtggt tgagtcagcg tcgagcacgc ggcatttatt gcatgagtag agttgactaa 3180 gagccgttag atgcctcgct gtactaatag ttgtcgacag atcgtcaaga ttagaaaacg 3240 gtagcagcat tatcggaggt tctctaacta gtatggatag ccgtgtcttc actgtgagga 3300 tcgtttgccc gtaaaactaa ctggcaaaag cctaagtgct gtttcgggtg caatggtata 3360 acttcccaca tttagccgaa tagcgccaac agcgttatta tttggtccca cccccgcagc 3420 ttgccagctc tttgagtatc atggagccta tggttgaatg agtccaataa cgaacttcga 3480 caggaaccaa gcagtgggcg atgtcagcga caattccact ttggtagagg tcgttctttt 3540 gtagaaaaca atctaacata actatgacac tttcggtaaa ttcaccacct ttaccagggt 3600 cgtaagtggt caagccacgc cgaccacttc ataagcattg gtggtttata acttgttgcg 3660 ttcctagccg ctatatttgt ctctttgccg actaatgaga acaaccacac catagcgatt 3720 taacgcagcg cctcggaata ccgtatcagc aggcgcctcg taagaccatt gcgaatacca 3780 ggtatcgtgt aagtagcgta ggcccatacg cgagataaac tgctaggaaa ccgcgtctct 3840 acgaccggtg ctcgatttaa tttcgctgac gtgatgacat tccaggcagt gcctctgctg 3900 ccgggtccct ctcgtgattg ggtagttgga catgcccttg aaagatatag caagagcctg 3960 cctgtctatt gatgtcacgg cgaaagtcgg ggagacagca gcggctgcag acattatatc 4020 ggaataacac taaggtgaga taactccgta actgactacg cctttctcta gactttactt 4080 gaccagatac agtgtctttg acacgtttat ggattacagc aatcacatcc aagactggct 4140 atgcacgaag caactcttga gtgttaaaat gttgactcct gtatttggga tgcgggtagt 4200 agatgactgc agggactccg acgttaagta cattaccctc tcataggcgg cgttctagat 4260 cacgttaccg ccatatcatc cgagcatgac atcatctccg ctgtgcccat cctagtagtc 4320 attattccta taaccctttt gagtgtccgg tggcggatat cccccacgaa tgaaaatgtt 4380 tttcgctgac aatcataatg gggcgctcct aagcttttcc acttggttgg gccagctagg 4440 cctctctgcc cggagtttcg gcgcagtgct gccgacagcc gggcattgtt tttggggcgt 4500 tattcgaggg cactcggacc taacttgtcg ggaccacccg gggtagtcat cgggcttata 4560 cagcgaaaag cccagcaccc ggctcctcgc tatggaaggt cattagctcc ggcaagcaat 4620 taagaacaac gcaaggatcg cggatataaa cagagaaacg gccgaataca cctgttcgta 4680 tcgtatcggt aaatagcctc gcggagccat gtgccatact cgtctgcgga gcactctggt 4740 aatgcatatg gtccacagga cattcgtcgc ttccggatat gcgctctatg tgacggtctt 4800 ttggcgcaca aatgctcagc accatttaaa ttagaccgac tccagatctg taaggtccgc 4860 cacgcagacg acagcccacg gagatcactg accgatctat ctgaacggcg accatttgtg 4920 tggtactggg gcggagagat aactacggtg ccgcttacaa cccctctgtc gtcgctgacg 4980 tttgtagtct agtctcatta tgattgtacg ctattcaggg attgactgat accggaagac 5040 atctcaaatg aagtggtcta tacgacagag accgtgcacc taccaaatct ccttagtgta 5100 agttcagacc aattcgtact ttgttcagaa ctcacatttt aacaacagag gacacatgct 5160 ctaccttcat gatctattga cgtccctgag gctgcaatac atgtaacgag gcagtatccg 5220 cggtaagtcc tagtgcaatg gcggtttttt accctcgtcc tggagaagag gggacgccag 5280 tgcagttatc attaatgtgg aaattgggag gactcttggc cctccgcctt taggcagtgc 5340 ttactctttc ataaaggggc tgttagttat ggcctgcgag gaaatcgcat cgacacaata 5400 aaagtacacc aaccagcaga aaacaggtac atatacagtt taacgacctg gttcttaccg 5460 gagcgcctcc gtacacagta cgatcgcacg ccccatgaga tcaatacgta taccaggtgt 5520 cctgtgagca acaaaagcct aaggatctaa gatgactaca gtagcatagc catagaacat 5580 ggcactgaaa tatcgatggg cgtaaaagtg aacaacgcag acgatggtag gaggagtcgt 5640 cagaccagat agctttgatg tcctgatcgg aaggatcgtt ggcccccgac ccttagactc 5700 tgtactcagt tctataaacg agccattgga tacgagatcc gtagattgat aagggacaca 5760 gaatatcccc ggatgcaata gacggacagc ttggtatcct aagcacagtc gcgcgtccga 5820 atctagctct actttagagg ccccggattc tgatggtcgt agaccgcaga accgattggg 5880 gggatgtaca acaatatttg ttagtcacct ttgggtcacg atctgctacc ttactggaat 5940 ttagtccgtc ctataatttc ccttgcatat aagttgcgtt acttcagcct cctaactgca 6000 cccttagcac gaagacagat ttgttcttac ccatactcca ccgttggcag ggggatcgca 6060 tgtcccacgt gaaacattgc taaaccctca ggtttctgag cgacaaaaga ggattacgac 6120 gccaagccac tataaatgct aattgagtcg aagaaggtac tcctttttgg tatttgtgct 6180 actctcctca taagcagtcc ggtgtatcga aagtacaaga ctagccttgc tagcaaccgc 6240 gggctgggag cctaaggtat cactcaagaa acaggctcgg taacatacgc tctagctatc 6300 tgactatccc ctacgtcata tagggaggaa cacagatatt gcagaagcgg gaatgaggtt 6360 gatattacat caagtatcaa cagacgacct cagaagggtg gtcttttaaa atgacaacgt 6420 acattaagat gcagacaaac actactatgc tggttaccgt gacagccgca cggatcccat 6480 ataatgcaat cgtagtctac ctgattgtac ttagaaatgt ggcttcgctt ttgcccacgc 6540 acctgatcgc tcctcgtttg cttttaagaa ccggacgaac cacagagcat tagaagaatc 6600 tctagctgct ttacaaagta ctggttcctt ttccagcggg atgttttatc taaacgagga 6660 tgtgaaaatg tcatatgtgc ggcaaaaata gacgacattt aagaacctag cattatagga 6720 tggttacatg gttttcgtta ttgctagcca gcctcataag taacaccact actgcgaccc 6780 aaatgcaccc tttccacgaa cacagggttg tccgatccta tattaggagt gttgtattaa 6840 ttcactctag agtaatggat cacggtgcca gaaactaagg tggtgtagga ctagatgcaa 6900 gtaacaacgg tcgtactaaa atcagctacg cgatcgccga cttagaaggc tgagatggtt 6960 gcataaggag accggtgtag ccaaggacga aagcgaccct aggttctaac cgtcgacttt 7020 ggcggtaagg tatcactcag gaagcagaca ctgatagaca cggtttagca gatcgtttga 7080 cgactaggtc aaattgagtg gtttaatatc ggcatgtctg gctttaaaat tcagtatagt 7140 gcaggagaaa cttgtctact ggcagacctc actatctttt aggaaggtca cgaagcggct 7200 ggagtgatgg ctagtctcag ccactgtcaa cgggaagcgc gacgtttatg gtcaatataa 7260 actagcagtt taatccgtat ggcgatctta gcggatgtta caggatggtg gaagctaata 7320 gcgccggtga gagcgtaatc tgccgtctcc accaacacaa cgctatcggg tcatattata 7380 agattccgca atgggattac ttataagtag ccttaacgat atccggaact tgcgatgtac 7440 gtgctatgct ttaatacata cctggcccag tagttttcca atatgagaac atcaattgta 7500 catcgggccg ggataatcat gtcatcacgg aagtaactgt aagacaaata attcaaaaga 7560 gatgtcgttt tgctagttca cgtaaaggtg tctcgcgcca cctctaagta agtgagccgt 7620 cgagacatta tccctgattt tttcactact attagtactc acggcgcaat accaccacaa 7680 ggagaagatg acaaagtaga agctagacaa ctatatcaaa tcgttgtgtg gtcttaaacc 7740 gacacagtag cattcatcgt tggtcgaaaa acatccgtcc ccgaggggga tagtcactga 7800 cgcggttttg tagaagccta ggggaacagg ttagtttgag tagcttaaga atgtaaattc 7860 tgggattaag gatgtggaag gtcacagcct cgttggagga gatactacgg tcccgctttt 7920 cgtgggtgac aaaggtcgca aaggtttcct tagggaggtc gtttcccgga tctttatcta 7980 catctcctgg gtgaagggga tgatctttct ctacatgtcc tgggtggagg ggagtttcct 8040 tgtatgggtg gaacccaacc cgccggtgag ggtgagttac ggtcggcggt catatgtggg 8100 tatgacgggt atgacgtcat aaatagtcgt tgtcaaaatg ggaggtggaa gtccttactg 8160 tagtcacccg tgaaggtgga gaactggcag gacatctcta ggtcacctaa acgtgcgaag 8220 tgtgcgaaat taccaccgta aattcgtcct actgaaagtg cttacgcatc ttgagagaga 8280 ttgcagagag ccaggtcaaa tacattgaga aatctgtata tgtactttgg taacgctcta 8340 aggtaggtac acgttagtga tataaaaaga aggcgacgac ggtagcagag gtctagaaac 8400 cgcatgaacg accaacttat ttgaagagct agtgcggtgg aattgggtcg gaagaactta 8460 aattgttaga gcagtttatg ggtggtaggt tacatctatt tacttcaaaa cgcggtgccc 8520 tagcccacaa gcattgcttt gtaaaattgt tgaaggtaac ttgtaaaagc cgaacatgaa 8580 cgatggtagc taagagtagg ccgggagtgt agacctttgg ggttgaataa atctattgta 8640 ctaatcggct tcaacgagcc gtacaggtgg cacttcagga ggggcccgca gggaggaagt 8700 aaactgctat tcgtcgccgt tggtggtaac taattgtgtt ccttgccact acaattgtat 8760 ctaagccgtg taatgagaac aaccacacct tagcgaattg atgcgccgct tcggaatacc 8820 gttttggcta cccgttacta agcccatcgc gattttcagg tatcgtgcat gtagggttgg 8880 accgcacgca tgttaaactg ctggcgaagt gcgattccac gaccggtgta cgatttaatt 8940 acgccgacgt gacgagtttc ctgttaatgc ttcacccgcc ggaccgccct cgtgatgggg 9000 tagctgcaca tgagcttatg acaaataacg agagtgtact tgtttaatca tctcacggtg 9060 aaagtcggga gaacagcagc cgctacacac attttaccgc aactacactt agctgagata 9120 tttccataga tgactacgca tccctctaga ccttacataa ccggatacag tgactttgac 9180 aggtttgtgg gatacagcaa tgacttgcat agctgcgtat ggaggaagga actcttgtgt 9240 gttaatatgt tgacccctgt attacggatg cgggtagaag atgtgggcag agacacccag 9300 gtcaagttct taaccttctc gtaggaggtg ttccagttca ccatacgacc atatcattcg 9360 agcatgacac tatgtacgct gtccccattc tggtagtcat catccctatc acggtttcga 9420 gtgagtggtg acggatattc cccacgaatg aatatcttat tcacagtcgg tcatattggg 9480 gtactccttg gcttttccac ttggctcggt ctgttaggcc tccgtagcgt gagtttcggc 9540 cctgtgctgc ccagtgtcgg ccattctcat tggggcctca cttctggata ccccgaccta 9600 ttttgacggc accattggcg gaagttgttg ggcttatgca ccgtaaagtc ctccgcgggc 9660 ctccccccta caaaagatga taagctccgg caagcaatat tgatcaacgc aaggatcggc 9720 gatataaaca gagaaacggc tgattactct tgttggtgtg gtatcgctaa attgcgtcgc 9780 ggagccttat ggcatagtcg tccgcggagc attctggtaa cgcttatggt ccatagcaca 9840 ttcatcgcat ccgggcatgc gctctatttg acgatccttt ggcgcagaga tgctggccac 9900 gtgctaaatt aaagcgactg cactactgta aggtccgtca cggagacgac ggcccgggga 9960 gagcactaac ccatcaacct gtacgggaac attctatatc gttctcggac ggacagatta 10020 ctagagtgcc gctttcagcc cctctgtcgt cgccgacgtc tgtaatatag cgttgttgtg 10080 attccactct attgaggcat taactgatgc ggaaggagat ctggaatgaa ctggtctatg 10140 tcacagaaac tgtgcaaata cctaatgtcg ttagtgtagg ttctgaccga tacgtgcttc 10200 gttgagaact cacaatttta caactgggga cataaaccct acgcccatca tctactgacg 10260 tccctgaggc tccagttcat gtaatgggag agtatccgcc gcaagatcta gtgcaatggt 10320 ggtatagtaa gctcgtactg tagtagaggc gacacgggta ggatcatcag taataaggat 10380 agtgggaaaa ctcacagacc accgcctata gggggtgctt acttttacaa aaagcgactg 10440 ttagtataac cccacgagga ttcgaaaagg tgaaccaacc cggtcgatcc ggagggacgg 10500 gcctcaaagc cgcgtcacga cggctgtcgg cccgtaacag aaaccccgga ataagctccc 10560 gtgagcctgg atagaacagc cctggtgggc cccatcagta gcccgaatat gtcgcttttc 10620 gggacgtggg ccgaggagcg ataccttcca ctaatcgagg ccgttcgtta atacttgttg 10680 cgttcctagc gcctatattt gtctctttgc cggcttatgt ggacaagcat agcatagcca 10740 tttatcggag cgcctcggta cacggtatga gcagacgcct cgtgagacca ttacgtatac 10800 caggtgtcct gtaagcagcg aaggcccata cgcgagatac actgccagaa aaccgcagga 10860 tagtagaccg ggaagaccaa cgttttttca ctactccgaa agaacacact gaagggaacg 10920 gtgtaggttc ggtgagtccg ggactaggtg tccatgtcgg agacctcagt ggtgacgtcc 10980 tcgtagtata ggtttaccgc caaaaaatgg gagcaagacc tcttctcccc tgcggtcacg 11040 tcaatagtaa ttacaccttt aaccctcctg agaaccggga ggcggaaatc cgtcacgaat 11100 gagaaagtat ttccccgaca atcaatacgg gaggtccacg ctctaagtcg cttgcccaat 11160 tgtggctacc gcattgctca gaacttcggg tactggccaa ccttcaagaa gcctagtctt 11220 ggtgcgtcac cccagtcacg tagcataccc gtcatcatct atggtcgtag gtccaacaat 11280 cgtggcgcca ttgggtacca cttcatcaga gctgctcgca tgaccagcta tatctggaac 11340 atcgctggtg ggtcttatgg tcgcttttgt gggaaccatc atagtcgcaa gtctggcctt 11400 cggtatggaa ctcgtcgtcc cgactacaac catagctagt ctcggtctcg gctttgttgc 11460 aatgcccttc gttactactt caacggtcgt ggtgccggtc aaaccgctcg ctaaagcaag 11520 cgaagaaaca gctaaactta gttgtgtcga aacatcctaa gtggtcgggt tgctcgtgga 11580 gcatgcagct agcccagaac ctttagcttg tgtggtcttc gtctcggtag tcagaaccag 11640 gtcggtctga atggttggac tcgccacatc aactaggtca gctattgaag tgccaactaa 11700 aggtcgtatt gaagcaatcg gtcctatggc aagtcaagtg acagtcgtag gtatggcact 11760 tgccgcgcct ccagaactcg gtcaagctac ctcataactc ggaagtcgac tgctctaaag 11820 cgaagttgtc aaacgaacag ctatgccttc gagtaaagca aggtgcgcaa tagtaacctt 11880 ggtaacttcg gaactgcgtc aagccgctaa ctccatcggt atggaagtcg ctaaagtcgg 11940 tcaaacggaa gcggctatcg tcgcaactag tcgaatagtt gtcgaagagg ccagagcaac 12000 catggttgcc attgtcttcg tggtctatgc aagacgtcct tgcgtcatgg cctaagcatc 12060 ttcttcactg gttgctatag cggaagtcgt taccgaactg gtcgccagtc gaatcgtttg 12120 ccccatggta gtcatcacca ccatcgcgga aaccaacggt ccgaactcac ggtaggtagt 12180 ccacgtatgt agcccacctt tcgaaggaat cgtggtcgcc ggtctcctag aaacggtata 12240 tgtcttgcgt cacgcttatg gaccggtcat cctggccagt agccgacttg tggtcgctat 12300 agctttcgct agtccctgcc ttaacgtgca gcatggtatg gctattgtcg tcccggctac 12360 aaccactatc gtggtcttga aactggtcgt cgctacaacc atcctagcat tggcaccagc 12420 ggtcgttgaa gccgaaggta gtctctaact ggttttcgaa gctagtcaaa cagtagcgcc 12480 atatggccta gcctgaacct tagaaaaagg agtcgaaggt cctaaacccg ctggtcgcga 12540 acgaacaaaa acggtcgtta aaggccgtcc ggaagcggaa ggtcgtcgcc ccaggtgaac 12600 aagtcctaaa gaaacaaaaa gtctctgtag tctatttaaa acccaacgca aacgcgaacg 12660 cagaatcgtc tgaacccacg tccatcatta cggattatat aaggtttctg tttcgttaac 12720 taggtagtta ttgatgtcat ccgcactaca agctaacagg aggtaacaga tagagcatga 12780 gtggtagtag ggatgaaaca ggaagtacta attacataca gggactcctg gttgggtgac 12840 cccgggacac gtacgtgtcc taataaacag gaggcgggtg gtttcctatc tactacggac 12900 agggattttc cccgagtagc gaaggatatg aaacttctag ttatagacat ataagacgta 12960 gtccaggaag tcggctgagt ccatgaggtc agttttggag gagaagtagt tgtcacgatc 13020 cttagttaag ataacaaagt ccgagtagtt atcacgactt gctgactgcg tccactatgt 13080 ttccggggtg ggcatgggga cttgcacacg gataactcca tccggattat taagacgagg 13140 tggggtgtgc tgggctccca ggaacttcat cggagtgacg tagaaccttc agtttgagtc 13200 cacgtaggac accagcgcta ttggtgccga cgacgagtac aaacatccgg aaacttagca 13260 tattaggtaa gagtttctgt atgctcggac gtcgtcgtta gagcagaccc agaaggtgtc 13320 catggacaca catcatacaa gacaacttgg tcaactttta cggggctggg gccaattatc 13380 ccgggagtcc tcacgatcag aaaccgactg gtttgtcaca ctctggttag aggaggggtc 13440 accacgatgg gtgagggggt ggtgggtgtt tatacaagag tctgggactc caaagacgat 13500 attacgacga gacgacttat ccacgtactt tgtttcccaa acccacgcaa ctccaccttt 13560 agtccacaca aactagtacg agacacccgg attcaaggtc gtgaccgacc atgtaaaaca 13620 tttgttacaa gacttctttt aaacactatc ttcctgccca gtagcggatg attatcgttg 13680 ttgctagccg gcgtggaagg taacagcacc gctgcgagcc taatgtgccg tttccacgaa 13740 cacaaggctg tccgatcgta tattaggatt ccgcaatggg gttagcaaat ggcagcctaa 13800 acgatattgg ggacttgcga tgtacatgct ttggtacaat acatacgtgt tccagttgtt 13860 atcctgtatc ggaacatcaa ttatgcatcg gaccagcata ttcatgtcat ctaggagggg 13920 cgcgtaggat aaataattca attaagatgt cgttttgcta gtatacgtct aggcgtcacc 13980 ggccatctgt gtgcagatgg ggcgacgaga cattgaccct gatttctcca cttctaatag 14040 cacacaccgg gcaatacgag ctcaagccag tctcgcagta acgctcatca gcaaacgaaa 14100 gagtttaagg ctcgctaatt cgcactgtca gggtcccttg ggtgttttgc actagcgtca 14160 ggtaggctag tatgtgtttt tccttccagg ggtatgtggc tgcgtggtca aatgtgcggc 14220 atacgtattt gctcgacgtg tttgctctca caaacttgac ctggagatca aggagatgtt 14280 tcttgtccaa ctggacagcg cttcaacgga atggatctac gttacagcct gcataaagaa 14340 aacggagttg ctgaggacga aagcgacttt aggttctgtc tgttgtcttt ggcggaaaac 14400 ttccattcag gaagcagaca ctgattgaca cggtttagca gaaggtttga ggattaggtt 14460 aaattgagtg gtttaatatc ggtatgtctg ggattaaagt atagtatagt gtgttaatcg 14520 gagacgaatt taagacacga gttcccaaaa tcaagcgggc tcattacaac ggttaatcct 14580 ggtagtttac gtgaacaatg tcctga 14606 <210> 344 <211> 12837 <212> DNA <213> Artificial Sequence <220> <223> SIRV6 <400> 344 ggcgaataga caggctcgcc tctgtcacgc taggaggcaa ttctataaga atgcatactg 60 catcgataca taaaacgtct cgatcgcttg cgcaacttgt gaagtgtcta tcatccctaa 120 gcccatttcc cgcatattaa cccctgattg tatccgcatt tgatgctacc gtggttgagt 180 tagcgtcgag cacgcgggac ttattgcatg agtagagttg actaagagcc gttagatggc 240 tcgctgaact aatagttgtc gacagatcgt caagattaga aaacggttgt agcattatcg 300 gaggttctct aactagtatc gatagccgtg tcttcactgt gctgcggcta cctatcgcct 360 gaaaaccagt tggtgttaag ggatcccctg tccaggacgc cacacgtagt gaaacataca 420 cgttcgttgg gttcacccgg gtcggatctg agtcgaccaa ggacacactc gagctccgat 480 ccctactgtc gagaaatttg tatcccgccc ccgcagcttg ccagctcttt cagtatcatg 540 gagcccatgg ttgaatgagt ccaataacga acttcgacat gataaaatcc ccccctcgcg 600 acttccagag aagaagacta ctgacttgag cgttcccagc acttcagcca aggaagttac 660 caattttttg tttccgaatg acacgtgtct ccttgcgggt agatcgccga ccgcagaact 720 tacgagccag gggaaacagt aaggcctaat taagtaaagg gagtaagtgt tcgaacgctt 780 cagatgtaac catatactta cgctggatct tctcccgcga attttaaccc tcaccaacta 840 cgagatttga ggtaaaccaa ataagcacgt agtggcgcta tccgactgtt cccaaattgt 900 aacttatcgt tccgtgaagg ccagagttac ttcccggccc tttccatgcg cgcaccatac 960 cctcctagtt ccccggttat ctttccgaag agggagtgag ggcaagacga atctgcgtca 1020 acgctaaagg ccgtcaaaaa gcaagagggc gtcgcgcctg ggaaaaaggt agtcaagaaa 1080 ccatcaagct taaatgtcgg gaaattgggt ttgcgccgcc ttcaggggca gcgatggggg 1140 cgacaattgt ggaccgtatg gactccatta tgctgggact cctcgggtgt aagggagaga 1200 ccctgcggcg tacttcattt tgtatatgca acgtgcccaa gtgacgccag gcaagtctca 1260 gctggttcct gtgttagctc gaggctagac ataggagctg aagggtagtt gttgaaaaag 1320 taggaatatc tagcctagat gtcgtttcag gcgaaggact ctaaggtctt aaaatcgttg 1380 ggtgctcatt cactaggttg cgaagcctat actgatatat gaatccaaac tagagcaggg 1440 ctcttaaaat ttggagttgt aaatacaggt caaccgctct aaagcgaagt tgttggacaa 1500 acagttatgc gtaactgtaa agcaaggtgc ccaagtagac tcttactaat ttgggaacga 1560 cggaaagtcg ctaccttcat cggtatggct ttcgctaaag tcggtcaaat ggaagaaggt 1620 agtcgaagta ctgttgaaag agtagactga gcgacagtcg aaaccagccc caatgaacaa 1680 gacgccattg caaaacgtct atacgctacg gtcaaagacg cttccacacc acatggtata 1740 gtggacgccg ttagcctcat gtgtctcact caaagacctt gcgtcacggt catcctcttt 1800 gtcagcaacg tagaaatctc cggtctgtcg ataccttgcg taaagctctc atgtagccta 1860 ccttacgaag aaatcgcaca agtcggtcgc ccagcggtgg catatgtctt gcctaacgtt 1920 tctagaccga tcagcctcac gagtagcctg cttgtggtgt ttatagttta cgctagtcca 1980 atctgtatcg tgccgcttgg tatggctatt gtcggcctgg atgcaagcac taacgtggtc 2040 atcagactgg tctgcgctat aatctacgca atagaggcac cagcggtcgt tgaagccgaa 2100 gacgctattt cggtggcttg gtcacaagtc aagccattgc ctctctgaga cgccgtatga 2160 attaatatgt aaactttgcg cgggttcact gcgatccgtt cagtctcgtc caagggcaca 2220 atcgaattcc catttgtatg ttcagctaac ttctacccat cccccgaagt ttagtaggtc 2280 gtgaggaata ttgtttcaat ggtcgtggcg ctggtcagac tgctcgctaa agtcgtcgaa 2340 gaaacagcta gaccatctat tctctgaact tcctaagtgg tcgggttggt cgtggcgctc 2400 atagttagcc cagaacgtcc agtttatgtg gtggtgatct tggttgccag actcagtatg 2460 gtcaaaatag ttgtgctcgc catttcaact agtgcagttg ctaaagtgcc aactaaggtt 2520 cgtcttgaag caatcggtag tatctgcagt caagtcgtag tcgtaggtat ggcacttgtc 2580 gtgcgtctag gacgcgttga agctatctta ttgcccgaag gtgagtgtgg ctctttgatc 2640 tgacaacgtg caacctctat cgcgaccgat tgtttctgcg gaccatgttg tcctcatagt 2700 ttgggcatgt ttccgttgta ggagtgaaac cacttagcag gagccgaaag tctgggacac 2760 gtcgtgggac gtatcgttag gactatggga ctagtcgtct tcggtcttac aaatgttgca 2820 atgccctggt acgtttgggg ttgaaaaaat ctattgtacc aatcggcttc aacgtgctcc 2880 acggctggca cctgaggagg ggcccacacc gaggaagtag actgttatac gttggcgatg 2940 gtggtagcta actaagttgc ttgccacaac aacagtatca aagccgtata aagggaacat 3000 ccacacttta gtgaatcgaa gaggcctatg cctcgtatca tctatggtcg taggtaaagc 3060 aatagcggaa acattggatg tgacttcatc aggactgctc gtatagtgag ctacttatga 3120 aacatgaatg gtcggtcttg tggtcgcttt tgtcgaaatc accggttatt gtatgagtag 3180 tgatttgtaa gagttgttag ttagctcgtt caggtaatag ttgcccacac aacgtcaaaa 3240 taagagaacg gtcgtaacat tatccgtgat tttcttacta ctatcagtac tcacgactcg 3300 actctgccgc agctacgtat cgcctgaaag ccagtcagcg ttaaggagtg ctctgagcag 3360 gacaactcgc gtagtgagag ttacatgttc gttgggttct tccgacacgg acctgagttg 3420 gccaacgtcc cacttgaggt ctgtgccccg gtgatgagaa gtatgcatct cgttcttgca 3480 gcttgtcagt actttcagaa tcatggcgtg catggtagaa tgactcttat aacggacttc 3540 gacatggcaa taaccccccg tttctacttc tagaggagaa aagtattgac atgagcgctc 3600 ccggcacaag ggccaaagaa gtctccaatt tcttatttcc gaatgacatg cgtctccttg 3660 cgggtaaatc accgaccgca attcatagaa gcctggggga acagataggt ctaattagct 3720 taagagagta aatcctggga tcattcagta gtaaccataa acttacgctg gggcttcttc 3780 ggcggatttt tacagttacc aaccaggaga tttgaagtaa atcaattgag gatttagccg 3840 cgctatccga taatctccaa attaaaacat accgttccat gaaggctaga attacttacc 3900 ggccttttcc atgcctgcgc tatacccccc cactttcccg cttatctgtc cgagcggaga 3960 cagtgcgatc ctccgttaag atattcttac gtatgacgta gctatgtatt ttgcagagct 4020 agcgaacgcg ttgaacactt cacagatagt agggattcgg gtaaagggcg tataattggg 4080 gactaacata ggcgtaaact acgatggcac caactcaatc gcagctcgag cgccctgaat 4140 aacgtactca tctcaactca ttctcggcaa tctaccgagc gacttgatta tcaacagctg 4200 tctagcagtt ctaatctttt gccaacatcg taatagcctc caagagattg atgatagcta 4260 tcggcacaga actgagacga cgccgatgga tagcggactt ttggtcaacc acaattcccc 4320 acgggacagg tcctgcggtg tgcatcactt tgaatgtaca agcaacccaa gtgggccgag 4380 cctggactca gctggttcct gtgtgagctc gagacttggg atgacagctc tttaaacata 4440 gagcgggggc gtcgaacggt cgagaaagtc atagtacctc gggtaccaac ttactcaggt 4500 tattgcttga agctgtacta ttttaggggg ggagcgctga aggtctcttc ttctcatgac 4560 tgaactcgca agggtcgtga agtcggttcc ttcaatggtt aaaaaacaaa ggcttactgt 4620 gcacagagga acgcccatct agcggctggc gtcttgaatg ctcggtcccc tttgtcattc 4680 cggattaatc catttccctc attcacgagc ttgcgaagtc tacattggta tatgaatgcg 4740 acctagaaga gggcgcttaa aattggcagt ggttgatgct ctaaactcca tttggtttat 4800 tcgtgcatca ccgcgatagg ctgacaaagg tttaacattg aatagcaagg cacttccggt 4860 ctcaatgaac ggccgggaaa ggtacgcgcg tggtatggga ggatcaaggg gccaatagaa 4920 aggcttctct ctcactcgct aggaggcaaa tgtaaaacaa tggttactac atcgatacat 4980 aaaacatgtc catcggttgc ccaaagtgtt aagtgtctat cacccctagg gccgtttccc 5040 gcatataaac gccaggttgt atccgcattt gatgctacca tggatgagtc tgcgtcgagc 5100 gcgccgcatt tattgcatga gtagggttga ctaagaaccg ttagatgcgt cgctgtacta 5160 atagttgtca acagaccgtc gagattagaa aatggtacca gcattttcgg aggttctcta 5220 actagtatgg attacggtgt tttcactgtg ctgcggctac ccatcgcctg aaatccagct 5280 ggtgtcaagc cattccctct ccgggacgcc gcatgtagta aaacatatac gttgcacggg 5340 ttcactgcgg tccgttctga gtcgaccaag gacacaatcg agctccgatc tgtatcctcg 5400 acaaacttgt acccaacccc cggagcttgc cagctcctcg ggtatcatgg agcctgtggt 5460 tcatcgcgtc cgatatcaaa cttcgtcatg ataaagtccc cccctcggga gtaccagaga 5520 agatgactac tgagttgtgc gatccctgca cttcagctaa ggaagctacc aatatttagt 5580 ttctgagtct cacgtctgac ctcgcgggta gattgccatg cgtagagctt acgagccagc 5640 ggaaagtgtg aggcgctttt aagtatggcg agtaagtgat ccaacgcttc ggatatgact 5700 atatacttag gtttgatctc gtcccgagaa ttttaagcct caacatctat gagttatgag 5760 gttagccaaa aaagcacgtg gtggcgccca ccgactgttc ccaaactgta gctctttgtt 5820 ctgtcaaggc ccgactttca tcgcggccca ttccatgtgc ggaccatacc gtcctaattc 5880 tttggttatg tttccgatgt aggagtgaac ctacttgcct ttgcgtctta ataccaatga 5940 aaaacctatg cactttgtac agggtaccat cgggtttctg aactctcaga tagtggggat 6000 cccgggtaaa gacctatatc tgcggtccaa cttaggcata aacctccatg ctacctagtc 6060 agacccaccc tgcacgaggt aaatatggca cgcgtccgac ctggttcctg gcgttctacg 6120 ctgccacgtg ttcattaact gttgtttggt agcacaaaag tattaccatg gtcctagaaa 6180 ttcggcacag ttagttcgag cctaatgtca caaatgacgc agaacgccaa tgagtgttag 6240 acattaggtg gagttcagtt cggtaacgga gagactctgc ggcatactta attatacatt 6300 tgaaacgcgc ccaagtgacg ctaggcaagt cagagcaggt tcccgtgtta gcttaagggt 6360 aaacatacaa gtcgattgaa gatgggtagg gggcttcaaa tcgtccagca ctctacagta 6420 cctccgagag caagtagggc accctgtagt tcgaagcgga actatttcga ggggcgagcc 6480 cacatcgtct cttctgcgga tgacttaaca cgttagggag gtggagttga ttccatcgat 6540 ggttataaat caaaaaatcg gaacgctgtc tggaggatga atctaacggt gcgtatctcg 6600 atcactcagt cgcttttcgt actgcgcgaa agttcgcacc gctcatacac ttggttccga 6660 agcctgtcct gatatatgaa tccaaactag agcggggctc ttgacgtttg gagttgtaaa 6720 tatctaatat tccaatcggc ttttacgtgc accaccgcag gcggctgacg agggactcac 6780 accgagaaac tagacagttg cacgttggaa gtagcgccgg ctaagaaaga cgcctggtac 6840 agcaggacta tgaaacccgt acaaaggcaa catcctcact tcggtgaatc gaaacgcggc 6900 atcaaggtta ctttttggat acctgaaaca aaacccatcg tagtccttag acttgggaca 6960 ctttcacctc tagggcccat atctggaaat agacgccaag ttcaatccgt actccgacgt 7020 acgatggaac agtgtggatg tgacgagctt catttatacc cttcgcgcgc cggaccgggg 7080 tccgcaaggc gcggcggtgc acaggaaaca ttaaatcaaa gaagtttatc aaccaattag 7140 tacagtggca tgttaagtaa aacagaaaga acgccaagtg gcaacaaatg acgtaattat 7200 ttaagcaaaa agcgtaaacg cttacaaagg ctatttaaat atcagtaaat cgttggaaat 7260 tgggtatgtg ctgccttcat acgcaaaaat ggatgcgaca gaaacgagtt gtatggcctc 7320 cactacgctc gcctgccgcg gaccctaaac gacagtcgtt tgcccaacta cggctacggc 7380 atcgctcaga acttcgggtc ttggcccacc tttctgacgc ctagtcaacg tgcgtcgcct 7440 cagtcttggt ctgtatgacg gtatctgctg atcggtaggg agaccgagaa tctatcgggc 7500 tatgtcacta aaactttcca aacaccccat gtcgatactg aacgaatcga tgcacactcc 7560 cttccttgaa aacgcataaa catacaagtg ggcagatgat gcgtacgccc ttctaataca 7620 tccaacactc tacgccctct tcaagagctg gaagggcacc ctgcacttgg ataagagatt 7680 atctcgtaag gcaagctcgt accgtcattc atgcggaaga gttaacacga ttggaagtag 7740 ggatagtttc gaacctcggt tactagtcct aataagggaa cgctgtctga aggatgagtg 7800 tcaaggacaa aaagaagaaa tatcaattgc aaaaataact ataggtactt ctttgaagaa 7860 accaaacgtg agtttctaac acgactaaat ccatttgatc tttgagaaag tcgaagacga 7920 atggtggcat gtgccatgct agcctgcgga gcactctagt tatgcatatg gtccacagga 7980 cactcgtcgc tttcggattt gccctctatg tgacggtttt taggcacact tatgctcagc 8040 accgtttaaa ccagaccgac actagatcta taaggtccgc catgcagacg agagcacacg 8100 gagatcaccg agcaatctat ctgatcggcg accatttgtg tggtactgga gccgagaggt 8160 aactacgatg ccgctaacaa cctctcggtc gtcgctgacg tttatagtct agtctcatta 8220 taatcgtacg ctattcaggg attgactaat accggaaaac atctcagttg aagtaatgta 8280 tacgacagag accgtgcacc taccaaacct ctttagtcta agttcagact aattggtagt 8340 ttgtctagaa ctcagatttt atcactagag gacgcacgct ctatttttat gatccattga 8400 tgtccctgag gctgcaatat atgcaaccag gcagtcttcg cggtaagtcc tagtgcaatg 8460 gggctttttt tccttagtcc tcgagaagag gagacgtcag tccagatatc tttaatgtgg 8520 taattggaag gactcttggc cctccaccct taggcagtgt atactcttcc ataaacgggc 8580 tattagttat gaggtccgaa gattgaaaaa ggtgagcgaa ctcggccgaa ccggaaagac 8640 ggacttcaaa gcaacctgac cacggttgcg cgtccgtatc aagatcctct taataagccc 8700 ccgttactgt tggttgtaga gcccagaacg ggttggccag atgtgcgact atatcgctta 8760 gtggctcttg ggccacggtg cgttaccttg caggaattga gaccgtccgt taatttccct 8820 tgcatatata ttgcgttttt ttgtcctttt atccgctcac ttagaaaaga gacagatagc 8880 ttcttaccgg tgcgcctccg tacgcagtac gatcgcacgc cccatgagaa cgataggtaa 8940 acctagtgtc ctgtgagcga caaaagctta aatgggaaat acgcggccat aagttggagg 9000 tgggtttata tcgcgtatcc atccaactta actgaaacgc gaagcagtag acagtgttga 9060 tcgagatttg cgaagggaaa cagtaaaagt ccagggtgtc gttaattacg acaaaactaa 9120 agactgtata gttagaagga tcaattgggc tgatttagaa atggtataag ttaaagaaat 9180 ggaaacttat tcgacaaaaa gaaaaactca tgatgtatag atggaaactg ccataatgtc 9240 gattcgagtt atatttttag attttggtaa aatatccaac atgtattaac gaaattttga 9300 agtatagatc aaactattct tcaggtacag catcaagcat tcggtagcat aagccaaaca 9360 agtctttgaa gggttggaca tagcgagttg tgtagaaagt tacgaagact tcaacagaaa 9420 aagagtttaa aaggaccacg tatgccaaat cgacaacaat atagtcgttc tattctgcga 9480 tatgtaagat agagacacat cggcaggtag catagaactt gggtcaatta ttatttaagt 9540 gcggtaacaa acaaacttag ggaatattat tgtgctacag caaatgcctt gaaaagtcga 9600 gaaagactac tatactcgtc aagttcgtaa agacttaaca gtaattgtaa tgcaaatact 9660 tagcgagatt gattccttcc cagtagtcat ctttccttta gatattagaa cagcaatttt 9720 agattttgta catagtggag aaaaggacgt cattttaatg gatgaaccag acgttaacgt 9780 catttacttg gtcgttgatt tcgaagactc agtcatcaaa attgtagctt cgctagctaa 9840 tgtgcttaaa atcatcatag tcaaagtgta cgtggtcgtg gtaataaaca accttcatta 9900 ctgtacaaaa gtgccagttt gttacattat cgaacgctgt atagaggtat catttcacct 9960 atttgacaac taggtgataa accttcggtc gctagacgtt gttgttattg aaaaaatgga 10020 agataatcta tacttaatcc aaaacaaccc agaccaagct cgtatataaa tggaagtcgt 10080 caaaattggt ttcgttcaaa taagttggga ctgatgaatc agttttctga acttcaccgc 10140 tattagcgtc ctagttgtct acataattag tatcattatt gtagtagttt aaatcgctaa 10200 caaccaaaaa atccttgaca tgggtaacga cgttattggg gaaccaatgc tcagtgttgg 10260 tgaaagaatc gttaggtcga actattggta cgttctaata aaagacgaca tagaaattcg 10320 ttacataatc aacgtcacta ctaagggcgt attgcaaatt agttcataaa ttattaacag 10380 tttgcaccta atagttctag tccttaaaaa ggtagaagac gtaggtaatg tcagttgcct 10440 caatgtagtc aggtaaaaag tatatgtgct gatggcaaac ttcatctttg tgagagttga 10500 gcatgtataa caacatcact cagtataacc gcttggttac acaattagta tcatgcctat 10560 agaacgcctt atcattcagg tcgtcgtgat agtgaagggg atcaaagtca aaatctactt 10620 tgtcataact ctttacggta tcccaatgct tggtataagt gcttaagaaa ttgtttaaac 10680 agaaactcaa gtataagcga aaacaagtta cctggaaaaa gaagaagtgc gtataattga 10740 tgaacgagtc gaagtcgaag ttgttcaaca agagagttag gtagtgccgc ttgaaaaagt 10800 aatagtgcaa ataagaaagt aagactgtat cggagacgac tttttaatag tagtaattct 10860 cggtacaact aaaccagtaa tttcagacac tgaaatagag gacttcgtta ggtacgaagc 10920 aagttaacta aatgacaatt aaattgaggg taaatatcac aaagaaatca tggaagattt 10980 cgaccgaata caggaaaatt tagattaaag gtttgtcgtc gttgaaataa gaagaagcga 11040 aacggcattc gtttaacaaa acgtcacata tttaggtata gtagattgcg ttcacaaaga 11100 aatttaaagt cttgtaatta tggtggtcga tttattggta gtatatttgg aaatagcatg 11160 caattttgga atggacgaag aacctgacat ctgagctatt gtaagtttaa tttaccattt 11220 cgtcattgta tatgcggtaa acgtaaattt acacatatga cacgaacttt gagttttatt 11280 agcctgccta attgttggta acaatgtaat ggaaattggc gatgactatg gggaaagagc 11340 aatcaactca ccataaattt gtgaacgcag ttaagtaatt ggcatcgcgc gcctaatcgt 11400 ttacattcgc atggaacaag aagaaagaga cctattcgtg ggagtcaatg gctatgtgga 11460 aaaacatgaa ctgccaaatt cggctaacca accagaaatg gtcatggaaa attcttgcgt 11520 caaagttcgc ggtgacgttt acgaagaagc taaacttgtg cttgtttaga aagttaggtg 11580 cgtcctggtt ttagtaaatt tagaaaacca ttttgttgtt tgcgacagtt aaataaaact 11640 taacgacaaa gaagtgactt gcgtcgtaga ttaactggaa attaaagtgg tatctaaaat 11700 ggggtttgcc gatgcggtct tcaaagtcta aaattcttga agtttgcgtt tggtcgcaaa 11760 aattatggat ttagtcgatt attgcctact aagcagcagt ttagaagttt ttaagatagt 11820 tacgctagaa gaccgggagt atacttagtt caatcttcaa taccttatat tattgtacac 11880 ctaccggtca ccagccaaca atgtgcggac ggcgttgcaa ctttcagggc ctaatctgac 11940 cgttctagat acggcactgt gggcaatacg aggtaatggc agacacccag tgtcgaacaa 12000 cacctgacct aacggtaaga gagtcacata atgcctctgg ccgcgtgccc agggtatatt 12060 tggtcagtat caaatggact gagatgaatc tttacaccga agcggaaacg ggtgcgtgga 12120 ctagccagga gcaaacgaaa aatcctggcc tgcttgatgt ctcgtaatgt tcttagagat 12180 ggacgaaatg tttcacgacc taggaaaagg tcgccctaca aaatagattt gtgttactct 12240 cttcataagg agtccggtgt accgaaagaa caaggcgagc ctaggtagca accgccggct 12300 acgggggtaa ggtatcactc aagaagcaga ctcagtaaca cacggtctag ctgactgtct 12360 atcgcctagg tcatataggg acctttgata tctgcatgtc cagctttaga attcacttta 12420 gcgcgcagat ttgggtcgag ataaaatcac cagtacccaa gaccaggggg gctcggcgcg 12480 ttggctaatc ctggtacatc ttgttatgaa tattcagtag aaaatttgtg ttagaaggac 12540 gagtcaccat gtaccaaaag cgatattaat cggtgggagt attcattgtg gtgaagacgc 12600 tgggtttacg tgggaaaggt gcttgtgtcc caacaggcta ggatataatg ctgaaaccgt 12660 tccccaagcg ttcagggtgg gatttgctac aacttccgag tccaatgtgt ccgtgttcat 12720 gatatatatg ctcaagggcg agaattggac ctagctttcg tgttagtacg tagcatggtg 12780 acacaagcac agtagatcct gcccgcgtat cctatatatt aagttaattc ttatgga 12837 <210> 345 <211> 148957 <212> DNA <213> Artificial Sequence <220> <223> SIRV7 <400> 345 agttatctca tcttaatatt attgtgatat aatatttgca atcgctgctg tctctccaac 60 tttcgccgta agatcattag acaggcaagc tcttgttata ttttacaagg gcatgtctaa 120 ctatccaatc ataagaggga ttcgacagca gaggtactgc ctgaaatatc atcatgttag 180 cgaaattaaa ttatgtacca gtcgtaaaaa cacggttttt tagcagttta ttttgcgtat 240 aggcttacgt tacttacacg atacctggta ttcgtaatga tcttatcaag cgtctgttga 300 aacagtattc cgaggcgctg cgttaaattg ctatgatata gttattttta ttagtcgata 360 aaaagacaat tatagcgatt aagaatgcaa ctaattataa cgaacggcct caaatagtag 420 aaaatatccc tcctccgggc atctcctaaa atgccatata ttcggattat tgaagatggt 480 taccacggca gttttatcta gtctcatagg tctttatttc gaggttattc ttaccgactg 540 tgatgcgtcg tgtcccagct ttgaatgcga tatcaccgga ttgtctgact tggttcacct 600 tttagattcc tcattgggtt atactaactg acacttattc tttaagtaag gactccttat 660 tggcagtggt gaatgagatt tggcattatc cttattattg ataattttac tcctgtcgca 720 tgaattacag tacgagctta ttttaccagg ataccacttg atcttatgga ggatactctt 780 tcaattcttg aattggaccc acagagatga gtgtactaga tgaacgtaca cattatgtgc 840 tcagttgtat aattgtgtgt tttcaagaaa gaaggtatac gtcaatacat tcagtatcga 900 cataaggtat ttggatagtt tcagtgatat agaccaatag atttcagatc tctttacgta 960 tcagtaaata cctttataga gttgattcat attaacacta attacgcttg gaagaatagg 1020 ttttatacgc tctatttcat ctattttggc tataacagtt gtcggaatta aatagttctc 1080 tcccggaact atagttacaa ttatcttaac cttaatttcc gccactgtta ttgtctatac 1140 aagtttatgt cttactaaga taacttgatc caccataaca cgttgcatga acctctccct 1200 ttgcaagttg tctgctataa aaatgcgctg actttctgcc agtggattct aaatctttat 1260 tgtggcgacc gccaagtgaa atatacagtg taaaaccata aagctgaata taactaccac 1320 ttccacttct gccaccaagt agaccatgct aagttaccac cctattttga atgtcactat 1380 caccgtactt accatattgt tagttaagga taccacccca gcaacgtgat tgcagtctat 1440 tattagccca acaagacctc agaatgcgaa gtagcttata gttttcgttt gtccgtggcc 1500 acataaatat aggtttgtgt ctgtttcacg gacctaattt ggctaaacgt aagtgcgaca 1560 gattacgtct attacgaata agctcactgc caatataata caaaccaaga ctactcttga 1620 tactaatgcc acgcccatag tccaaaagat ttctttcttt atttccagaa caagtttaac 1680 aattacctgc tatacgttgt ccacctctat gttgttagct tcgtcccata ccgtttaaat 1740 tatacgactt tgctgcacta ccattatcca tataagtata tgtctcatgt ctggatgaca 1800 gacatccaag tctactacgt cctctatcct atcgaagatt gagttaaata tctgcatgaa 1860 taagccggcg tcgattaaac gtataatgaa gacgaccgtg ttaacccgca agctgtagtc 1920 gcgcatttat gttcaataga tagcttttag ttatattgct atctctactt gttgaccttg 1980 taagttttcg ataagaattg aatggataat cttgcaccaa actatttcga ctcagacttt 2040 aaaatcgatc tctcgactct cttctatctt ttaatagcct tctgtggata tttgaactat 2100 ctatgcttcc aaactaacga cttctccacc tcttaaatcc taattttctc aaacagtgca 2160 tactactgtt tcctctttaa cttccatatc gcatactagc agatacctaa gtagaatagg 2220 gacaatagtt tcttgttgtt gattcttagt tctttaacct cctcagtttc ttacgttacc 2280 gtcttcccaa acgtcgatta tctgttacct atcccggtct tcttcgactt ctcgataatc 2340 taaaactata tcgttatgtt tacttacttc ttcccggtga tttaggtcct catttgggta 2400 aatcccatgg accttattgt cttcgtcttt tcgttcttat atcattgtat aatgttgggt 2460 tcaatgttct agatttcccc ttttaagttc tccattttga ccttcttcct ttacgtccat 2520 tcaaatcttc tcgttctaaa aattctatga gactactttg tcataacagg gattaagtga 2580 acaagtatcc tataacaggt gtaaatacgt ctgctgtact taatcctaga aattctgtac 2640 tgtatctata tcttcgtgaa gttcttctcg caatacttgt gtcaccaatt aacagttaac 2700 gaatacatga cgatgcggaa cacctcgggc caggccgcgc acttcctata tttactttga 2760 gcagtgtagg tgtaataata tagcatgtag cctcatgctt aacttgaacc ggagataaaa 2820 atctatgcac gcgaccttca catttacaag gtttagattt ttaatgctac ccaaaacaaa 2880 gtccataggt ttttcattaa gactacggcg caaagttaag tgatttcact cctacaacag 2940 aaatatgccc aaactatgaa aacaacatgg ttcgtagaaa taatcaatcg taccattgca 3000 atgtctacct tgttgttagt ctatactact attgcctcac cgagttcgaa acttacaccc 3060 cggtgcacca taatctaatt tacgactatt ttaactataa ttgccattat ccttctgata 3120 ttataatgaa aacacaatgg tttattgtca attacttatg cactgttaca tcttgtggtc 3180 ctcggtccag cctgcgtagg atataaatga agcacgtctc atccagattg atctatcgta 3240 cctccaaata cgaattcaac ttggcgtcta tatccagaaa tgagccctag aagtgataga 3300 cttccaaatc tttaaactac catgggatat gtttcacgta tccaaaaagt aattataatt 3360 ctgcgtttcc attcacttaa gtgaggatgt agcctttata ttggtttcat agttttgtta 3420 taccaagcat gtattttacc tagcactatt ttcaacctaa aactgttttg tttctcgatg 3480 tcaaaccctt gtcagctacc tgcggtggtg cacgaaaaaa ccgattagaa tattaaatat 3540 ttacaactcc aaattttaag agccctgctc tagtttaaat tcatatatca gtataggctt 3600 cgcaatttag tgaataagcg gtatgaattt tcgcggagta tgaaaagtga ctaagtaatc 3660 gagatacata ccgttagata gatgcttcag actgcattct gaatttttga tttataacca 3720 tcgaaggaat caatttcatg ttcctaacgt gttaagtcat cagtaaaaaa gaccataagg 3780 gctccccttt tgaaataata ctgtttcacc accattcctt tagtttaatt cttgatgatt 3840 tcttctcaaa ttacgttggt tagcatgtga aagtttatat aatttgctct aacatcctgg 3900 gttaatatgc agaaaatggg cttagggatg atagacactt tacaaattgc acaagcaatc 3960 gaaatgtttt atgtatcgat atagtatgca tttttataga attgccttct agcatgacag 4020 aaacaagcct atctattcgt ttctttatcc tcctatacca cgtgtgtacc tttcccggcc 4080 attcattaag accaccacaa atatgtctgt tgttccagtt tctttcgtgt ctacgtgtct 4140 ccttttgtaa ctgagcgata ccaagagttt aatatgttcc tttgatgtcc ttattctatg 4200 tataaagagt aataatctta agtctggagt ttactcagca accagttgag tctaggcaca 4260 gctttcttgg gttgcttata tattgaaaat aatgtactca acaggatcag tctcataagg 4320 aattgtgatt gaccaaaagt ccgctatgca ttgacgtcgt ctcagtttag tacctataac 4380 tatcatcatt cttttggtgg ctattacaat gttggtaaaa gaataaaact acaacacatc 4440 tgttaataat caactcgctc gattgattgt caagaatgtt tagtgatgag tatgttattt 4500 aaggcactcg agttgtgatt gagttggatc cattgtagtt tacggctata taagtcctca 4560 ataatacgct ctatacgcaa attcctatta tctgtgaaat gtttaacgcg tacgctaact 4620 ctataaaata cttaactata ttatacgtta gaatatctta acggacaatc gcattgtatt 4680 tatttgtata gattaatgaa aaagtaggtg cgaatattgt aggaccagcg atatgttcta 4740 cgagttcgat ttgaacttct ccgttttgtt ttgcgtcttg atttccgatc tttgcgactt 4800 ttctttcgtc ctgacctgct ttgagctttc ctttatacta actggtattc tggcgaaaca 4860 aagctgtagc agaatcatgt catgagtatt aatagtagta agaaaatcac ggataatgtt 4920 acaaccgttt tcttatttag aggtagtgtg aagaactttt acatgaacca gcaaactaac 4980 atctctttca aattactact catacaataa gttccgtgac gacatgacta tcaccaagta 5040 tgtaactacg agttctacta gaatttctct tccttagcgt tcgcatacga ctaccatttt 5100 aaagccttct tctcgtcctg gggaacctct gcctacaaaa agcgtttatt gttaattgtt 5160 taaagataca gtgaaaacga cagtcgttaa aacaaaaatg atatgtacaa tactacatct 5220 aagcagcgca ataacgcaac agcttgaagt atttttcaca gaaaaatcgt acgtccagtt 5280 ctaacacttt catatgtgtt cgtcgtttat gcttttagta tgaaaactag tgtgccggtg 5340 tctggagatc atccttcttg catatgactc caaggacagc catcatggtt tgtaagttca 5400 ttgagactgt tacgcttaac tggacgaaac tcgagaagat atatgattga tcgtaagacc 5460 gctttgattc gacaccatca taacacagta ctcatgatta tcatcatttt tttagtccct 5520 attacaaagc tgccaagtga ataaatctct atcacactct atgaaaatgc acttgatcgt 5580 tttattgtag aaaaaattta ataaacagaa tgttaatgaa ggcactactg tactaataga 5640 gttgggctat atgttaacta caagactata acttattaat aactcggtct atacataatt 5700 tggtatcatg tatgtagtgt tcaagggcta tagcaattct gataaaaatt tattaaggta 5760 acaccttaga ataatatact ggtctatcgc aatacaacca cttctaccgt ataatccgag 5820 agtggccacg ataatcgaag gtatggtcaa ccgttgtaag taattctagg cgataaggaa 5880 tccttttttt ataaattttc tttaggacat tgttcacgta aattatcaag attaattgtt 5940 gtaatgagct cgtttgattt tgaaaatttg tgtcttaaat tcgtctccga ttaatacact 6000 atactatact ttaatcccag atatatcaat attaaactat ttaatttaat ctaatcctca 6060 aaccttctat taaatcgtgt caatcaatat ctgcttccta aatggaagtt tttcgccaaa 6120 aacaataaac agaatttaaa gtcgcttttg tcctcagcaa ttttgttttt tttatacagg 6180 ttataacgta gattcattcc gttaaagcgt tgtccaattg gataaaaaac atctttttaa 6240 tctccaggtc aagtttgtga aagtaaacac gtcgagcaaa tacgtatacc gcacatttga 6300 ccacgcagcc acatacctct ggaaggaaaa acacatacta acctacctga cgctagtgca 6360 aaacattcaa gaaactctga caatgcgaat taacgagcct taaactcttt tatttgctga 6420 taagcattat tgcgaaacta gcttgagctt gtattgtctg gtgtgtacta ttagaagtga 6480 aaaaatcagg gttaatgtct cgtcgcctca tttatataca gatagtcagt gatacctaaa 6540 cgtatactag caaaatgaca tcttaattaa attatttatc ttacatattc ttcctagatg 6600 acatgaatat gttggtccga tgtataattg atgttctgat ataggataat aactgaaaca 6660 catatgtatt ataccaaagt atgtacatca caagttccca atatcgttta gactactatt 6720 tactaatccc attgtggaat cttaatatat gatcggatag ccttgtgttc gtgaaaacgg 6780 catattaggc tcacagcggt gctattacct tccataccag ctagcaacaa cgataatcat 6840 ccgctattgg ataggaaaat agtatttaaa agaaatcttg taacaaatgt tttatatagt 6900 cctaattaac tacattactc gagcgaacta aaacttatga tcacaaaaat aaagctgagc 6960 ctaattatgt gatatgattt aaaatttcgg tctatatagt tataatttga tgaaataaat 7020 tggattagca gtttgctaga tgatttggca cagatagtca tagacgaagg acttactatg 7080 aaatagcggt tttagttgtc aatgttggat ttcagcgaaa gcaggaatcg atgtagctaa 7140 agaaatatgt ccatcattgc atttagataa ggcatcttgc caaaaagaga accaatattt 7200 tgtaaagaaa ctagaggtcc tctttatact ctatgatttg tgcagctctt aaatgcaaac 7260 gacgagtaaa caggtgcgtg ggtgtatgga gaccttcatt tttatgtatg attggatgga 7320 ctacgatcac attttatggg ttctctgcga ctgtcacgct aaattactcg gaaattgaca 7380 atgtaatcga ctacttgtaa taacccactg atcgaactgg agcataacgg gtcacacatc 7440 ataattttca ctattttagt ccgaattatt cagcatcgca gtaaatgtat aacaatcggc 7500 cactgtccat ttacatatca tcgtattcca ttagaattaa tttaatatat agaatgtgta 7560 agtaggatct actgtactta tatgaccatg ctacatatta acatgaaaac tatatccaat 7620 tcttgacctt gtgtatatat aatatgaata cgaacatgtt ggattcaaaa gttatagcaa 7680 atcccatctt aaatgtttag ggaaaggctt tagtattata ttctagccta ttggaacaca 7740 agcacctttc ccatgtaaac ccagtgtctt caccataatg aatactttca tcgatcgtta 7800 ttgcttttag tacatgatga ctcgtccttt taacataaat tttctactga atattcatta 7860 caaaatgtac caggattaac caatgtaatg agcccccttg attttgggta ctggtgattt 7920 tatttcgact cagatttaca cactaaagta aattttaaag ctagacatac aaatattaaa 7980 gctctctatt taacctaaac gataaacaat cagctaaacc gtgtcttact gagtctgctt 8040 cttgaatgat actttaccgc caaagtcaac agttactacc taaagtcgct ttgatctttt 8100 gctacaccga attctttatg aagagagtaa cgtaaatcta ttttgtagaa cgattttttt 8160 ctaggtcatg aaacatttcg ttcatctcta agaaaattat gagacattaa acaggtcaag 8220 aattttcgtt tgctcctcat tagtccacgc acccattttc gtttcgatgt aaaaattcat 8280 attagtctat ctgatgctaa tctaatatac cctagacacg ctgacagagc cattatgtga 8340 gccttttact gttaggttag gtgttgttcg atactgggtg actagcatta cctcgtattg 8400 cccagagtgt ggtatttaaa atggtcaaat taggctctaa aagttgtaac gccatctgca 8460 cattgttggc tggtgaccgg tagatgtata ataatataag gtattcaaaa ttgaattaaa 8520 tatgctttgc gctcatccta catgagataa atatagtgga acgatgcata attctacatt 8580 cgattcaggt tatgaacagg aactcgtatt taaagttttt atcgttcaaa aacctaagtc 8640 ttcaataacg ttttgggtgg gatttgcaaa ccccttacca aaatcgtaat ataggattgg 8700 ataaacttgt cttcgtggta agggtacaat tgggacgctg aagtagtgta acttatgaga 8760 aaggctaatt ataacaaaaa ttaaattcta gtgaggtgga agattgtatt ttaaagatga 8820 ctaataaata ttgttataca tgctcctaat cggttctacg gctcgcgggc accaaaaccc 8880 atgacctcta aaataaagct gagtttagac gcgtgatttc atttaaaatt tcaatctgtt 8940 tgtctattat atccagagat atactgcatc tcctattagt ttatcgattt cgcatacaat 9000 gactcagaca aagaactcac tatggaatcc gagaatcagg tgccaatgat cgttccgatc 9060 aaaacatgaa agctatgtgg cctgacgaat acttctctca ttgtttttag ataaaagatc 9120 ttgctgaaat aggatccagt actttgtaaa gcaagttgag aatcttataa tgttttgaag 9180 atcatcgggt tctaaaaacc aaacgtggac taattaggtg cgtgggtaaa aatctagcta 9240 catttctaag tagtttcaga taaattatga taagtttata tgggatgcgt acgccagtct 9300 gcataatact ttgagagaat gacaatccaa tctagatcaa gctatgaatt acaaacggtt 9360 atgaagtaaa acgggtgtta cagcttaaat ttagccagaa taatttagga gtttcaacat 9420 tgccgtagag gtgtaacaac tcaccagtgg ccatccacat attaatatat tccataactt 9480 ttaactaatt ttatttgtaa cgcgggcaaa atatattttg tttaagtctg cttgctacga 9540 tcttagacat aagcttggtt caatactcgc gcttaaagaa aaatttcaaa aacagcaagt 9600 ttttggactc tcaaattgtt acaaaatgca ccctgtacgt taggggaatg gttactactt 9660 tatatccatt cttgtttaga cagaagcacg attcccatgt taatcctcca acttcattac 9720 attgaatata cgttcccttt aattttctat ttgatttaag gtcattccat catctaacac 9780 taaatttcta ctgattattt ataacaatat gtaggaggat taggcaagat gcgaaatgct 9840 ggttgttttt ggttctggac attttatctc gaattaaatc tgcgcattaa tatgaattct 9900 atagattgac aagcagaaaa cataggtctc tttaagacaa tgggaataat taaataccta 9960 gagcataaat tactgagtaa atatcttgac tcatatcata gattcctagc ccccggttgc 10020 taggaaggtt agtcttgtag tttccataga ccagactgct aaagaagaga gttactgata 10080 tctttttttt agggcgacgt aatcctagat gatgattcat atggaccaac acttagaata 10140 ttacaaatct tctagttacc taatattttt gatttgcacc tgggaaatca ttgcattcga 10200 ttttagatcg gtgtaaagat tgatcaaact ccatttaatt ttatccaaat ttaccatatg 10260 catgggataa cacgtattat gaaattcact tttcgttagg ttacaactag ttcgagactt 10320 attgtttgct aatactacat tatcctcaca ttagcgaatg taattggctc tctaaaaatc 10380 ctcatagttg taatgggaag ccgagattat tgagtgatca ccagtagctg tactatttta 10440 taagctatta aaaattgatt aaaataaaca taccgcctgt aatatataaa tcatagtcag 10500 acgaacattg ctacaatctg tattcgtacc aagttatgag cgcgtatttc ttatttaagt 10560 ttttgtcgct cacaaaacac tgggtttacc aatcattcac atgggacatg cgatcgtact 10620 gcctatgatg atatattggt aagaagttat ctgtctttct tctaagagag caattaaaag 10680 gttaaagaaa tgcaatatat atgcaaggga aattaatgga taatcttaat tcctgtaagg 10740 tagcacattg tgacttaaaa atgactaaat aatattgttg tacatctagc taatccgttc 10800 tgggctttac gaccaacagt aacgaagacc tattaaaaag accttgatat ggacgcgcaa 10860 ctatagttaa gttacctaga tgtccgtctt ttctattcag ccaaattctc ttaccttttt 10920 caatctttgg atctcataat taatagctcg tttatagaag agtatatact atctaagggt 10980 ggagggccaa ggatccttcc aatcacaaca tcaaagatat ctggactgac gttttctcct 11040 ctcaaggact atagaaaaaa aatcccattg cattaggatc taccacaaag attacctggt 11100 tgtaaatttt gcaatgttaa gaacatcaat ggatcataaa aataaaacgt gcatccttta 11160 gtaacaaaat ctaagatcta gacaaatttc caactagtct gaacttagat taaagaggtt 11220 taataggtac acgaactctg tcgtatatat tactttaagt gaaatgtttt ccagtgttga 11280 tcaatctctg aataacaaac gattataatg aaattagagt gtaaacgtta acaacgaccg 11340 agagattttt agcagcatca tagttacctc tcggctctag tatctcacaa atggtcatcg 11400 atttgataaa ttgctcgata atttctatgt actttcattt gtatggcgga ctttataaat 11460 ttagtatcag tctggtttaa acgatgttga gcataagtgt gcttgaaaac tgccacataa 11520 agggtaaatt cgaaaacaac gagtgttttg tgaaccatat gcataattag agtaccctgt 11580 acgctagcat gacacatgcc tccacaaagc tattcttcaa tacagtataa atagattcat 11640 tcgtctattt ttctatttat atgcgttata tttacgttgt ctttaattac ctgctagaat 11700 taaagacatt ccattgtgta ggactgagtt ttcagtaatt cattatatta acttgaagac 11760 caattaggca agacccgaca agttggttgt gaatgcctcc gaataattct tctaaaacta 11820 tgcctgggtg ttgacagcaa tccattgaaa cttcaggcag aaaagcttag ccggtttaag 11880 tgaatggaaa aacttagaag cgttctgtat tgattatcga ggaaatattt tctcatttgt 11940 gacagatttc cacctcccgg ttcttaggaa ggttaaaggt gtaattacta ttgacgtgac 12000 tgcagaggag aagagatctt gttttcattt tttaacggta acgtgatcct gaatggcgtt 12060 tataatggag caatatattt aacgtcggaa ttcttgtagt tatctagtat ttttattttg 12120 tatataggag ataataattt tacattcaag atttgtttca tgcttaatca gtcttgaatg 12180 taatttctct aaatcatcca tgtgccagag acagcatatt taatgaaata aactttacaa 12240 aaggtcataa ttagttaggg agttatcgta tgttaatatt actctgatct gatatttgca 12300 atcgctgctg tctctccaat attcgccgta atatcaatag agaggcgaga tcatactgtg 12360 ttttccaacg gcaagtctat ctaaccagtt attagaggaa cttgatagca gacatacacc 12420 atttctattt caatgctatt tgcttatagc tttgcctaac cttcttgttt ttgttatatg 12480 actactggtc aattcactaa acaggttatt aggactctaa tttcgttcat aacttgttcg 12540 tttagttctt cgcgttcttc gaaatcctat caatttcatg ttcctaacgt gttaagtcat 12600 ccgcaaaaaa gacgataaga gcttgccttt cgaaataatt ctgtttcaaa ctataggctg 12660 ccctacttgg tcttcaagat actatagagt cttcgatgat ttaaagcccc caacccatgt 12720 tcaatcaact tgtatgttta tccttaaact aacataggaa ccagctaaga tttgtttagt 12780 atcatttggg cacgtttcat atatataata aagtatgccg caaagtttct ccgttaccga 12840 actgtactcg acctaaagtc taatactcat tggtgttcta tcacatttgt gacattaggt 12900 ataaattaac tctggagaat ttctacgact ataataaaag ttttgtgcta ccaaataact 12960 gttaataaac atgtcactat gtagaacgcc aagaaccagc tgggatgaat gctttattta 13020 cttcgtgcaa agtaggtcta agtagatagc atcgaggttt atgcttaagt tgaaccgcaa 13080 atataggtct tttgtcggga tctccactat ctgtaaattg tgaaacctgt tggtacccta 13140 tacaaagtgc atagctatat cattaataat aagacgtaaa cgaaagttta ttcactccta 13200 cttcggaaag ataaccaaag aattaggaga atatggtgcg cacatagaaa ggatcgggaa 13260 ataactttga ccttcacaga acaataagtt acaatttggg aacagtcgga gaacgccact 13320 acgtgctttt ttggcttacc ttatatttta taattgatga ggattaaaat tcacgggaga 13380 agatctaact taagtatata gttatatctg aagcgtttga tcacttactc cctttactta 13440 aataggcctt attgttttcc ctgactcata agttctatgt ttggcaatct atctacaagg 13500 tcagacgtat cacttaaaaa ctaaaaattg gtaacttcct tagttaaagt gctaggaatg 13560 ctcaattcag tagtcttttt ctctggaaat cgcgaggggg gaactttatc atgtcaaagt 13620 ttgttattgg actcattcaa ccataggctc tatgatattt aaaaagctag taaactacgg 13680 gggtaggata caaatttctt gataatagaa atcggaattc gaatgtgtcc ttggtcgact 13740 cagatctaat ccaagtgaac ccgacgaatg tatatatttt attacatatg gcgtcttaaa 13800 cagggaatcg cttaacatca actggttttc agataatgag taaccgcaac atagtgaaaa 13860 cacagttatc catactagtt agagaacctt taataatgct gctaccattt tctaatcttg 13920 atgatttgtt gacaattatt agtacagcga ggcatctaac ggttcttagt caactctact 13980 tatgcaataa atgttgcatg tttgatgctg actcaaccat ggtagcatca aatacggata 14040 caattaggcg ttaatatgca gaaaacgggc ctaggggtga tagacacttt acaatttgga 14100 caagcaatgg aaatgtttta tgtatcgata tagtatgcat ttttatacaa ttgctttcta 14160 gcaagtgaga aagaagccta tctattcgtt tctttatcct cctataccac gtgtgtatct 14220 ttcccggcca ttcattaaga ccggaagtat cttgttatac aatattaaac ctttaacagc 14280 ctatcgcgct gatacacgaa ttaaccaaat gaaatttaaa atattaacta ctgttatttt 14340 taagtggcct cttcaagatt gcattcatat atcaatattg acttggtaag gttctaaatg 14400 agggaattga attaatctgg ataaacaaaa gggactgagg attcaaaata ccaaccgtta 14460 aatagatgtt cctctgtgca tagtaaattt ttgtttttta acctttgaag gaatcgacaa 14520 cacgaccctt gcgagtttag tcatgagaag aagagatctt caaccttccc cccttaaaat 14580 agtacagctt caaacaatat cctcagtaag ttggtatgtg tggtactaag attttcacga 14640 ccatttgatg cccttattct atgtataaag agttattatc ttaagtctga agtttactca 14700 gcaaccagtt gagtctaggc ttagctttct tgggttgctt atatattgaa aataatgtac 14760 acaacagaat cagtctcata aggaattgta attgaccaaa agtctgctat acattgacgt 14820 tgtctcagtt tagtatctat aactatcatc aatctcttgg taactattac aatgttggta 14880 aaagattaaa actacaatac atctgttaat aatcaactcg ctcgatagat tgtcaagaat 14940 gtatagagat gagtatgtta tttaaggcgt tcgagctgtg attgagttgg atccattgta 15000 gtttatgcct atataagtcc ccaataatac gttttttacc cgaatcccta ttatctgtga 15060 aatgtttaac gcgtacgcta gctctataaa atacttagct atattatacg taagaatatc 15120 ttaacggaca atcgcattgt atttgtttgc atagataaat gaaaaaatag gtgcgaataa 15180 tgcaggcatg gtaaaggccg gtaagtaatt ctagccttca tggaacgtta tgttttaatt 15240 tgaaaattat gggatagcgc gtctaaatcg ttaattaaat tagttgaaat cacctagtta 15300 gtatctgtaa aaattcacca aaaaagttcc atcgtaaatt tataattact actgaatgat 15360 tccaaaattt actctcttaa gttaattaga ccaatctgtt tccctaggct tctataaaat 15420 accgttggta attttcccgc atggagacag atgttattcg gaaataaaaa tttagaaact 15480 tctttagcac ttctgctgac aacagttatg tttatactat tcttctcttg atgtagaaac 15540 gaggcgttat tatcatgtcg aagtttatta taagtgtcat tttaccatac agaccatgat 15600 tctgaaagta ctgacaagct gcaaatatga aattcatatt tctcatcact agggtacaga 15660 cctctaataa gatgtaggtt aactgagatt cgtgtcggaa gaacccaacg aacatataac 15720 tttcactata tgagttgacc tgctctgagc tttccttaat actaactggt attctggcga 15780 aacatagctg cagcagaatc aagtcatgag tattaatagt agtaagaaaa tcacggataa 15840 tgttacaacc attttcttat tttgaggtag tgtgaagaac ttttacttga accagcaaac 15900 taacaactct ttcaaatcac tactcataca ataaattccg tgagcacatg actatctcaa 15960 cctggataac aattaatgct catatattga gaagttatta agcgagatat gcgtttaagg 16020 ataatagaca ctttacaaat tgcggatacc attgagatat tttataaata gatgcaatat 16080 ggaatcttat agaattgcct gttagcgtta tatagatgaa catgactaat taccctttca 16140 tccacgctat tagcttccat accatttgcg gccattcatt aagatccgtt gttccttaca 16200 ataaaaaaat taataaaaaa ttccctaaca tgcagattta gtagttattt taaacaactt 16260 tagtggaaca aactaaaaca tttttaaaca gaattttgat agtggcaatt aattactaat 16320 gatgtgatat taaggtctta aatgtaagaa ttcgataaat ttgattagac aaagggatcc 16380 gaagatattt tagcgcagtc actaataggg ggtacctctg cctacaaaaa gcgtttatta 16440 ttaaatgttt aaagatacag tgaagacgac agtcgttaaa acaaatatga tatgaacaat 16500 actacatcta agcagcgcaa taatactaca gcttgaagta tttttcacag aaaaatggta 16560 cgtctagttc taacactttc atatctgttc gtcgtttatg cttttagtat aaaaactagt 16620 gttccggtgt ctggagatca tcctacttgc aattgactcc aagcacagcc atcatggttt 16680 gtaagtacat tgagactgtt acgcttaact ggacgaaact cgagaagata tatgattgac 16740 cgtaagaccg ctttgtttcg acaccatcat aatacagtac tcataattat catcattttt 16800 ttagtcccta ttacaaagct gccaaatgaa taaatctcca tcacactcta tgaaaatgca 16860 cttgatcgtt taattgtaga gaaaatttaa taaagagaat gttattcaag gcactactgt 16920 actaatagag ttgggctata tgttaactac aagaatataa ctttttaata attcggtcta 16980 tacataattt cgtatcatct gtgtagtgtt caaggcctat agcaattctg ttaaatattt 17040 atcaaggtaa caccttagaa tattatattg gactatcgca atacaaccac ttctaccgat 17100 taatgcgaga gtggccgcga taatcgaagg tatggttaac cgtagtaagt aattctaggc 17160 aataaggaat gctttttttt taaatattct ttaggacatt gtacacctaa attatcaaga 17220 ataattgttg taatgacctc gtttgatttt gaaaatttgt gtcttaaaac cgtctccgat 17280 aattatacta tactacactt taatcccaga tatatcatta ttaaactatt taatttaatc 17340 tattcctcaa accttctatt aaatcgtgtc aatcaatatc cgccttctaa acggaagttt 17400 ttcgctaata acaataaaca gaattcaaag tcgcttttgt cgtcagcaat tttgttttta 17460 ttatacaggt tataatgtag attcgttccg ttaaagcgtt gtccaattcg ataaaaaaca 17520 tctttttaat ttccaggtca agtttgtgaa agtaaacacg tcgagcaaat acgtatacca 17580 cacatttgat cacgcagcca catacctctg gaaggaagaa cgcatactaa cgtacctgac 17640 gctagtgcaa aacattcaag taactctgac aatgcgaatt gacgagcttt aagctctttt 17700 atttgctgac aagcattgtt gcgaaactag cttgtgctag tattgtctcg tgtgtactat 17760 tagaagtgaa aaaatcaggg ataatgtttc gtcggttcac ttatatacag atagtgagtg 17820 atacttaaac gtatactagc aaaatgacat cttaattaaa ttatttatct tacatatact 17880 tcctagatga catgaatatg ttggtccgat gtacaattga tgttctgata taggataata 17940 actgaatcac atatgtatta taccaaagta tatacatcac aagttcccga tatcgtttag 18000 actactattt actaatccca ttgtggaatc ttattatatg atcggatagc cttgtgttcg 18060 tgaagacggc atattaggct cacaccggtg ctattacctt ccataccagc tggcaacaac 18120 gattatcatc cgctattgga taggaaaaaa gtatttaaaa gaaatcttgt aacaaatgta 18180 ttatatagtc ctaattaact acattactcg agcgaactaa aacttttgaa cacaaaaatt 18240 aagcagaggc taattatgtg atatgattta aaatttcggt ctatatagtt ataatttgat 18300 gaattaaatt ggattagcag tttgctagat aatttggcac agttagttat agacgaagga 18360 tttactatga aaaagcggtt ttagttatca atcttagatt tcagcgaaag caggaatcga 18420 tgtaactaaa gaaatatgtc catcattgca tctaagtaag gcattttgcc aaaaagtgaa 18480 cctatatttt gtaaagaaac tagaggtcct ctttatactc tatgatttgt gcagctcttt 18540 tatgcatatg acgagtaaac aggtgcgtgg gtgtatggag accttccttt ttatgtatga 18600 ttggatggac tgcgatcaca ttttataggt tctctgggac tgtcacgcta aattgctcgg 18660 aaattgagaa agtaatcgac tatttgtaat aacgcattga tcgaactcga gcataacggg 18720 tcacacatca taatcttcac ttttttagtc ccaattattc agcaccgcag taaatgtata 18780 acaatcggcc actgtccatt tacatatgat cgtattgctt tagaattaat ttaataaata 18840 gaatgtgtaa gaaggatcta ctgtacttat acgaccacgc tacatattaa cttgaagact 18900 atatcctatt cttgaccttg tgtatacata atatgaatac gtacatgtag gattcaaaag 18960 ttatagcaaa tctcatgtta aatgtttagg gaaaggcctt agtattatat tctagcctat 19020 cggaacacaa gcacctttcc catgtaaacc cagtgtcatc accataatga atagtttgat 19080 cgatcgttat tgctattagt acatgatgac tcgtcctttt aacataaatt ttctactgaa 19140 tattgattac aaaatgtatc aggattaact aatgtaatga gcccccttga ttttgggtac 19200 tggtgatttt atttcgactc agattaacac actaaactaa attttaaagc tagatatata 19260 aatattaaag ctctctattt aacctaatcg ataaacaatc aactaaaccg tgtcttactg 19320 tgtctgcttc ttgaatgata ctttatcgcc aaagtcaaca gttactacct aaagtcgctt 19380 tgatcctttg ctacaccgaa ttctttatgg agagagtaac gtaaatctat tttgtagaac 19440 gatttttctc ttggtcatga aacatttcat tgatctctaa gagaaatatg agacactaaa 19500 caggtcaaga attttcgttt gctcctcatt agtccacgca cccattttcg tttggaagta 19560 aaaattcata ttagtctacc tgatgctaat gtaaaatacc ctagagacgc tgacagtgcc 19620 attatgtgag ccttttactg ttaggttagg tgttgatcaa tattgggtga ctagctttac 19680 ctcgtattgc ccagagtgta gtatttaaaa tgattaaatt aggcttaaaa agttgtaacg 19740 ccatctgcac attgttggct ggtgactggt agatgtataa taatataagg tattcaaaat 19800 tgaattatat atcttttgcg ttcatcctag atgacatgaa tatagtggaa cgatgtataa 19860 ttctacattc gatttaggtt atgaacagga actcgtattt aaagttttta tccttgtaaa 19920 acctaagtct tcaatatcgt ttagggtggg atttgcaaac cccttaccaa aatcataata 19980 taggatcgga taatcttgtc ttcgtggtaa gggtacattt gggacgctga agtagtgtaa 20040 cttatgaaag aagctaatta taacaaaaat tatatactag tgagctggaa gattgtattt 20100 aaaagatgac taataaatat tgttatacat gctcctaatt ggttccatga ctcgcgggca 20160 ccaaaaccca tgacctctaa aataaagctg agtttaaacg cgtgatttca tttaaaattt 20220 caatctgtat gtctattata tccagagata tactgcattt cctattagtt tatcgatttc 20280 gcatacaatg actcagacga agaactcact atggaatcgg ggaatcagat gccaatgatc 20340 gttttgatca aaacaagaaa gctatgtggc ttaacgaata cttctctcat tgtatttaga 20400 taaaagatct tgctgaaata agatccagta ctttgtaaag caagtagaga ttcttataat 20460 gttctgtagt tcatcgggtt ctaaaaagca aacgaggact aattaggtgc gtgggtaaaa 20520 acctagctac atttctaagt aaaatcagat aaactatgat aagtttatat gggatccgta 20580 cgccagtctg cataatactc tgagagaatg acaatccaat ccagaacaag ctatgagtta 20640 cagacggtta tggagtataa cgggtgtcac agcttaaatt tagccagtat aatccaggag 20700 tttcaacatt gccgtagagg tgtaacaacc caccactggc catccacata ttattatatt 20760 ccataacttt taacttaatt tatatgtaac gcgggcaaaa tgtattttat ttaagtcagc 20820 ttgctacgtt cttagatgta agcttggttc aatactcgcg cttgaggata aatttcaaaa 20880 atagcaagtt tttggactct caaattgtta caaaatgcac cctgtacgtt tggggaatgg 20940 ttacaacttt atatccttac ttgtttagac agaagcacga ttcccatgtt aatcctccga 21000 cttcattaca ttgaatatac gttccgttta atattgtttt taatttaagg tcattccatc 21060 ttctaacact aaatttctac tgattattta taacaatatg taggaggatt agccaagatg 21120 ccaaatgctc gtagttttag gttctggaga ttttatctcg attcaaatct gcgcactaat 21180 atgaattcta tagtttgaca agcagataac ataggtctct ataagacata gagaataatt 21240 aaatacctag agcatatgtt actgagtaaa tatcttgact catatcttag attcctagcc 21300 cacggttact aggaaggcta gtcttgtact ttcgatacac cagactgcta atgaagagag 21360 ttacagaaat ctttttttta gggcgacgtt atcctaggtc atgattcata tcgtccaaca 21420 cttagaatat tacaaatctt ctagttacct aaaatttttg atttgcacct gggtaatctt 21480 tgcattcgat tttagatcgg tgtaaagatt gatcaaactc tatttaatat tatccaaata 21540 taccatacgc atgggataac acgtattatg aaattctctt ttcgttaggt tacaactagt 21600 tcgaaactta ttgtttgcta atactacatt atcctcacaa taccgaatat aattggctct 21660 ttaaaaatcc tcatagttgt aacgggaagc cgagattatt gagtgatcac cagtagctgt 21720 actattatat aagctattaa aaattgatta aaataaacat accgcccgta ttatataaat 21780 catactcaga cgaacattgc tacaatctgt attcgtacca agttatgagc gcgtatttct 21840 tattaaagtt tttgtcgctc acaaaccaca gggtttatca atcatttacg tgggacatgc 21900 gatcgtattg ccaatgatga aatataggta agaagttatc tgtctttctt ctaagagagc 21960 aattaaaagg ttgaagaaat gcaatatata tgcaagggaa attaatggat aatcttaatt 22020 cctgtaaggt agtagattgt gatttaaaga tgactaaata atattgttgt acatctacct 22080 aatccgttct gggctttacg accaacagta accaagacct ataaaaaaga gcttgatatg 22140 gacgcgcaat tatacttaag atacctagat gttcgtcttt tctattcagc caaattctgt 22200 tacccttttc aatctatgga tctcataatt aataactcat ttatagaaga gtatatacta 22260 tctaaggatg gggggccaac gatccttcca atcagaacat caaagatatc tggactgacg 22320 ttttctcctc tcaaagacta tagaaaaaaa atcccgctgc attaggatct accactaagt 22380 ttacctggtt gtaaatcttg caatgttaag aagatcaatg gatcataaaa ataaaacgtg 22440 gaccctttag taacgaaatc taagatctag acatatttct aactagtctg aacttagatt 22500 aaacaggttt aataggtaca cgaactctgt cgtatataat actttaagtg aaatgttttc 22560 cagtgttgat caatctctga ataacaaacg attataatga aattggagtg taatcgctaa 22620 caacgaccga gagattttta gcagtatcat agttacccct cggctctaat aactcactaa 22680 tggtcatcga tttgataaat tgttcgataa tttctatcta gtttcatttg tatggcggac 22740 tttataaatt tagtatcagt ctggttataa cgatgttgag cataagcgtg gttgaaaact 22800 ggcgcataaa gggtaaattc gaaaacaacg agtgttttgt gaaccatatg gataattaga 22860 gtaccctgta cgctagcatg acacatgcct ccataaagcc attcttcaat acagtaaaaa 22920 tagattcatt cgtctatttt tctatttttt tacgttatat ttacgttccc tttaattacc 22980 tgctagaatt aaggacattc cattgtgtaa gactgagttt ttagtaattt attatattaa 23040 cttgaagacc aattaggcaa gacccgaaaa gttggttgtc aatgcctctg aataattttt 23100 ctaaaactat gcctgggcgt tgatagcaat ccattgaaac ttcaggcaga aaagcttagt 23160 cggtttaagt gaatggaaaa acttagaagc gttcagtatt gattatcgag caaatatctt 23220 ctcatatgtg atagattccc acctcccggt tcttaggaag gttaaagatg taatttctat 23280 agacgtgact gcaaaagaga agagttcctg ttttcatttt ttaacggtaa cgtgatccta 23340 aatggcgttt ttaatggagc aacatatata acgtcggaat tcttgtagtt atctagtatt 23400 tttattttgt acataggaga tgataatttt acattcaaga tttgtttcat ggttaatcag 23460 acttgaatgt aatttctcta aatcatccat gtgctagaga cagcatattt aatgaaatag 23520 actttacaaa aggtcataat tagttaggga cttatcgtat gttaatatta ctctgatctg 23580 atatttgcaa tcgttgctgt ctctctaata ttcgtcgtag tatcaatgga gaggcgagat 23640 catagtgtgt ttaccagcgg caagtctatc taaccagtta ttagaggtac ttgatagcag 23700 acataccgcc tgaaatattt agatcatagt cagattaaat ttactacaac tcgtaatcac 23760 acgaattttt ggcagtgtat ttcgcatata agcttttgtt gctcacagga cacttggtat 23820 acgtaataat ctcatgagac atctgatcat actgtgtacc gaggtgtttc gataaatagc 23880 tatgttatat ttatctatat aagccgataa aaagacaaat ataggcatta aaaatgcaac 23940 aaaaattaat gaacggtctc aattactgaa aggtatcact ccttgaccca aaactcaaaa 24000 agtgatatat tcgaactact gatgaaaccc atcagggctg ttcaatcaag acttacggga 24060 gcttattatg aggattttgt tacggaccga caaccgtcgt cacgtaactt tgaagtccgt 24120 cttttcggat cgaccgaatt ggttcacctt tttgaatctt cgcaagataa tactaatagc 24180 tgctttatat aagagtaaac actgtctata ggtggaggcc tatgagtctt tccaatatcc 24240 ttattaatga taattccact catgtcacct ctactatagt acaaatgtat tatactgcca 24300 ttgcactaga acttacggca aatactgtct cattacataa attgcagcct caaggacatc 24360 aatagatgat aaatataaag cttatgtcct caattgtaaa attgtaagtt cttaacaaag 24420 tacgtattag tcagaactta cattaatgag attaagtact tacacagttt ctgtcatata 24480 aattagttca tttcaaatct ttttccgtat tagtcaatgc ctcaatagga tagaattata 24540 ataagactat attataaacg ttagcgacga cagagaggtt gtaagcggca ttatagttat 24600 ctctccgttc gagaacgata taaaaagttc ccgtacagat tgataggtta gtattctccc 24660 tgagctatcg tctgcatgac ggactttata gtagtacaat cactttaatt taattcatgg 24720 tcagcatttc tgtgctaaaa aatcgtcaaa taaagcgtat attcgaatac aatgaatgtg 24780 ctatgaacca taagcattac tagagtactc cgcagacaac tatgtcataa ggctccgcga 24840 cgcaatttaa cgatactata tcaataaaaa taatcagcta tttttctgtt tatatcgcta 24900 atttttacgt tgtttaatat tgcttgccag agtttatcat cttttatagg gaggaggccg 24960 gtagaggatt ttacggtata taagcctaat gactactacg agtagtcccg tcaaaataga 25020 tcagagtatc cacaaataaa actccaataa caatggctga ctcttggcag cacagcatcg 25080 aaacttgagg cacaaaggcc taacagactg aaccaagtgg aaaaactaag aagcatccta 25140 atatgattaa ctgtgaataa gattttcatt tgtgagggat atccatcacc agttactcaa 25200 aatcgtaata ggaataataa ctattaaaat gagaacagtg tacataatgt catgcttgaa 25260 taatatggtg gtatggtgat ctagaatacc tcttatgaga gagttatgta tttaacctgg 25320 gtgtctctgt tcatatctac tatttgtatc tctaatacag gagttaacat attaacactc 25380 aagaattgtt tcttccatat acagttatgt aagtgattac tgtaatccat aaacctgtca 25440 aagtcactat atctggttaa gtaaagtcta gagaaatgca taatcaatta cggagttatc 25500 tcaacttagt ataattgtga tataatgttt gcagtggctg ctgtctctcc aactttcgcc 25560 gtgagatgat taaacaagta gactcttgtt atatattaca aggtcatgtc taactaccct 25620 atcacaagag cgatccgaca ggtgaggtat tagcaggaat atcatcatgt cagcgtaatt 25680 aaattatgca ccagtcgtaa aatcacggtt tattagcagt ttaacatgcg tatagtctaa 25740 ccctacatac acgatacctg aaaatcgtaa tgatcttagt aagggattgt taaaacggta 25800 ttccgaggcg gtgcattaat ttgctatggt atggttattc ttattacacg gtttagaaac 25860 aattatagtg gctaagaatg caactagtta ttacgaacgg ccacgaatag tagaaaattt 25920 ccttcctgcg ggcttctcct gaaatgccat ttatacgggt tattgaagcc ggttactacg 25980 acagatttat tcaacctcaa aggtctatat tcccagctta ttcttagccg atatgttgcg 26040 tcgtgtccca gctttgaatg cgttaacacc ggattgtctg acttagttca gcttttggat 26100 acctcatcgg gttatactaa ctgacactta ttctttaaat aaggatccct tattgggagt 26160 ggtaagtgag atttgggatt atccttattt ctaatgatct tacccctttc gcatgaataa 26220 cagtacgagc ttattttacg aggatatcac ttgatcaagt ggaggatact cttccaattc 26280 ttgaatagga cctagagagt tgaatgtatt agatggacgt acgcattatg tgctcagttg 26340 tataattgtg tgttttcaag gaaggaagta tgcgtcaatt cgttcagtat cgacataagg 26400 tatttggaaa gttttagtga catagaccaa tagattttag atctctttac ccatcagtaa 26460 ataccattat agagtcaatt caaatttacg ctatttaaca cacaacgccg aggataaaag 26520 ggctgaaatt gacagtctac tattttgttc atgtgtgaac aattaacaat attcgaatac 26580 acgttgatgc gatagtgctc tggctaggct gtccacttcg tatattttct ttgagtagtg 26640 cagacgtatt aatataatat gtagccagga tcttagcatg aaacgatgat taaaatgtac 26700 gcataccacg ttgggatgta tgtgctatag acttttagta ttacctgaaa catagtccat 26760 cgattttgcc ataagacttc ggcacgaaat taagtgattt cacacctaca agagaaatgt 26820 gccgaatcta tgataatatc accgttcgtt gtattaatca ataatggtag cggctgcata 26880 ttatcaaatg aaggagcgac acccggggag gagtttacgg tggatatccc gtacaacttc 26940 ggctaatcat gttatctaaa taagttgggg tttgcagtta tgagggcgaa atcagattcg 27000 actatacagc gcagttcaga gccctaactc acgtaatcgt gaccttacag agcgagtcaa 27060 gttgataacc tatcgagtag ctcaatatga gtgagtgtga ataagaaatt tattgcaagg 27120 caataatcct aatcattgac agcaaacttt gataagaata aagattagta caattcagaa 27180 agcgtactta ctgtcatact cgaatgaaat gctttattaa agaaaaagtt gacgtcctat 27240 gagaagatta agaacttctc ctgcatctca caacttacat aatcttcggg catgggtaat 27300 agataggtga acatataaat acagaaaagt ttctaccttc atacgtagtt aagcaagtca 27360 tagctatatc ccataaacct ttcataatca ctgtatcggg ttatctaaga actaatggta 27420 tggctagtca tttatggtaa tttgtcagtt aagtcaagat gcggtaaatt gtgtattgcg 27480 gctcctatta tcccgactta aactgtctga tgttataaca aatacacagt agttaattgt 27540 tatttgctta tgtactgcta cgctttgtgg agaccggtcc ggcgcgtgaa gcatataaat 27600 gaaactcatc acatccacat aattatatcg tacatcggtg taggaatcga acttggcttc 27660 taattttaca tgcgtgcgct gcaagtgtaa atataccaaa tctaaaaatc acgatgggct 27720 ttgtttcagg tatctaaaag gaaattctga tgccgcgctt caattcacta aagtgtggat 27780 gttctcttta tacgggtttg atacttttat tgtagcaagc atctttatta gttaatacta 27840 tccccaacgt ataatagttt acttcctcgg tgtgggcccc tcgtcaagtg cctgctatag 27900 agcatgttga agccgattag tataatagat tttttcaact ccaaacgtca acagtcccgc 27960 tttagtttaa gttcatatat cgggacaagt ctcggaatta agtgcataag cgctgggatc 28020 tttcgcgcag ttcgaataat gagtaactga tcgagttaca caccgtcaga cttattcttc 28080 agataacgtt ccgttattac gattaataac tatcgtttga aactattcca tcttcttaac 28140 gtgttaagtc tttcgcaaaa aagacggtat gagcttacct tacgaaataa tttcttttcg 28200 aactacaggg tgctctactt gctcttgaag aaatcgtaga gtgttggatg aattaaaagc 28260 ccctacccat tttctatcga cttgtatgtt tatccttatt ctaacatgga agtttgcatt 28320 gatttgttta gtatcaatta ggcgtgtttc aaatgtatta ataagaatgc cgtatagatt 28380 ctccattacc gaactgaaca ctacttagta ttaaacagtc attgctgttc tacgccattt 28440 aagacattac gctcaaaata actgagctga atttcagaga ctataatatt atttatgtgt 28500 caccaattaa cagttaacga atacgtgacg atgtggaaca ccaggagcca ggtcggacgc 28560 atcctatatt tactttgtgc agagtaggtc taacaaaata gcatgtaggt ttatgcttaa 28620 gttgaaccgc agataaaggt ctatactcgg gatcttcact atctgaaggt ttagattttt 28680 aatggtaccc taaacaaagt gcataggttt ttcattaaaa ttatggcgca aagctaagtg 28740 aattcactcc tacaacagaa atatgaccaa agtatcaaaa caacatggtt cgtagaaaaa 28800 atcaatcgtg ataaaggttg gattttgaca aaacaaagag ccacactctg ggagcagtcg 28860 acggacgtca ccacgtgcat tttcggctaa tcttataatc tataaatgtt gaggtttaca 28920 gttctcgggg cgagatcaaa tttaagtata tagtcgtatc cgaagcgtta aatcacttat 28980 tcgccatgct tgaaagcgcc tcatgctttt cactgattca ttagctctat gtatggcaat 29040 ctaagtacga agtctgtcgt aaggcttaaa aactaaatat tggtagctac cttagttaag 29100 gtacaaggat tgcacaattc agtaggcgtt ttttctgcta ttctcgaacg gaaagcttta 29160 ttaagacaaa gcttgatatc ccacgggatg aaccagaagt tctatgatat ctcagaagct 29220 actaaatttc gggggttggg tacaagttag ttgaacatac aaataggaat ttgattgtat 29280 ccttagtcga ttctaaacaa atcatagtaa acccgtgcaa agtatacata ttaattcata 29340 cggcgtttca aagaggcaat ggcttgactt gagctgaatt acagattatg agtaaccaca 29400 agatagagta aacactgtaa tccatgttta attgagacca cttaaagatg ctgatattat 29460 tttcaaaaca caatggttta ttgacaatta tttatacact gatacatctt gcggttcttg 29520 gtcgacccta cttacgatat aaatgaagca cgtttcatcc agattcatct atcgtagctc 29580 caaatacgaa ttcaacttgg cgtttatatc cagaaaagag ccctagaggt gatagacttt 29640 taagtctttg gactaccatg ggatatgttt cacgtatcga aatagtaatt attattctgc 29700 gtttgctttc atataagtga ggatgaagcc tttatattgg tttcttaatc ttcttatacc 29760 acgcgtgtat ctttcctagc cctttattga atctggaagt gtcttgttac tcaatgtcaa 29820 acccttgtca gcctcttgcg gtgatgcacg aaaaaaccga atggaatata aaatattaac 29880 tactcctaat tttaagtgcc ctcttctaga ttgaattcat atatcaatat agacttcgca 29940 aactagtgaa tgaggggtat gaatttatcc ggaataacaa aagggactaa gtattcgaga 30000 tacaaaccgt tagatagatg ctccagtctg catagtgaat ttttgatttt taaccattga 30060 aggaatcaat ttcacgatcc ttacgagtta agtcatcaga aaaagagacc attagcgctc 30120 cccctttgaa atagtacagt ttcaaacaat agcctgagta agttggtatt cgagatacta 30180 taaacttttc gatcatttga tgcccccaac ctatgtttaa agaactatta tctttagcct 30240 taaactaaca caggaaccag ctaagtcttg tttagcttca cttgggctgg ttacatatat 30300 aaaataatgt atgccgcaga atttgtccct tagcgaattg tagttgacca aaagtctatt 30360 actcattggc gttgtatcac ttttgtgtca ataggtatga ttaatctctt gaaaattttt 30420 acgacaatgg taaaagatta gaactactaa acaactgtta ataatcatgt cgctccgtag 30480 attgccaaga atcagttggg atgaatacgt tatttacaac gtacaaacta cgactgagtt 30540 ggtaccatcg tagtttatgc ctatgttgaa ccgcaattat acgtcttttg cccggatccc 30600 cactatctgt gaaatgtcaa acctgttcgt tacctttaca aaatacatag ctatatcata 30660 cgtaaaaata tgttaacgaa agatcgttca ctctttcttc ggaaagataa ccaaagaatt 30720 aggaggatat ggtgcgcaca tagaaagggc cggtaagtaa ctctggcctt catagaacaa 30780 tatgttataa tttggaaatt gtcggatagc gcgactatgt gcttaattgg tttaccttaa 30840 attttataat tgatgacaat aaaaattcac cggagaagtt ctaacgtaag tatatagtta 30900 taactgaacc attcgaagac ttactccctt aacttaatta gacctatttg ttttccctga 30960 ctcctaagtt ctatggttgg caatttatct acaaggagac acgtatcatt taaaaacaaa 31020 aaattggaaa cttccttagc tgttgtgctg ggaacgctca aatcagtact cttcttctct 31080 agaagttgca aggggggaat tttatcatgt cgaagtttgt tataggagtc attcaaccat 31140 aggctccatg atactaaaag tgctggtaaa ctacgggagt aagatacata tttctcgata 31200 atagaattca gaattcgaat gagtccttgg tcgactcaga tccgaatcga gagaacccaa 31260 cgaatatata atttttatta catatgttgt cttagacaga gtattcctta acatcaactg 31320 gttttcagat aataagtaac tgcaacagag tcaaatcata gatattgata gtagttagag 31380 aaccattgat aatgttacaa ccattttcta attttgatgt tttgtagaca attattagtt 31440 cagcgagcta tctaacagtt cttacatatc tctactcatg caataaattc tgcaagttcg 31500 acactaactc aacctaggta acatcaaata cggatacatt caggggttat tatgcaaaaa 31560 atgggcttag ggataataga cactttacaa attgcgcatg caatcgagat attttatgta 31620 tcgatataat atgcattttt atagaattgc ctcctagcgt aacataaaca aacctatcta 31680 tttgcttttt tatccacgca tattacgtcc gtaccatttc cggccattca ttaagatcgg 31740 aagtaccttg ttatacaaaa ttaaaccttt aataccctat cgcgcagatt taggaattaa 31800 tttaatcaac tttagtggat caattataga catttttaag tggtttcttc aaggtagcat 31860 ttaaatatca atgatgactt actaaggttc taaatgagag aattcaatta atctggttag 31920 acaaagggat ccgaagatat tttatgccaa ccattaaaag ggcgttcctc tgtctacaat 31980 aagcctttat ttttaaatct ttgaagaaat cgagaacacg actgttgtca atacaaatat 32040 gataagaaga gaacttcatc tttgcccccc aataatagta cagcttcaaa taatattcac 32100 agtaaaatgg tatgtctggt actaagactt tcatgactgt tcgacgttct tattttatgt 32160 ataaagagta gtgatcccat gtctggagat tattctacat ccaattgact ctaagcacag 32220 ccttcttggg ttgcttgtat attgaaaatg atatactcaa ctggacgaga ctcataagga 32280 attatgattg accataagac cgctatgtat cgacgtcgtc ttagtttagt actcataatt 32340 atcatcattc ttttagtgcc tattacaatg ttggtaaaag aataaaactg catcacactt 32400 cttgataatg aacttgctcg attgattgtt gagaaagttt agtgatgagt atgttattta 32460 aggcactcgt gttctgatag agttggatcc attgttaatt acgagtatat aactcttcaa 32520 taattcgctc tatacgcaaa ttcctattat ctgtgaaatg tttaacgcgt acggtaactc 32580 tataaaatat ttatctacgt tataccttag aatatcttaa cggacaatcg caatatatct 32640 acttgtactg attaatggga aagtaggtgc gaatattgaa ggtatggtaa acgccggtaa 32700 gtaattctag gcaacaagga atgttatttt tttaattata ttttaaggga ttgtacgtct 32760 aaatcattaa taaaatttgt tgaaatcacc ttgtttgaat ttgtaaaaat tcgtcttaaa 32820 actttcaccg ttaattaatg attactacag tataattcca gaatttacat tcttaagcta 32880 attaaactaa tctgtttccc taggcttcta taaaatcgcg tcagtgatta tcccccatgg 32940 agacggatgt ttttcgcaaa taataattta caaatttcta tgtcacttct gctgtcaaca 33000 gttttgttta tactattctt gttatgatgt agattcgtcg cgttattatc atgtcgaact 33060 tcataaaaag tgtcttttta ccatgcagat caagattctg aaagtacaga caagcagcaa 33120 atacgaaatt catatttttg atcacaaggc cacagacctc tagtaagatg tacgttaact 33180 gaggttcgtg tcggtagtac caaacgttca tgtaactctc acaatgcgaa ttgacctgct 33240 ttgagctctt ctatatacta actggtattc tggcgaaaca aagctgtggt agtattatgt 33300 catgagtatt aatagtagta agaaaatcag ggataatgtt tcaacggttt acttatttag 33360 aggtagtgtg aggtactttt acgtgaacta gcaaactaac atctctttta aattattact 33420 cttacaataa gttccgtgac gacatgatta tctcaacctg atatacaatt gatgttctta 33480 tattgaaaag ttattaagcg agatatgtat ttaagcatag tagacacatc acaagttgcg 33540 gatatcatta agacaattta taaatagttc cattgtggaa tcttataata taacctgata 33600 gcgttatatt ggtgaagatg gctaattacc ctctcaccgg cgctattagc ttccatacca 33660 attggcgcca ttcattaaga tccgttgttc cttacgaaaa aaaaatttat aagaaatcct 33720 gtaacatgcg gatttagtag tttttattaa caacattact ggagcaaact aaaacattta 33780 aagacagaat tttggcagag gcaattaata tcatatgatg tgaaattagg gtctatattg 33840 taataatttg ataaattaaa ttagataagg ggtttggaag ataatttagc acagttagtt 33900 ataggcggaa gatttgcctt caaaaagcga ttattgttat ttgtcttaag ttacagcgaa 33960 aacagcagtc gttaaaacaa aaatgatatg tccaatatta catctaagca aggcaatttc 34020 gcaacaggtt aaggtatttt ttatagaaaa attaaaggtc cagttcaaac actttcattt 34080 gtgtagctcg tttatgcata tggtatgtaa actagtgcgt cggtgtatgg agatcttcct 34140 tcttgcgtat gattgcatgg actgcgatca cgttttgtaa gttcattgag actgttacgc 34200 ttaactgctc gaaattcgag aaaatatacg actgttcgta agagcgcttt gatcgaacac 34260 gatcataaca gagcacacat gataatcatc acttttttag tccctattac aaagcagcca 34320 agtgaatata tgtctatcac tcactatgaa tttgcatatg atcgttttat tgtagaaata 34380 atttaataaa tagaatgtta atgaagggtc tactgtactt atacaattgg gctacatgtt 34440 aactacaaga ctatatccta ttattgactt agtgtataca taatatggtt tcatatatgt 34500 agtgttcaag ggctatagca aatctgatga taaatgatta gggtaacacc ttagaataat 34560 atactagcct atcggaacac aagcacttct gccgtataat ccgagtgtgg ccacgataat 34620 ggaaggtatg gtcgaccgtt gttgctaata gtaggcgata acctatcctt ttttcataaa 34680 ttttctttag aacattgttc acataatata tcaggattaa ttgatgtaat gagctcgctt 34740 gattttgaaa acttgtgttt ttaattcgtc tccgattaat acactatact aaattttaaa 34800 gccagatata tcaatattaa actatttaat ttaacctaat cctcaaacgt tctattaaac 34860 cgtgtcaatc aatatctgct tcctaaatga aactttttcg ccaaaatcaa tagttagaat 34920 ctaaagtcgc tttcgtcctt agctatattg atttctttat acaggtagta acgtagattc 34980 attccgtaaa acggtttttc atttggatat aaaacatttc tttgatctcc aggagaaata 35040 tgagaaacta aacacgtcga gtaaatacgt atactgctca tttgtccacg cacccacata 35100 cctctggaag gaaaaacaca tactaaccta cctgacgcta gtgtaaaata tccaagagac 35160 cctgacagtg cgatttaacg agcctttaac tctttcatta gctgataagc attattgcga 35220 aactagcttg agcttgtatt gtccagtgtg tagtattaga agtgaaaaaa tcagggttaa 35280 taactcgtgg cgtcatttac atactgttag ccagtgacag gtaaacgtat actagcataa 35340 cgatatctta attaaattat ttatcttaca cattcttcct agatgacatg aatatgctgg 35400 tgcgatgtat aattgaactt ctgatatagg ataagaactg gaacacatat gtattatact 35460 aatgcatgta catcctaagt tttcaatatc gtttagagta caatttacaa atccctttcc 35520 ggaatcataa tataagatcg gatagccttg tgttcgtgga aagggcacat tagggtcaca 35580 gtggtggtat tacttatcaa actagctagc aataacgata atcatgtact actgaacagg 35640 aaaattgtat ttaaaagaag acttataact aatgttttac atagtcctaa ttgattacat 35700 tactcggggg aactaaaacc catgaccact aaaataaagc tgagtctaat tatgtgattt 35760 gatttaaaat ttcgatctat ataattataa tttcgagaga taaattggat tagctatttg 35820 ctagatgatt tggcacagaa tgacacagac gaagaactta ctatgaaata gcggtttcag 35880 ttgtcaatga tggatttcag cgaaactagg aaacgatgta gcttaagaaa tatctctctc 35940 attgcattta gataaagcat cttgctaaaa agagaaccag tactttgtaa agtaactaga 36000 ggttctcttt atactctgtg atttgtccag ttcttaaaag caaacgagga gtaatcaggt 36060 gcgtgggtaa aagcagacct tcatttttaa gtatgattag atggactacg attacatttt 36120 atgggatctc tgcgactgtc acgctaaaac actcggaaaa tgacaatcca atccacaact 36180 agttataacc cactgatcga aatggagcat aacgggtcac acatcataaa tttcactatt 36240 ttagtccgaa tttttcagca ttgcggtaga tgtgtaacaa ccgaccactg accatctaca 36300 tattattata ttccataaga tttaacttaa tatatagaaa acgcaagtag gatctactgt 36360 acttatatca ccatgctaca tattaagatg taagctaaat ccaattcttg tccttgagca 36420 tatatatcaa aaatacgaac attttggatt caaaagttat agcaaatccc accctaaacg 36480 tttggggaat ggttttagta ttatattcta gcctattaga acagaagcac cattcccatg 36540 taaacccagc gtcttcatca cattgaatac tttcatcgat taatattgtt tttaatatat 36600 gatgactcga ccttctaaca taaattttct actgattatt tataacaata tgtaccagga 36660 ttaaccaagg tactgagcgc ccatggtttt gggtactggt gattttattt cgactcaaat 36720 ttgcgcacta aagtaaattt taaagttaga catacagata ttataggtct ctatatgacg 36780 taaacgataa tcaaatagct aaaccgtatg ttactgagtc tgcttcttga gtgatacttt 36840 agccccttag tctacggtta ctagcaaaac tagttttgtt ctttcgatac accgaattgt 36900 ttatgaagag agtaacataa atctattttg tagaacgact ttattctagg tcatgaaaca 36960 tttcgttcat ctctaagaat attacaagac atcaagtagc ccaagatttt tcgtttgctc 37020 ctgattaatc cacgcaccca ttttcggatc gatgtaaaaa ttcattttag tctatttgat 37080 actattcaaa tataccctag gcacgcagtc agacgcatta tgagactctt ttactgttag 37140 gttaggtgtt gttcgatact gggtgtctgc caatacctca tattgcccac agtgtcgaat 37200 ttaaatcggt caaattaggt cctcaaagtt gtaacgccat ctccacattg ttgggtggtg 37260 accggtaggt gtataataat ataaggtatt gaaaattgaa ttaaatatac tttgcgcccg 37320 ttctacatga aataaattca gtcgaacgat gcaagaatct acattcgaac caagttatga 37380 gcgcgaactc ctatttaaag tttttatcgt tcaaaaacct gagagtttaa caacgtttta 37440 cgtgggacat gcaaacccct taccaatgtc gaaatatagg aatgaacaaa tctgtcttcg 37500 tgctaagggt acaattagga cgctgaagta atgtaactta tatgcaaggc aaattataac 37560 aaaaattaaa ttccagtaag gtggaagatt gtgatttaaa gatgactaat aaatattgtt 37620 atacatcctc ctaatcggtt ctacggttta cgagcaccaa aatccaagac ctctaaaata 37680 aagctaagtt tagacgcgtg attatactta agatatcaaa ctgttcgtct attatatcca 37740 gagatattct gtatctctta ttaatttatc gatctcgtat acaatgactc atttatagaa 37800 ctcagtatag aatctaagga tcgggtgcca atgatccttc cgatcagaac atgaaagcta 37860 tgtggtctga cgattacttc tctcaatgtc tttagaaaaa aaatcccgct gcaataggat 37920 ccagtactta gtaaagcagg ttgtgaatct tataatgttt tgaagatcat tggattttaa 37980 aaaccaaacg tggactgatt agaaacgtaa gctaaaatct agccacattt ctaactagtt 38040 tcagataaat tataataggt ttatatggta tgcgtacccc attctgcata atactttaag 38100 agaaagacaa tccaatgttg atcaagcttt gaattacaaa cgattatgat gtaatagggg 38160 tgttatggct tatattaagc cagaaatttt taggagtatc aacattgccc ttcggctcta 38220 ataactcact agtggtcatc gacatgataa tatattcgat aatttttaac taattttatt 38280 tgtatggcgg gcataatata tttagtttga gtctgcttgc aacgatgtta gacataagct 38340 tggttcaata ctcgcgctta aagaataatt tcaaaaacag cgagtgtttg gtgtcccaaa 38400 tagttactaa atgcaccctg tacgctagca taacggttac tactttatat ccattcttca 38460 atagacagaa agaagattct ctcgttaatt ttccaacttc tttacattgt atatacgttc 38520 cctttaattt cctattagaa ttaaggacat tccatcatct aacactaaat ttctactgat 38580 ttattataac aacatgtagg tggattaggc aagacccgaa atgctggttg tctttggttc 38640 tggatatttt ttctcgaact atatctgcgc gttaatatga attctataga tctacaagca 38700 gaaaagataa gtcggtttaa gacaatggga aaagttagat acctagagta ttaattattg 38760 agtaaatatc ttctcatata tgatagattc ctaccccccg gttgctagga aggttagtct 38820 tgtagtttcc atagacctga ctgctaaaga agagagtttc tgatatcttt tttttagggc 38880 gacgtaatcc tagatggtga ttcaaatgga ccaacattta gaacattaca attcttctag 38940 ttacctagta tttttatttt gcacctggga aatcattgct tttgattcta gatctgtgta 39000 aagattgatc agacttgaat taaatttgtc caaattatcc atgtgcatga gacaacatat 39060 attatgaaat tcacttttca aaaggtcaca actagttgga gacttattgt ttgctaatat 39120 tacattatcc tcacattagc gattgtagtt ggctctctaa aaatcgtcat agtaccaatg 39180 gggagccgag attattgagt gattaccagt agctaaacta tttaataagc tattaaagat 39240 tgatcaaagt aaacataccg cctgaaatat ataaatcata gtcagaccaa cattgctaca 39300 attcgtattc gcaccaactt ttgaccgcgt atttcccatt taagcttttg ttgctcacaa 39360 aacacttggt atacctatca atctcatggg acatgcgatc gtactgtgta cgaaggtatt 39420 tcggtaagaa gttatgtcac ttttatctaa gtaagcaaat aaaaagataa aaaaatgcaa 39480 tatatatgca agggaaatta atggacgatc ttaattcctg taaggtaaca cattgtgact 39540 caaaaatcat taaataatat aattgtacat ctggctaatc cgttctgggc ttttcaacca 39600 acagtaacgg agacttatta aaaagatttt gatacggacg cgcaactatt gttaggtaac 39660 tttgaagtcc gtcttttcga atcagccaaa ttcacttacc tttttgaatc ttcggatgtc 39720 ataattaata gctcgtttat agaagagtat acactatcta agggtggagg gccaagaatc 39780 cttccaatta ctacattaaa gatatctgca ctgacgtttt ctcttctcaa ggacaaaagt 39840 aaaaaaatgc cattgcacta ggatttaccg caaagattac ctcgttgtat atattgcagt 39900 cttaagaaca tcaatggatc ataaaaataa aacatgcatc ctttactatt aaaatctaag 39960 ttctagacaa attaccaatt agtctgaact tacattaaag agatttaata ggtacacgat 40020 ctctgtcgta taaattactt taactgaaat gttttccagt attaatcaat ccctgaatag 40080 catacaatta taatgagact agactataaa cgttagcaac gacagagaga ttattagcag 40140 catcatagtt acctctccgc tctagtatca cacaaatggt cgtcgatcag atagattggt 40200 cgataatctc catgaactat cgtctgtatg gcggacttta taaatttagt atcagtctgg 40260 tttaaatgat gttgagcatt agtgtgctta aaaaccgtca cataaagcgt atattcgaaa 40320 acaacgagtg tcttgtgaac catatgcata attagagtac tctgtagact agtatgacac 40380 atggctccac aaagctattt atcgatacaa tataaataga tatattcggc tatttttctg 40440 tttatatccg taatatttac gttgttttta attacttgcc agagttaaag acattccata 40500 gtgtggaact gggttttgag tttttcacta tataaacttg atgactactt tggctagtcc 40560 cgacaagtta gttctgaatg ccctcgaata atacttctaa aacaatgcct ggctgttgac 40620 agcagtgcat tgaaacttca ggcagaaaag cctagccggc ttaaccaagt ggaaaaactt 40680 agaagcgttc tattatgatt atcgaggaaa tatattctca tttgtgacag atttccacct 40740 ccggatactt agaaaggtta taggaataat tactattgag gtgagtacag tggagatgat 40800 atcatgttta cattatatga cggtaacgtg atcctgaatg ccgtttatga tagagtaatg 40860 tatttaacgt cggaattcct gtagttatct agtatttata tttcgaatat aggagataac 40920 attttaacat tcaagaattg tttcatgcat aatcagtctt gaatgtaatt actctaattc 40980 atcaatgtgt caaagacagt atatttaatc aagtaaagtt tagaaaaaga cataatcagt 41040 tacggagtta tcctatctta atattactct gatgtaatat ttgcaatcgc tgctgtctct 41100 ccaacattcg ccgtaatatc aatagagagg caagctcttg ctatgttttt caagggcatg 41160 tctaactatc caatcataag agggactcga tagcagacgt actgcctgaa atatcatcat 41220 gttagtgaaa ttaaattaag taccagtcgt aaagacacga ttttttagca gtttatttcg 41280 catataagct tttgttactt acacgatact tggtattcgt aatgatctca tgaggcgtct 41340 gttcatactg tattccgagg cgctccgata aattgctatg atatagttat ttatattagt 41400 cgataaaaag acaaatatag cgattaaaaa tgcaacaaat attaacgaac ggtctcaaat 41460 agtagaagat atccctcctc cggccatctc ctaaaatgcc atatattcgg attactgatg 41520 atgctcatca gggcagtttt atctagtctt ataggtgttt attttgaggt tattgttacc 41580 gaccgagaac cgtcgtgtcg tagctttgaa ctccgtgttt ccggattgtc tgacttggtt 41640 cacctttttg aatcttcgta ggattatact aattgacact ttttctaaaa gtaaacactc 41700 cctataggta gtggtcaatg agtttttgca ttatccttat tattgataat tctactcttg 41760 tcacgtgtat tacagtacaa acttattata ccaccatacc actagatctt atggagaata 41820 ctctctcaat acataaattg gagccacaga gacatgtata gatgataaat atagagatta 41880 tgtcctcaat tgtataattg tgagttctta acaaagaagg tatatgtcag aactttcact 41940 aatgacatta ggtatttgga cagtttcagt gatatagatt aattcatttc agatcttttt 42000 acgtattagt taatgcctca atagagttga attataataa cactatatta taaacgtcag 42060 cgacgacaga gaggttgaaa gcggcattct agtaatttgt tcgtttgaga acaatataaa 42120 atgttcctgt acagattgat gggatagtgt tctccctagg ctgtccactc cataatcgtc 42180 cttatagtag tacagtcgct ttaatttaat acgtggtcag cattttagtg ccaaataatc 42240 gtcaaattga acgcatatca gattgcgatg tatgtgctat ggacctttag cattactaga 42300 atcattccca aacaattttg ccataaggct ccgccacgca attaaacgat accataccaa 42360 taagaataat gtgccaaatc tttgttaata tcaccaattc ttacgttgat caataatgct 42420 tgccggagct tatcatcttt taaaggaagg acgcccgaag aggactttac ggtaaatatg 42480 cccaataact tcggccaatg atgctgtcta aataggttgg agtttccaga tataaggctc 42540 gaataagaat cggttataca acgcagcaca gggtcgaaac ttacgcaatt gtggcctaac 42600 agactgaatc aagtcgaaaa tctaaggagt aacccaatat gattgactgt gaataagaaa 42660 tttattcctg gggaataacc ctcaccattt actctaaacc ctaataggaa taaagattac 42720 taaaatgggg agagcgtact taatgtcatg ctcgaataaa atggtcctat agtgaactag 42780 ttcacctcct atgagaaagt taagaactta acctggatgt ctctacttac ataatctacc 42840 tgcatgcgta atacacgagt caacatatta acacacaaaa gttccttcct ccatatgcag 42900 ttaagcaagt catagctgta ttccataaac ctttcaaagt cactgtatct ggttatctaa 42960 agtctagaga aatgggtagt catttatggt aatatctcaa ttaagtttaa atgcgataaa 43020 ttgtgtacca tgctaatcta tgacgacttc gactctcttg ctgaaatttt ccacttcctt 43080 atgtggcctg acgaatactt ctctcattgt ttttagataa aagatcttgc tgaaatagga 43140 tccagtactt tgtaaagcaa gttgagaatc ttataatgtt ttgaagatca tcgggttcta 43200 aaaaccaaac gtggactaat taggtgcgtg ggtaaaaatc tagctacatt tctaagtagt 43260 ttcagataaa ttatgataag tttatatggg atgcgtacgc cagtctgcat aatactttga 43320 gagaatgaca atccaatcta gatcaagcta tgaattacaa acggttatga agtaaaacgg 43380 gtgttacagc ttaaatttag ccagaataat ttaggagttt caacattgcc gtagaggtgt 43440 aacaactcac cagtggccat ccacatatta atatattcca taacttttaa ctaattttat 43500 ttgtaacgcg ggcaaaatat attttgttta agtctgcttg ctacgatctt agacataagc 43560 ttggttcaat actcgcgctt aaagaaaaat ttcaaaaaca gcaagttttt ggactctcaa 43620 attgttacaa aatgcaccct gtacgttagg ggaatggtta ctactttata tccattcttg 43680 tttagacaga agcacgattc ccatgttaat cctccaactt cattacattg aatatacgtt 43740 ccctttaatt ttctatttga tttaaggtca ttccatcatc taacactaaa tttctactga 43800 ttatttataa caatatgtag gaggattagg caagatgcga aatgctggtt gtttttggtt 43860 ctggacattt tatctcgaat taaatctgcg cattaatatg aattctatag attgacaagc 43920 agaaaacata ggtctcttta agacaatggg aataattaaa tacctagagc ataaattact 43980 gagtaaatat cttgactcat atcatagatt cctagccccc ggttgctagg aaggttagtc 44040 ttgtagtttc catagaccag actgctaaag aagagagtta ctgatatctt ttttttaggg 44100 cgacgtaatc ctagatgatg attcatatgg accaacactt agaatattac aaatcttcta 44160 gttacctaat atttttgatt tgcacctggg aaatcattgc attcgatttt agatcggtgt 44220 aaagattgat caaactccat ttaattttat ccaaatttac catatgcatg ggataacacg 44280 tattatgaaa ttcacttttc gttaggttac aactagttcg agacttattg tttgctaata 44340 ctacattatc ctcacattag cgaatgtaat tggctctcta aaaatcctca tagttgtaat 44400 gggaagccga gattattgag tgatcaccag tagctgtact attttataag ctattaaaaa 44460 ttgattaaaa taaacatacc gcctgtaata tataaatcat agtcagacga acattgctac 44520 aatctgtatt cgtaccaagt tatgagcgcg tatttcttat ttaagttttt gtcgctcaca 44580 aaacactggg tttaccaatc attcacatgg gacatgcgat cgtactgcct atgatgatat 44640 attggtaaga agttatctgt ctttcttcta agagagcaat taaaaggtta aagaaatgca 44700 atatatatgc aagggaaatt aatggataat cttaattcct gtaaggtagc acattgtgac 44760 ttaaaaatga ctaaataata ttgttgtaca tctagctaat ccgttctggg ctttacgacc 44820 aacagtaacg aagacctatt aaaaagacct tgatatggac gcgcaactat agttaagtta 44880 cctagatgtc cgtcttttct attcagccaa attctcttac ctttttcaat ctttggatct 44940 cataattaat agctcgttta tagaagagta tatactatct aagggtggag ggccaaggat 45000 ccttccaatc acaacatcaa agatatctgg actgacgttt tctcctctca aggactatag 45060 aaaaaaaatc ccattgcatt aggatctacc acaaagatta cctggttgta aattttgcaa 45120 tgttaagaac atcaatggat cataaaaata aaacgtgcat cctttagtaa caaaatctaa 45180 gatctagaca aatttccaac tagtctgaac ttagattaaa gaggtttaat aggtacacga 45240 actctgtcgt atatattact ttaagtgaaa tgttttccag tgttgatcaa tctctgaata 45300 acaaacgatt ataatgaaat tagagtgtaa acgttaacaa cgaccgagag atttttagca 45360 gcatcatagt tacctctcgg ctctagtatc tcacaaatgg tcatcgattt gataaattgc 45420 tcgataattt ctatgtactt tcatttgtat ggcggacttt ataaatttag tatcagtctg 45480 gtttaaacga tgttgagcat aagtgtgctt gaaaactgcc acataaaggg taaattcgaa 45540 aacaacgagt gttttgtgaa ccatatgcat aattagagta ccctgtacgc tagcatgaca 45600 catgcctcca caaagctatt cttcaataca gtataaatag attcattcgt ctatttttct 45660 atttatatgc gttatattta cgttgtcttt aattacctgc tagaattaaa gacattccat 45720 tgtgtaggac tgagttttca gtaattcatt atattaactt gaagaccaat taggcaagac 45780 ccgacaagtt ggttgtgaat gcctccgaat aattcttcta aaactatgcc tgggtgttga 45840 cagcaatcca ttgaaacttc aggcagaaaa gcttagccgg tttaagtgaa tggaaaaact 45900 tagaagcgtt ctgtattgat tatcgaggaa atattttctc atttgtgaca gatttccacc 45960 tcccggttct taggaaggtt aaaggtgtaa ttactattga cgtgactgca gaggagaaga 46020 gatcttgttt tcatttttta acggtaacgt gatcctgaat ggcgtttata atggagcaat 46080 atatttaacg tcggaattct tgtagttatc tagtattttt attttgtata taggagataa 46140 taattttaca ttcaagattt gtttcatgct taatcagtct tgaatgtaat ttctctaaat 46200 catccatgtg ccagagacag catatttaat gaaataaact ttacaaaagg tcataattag 46260 ttagggagtt atcgtatgtt aatattactc tgatctgata tttgcaatcg ctgctgtctc 46320 tccaatattc gccgtaatat caatagagag gcgagatcat actgtgtttt ccaacggcaa 46380 gtctatctaa ccagttatta gaggaacttg atagcagaca taccgcctga aatatttaca 46440 tcttagtcag attaaattta ctacgactcg taaacacacg attttttggc agtgtatttc 46500 gcatataagc ttttgttgct tacaggacac ttggtatacg taatgatctc atgagacatc 46560 tgttcatact gtgtaccgag gtgttccgat aaatagctat gttatattta tctatataag 46620 ccgataaaaa gacaaatata ggcattaaaa atgcaacaaa tattaatgaa cggtctcaat 46680 tagtgaaagg tatcgctcct tggcccatat ctcaaaaagc gatatattcg aactactgat 46740 gaagcccatc agggctgttc tatctagact tacgggagct tattttgagg tttttgttac 46800 ggaccgacaa ccgtcgtgac gtaactttga agtccgtctt tccggatcga ccgaattggt 46860 tcaccttttt gaatcttcgt aggattatac taatagttgc tttttgtaaa agtaaacact 46920 ccctataggt ggtggtctat gagtttttcc attatcctta ttattgataa ttctactcat 46980 gtcacctcta ctatagtaca aatttattat actaccattg cactagatct tacggcgaat 47040 actctctcat tacataaatt ggagcctcag ggacatcaat agatgataaa tataaagttt 47100 atgtcctcaa ttgtaaaatt gtaagttctt aacaaagtac gtattagtca gaacttacat 47160 taatgacatt aggtatttac acagtttctg tgatatagat tagttcattt caaatctttt 47220 tccgtattag ttaatgcctc aatagaatag aattataata acactatatt ataaacgtta 47280 gcgacgacag agaggttgaa agcggcattc tagtaatctg tccgttcgag aacgatataa 47340 aatgttcccg tacagattga taggttagta ttctccctga gctgtcgtct ccatgacgga 47400 ctttatagta gtacaatcgc tttaatttaa tacatggtca gcatttctgt gccaaaaaat 47460 cgtcaaataa agcgtatatc cgaatacaat gaatgtgcta tggaccataa gcattactag 47520 aatactccgc agacaactat gtcataaggc tccgcgacgc aatttaacga tactatatca 47580 ataaaaataa tcagctattt ttctgtttat atcgctaatt cttacgttga ttaatattgc 47640 ttgccggagt ttatcatctt ttatagggag gaggcccgta gaggatttta cggtatataa 47700 gcctaataac ttctaccaat ggtgccgtca aaatagatca gagtatccag aaataaaact 47760 ccaataagaa tggctgacac taggcagcac aggatcgaaa cttacgctac aaaggcctaa 47820 cagactgaac caagtggaaa aactaagaag tatcccaata tgattaactg tgaataagat 47880 attcattcgt gaggaatatc cgtcaccact tactcaaaac cgtaatagga ataataacta 47940 ttaaaatgag gacagcgtac ataatgtcat gctcgaataa tatggtcgta tggtgaacta 48000 gaatacctcc tatgagaaag ttaagaactt aacctgggtg tctctgttca tatcttctat 48060 ttgcatctgt aatacaggag tcaacatatt aacacacaag agttctttct tccatatgca 48120 gttatgtaag tcattgctgt attccataaa cctgtcaaag tcactatatc tggttatgta 48180 aagtctagag aaatgcatag tcatttatgg aaatatctca actaagtata attgtgataa 48240 aatgtatgta gtggctgctg tttttccaac tttcaccgtg agatgattaa acaagtacac 48300 tcttgttatt tattataagc tcatgtgtaa ctaccctatc acaagagcga tccgacaggt 48360 gaagcattaa caggaaactc atcacgtcag cgtaattaaa ttatacacca gtcgtaaaat 48420 cgcactttat tagcagttta acatgcgtat agtctaaccc tacatacacg atacctgaaa 48480 atcgtaatga acttagtaac ggatagttaa aacggtattc cgaagcggtg cattaattcg 48540 ctaaggtatg gttgttctta ttacacggct tagatacaat tatagtggca aggaatacaa 48600 ttagttatta ccaacggcga cgaatagtag tttatttcct tcctgcgggc tcctcctgaa 48660 atgccactta tacggcttat tgaagccgat tagtacaata gatttattca acctcaaagg 48720 tctatattcc cggcttattc ttagctgata tgtcgcgtcg agtcccgggt ttgagtgcgt 48780 taacaccgga ttgtctggct tagttcaact tttggatacc tcatcgggtt atactaactg 48840 acacttattc tttaaataag gttcccttat tgggactagt aactgagatt tgaaattatc 48900 cttatttcta atgatcttaa ctctttcgca tgaataacag tacgagctta ctttacgagg 48960 ttatcactta ttcaagtgca ggatactctt ccaattcttg aaaaggacgt agagagttga 49020 atgtattaga tgggcgtacg cattatgtgc tcacttgtat aattgtgtgt tttcaaggaa 49080 ggaagtatgc atcaattcgt tcagtatcga cataaggtat ttggaaagtt ttagtgacat 49140 agtccaatag atttttgatc tctttaccga tcagtaaata ccattataga gtcaattcag 49200 atttacgcta tttaacacac aacgccgagg ataaaagggc tgaaattgac agtctacaat 49260 attgttcatg tgtcaacaat taacaataaa cgaatacacg ttgatgcgat agtgctctgg 49320 ctaggccgtc cacttcgtat attttctttg agtagtgtag acgtattaat atagcatgta 49380 gccagattct tagcatgaaa cgatgattaa aatgtacgca taccacgttg agatgtatgt 49440 gctatagact tttagtgtta cctgaaacat agtccatcga ttttgccata agactacggc 49500 acgaaattaa gtgatttcac acctacaaga gaaatgtgcc gaatctatga taataacacc 49560 gttcgttgaa ttaatcaata atggtagcgg ttgcatatta tcaaatgaag gagccacacc 49620 cggggaggag tttacggtcg atatcccgta caacttcggc taatcatgtt atctaaataa 49680 gttggggttt gcagttgtca gggcgaaatc agattcgact atacagccca gttcagagcc 49740 ctaactcacg taatcgtgac cttacagagc gagtcaagtt aattacctat cgagtagctc 49800 aatatgagag agtgtgaata agaaatttat tgcaaggcaa taatcctaat tattgatagc 49860 aaactttgat aagaataaag aatagcacaa ttcagaaagc gtacttactg tcatactcga 49920 atgaaatgct ttattaaaga aaaagttgac gtcctatgag aagattaaga acttctcctg 49980 catctcacaa cctacttaat cttcggggat gggtaataga taggtgaaca tataaataga 50040 gataagtttc taccttcaaa cgtagttaaa caagtcatag ctatatccca caaacctttc 50100 ataatcactg tatcgggata tctaagaagt aatggtatgg ctagtgatca atcataattt 50160 gtcagttaag acaagatgcg gtaaattgtg tattgcgagt cctattgtct cgacttaaac 50220 tgtctgatgt tataacaaat acacagtagt taattgtcaa ttgcttatgt actgctacgc 50280 cttgtggaga ccggtccggc gcgtgaagga tataaatgaa actcgtcaca tccacattat 50340 tatatcgtac atcggagtac gaatcgaact tggcctctat ttttagatgc gtgcgctgca 50400 agtgtaaatg taccaaatct aaaaattacg atgggttttg tttcaggtat ccaaaaagta 50460 attctgatgc cgcgcttcaa ttcactaaag tgaggatgtt gtctttatac gggtttgata 50520 cttttattgt accaagcatc tttattagtt agcactatcc ccaacgtata acagtttact 50580 ttctcggtgt gagcccctcg tcaactgcct gcagtagtgc acgttgaagc cgattagaat 50640 attagatttt ttcaactcca aacgtcaaca gtcccgctct agtttaagtt catatatcag 50700 gacaggtctc ggaattaagt gtataagcgc tgggaacttt cgcgcagttc gaatagtgag 50760 taagtgatcg agttacacac cgttagattc atccttcaga taacgttccg tttttttgat 50820 ttataactat cgtttgaatc aattccatct tcttaacgtg ttaagtcatc cgcaaaaaag 50880 acgatatgag cttaccttac gaaataattt catttcgaac tacagggtgc tctacttgct 50940 cttgaagata tcgtagagtg ttggatgaat taaaaccccc tacccatttt caatcgactt 51000 gtatgtttat ccttaaacta acatggaaat ttgctttgat ttgtttagta tcatttaggc 51060 gtgtttcaaa tgtataatta agtatgccgc atagtttctc cattaccgaa ctgaacacca 51120 cttagtgtca aacactcatt gctgttctac gccatttatg acattacgct caaattaact 51180 gagctgaatt tcaaagacta taatattatt tttgtgttac caattaacag ttaacgaata 51240 cgtgacgatg tagaacacca ggagccaggt cggacgcatc ctatatttac ttcgtgcaga 51300 gtaggtctaa caagatagca tggaggttta tgcttaagtt gaaccgcaga taaaggtcta 51360 tactcgggat cttcactatc tgaaggttta gaattttaat ggtaccctat acaaagtgca 51420 taggtttttc attaatatta agacgcaaag gtaagtgaat tcactcctac aacggaaata 51480 tgaccaaagt atcaaaacaa tatggttcgt agaaaaaatc gatcgtgata aaggttggat 51540 tttgacaaaa caaagagcta cagtctggga acagtcgatg gacgccacca cgtgcttttt 51600 cggctaatct tataatttat aaatgttgag gtttagaatt ctcgggacga gatcaaattt 51660 aagtatatag tcatatccga agcgttaaat cacttattcg ccatgcttaa aagcgcctca 51720 tgcttttcac tgattcatta gctctatgta tggcaatcta tgtacgaagt ctgtcgtaag 51780 acttaaaaac taaatattgg tagcttcctt agttaaagta caaggattgc acaattcagt 51840 agacattttt tctggtattc ccgaacggaa agctttatta agacaaagtt tgatatccga 51900 cgggatgaac cacaagttct atgatatctc agaagctact aaatttcggg ggttgggtac 51960 aagttagttg aacatacaaa ttggaatttg attgtatcct tggtcgattc taaacaaatc 52020 gtagtaaacc cgtgcaaagt atatatatta tttcatacgg cgtttcaaag aggcaatggc 52080 ttgacatgag ctggatttca gattatgagt aaccacaaga tagtgtaaac actgtaatcc 52140 atatttaatt gagatctctt aaaaatgctg atattatttt ctaaactcga tggtttattg 52200 acaattattt gtacagtgat acatctagcg gttcttggtc gaccctactt acgtaataaa 52260 tgtagcacgt ttgatccaga ctcatccatg gtagcttcaa atacggattc aacttggcgt 52320 ttatatccag aaaacggccc tagaggtgat agacatttaa cactttggac aaccattgga 52380 tatgtttcac gtatcgatat agtaattatt attctgcatt tgctttctag taagtgagga 52440 agaagccttt ctattggttt cttaatcctc ttataccacg cgtgtatctt tcctagccct 52500 ttattgaaac tggaagtgtc ttgttattca atgttaaacc cttgtcagcc tattgcggtg 52560 atgcacgaaa aaaccaaatg gaatataaaa tattaactac tcctaatttt aagtgccctc 52620 ttctagattg tattcatata tcaatataga cttcgcaaac tagtgaatga gggaaatgaa 52680 tttatccgga ataacaaaag ggactgagta ttcaagatac aaaccgttag atagatgttc 52740 cagtctgcat agtaaatttt tgttttttaa ccattgaagg aatcaatttc acgatcctta 52800 cgagtttagt catcagaaaa agagaccttt agcgctcccc ccttgaaata gtacagcttc 52860 aaacaataac ctgagtaagt tggtatccga ggtactataa atttttcgat catttgatgc 52920 ccccatccta tgtttaaaga actattatct ttagccttaa gcttacacag gaaccagctg 52980 agtctaggtt aggttcactt gggttgctta catatataaa ataatgtata ccgcagaatt 53040 tgtcccttag ggaattgtag ttgaccaaaa gtctattact cattggcgtt gtatcacttt 53100 tgtgtcaata ggtatgatca atctcttgga aattattacg atgttggtaa aagattagaa 53160 ctactaaaca actgttaata atcaagtcgc tccgtagatt gccaagaatc agttgagatg 53220 aatacgttat ttagaacgta caaactacga ttgagttggt accatcgtag tttatgccta 53280 tgttaatccc caattatacg tcttttgccc gaatccccac tatctgtgaa atgtttaacg 53340 tgttcgttac ctttacaaaa tacatagcta tatcatacgt aaaaatatct taacggaaga 53400 tcgtacactc tttcttcgga tagataagca aagaaatagg aggatatggt gcacacatgg 53460 aaagggccgg taagtaattc tggccttcat agaacaatat gttataattt ggaaattgtc 53520 ggatagcgcg actatgtgct taattggttt actttaaatt ttataattga tgacaataaa 53580 aattcaccgg agaagttcta acgtaagtat atagttataa ctgaacgatt ccaagattta 53640 ctcccttaag ttaattagac ctatttgttt tccctgactc ctaagtttta tggttggcaa 53700 tttatctgca aggagacaca tatcatttag aaataaaaaa ttggaaactt ctttagctgt 53760 tgtgctggga acgctcatat caatactttt cttctctaga agttgaaagg ggggaatatt 53820 atcatgtcga agtttgttat aagagtcatt ctaccataca caccatgatt ctgaaagtgc 53880 tggtaaacta cgggaataag atacatattt ctcattatta gaattcagac ctcaaatgag 53940 tcgttggtca actcagatcc gaatcgaaag aacccaacga atatataact tttattacat 54000 gtgttgtctt agtcagagta ttccttaaca ttaactggtt ttcagacgat atgtaactgc 54060 agcagagtca aatcatggat attgatagta gttagagaac cattgataat gttacaacca 54120 ttttctaatt ttgatgttgt gtagacaatt attagttgag cgagctaact aacagttctt 54180 acaaatctct actcatacaa taaattccgt gagctcaaca ctaactcaac ctaggtaaca 54240 tcaaatgccg atatattcag gggttattat gcgagatatg cgcttaggga taatagacac 54300 tttacaaatt gcgcatgcga tcgagatatt ttatgaatcg atataatatg cattcttata 54360 gaattgcctg ttagcgtaac ataaataaac gtatctaatt acttttttat ccacgcttat 54420 tacatccgta ccatttccgg ccattcatta agatcggaag taccttgcaa tacaaaatta 54480 aactttaaat accctatcac gcagatttag caattatttt aatcaacttt agtggatcaa 54540 tcatagacat ttttaagtgg tattttcaag gtggcattta aatattaatg atgacatatt 54600 aaggttttaa atgagagaat tcaattaatt tggttagaca aagggatccg aagatatttt 54660 atcgcaacca ttaaaagggc gtacctctgt ctacaataag cctttatttt taaatgtttg 54720 aagaaatcgt gaagacgact gttgtcaata caaatatgat aagaacagta ctacatcttt 54780 gctccgcaat aatagtacag cttcaagtaa tattcacagt aaaatggtat gtctggtact 54840 aagactttca tgactgttcg acgtttatac tttaagtata aagagtagtg atccgatgtc 54900 tggagattat tctacatcca attgactcta agcacagcct tcttggcttg ctagtacatt 54960 gaaagtgata tactcaactg gacgagactc gaaaggaaat atgattgacc ataagaccgc 55020 tttgtttcga catcgtctta gtacagtact cataattatc atcattcttt tagtgcctat 55080 tacaatgttg gcaaaagaat aaatctccat cacacttctt gaaaatgtac ttggtcgttt 55140 gattgttgag aaagtttaat gatgagtatg ttatttaagg cactggtgta ctgatagagt 55200 tggactaaat gttaattacg agtatataac tcttcaataa ttcgctctat acgcaaattc 55260 ctattatctg tgaaatgttt aacgcctatg gtaactctgt aaaatattta tctacgttat 55320 accttagaat atcttaacgg actatcgcaa tatatctact tgtactgatt aatgggaaag 55380 taggtgcgat aatcgaaggt atggtaaacg ccggtaagta attctaggca acaaggaatg 55440 ttattttttt aattattttt taaggcattg tacgtctaaa tcatcaataa aatttgttga 55500 aatcaccttg tttgattttg taaaaatttg tcttaaaact atcaccgtta attaaagatt 55560 actacactat aattccagaa tttacattct taagctattt aaactaatct gtttccctag 55620 gcttctataa aatcgcgtca gtgattatcc cccttggaga cggatgtttt tcgcaaataa 55680 caattaacaa atttctatgt cacttctgct gtcagcaatt ttgtttttac tatacttgtt 55740 atgatgtaga ttcgtcgcgt tattgcgttg tcgaacttca taaaaagtgt ctttttacca 55800 tgcagatcaa gattgtgaaa gtatagacaa gcagcaaata cgaaaataca actacagttt 55860 tagcggtaaa gtctatctaa atttaataat gtcagtgtta tgtgactact ttttatattt 55920 cttcgcaacc tttattatcg ttgttttaaa ccaaattgcc acttacttct aaacgtcaat 55980 cagcctcttg gattacaaca atttagtcga taacttcgtc gatctcttag gatttttcat 56040 gttaatgctc tgatacattt ttgtagcctg atattttgtc tgtttctgcc ataacaactc 56100 gatccgacag gtgaggtatt agcaggaata tcatcatgtc agcgtaatta aattatgcac 56160 cagtcgtaaa atcacggttt attagcagtt taacatgcgt atagtctaac cctacataca 56220 cgatacctga aaatcgtaat gatcttagta agggattgtt aaaacggtat tccgaggcgg 56280 tgcattaatt tgctatggta tggttattct tattacacgg tttagaaaca attatagtgg 56340 ctaagaatgc aactagttat tacgaacggc cacgaatagt agaaaatttc cttcctgcgg 56400 gcttctcctg aaatgccatt tatacgggtt attgaagccg gttactacga cagatttatt 56460 caacctcaaa ggtctatatt cccagcttat tcttagccga tatgttgcgt cgtgtcccag 56520 ctttgaatgc gttaacaccg gattgtctga cttagttcag cttttggata cctcatcggg 56580 ttatactaac tgacacttat tctttaaata aggatccctt attgggagtg gtaagtgaga 56640 tttgggatta tccttatttc taatgatctt acccctttcg catgaataac agtacgagct 56700 tattttacga ggatatcact tgatcaagtg gaggatactc ttccaattct tgaataggac 56760 ctagagagtt gaatgtatta gatggacgta cgcattatgt gctcagttgt ataattgtgt 56820 gttttcaagg aaggaagtat gcgtcaattc gttcagtatc gacataaggt atttggaaag 56880 ttttagtgac atagaccaat agattttaga tctctttacc catcagtaaa taccattata 56940 gagtcaattc aaatttacgc tatttaacac acaacgccga ggataaaagg gctgaaattg 57000 acagtctact attttgttca tgtgtgaaca attaacaata ttcgaataca cgttgatgcg 57060 atagtgctct ggctaggctg tccacttcgt atattttctt tgagtagtgc agacgtatta 57120 atataatatg tagccaggat cttagcatga aacgatgatt aaaatgtacg cataccacgt 57180 tgggatgtat gtgctataga cttttagtat tacctgaaac atagtccatc gattttgcca 57240 taagacttcg gcacgaaatt aagtgatttc acacctacaa gagaaatgtg ccgaatctat 57300 gataatatca ccgttcgttg tattaatcaa taatggtagc ggctgcatat tatcaaatga 57360 aggagcgaca cccggggagg agtttacggt ggatatcccg tacaacttcg gctaatcatg 57420 ttatctaaat aagttggggt ttgcagttat gagggcgaaa tcagattcga ctatacagcg 57480 cagttcagag ccctaactca cgtaatcgtg accttacaga gcgagtcaag ttgataacct 57540 atcgagtagc tcaatatgag tgagtgtgaa taagaaattt attgcaaggc aataatccta 57600 atcattgaca gcaaactttg ataagaataa agattagtac aattcagaaa gcgtacttac 57660 tgtcatactc gaatgaaatg ctttattaaa gaaaaagttg acgtcctatg agaagattaa 57720 gaacttctcc tgcatctcac aacttacata atcttcgggc atgggtaata gataggtgaa 57780 catataaata cagaaaagtt tctaccttca tacgtagtta agcaagtcat agctatatcc 57840 cataaacctt tcataatcac tgtatcgggt tatctaagaa ctaatggtat ggctagtcat 57900 ttatggtaat ttgtcagtta agtcaagatg cggtaaattg tgtattgcgg ctcctattat 57960 cccgacttaa actgtctgat gttataacaa atacacagta gttaattgtt atttgcttat 58020 gtactgctac gctttgtgga gaccggtccg gcgcgtgaag catataaatg aaactcatca 58080 catccacata attatatcgt acatcggtgt aggaatcgaa cttggcttct aattttacat 58140 gcgtgcgctg caagtgtaaa tataccaaat ctaaaaatca cgatgggctt tgtttcaggt 58200 atctaaaagg aaattctgat gccgcgcttc aattcactaa agtgtggatg ttctctttat 58260 acgggtttga tacttttatt gtagcaagca tctttattag ttaatactat ccccaacgta 58320 taatagttta cttcctcggt gtgggcccct cgtcaagtgc ctgctataga gcatgttgaa 58380 gccgattagt ataatagatt ttttcaactc caaacgtcaa cagtcccgct ttagtttaag 58440 ttcatatatc gggacaagtc tcggaattaa gtgcataagc gctgggatct ttcgcgcagt 58500 tcgaataatg agtaactgat cgagttacac accgtcagac ttattcttca gataacgttc 58560 cgttattacg attaataact atcgtttgaa actattccat cttcttaacg tgttaagtct 58620 ttcgcaaaaa agacggtatg agcttacctt acgaaataat ttcttttcga actacagggt 58680 gctctacttg ctcttgaaga aatcgtagag tgttggatga attaaaagcc cctacccatt 58740 ttctatcgac ttgtatgttt atccttattc taacatggaa gtttgcattg atttgtttag 58800 tatcaattag gcgtgtttca aatgtattaa taagaatgcc gtatagattc tccattaccg 58860 aactgaacac tacttagtat taaacagtca ttgctgttct acgccattta agacattacg 58920 ctcaaaataa ctgagctgaa tttcagagac tataatatta tttatgtgtc accaattaac 58980 agttaacgaa tacgtgacga tgtggaacac caggagccag gtcggacgca tcctatattt 59040 actttgtgca gagtaggtct aacaaaatag catgtaggtt tatgcttaag ttgaaccgca 59100 gataaaggtc tatactcggg atcttcacta tctgaaggtt tagattttta atggtaccct 59160 aaacaaagtg cataggtttt tcattaaaat tatggcgcaa agctaagtga attcactcct 59220 acaacagaaa tatgaccaaa gtatcaaaac aacatggttc gtagaaaaaa tcaatcgtga 59280 taaaggttgg attttgacaa aacaaagagc cacactctgg gagcagtcga cggacgtcac 59340 cacgtgcatt ttcggctaat cttataatct ataaatgttg aggtttacag ttctcggggc 59400 gagatcaaat ttaagtatat agtcgtatcc gaagcgttaa atcacttatt cgccatgctt 59460 gaaagcgcct catgcttttc actgattcat tagctctatg tatggcaatc taagtacgaa 59520 gtctgtcgta aggcttaaaa actaaatatt ggtagctacc ttagttaagg tacaaggatt 59580 gcacaattca gtaggcgttt tttctgctat tctcgaacgg aaagctttat taagacaaag 59640 cttgatatcc cacgggatga accagaagtt ctatgatatc tcagaagcta ctaaatttcg 59700 ggggttgggt acaagttagt tgaacataca aataggaatt tgattgtatc cttagtcgat 59760 tctaaacaaa tcatagtaaa cccgtgcaaa gtatacatat taattcatac ggcgtttcaa 59820 agaggcaatg gcttgacttg agctgaatta cagattatga gtaaccacaa gatagagtaa 59880 acactgtaat ccatgtttaa ttgagaccac ttaaagatgc tgatattatt ttcaaaacac 59940 aatggtttat tgacaattat ttatacactg atacatcttg cggttcttgg tcgaccctac 60000 ttacgatata aatgaagcac gtttcatcca gattcatcta tcgtagctcc aaatacgaat 60060 tcaacttggc gtttatatcc agaaaagagc cctagaggtg atagactttt aagtctttgg 60120 actaccatgg gatatgtttc acgtatcgaa atagtaatta ttattctgcg tttgctttca 60180 tataagtgag gatgaagcct ttatattggt ttcttaatct tcttatacca cgcgtgtatc 60240 tttcctagcc ctttattgaa tctggaagtg tcttgttact caatgtcaaa cccttgtcag 60300 cctcttgcgg tgatgcacga aaaaaccgaa tggaatataa aatattaact actcctaatt 60360 ttaagtgccc tcttctagat tgaattcata tatcaatata gacttcgcaa actagtgaat 60420 gaggggtatg aatttatccg gaataacaaa agggactaag tattcgagat acaaaccgtt 60480 agatagatgc tccagtctgc atagtgaatt tttgattttt aaccattgaa ggaatcaatt 60540 tcacgatcct tacgagttaa gtcatcagaa aaagagacca ttagcgctcc ccctttgaaa 60600 tagtacagtt tcaaacaata gcctgagtaa gttggtattc gagatactat aaacttttcg 60660 atcatttgat gcccccaacc tatgtttaaa gaactattat ctttagcctt aaactaacac 60720 aggaaccagc taagtcttgt ttagcttcac ttgggctggt tacatatata aaataatgta 60780 tgccgcagaa tttgtccctt agcgaattgt agttgaccaa aagtctatta ctcattggcg 60840 ttgtatcact tttgtgtcaa taggtatgat taatctcttg aaaattttta cgacaatggt 60900 aaaagattag aactactaaa caactgttaa taatcatgtc gctccgtaga ttgccaagaa 60960 tcagttggga tgaatacgtt atttacaacg tacaaactac gactgagttg gtaccatcgt 61020 agtttatgcc tatgttgaac cgcaattata cgtcttttgc ccggatcccc actatctgtg 61080 aaatgtcaaa cctgttcgtt acctttacaa aatacatagc tatatcatac gtaaaaatat 61140 gttaacgaaa gatcgttcac tctttcttcg gaaagataac caaagaatta ggaggatatg 61200 gtgcgcacat agaaagggcc ggtaagtaac tctggccttc atagaacaat atgttataat 61260 ttggaaattg tcggatagcg cgactatgtg cttaattggt ttaccttaaa ttttataatt 61320 gatgacaata aaaattcacc ggagaagttc taacgtaagt atatagttat aactgaacca 61380 ttcgaagact tactccctta acttaattag acctatttgt tttccctgac tcctaagttc 61440 tatggttggc aatttatcta caaggagaca cgtatcattt aaaaacaaaa aattggaaac 61500 ttccttagct gttgtgctgg gaacgctcaa atcagtactc ttcttctcta gaagttgcaa 61560 ggggggaatt ttatcatgtc gaagtttgtt ataggagtca ttcaaccata ggctccatga 61620 tactaaaagt gctggtaaac tacgggagta agatacatat ttctcgataa tagaattcag 61680 aattcgaatg agtccttggt cgactcagat ccgaatcgag agaacccaac gaatatataa 61740 tttttattac atatgttgtc ttagacagag tattccttaa catcaactgg ttttcagata 61800 ataagtaact gcaacagagt caaatcatag atattgatag tagttagaga accattgata 61860 atgttacaac cattttctaa ttttgatgtt ttgtagacaa ttattagttc agcgagctat 61920 ctaacagttc ttacatatct ctactcatgc aataaattct gcaagttcga cactaactca 61980 acctaggtaa catcaaatac ggatacattc aggggttatt atgcaaaaaa tgggcttagg 62040 gataatagac actttacaaa ttgcgcatgc aatcgagata ttttatgtat cgatataata 62100 tgcattttta tagaattgcc tcctagcgta acataaacaa acctatctat ttgctttttt 62160 atccacgcat attacgtccg taccatttcc ggccattcat taagatcgga agtaccttgt 62220 tatacaaaat taaaccttta ataccctatc gcgcagattt aggaattaat ttaatcaact 62280 ttagtggatc aattatagac atttttaagt ggtttcttca aggtagcatt taaatatcaa 62340 tgatgactta ctaaggttct aaatgagaga attcaattaa tctggttaga caaagggatc 62400 cgaagatatt ttatgccaac cattaaaagg gcgttcctct gtctacaata agcctttatt 62460 tttaaatctt tgaagaaatc gagaacacga ctgttgtcaa tacaaatatg ataagaagag 62520 aacttcatct ttgcccccca ataatagtac agcttcaaat aatattcaca gtaaaatggt 62580 atgtctggta ctaagacttt catgactgtt cgacgttctt attttatgta taaagagtag 62640 tgatcccatg tctggagatt attctacatc caattgactc taagcacagc cttcttgggt 62700 tgcttgtata ttgaaaatga tatactcaac tggacgagac tcataaggaa ttatgattga 62760 ccataagacc gctatgtatc gacgtcgtct tagtttagta ctcataatta tcatcattct 62820 tttagtgcct attacaatgt tggtaaaaga ataaaactgc atcacacttc ttgataatga 62880 acttgctcga ttgattgttg agaaagttta gtgatgagta tgttatttaa ggcactcgtg 62940 ttctgataga gttggatcca ttgttaatta cgagtatata actcttcaat aattcgctct 63000 atacgcaaat tcctattatc tgtgaaatgt ttaacgcgta cggtaactct ataaaatatt 63060 tatctacgtt ataccttaga atatcttaac ggacaatcgc aatatatcta cttgtactga 63120 ttaatgggaa agtaggtgcg aatattgaag gtatggtaaa cgccggtaag taattctagg 63180 caacaaggaa tgttattttt ttaattatat tttaagggat tgtacgtcta aatcattaat 63240 aaaatttgtt gaaatcacct tgtttgaatt tgtaaaaatt cgtcttaaaa ctttcaccgt 63300 taattaatga ttactacagt ataattccag aatttacatt cttaagctaa ttaaactaat 63360 ctgtttccct aggcttctat aaaatcgcgt cagtgattat cccccatgga gacggatgtt 63420 tttcgcaaat aataatttac aaatttctat gtcacttctg ctgtcaacag ttttgtttat 63480 actattcttg ttatgatgta gattcgtcgc gttattatca tgtcgaactt cataaaaagt 63540 gtctttttac catgcagatc aagattctga aagtacagac aagcagcaaa tacgaaattc 63600 atatttttga tcacaaggcc acagacctct agtaagatgt acgttaactg aggttcgtgt 63660 cggtagtacc aaacgttcat gtaactctca caatgcgaat tgacctgctt tgagctcttc 63720 tatatactaa ctggtattct ggcgaaacaa agctgtggta gtattatgtc atgagtatta 63780 atagtagtaa gaaaatcagg gataatgttt caacggttta cttatttaga ggtagtgtga 63840 ggtactttta cgtgaactag caaactaaca tctcttttaa attattactc ttacaataag 63900 ttccgtgacg acatgattat ctcaacctga tatacaattg atgttcttat attgaaaagt 63960 tattaagcga gatatgtatt taagcatagt agacacatca caagttgcgg atatcattaa 64020 gacaatttat aaatagttcc attgtggaat cttataatat aacctgatag cgttatattg 64080 gtgaagatgg ctaattaccc tctcaccggc gctattagct tccataccaa ttggcgccat 64140 tcattaagat ccgttgttcc ttacgaaaaa aaaatttata agaaatcctg taacatgcgg 64200 atttagtagt ttttattaac aacattactg gagcaaacta aaacatttaa agacagaatt 64260 ttggcagagg caattaatat catatgatgt gaaattaggg tctatattgt aataatttga 64320 taaattaaat tagataaggg gtttggaaga taatttagca cagttagtta taggcggaag 64380 atttgccttc aaaaagcgat tattgttatt tgtcttaagt tacagcgaaa acagcagtcg 64440 ttaaaacaaa aatgatatgt ccaatattac atctaagcaa ggcaatttcg caacaggtta 64500 aggtattttt tatagaaaaa ttaaaggtcc agttcaaaca ctttcatttg tgtagctcgt 64560 ttatgcatat ggtatgtaaa ctagtgcgtc ggtgtatgga gatcttcctt cttgcgtatg 64620 attgcatgga ctgcgatcac gttttgtaag ttcattgaga ctgttacgct taactgctcg 64680 aaattcgaga aaatatacga ctgttcgtaa gagcgctttg atcgaacacg atcataacag 64740 agcacacatg ataatcatca cttttttagt ccctattaca aagcagccaa gtgaatatat 64800 gtctatcact cactatgaat ttgcatatga tcgttttatt gtagaaataa tttaataaat 64860 agaatgttaa tgaagggtct actgtactta tacaattggg ctacatgtta actacaagac 64920 tatatcctat tattgactta gtgtatacat aatatggttt catatatgta gtgttcaagg 64980 gctatagcaa atctgatgat aaatgattag ggtaacacct tagaataata tactagccta 65040 tcggaacaca agcacttctg ccgtataatc cgagtgtggc cacgataatg gaaggtatgg 65100 tcgaccgttg ttgctaatag taggcgataa cctatccttt tttcataaat tttctttaga 65160 acattgttca cataatatat caggattaat tgatgtaatg agctcgcttg attttgaaaa 65220 cttgtgtttt taattcgtct ccgattaata cactatacta aattttaaag ccagatatat 65280 caatattaaa ctatttaatt taacctaatc ctcaaacgtt ctattaaacc gtgtcaatca 65340 atatctgctt cctaaatgaa actttttcgc caaaatcaat agttagaatc taaagtcgct 65400 ttcgtcctta gctatattga tttctttata caggtagtaa cgtagattca ttccgtaaaa 65460 cggtttttca tttggatata aaacatttct ttgatctcca ggagaaatat gagaaactaa 65520 acacgtcgag taaatacgta tactgctcat ttgtccacgc acccacatac ctctggaagg 65580 aaaaacacat actaacctac ctgacgctag tgtaaaatat ccaagagacc ctgacagtgc 65640 gatttaacga gcctttaact ctttcattag ctgataagca ttattgcgaa actagcttga 65700 gcttgtattg tccagtgtgt agtattagaa gtgaaaaaat cagggttaat aactcgtggc 65760 gtcatttaca tactgttagc cagtgacagg taaacgtata ctagcataac gatatcttaa 65820 ttaaattatt tatcttacac attcttccta gatgacatga atatgctggt gcgatgtata 65880 attgaacttc tgatatagga taagaactgg aacacatatg tattatacta atgcatgtac 65940 atcctaagtt ttcaatatcg tttagagtac aatttacaaa tccctttccg gaatcataat 66000 ataagatcgg atagccttgt gttcgtggaa agggcacatt agggtcacag tggtggtatt 66060 acttatcaaa ctagctagca ataacgataa tcatgtacta ctgaacagga aaattgtatt 66120 taaaagaaga cttataacta atgttttaca tagtcctaat tgattacatt actcggggga 66180 actaaaaccc atgaccacta aaataaagct gagtctaatt atgtgatttg atttaaaatt 66240 tcgatctata taattataat ttcgagagat aaattggatt agctatttgc tagatgattt 66300 ggcacagaat gacacagacg aagaacttac tatgaaatag cggtttcagt tgtcaatgat 66360 ggatttcagc gaaactagga aacgatgtag cttaagaaat atctctctca ttgcatttag 66420 ataaagcatc ttgctaaaaa gagaaccagt actttgtaaa gtaactagag gttctcttta 66480 tactctgtga tttgtccagt tcttaaaagc aaacgaggag taatcaggtg cgtgggtaaa 66540 agcagacctt catttttaag tatgattaga tggactacga ttacatttta tgggatctct 66600 gcgactgtca cgctaaaaca ctcggaaaat gacaatccaa tccacaacta gttataaccc 66660 actgatcgaa atggagcata acgggtcaca catcataaat ttcactattt tagtccgaat 66720 ttttcagcat tgcggtagat gtgtaacaac cgaccactga ccatctacat attattatat 66780 tccataagat ttaacttaat atatagaaaa cgcaagtagg atctactgta cttatatcac 66840 catgctacat attaagatgt aagctaaatc caattcttgt ccttgagcat atatatcaaa 66900 aatacgaaca ttttggattc aaaagttata gcaaatccca ccctaaacgt ttggggaatg 66960 gttttagtat tatattctag cctattagaa cagaagcacc attcccatgt aaacccagcg 67020 tcttcatcac attgaatact ttcatcgatt aatattgttt ttaatatatg atgactcgac 67080 cttctaacat aaattttcta ctgattattt ataacaatat gtaccaggat taaccaaggt 67140 actgagcgcc catggttttg ggtactggtg attttatttc gactcaaatt tgcgcactaa 67200 agtaaatttt aaagttagac atacagatat tataggtctc tatatgacgt aaacgataat 67260 caaatagcta aaccgtatgt tactgagtct gcttcttgag tgatacttta gccccttagt 67320 ctacggttac tagcaaaact agttttgttc tttcgataca ccgaattgtt tatgaagaga 67380 gtaacataaa tctattttgt agaacgactt tattctaggt catgaaacat ttcgttcatc 67440 tctaagaata ttacaagaca tcaagtagcc caagattttt cgtttgctcc tgattaatcc 67500 acgcacccat tttcggatcg atgtaaaaat tcattttagt ctatttgata ctattcaaat 67560 ataccctagg cacgcagtca gacgcattat gagactcttt tactgttagg ttaggtgttg 67620 ttcgatactg ggtgtctgcc aatacctcat attgcccaca gtgtcgaatt taaatcggtc 67680 aaattaggtc ctcaaagttg taacgccatc tccacattgt tgggtggtga ccggtaggtg 67740 tataataata taaggtattg aaaattgaat taaatatact ttgcgcccgt tctacatgaa 67800 ataaattcag tcgaacgatg caagaatcta cattcgaacc aagttatgag cgcgaactcc 67860 tatttaaagt ttttatcgtt caaaaacctg agagtttaac aacgttttac gtgggacatg 67920 caaacccctt accaatgtcg aaatatagga atgaacaaat ctgtcttcgt gctaagggta 67980 caattaggac gctgaagtaa tgtaacttat atgcaaggca aattataaca aaaattaaat 68040 tccagtaagg tggaagattg tgatttaaag atgactaata aatattgtta tacatcctcc 68100 taatcggttc tacggtttac gagcaccaaa atccaagacc tctaaaataa agctaagttt 68160 agacgcgtga ttatacttaa gatatcaaac tgttcgtcta ttatatccag agatattctg 68220 tatctcttat taatttatcg atctcgtata caatgactca tttatagaac tcagtataga 68280 atctaaggat cgggtgccaa tgatccttcc gatcagaaca tgaaagctat gtggtctgac 68340 gattacttct ctcaatgtct ttagaaaaaa aatcccgctg caataggatc cagtacttag 68400 taaagcaggt tgtgaatctt ataatgtttt gaagatcatt ggattttaaa aaccaaacgt 68460 ggactgatta gaaacgtaag ctaaaatcta gccacatttc taactagttt cagataaatt 68520 ataataggtt tatatggtat gcgtacccca ttctgcataa tactttaaga gaaagacaat 68580 ccaatgttga tcaagctttg aattacaaac gattatgatg taataggggt gttatggctt 68640 atattaagcc agaaattttt aggagtatca acattgccct tcggctctaa taactcacta 68700 gtggtcatcg acatgataat atattcgata atttttaact aattttattt gtatggcggg 68760 cataatatat ttagtttgag tctgcttgca acgatgttag acataagctt ggttcaatac 68820 tcgcgcttaa agaataattt caaaaacagc gagtgtttgg tgtcccaaat agttactaaa 68880 tgcaccctgt acgctagcat aacggttact actttatatc cattcttcaa tagacagaaa 68940 gaagattctc tcgttaattt tccaacttct ttacattgta tatacgttcc ctttaatttc 69000 ctattagaat taaggacatt ccatcatcta acactaaatt tctactgatt tattataaca 69060 acatgtaggt ggattaggca agacccgaaa tgctggttgt ctttggttct ggatattttt 69120 tctcgaacta tatctgcgcg ttaatatgaa ttctatagat ctacaagcag aaaagataag 69180 tcggtttaag acaatgggaa aagttagata cctagagtat taattattga gtaaatatct 69240 tctcatatat gatagattcc taccccccgg ttgctaggaa ggttagtctt gtagtttcca 69300 tagacctgac tgctaaagaa gagagtttct gatatctttt ttttagggcg acgtaatcct 69360 agatggtgat tcaaatggac caacatttag aacattacaa ttcttctagt tacctagtat 69420 ttttattttg cacctgggaa atcattgctt ttgattctag atctgtgtaa agattgatca 69480 gacttgaatt aaatttgtcc aaattatcca tgtgcatgag acaacatata ttatgaaatt 69540 cacttttcaa aaggtcacaa ctagttggag acttattgtt tgctaatatt acattatcct 69600 cacattagcg attgtagttg gctctctaaa aatcgtcata gtaccaatgg ggagccgaga 69660 ttattgagtg attaccagta gctaaactat ttaataagct attaaagatt gatcaaagta 69720 aacataccgc ctgaaatata taaatcatag tcagaccaac attgctacaa ttcgtattcg 69780 caccaacttt tgaccgcgta tttcccattt aagcttttgt tgctcacaaa acacttggta 69840 tacctatcaa tctcatggga catgcgatcg tactgtgtac gaaggtattt cggtaagaag 69900 ttatgtcact tttatctaag taagcaaata aaaagataaa aaaatgcaat atatatgcaa 69960 gggaaattaa tggacgatct taattcctgt aaggtaacac attgtgactc aaaaatcatt 70020 aaataatata attgtacatc tggctaatcc gttctgggct tttcaaccaa cagtaacgga 70080 gacttattaa aaagattttg atacggacgc gcaactattg ttaggtaact ttgaagtccg 70140 tcttttcgaa tcagccaaat tcacttacct ttttgaatct tcggatgtca taattaatag 70200 ctcgtttata gaagagtata cactatctaa gggtggaggg ccaagaatcc ttccaattac 70260 tacattaaag atatctgcac tgacgttttc tcttctcaag gacaaaagta aaaaaatgcc 70320 attgcactag gatttaccgc aaagattacc tcgttgtata tattgcagtc ttaagaacat 70380 caatggatca taaaaataaa acatgcatcc tttactatta aaatctaagt tctagacaaa 70440 ttaccaatta gtctgaactt acattaaaga gatttaatag gtacacgatc tctgtcgtat 70500 aaattacttt aactgaaatg ttttccagta ttaatcaatc cctgaatagc atacaattat 70560 aatgagacta gactataaac gttagcaacg acagagagat tattagcagc atcatagtta 70620 cctctccgct ctagtatcac acaaatggtc gtcgatcaga tagattggtc gataatctcc 70680 atgaactatc gtctgtatgg cggactttat aaatttagta tcagtctggt ttaaatgatg 70740 ttgagcatta gtgtgcttaa aaaccgtcac ataaagcgta tattcgaaaa caacgagtgt 70800 cttgtgaacc atatgcataa ttagagtact ctgtagacta gtatgacaca tggctccaca 70860 aagctattta tcgatacaat aaccattaga tactgtgggc tttgacgttg ctaaccagcc 70920 aaccatctat gtttttgaaa taaagaactc atatctttct ttaatgttct acaaatatgt 70980 ctacatcctt ttttctaggt catgaaacat ttcgttcatc tctaagaata ttacgagaca 71040 tcaaacaggt caagattttt cgtttgctcc tgattagtcc acgcacccat tttcgcttcg 71100 atgtaaaaat tcattttagt ctatttgata ctaatcaaat ataccctagg cacgcagtca 71160 gaggcattat gtgactcttt tactgttagg ttaggtgttg ttcgatactg ggtgtctgcc 71220 attacctcgt attgcccaca gtgtcgtatt taaaacggtc aaattaggct ctgaaagttg 71280 taacgccatc tgcacattgt tggctggtga ccggtagatg tataataata taaggtattg 71340 aaaattgaat taaatatact ttgcgcccgt cctacatgaa ataaatttag tggaacgatg 71400 caaaaatcta cattcgaacc aagttatgaa caggaactcg tatttaaagt ttttatcgtt 71460 caaaaaccta agtcttcaat aacgttttgc gtgggacatg caaacccctt accaatatcg 71520 taatatagga atggacaaac ttgtcttcgt ggtaagggta caattgggac gctgaagtaa 71580 tgtaacttat gaggaaggct aattataaca aaaattaaat tctagtgagg tggaagattg 71640 tgttttaaag atgactaata aatattgtta tacatgctcc taatcggttc tacggcttgc 71700 gggcaccaaa atccaagacc tctaaaataa agctgagttt agacgcgtga ttacacttaa 71760 gatatcaaac tgttcgtcta ttatatccag agatattctg tatctcctat taatttatcg 71820 atttcgcata caatgactca gacatagaac tcactataga atctgaggat caggtgccaa 71880 tgatccttcc gatcaaaaca tgaaagctat gtggtctgac gaatacttct ctcattgttt 71940 ttagataaaa aatctcgctg aaataggatc cagtacttag taaagcaagt tgagaatctt 72000 ataatgtttt gaagatcatt ggattttaaa aaccaaacgt ggactaatta gatgcgtgag 72060 caaaaatcta gctacatttc taactagttt cagataaatt atgataagtt tatatggaat 72120 gcgtacgcca ttctgcataa tactttgaga gaatgacaat ccaatgtaga tcaagctttg 72180 aattacaaac gattatgaag taatacgggt gttacggctt atatttagcc agaatttttt 72240 aggagtatca acattgccct tggggtgtaa taactcacca gtggccatcc acatgataat 72300 atattccata acttttaact aattttattt gtaaggcggg caaaatatat tttgtttgag 72360 tctgcttgct acgatcttag acataagctt ggttcaatac tcgcgcttaa agaataattt 72420 caaaaacagc gagtatttgg tgtcccaaat agttacaaaa tgcaccctgt acgctagcac 72480 aacggttact actttatatc cattcttgaa tagacagaaa caggattccc tcgttaattt 72540 tccaacttct ttacattgta tatacgttcc ctttaatttc ctattagaat taaggacatt 72600 ccatcatcta acactaaatt tctactgatt ttttataaca acatgtagga ggattaggca 72660 agatgcgaaa tgctggttgt ctttggttct ggatattttt tctcgaatta aatctgcgcg 72720 ttaatatgaa ttctatagat tgacaagcag aaaagatagg tctgtttaag acaatgggaa 72780 aagttagata cctagagtat aaattattga gtaaatatct tgactcatat gatagattcc 72840 tagcccccgg ttgctaggaa ggttagtctt gtagtttcca tagaccagac tgctaaagaa 72900 gagagtttct gatatctttt ttttagggcg acgtaatcct agatggtgat tcaaatggac 72960 caacatttag aacattacaa atcttctagt tacctaatat ttttattttg cacctgggaa 73020 atcattgctt tcgattttag atctgtgtaa agattgatca aactcgaatt aaatttatcc 73080 aaattatcca tgtgcatggg ataacatgta ttatgaaatt cacttttcga taggttacaa 73140 ctagttggag acttattgtt tgctaatact acattatcct cacattagcg aatgtagttg 73200 gctctctaaa aatcgtcata gtagtaatgg gaagccgaga ttattgagtg atcaccagta 73260 gctgtactat tttataagct attaaaaatt gattaaaata aacataccgc ctgtaatata 73320 taaatcatag tcagaccaac attgctacaa tctgtattcg caccaactta tgaccgcgta 73380 tttcccattt aagcttttgt cgctcacaaa acactaggtt tacctatcat tctcatggga 73440 catgcgatcg tactgcgtac gaagatatat cggtaagaag ttatctgtct tttttctaag 73500 tgagcaaata aaaggataaa aaaatgcaat atatatgcaa gggaaattaa tggacgatct 73560 taattcctgt aaggtaacac attgtgactc aaaaatcact aaataatata gttgtacatc 73620 tggctaatcc gttctgggct ttacaaccaa cagtaacgaa gacttattaa aaagatcttg 73680 atacggacgc gcaactatag ttaggttact ttgaagtccg tcttttcaat tcagccaaat 73740 tcacttacct ttttcaatct ttggatctca taattaatag ctcgtttata gaagagtata 73800 tactatctaa gggtggaggg ccaagaatcc ttccaattac tacattaaag atatctggac 73860 tgacgttttc tcttctcaag gactaaagaa aaaaaatccc attgcattag gatttaccac 73920 aaagattacc tggttgtata tattgcagtc ttaagaacat caatggatca taaaaataaa 73980 acgtgcatcc tttagtaata aaatctaagt tctagacaaa ttaccaatta gtctgaactt 74040 agattaaaga ggtttaatag gtacacgatc tctgtcgtat atattacttt aactgaaatg 74100 ttttccagta ttaatcaatc cctgaataac atacgattat aatgaaacta gactataaac 74160 gttaacaacg accgagagat tattagcagc atcatagtta cctctcggct ctagtatcac 74220 acaaatggtc gtcgattaga tagattgctc gataatttcc atgtactttc atttgtatgg 74280 cggactttat aaatttagta tcagtctggt ttaaacgatg ttgagcataa gtgtgcttaa 74340 aaactgtcac ataaagggta aattcgaaaa caacgagtgt cttgtgaacc atatgcataa 74400 ttagagtact ctgtaggcta gcatgacaca tgcctccaca aagctattta tcgatacaat 74460 ataaatagat atattcgcct atttttctgt ttatatccgt tatatttacg ttgtctttaa 74520 ttacctgcca gaattaaaga cattccatag tgtaggactg ggttttcagt atttcattat 74580 ataaacttga tgaccagttt ggctagtccc gacaagttgg ttgtgaatgc cctcgaataa 74640 ttcttctaaa acaatgcctg ggtgttgaca gcaatccatt gaaacttcag gcagaaaagc 74700 ctagccggtt taactgagtg gaaaaactta gaagcgttct ataatgatta tcgaggaaat 74760 atattctcat ttgtgacaga tttccacctc cggattctta gaaaggttat aggagtaatt 74820 actattgacg tgagtgcagt ggagaagaaa tcatgttttc attttatgac ggtaacgtga 74880 tcctgaatgg cgtttataat ggagtaatat atttaacgtc ggaattcttg tagttatcta 74940 gtatttatat tttgaatata ggagataata attttacatt caagaattgt ttcatgctta 75000 atcagtcttg aatgtaattt ctctaaatca tccatgtgcc aaagacagca tatttaatca 75060 aataaagttt agaaaaggtc ataattagtt acggagttat cctatgttaa tattactctg 75120 atctgatatt tgcaatcgct gctgtctctc caacattcgc cgtaatatca atagagaggc 75180 aagatcttac tatgttttcc aagggcaagt ctaactatcc agtcattaga gggactcgat 75240 agcagacgta ctgcctgaaa tatttacatg ttagtgagat taaatttact accagtcgta 75300 aacacacgat tttttagcag tttatttcgc atataagctt ttgttgctta caggacactt 75360 ggtattcgta atgatctcat gaggcgtctg ttcatactgt attccgaggc gctccgataa 75420 atagctatga tatacttatc tatattagtc gataaaaaga caaatatagc gattaaaaat 75480 gcaacaaata ttaatgaacg gtctcaaata gtgaaagata tcgctccttc ggccatctct 75540 caaaaagcca tatattcgaa ttactgatga agcccatcag ggctgttcta tctagtctta 75600 taggagttta ttttgaggtt attgttaccg accgacaacc gtcgtgtcgt aactttgaag 75660 tccgtgtttc cggatcgtct gaattggttc acctttttga atcttcgtag gattatacta 75720 atagttgctt tttgtaaaag taaacactcc ctataggtag tggtctatga gtttttgcat 75780 tatccttatt attgataatt ctactcatgt cacgtctact atagtacaaa tttattatac 75840 caccatagca ctagatctta tggagaatac tctctcatta cataaattgg agccacaggg 75900 acatgaatag atgataaata tagagattat gtcctcaatt gtaaaattgt gagttcttaa 75960 caaagtaggt attagtcaga acttacatta atgacattag gtatttgcac agtttcagtg 76020 atatagatta gttcatttca aatcttttta cgtattagtt aatgcctcaa tagagtagaa 76080 ttataataac actatattat aaacgttagc gacgacagag aggttgaaag cggcattcta 76140 gtaatctgtc cgttcgagaa caatataaaa tgttcctgta cagattgata ggttagtatt 76200 ctccctaagc tgtcctctcc ataacggact ttatagtagt acaatcgctt taatttaata 76260 catggtcagc atttttgtgc caaaaaatcg tcaaattaaa cgcatatcgg aatgcaatga 76320 atgtgctatg gaccttaagc attactagaa tagttcgcag acaactttgt cataaggctc 76380 cgcgacgcaa tttaacgata ctatatcaat aaaaataatc agctatatct ctgttaatat 76440 cgccaattct tacgttgatc aatattgctt gccggagctt atcatctttt atagggagga 76500 ggcccgtaga ggactttacg gtatataagc ccaataactt ctgccaatgg tgccgtcaaa 76560 atagatcgga gtttccagat ataaagctcc aataagaatc gctgacacaa cgcagcacag 76620 ggtcgaaact tacgcaatag tggcctaaca gactgaacca agtggaaaat ctaaggagta 76680 acccaatatg attgactgtg aataagaaat tcattcctga ggaataaccg tcaccactta 76740 ctctaaaccc taataggaat aataattatt aaaatgagga cagcgtactt aatgtcatgc 76800 tcgaataaaa tggtcctata gtgaactaga ttacctccta tgagaaagtt aagaacttaa 76860 cctggatgtc tctactcaca tgatctacct gcatgtgtaa tacacgagtc aacatattaa 76920 cacacaaaag ttctttcttc catatgcagt tatgtaagtc atagctgtat tccataaacc 76980 tatcaaagtc actatatctg gttatctaaa gtctagagaa atggatagtc atttatggta 77040 atatctcaat taagtataat tgtgataaaa tgtgtgtagt ggctcctatt ttcccgactt 77100 tcactgtgag atgattaaac aagtacacac ttgttattta ttataagctc atgtgcaact 77160 accctatcac gagaccgatc cgacaggtga agcatatata agaaactcat cacgtctgcg 77220 taattaaatt atacaccagt cgtaaaatcg tactttacta ccagtttaac atgcgtatgg 77280 tgcaacccta catacacgat acctgaaaat cgtaatggac ttagtaacag atagctaaaa 77340 cggtattccg aagcggtgca ttaattcact aaggtatgga tgttctcatt acacggctta 77400 gatacaatta tagtggcaag gaacataatt agttattacc atcggcgacg aatagtagtt 77460 tatttccttc ctgtgggctc ctcctgaaat gccacttata cggcatattg aagccgatta 77520 gtacaataga tttattcaac ctcaaaggtc tatattcccg gcttactctt agctgatatg 77580 tcgcgtcaag tctcgggatt gagtgcatta gcaccggatt gtctggctta gttcaactat 77640 tggatagctc atcgggttat actaactgac acttattctt taaataacgt tcccttatta 77700 ggactagtaa ctgagatttg aaactatcct tatttctaat catgttaact ctttcgcatg 77760 aataacagta cgagcttact ttacgagata atctcttatt caagtgcagg atactcttcc 77820 aattcttgaa gaggacgtag agtgttgaat gtattagaag ggcgtacgca ttatctgccc 77880 acttgtatat ttatgtgttt tcaaggaagg aagtatgcat caattcgttc agtatcgaca 77940 taaggtattt ggaaagtttt agtgacatag tccaatagat tcttgatctc cttaccgatc 78000 agtaaatacc attatacagt caattcagat ctacgctatt taacacataa cgccgaggat 78060 aaaagggctg aaattgacag actacaatat tgtttatgtg tcatcaatta acaataaacg 78120 aatacatgtt gatgcgatag agctctggcc aggccgtcca cttcgtatat tttctttgag 78180 tagtgtagat gtattaatat agcatgtagc cagattctta gcatgaagcg aagattaaaa 78240 tgtacgcatg ccacgttcac atatatgtgc tttagatttt tagtgttacc tgaaacatag 78300 tccatagatt ttcctttaag actacggcac gaagttaagt gatttcacac ctacaagaga 78360 aatatgccca aactatgata ataacaccgt tcgttgaatt aatcaataat gatagcggtt 78420 gcatattatc aaatgaagga gccacacccg gggagcagtt tacggtcgat atctcgtaca 78480 acttcggcta atcatgttat ctaaaaaagt tgaggtttgc agttgtcagg gcgaaatcag 78540 attcgactat atagcccagt tcagagccct aactcacgta ttcgtgacct tagagagcga 78600 gtcaagctaa ttactcattg actagctcaa tatgaggcag tgtgaataag aaatttattg 78660 caaggcaata atcctaatta ttgatagcaa actttgataa ggttgaagaa tagcacaatt 78720 cagaaagcgt tctttctgtc atactcgaat gaaatgcttt attaaagaaa aggttgatgt 78780 cctacgagat gattaagaac ttcttctgca tctcacaacc tacttaatct tcggggatga 78840 ccaaaacagt cttcgatgtc ttaatgactt atcgtccatg aatcatctat tacttccatt 78900 tcgcctgact gttcgttcaa atctgcacca ctaactacaa atattagttt aatttttatt 78960 atcctctaaa tgcgataaat tgtgtattgc ggctcctatt ttcccgactt taactgtcag 79020 atgttataac aagtacacag tagttaattg ttatttgctt atgtgcaact acgctatcac 79080 gagaccgatc cggcaggtga agcatataaa agaaactcat cacatctaca taattatatc 79140 gtacatcggt ctaagaatcg tactttgctt ctaattttac atgcgtacgg tgcaactgta 79200 tatacacgaa atctaaaaat cacaatggac tttgtatcag gtatctaaaa ggaaattctg 79260 atgccgtgct ttaattcact aaagtgtgga tgttctcttt atacggcttt gatactatta 79320 ttgtggcaag caacttaatt agttattacc atcgccaacg tataatagtt tacttcctcg 79380 gtgtgggccc ctcctcaaat gccagctata gagcatgttg aagccgatta gtacaataga 79440 ttttttcaac cccaaacgtc aacagtcccg ctttagtcta agctgatatg tcgggtcaag 79500 tctcgggatt gagtgcatta gcactggaat ctctcgctca gttcaattaa tggataactc 79560 atcgagttat actccgtcac acttattctt taaataacgt tccgttatta ggattaataa 79620 ctatcgtttg aaactattcc aatttcttat cgtgttaagt ctttcgcaag aaagacagta 79680 tgagcttact ttacgaaata atttcttttt caactgcagg atactctact aattcttgaa 79740 gaagacgtag agtgttggat gaattagaag cccctaccca ttttctatcg acttgtatat 79800 ttatctctat tcaaagatgg aagtttgcat caatttgttc agtatcgata tagggtgttt 79860 cgaaagtatt agtgacatag ccctatagat tcttcattac caaaccgaac actagttagt 79920 attaaacagt caattctgtt ctacgccatt taacacataa cgctcaagat aacagagctg 79980 aatttgagag actacaatat tgtttatgtg tcaccaatta acagttaacg aatacatgac 80040 gatgcggaac acctcgggcc aggccgcgca cttcctatat ttactttgag cagtgtaggt 80100 gtaataatat agcatgtagc ctcatgctta acttgaaccg gagataaaaa tctatgcacg 80160 cgaccttcac atttacaagg tttagatttt taatgctacc caaaacaaag tccataggtt 80220 tttcattaag actacggcgc aaagttaagt gatttcactc ctacaacaga aatatgccca 80280 aactatgaaa acaacatggt tcgtagaaat aatcaatcgt gatagaggtt gcatattgtc 80340 aaatcaaaga gccacactcg gggagcagtt gacggacgtc atcacgtgca atttcggcta 80400 atcttataat ctataaatgt tgaggtttgc agttgtcagg gcgagatcaa attcaagtat 80460 atagtcctgt ccagagcctt aattcacata ttcgcgacgc ttgaaagcgc gtcatgctta 80520 tcactgattc actagctcaa tgtgtggcaa tctaagtagg aagtctgttg caaggctaaa 80580 aaactaaata ttgatagcta ccttagttaa ggtagaagaa ttgcacaatt cagtaggcgt 80640 tttttctgct atactcgaac ggaatgcttt attaaagtaa agcttgatgt cccacgagat 80700 gaacgagaac ttctataaca tctcacaacc tactaaattt tgggggatgg gtaaaagtta 80760 gctgaacata caaataggaa tttgattgta cccttagacg aaactaaaca aatcatagta 80820 aatccgcaca aagtttacat attaattcat acggcgtttc aaagaggcaa tggcttgact 80880 tgtggtgaat tacagtttgt gagtaaccac aagatgcggt aaatactgta atccgagttt 80940 aattgactcg acttaaagat tctgatatta taatgaaaac acaatggttt attgtcaatt 81000 acttatgcac tgttacatct tgtggtcctc ggtccagcct gcgtaggata taaatgaagc 81060 acgtctcatc cagattgatc tatcgtacct ccaaatacga attcaacttg gcgtctatat 81120 ccagaaatga gccctagaag tgatagactt ccaaatcttt aaactaccat gggatatgtt 81180 tcacgtatcc aaaaagtaat tataattctg cgtttccatt cacttaagtg aggatgtagc 81240 ctttatattg gtttcatagt tttgttatac caagcatgta ttttacctag cactattttc 81300 aacctaaaac tgttttgttt ctcgatgtca aacccttgtc agctacctgc ggtggtgcac 81360 gaaaaaaccg attagaatat taaatattta caactccaaa ttttaagagc cctgctctag 81420 tttaaattca tatatcagta taggcttcgc aatttagtga ataagcggta tgaattttcg 81480 cggagtatga aaagtgacta agtaatcgag atacataccg ttagatagat gcttcagact 81540 gcattctgaa tttttgattt ataaccatcg aaggaatcaa tttcatgttc ctaacgtgtt 81600 aagtcatcag taaaaaagac cataagggct ccccttttga aataatactg tttcaaacta 81660 taggctgccc tacttggtgt tcaagatact atagagtctt cgatgattta aagcccccaa 81720 cccatgttca atcaacttct atgtttaacc ttaaactaac acaggaacca gctaagattt 81780 gtttagcatc atttgggcac gttacatata taaaataaag tatgccgcag agtttctccc 81840 ttaccgaact gtagtcgacc taaagtctaa tactcattgg tgttgtatca cttttgtgac 81900 attaggtatg atttaactct tgaaaatttt tacgactatg ataaaagatt agagctacca 81960 aataactgtt aataatcatg tcactatgta gatcgccaag aatcagctgg gatgaatgca 82020 ttatttacat cgtgcaaact aggtctgagt aggtaccatc gtagtttatg cctaagttga 82080 accgcaaata taggtctttt gccgggatct ccactatctg taaattgtga aacctgttgg 82140 taacctatac aaaatacata gctatatcat tagtaataaa acgtaaacga aagatcattc 82200 actccttctt cggaaagata accaaagaat taggagaata tggtgcgcac atagaaagga 82260 tcgggaaata actttgacct tcacagaaca ataagttaca atttgggaac agtcggataa 82320 cgccactacg tgcttatttg gtttacctta aattttataa ttgatgagga ttaaaattca 82380 cgggagaaga tctaacataa gtatatagtt atatctgaag cgtttgaaca cttactccct 82440 ttacttaatt aggccttatt gttttccctg actcataagt tctatgattg gcaatctatc 82500 tacaaggaga cacgtatcat ttaaaaacaa aaaattggta acttccttag ttaaagtgct 82560 gggaatgctc aaatcagtag tcttcttctc tggaaatcgc gaggggggaa ttttatcatg 82620 tcgaagtttg ttattggact cattcaacca taggctccat gatattaaaa gagctagtaa 82680 actacggggg taggatacaa atttcttgat aatagaaatc ggaattcgaa tgtgtccttg 82740 gtcgactcag atccaatcca agtgaaccca acgaatgtat atattttatt acatatggcg 82800 tcttaaacag ggaatccctt aacatcaact ggttttcaga taataagtaa ccgcaacata 82860 gtgaaaacac agttatcgat actagttaga gaacctttaa taatgctaca accattttct 82920 aatcttgatg atttgttgac aattattagt tcagcgagcc atctaacggt tcttagtcaa 82980 ctctacttat gcaataaatc ttgcatgttt gacgctaact caaccatggt agcatcaaat 83040 acggatacaa ttaggggtta atatgcagaa aatgggctta gggatgatag acactttaca 83100 aattgcacaa gcaatcgaaa tgttttatgt atcgatatag tatgcatttt tatagaattg 83160 ccttctagca tgacagaaac aagcctatct attcgtttct ttatcctcct ataccacgtg 83220 tgtacctttc ccggccattc attaagaccg gaagtatctt gttatacaat attaaacctt 83280 taacagccta tcgcgctgat acacgaatta accaaatgaa gtttaaaaaa ttaactactg 83340 ttatttttaa gtggcttctt caagattgca ttcatatatc aataatgact tactaaggtt 83400 ctaaatgaga gaattcaatt aatctggata aacaaaaggg tctgaagatt cataatacca 83460 accattaaat agacgttcct ctgtgtatag taaatcttta ttttttaacc tttgaagaaa 83520 tcgacaacac gacccttgcg agtatagtta tgaaaagaag agatcttcat ctttgccccc 83580 ttataatagt acagcttcaa acaatattct cagtaagatg gtatgtgtgg tactaagact 83640 ttcatgacta tttgatgtcc ttattctatg tataaagagt aataatctta agtctggagt 83700 ttactcagca accagttgag tctaggcaca gctttcttgg gttgcttata tattgaaaat 83760 aatgtactca acaggatcag tctcataagg aattgtgatt gaccaaaagt ccgctatgca 83820 ttgacgtcgt ctcagtttag tacctataac tatcatcatt cttttggtgg ctattacaat 83880 gttggtaaaa gaataaaact acaacacatc tgttaataat caactcgctc gattgattgt 83940 caagaatgtt tagtgatgag tatgttattt aaggcactcg agttgtgatt gagttggatc 84000 cattgtagtt tacggctata taagtcctca ataatacgct ctatacgcaa attcctatta 84060 tctgtgaaat gtttaacgcg tacgctaact ctataaaata cttaactata ttatacgtta 84120 gaatatctta acggacaatc gcattgtatt tatttgtata gattaatgaa aaagtaggtg 84180 cgaatattgt aggtatggta aaggccggta agtaattcta gccttcatgg aacgttatgt 84240 tttaatttga aatttaaggg atagtgcgtc taaatcgtta ataaaattag ttgaaatcac 84300 ctagttagaa tctgtaaaaa ttcaccataa aagttccacc gtaaattaat gattactact 84360 gtataattcc aaaatttact ctcttaagtt aattaaacca atctgtttcc ctaggcttct 84420 ataaaatagc gtcggtaatt ttcccgcatg gagacagatg ttattcggaa ataaaaattt 84480 acaaacttct ttggcacttc tgctgacaac agttttgttt atactattct tgtcatgatg 84540 tagatacgac gcgttattat catgtcgaag ttcataataa gtgtcatttt accatacaga 84600 ccatgattct gaaagtactg acaagctgca aatatgaaat tcatatttct catcacaagg 84660 ctacagacct ctaataagat gtacgttaac tgagattcgt gtcggtagaa ccgaacgatc 84720 atgtaacttt cactatatga gttgacctgc tttgagcttt cctttatact aactggtatt 84780 ctggcgaaac aaagctgtag cagaatcatg tcatgagtat taatagtagt aagaaaatca 84840 cggataatgt tacaaccgtt ttcttattta gaggtagtgt gaagaacttt tacatgaacc 84900 agcaaactaa catctctttc aaattactac tcatacaata agttccgtga cgacatgact 84960 atctcaacct gatttacaat taatgctctt atattgagaa gttattaagc gagatatgca 85020 tttaaggata atagacacat cacaaattgc ggataccatt gagacatttt ataaatagat 85080 gcaatatgga atcttataga attgcctgat agcgttatat tgatgaacat gactaattac 85140 cctttcaccc acgctattag cttccatacc atttgcggcc attcattaag atccgttgtt 85200 ccttacaaaa aaaaaattaa taagaaattc cgtaacatgc agatttagta gttattataa 85260 acaactttac tggaacaaac taaaacattt ataaacagaa ttttgatagt ggcaattaat 85320 ttctaatgat gtgatattaa ggtctaaaat gtaagaattc gataaattag attagacaag 85380 gggatccgaa gatattttag cgcagttagt tataggggga acctctgcct acaaaaagcg 85440 tttattgtta attgtttaaa gatacagtga aaacgacagt cgttaaaaca aaaatgatat 85500 gtacaatact acatctaagc agcgcaataa cgcaacagct tgaagtattt ttcacagaaa 85560 aatcgtacgt ccagttctaa cactttcata tgtgttcgtc gtttatgctt ttagtatgaa 85620 aactagtgtg ccggtgtctg gagatcatcc ttcttgcata tgactccaag gacagccatc 85680 atggtttgta agttcattga gactgttacg cttaactgga cgaaactcga gaagatatat 85740 gattgatcgt aagaccgctt tgattcgaca ccatcataac acagtactca tgattatcat 85800 cattttttta gtccctatta caaagctgcc aagtgaataa atctctatca cactctatga 85860 aaatgcactt gatcgtttta ttgtagaaaa aatttaataa acagaatgtt aatgaaggca 85920 ctactgtact aatagagttg ggctatatgt taactacaag actataactt attaataact 85980 cggtctatac ataatttggt atcatgtatg tagtgttcaa gggctatagc aattctgata 86040 aaaatttatt aaggtaacac cttagaataa tatactggtc tatcgcaata caaccacttc 86100 taccgtataa tccgagagtg gccacgataa tcgaaggtat ggtcaaccgt tgtaagtaat 86160 tctaggcgat aaggaatcct ttttttataa attttcttta ggacattgtt cacgtaaatt 86220 atcaagatta attgttgtaa tgagctcgtt tgattttgaa aatttgtgtc ttaaattcgt 86280 ctccgattaa tacactatac tatactttaa tcccagatat atcaatatta aactatttaa 86340 tttaatctaa tcctcaaacc ttctattaaa tcgtgtcaat caatatctgc ttcctaaatg 86400 gaagtttttc gccaaaaaca ataaacagaa tttaaagtcg cttttgtcct cagcaatttt 86460 gtttttttta tacaggttat aacgtagatt cattccgtta aagcgttgtc caattggata 86520 aaaaacatct ttttaatctc caggtcaagt ttgtgaaagt aaacacgtcg agcaaatacg 86580 tataccgcac atttgaccac gcagccacat acctctggaa ggaaaaacac atactaacct 86640 acctgacgct agtgcaaaac attcaagaaa ctctgacaat gcgaattaac gagccttaaa 86700 ctcttttatt tgctgataag cattattgcg aaactagctt gagcttgtat tgtctggtgt 86760 gtactattag aagtgaaaaa atcagggtta atgtctcgtc gcctcattta tatacagata 86820 gtcagtgata cctaaacgta tactagcaaa atgacatctt aattaaatta tttatcttac 86880 atattcttcc tagatgacat gaatatgttg gtccgatgta taattgatgt tctgatatag 86940 gataataact gaaacacata tgtattatac caaagtatgt acatcacaag ttcccaatat 87000 cgtttagact actatttact aatcccattg tggaatctta atatatgatc ggatagcctt 87060 gtgttcgtga aaacggcata ttaggctcac agcggtgcta ttaccttcca taccagctag 87120 caacaacgat aatcatccgc tattggatag gaaaatagta tttaaaagaa atcttgtaac 87180 aaatgtttta tatagtccta attaactaca ttactcgagc gaactaaaac ttatgatcac 87240 aaaaataaag ctgagcctaa ttatgtgata tgatttaaaa tttcggtcta tatagttata 87300 atttgatgaa ataaattgga ttagcagttt gctagatgat ttggcacaga tagtcataga 87360 cgaaggactt actatgaaat agcggtttta gttgtcaatg ttggatttca gcgaaagcag 87420 gaatcgatgt agctaaagaa atatgtccat cattgcattt agataaggca tcttgccaaa 87480 aagagaacca atattttgta aagaaactag aggtcctctt tatactctat gatttgtgca 87540 gctcttaaat gcaaacgacg agtaaacagg tgcgtgggtg tatggagacc ttcattttta 87600 tgtatgattg gatggactac gatcacattt tatgggttct ctgcgactgt cacgctaaat 87660 tactcggaaa ttgacaatgt aatcgactac ttgtaataac ccactgatcg aactggagca 87720 taacgggtca cacatcataa ttttcactat tttagtccga attattcagc atcgcagtaa 87780 atgtataaca atcggccact gtccatttac atatcatcgt attccattag aattaattta 87840 atatatagaa tgtgtaagta ggatctactg tacttatatg accatgctac atattaacat 87900 gaaaactata tccaattctt gaccttgtgt atatataata tgaatacgaa catgttggat 87960 tcaaaagtta tagcaaatcc catcttaaat gtttagggaa aggctttagt attatattct 88020 agcctattgg aacacaagca cctttcccat gtaaacccag tgtcttcacc ataatgaata 88080 ctttcatcga tcgttattgc ttttagtaca tgatgactcg tccttttaac ataaattttc 88140 tactgaatat tcattacaaa atgtaccagg attaaccaat gtaatgagcc cccttgattt 88200 tgggtactgg tgattttatt tcgactcaga tttacacact aaagtaaatt ttaaagctag 88260 acatacaaat attaaagctc tctatttaac ctaaacgata aacaatcagc taaaccgtgt 88320 cttactgagt ctgcttcttg aatgatactt taccgccaaa gtcaacagtt actacctaaa 88380 gtcgctttga tcttttgcta caccgaattc tttatgaaga gagtaacgta aatctatttt 88440 gtagaacgat ttttttctag gtcatgaaac atttcgttca tctctaagaa aattatgaga 88500 cattaaacag gtcaagaatt ttcgtttgct cctcattagt ccacgcaccc attttcgttt 88560 cgatgtaaaa attcatatta gtctatctga tgctaatcta atatacccta gacacgctga 88620 cagagccatt atgtgagcct tttactgtta ggttaggtgt tgttcgatac tgggtgacta 88680 gcattacctc gtattgccca gagtgtggta tttaaaatgg tcaaattagg ctctaaaagt 88740 tgtaacgcca tctgcacatt gttggctggt gaccggtaga tgtataataa tataaggtat 88800 tcaaaattga attaaatatg ctttgcgctc atcctacatg agataaatat agtggaacga 88860 tgcataattc tacattcgat tcaggttatg aacaggaact cgtatttaaa gtttttatcg 88920 ttcaaaaacc taagtcttca ataacgtttt gggtgggatt tgcaaacccc ttaccaaaat 88980 cgtaatatag gattggataa acttgtcttc gtggtaaggg tacaattggg acgctgaagt 89040 agtgtaactt atgagaaagg ctaattataa caaaaattaa attctagtga ggtggaagat 89100 tgtattttaa agatgactaa taaatattgt tatacatgct cctaatcggt tctacggctc 89160 gcgggcacca aaacccatga cctctaaaat aaagctgagt ttagacgcgt gatttcattt 89220 aaaatttcaa tctgtttgtc tattatatcc agagatatac tgcatctcct attagtttat 89280 cgatttcgca tacaatgact cagacaaaga actcactatg gaatccgaga atcaggtgcc 89340 aatgatcgtt ccgatcaaaa catgaaagct atgtggcctg acgaatactt ctctcattgt 89400 ttttagataa aagatcttgc tgaaatagga tccagtactt tgtaaagcaa gttgagaatc 89460 ttataatgtt ttgaagatca tcgggttcta aaaaccaaac gtggactaat taggtgcgtg 89520 ggtaaaaatc tagctacatt tctaagtagt ttcagataaa ttatgataag tttatatggg 89580 atgcgtacgc cagtctgcat aatactttga gagaatgaca atccaatcta gatcaagcta 89640 tgaattacaa acggttatga agtaaaacgg gtgttacagc ttaaatttag ccagaataat 89700 ttaggagttt caacattgcc gtagaggtgt aacaactcac cagtggccat ccacatatta 89760 atatattcca taacttttaa ctaattttat ttgtaacgcg ggcaaaatat attttgttta 89820 agtctgcttg ctacgatctt agacataagc ttggttcaat actcgcgctt aaagaaaaat 89880 ttcaaaaaca gcaagttttt ggactctcaa attgttacaa aatgcaccct gtacgttagg 89940 ggaatggtta ctactttata tccattcttg tttagacaga agcacgattc ccatgttaat 90000 cctccaactt cattacattg aatatacgtt ccctttaatt ttctatttga tttaaggtca 90060 ttccatcatc taacactaaa tttctactga ttatttataa caatatgtag gaggattagg 90120 caagatgcga aatgctggtt gtttttggtt ctggacattt tatctcgaat taaatctgcg 90180 cattaatatg aattctatag attgacaagc agaaaacata ggtctcttta agacaatggg 90240 aataattaaa tacctagagc ataaattact gagtaaatat cttgactcat atcatagatt 90300 cctagccccc ggttgctagg aaggttagtc ttgtagtttc catagaccag actgctaaag 90360 aagagagtta ctgatatctt ttttttaggg cgacgtaatc ctagatgatg attcatatgg 90420 accaacactt agaatattac aaatcttcta gttacctaat atttttgatt tgcacctggg 90480 aaatcattgc attcgatttt agatcggtgt aaagattgat caaactccat ttaattttat 90540 ccaaatttac catatgcatg ggataacacg tattatgaaa ttcacttttc gttaggttac 90600 aactagttcg agacttattg tttgctaata ctacattatc ctcacattag cgaatgtaat 90660 tggctctcta aaaatcctca tagttgtaat gggaagccga gattattgag tgatcaccag 90720 tagctgtact attttataag ctattaaaaa ttgattaaaa taaacatacc gcctgtaata 90780 tataaatcat agtcagacga acattgctac aatctgtatt cgtaccaagt tatgagcgcg 90840 tatttcttat ttaagttttt gtcgctcaca aaacactggg tttaccaatc attcacatgg 90900 gacatgcgat cgtactgcct atgatgatat attggtaaga agttatctgt ctttcttcta 90960 agagagcaat taaaaggtta aagaaatgca atatatatgc aagggaaatt aatggataat 91020 cttaattcct gtaaggtagc acattgtgac ttaaaaatga ctaaataata ttgttgtaca 91080 tctagctaat ccgttctggg ctttacgacc aacagtaacg aagacctatt aaaaagacct 91140 tgatatggac gcgcaactat agttaagtta cctagatgtc cgtcttttct attcagccaa 91200 attctcttac ctttttcaat ctttggatct cataattaat agctcgttta tagaagagta 91260 tatactatct aagggtggag ggccaaggat ccttccaatc acaacatcaa agatatctgg 91320 actgacgttt tctcctctca aggactatag aaaaaaaatc ccattgcatt aggatctacc 91380 acaaagatta cctggttgta aattttgcaa tgttaagaac atcaatggat cataaaaata 91440 aaacgtgcat cctttagtaa caaaatctaa gatctagaca aatttccaac tagtctgaac 91500 ttagattaaa gaggtttaat aggtacacga actctgtcgt atatattact ttaagtgaaa 91560 tgttttccag tgttgatcaa tctctgaata acaaacgatt ataatgaaat tagagtgtaa 91620 acgttaacaa cgaccgagag atttttagca gcatcatagt tacctctcgg ctctagtatc 91680 tcacaaatgg tcatcgattt gataaattgc tcgataattt ctatgtactt tcatttgtat 91740 ggcggacttt ataaatttag tatcagtctg gtttaaacga tgttgagcat aagtgtgctt 91800 gaaaactgcc acataaaggg taaattcgaa aacaacgagt gttttgtgaa ccatatgcat 91860 aattagagta ccctgtacgc tagcatgaca catgcctcca caaagctatt cttcaataca 91920 gtataaatag attcattcgt ctatttttct atttatatgc gttatattta cgttgtcttt 91980 aattacctgc tagaattaaa gacattccat tgtgtaggac tgagttttca gtaattcatt 92040 atattaactt gaagaccaat taggcaagac ccgacaagtt ggttgtgaat gcctccgaat 92100 aattcttcta aaactatgcc tgggtgttga cagcaatcca ttgaaacttc aggcagaaaa 92160 gcttagccgg tttaagtgaa tggaaaaact tagaagcgtt ctgtattgat tatcgaggaa 92220 atattttctc atttgtgaca gatttccacc tcccggttct taggaaggtt aaaggtgtaa 92280 ttactattga cgtgactgca gaggagaaga gatcttgttt tcatttttta acggtaacgt 92340 gatcctgaat ggcgtttata atggagcaat atatttaacg tcggaattct tgtagttatc 92400 tagtattttt attttgtata taggagataa taattttaca ttcaagattt gtttcatgct 92460 taatcagtct tgaatgtaat ttctctaaat catccatgtg ccagagacag catatttaat 92520 gaaataaact ttacaaaagg tcataattag ttagggagtt atcgtatgtt aatattactc 92580 tgatctgata tttgcaatcg ctgctgtctc tccaatattc gccgtaatat caatagagag 92640 gcgagatcat actgtgtttt ccaacggcaa gtctatctaa ccagttatta gaggaacttg 92700 atagcagaca taccgcctga aatatttaca tcttagtcag attaaattta ctacgactcg 92760 taaacacacg attttttggc agtgtatttc gcatataagc ttttgttgct tacaggacac 92820 ttggtatacg taatgatctc atgagacatc tgttcatact gtgtaccgag gtgttccgat 92880 aaatagctat gttatattta tctatataag ccgataaaaa gacaaatata ggcattaaaa 92940 atgcaacaaa tattaatgaa cggtctcaat tagtgaaagg tatcgctcct tggcccatat 93000 ctcaaaaagc gatatattcg aactactgat gaagcccatc agggctgttc tatctagact 93060 tacgggagct tattttgagg tttttgttac ggaccgacaa ccgtcgtgac gtaactttga 93120 agtccgtctt tccggatcga ccgaattggt tcaccttttt gaatcttcgt aggattatac 93180 taatagttgc tttttgtaaa agtaaacact ccctataggt ggtggtctat gagtttttcc 93240 attatcctta ttattgataa ttctactcat gtcacctcta ctatagtaca aatttattat 93300 actaccattg cactagatct tacggcgaat actctctcat tacataaatt ggagcctcag 93360 ggacatcaat agatgataaa tataaagttt atgtcctcaa ttgtaaaatt gtaagttctt 93420 aacaaagtac gtattagtca gaacttacat taatgacatt aggtatttac acagtttctg 93480 tgatatagat tagttcattt caaatctttt tccgtattag ttaatgcctc aatagaatag 93540 aattataata acactatatt ataaacgtta gcgacgacag agaggttgaa agcggcattc 93600 tagtaatctg tccgttcgag aacgatataa aatgttcccg tacagattga taggttagta 93660 ttctccctga gctgtcgtct ccatgacgga ctttatagta gtacaatcgc tttaatttaa 93720 tacatggtca gcatttctgt gccaaaaaat cgtcaaataa agcgtatatc cgaatacaat 93780 gaatgtgcta tggaccataa gcattactag aatactccgc agacaactat gtcataaggc 93840 tccgcgacgc aatttaacga tactatatca ataaaaataa tcagctattt ttctgtttat 93900 atcgctaatt cttacgttga ttaatattgc ttgccggagt ttatcatctt ttatagggag 93960 gaggcccgta gaggatttta cggtatataa gcctaataac ttctaccaat ggtgccgtca 94020 aaatagatca gagtatccag aaataaaact ccaataagaa tggctgacac taggcagcac 94080 aggatcgaaa cttacgctac aaaggcctaa cagactgaac caagtggaaa aactaagaag 94140 tatcccaata tgattaactg tgaataagat attcattcgt gaggaatatc cgtcaccact 94200 tactcaaaac cgtaatagga ataataacta ttaaaatgag gacagcgtac ataatgtcat 94260 gctcgaataa tatggtcgta tggtgaacta gaatacctcc tatgagaaag ttaagaactt 94320 aacctgggtg tctctgttca tatcttctat ttgcatctgt aatacaggag tcaacatatt 94380 aacacacaag agttctttct tccatatgca gttatgtaag tcattgctgt attccataaa 94440 cctgtcaaag tcactatatc tggttatgta aagtctagag aaatgcatag tcatttatgg 94500 aaatatctca actaagtata attgtgataa aatgtatgta gtggctgctg tttttccaac 94560 tttcaccgtg agatgattaa acaagtacac tcttgttatt tattataagc tcatgtgtaa 94620 ctaccctatc acaagagcga tccgacaggt gaagcattaa caggaaactc atcacgtcag 94680 cgtaattaaa ttatacacca gtcgtaaaat cgcactttat tagcagttta acatgcgtat 94740 agtctaaccc tacatacacg atacctgaaa atcgtaatga acttagtaac ggatagttaa 94800 aacggtattc cgaagcggtg cattaattcg ctaaggtatg gttgttctta ttacacggct 94860 tagatacaat tatagtggca aggaatacaa ttagttatta ccaacggcga cgaatagtag 94920 tttatttcct tcctgcgggc tcctcctgaa atgccactta tacggcttat tgaagccgat 94980 tagtacaata gatttattca acctcaaagg tctatattcc cggcttattc ttagctgata 95040 tgtcgcgtcg agtcccgggt ttgagtgcgt taacaccgga ttgtctggct tagttcaact 95100 tttggatacc tcatcgggtt atactaactg acacttattc tttaaataag gttcccttat 95160 tgggactagt aactgagatt tgaaattatc cttatttcta atgatcttaa ctctttcgca 95220 tgaataacag tacgagctta ctttacgagg ttatcactta ttcaagtgca ggatactctt 95280 ccaattcttg aaaaggacgt agagagttga atgtattaga tgggcgtacg cattatgtgc 95340 tcacttgtat aattgtgtgt tttcaaggaa ggaagtatgc atcaattcgt tcagtatcga 95400 cataaggtat ttggaaagtt ttagtgacat agtccaatag atttttgatc tctttaccga 95460 tcagtaaata ccattataga gtcaattcag atttacgcta tttaacacac aacgccgagg 95520 ataaaagggc tgaaattgac agtctacaat attgttcatg tgtcaacaat taacaataaa 95580 cgaatacacg ttgatgcgat agtgctctgg ctaggccgtc cacttcgtat attttctttg 95640 agtagtgtag acgtattaat atagcatgta gccagattct tagcatgaaa cgatgattaa 95700 aatgtacgca taccacgttg agatgtatgt gctatagact tttagtgtta cctgaaacat 95760 agtccatcga ttttgccata agactacggc acgaaattaa gtgatttcac acctacaaga 95820 gaaatgtgcc gaatctatga taataacacc gttcgttgaa ttaatcaata atggtagcgg 95880 ttgcatatta tcaaatgaag gagccacacc cggggaggag tttacggtcg atatcccgta 95940 caacttcggc taatcatgtt atctaaataa gttggggttt gcagttgtca gggcgaaatc 96000 agattcgact atacagccca gttcagagcc ctaactcacg taatcgtgac cttacagagc 96060 gagtcaagtt aattacctat cgagtagctc aatatgagag agtgtgaata agaaatttat 96120 tgcaaggcaa taatcctaat tattgatagc aaactttgat aagaataaag aatagcacaa 96180 ttcagaaagc gtacttactg tcatactcga atgaaatgct ttattaaaga aaaagttgac 96240 gtcctatgag aagattaaga acttctcctg catctcacaa cctacttaat cttcggggat 96300 gggtaataga taggtgaaca tataaataga gataagtttc taccttcaaa cgtagttaaa 96360 caagtcatag ctatatccca caaacctttc ataatcactg tatcgggata tctaagaagt 96420 aatggtatgg ctagtgatca atcataattt gtcagttaag acaagatgcg gtaaattgtg 96480 tattgcgagt cctattgtct cgacttaaac tgtctgatgt tataacaaat acacagtagt 96540 taattgtcaa ttgcttatgt actgctacgc cttgtggaga ccggtccggc gcgtgaagga 96600 tataaatgaa actcgtcaca tccacattat tatatcgtac atcggagtac gaatcgaact 96660 tggcctctat ttttagatgc gtgcgctgca agtgtaaatg taccaaatct aaaaattacg 96720 atgggttttg tttcaggtat ccaaaaagta attctgatgc cgcgcttcaa ttcactaaag 96780 tgaggatgtt gtctttatac gggtttgata cttttattgt accaagcatc tttattagtt 96840 agcactatcc ccaacgtata acagtttact ttctcggtgt gagcccctcg tcaactgcct 96900 gcagtagtgc acgttgaagc cgattagaat attagatttt ttcaactcca aacgtcaaca 96960 gtcccgctct agtttaagtt catatatcag gacaggtctc ggaattaagt gtataagcgc 97020 tgggaacttt cgcgcagttc gaatagtgag taagtgatcg agttacacac cgttagattc 97080 atccttcaga taacgttccg tttttttgat ttataactat cgtttgaatc aattccatct 97140 tcttaacgtg ttaagtcatc cgcaaaaaag acgatatgag cttaccttac gaaataattt 97200 catttcgaac tacagggtgc tctacttgct cttgaagata tcgtagagtg ttggatgaat 97260 taaaaccccc tacccatttt caatcgactt gtatgtttat ccttaaacta acatggaaat 97320 ttgctttgat ttgtttagta tcatttaggc gtgtttcaaa tgtataatta agtatgccgc 97380 atagtttctc cattaccgaa ctgaacacca cttagtgtca aacactcatt gctgttctac 97440 gccatttatg acattacgct caaattaact gagctgaatt tcaaagacta taatattatt 97500 tttgtgttac caattaacag ttaacgaata cgtgacgatg tagaacacca ggagccaggt 97560 cggacgcatc ctatatttac ttcgtgcaga gtaggtctaa caagatagca tggaggttta 97620 tgcttaagtt gaaccgcaga taaaggtcta tactcgggat cttcactatc tgaaggttta 97680 gaattttaat ggtaccctat acaaagtgca taggtttttc attaatatta agacgcaaag 97740 gtaagtgaat tcactcctac aacggaaata tgaccaaagt atcaaaacaa tatggttcgt 97800 agaaaaaatc gatcgtgata aaggttggat tttgacaaaa caaagagcta cagtctggga 97860 acagtcgatg gacgccacca cgtgcttttt cggctaatct tataatttat aaatgttgag 97920 gtttagaatt ctcgggacga gatcaaattt aagtatatag tcatatccga agcgttaaat 97980 cacttattcg ccatgcttaa aagcgcctca tgcttttcac tgattcatta gctctatgta 98040 tggcaatcta tgtacgaagt ctgtcgtaag acttaaaaac taaatattgg tagcttcctt 98100 agttaaagta caaggattgc acaattcagt agacattttt tctggtattc ccgaacggaa 98160 agctttatta agacaaagtt tgatatccga cgggatgaac cacaagttct atgatatctc 98220 agaagctact aaatttcggg ggttgggtac aagttagttg aacatacaaa ttggaatttg 98280 attgtatcct tggtcgattc taaacaaatc gtagtaaacc cgtgcaaagt atatatatta 98340 tttcatacgg cgtttcaaag aggcaatggc ttgacatgag ctggatttca gattatgagt 98400 aaccacaaga tagtgtaaac actgtaatcc atatttaatt gagatctctt aaaaatgctg 98460 atattatttt ctaaactcga tggtttattg acaattattt gtacagtgat acatctagcg 98520 gttcttggtc gaccctactt acgtaataaa tgtagcacgt ttgatccaga ctcatccatg 98580 gtagcttcaa atacggattc aacttggcgt ttatatccag aaaacggccc tagaggtgat 98640 agacatttaa cactttggac aaccattgga tatgtttcac gtatcgatat agtaattatt 98700 attctgcatt tgctttctag taagtgagga agaagccttt ctattggttt cttaatcctc 98760 ttataccacg cgtgtatctt tcctagccct ttattgaaac tggaagtgtc ttgttattca 98820 atgttaaacc cttgtcagcc tattgcggtg atgcacgaaa aaaccaaatg gaatataaaa 98880 tattaactac tcctaatttt aagtgccctc ttctagattg tattcatata tcaatataga 98940 cttcgcaaac tagtgaatga gggaaatgaa tttatccgga ataacaaaag ggactgagta 99000 ttcaagatac aaaccgttag atagatgttc cagtctgcat agtaaatttt tgttttttaa 99060 ccattgaagg aatcaatttc acgatcctta cgagtttagt catcagaaaa agagaccttt 99120 agcgctcccc ccttgaaata gtacagcttc aaacaataac ctgagtaagt tggtatccga 99180 ggtactataa atttttcgat catttgatgc ccccatccta tgtttaaaga actattatct 99240 ttagccttaa gcttacacag gaaccagctg agtctaggtt aggttcactt gggttgctta 99300 catatataaa ataatgtata ccgcagaatt tgtcccttag ggaattgtag ttgaccaaaa 99360 gtctattact cattggcgtt gtatcacttt tgtgtcaata ggtatgatca atctcttgga 99420 aattattacg atgttggtaa aagattagaa ctactaaaca actgttaata atcaagtcgc 99480 tccgtagatt gccaagaatc agttgagatg aatacgttat ttagaacgta caaactacga 99540 ttgagttggt accatcgtag tttatgccta tgttaatccc caattatacg tcttttgccc 99600 gaatccccac tatctgtgaa atgtttaacg tgttcgttac ctttacaaaa tacatagcta 99660 tatcatacgt aaaaatatct taacggaaga tcgtacactc tttcttcgga tagataagca 99720 aagaaatagg aggatatggt gcacacatgg aaagggccgg taagtaattc tggccttcat 99780 agaacaatat gttataattt ggaaattgtc ggatagcgcg actatgtgct taattggttt 99840 actttaaatt ttataattga tgacaataaa aattcaccgg agaagttcta acgtaagtat 99900 atagttataa ctgaacgatt ccaagattta ctcccttaag ttaattagac ctatttgttt 99960 tccctgactc ctaagtttta tggttggcaa tttatctgca aggagacaca tatcatttag 100020 aaataaaaaa ttggaaactt ctttagctgt tgtgctggga acgctcatat caatactttt 100080 cttctctaga agttgaaagg ggggaatatt atcatgtcga agtttgttat aagagtcatt 100140 ctaccataca caccatgatt ctgaaagtgc tggtaaacta cgggaataag atacatattt 100200 ctcattatta gaattcagac ctcaaatgag tcgttggtca actcagatcc gaatcgaaag 100260 aacccaacga atatataact tttattacat gtgttgtctt agtcagagta ttccttaaca 100320 ttaactggtt ttcagacgat atgtaactgc agcagagtca aatcatggat attgatagta 100380 gttagagaac cattgataat gttacaacca ttttctaatt ttgatgttgt gtagacaatt 100440 attagttgag cgagctaact aacagttctt acaaatctct actcatacaa taaattccgt 100500 gagctcaaca ctaactcaac ctaggtaaca tcaaatgccg atatattcag gggttattat 100560 gcgagatatg cgcttaggga taatagacac tttacaaatt gcgcatgcga tcgagatatt 100620 ttatgaatcg atataatatg cattcttata gaattgcctg ttagcgtaac ataaataaac 100680 gtatctaatt acttttttat ccacgcttat tacatccgta ccatttccgg ccattcatta 100740 agatcggaag taccttgcaa tacaaaatta aactttaaat accctatcac gcagatttag 100800 caattatttt aatcaacttt agtggatcaa tcatagacat ttttaagtgg tattttcaag 100860 gtggcattta aatattaatg atgacatatt aaggttttaa atgagagaat tcaattaatt 100920 tggttagaca aagggatccg aagatatttt atcgcaacca ttaaaagggc gtacctctgt 100980 ctacaataag cctttatttt taaatgtttg aagaaatcgt gaagacgact gttgtcaata 101040 caaatatgat aagaacagta ctacatcttt gctccgcaat aatagtacag cttcaagtaa 101100 tattcacagt aaaatggtat gtctggtact aagactttca tgactgttcg acgtttatac 101160 tttaagtata aagagtagtg atccgatgtc tggagattat tctacatcca attgactcta 101220 agcacagcct tcttggcttg ctagtacatt gaaagtgata tactcaactg gacgagactc 101280 gaaaggaaat atgattgacc ataagaccgc tttgtttcga catcgtctta gtacagtact 101340 cataattatc atcattcttt tagtgcctat tacaatgttg gcaaaagaat aaatctccat 101400 cacacttctt gaaaatgtac ttggtcgttt gattgttgag aaagtttaat gatgagtatg 101460 ttatttaagg cactggtgta ctgatagagt tggactaaat gttaattacg agtatataac 101520 tcttcaataa ttcgctctat acgcaaattc ctattatctg tgaaatgttt aacgcctatg 101580 gtaactctgt aaaatattta tctacgttat accttagaat atcttaacgg actatcgcaa 101640 tatatctact tgtactgatt aatgggaaag taggtgcgat aatcgaaggt atggtaaacg 101700 ccggtaagta attctaggca acaaggaatg ttattttttt aattattttt taaggcattg 101760 tacgtctaaa tcatcaataa aatttgttga aatcaccttg tttgattttg taaaaatttg 101820 tcttaaaact atcaccgtta attaaagatt actacactat aattccagaa tttacattct 101880 taagctattt aaactaatct gtttccctag gcttctataa aatcgcgtca gtgattatcc 101940 cccttggaga cggatgtttt tcgcaaataa caattaacaa atttctatgt cacttctgct 102000 gtcagcaatt ttgtttttac tatacttgtt atgatgtaga ttcgtcgcgt tattgcgttg 102060 tcgaacttca taaaaagtgt ctttttacca tgcagatcaa gattgtgaaa gtatagacaa 102120 gcagcaaata cgaaaatcat atttttgatc acatggccac agacctctag taggaagaac 102180 gtttactgag gttcgtgtcg gtagtaccaa acattcatgt aactctgaca atgcgaattg 102240 acctgctttg agctcttcta tatactaact agcattctgg cgaaacaaag ctgtggtagt 102300 attgtgtcat gagtactaat agtagtaaaa aaatcaggga taatgtttcg acggttcact 102360 tatttagagg tagtgtgaga tacttttacg tgaactagca aaataacatc tcttttaaat 102420 tatttgtctt acaattactt ccgtgatgac atgattatct caacccgata tacaattgat 102480 gttctcatat tgaaaaatta ttaagccaga tatgtattaa agcatagtac atacatcaca 102540 agttccggat atcgttaaga ctatttataa ataattccat tgtggaatct tataatataa 102600 cctgatagcg ttatgttggt gaagatggca aattacgctc tcaccggcgc tattagcttc 102660 cataccagtt ggcaacattc attaagatcc gttattcctt aggaaaaaaa tatttataag 102720 aaatcctgta acatgtgcat ttaatagttc taattaacaa cattactcga gcaaactaaa 102780 acttttaaac acagaatttt agcagaggct aataatatga tatgatatga aattagggtc 102840 tatatagtta taatttgata aattaaatta gattaggagt ttggaagata atttagcaca 102900 gttagttata gacgaaggat ttgccttcaa aaagcggtta ttgttatttg tcttaagttt 102960 cagcgaaaac aggagtcgtt aaaacaaaaa taatatgtcc aatattacat ctaagcaagg 103020 caatttcgca acaggttaac ctattttttg tagaaaaatt agaggtccag ttcaaacact 103080 ttcatttgtg cagctcgttt atgcatatgg tgtgtaaact ggtgcgtcgg tgtatggaga 103140 ccttccttct tgcgtatgat tgcatggact gcgatcacgt tttgtaagtt cattgagact 103200 gttacgctta attgctcgga attcgagaaa ataaacgact attcgtaata acgctttgat 103260 cgaactcgat cataacagag cacacatgat aatcttcact tttttagtcc ctattacaaa 103320 gcagcggagt gaatatatgt ctatcagtca ctatgaattt gcatatgatc gttttactgt 103380 agaattaatt taataaatag aatgtataag aaggatctac tgtacttata caaccaggct 103440 acatattaac tacaagacta tatcctatta ttgacttagt gtatacataa tatggtttca 103500 tacatgtagt gttcaagggc tatagcaaat ctgatgataa atgattaggg taacacctta 103560 gaattatata ctagcctatc ggaacacaag cacttttgcc gtataatccg agtgtcgcca 103620 cgataatgga aggtatggtc gatcgttgtt gctattagta ggcgataacc tatccttttt 103680 tcataaattt tctttagaac attgtttaca aaatatatca ggattaattg atgtaatgag 103740 ctcgcttgat tttgaatact agtgttttta attcgactcg gattaataca ctatactaaa 103800 ttttaaagcc agatatatca atattaaact acttaattta acctaatcgt caaacgatct 103860 attaaaccgt gtctatcagt atctgcttcc tgaatgatac tttatcgcca aaatcaatag 103920 ttagaaccta aagtcgcttt cgtccttagc tacatcgatt tctttataca ggtagtaacg 103980 taaattcatt ccgtaaaacg gtttttcact tggttataaa acatttcttt gatctccagg 104040 agaaatatga gatactaaac acgtcgagaa tttacgtttg ctgctcattt gtccacgcac 104100 ccacatacct ctggaaggaa aaatacatac taacctacct gatgctagtg taaaataccc 104160 aagagacgct gacagtgcga tttaacgagc ctttaactgt tacattagct gataaacatt 104220 attgcgtgac tagcttgacc tcgtattgcc cagtgtgtag tattagaagt gataaaatca 104280 ggcttaataa gtcgtggcgt catttacata ttgttagccg gtgacaggta aatgtatagt 104340 agcataacgt aatcttaatt aaattattta tcttacacat tcttcctaga tgacatgaat 104400 atactggtac gatgtataat tgaacttttg atataggata agaactggaa cacatatata 104460 ttatacttat gcttgtacat cctaagtttt caatatcgtt tagggtacaa tttacaaatc 104520 cctttccgga atcataatat aagatcggat aaccttgtgt tcgtggaaag ggtacatttg 104580 ggtcacagta gtggtattac ttatgaaact agctagcaat aacgaaaatc atgtactact 104640 gagcaggaaa attgtattta aaagatgact tataagtaat gttttacatg gtcctaattg 104700 attacattac tcgggggaac taaaacccat gaccactaaa ataaagctga gtctaaatgt 104760 gtgatttgat ttaaaatttc gatctatata tttataattt cgagagataa attggatttg 104820 ctatttgtta gtcgatttgg cacagaatga ctcagacgaa gaacttacta tgaaatggcg 104880 gtttcagttg tcaatgatgg atttcagcga aactaggaaa cgatgtggct taagaaatac 104940 ctctctcatt gcatttagat aaaacatctt gctaaaaaaa gaaccagtac tttgtaaagt 105000 aactagagat tcttttaata ctctgtgatt tgtccagttc ttaaaagcaa acgaggagta 105060 atcaggtgcg tgggtaaaag caaagctaca tttttaagta taatcagata gactacgatt 105120 acattatatg ggatctgtgc gactgtcacg gtaatacact cggaaaatga caatccaatc 105180 cacaactagt tataacccac tgatcgtaat ggagcataac gggtctcaca tcataaattt 105240 tactaattta atccgaattt ttcaacattg cggtagacgt gtaacaaccg accactggcc 105300 atctacatat tattatattc cataagtttt aacttaatat atagaaaacg cgagtaggat 105360 ctactgtact tatatcacct tgctacatat taagatgtaa gctaagtcca atacttgtcc 105420 ttgagcataa atttcaaaaa taggaagttt ttggattcag aagttatagc aaaacccacc 105480 ctaaacgttt ggggaatggt tttagcatta tatcctaacc tattagaaca gaagcaccat 105540 tcccatgtta accctgcgac ttcatcacat tgaatacttt tttcgattaa tattgttttt 105600 aatttatgat cactccacct tctaacataa attttctact gattatttat aacaatatgt 105660 acgaggatta gccaaggtgc cgagcgcccg tggttttggg tactggagat tttatttcga 105720 ctcaaatctg cgcactaaag taaattttaa agttagacat acagataata taggtctcta 105780 tatgacgtaa aggataatca aatagctaaa gcgtatgtta ctgagtctgt ttcttgagtg 105840 ataccttagg ctcttagtcc acggttacta gcaagactag ttttgtactt tcgatacacc 105900 gaactgctta tgaagagagt aacataaatc tattttctag aacgacttta tcctaggtca 105960 tgaaacattt cgttcaactc ttagaatatt acaaaacatc aagtagccca agatttttgg 106020 tttgcacctg attaatccac gcacccattt ttagatcgat gtaaagattc attaaagtct 106080 atttgatact attcaaatat accctacgca tgcggtcaga cgtattatga gactctctta 106140 ctgttaggtt aggtcttgtt cgatactcaa tgtctgccaa tacttcattt tgcccacaat 106200 gtcgaattta aatcggtcat attaggtcct caaagttgta acggcatctc cacattgttg 106260 agtggtcacc ggtaggtgta taataatata aggtattgaa aattgattaa aataaacatt 106320 gcgcccgttt tatataaaac aaattcagtc gaacgatgca agaatctgta ttcgaaccaa 106380 gttatgagcg cgaatttctt tttaaagttt ttgtcgttca aaaacctgag agtttaacaa 106440 tgttttacgt gggacatgca atccccttac caatgatgaa atataggtaa gaacaaatct 106500 gtcttcgtgc taagggtaca attaggaggc tgaagtaatg taacttatat gcaagggaaa 106560 ttataagata aattaaattc cagtaaggta gtagattgtg atttaaagat gactaataaa 106620 tattgttata catcctccta atcggttcta cggtttacga ccatcaaaat ccaagacctc 106680 taaaatagag ctaaatttag acgcgtgatt atacttaaga tatcaaactg ttcgtctatt 106740 gtatccagag atattctgta tcccttatta atttatggat ctcgtattca atgactcatt 106800 tatagaactg agtatagaat ctaaggatcg ggggccaatg atccttccga tcagaacatc 106860 aaagctatct ggtctgacga tttcttctct caatgacttt agaaaaaaaa tcccgctgca 106920 ttaggatcta gtactaagta taccaggttg tgaatcttat aatgtttaga agatcaatgg 106980 attttaaaaa ctaaacgtgg accctttagt aacgtaagct aaaatctagc cacatttcta 107040 actagtttga ggtaaattat aataggttta tatggtatac gtaccctatt gtgcataata 107100 ctttaagtga aaagcaatcc aatgttgatc aagctttgaa taacaaacga ttatgatgta 107160 ataggagtgt aatcgcttat attaaccgag agatttttag gagtatcaac attacccttc 107220 ggctctaata actcactagt ggtcatcgac atgataaaat attcgataat ttttaactaa 107280 ttttatttgt atggcgggca taatatattt agtatgagtc tgcttgtaac gatgttagac 107340 ataagcatgg ttcaatactc gcgcataaag aataatttca aaaacagcga gtgtttcgtg 107400 tcccaaatag ttagtaagtg taccctgtac gctagcatga cggttactac tatataacca 107460 ttcttcaata gacagaaaga agattctctc gttaattttc caacttcttt acgttatata 107520 tacgttccct ttaattacct attagaatta aggacattcc atcgtgtaac actgaatttc 107580 tactgattta ttataacaac atgtagatgg attaggcaag acccgaaatg ctggttgtca 107640 ttgcttctgg ataatttttc tcgaactata cctgcgcgtt gatatcaatt caatggatct 107700 acaagcagaa aagataagtc ggtttaagac aatgggaaaa gttagatacc tagagtatta 107760 attatcgagc aaatatcttc tcatatatga tagattccta cctcccggtt gctaggaagg 107820 ttagtcttgt agtttctata gacctgactg caaaagagga gagttcctga tatctttttt 107880 ttagggtaac gtaatcctag atggtgtttc taatggacca acatttagaa cgttacaatt 107940 cttctagtta cctagtattt ttattttgca cgtgggaaat cattgcttta gattctagat 108000 ctgtataaag attgatcaga cttgaatcta atttgtccaa attatccatg tgcttgagac 108060 agcatatatt atgaaattca ctttacaaaa ggtcacaact agttagagac ttattgtttg 108120 ctaatattac tttaatctca catttgcaat tgttgctggc tctctaaaaa tcgtcgtagt 108180 atcaatggag agccgagatt atagagtgtt taccagtagc taaactattt aacaagctat 108240 taaagataga tcaaagtaaa cataccgcct gaaatattta aatcatagtc agaccaaatt 108300 tgctacaact cgtattcaca ccaacttttg acggcgtatt tcccatttaa gcttttgttg 108360 ctcacaaaac acttggtata cgtattaatc tcatgggaca tgcgatcgta ctgtgtacgg 108420 aggtgtttcg gtaagaagtt atgtcatttt tatctaagta agcagataaa aagataaaaa 108480 tatgcaatat aaatgcaaga gaaattaatg gacgatctta attcctgtaa ggtaacacat 108540 cctgactcaa aagtcattaa ataatataat tgaacttctg gttaatccgt tctgggctgt 108600 tcaaccaaca gttacggagg cttattaaga agattttgat acggacccgc aactatcgtt 108660 aggtaacttt gaagtccgtc ttttcgaatc agccaaattc acttaccttt ttgaatcttc 108720 gcaagtcata actaatagct cctttataaa agagtaaaca ctgtctaaag gtggagggcc 108780 aagaatcctt ccaatttcca cattaatgat aactgcactg acgttttctc ttctctagga 108840 caaaagtaaa aaattgccat tgcactagga cttaccgcaa atattacctc gttatatata 108900 ttgcagcctt aagaacatca atagatcata aaaataaaac atgtatcctc tattattaaa 108960 atgtaagttc taaacaaagt acgaattagt ctgaacttac attaaagaga tttagtaggt 109020 acacggtctc tgtcgtataa attactttat ttgaaatgtt ttccagtatt aatcaatccc 109080 tgaatagcat acaattataa tgagactaga ctataaacgt tagcgacgac agagagatta 109140 taagcagcat catagttatc tctccgctct agtatcacac aaatggtcgc cgttcagata 109200 gattggtcaa taatctccat gaactatcgt ctgtatggcg gactttataa atctagaatc 109260 agtctaattt aaatgatgtt gagcatttgt gtgctaaaaa accgtcacat aaagcgtata 109320 ttcgaaaaca acgaatgtcc tgtgaaccat atgcattatt agagtactct gtagacaagt 109380 atgacacatg gctccacaag gctatttatc gatacaatat aaatagatat attcggctat 109440 ttttctgttt atatccgtaa tttttacgtt gtttttaatt acttgccaga gttaatgact 109500 ttccatagcg aggaaccggg tattgagttt ttcactatat aagcttgatg actactttgg 109560 gtagtcccga caagttagat ctgaatgccc tcgaataata ctccaaaaac aatgcctggc 109620 tgttggcagc actgcattga aacttcaggc agaaaggcct agctggctta accaagtgga 109680 aaaacttaga agcgtcctat tatgattatc aacgaaaaac attttcattt gtgagagata 109740 tccaccaccg gatactcaaa aaggttatag gaataataac tattaagatg agtacagtgg 109800 agatgatatc atgtttagat aatatgacgg taacgtgatc tagaatgccg cttatgagag 109860 agtaatgtat ttaacgtcgg agtccctgta gttatctact atttatattt caaatacagg 109920 agttaacatt ttaacattca agaattgttt catgcataat cagtcttgaa tgtaattact 109980 gtaattcata aatgtgtcaa agacactata tctaatcaag taaagtttag aaaaaggcat 110040 aatcagttac ggagttatct tatcttaata ttattctgat ataatatttg caatcgctgc 110100 tgtctctcca actttcgccg taatatcaat agacaggcaa gctcttgcta tatttttcaa 110160 gggcatgtct aactatccaa tcataagagg gactcgacag cagaggtact gcctgaaata 110220 tcatcatgtt agtgaaatta aattaagtac cagtcgtaaa gacacggttt tttagcagtt 110280 tatttcgcat ataagcttat gttacttaca cgatacctgg tattcgtaat gatcttatga 110340 ggcgtctgtt gatacagtat tccgaggcgc tgcgttaaat tgctatgata tagttatttt 110400 tattagtcga taaaaagaca aatatagcga ttaagaatgc aacaaattat aacgaacggc 110460 ctcaaatagt agaaaatatc cctcctccgg ccatctccta aaatgccata tattcggatt 110520 attgatgatg gtcaccaggg cagttttatc tagtctcata ggtgtttatt ttgaggttat 110580 tcttaccgac tgtgaaccgt cgtgtcgtag ctttgaaccc ggtgtttccg gattgtctga 110640 cttggttcac ctttttgatt cttcataggg ttatactaat tgacacttat tctaaaagta 110700 aacactcctt ataggtagtg gtgaatgagt tttagcatta tccttattat tgataatttt 110760 actcttgtca catgtattac agtacgaact tattatacca ccataccact agatcttatg 110820 gagaatactc tctcaatact tgaattggac ccacagagac aagtatagaa gataaacgta 110880 gagattatgt cctcagttgt ataattgtga gttctcaaca aagaaggtat acgtcaatac 110940 attcagtaac gacataaggt atttggacag tttcagtgat atagaccaat acatttcaga 111000 tctctttacg tatcagtaaa tgcctctata gagttgattc atattaacac tattttacaa 111060 acgtcaccga cgacagagag gttgaaagcg gcactctact aatttgttca tgtgagaaca 111120 atatataata ttcgagtaca cattgatggg atagtgttct cgctaggctg tccactccgt 111180 aattgtcctt aaagtagtac agtcgcatta atttaatacg tggtcagcat tttagcgtga 111240 aataatcgtc aaattgtacg catatcagat tgggatgtat gtgctatgga cttttagcat 111300 tacttgaatc attccctatc aattttgcca taaggcttcg ccacgtaatt aagcgattcc 111360 ataccaataa gaataatgtg ccgaatcttt gttaatatca ccgattctta cgttgatcaa 111420 taatgcttgc cggtgcttat catcatttaa aggaaggacg cccgaggagg actttacggt 111480 aaatatgccc aataacttcg gctaatgatg ttatctaaat aagttggagt ttccagatat 111540 aagggccgaa taagaatcga ctatacagcg cagcacaggg tcgaaactca cgcaattgtg 111600 gcctaacaga ctgaatcaag tcgaaaacct atggagtagc ccaatatgat tgactgtgaa 111660 taagaaattt attccaaggg aataaccctg atcattgact ctaaactcta ataggaataa 111720 agattactag aatggagaaa gcgtacttat tgtcatgctc gaatgaaatg ctccaatagt 111780 gaataagttc acctcctatg agaaggttaa gaacttttcc tgcatctctc aacttacata 111840 atctacccgc atgcgtaata cacgagtcaa catattaaca cacaaaagtt ccttccttca 111900 tacgcagtta agcaagtcat agctgtattc cataaacctt tcaaaatcac tgtatctggt 111960 tatctaaaaa ctagagaaat ggctagtcat ttatggtaat atctcagtta agtttaaatg 112020 cgataaattg tgtgttgcgg ctcctatttt cccgacttta actgtcagat gataaaacaa 112080 gtacacactt gttaattgtt atttgcttat gtgcaactac gctatcacga gaccgatccg 112140 acaggtgaag catataaaag aaactcatca catctgcata attatattgt acatcggtct 112200 tagaatcgta ctttgctact aattttacat gcgtatggtg caactctaca tacacgatat 112260 ctgaaaatca caatggactt tgtatcaggt agctaaaacg gtattctgaa gccgtgcttt 112320 aattcactaa agtgtggatg ttctctttac acggcttaga tactattata gtggcaagca 112380 acataattag ttattaccat cgccgacgta taatagttta cttcctcggt gtgggcccct 112440 cctcaaatgc cacctatagg gcatgttgaa gccgattagt acaatagatt tattcaaccc 112500 caaacgtcaa cagtcccgct ttagtctaag ctgatatgtc gggtcaagtc tcgggattga 112560 gtgcattagc actggaatgt ctcgctcagt tcaattattg gatagctcat cgagttatac 112620 tctctcacac ttattcttta aataacgttc cgttattagg attaataact gtcgtttgaa 112680 actattctta tttcttatcg tgttaagtct ttcgcatgaa tgacagtatg agcttacttt 112740 acgaaataat ttctttttca actgcaggat actcttctaa ttcttgaaga ggacgtagag 112800 tgttggatgt attagaagcc cctacccatt atctatccac ttgtatattt atctcttttc 112860 aaagatggaa gtttgcatca atttgttcag tatcgatata gggtgtttgg aaagtattag 112920 tgacatagcc caatagattc ttcattacca taccgatcag taattaccat taaacagtca 112980 attctgttct acgccattta acacataacg cccaggataa cagagctgaa tttgacagac 113040 tacaatattg tttatgtgtc atcaattaac agttaacgaa tacatgacga tgcggaacac 113100 ctctggccag gccgcgcact tcgtatattt actttgagca gtgtaggtgt aataatatag 113160 catgtagcca catccttagc ttgaaccgga gataaaaatc tacgcacgcg acgttcacat 113220 ttatatggtt tagattttta gtgctaccca aaacaaagtc catagatttt tcattaagac 113280 tacggcgcga agttaagtga tttcacacct acaagagaaa tatgcccaaa ctatgaaaat 113340 aacatcgttc gtagaaataa tcaatcgtga taggggttgc atattgtcaa atgaaagagc 113400 cacactcggg gagcagttca cggacgtcat ctcgtgcaac ttcggctaat catataatct 113460 aaaaaagttg aggtttgcag ttgtcagggc gaaatcaaat tcaagtatat agtcctgttc 113520 agagccttaa ttcacgtatt cgcgaccctt gaaagcgcgt caagcttatc actcattgac 113580 tagctcaatg tgtggcaatc taaataagaa gtctattgca aggcaaaaat gctaattatt 113640 gatagcaaac ttagataagg tagaagaatt gcacaattca gaaggcgttt tttctgctat 113700 actcgaatgg aatgctttat taaagaaaag cttgatgtcc cacgagatga acgagaactt 113760 ctatagcatc tcacaaccta cttaattttg ggggatgggt aaaagttagc tgaacataca 113820 aataggaata agattgtacc ttcaaacgaa actaaacaaa tcatagttaa tccgcacaaa 113880 gtttacatat taattcttac ggcatatcaa agaggtaatg gcttgacttg tgatgaatca 113940 tagtttgtga gtaacgacaa gatgcggtaa atactgtaat gcgagtttta ttgactcgac 114000 ttaaagtttc tgatattata ataaatacac agtggttaat tgtcaattgc ttatgcactg 114060 ctacaccttg tggtcctcgg tccagcctgc gtaggatata aatgaagcac gtctcatcca 114120 gattgttcta tcgtacttcc aaatacgaat tcaacttggc gtctatttcc agatatgagc 114180 cctagaagtg atagacttcc aaatctaaaa attaccatgg gatatgtttc acgtatccaa 114240 aaagtaatta taatactgcg tttccattca cttaagtgag gatgttgtct ttatactggt 114300 ttcatagttt tgttgtacca agcatctttt ttagttagca ctatttccaa cctaaaactg 114360 ttttgtttct cgatgtgaga cccttgtcag ctgcctgcgg tggtgcacgt aaaagccgat 114420 tagaatatta aatatttaca actccaaatc ttaagagccc tgctctagtt taaattcata 114480 tatcagcata ggcttcgcaa tttagtgaat aagcggtacg aactttcgcg gagtacgaaa 114540 agtgactaag taatcgagat acataccgtt agattcatgc ttcagacagc attctgaatt 114600 tttgatttat aaccatcgaa ggaatcaatt tcatgttcct aacgtgttaa gtcatccgca 114660 aaaaagacga taagagctac cagtgcattt taatttgctg ttatattcgt tatagttatg 114720 atatcatttt ctatagcctc taccacttaa tcctatgaaa cttgcgtttt aagtattttc 114780 actatgtggc ggtcttttag gtcagttact atgcgaaacc atactatgtt cattgggact 114840 acaacgacag aacgcatcta taaccttacc agctacctaa cttcgttgtg gtttactaca 114900 actttttaat ccaatctatt gttctctctt ccgcgataag tcacttaatt tgttattaaa 114960 ctaattaaat agatatgttg ttcgattcct aagtacatac atcacaagtt cccgatatcg 115020 ttaagactac tttttaataa ttccattgtg gaatcttatt atatgaccgg atagcgttat 115080 gttggtgaag atggcatatt aggctctcac cggtgctatt agcttccata ccagttggca 115140 acattcatta acatccgcta ttccttagga aaaaaatatt taaaagaaat cctgtaacaa 115200 gtgcatttaa tagttctaat taacaacatt actcgagcaa actaaaactt ttaaacacag 115260 aaattaagca gaggctaatt atgtgatatg atatgaaatt agggtctata tagttataat 115320 ttgataaatt aaattagatt aggagtttgc aagataattt agcacagtta gttatagacg 115380 aaggatttac tttcaaaaag cggtttttgt tatttgtctt agatttcagc gaaaacagga 115440 gtcgttaaaa caaaaaaaat atgtccatca ttgcatctaa gtaaggcatt ttcgcaacag 115500 gttaacctat tttttgtaga aaaactagag gtccacttca aactctttga tttgtgcagc 115560 tcgtttatgc atatggcgtg taaactggtg cgtcggtgta tggagacctt cctttttgtg 115620 tatgattgga tggactgcga tcacgttttg taagttcttt gggactgtta cgcttaattg 115680 ctcggaattt gagaaaataa acgactattc gtaataacgc tttgatcgaa ctcgaacata 115740 acagatcaca catcataatc ttcacttttt tagtcccaat tattgagcag cggagtaaat 115800 atatgtctat cggtcactat ggatttgcat atgatcgtat tactgtagaa ttaatttaat 115860 aaatagaatg tataagaagg atctactgta cttatacaac cacgctacat attaacttca 115920 agactatatc ctattattga ccttgtgtat acataatatg gtttcataca tgtagtgttc 115980 aagagttata gcaaatctca tgttaaatga ttagggtaac gccttagaat tatattctag 116040 cctatcggaa cacaagcact tttcccgtgt aatccgagtg tcaccacgat aatggatggt 116100 atggtcgatc gttgttgcta ttagtacgcg ataacttatc cttttatcat aaattttctt 116160 ttgaacattg tttacaaaat gtatcaggat taattgatgt aatgagctcg cttgattttg 116220 gatactagtg tttttatttc gactcggatt aatacactat actaaatttt aaagccagat 116280 atatcaatat taaagtactc tatttaacct aatcgttaaa cgatctacta aaccgtgtct 116340 atctgtatct gcttcctgaa tgatacttta tcgccaaaat caacagttac tacctaaagt 116400 cgctttcgtc cttagctaca tcgaattctt tatacaggta gtaacgtaaa tctattccgt 116460 agaacgattt ttctcttggt tataaaacat ttctttgatc tccaagagaa atatgagata 116520 ctaaacacgt cgagaattta cgtttgctgc tcatttgtcc acgcacccat attcctctgg 116580 aagtaaaaat acatactaat ctacctgatg ctaatgtaaa atacccaaga gacgctgaca 116640 gtgcgattta atgagccttt aactgttaca ttagctgatg aacattattg ggtgactagc 116700 ttgacctcgt attgcccagt gtgtagtatt aaaagtgata aaatcaggct taataagtcg 116760 tagcgtcatt tacatattgt tggccggtga caggtaaatg tatagtagta taaggtattc 116820 ttaattgaat tatatatctt atacattcat cctagatgac atgaatatac tggtacgatg 116880 tataattgta ctttcgattt aggttaagaa ctggaacacg tatatataat acttatgctt 116940 gtataaccta agttttcaat atcgtttagg gtaggattta caaatccctt tccgaaatca 117000 taatataaga tcggataacc ttgtgttcgt ggaaagggta catttgggtc acagaagtgg 117060 tattacttat gaaagtagct agctataacg aaaatcatgt actactgagc aggaaaattg 117120 tatttaaaag atgacttata agtaatgttt tacatggtcc taattggtta cattactcgg 117180 gggaactaaa acccatgacc actaaaataa agctgagttt aaatgtgtga tttcatttaa 117240 aatttcgatc tgtatgtcta taatttcgag agatatattg gatttgctat ttgttagtcg 117300 atttggcaca caatgactca gacgaagaac ttactatgaa atggcggtat cagttgtcaa 117360 tgatggattt cagcgaaaca agaaaaccat gtggcttaag aaatacttct ctcattgtat 117420 ttagataaaa catcttgcta aaaaaagatc cagtactttg taaagcaagt agagattctt 117480 ttaatactct gtagtttgtc cagttcttaa aagcaaacga ggagtaatca ggtgcgtggg 117540 taaaagcgaa gctacatttt taagtataat cagataaact atgattagtt tatatgggat 117600 ccgtgcgact gtctccgtaa tacactcaga aaatgacaat ccaatccaca acaagctatg 117660 acccactgat ggtaatggag cataacgggt ctcacagcat aaattttacc agtttaatcc 117720 gagattttca acattgcggt agacgtgtaa caaccgacca ctggccatct acatattatt 117780 atattccata acttttaact taatttatac gaaacgcggg taggatgtac tctatttaaa 117840 tcaccttgct acgtatttag atgtaagctt agttcaatac ttgtccttga gcataaattt 117900 caaaaatagc aagtttttgg attcagaagt tattgcaaaa cccaccctat acgtttgggg 117960 aatggtttta gcattatatc ctaacctgtt tgaacagaag caccattccc atgttaaccc 118020 tgcgacttca tcacattgaa tactctttcc gattaatatt gtttttaatt taagatcact 118080 ccaccttcta acacaaaatt tctactgatt atttataaca atatgtacga ggattagcca 118140 agatgccgag cgcccgtggt tttgggtact ggagatttta tttcgactca aatctgcgca 118200 ctaatgtgaa ttttatagtt tgacaagcag ataatatagg tctctataag acatagagga 118260 taattaaata gctaaagcgt atgttactga gtctgtatct tgagtgatac cttaggctct 118320 tagtccacgg ttactagcaa ggctagtttt gtactttcga tacaccggac tgcttatgaa 118380 gagagtaaca aaaatctatt ttctagagcg actttatcct aggtcatgaa acatttcgtt 118440 caactcttag aatattacaa aacttctagt aacctaaaat ttttggtttg cacctgatta 118500 atctacgcac tcgtttttag atcgatgtaa agattgatca aagtctattt aatactattc 118560 aaatataccc tacgcatgcg gttagacgta ttatgaaact ctcttactgt taggttagat 118620 ctagttcgat acttaatgtt tgccaatact tcattttgcc cacaatgccg aatataaatc 118680 ggtcttaata aatcctcata gttgtaacgg gaactccaca ttattgagtg gtcaccggta 118740 ggtgtataat tatataaggt attgaaaatt gattaaaata aacattgcgc ccgttttata 118800 taaaacaaac tcagacgaac gatgctagaa tctgtattcg aaccaagtta tgagcgcgaa 118860 tttcttttta aagtttttgt cgctcaaaaa cctgagggtt taacaatgtt ttacgtggga 118920 catgcgatcc cgttgccaat gatgaaatat aggtaagaac atatctgtct tcgtcctaag 118980 ggtgcaatta gaaggttgaa gaaatgtaac atatatgcaa gggaaattaa aagataatct 119040 aaattcctgt aaggtagtag attgtgattt aaagatgact aaaaaatatt gttgtacatc 119100 ctcctaatcc gttctacgct ttacgaccaa caaaaaccaa gacctgtaaa aaagagctta 119160 atttagacgc gtaattatac ttaagatatc taactgttcg tcttttgtat ccagacaaat 119220 tctgttaccc tttttaatct atggatctca tatttaataa ctcatttata gaactgagta 119280 tactatctaa ggatcggggg ccaacgatcc ttccaatcag aacatcaaag gtatctggtc 119340 tgacgatttc ttctctcaat gactatagaa aaaaaatccc gctgcattag gatctactac 119400 taagtttacc tggttgtaaa tcttgtaatg tttagaagat caatggatta taaaaactaa 119460 acgtggaccc tttagtaacg aaagctaaaa tctagccaca tttctaacta gtttgaggta 119520 aattaaaata ggtttaattg gtatacgtac cctattgtgc ataatacttt aagtgaaaag 119580 ctatccaatg ttgatcaacc tctgaataac aaacgattat gatgtaatag gagtgtaatc 119640 gcttacatca accgagagat ttttaggagt atcatcatta cccttcggct ctaataactc 119700 actagtggtc atcgacatga taaaatattc gataattttt aactaatttt atttgtatgg 119760 cggacattat atatttagta tcagtctggt tgtaacgatg ttagacataa gcgtggttca 119820 atactggcgc ataaaggata aattcaaaaa cagcgagtgt tttgtgaccc aaatggatag 119880 taagagtacc ctgtacgcta gcatgacgca tacttctata tagccattct tcaatagaca 119940 gaaagaagat tctctcgtta attttccaat ttttttacgt tatatatacg ttccctttaa 120000 ttacctacta gaattaagga cattccattg tgtaacactg aatttttagt gatttattat 120060 atcaacatgt agaccgatta ggcaagaccc gaaatgttgg ttgtcattgc ttctggataa 120120 tttttctgga actatacctg cgcgttgata tcaattcaat gaatcttcag gcagaaaagt 120180 taagtcggtt taagtgaatg gaaaaagtta gaaacctaga gtattaatta tcgagcaaat 120240 atcttctcat atatgataga ttcccacctc ccggttctta ggaaggttaa tgatgtagtt 120300 tctatagacc tgactgcaaa agagaagagt tcctgatatc ttttttttag ggtaacgtaa 120360 tcctaaatgg tgtttctaat ggaccaacat ttaaaacgtc acaattcttg tagttaccta 120420 gtatttttat tttgcacgta ggaaatcatt gttttagatt ctagatctgt ttaaaggttg 120480 atcagacttg aatctaattt ctccaaatta tccatgtgct agagacagca tatataatga 120540 aattcacttt acaaaaggtc acaactagtt agggacttat tgtttgctaa tattacttta 120600 atctgacatt tgcaattgtt gctggctctc taataatcgt cgtagtatca atggagagcc 120660 gagatcatag agtgtttacc agcagctaaa ctatctaacg agctattaaa gatacatgaa 120720 agtaaacata ccgcctgaaa tatttaaatc atagtcagac caaatttgct acaactcgta 120780 ttcacacgaa cttttgacgg tgtatttccc atttaagctt ttgttgctca caaaacactt 120840 ggtatacgta ttaatctcat gagacatgcg atcgtactgt gtacggaggt gtttcgataa 120900 aaagttatgt catatttatc taagtaagcg gataaaaaga taaatatacg caatataaat 120960 gcaacagaaa ttaatggacg gtcttaattt ctgtaaggta acacatcctg acccaaaagt 121020 cataaagtaa tatatttgaa cttctggtca aaccgatcag ggctgttcaa ccaacactta 121080 cgggggctta ttaagaagat tttgttacgg acccacaact gtcgttaggt aactttgaag 121140 tccgtctttt cggatcggcc aaattgactc acctttttga atcttcgcaa gacataacta 121200 atagctcctt tataaaagag taaacactgt ctaaaggtgg aggcccaaga atctttccaa 121260 tttccacatt aatgataact gcactcacgt cacctcttct ttagaacaaa agtaaaaaac 121320 tgccattgca ctaggactta ccgcaaatat tacctcatta tataaattgc agccttaaga 121380 acatcaatag atcataaaaa taaaacatat atcctctatt attaaaatgt aagttcttaa 121440 caaagtacga attagtcaga acttacatta aagagattta gtaggtacac ggtttctgtc 121500 gtataaatta gtttatttca aatgttttcc agtattaatc aatgcctcaa tagcatacaa 121560 ttataatgag actagactat aaacgttagc gacgacagag aggttgtaag cggcattata 121620 gttatctctc cgctctagta tgatacaaaa ggttgccgtt cagatagatt ggtcaataat 121680 ctccctgaac tatcgtctgt atggcggact ttataaatgt agaatcactc taatttaaat 121740 gatggtcagc atttgtgtgc taaaaaaccg tcaaataaag cgtatattcg aaaacaacga 121800 atgtcctgtg aaccatatgc attactagag tactccgcag acaagtatga cataaggctc 121860 cgcaaggcta tttatcgata ctatataaat agatataatc agctattttt ctgtttatat 121920 ccgtaatttt tacgttgttt ataattactt gccagagttt atcactttcc atagcgagga 121980 agccggtata gagtttttcg gtatataagc ttaatgacta cttcgggtag tcccgacaag 122040 atagatctga ataccctcga ataaaactcc aaaaacaatg gctggctgtt ggcagcactg 122100 cattgaaact tcaggcacaa aggcctagct gacttaacca agtggaaaaa cttagaagca 122160 tcctaatatg attatcaacg aaaaacattt tcatttgtga gggatatcca ccaccagata 122220 ctcaaaaacg taataggaat aataactatt aagatgagta cagtggagat gatatcatgt 122280 ttaaataata tggtggtaac gtgatctaga atgcccctta tgagagagta atgtatttaa 122340 cctcggagtc cctgtactta tctactattt atatttcaaa tacaggagtt aacattttaa 122400 cattcaagaa ttgtttcatg cataatcagt cttgaatgta attactgtaa tccataaatg 122460 tgtcaaagac actatatcta atcaagtaaa gtttagaaaa acgcataatc aattacggag 122520 ttatctcatc ttaatattat tgtgatataa tatttgcaat cgctgctgtc tctccaactt 122580 tcgccgtaag atcattagac aggcaagctc ttgttatatt ttacaagggc atgtctaact 122640 atccaatcat aagagggatt cgacagcaga ggtactgcct gaaatatcat catgttagcg 122700 aaattaaatt atgtaccagt cgtaaaaaca cggtttttta gcagtttatt ttgcgtatag 122760 gcttacgtta cttacacgat acctggtatt cgtaatgatc ttatcaagcg tctgttgaaa 122820 cagtattccg aggcgctgcg ttaaattgct atgatatagt tatttttatt agtcgataaa 122880 aagacaatta tagcgattaa gaatgcaact aattataacg aacggcctca aatagtagaa 122940 aatatccctc ctccgggcat ctcctaaaat gccatatatt cggattattg aagatggtta 123000 ccacggcagt tttatctagt ctcataggtc tttatttcga ggttattctt accgactgtg 123060 atgcgtcgtg tcccagcttt gaatgcgata tcaccggatt gtctgacttg gttcaccttt 123120 tagattcctc attgggttat actaactgac acttattctt taagtaagga ctccttattg 123180 gcagtggtga atgagatttg gcattatcct tattattgat aattttactc ctgtcgcatg 123240 aattacagta cgagcttatt ttaccaggat accacttgat cttatggagg atactctttc 123300 aattcttgaa ttggacccac agagatgagt gtactagatg aacgtacaca ttatgtgctc 123360 agttgtataa ttgtgtgttt tcaagaaaga aggtatacgt caatacattc agtatcgaca 123420 taaggtattt ggatagtttc agtgatatag accaatagat ttcagatctc tttacgtatc 123480 agtaaatacc tttatagagt tgattcatat taacactatt ttacatacat caccgacgac 123540 aaaagggttg aaagtggcac tctactaatt tgttcatgtg tgaacaataa ataatattcg 123600 agtacacatt gatgggatag tgttctcgct aggctgtcca cttcgtaatt atcctttgag 123660 tagtgcagac gcattaattt aatatgtggt cagcatttta gcgtgaaatg atggtcaaat 123720 tgtacgcata ccacgttggg atgtatgtgc tatggacttt tagcattacc tgaatcattg 123780 cctatcgatt ttgccataag gcttcgccac gtaattaagt gattccatac ctacaagagt 123840 aatgtgccga atctatgtta atatcaccgt tccttgtgtt aatcaataat ggtagccgct 123900 gcttatcatc aaataaagga aggacacccg aggaggactt tacggtgaat atgccgtata 123960 acttcggcta atcatgttat ctaaataagt tggagtttcc agatataagg gccgaataag 124020 aatcgactat acagcgcagt tcagggccct aactcacgta attgtggcct aacagaccga 124080 atcaagttga aaacctatgg agtagcccaa tatgattgac tgtgaataag aaatttattc 124140 caagggaata accctgatca ttgactctaa actttaatag gaataaagat tagtacaatt 124200 gagaaagcgt acttattgtc atgctcgaat gaaatgctct attagagaat aagttcacgt 124260 cctatgagaa ggttaagaac ttttcctgca tctctcaact tacataatct acccgcatgc 124320 gtaatagacg agtgaacata taaatacaca aaagttcctt ccttcatacg tagttaagca 124380 agtcatagct gtattccata aacctttcaa aatcactgta tcaggttatc taagaactag 124440 agaaatggct agtcatttat ggtaatatct cagttaagtc taaatgcgat aaattgtgta 124500 ttgcggctcc tattttcccg actttaactg tcagatgtta taacaagtac acagtagtta 124560 attgttattt gcttatgtgc aactacgcta tcacgagacc gatccggcag gtgaagcata 124620 taaaagaaac tcatcacatc tacataatta tatcgtacat cggtctaaga atcgtacttt 124680 gcttctaatt ttacatgcgt acggtgcaac tgtatataca cgaaatctaa aaatcacaat 124740 ggactttgta tcaggtatct aaaaggaaat tctgatgccg tgctttaatt cactaaagtg 124800 tggatgttct ctttatacgg ctttgatact attattgtgg caagcaactt aattagttat 124860 taccatcgcc aacgtataat agtttacttc ctcggtgtgg gcccctcctc aaatgccagc 124920 tatagagcat gttgaagccg attagtacaa tagatttttt caaccccaaa cgtcaacagt 124980 cccgctttag tctaagctga tatgtcgggt caagtctcgg gattgagtgc attagcactg 125040 gaatctctcg ctcagttcaa ttaatggata actcatcgag ttatactccg tcacacttat 125100 tctttaaata acgttccgtt attaggatta ataactatcg tttgaaacta ttccaatttc 125160 ttatcgtgtt aagtctttcg caagaaagac agtatgagct tactttacga aataatttct 125220 ttttcaactg caggatactc tactaattct tgaagaagac gtagagtgtt ggatgaatta 125280 gaagccccta cccattttct atcgacttgt atatttatct ctattcaaag atggaagttt 125340 gcatcaattt gttcagtatc gatatagggt gtttcgaaag tattagtgac atagccctat 125400 agattcttca ttaccaaacc gaacactagt tagtattaaa cagtcaattc tgttctacgc 125460 catttaacac ataacgctca agataacaga gctgaatttg agagactaca atattgttta 125520 tgtgtcacca attaacagtt aacgaataca tgacgatgcg gaacacctcg ggccaggccg 125580 cgcacttcct atatttactt tgagcagtgt aggtgtaata atatagcatg tagcctcatg 125640 cttaacttga accggagata aaaatctatg cacgcgacct tcacatttac aaggtttaga 125700 tttttaatgc tacccaaaac aaagtccata ggtttttcat taagactacg gcgcaaagtt 125760 aagtgatttc actcctacaa cagaaatatg cccaaactat gaaaacaaca tggttcgtag 125820 aaataatcaa tcgtgataga ggttgcatat tgtcaaatca aagagccaca ctcggggagc 125880 agttgacgga cgtcatcacg tgcaatttcg gctaatctta taatctataa atgttgaggt 125940 ttgcagttgt cagggcgaga tcaaattcaa gtatatagtc ctgtccagag ccttaattca 126000 catattcgcg acgcttgaaa gcgcgtcatg cttatcactg attcactagc tcaatgtgtg 126060 gcaatctaag taggaagtct gttgcaaggc taaaaaacta aatattgata gctaccttag 126120 ttaaggtaga agaattgcac aattcagtag gcgttttttc tgctatactc gaacggaatg 126180 ctttattaaa gtaaagcttg atgtcccacg agatgaacga gaacttctat aacatctcac 126240 aacctactaa attttggggg atgggtaaaa gttagctgaa catacaaata ggaatttgat 126300 tgtaccctta gacgaaacta aacaaatcat agtaaatccg cacaaagttt acatattaat 126360 tcatacggcg tttcaaagag gcaatggctt gacttgtggt gaattacagt ttgtgagtaa 126420 ccacaagatg cggtaaatac tgtaatccga gtttaattga ctcgacttaa agattctgat 126480 attataatga aaacacaatg gtttattgtc aattacttat gcactgttac atcttgtggt 126540 cctcggtcca gcctgcgtag gatataaatg aagcacgtct catccagatt gatctatcgt 126600 acctccaaat acgaattcaa cttggcgtct atatccagaa atgagcccta gaagtgatag 126660 acttccaaat ctttaaacta ccatgggata tgtttcacgt atccaaaaag taattataat 126720 tctgcgtttc cattcactta agtgaggatg tagcctttat attggtttca tagttttgtt 126780 ataccaagca tgtattttac ctagcactat tttcaaccta aaactgtttt gtttctcgat 126840 gtcaaaccct tgtcagctac ctgcggtggt gcacgaaaaa accgattaga atattaaata 126900 tttacaactc caaattttaa gagccctgct ctagtttaaa ttcatatatc agtataggct 126960 tcgcaattta gtgaataagc ggtatgaatt ttcgcggagt atgaaaagtg actaagtaat 127020 cgagatacat accgttagat agatgcttca gactgcattc tgaatttttg atttataacc 127080 atcgaaggaa tcaatttcat gttcctaacg tgttaagtca tcagtaaaaa agaccataag 127140 ggctcccctt ttgaaataat actgtttcaa actataggct gccctacttg gtgttcaaga 127200 tactatagag tcttcgatga tttaaagccc ccaacccatg ttcaatcaac ttctatgttt 127260 aaccttaaac taacacagga accagctaag atttgtttag catcatttgg gcacgttaca 127320 tatataaaat aaagtatgcc gcagagtttc tcccttaccg aactgtagtc gacctaaagt 127380 ctaatactca ttggtgttgt atcacttttg tgacattagg tatgatttaa ctcttgaaaa 127440 tttttacgac tatgataaaa gattagagct accaaataac tgttaataat catgtcacta 127500 tgtagatcgc caagaatcag ctgggatgaa tgcattattt acatcgtgca aactaggtct 127560 gagtaggtac catcgtagtt tatgcctaag ttgaaccgca aatataggtc ttttgccggg 127620 atctccacta tctgtaaatt gtgaaacctg ttggtaacct atacaaaata catagctata 127680 tcattagtaa taaaacgtaa acgaaagatc attcactcct tcttcggaaa gataaccaaa 127740 gaattaggag aatatggtgc gcacatagaa aggatcggga aataactttg accttcacag 127800 aacaataagt tacaatttgg gaacagtcgg ataacgccac tacgtgctta tttggtttac 127860 cttaaatttt ataattgatg aggattaaaa ttcacgggag aagatctaac ataagtatat 127920 agttatatct gaagcgtttg aacacttact ccctttactt aattaggcct tattgttttc 127980 cctgactcat aagttctatg attggcaatc tatctacaag gagacacgta tcatttaaaa 128040 acaaaaaatt ggtaacttcc ttagttaaag tgctgggaat gctcaaatca gtagtcttct 128100 tctctggaaa tcgcgagggg ggaattttat catgtcgaag tttgttattg gactcattca 128160 accataggct ccatgatatt aaaagagcta gtaaactacg ggggtaggat acaaatttct 128220 tgataataga aatcggaatt cgaatgtgtc cttggtcgac tcagatccaa tccaagtgaa 128280 cccaacgaat gtatatattt tattacatat ggcgtcttaa acagggaatc ccttaacatc 128340 aactggtttt cagataataa gtaaccgcaa catagtgaaa acacagttat cgatactagt 128400 tagagaacct ttaataatgc tacaaccatt ttctaatctt gatgatttgt tgacaattat 128460 tagttcagcg agccatctaa cggttcttag tcaactctac ttatgcaata aatcttgcat 128520 gtttgacgct aactcaacca tggtagcatc aaatacggat acaattaggg gttaatatgc 128580 agaaaatggg cttagggatg atagacactt tacaaattgc acaagcaatc gaaatgtttt 128640 atgtatcgat atagtatgca tttttataga attgccttct agcatgacag aaacaagcct 128700 atctattcgt ttctttatcc tcctatacca cgtgtgtacc tttcccggcc attcattaag 128760 accggaagta tcttgttata caatattaaa cctttaacag cctatcgcgc tgatacacga 128820 attaaccaaa tgaagtttaa aaaattaact actgttattt ttaagtggct tcttcaagat 128880 tgcattcata tatcaataat gacttactaa ggttctaaat gagagaattc aattaatctg 128940 gataaacaaa agggtctgaa gattcataat accaaccatt aaatagacgt tcctctgtgt 129000 atagtaaatc tttatttttt aacctttgaa gaaatcgaca acacgaccct tgcgagtata 129060 gttatgaaaa gaagagatct tcatctttgc ccccttataa tagtacagct tcaaacaata 129120 ttctcagtaa gatggtatgt gtggtactaa gactttcatg actatttgat gtccttattc 129180 tatgtataaa gagtaataat cttaagtctg gagtttactc agcaaccagt tgagtctagg 129240 cacagctttc ttgggttgct tatatattga aaataatgta ctcaacagga tcagtctcat 129300 aaggaattgt gattgaccaa aagtccgcta tgcattgacg tcgtctcagt ttagtaccta 129360 taactatcat cattcttttg gtggctatta caatgttggt aaaagaataa aactacaaca 129420 catctgttaa taatcaactc gctcgattga ttgtcaagaa tgtttagtga tgagtatgtt 129480 atttaaggca ctcgagttgt gattgagttg gatccattgt agtttacggc tatataagtc 129540 ctcaataata cgctctatac gcaaattcct attatctgtg aaatgtttaa cgcgtacgct 129600 aactctataa aatacttaac tatattatac gttagaatat cttaacggac aatcgcattg 129660 tatttatttg tatagattaa tgaaaaagta ggtgcgaata ttgtaggtat ggtaaaggcc 129720 ggtaagtaat tctagccttc atggaacgtt atgttttaat ttgaaattta agggatagtg 129780 cgtctaaatc gttaataaaa ttagttgaaa tcacctagtt agaatctgta aaaattcacc 129840 ataaaagttc caccgtaaat taatgattac tactgtataa ttccaaaatt tactctctta 129900 agttaattaa accaatctgt ttccctaggc ttctataaaa tagcgtcggt aattttcccg 129960 catggagaca gatgttattc ggaaataaaa atttacaaac ttctttggca cttctgctga 130020 caacagtttt gtttatacta ttcttgtcat gatgtagata cgacgcgtta ttatcatgtc 130080 gaagttcata ataagtgtca ttttaccata cagaccatga ttctgaaagt actgacaagc 130140 tgcaaatatg aaattcatat ttctcatcac aaggctacag acctctaata agatgtacgt 130200 taactgagat tcgtgtcggt agaaccgaac gatcatgtaa ctttcactat atgagttgac 130260 ctgctttgag ctttccttta tactaactgg tattctggcg aaacaaagct gtagcagaat 130320 catgtcatga gtattaatag tagtaagaaa atcacggata atgttacaac cgttttctta 130380 tttagaggta gtgtgaagaa cttttacatg aaccagcaaa ctaacatctc tttcaaatta 130440 ctactcatac aataagttcc gtgacgacat gactatctca acctgattta caattaatgc 130500 tcttatattg agaagttatt aagcgagata tgcatttaag gataatagac acatcacaaa 130560 ttgcggatac cattgagaca ttttataaat agatgcaata tggaatctta tagaattgcc 130620 tgatagcgtt atattgatga acatgactaa ttaccctttc acccacgcta ttagcttcca 130680 taccatttgc ggccattcat taagatccgt tgttccttac aaaaaaaaaa ttaataagaa 130740 attccgtaac atgcagattt agtagttatt ataaacaact ttactggaac aaactaaaac 130800 atttataaac agaattttga tagtggcaat taatttctaa tgatgtgata ttaaggtcta 130860 aaatgtaaga attcgataaa ttagattaga caaggggatc cgaagatatt ttagcgcagt 130920 tagttatagg gggaacctct gcctacaaaa agcgtttatt gttaattgtt taaagataca 130980 gtgaaaacga cagtcgttaa aacaaaaatg atatgtacaa tactacatct aagcagcgca 131040 ataacgcaac agcttgaagt atttttcaca gaaaaatcgt acgtccagtt ctaacacttt 131100 catatgtgtt cgtcgtttat gcttttagta tgaaaactag tgtgccggtg tctggagatc 131160 atccttcttg catatgactc caaggacagc catcatggtt tgtaagttca ttgagactgt 131220 tacgcttaac tggacgaaac tcgagaagat atatgattga tcgtaagacc gctttgattc 131280 gacaccatca taacacagta ctcatgatta tcatcatttt tttagtccct attacaaagc 131340 tgccaagtga ataaatctct atcacactct atgaaaatgc acttgatcgt tttattgtag 131400 aaaaaattta ataaacagaa tgttaatgaa ggcactactg tactaataga gttgggctat 131460 atgttaacta caagactata acttattaat aactcggtct atacataatt tggtatcatg 131520 tatgtagtgt tcaagggcta tagcaattct gataaaaatt tattaaggta acaccttaga 131580 ataatatact ggtctatcgc aatacaacca cttctaccgt ataatccgag agtggccacg 131640 ataatcgaag gtatggtcaa ccgttgtaag taattctagg cgataaggaa tccttttttt 131700 ataaattttc tttaggacat tgttcacgta aattatcaag attaattgtt gtaatgagct 131760 cgtttgattt tgaaaatttg tgtcttaaat tcgtctccga ttaatacact atactatact 131820 ttaatcccag atatatcaat attaaactat ttaatttaat ctaatcctca aaccttctat 131880 taaatcgtgt caatcaatat ctgcttccta aatggaagtt tttcgccaaa aacaataaac 131940 agaatttaaa gtcgcttttg tcctcagcaa ttttgttttt tttatacagg ttataacgta 132000 gattcattcc gttaaagcgt tgtccaattg gataaaaaac atctttttaa tctccaggtc 132060 aagtttgtga aagtaaacac gtcgagcaaa tacgtatacc gcacatttga ccacgcagcc 132120 acatacctct ggaaggaaaa acacatacta acctacctga cgctagtgca aaacattcaa 132180 gaaactctga caatgcgaat taacgagcct taaactcttt tatttgctga taagcattat 132240 tgcgaaacta gcttgagctt gtattgtctg gtgtgtacta ttagaagtga aaaaatcagg 132300 gttaatgtct cgtcgcctca tttatataca gatagtcagt gatacctaaa cgtatactag 132360 caaaatgaca tcttaattaa attatttatc ttacatattc ttcctagatg acatgaatat 132420 gttggtccga tgtataattg atgttctgat ataggataat aactgaaaca catatgtatt 132480 ataccaaagt atgtacatca caagttccca atatcgttta gactactatt tactaatccc 132540 attgtggaat cttaatatat gatcggatag ccttgtgttc gtgaaaacgg catattaggc 132600 tcacagcggt gctattacct tccataccag ctagcaacaa cgataatcat ccgctattgg 132660 ataggaaaat agtatttaaa agaaatcttg taacaaatgt tttatatagt cctaattaac 132720 tacattactc gagcgaacta aaacttatga tcacaaaaat aaagctgagc ctaattatgt 132780 gatatgattt aaaatttcgg tctatatagt tataatttga tgaaataaat tggattagca 132840 gtttgctaga tgatttggca cagatagtca tagacgaagg acttactatg aaatagcggt 132900 tttagttgtc aatgttggat ttcagcgaaa gcaggaatcg atgtagctaa agaaatatgt 132960 ccatcattgc atttagataa ggcatcttgc caaaaagaga accaatattt tgtaaagaaa 133020 ctagaggtcc tctttatact ctatgatttg tgcagctctt aaatgcaaac gacgagtaaa 133080 caggtgcgtg ggtgtatgga gaccttcatt tttatgtatg attggatgga ctacgatcac 133140 attttatggg ttctctgcga ctgtcacgct aaattactcg gaaattgaca atgtaatcga 133200 ctacttgtaa taacccactg atcgaactgg agcataacgg gtcacacatc ataattttca 133260 ctattttagt ccgaattatt cagcatcgca gtaaatgtat aacaatcggc cactgtccat 133320 ttacatatca tcgtattcca ttagaattaa tttaatatat agaatgtgta agtaggatct 133380 actgtactta tatgaccatg ctacatatta acatgaaaac tatatccaat tcttgacctt 133440 gtgtatatat aatatgaata cgaacatgtt ggattcaaaa gttatagcaa atcccatctt 133500 aaatgtttag ggaaaggctt tagtattata ttctagccta ttggaacaca agcacctttc 133560 ccatgtaaac ccagtgtctt caccataatg aatactttca tcgatcgtta ttgcttttag 133620 tacatgatga ctcgtccttt taacataaat tttctactga atattcatta caaaatgtac 133680 caggattaac caatgtaatg agcccccttg attttgggta ctggtgattt tatttcgact 133740 cagatttaca cactaaagta aattttaaag ctagacatac aaatattaaa gctctctatt 133800 taacctaaac gataaacaat cagctaaacc gtgtcttact gagtctgctt cttgaatgat 133860 actttaccgc caaagtcaac agttactacc taaagtcgct ttgatctttt gctacaccga 133920 attctttatg aagagagtaa cgtaaatcta ttttgtagaa cgattttttt ctaggtcatg 133980 aaacatttcg ttcatctcta agaaaattat gagacattaa acaggtcaag aattttcgtt 134040 tgctcctcat tagtccacgc acccattttc gtttcgatgt aaaaattcat attagtctat 134100 ctgatgctaa tctaatatac cctagacacg ctgacagagc cattatgtga gccttttact 134160 gttaggttag gtgttgttcg atactgggtg actagcatta cctcgtattg cccagagtgt 134220 ggtatttaaa atggtcaaat taggctctaa aagttgtaac gccatctgca cattgttggc 134280 tggtgaccgg tagatgtata ataatataag gtattcaaaa ttgaattaaa tatgctttgc 134340 gctcatccta catgagataa atatagtgga acgatgcata attctacatt cgattcaggt 134400 tatgaacagg aactcgtatt taaagttttt atcgttcaaa aacctaagtc ttcaataacg 134460 ttttgggtgg gatttgcaaa ccccttacca aaatcgtaat ataggattgg ataaacttgt 134520 cttcgtggta agggtacaat tgggacgctg aagtagtgta acttatgaga aaggctaatt 134580 ataacaaaaa ttaaattcta gtgaggtgga agattgtatt ttaaagatga ctaataaata 134640 ttgttataca tgctcctaat cggttctacg gctcgcgggc accaaaaccc atgacctcta 134700 aaataaagct gagtttagac gcgtgatttc atttaaaatt tcaatctgtt tgtctattat 134760 atccagagat atactgcatc tcctattagt ttatcgattt cgcatacaat gactcagaca 134820 aagaactcac tatggaatcc gagaatcagg tgccaatgat cgttccgatc aaaacatgaa 134880 agctatgtgg cctgacgaat acttctctca ttgtttttag ataaaagatc ttgctgaaat 134940 aggatccagt actttgtaaa gcaagttgag aatcttataa tgttttgaag atcatcgggt 135000 tctaaaaacc aaacgtggac taattaggtg cgtgggtaaa aatctagcta catttctaag 135060 tagtttcaga taaattatga taagtttata tgggatgcgt acgccagtct gcataatact 135120 ttgagagaat gacaatccaa tctagatcaa gctatgaatt acaaacggtt atgaagtaaa 135180 acgggtgtta cagcttaaat ttagccagaa taatttagga gtttcaacat tgccgtagag 135240 gtgtaacaac tcaccagtgg ccatccacat attaatatat tccataactt ttaactaatt 135300 ttatttgtaa cgcgggcaaa atatattttg tttaagtctg cttgctacga tcttagacat 135360 aagcttggtt caatactcgc gcttaaagaa aaatttcaaa aacagcaagt ttttggactc 135420 tcaaattgtt acaaaatgca ccctgtacgt taggggaatg gttactactt tatatccatt 135480 cttgtttaga cagaagcacg attcccatgt taatcctcca acttcattac attgaatata 135540 cgttcccttt aattttctat ttgatttaag gtcattccat catctaacac taaatttcta 135600 ctgattattt ataacaatat gtaggaggat taggcaagat gcgaaatgct ggttgttttt 135660 ggttctggac attttatctc gaattaaatc tgcgcattaa tatgaattct atagattgac 135720 aagcagaaaa cataggtctc tttaagacaa tgggaataat taaataccta gagcataaat 135780 tactgagtaa atatcttgac tcatatcata gattcctagc ccccggttgc taggaaggtt 135840 agtcttgtag tttccataga ccagactgct aaagaagaga gttactgata tctttttttt 135900 agggcgacgt aatcctagat gatgattcat atggaccaac acttagaata ttacaaatct 135960 tctagttacc taatattttt gatttgcacc tgggaaatca ttgcattcga ttttagatcg 136020 gtgtaaagat tgatcaaact ccatttaatt ttatccaaat ttaccatatg catgggataa 136080 cacgtattat gaaattcact tttcgttagg ttacaactag ttcgagactt attgtttgct 136140 aatactacat tatcctcaca ttagcgaatg taattggctc tctaaaaatc ctcatagttg 136200 taatgggaag ccgagattat tgagtgatca ccagtagctg tactatttta taagctatta 136260 aaaattgatt aaaataaaca taccgcctgt aatatataaa tcatagtcag acgaacattg 136320 ctacaatctg tattcgtacc aagttatgag cgcgtatttc ttatttaagt ttttgtcgct 136380 cacaaaacac tgggtttacc aatcattcac atgggacatg cgatcgtact gcctatgatg 136440 atatattggt aagaagttat ctgtctttct tctaagagag caattaaaag gttaaagaaa 136500 tgcaatatat atgcaaggga aattaatgga taatcttaat tcctgtaagg tagcacattg 136560 tgacttaaaa atgactaaat aatattgttg tacatctagc taatccgttc tgggctttac 136620 gaccaacagt aacgaagacc tattaaaaag accttgatat ggacgcgcaa ctatagttaa 136680 gttacctaga tgtccgtctt ttctattcag ccaaattctc ttaccttttt caatctttgg 136740 atctcataat taatagctcg tttatagaag agtatatact atctaagggt ggagggccaa 136800 ggatccttcc aatcacaaca tcaaagatat ctggactgac gttttctcct ctcaaggact 136860 atagaaaaaa aatcccattg cattaggatc taccacaaag attacctggt tgtaaatttt 136920 gcaatgttaa gaacatcaat ggatcataaa aataaaacgt gcatccttta gtaacaaaat 136980 ctaagatcta gacaaatttc caactagtct gaacttagat taaagaggtt taataggtac 137040 acgaactctg tcgtatatat tactttaagt gaaatgtttt ccagtgttga tcaatctctg 137100 aataacaaac gattataatg aaattagagt gtaaacgtta acaacgaccg agagattttt 137160 agcagcatca tagttacctc tcggctctag tatctcacaa atggtcatcg atttgataaa 137220 ttgctcgata atttctatgt actttcattt gtatggcgga ctttataaat ttagtatcag 137280 tctggtttaa acgatgttga gcataagtgt gcttgaaaac tgccacataa agggtaaatt 137340 cgaaaacaac gagtgttttg tgaaccatat gcataattag agtaccctgt acgctagcat 137400 gacacatgcc tccacaaagc tattcttcaa tacagtataa atagattcat tcgtctattt 137460 ttctatttat atgcgttata tttacgttgt ctttaattac ctgctagaat taaagacatt 137520 ccattgtgta ggactgagtt ttcagtaatt cattatatta acttgaagac caattaggca 137580 agacccgaca agttggttgt gaatgcctcc gaataattct tctaaaacta tgcctgggtg 137640 ttgacagcaa tccattgaaa cttcaggcag aaaagcttag ccggtttaag tgaatggaaa 137700 aacttagaag cgttctgtat tgattatcga ggaaatattt tctcatttgt gacagatttc 137760 cacctcccgg ttcttaggaa ggttaaaggt gtaattacta ttgacgtgac tgcagaggag 137820 aagagatctt gttttcattt tttaacggta acgtgatcct gaatggcgtt tataatggag 137880 caatatattt aacgtcggaa ttcttgtagt tatctagtat ttttattttg tatataggag 137940 ataataattt tacattcaag atttgtttca tgcttaatca gtcttgaatg taatttctct 138000 aaatcatcca tgtgccagag acagcatatt taatgaaata aactttacaa aaggtcataa 138060 ttagttaggg agttatcgta tgttaatatt actctgatct gatatttgca atcgctgctg 138120 tctctccaat attcgccgta atatcaatag agaggcgaga tcatactgtg ttttccaacg 138180 gcaagtctat ctaaccagtt attagaggaa cttgatagca gacataccgc ctgaaatatt 138240 tacatcttag tcagattaaa tttactacga ctcgtaaaca cacgattttt tggcagtgta 138300 tttcgcatat aagcttttgt tgcttacagg acacttggta tacgtaatga tctcatgaga 138360 catctgttca tactgtgtac cgaggtgttc cgataaatag ctatgttata tttatctata 138420 taagccgata aaaagacaaa tataggcatt aaaaatgcaa caaatattaa tgaacggtct 138480 caattagtga aaggtatcgc tccttggccc atatctcaaa aagcgatata ttcgaactac 138540 tgatgaagcc catcagggct gttctatcta gacttacggg agcttatttt gaggtttttg 138600 ttacggaccg acaaccgtcg tgacgtaact ttgaagtccg tctttccgga tcgaccgaat 138660 tggttcacct ttttgaatct tcgtaggatt atactaatag ttgctttttg taaaagtaaa 138720 cactccctat aggtggtggt ctatgagttt ttccattatc cttattattg ataattctac 138780 tcatgtcacc tctactatag tacaaattta ttatactacc attgcactag atcttacggc 138840 gaatactctc tcattacata aattggagcc tcagggacat caatagatga taaatataaa 138900 gtttatgtcc tcaattgtaa aattgtaagt tcttaacaaa gtacgtatta gtcagaactt 138960 acattaatga cattaggtat ttacacagtt tctgtgatat agattagttc atttcaaatc 139020 tttttccgta ttagttaatg cctcaataga atagaattat aataacacta tattataaac 139080 gttagcgacg acagagaggt tgaaagcggc attctagtaa tctgtccgtt cgagaacgat 139140 ataaaatgtt cccgtacaga ttgataggtt agtattctcc ctgagctgtc gtctccatga 139200 cggactttat agtagtacaa tcgctttaat ttaatacatg gtcagcattt ctgtgccaaa 139260 aaatcgtcaa ataaagcgta tatccgaata caatgaatgt gctatggacc ataagcatta 139320 ctagaatact ccgcagacaa ctatgtcata aggctccgcg acgcaattta acgatactat 139380 atcaataaaa ataatcagct atttttctgt ttatatcgct aattcttacg ttgattaata 139440 ttgcttgccg gagtttatca tcttttatag ggaggaggcc cgtagaggat tttacggtat 139500 ataagcctaa taacttctac caatggtgcc gtcaaaatag atcagagtat ccagaaataa 139560 aactccaata agaatggctg acactaggca gcacaggatc gaaacttacg ctacaaaggc 139620 ctaacagact gaaccaagtg gaaaaactaa gaagtatccc aatatgatta actgtgaata 139680 agatattcat tcgtgaggaa tatccgtcac cacttactca aaaccgtaat aggaataata 139740 actattaaaa tgaggacagc gtacataatg tcatgctcga ataatatggt cgtatggtga 139800 actagaatac ctcctatgag aaagttaaga acttaacctg ggtgtctctg ttcatatctt 139860 ctatttgcat ctgtaataca ggagtcaaca tattaacaca caagagttct ttcttccata 139920 tgcagttatg taagtcattg ctgtattcca taaacctgtc aaagtcacta tatctggtta 139980 tgtaaagtct agagaaatgc atagtcattt atggaaatat ctcaactaag tataattgtg 140040 ataaaatgta tgtagtggct gctgtttttc caactttcac cgtgagatga ttaaacaagt 140100 acactcttgt tatttattat aagctcatgt gtaactaccc tatcacaaga gcgatccgac 140160 aggtgaagca ttaacaggaa actcatcacg tcagcgtaat taaattatac accagtcgta 140220 aaatcgcact ttattagcag tttaacatgc gtatagtcta accctacata cacgatacct 140280 gaaaatcgta atgaacttag taacggatag ttaaaacggt attccgaagc ggtgcattaa 140340 ttcgctaagg tatggttgtt cttattacac ggcttagata caattatagt ggcaaggaat 140400 acaattagtt attaccaacg gcgacgaata gtagtttatt tccttcctgc gggctcctcc 140460 tgaaatgcca cttatacggc ttattgaagc cgattagtac aatagattta ttcaacctca 140520 aaggtctata ttcccggctt attcttagct gatatgtcgc gtcgagtccc gggtttgagt 140580 gcgttaacac cggattgtct ggcttagttc aacttttgga tacctcatcg ggttatacta 140640 actgacactt attctttaaa taaggttccc ttattgggac tagtaactga gatttgaaat 140700 tatccttatt tctaatgatc ttaactcttt cgcatgaata acagtacgag cttactttac 140760 gaggttatca cttattcaag tgcaggatac tcttccaatt cttgaaaagg acgtagagag 140820 ttgaatgtat tagatgggcg tacgcattat gtgctcactt gtataattgt gtgttttcaa 140880 ggaaggaagt atgcatcaat tcgttcagta tcgacataag gtatttggaa agttttagtg 140940 acatagtcca atagattttt gatctcttta ccgatcagta aataccatta tagagtcaat 141000 tcagatttac gctatttaac acacaacgcc gaggataaaa gggctgaaat tgacagtcta 141060 caatattgtt catgtgtcaa caattaacaa taaacgaata cacgttgatg cgatagtgct 141120 ctggctaggc cgtccacttc gtatattttc tttgagtagt gtagacgtat taatatagca 141180 tgtagccaga ttcttagcat gaaacgatga ttaaaatgta cgcataccac gttgagatgt 141240 atgtgctata gacttttagt gttacctgaa acatagtcca tcgattttgc cataagacta 141300 cggcacgaaa ttaagtgatt tcacacctac aagagaaatg tgccgaatct atgataataa 141360 caccgttcgt tgaattaatc aataatggta gcggttgcat attatcaaat gaaggagcca 141420 cacccgggga ggagtttacg gtcgatatcc cgtacaactt cggctaatca tgttatctaa 141480 ataagttggg gtttgcagtt gtcagggcga aatcagattc gactatacag cccagttcag 141540 agccctaact cacgtaatcg tgaccttaca gagcgagtca agttaattac ctatcgagta 141600 gctcaatatg agagagtgtg aataagaaat ttattgcaag gcaataatcc taattattga 141660 tagcaaactt tgataagaat aaagaatagc acaattcaga aagcgtactt actgtcatac 141720 tcgaatgaaa tgctttatta aagaaaaagt tgacgtccta tgagaagatt aagaacttct 141780 cctgcatctc acaacctact taatcttcgg ggatgggtaa tagataggtg aacatataaa 141840 tagagataag tttctacctt caaacgtagt taaacaagtc atagctatat cccacaaacc 141900 tttcataatc actgtatcgg gatatctaag aagtaatggt atggctagtg atcaatcata 141960 atttgtcagt taagacaaga tgcggtaaat tgtgtattgc gagtcctatt gtctcgactt 142020 aaactgtctg atgttataac aaatacacag tagttaattg tcaattgctt atgtactgct 142080 acgccttgtg gagaccggtc cggcgcgtga aggatataaa tgaaactcgt cacatccaca 142140 ttattatatc gtacatcgga gtacgaatcg aacttggcct ctatttttag atgcgtgcgc 142200 tgcaagtgta aatgtaccaa atctaaaaat tacgatgggt tttgtttcag gtatccaaaa 142260 agtaattctg atgccgcgct tcaattcact aaagtgagga tgttgtcttt atacgggttt 142320 gatactttta ttgtaccaag catctttatt agttagcact atccccaacg tataacagtt 142380 tactttctcg gtgtgagccc ctcgtcaact gcctgcagta gtgcacgttg aagccgatta 142440 gaatattaga ttttttcaac tccaaacgtc aacagtcccg ctctagttta agttcatata 142500 tcaggacagg tctcggaatt aagtgtataa gcgctgggaa ctttcgcgca gttcgaatag 142560 tgagtaagtg atcgagttac acaccgttag attcatcctt cagataacgt tccgtttttt 142620 tgatttataa ctatcgtttg aatcaattcc atcttcttaa cgtgttaagt catccgcaaa 142680 aaagacgata tgagcttacc ttacgaaata atttcatttc gaactacagg gtgctctact 142740 tgctcttgaa gatatcgtag agtgttggat gaattaaaac cccctaccca ttttcaatcg 142800 acttgtatgt ttatccttaa actaacatgg aaatttgctt tgatttgttt agtatcattt 142860 aggcgtgttt caaatgtata attaagtatg ccgcatagtt tctccattac cgaactgaac 142920 accacttagt gtcaaacact cattgctgtt ctacgccatt tatgacatta cgctcaaatt 142980 aactgagctg aatttcaaag actataatat tatttttgtg ttaccaatta acagttaacg 143040 aatacgtgac gatgtagaac accaggagcc aggtcggacg catcctatat ttacttcgtg 143100 cagagtaggt ctaacaagat agcatggagg tttatgctta agttgaaccg cagataaagg 143160 tctatactcg ggatcttcac tatctgaagg tttagaattt taatggtacc ctatacaaag 143220 tgcataggtt tttcattaat attaagacgc aaaggtaagt gaattcactc ctacaacgga 143280 aatatgacca aagtatcaaa acaatatggt tcgtagaaaa aatcgatcgt gataaaggtt 143340 ggattttgac aaaacaaaga gctacagtct gggaacagtc gatggacgcc accacgtgct 143400 ttttcggcta atcttataat ttataaatgt tgaggtttag aattctcggg acgagatcaa 143460 atttaagtat atagtcatat ccgaagcgtt aaatcactta ttcgccatgc ttaaaagcgc 143520 ctcatgcttt tcactgattc attagctcta tgtatggcaa tctatgtacg aagtctgtcg 143580 taagacttaa aaactaaata ttggtagctt ccttagttaa agtacaagga ttgcacaatt 143640 cagtagacat tttttctggt attcccgaac ggaaagcttt attaagacaa agtttgatat 143700 ccgacgggat gaaccacaag ttctatgata tctcagaagc tactaaattt cgggggttgg 143760 gtacaagtta gttgaacata caaattggaa tttgattgta tccttggtcg attctaaaca 143820 aatcgtagta aacccgtgca aagtatatat attatttcat acggcgtttc aaagaggcaa 143880 tggcttgaca tgagctggat ttcagattat gagtaaccac aagatagtgt aaacactgta 143940 atccatattt aattgagatc tcttaaaaat gctgatatta ttttctaaac tcgatggttt 144000 attgacaatt atttgtacag tgatacatct agcggttctt ggtcgaccct acttacgtaa 144060 taaatgtagc acgtttgatc cagactcatc catggtagct tcaaatacgg attcaacttg 144120 gcgtttatat ccagaaaacg gccctagagg tgatagacat ttaacacttt ggacaaccat 144180 tggatatgtt tcacgtatcg atatagtaat tattattctg catttgcttt ctagtaagtg 144240 aggaagaagc ctttctattg gtttcttaat cctcttatac cacgcgtgta tctttcctag 144300 ccctttattg aaactggaag tgtcttgtta ttcaatgtta aacccttgtc agcctattgc 144360 ggtgatgcac gaaaaaacca aatggaatat aaaatattaa ctactcctaa ttttaagtgc 144420 cctcttctag attgtattca tatatcaata tagacttcgc aaactagtga atgagggaaa 144480 tgaatttatc cggaataaca aaagggactg agtattcaag atacaaaccg ttagatagat 144540 gttccagtct gcatagtaaa tttttgtttt ttaaccattg aaggaatcaa tttcacgatc 144600 cttacgagtt tagtcatcag aaaaagagac ctttagcgct ccccccttga aatagtacag 144660 cttcaaacaa taacctgagt aagttggtat ccgaggtact ataaattttt cgatcatttg 144720 atgcccccat cctatgttta aagaactatt atctttagcc ttaagcttac acaggaacca 144780 gctgagtcta ggttaggttc acttgggttg cttacatata taaaataatg tataccgcag 144840 aatttgtccc ttagggaatt gtagttgacc aaaagtctat tactcattgg cgttgtatca 144900 cttttgtgtc aataggtatg atcaatctct tggaaattat tacgatgttg gtaaaagatt 144960 agaactacta aacaactgtt aataatcaag tcgctccgta gattgccaag aatcagttga 145020 gatgaatacg ttatttagaa cgtacaaact acgattgagt tggtaccatc gtagtttatg 145080 cctatgttaa tccccaatta tacgtctttt gcccgaatcc ccactatctg tgaaatgttt 145140 aacgtgttcg ttacctttac aaaatacata gctatatcat acgtaaaaat atcttaacgg 145200 aagatcgtac actctttctt cggatagata agcaaagaaa taggaggata tggtgcacac 145260 atggaaaggg ccggtaagta attctggcct tcatagaaca atatgttata atttggaaat 145320 tgtcggatag cgcgactatg tgcttaattg gtttacttta aattttataa ttgatgacaa 145380 taaaaattca ccggagaagt tctaacgtaa gtatatagtt ataactgaac gattccaaga 145440 tttactccct taagttaatt agacctattt gttttccctg actcctaagt tttatggttg 145500 gcaatttatc tgcaaggaga cacatatcat ttagaaataa aaaattggaa acttctttag 145560 ctgttgtgct gggaacgctc atatcaatac ttttcttctc tagaagttga aaggggggaa 145620 tattatcatg tcgaagtttg ttataagagt cattctacca tacacaccat gattctgaaa 145680 gtgctggtaa actacgggaa taagatacat atttctcatt attagaattc agacctcaaa 145740 tgagtcgttg gtcaactcag atccgaatcg aaagaaccca acgaatatat aacttttatt 145800 acatgtgttg tcttagtcag agtattcctt aacattaact ggttttcaga cgatatgtaa 145860 ctgcagcaga gtcaaatcat ggatattgat agtagttaga gaaccattga taatgttaca 145920 accattttct aattttgatg ttgtgtagac aattattagt tgagcgagct aactaacagt 145980 tcttacaaat ctctactcat acaataaatt ccgtgagctc aacactaact caacctaggt 146040 aacatcaaat gccgatatat tcaggggtta ttatgcgaga tatgcgctta gggataatag 146100 acactttaca aattgcgcat gcgatcgaga tattttatga atcgatataa tatgcattct 146160 tatagaattg cctgttagcg taacataaat aaacgtatct aattactttt ttatccacgc 146220 ttattacatc cgtaccattt ccggccattc attaagatcg gaagtacctt gcaatacaaa 146280 attaaacttt aaatacccta tcacgcagat ttagcaatta ttttaatcaa ctttagtgga 146340 tcaatcatag acatttttaa gtggtatttt caaggtggca tttaaatatt aatgatgaca 146400 tattaaggtt ttaaatgaga gaattcaatt aatttggtta gacaaaggga tccgaagata 146460 ttttatcgca accattaaaa gggcgtacct ctgtctacaa taagccttta tttttaaatg 146520 tttgaagaaa tcgtgaagac gactgttgtc aatacaaata tgataagaac agtactacat 146580 ctttgctccg caataatagt acagcttcaa gtaatattca cagtaaaatg gtatgtctgg 146640 tactaagact ttcatgactg ttcgacgttt atactttaag tataaagagt agtgatccga 146700 tgtctggaga ttattctaca tccaattgac tctaagcaca gccttcttgg cttgctagta 146760 cattgaaagt gatatactca actggacgag actcgaaagg aaatatgatt gaccataaga 146820 ccgctttgtt tcgacatcgt cttagtacag tactcataat tatcatcatt cttttagtgc 146880 ctattacaat gttggcaaaa gaataaatct ccatcacact tcttgaaaat gtacttggtc 146940 gtttgattgt tgagaaagtt taatgatgag tatgttattt aaggcactgg tgtactgata 147000 gagttggact aaatgttaat tacgagtata taactcttca ataattcgct ctatacgcaa 147060 attcctatta tctgtgaaat gtttaacgcc tatggtaact ctgtaaaata tttatctacg 147120 ttatacctta gaatatctta acggactatc gcaatatatc tacttgtact gattaatggg 147180 aaagtaggtg cgataatcga aggtatggta aacgccggta agtaattcta ggcaacaagg 147240 aatgttattt ttttaattat tttttaaggc attgtacgtc taaatcatca ataaaatttg 147300 ttgaaatcac cttgtttgat tttgtaaaaa tttgtcttaa aactatcacc gttaattaaa 147360 gattactaca ctataattcc agaatttaca ttcttaagct atttaaacta atctgtttcc 147420 ctaggcttct ataaaatcgc gtcagtgatt atcccccttg gagacggatg tttttcgcaa 147480 ataacaatta acaaatttct atgtcacttc tgctgtcagc aattttgttt ttactatact 147540 tgttatgatg tagattcgtc gcgttattgc gttgtcgaac ttcataaaaa gtgtcttttt 147600 accatgacca cgcgatataa accccctata tacttggtgt cactctacta gttttatact 147660 tacttcgtgc taattcaaga aatcggtttt gtctcaattt atttgcattc agccgtcaat 147720 acagtatact ctaatgaaga tgactaaacc ttcaatgctt aggcgtgctc taatatagtt 147780 aaccgctatg tcagtctcat tttgtatctc taaaattggg cggtaacata catctccgtc 147840 ttcaataacg acttcttata ttgtattaaa gtcttttatc gtgtatatgt aagccagtgc 147900 gatttctcaa gtttcatcgt ctcactgctc ttctcaaatt gttcgctaat ttggcacata 147960 gtagtaagaa aatcagggat aatgttacaa ccgtttactt atttagaggt agtgtgaaga 148020 acttttacat gaaccagcaa actaacatct cttttaaatt attactctta caataagttc 148080 cgtgacgaca tgattatctc aacctgattt acaattaatg ctcttatatt gaaaagttat 148140 taagcgagat atgcatttaa gcataataga cacatcacaa gttgcggata ccattaagac 148200 attttataaa tagttgcatt atggaatctt atagtataac ctgatagcgt tatattggtg 148260 aagatgacta attacccttt cacccgcgct attagcttcc ataccatttg gcgccattca 148320 ttaagatccg ttgttcctta cgaaaaaaaa attaataaga aattccgtaa catgcagatt 148380 tagtagttat tattaacaac tttactggag caaactaaaa catttataga cagaattttg 148440 gtagaggcaa ttaatatcta atgatgtgat attagggtct aaattgtaag aattcgataa 148500 attaaattag ataaggggat tggaagatat tttagcgcag ttagttatag gcggaacttt 148560 tgcctacaaa aagcgtttat tgttatttgt ctaaagttac agcgaaaaca gcagtcgtta 148620 aaacaaaaat gatatgtgca atactacatc taagcagcgc aataacgcaa cagcttaagg 148680 tatttttcat agaaaaatta taggtccagt tcaaacactt tcatttgtgt tcctcgttta 148740 tgcttttagt atgaaaacta gtgcgccggt gtctggagat catccttctt gcgtatgatt 148800 gcatggactg ccatcatgtt ttgtaagttc attgagactg ttacgcttaa ctgcacgaaa 148860 ttcgagaaaa tatacgactg ttcgtaagag cgctttgatt gaacacgatc ataacagagc 148920 actcatgata atcatcattt ttttagtccc tattaca 148957

Claims (19)

  1. 하나 이상의 샘플 중 전사물 변이체의 조절된 식별 및/또는 정량분석을 위한 방법으로서,
    a) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 상이한 핵산 (NA) 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지는, 전사물 변이체를 모방하는(simulating) 인공 NA 분자의 참조 세트를 제공하고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자가 동일한 인공 유전자의 참조 전사물 변이체이고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자가 적어도 80 뉴클레오티드(nt) 길이의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자가 적어도 80 nt 길이의 적어도 또 다른 서열만큼 상이하며,
    여기에서 상기 NA 분자의 적어도 2개, 바람직하게는 각각이 소정의 몰량으로 존재하는 단계; 및
    b) 상기 참조 세트를 외부 대조용으로서 상기 전사물 변이체를 포함하는 하나 이상의 샘플에 가하는 단계; 및
    c1) NA 서열분석을 리드 생성(read generation) 및 할당(assignment)을 근거 로 수행하고, 여기에서 참조 리드 할당이 상기 참조 세트의 리드에 의해 생성되고 상기 참조 리드 할당을 사용하여 상기 하나 이상의 샘플의 전사물 변이체의 리드 할당을 조절, 확인 또는 변형시키는 단계; 또는
    c2) NA 검출 또는 정량분석 방법, 바람직하게는 마이크로-어레이 분석 또는 qPCR을 상기 하나 이상의 샘플상에서 수행하고, 여기에서 적어도 하나의 탐침이 상기 참조 세트의 적어도 하나의 NA 분자에 결합하며 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 적어도 하나의 탐침으로부터 생성되는 신호에 기반한 측정 결과를 사용하여 상기 NA 검출 또는 정량분석 방법에서 탐침에 결합하는 상기 하나 이상의 샘플의 전사물 변이체로부터 생성되는 신호에 기반한 측정 결과를 조절, 확인 또는 변형시키는 단계
    를 포함하는, 방법.
  2. NA 서열분석 방법을 평가하거나 또는 NA 검출 또는 정량분석 방법을 평가하기 위한 방법으로서,
    a) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 상이한 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지는, 전사물 변이체를 모방하는 인공 NA 분자의 참조 세트를 제공하고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자가 동일한 인공 유전자의 참조 전사물 변이체이고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자가 적어도 80 nt 길이의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자가 적어도 80 nt 길이의 적어도 또 다른 서열만큼 상이하며,
    여기에서 상기 NA 분자의 적어도 2개, 바람직하게는 각각이 소정의 몰량으로 존재하는 단계; 및
    b1) 상기 NA 서열분석 방법을 평가하기 위해서, 리드 생성 및 할당에 기반한 NA 서열분석을 수행하고, 여기에서 참조 리드 할당이 상기 참조 세트의 리드에 의해 생성되는 단계; 또는
    b2) 상기 NA 검출 또는 정량분석 방법을 평가하기 위해서, 상기 참조 세트상에서 상기 NA 검출 또는 정량분석 방법을 수행하고,
    여기에서 적어도 하나의 탐침이 상기 참조 세트의 적어도 하나의 NA 분자에 결합하는 단계; 및
    c) 임의의 단계 b)의 산출 결과, 특히 산출 몰량, 산출 농도, 및/또는 상기 NA 서열분석 방법 평가의 경우에, 상기 참조 세트의 NA 분자 중 적어도 하나의 할당된 리드의 수, 및/또는 상기 참조 세트의 적어도 2개의 NA 분자의 적어도 하나의 비를 상기 소정의 몰량에 비교하고/하거나, 상기 NA 서열분석 방법 평가의 경우에, 할당된 리드의 수 및/또는 상기로부터 계산되거나 예상되는 비 및/또는 산출결과에 비교하는 단계
    를 포함하는, 방법.
  3. 제1항 또는 제2항에 있어서,
    NA가 RNA 또는 DNA, 특히 RNA인 방법.
  4. 전사물 변이체를 모방하는, 인공 NA 분자, 바람직하게는 RNA 또는 DNA 분자의 참조 세트의 생성 방법으로서,
    A) 천연 진핵생물 유전자, 바람직하게는 동물 또는 식물 유전자, 보다 바람직하게는 척추동물 유전자, 훨씬 더 바람직하게는 포유동물 유전자, 특히 인간 유전자의 그룹으로부터 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 유전자를 선택하는 단계; 및
    B) 각각의 선택된 유전자에 대해 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 천연 mRNA 전사물 변이체를 선택하고, 여기에서 각각의 전사물 변이체가 적어도 100 nt의 길이를 가지며 적어도 하나의 엑손을 포함하는 단계; 및
    C) 적어도 하나의 엑손을 포함하는 상기 선택된 천연 mRNA 전사물 변이체 각각의 서열을 제공하고, 임의로 여기에서 상기 서열이 또 다른 NA 유형, 예를 들어 DNA 서열로 전환되는 단계; 및
    D) 각 서열의 각 엑손의 서열을 대략 동일한 길이의 서열로 치환시켜 단계 C)의 각 서열을 하기에 의해 변형시키고,
    여기에서 대략 동일한 길이의 서열을 바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 임의의 다른 역전된 천연 서열, 비-천연 서열, 및 이들의 조합의 그룹 중에서 선택하고, 바람직하게는 상기 대략 동일한 길이의 서열을 바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 비-천연 서열, 및 이들의 조합 중에서 선택하고, 보다 바람직하게는 상기 대략 동일한 길이의 서열을 바이러스 서열, 박테리오파지 서열, 그의 역전된 서열, 및 이들의 조합 중에서 선택하고, 바람직하게는 여기에서 상기 대략 동일한 길이의 서열을, 10개 이하, 바람직하게는 5개 이하, 특히 2개 또는 1개 이하의 디뉴클레오티드를 서로 독립적으로 임의의 다른 디뉴클레오티드에 의해, 바람직하게는 GT, GC 또는 AT에 의해 치환시키고/시키거나 10개 이하, 바람직하게는 5개 이하, 특히 2개 또는 1개 이하의 디뉴클레오티드를 서로 독립적으로 임의의 다른 디뉴클레오티드에 의해, 바람직하게는 AG, AC 또는 AT에 의해 치환시킴으로써 변형시키고,
    상기에 의해 인공 전사물 서열의 세트를 획득하나, 단
    상기 동일한 선택된 유전자의 상기 선택된 천연 mRNA 전사물 변이체의 서열로부터 획득된 인공 전사물 서열은, 바람직하게는 단일 엑손 서열 중에 포함되는, 적어도 80 nt 길이의 서열을 공유해야 하며, 바람직하게는 단,
    단계 C)의 서열의 엑손 서열이 단계 C)의 서열의 또 다른 엑손 서열에 일치하는 경우, 상기 엑손 서열 및 또 다른 엑손 서열은 대략 동일한 길이의 동일한 상기 서열에 의해 치환되어야 하는 단계; 및
    E) 단계 D)의 세트의 인공 전사물 서열 중 적어도 하나를 임의로 중복시키고 상기 중복된 서열을 상기 세트에 가하여, 단계 F) 내지 J) 중 하나 이상에서 선택적 변형을 위한 사본을 포함하는 세트를 획득하는 단계;
    F) 적어도 하나의 서열을 상기 세트의 인공 전사물 서열의 적어도 하나에 임의로 삽입하고,
    여기에서 상기 적어도 하나의 삽입된 서열이 단계 D)의 인공 전사물 서열 중 어느 하나의 동일한 길이의 센스 또는 안티-센스 서열과 일치하는 단계;
    G) 상기 세트의 인공 전사물 서열 중 적어도 하나로부터 1 nt 내지 10000 nt 범위의 길이를 갖는 적어도 하나의 서열을 임의로 제거하고,
    여기에서 상기 하나 이상의 인공 전사물 서열이 각각 적어도 100 nt의 크기로 남아있으며 적어도 하나의 엑손 서열을 포함하는 상태로 남아있는 단계;
    H) 상기 인공 전사물 서열 각각의 첫 번째 뉴클레오티드로서 구아노신을, 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써, 첫 번째 염기를 구아노신으로 교환함으로써 또는 구아노신을 5' 단부에 가함으로써, 바람직하게는 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써, 또는 첫 번째 염기를 구아노신으로 교환함으로써, 특히 5' 단부가 구아노신일 때까지 상기 서열을 5' 절두시킴으로써 임의로 확립시키는 단계;
    I) 상기 인공 전사물 서열의 세트가 GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖도록 상기 세트의 인공 전사물 서열 중 적어도 하나를 임의로 변형시키는 단계;
    J) 바람직하게는 폴리-A 꼬리 서열을, 바람직하게는 적어도 10개, 특히 적어도 20개 아데노신으로 이루어지는 상기 세트의 인공 전사물 서열 중 하나 이상, 바람직하게는 전부에 가하는 단계;
    K) 또는 바람직하게는 단계 E 내지 J 중 적어도 2개의 임의의 조합으로 수행하며, 바람직하게는 여기에서 각각의 방법 단계를 단지 1회만 수행하는 단계; 및
    L) 상기 세트의 각각의 인공 전사물 서열의 경우:
    상기 전체 인공 전사물 서열을 포함하는 NA 분자를 물리적으로 합성하는 단계; 및
    M) 바람직하게는, 단계 L)의 NA 분자가 RNA 분자인 경우, 5' 캡-구조를 상기 RNA 분자에 물리적으로 가하고;
    상기에 의해 전사물 변이체를 모방하는 인공 NA 분자, 바람직하게는 RNA 또는 DNA 분자의 참조 세트를 물리적으로 획득하는 단계
    를 포함하는, 방법.
  5. 제4항에 있어서,
    단계 D) 내지 G), 바람직하게는 모든 단계를, 인공 NA 분자의 참조 세트가 진핵생물 유전자, 바람직하게는 동물 또는 식물 유전자, 보다 바람직하게는 척추동물 유전자, 훨씬 더 바람직하게는 포유동물 유전자, 특히 인간 유전자에 대해 자연에서 발생하는 선택적인 전사 사건을 모방하는 경우에만 수행하고, 상기 사건이 바람직하게는 하기의 그룹 중에서 선택되고/되거나:
    선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된(skipped) 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들;
    상기 인공 NA 분자의 참조 세트가 하기의 그룹 중에서 선택된 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개, 특히 모든 선택적인 전사 사건들을 모방하고/하거나:
    선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들;
    상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 개시 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 GT이며, 여기에서 상기 인트론 개시 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나; 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 종료 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 AT이며, 여기에서 상기 인트론 종료 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나;
    상기 인공 NA 분자의 참조 세트가 500 nt 내지 2000 nt, 바람직하게는 750 nt 내지 1500 nt, 특히 1000 nt 내지 1400 nt의 평균 서열 길이를 가지며; 바람직하게는 300 nt 내지 1200 nt, 바람직하게는 600 nt 내지 900 nt, 특히 700 nt 내지 800 nt의 표준 편차; 적어도 100 nt의 최소 크기; 및 바람직하게는 10000 nt의 최대 크기를 갖고/갖거나;
    상기 인공 NA 분자의 참조 세트가 25% 내지 55%의 평균 GC 함량을 갖고/갖거나;
    상기 인공 NA 분자의 참조 세트가 GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖고/갖거나;
    상기 참조 세트의 각각의 인공 NA 분자가 5' 개시 뉴클레오티드로서 구아노신을 갖고/갖거나;
    상기 참조 세트의 인공 NA 분자 중 적어도 하나, 바람직하게는 각각이 상기가 RNA 분자인 경우 5'-캡 구조를 갖고/갖거나 적어도 10개, 바람직하게는 적어도 20개 아데노신의 폴리-A 꼬리를 갖는,
    방법.
  6. 제4항 또는 제5항에 있어서,
    인공 NA 분자의 참조 세트를 제공함을 또한 포함하며, 여기에서 상기 참조 세트의 NA 분자 중 적어도 2개, 바람직하게는 각각이, 바람직하게는 동일한 용기 중에 소정의 몰량으로 존재하고;
    바람직하게는 상기 NA 분자의 적어도 2개의 각각의 몰량이 적어도 102, 바람직하게는 적어도 103, 보다 바람직하게는 적어도 105, 특히 적어도 106배만큼 상이하며, 특히 상기 NA 분자 중 적어도 2개가 액체 중에 용해된 상태로 제공되거나 또는 액체 중에 용해되거나 희석될 준비가 되어 있으며, 여기에서 그들 각각의 농도 또는 최종 농도 범위가 0.01 아토몰/㎕ 내지 100 펨토몰/㎕, 또는 100 젭토몰/㎕ 내지 1 펨토몰/㎕인,
    방법.
  7. 제4항 내지 제6항 중 어느 한 항에 있어서,
    인공 NA 분자의 참조 세트의 서열이, NCBI GenBank 데이터베이스 수납 번호가 표 3에 나열된, 바람직하게는 표 3 및 표 4 중 어느 하나에 나열된 서열, 특히 바람직하게는 2014년 6월 15일자 발매 NCBI GenBank 데이터베이스 202의 모든 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)가 10-1 미만, 바람직하게는 1 미만, 특히 10 미만이고,
    상기 유사성을 낮은 복잡성 영역의 필터링, 1차 갭 비용 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정하는,
    방법.
  8. 제4항 내지 제7항 중 어느 한 항의 방법에 의해 획득될 수 있는, 전사물 변이체를 모방하는 인공 NA 분자의 참조 세트.
  9. 전사물 변이체, 바람직하게는 RNA 분자 또는 DNA 분자, 특히 RNA 분자를 모방하는 인공 NA 분자의 참조 세트로서,
    A) 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 NA 분자로 이루어지며,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자가 동일한 인공 유전자의 참조 전사물 변이체이고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자가 적어도 80 nt 길이의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자가 적어도 80 nt 길이의 적어도 또 다른 서열만큼 상이하며,
    B) 상기 참조 세트는:
    - 하기의 그룹 중에서 선택된 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개, 특히 모든 선택적인 전사 사건들을 모방하고/하거나:
    선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들;
    - 500 nt 내지 2000 nt, 바람직하게는 750 nt 내지 1500 nt, 특히 1000 nt 내지 1400 nt의 평균 서열 길이를 가지며; 바람직하게는 300 nt 내지 1200 nt, 바람직하게는 600 nt 내지 900 nt, 특히 700 nt 내지 800 nt의 표준 편차; 적어도 100 nt의 최소 크기; 및 바람직하게는 10000 nt의 최대 크기를 갖고/갖거나;
    - 25% 내지 55%의 평균 GC 함량을 갖고/갖거나;
    - GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 랜덤하게 분포된 존재를 갖고/갖거나;
    상기 참조 세트의 각각의 인공 NA 분자가 5' 개시 뉴클레오티드로서 구아노신을 갖고/갖거나;
    상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 개시 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 GT이며, 여기에서 상기 인트론 개시 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나; 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 종료 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 AT이며, 여기에서 상기 인트론 종료 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나;
    상기 참조 세트의 인공 NA 분자의 적어도 하나, 바람직하게는 각각이, RNA 분자인 경우, 5'-캡 구조를 갖고/갖거나, 적어도 10개, 바람직하게는 적어도 20개의 아데노신의 폴리-A 꼬리를 갖고;
    C) 상기 참조 세트의 서열이, NCBI GenBank 데이터베이스 수납 번호가 표 3에 나열된, 바람직하게는 표 3 및 표 4 중 어느 하나에 나열된 서열, 특히 바람직하게는 2014년 6월 15일자 발매 NCBI GenBank 데이터베이스 202의 모든 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)가 10-1 미만, 바람직하게는 1 미만, 특히 10 미만이고,
    상기 유사성을 낮은 복잡성 영역의 필터링, 1차 갭 비용 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정하는,
    참조 세트.
  10. A) 서열번호 1 내지 148의 그룹 중에서 선택된 전체 서열과 적어도 80%, 바람직하게는 적어도 90%, 보다 바람직하게는 적어도 95%, 훨씬 더 바람직하게는 적어도 98%, 특히 100% 일치하는 서열; 또는
    B) 서열번호 156 내지 334의 그룹 중에서 선택된 전체 서열과 적어도 80%, 바람직하게는 적어도 90%, 보다 바람직하게는 적어도 95%, 훨씬 더 바람직하게는 적어도 98%, 특히 100% 일치하는 서열을 갖는 적어도 하나의 엑손을 갖는 서열
    을 포함하는, NA 분자, 바람직하게는 DNA 분자 또는 RNA 분자.
  11. 서열번호 1 내지 148 중에서 선택된 서열의, 80 nt의 최소 크기를 갖는 서열 단편에 적어도 80%, 바람직하게는 적어도 90%, 보다 바람직하게는 적어도 95%, 훨씬 더 바람직하게는 적어도 98%인, 적어도 80개, 바람직하게는 적어도 150개의 연속적인 뉴클레오티드의 서열을 포함하는, NA 분자, 바람직하게는 DNA 분자 또는 RNA 분자.
  12. 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 특히 적어도 5개의 NA 분자 그룹을 포함하고, 이때 각각의 그룹이 적어도 2개, 바람직하게는 적어도 3개, 보다 바람직하게는 적어도 4개, 특히 적어도 5개의 상이한 제10항 또는 제11항의 NA 분자로 이루어지며,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 모든 NA 분자가 동일한 인공 유전자의 참조 전사물 변이체이고,
    여기에서, 각 그룹에 대해 독립적으로, 상기 각 그룹의 NA 분자가 적어도 80 nt 길이의 서열을 공유하고, 상기 각 그룹의 적어도 2개의 NA 분자가 적어도 80 nt 길이의 적어도 또 다른 서열만큼 상이한,
    전사물 변이체를 모방하는 인공 NA 분자의 참조 세트.
  13. 제12항에 있어서,
    상기 참조 세트가:
    - 하기의 그룹 중에서 선택된 적어도 하나, 바람직하게는 적어도 2개, 보다 바람직하게는 적어도 3개, 훨씬 더 바람직하게는 적어도 5개, 특히 모든 선택적인 전사 사건들을 모방하고/하거나:
    선택적인 전사물 개시 부위(TSS), 선택적인 전사물 종료 부위(TES), 안티센스 전사물, 중복 전사물, 및 스키핑된 카세트 엑손(CE), 인트론 유지(IR), 상호 배타적인 엑손(MXE), 선택적인 3' 스플라이싱 부위(A3SS), 선택적인 5' 스플라이싱 부위(A5SS), 선택적인 첫 번째 엑손(AFE), 선택적인 최종 엑손(ALE) 및 트랜스-스플라이싱의 그룹 중에서 선택된 선택적인 스플라이싱 사건들;
    - 500 nt 내지 2000 nt, 바람직하게는 750 nt 내지 1500 nt, 특히 1000 nt 내지 1400 nt의 평균 서열 길이를 가지며; 바람직하게는 300 nt 내지 1200 nt, 바람직하게는 600 nt 내지 900 nt, 특히 700 nt 내지 800 nt의 표준 편차; 적어도 100 nt의 최소 크기; 및 바람직하게는 10000 nt의 최대 크기를 갖고/갖거나;
    - 25% 내지 55%의 평균 GC 함량을 갖고/갖거나;
    - GAA, GAC, GAG, GAT, GCA, GCC, GCG, GCT, GGA, GGC, GGG, GGT, GTA, GTC, GTG, GTT 중에서 선택된 5' 개시 트리뉴클레오티드 또는 AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT 중에서 선택된 5' 개시 디뉴클레오티드 및/또는 AC, AG, AT, CC, CG, CT, GC, GG, GT, TC, TG, TT 중에서 선택된 3' 종료 디뉴클레오티드의 필수적으로 균일하게 분포된 존재를 갖고/갖거나;
    상기 참조 세트의 각각의 인공 NA 분자가 5' 개시 뉴클레오티드로서 구아노신을 갖고/갖거나;
    상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 개시 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 GT이며, 여기에서 상기 인트론 개시 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나; 상기 인공 NA 분자의 참조 세트의 모든 엑손 서열내 모든 인트론 종료 디뉴클레오티드의 적어도 50%, 바람직하게는 적어도 75%, 특히 적어도 95%가 AT이며, 여기에서 상기 인트론 종료 디뉴클레오티드가 각각 상기 참조 세트의 또 다른 인공 NA 분자 중에 존재하지 않는 서열의 5' 말단 디뉴클레오티드이고 이에 의해 상기 또 다른 인공 NA 분자에 대한 인트론을 나타내고/내거나;
    상기 참조 세트의 인공 NA 분자의 적어도 하나, 바람직하게는 각각이, RNA 분자인 경우, 5'-캡 구조를 갖고/갖거나, 적어도 10개, 바람직하게는 적어도 20개의 아데노신의 폴리-A 꼬리를 갖고;
    바람직하게는 상기 참조 세트의 서열이, NCBI GenBank 데이터베이스 수납 번호가 표 3에 나열된, 바람직하게는 표 3 및 표 4 중 어느 하나에 나열된 서열, 특히 바람직하게는 2014년 6월 15일자 발매 NCBI GenBank 데이터베이스 202의 모든 서열과 유사성을 갖지 않으며, 이때 통계학적 유의수준 한계(예상 한계)가 10-1 미만, 바람직하게는 1 미만, 특히 10 미만이고,
    상기 유사성을 낮은 복잡성 영역의 필터링, 1차 갭 비용 및 1, -2의 매치/미스매치 점수와 함께 28의 워드 크기의 매개변수들을 갖는 BLASTn 프로그램에 의해 측정하는,
    참조 세트.
  14. 제9항, 제12항 및 제13항 중 어느 한 항에 있어서,
    NA 분자 중 적어도 2개, 바람직하게는 각각이, 바람직하게는 동일한 용기 중에, 소정의 몰량으로 존재하고;
    바람직하게는 상기 NA 분자의 적어도 2개의 각각의 몰량이 적어도 102, 바람직하게는 적어도 103, 보다 바람직하게는 적어도 105, 특히 적어도 106배만큼 상이하며, 특히 상기 NA 분자 중 적어도 2개가 액체 중에 용해된 상태로 제공되거나 또는 액체 중에 용해되거나 희석될 준비가 되어 있으며, 여기에서 그들 각각의 농도 또는 최종 농도 범위가 0.01 아토몰/㎕ 내지 100 펨토몰/㎕, 또는 100 젭토몰/㎕ 내지 1 펨토몰/㎕인,
    세트.
  15. 제1항 내지 제3항 중 어느 한 항에 있어서,
    참조 세트가 제8항, 제9항, 제12항 및 제13항 중 어느 한 항, 바람직하게는 제12항 또는 제13항, 특히 제13항의 참조 세트이고, 상기 NA 분자 중 적어도 2개, 바람직하게는 각각이 소정의 몰량으로 존재하는, 방법.
  16. 제1항 내지 제3항 중 어느 한 항에 있어서,
    인공 NA 분자의 참조 세트를, 바람직하게는 안정화제와 함께 용기 중에 건조된 상태, 바람직하게는 동결-건조된 상태로 제공하는, 방법.
  17. 제16항에 있어서,
    하나 이상의 샘플 중의 전사물 변이체의 조절된 식별 및/또는 정량분석을 위한 방법을 수행하고, 단계 b)를, 상기 샘플을 용기에 가하고, 이에 의해 상기 샘플 중의 건조된 참조 세트를 용해시킴으로써 수행하는, 방법.
  18. 제4항 내지 제7항 중 어느 한 항에 있어서,
    물리적으로 획득된 참조 세트를 건조, 바람직하게는 동결-건조, 바람직하게는 안정화제와 함께 용기 중에서 건조시키는 단계를 또한 포함하는, 방법.
  19. 제8항, 제9항 및 12항 내지 제14항 중 어느 한 항에 있어서,
    인공 NA 분자의 참조 세트가, 바람직하게는 안정화제와 함께 용기 중에서 건조, 바람직하게는 동결-건조되어 제공되는, 참조 세트.
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