KR20160130380A - 생체분자를 서열화하기 위한 디바이스, 시스템 및 방법 - Google Patents

생체분자를 서열화하기 위한 디바이스, 시스템 및 방법 Download PDF

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KR20160130380A
KR20160130380A KR1020167023002A KR20167023002A KR20160130380A KR 20160130380 A KR20160130380 A KR 20160130380A KR 1020167023002 A KR1020167023002 A KR 1020167023002A KR 20167023002 A KR20167023002 A KR 20167023002A KR 20160130380 A KR20160130380 A KR 20160130380A
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nano
biomolecule
electrodes
channel
gap electrodes
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Korean (ko)
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토모지 카와이
마사테루 타니구치
타카히토 오시로
마크 플로이드 올담
에릭 스코트 노르드만
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퀀텀 바이오시스템즈 가부시키가이샤
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Publication of KR20160130380A publication Critical patent/KR20160130380A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G06F19/22—
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/157—Nanotubes or nanorods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020167023002A 2014-01-24 2015-01-23 생체분자를 서열화하기 위한 디바이스, 시스템 및 방법 Withdrawn KR20160130380A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014011430 2014-01-24
JPJP-P-2014-011430 2014-01-24
PCT/JP2015/052601 WO2015111760A1 (en) 2014-01-24 2015-01-23 Devices, systems and methods for sequencing biomolecules

Publications (1)

Publication Number Publication Date
KR20160130380A true KR20160130380A (ko) 2016-11-11

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KR1020167023002A Withdrawn KR20160130380A (ko) 2014-01-24 2015-01-23 생체분자를 서열화하기 위한 디바이스, 시스템 및 방법

Country Status (6)

Country Link
US (1) US20160377591A1 (enExample)
EP (1) EP3097412A1 (enExample)
JP (1) JP6636455B2 (enExample)
KR (1) KR20160130380A (enExample)
CN (1) CN106414767A (enExample)
WO (1) WO2015111760A1 (enExample)

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US9194838B2 (en) 2010-03-03 2015-11-24 Osaka University Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
EP3578987A1 (en) 2013-09-18 2019-12-11 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
JP2015077652A (ja) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 ナノギャップ電極およびその製造方法
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
KR20180072717A (ko) * 2015-10-08 2018-06-29 퀀텀 바이오시스템즈 가부시키가이샤 핵산 시퀀싱을 위한 장치, 시스템 및 방법
ES2877193T3 (es) 2016-04-27 2021-11-16 Quantum Biosystems Inc Sistemas y métodos para la medición y secuenciación de biomoléculas
WO2018025887A1 (en) * 2016-08-02 2018-02-08 Quantum Biosystems Inc. Devices and methods for creation and calibration of a nanoelectrode pair
US11213825B2 (en) * 2017-05-16 2022-01-04 Wisconsin Alumni Research Foundation System and method for en masse patterning of molecule structures
WO2019000158A1 (zh) * 2017-06-26 2019-01-03 武汉科技大学 一种基于隧道识别技术的纳米检测装置及方法
CN111194408B (zh) 2017-09-29 2022-12-02 帕洛根公司 纳米孔设备及其制造方法
US11740226B2 (en) 2017-10-13 2023-08-29 Analog Devices International Unlimited Company Designs and fabrication of nanogap sensors
JP2021038923A (ja) * 2017-11-02 2021-03-11 株式会社Ｓｃｒｅｅｎホールディングス 基板
US11067561B2 (en) 2017-12-31 2021-07-20 Palogen, Inc. Nanopore device and methods of electrical array addressing and sensing
JP7372683B2 (ja) * 2018-11-06 2023-11-01 国立大学法人大阪大学 電極基板および電極基板の製造方法
US20200326325A1 (en) * 2019-04-12 2020-10-15 Lisa Diamond Nanosensor chip with compound nanopores
US11779918B2 (en) 2019-12-05 2023-10-10 International Business Machines Corporation 3D nanochannel interleaved devices
JP7780783B2 (ja) * 2021-03-04 2025-12-05 国立大学法人大阪大学 デバイス、トンネル電流測定装置、核酸配列読取装置、トンネル電流測定方法、および、核酸配列読取方法
CN113390940B (zh) * 2021-06-03 2022-10-21 浙江大学 一种集成纳米孔的分子隧穿检测装置
US20250321204A1 (en) 2024-04-15 2025-10-16 Robert Bosch Gmbh Sensor unit of a nucleic acid analysis system

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US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
IL148088A0 (en) * 1999-08-13 2002-09-12 Us Genomics Inc Methods and apparatuses for stretching polymers
WO2001063273A2 (en) * 2000-02-22 2001-08-30 California Institute Of Technology Development of a gel-free molecular sieve based on self-assembled nano-arrays
US8232582B2 (en) * 2000-04-24 2012-07-31 Life Technologies Corporation Ultra-fast nucleic acid sequencing device and a method for making and using the same
CN103203256B (zh) * 2006-07-19 2015-09-23 博纳基因技术有限公司 纳米口装置阵列:它们的制备以及在大分子分析中的应用
US8969090B2 (en) * 2010-01-04 2015-03-03 Life Technologies Corporation DNA sequencing methods and detectors and systems for carrying out the same
US8986524B2 (en) * 2011-01-28 2015-03-24 International Business Machines Corporation DNA sequence using multiple metal layer structure with different organic coatings forming different transient bondings to DNA
PT2684027T (pt) * 2011-03-09 2020-01-27 Abionic Sa Rápida quantificação de biomoléculas num biossensor nanofluídico seletivamente funcionalizado e seu método
JP2013090576A (ja) * 2011-10-24 2013-05-16 Hitachi Ltd 核酸分析デバイス及びそれを用いた核酸分析装置

Also Published As

Publication number Publication date
EP3097412A1 (en) 2016-11-30
JP2017509899A (ja) 2017-04-06
WO2015111760A1 (en) 2015-07-30
US20160377591A1 (en) 2016-12-29
JP6636455B2 (ja) 2020-01-29
CN106414767A (zh) 2017-02-15

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